| Evidence type | Key finding | Experimental system/condition | Quantitative details (concentrations, conditions) | Source (first author year) | DOI/URL | Citation ID |
|---|---|---|---|---|---|---|
| domain/topology | **Identity verified for the target protein**: S. pombe Wsc1 is described as a plasma-membrane-associated, serine-rich **cell wall mechanosensor/sensor-like protein**. Wsc-type sensors are characterized by an extracellular WSC/cysteine-rich domain, a long Ser/Thr-rich extracellular region, a single transmembrane segment, and a cytoplasmic tail; this architecture is consistent with the UniProt domain annotation for P87179, although the detailed domain structure is inferred from fungal Wsc-family literature rather than shown directly for S. pombe in the cited primary paper. | Review synthesis for **S. pombe** Wsc1; comparative structural review for fungal Wsc sensors | No direct quantitative measurement given in the cited snippets for S. pombe topology | Cansado 2021; Schöppner 2022 | https://doi.org/10.3390/jof8010032 ; https://doi.org/10.3390/jof8040379 | (pqac-00000006, pqac-00000004) |
| localization | Wsc1 localizes to **active growth sites and the division septum** in S. pombe; primary localization study found **Wsc1p-GFP concentrated in patches at the cell tips**, supporting a polarized cell-surface sensor role. | Wsc1p-GFP localization microscopy in fission yeast | Cell-tip patch localization; review additionally places Wsc1 at growth sites and septum | Cruz 2013; Cansado 2021 | https://doi.org/10.1002/mbo3.113 ; https://doi.org/10.3390/jof8010032 | (pqac-00000000, pqac-00000005) |
| localization | Wsc1 cortical/cell-surface localization is **not altered by microtubule depolymerization**, indicating stable cortical association rather than microtubule-dependent delivery in that assay. | Fluorescence microscopy after microtubule depolymerization | Qualitative result only; no numeric values provided in snippet | Cruz 2013 | https://doi.org/10.1002/mbo3.113 | (pqac-00000001) |
| genetic interaction | **wsc1Δ** and **mtl2Δ** single mutants are viable, but the **double deletion is lethal**; lethality is rescued by overexpression of **Rho1** or its **GEFs**, indicating Wsc1/Mtl2 act upstream of Rho1. | Single and double deletion genetics; suppression by overexpression | Double-mutant lethality; rescue by Rho1 or Rho1-GEF overexpression | Cruz 2013; Cansado 2021 | https://doi.org/10.1002/mbo3.113 ; https://doi.org/10.3390/jof8010032 | (pqac-00000000, pqac-00000005) |
| genetic interaction | Wsc1 **interacts with the Rho-GEF Rgf2p**, linking the sensor to a specific upstream activator of Rho1. | Interaction/functional analysis in S. pombe | Qualitative interaction reported; no affinity/stoichiometry in snippet | Cruz 2013 | https://doi.org/10.1002/mbo3.113 | (pqac-00000000) |
| pathway placement | Wsc1 and Mtl2 **turn on the GTPase Rho1p** and are required to maintain physiological **Rho1-GTP** levels during cell-wall stress. | Stress-response signaling assays in deletion strains | In **wsc1Δ** and **mtl2Δ**, Rho1p-GTP is reduced under cell-wall stress (qualitative in snippet) | Cruz 2013; Cansado 2021 | https://doi.org/10.1002/mbo3.113 ; https://doi.org/10.3390/jof8010032 | (pqac-00000000, pqac-00000005) |
| pathway placement | Wsc1 signaling is consistent with a branch that acts through **Rgf2 → Rho1** to stimulate **glucan synthase / cell wall biosynthesis**, rather than serving as a main upstream activator of the canonical **Pmk1/CIP MAPK** cascade. | Overexpression, genetic interaction, and pathway assays | Overexpression activates cell-wall biosynthesis; no kinetic constants reported | Cruz 2013 | https://doi.org/10.1002/mbo3.113 | (pqac-00000000, pqac-00000002) |
| pathway placement | In S. pombe, **Pmk1/CIP activity remains active** in **wsc1Δ** and **mtl2Δ** under cell-wall stress, indicating Wsc1 is **not an essential authentic upstream component** of the canonical CIP/Pmk1 MAPK module. | Pmk1 phosphorylation/activation assays after multiple stresses in mutant backgrounds | Snippet states Pmk1 phosphorylation was not markedly affected; no fold-change values provided | Cruz 2013; Cansado 2021 | https://doi.org/10.1002/mbo3.113 ; https://doi.org/10.3390/jof8010032 | (pqac-00000002, pqac-00000005) |
| phenotype/assay | **Overproduction of wsc1+** causes **abnormal accumulation of cell-wall material**, **cell growth arrest**, and **morphological abnormalities**, consistent with a positive role in cell-wall biosynthesis signaling. | wsc1+ overexpression in S. pombe | Qualitative phenotype; figure referenced for glucan incorporation/cell-wall accumulation, but no numeric values in snippet | Cruz 2013 | https://doi.org/10.1002/mbo3.113 | (pqac-00000002) |
| phenotype/assay | **wsc1Δ** displays only a **very mild phenotype** under tested conditions, whereas **mtl2Δ** is more stress-sensitive; this supports partial redundancy between Wsc1 and Mtl2. | Deletion mutants under stress assays | Qualitative “very mild vic phenotype” for wsc1Δ in snippet | Cruz 2013 | https://doi.org/10.1002/mbo3.113 | (pqac-00000002) |
| phenotype/assay | Spot assays included cell-wall and stress agents; the study reports testing with **caffeine**, **sodium orthovanadate**, **SDS**, **H2O2**, and **NaCl**, providing an assay framework for Wsc1-related stress phenotyping. | YES plate spot assays with mutant strains | **15 mmol/L caffeine; 1.7 mmol/L sodium orthovanadate; 0.015% SDS; 0.8 mmol/L H2O2; 100 mmol/L NaCl; colonies scored after 3 days at 28°C** | Cruz 2013 | https://doi.org/10.1002/mbo3.113 | (pqac-00000001) |
| applications | Because fungal cell wall integrity signaling controls adaptation to wall damage and the **fungal cell wall is absent in humans**, the Wsc/CWI axis is considered relevant to **antifungal strategy development**; reviews explicitly frame the cell wall/CWI system as an antifungal target area. | Review/translation context across fungi; not a direct Wsc1 intervention study in S. pombe | No S. pombe-specific application metric given in snippet | Cansado 2021; Levin 2005; Yoshimi 2022 | https://doi.org/10.3390/jof8010032 ; https://doi.org/10.1128/mmbr.69.2.262-291.2005 ; https://doi.org/10.3390/jof8050435 | (pqac-00000005, pqac-00000007, pqac-00000008) |
| applications | Wsc-family sensors in fungi are linked to sensitivity/resistance to **cell-wall-targeting agents** (e.g., **caspofungin**, **Congo red**, **Calcofluor white**) in other yeasts/fungi, supporting Wsc-type sensors as informative mechanistic nodes for antifungal screens and cell-wall engineering, though this evidence is not S. pombe-specific. | Comparative fungal structural/industrial reviews | Agents named in snippets: caspofungin, Congo red, Calcofluor white | Schöppner 2022; Yoshimi 2022 | https://doi.org/10.3390/jof8040379 ; https://doi.org/10.3390/jof8050435 | (pqac-00000004, pqac-00000008) |


*Table: This table summarizes experimentally supported and review-supported functional annotation evidence for Schizosaccharomyces pombe Wsc1 (UniProt P87179), covering identity, localization, genetic interactions, pathway placement, phenotypes, and translational relevance. It is useful for distinguishing direct S. pombe evidence from broader fungal Wsc-family inferences.*