Yml6 is the mitochondrial large-subunit ribosomal protein uL4m. It is a conserved structural component of the mitoribosome that supports mitochondrial translation. The uL4 family contributes to the nascent-polypeptide exit-tunnel constriction, a feature retained in the characterized budding-yeast mitoribosome.
Summary: structural constituent of ribosome is supported.
Reason: The supported uL4 family assignment and mitochondrial localization, together with characterized budding-yeast mitoribosome structures (PMID:24675956), justify transfer of the conserved structural/translation role. This is family-based inference, not a target biochemical measurement. The Bot1 paper is contextual support; its cached text does not independently establish every target-specific annotation.
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Summary: structural constituent of ribosome is supported.
Reason: The supported uL4 family assignment and mitochondrial localization, together with characterized budding-yeast mitoribosome structures (PMID:24675956), justify transfer of the conserved structural/translation role. This is family-based inference, not a target biochemical measurement. The Bot1 paper is contextual support; its cached text does not independently establish every target-specific annotation.
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
HDA PMID:16823372 ORFeome cloning and global analysis of protein localization ...
ACCEPT
Summary: mitochondrion is supported.
Reason: Mitochondrial localization is concordant with the conserved mitochondrial ribosomal ortholog assignment. Retain the PomBase genome-scale localization annotation (PMID:16823372); the available abstract describes the screen but does not expose the target image. The source excerpt records the target HDA/IC annotation, not a reinspection of the individual microscopy image.
Reason: Mitochondrial localization is concordant with the conserved mitochondrial ribosomal ortholog assignment. Retain the PomBase genome-scale localization annotation (PMID:16823372); the available abstract describes the screen but does not expose the target image.
Summary: mitochondrial large ribosomal subunit is supported.
Reason: The supported uL4 family assignment and mitochondrial localization, together with characterized budding-yeast mitoribosome structures (PMID:24675956), justify transfer of the conserved structural/translation role. This is family-based inference, not a target biochemical measurement. The Bot1 paper is contextual support; its cached text does not independently establish every target-specific annotation. Target Yml6 was also recovered at low levels with Ppr10 among mitochondrial ribosomal proteins by mass spectrometry (PMID:34119521); this supports translation-machinery association, not an isolated Yml6 activity assay.
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
NAS PMID:18245278 Bot1p is required for mitochondrial translation, respiratory...
ACCEPT
Summary: mitochondrial large ribosomal subunit is supported.
Reason: The supported uL4 family assignment and mitochondrial localization, together with characterized budding-yeast mitoribosome structures (PMID:24675956), justify transfer of the conserved structural/translation role. This is family-based inference, not a target biochemical measurement. The Bot1 paper is contextual support; its cached text does not independently establish every target-specific annotation. Target Yml6 was also recovered at low levels with Ppr10 among mitochondrial ribosomal proteins by mass spectrometry (PMID:34119521); this supports translation-machinery association, not an isolated Yml6 activity assay.
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Summary: ribosome is reviewed in the context of yml6.
Reason: The broad ribosome annotation is compatible with the biology, but mitochondrial large ribosomal subunit captures the supported yml6 function more precisely. Target Yml6 was also recovered at low levels with Ppr10 among mitochondrial ribosomal proteins by mass spectrometry (PMID:34119521); this supports translation-machinery association, not an isolated Yml6 activity assay.
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Summary: ribosome is reviewed in the context of yml6.
Reason: The broad ribosome annotation is compatible with the biology, but mitochondrial large ribosomal subunit captures the supported yml6 function more precisely. Target Yml6 was also recovered at low levels with Ppr10 among mitochondrial ribosomal proteins by mass spectrometry (PMID:34119521); this supports translation-machinery association, not an isolated Yml6 activity assay.
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Summary: translation is reviewed in the context of yml6.
Reason: The broad translation annotation is compatible with the biology, but mitochondrial translation captures the supported yml6 function more precisely. Target Yml6 was also recovered at low levels with Ppr10 among mitochondrial ribosomal proteins by mass spectrometry (PMID:34119521); this supports translation-machinery association, not an isolated Yml6 activity assay.
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Summary: regulation of translation is reviewed in the context of yml6.
Reason: Yml6 is a conserved structural uL4m subunit, but that role does not itself establish regulation of translation. The accessible mitochondrial structural studies do not establish a regulatory mechanism for this target, and the PAINT ancestral regulatory assertion has not been resolved to its experimental grounding. Retain uncertainty rather than equating ordinary ribosome participation with regulatory activity.
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Reason: The supported uL4 family assignment and mitochondrial localization, together with characterized budding-yeast mitoribosome structures (PMID:24675956), justify transfer of the conserved structural/translation role. This is family-based inference, not a target biochemical measurement. The Bot1 paper is contextual support; its cached text does not independently establish every target-specific annotation. Target Yml6 was also recovered at low levels with Ppr10 among mitochondrial ribosomal proteins by mass spectrometry (PMID:34119521); this supports translation-machinery association, not an isolated Yml6 activity assay.
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
NAS PMID:18245278 Bot1p is required for mitochondrial translation, respiratory...
ACCEPT
Summary: mitochondrial translation is supported.
Reason: The supported uL4 family assignment and mitochondrial localization, together with characterized budding-yeast mitoribosome structures (PMID:24675956), justify transfer of the conserved structural/translation role. This is family-based inference, not a target biochemical measurement. The Bot1 paper is contextual support; its cached text does not independently establish every target-specific annotation. Target Yml6 was also recovered at low levels with Ppr10 among mitochondrial ribosomal proteins by mass spectrometry (PMID:34119521); this supports translation-machinery association, not an isolated Yml6 activity assay.
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Core Functions
Provides the conserved uL4m large-subunit structural role in mitochondrial translation.
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)
These computational predictions are reviewed separately from the GOA annotation set used for this review. The assessments below are from this project and do not constitute official GO annotations or endorsement by GO/UniProt. They are not included in the existing annotation review above.
Review rationale: The target has the diagnostic uL4m family domain and mitochondrial localization. Target Yml6 also co-purifies at low levels with Ppr10 among mitoribosomal proteins (PMID:34119521). Characterized S. cerevisiae mitoribosome structures support transfer of the conserved mitochondrial ribosomal role. Translation is therefore supported, and the existing mitochondrial translation annotation (GO:0032543) is more specific within the biological-process aspect. The generic prediction is LSP.
PMID:24675956: "Beyond the entrance, the tunnel is lined by conserved elements (Fig. 6C), including the constriction site formed by uL22 and uL4."
PMID:34119521: "Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)"
PMID:34119521: "Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)"
PMID:34119521: "Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)"
Review rationale: The target has the diagnostic uL4m family domain and mitochondrial localization. Target Yml6 also co-purifies at low levels with Ppr10 among mitoribosomal proteins (PMID:34119521). Characterized S. cerevisiae mitoribosome structures support transfer of the conserved mitochondrial ribosomal role. Membership in the mitochondrial large ribosomal subunit is a supported cellular-component annotation and is more specific than ribonucleoprotein complex. The predicted broad complex membership is LSP.
PMID:24675956: "Beyond the entrance, the tunnel is lined by conserved elements (Fig. 6C), including the constriction site formed by uL22 and uL4."
PMID:34119521: "Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)"
PMID:34119521: "Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)"
PMID:34119521: "Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)"
Review rationale: The target has the diagnostic uL4m family domain and mitochondrial localization. Target Yml6 also co-purifies at low levels with Ppr10 among mitoribosomal proteins (PMID:34119521). Characterized S. cerevisiae mitoribosome structures support transfer of the conserved mitochondrial ribosomal role. Membership in the mitochondrial large ribosomal subunit is a supported cellular-component annotation and is more specific than ribosome. The predicted broad complex membership is LSP.
PMID:24675956: "Beyond the entrance, the tunnel is lined by conserved elements (Fig. 6C), including the constriction site formed by uL22 and uL4."
PMID:34119521: "Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)"
PMID:34119521: "Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)"
PMID:34119521: "Mass spectrometry (MS) analysis of Ppr10-copurified proteins revealed that some proteins were specifically detected in the Ppr10-TAP preparation, albeit at low levels. These proteins included mt-SSU proteins (Nam9, Mrps28, Mrp4, and Mrps9), mt-LSU proteins (Aco2-Mrpl49, Mrpl7, and Yml6), SPAC750.08c (a putative NAD-dependent malic enzyme), SPBP4H10.18c (a Schizosaccharomyces specific protein of unknown function), and Anc1 (a mitochondrial ADP/ATP carrier)"