ant

UniProt ID: P0A0D2
Organism: Staphylococcus aureus
Review Status: DRAFT
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Gene Description

ant encodes an aminoglycoside nucleotidyltransferase in Staphylococcus aureus. UniProt/CARD identify this entry as an aminoglycoside-modifying resistance enzyme that inactivates aminoglycoside antibiotics by adenylylation/nucleotidyl transfer. UniProt names the protein 'Spectinomycin 9-adenylyltransferase' (accession P0A0D2).

Existing Annotations Review

GO Term Evidence Action Reason
GO:0016779 nucleotidyltransferase activity
IEA
GO_REF:0000002 		MODIFY
Summary: The existing MF term is directionally correct but less informative than the family-specific AMR activity aminoglycoside nucleotidyltransferase activity.
Reason: Replace the broad or over-specific electronic term 'nucleotidyltransferase activity' with aminoglycoside nucleotidyltransferase activity based on UniProt/CARD determinant identity and the curated ARO->GO mapping.
Supporting Evidence:
file:genes/STAAU/ant/ant-uniprot.txt
CC -!- FUNCTION: Mediates bacterial resistance to the antibiotic spectinomycin CC but not streptomycin. {ECO:0000269|PubMed:2993813}.
GO:0046677 response to antibiotic
IEA
GO_REF:0000002 		ACCEPT
Summary: The gene product is an antimicrobial-resistance determinant, so the antibiotic-response annotation is retained.
Reason: The CARD/ARO determinant identity supports an antibiotic-resistance biological role.
Supporting Evidence:
file:genes/STAAU/ant/ant-uniprot.txt
CC -!- FUNCTION: Mediates bacterial resistance to the antibiotic spectinomycin CC but not streptomycin. {ECO:0000269|PubMed:2993813}.
GO:0070566 adenylyltransferase activity
IEA
GO_REF:0000002 		MODIFY
Summary: The existing MF term is directionally correct but less informative than the family-specific AMR activity aminoglycoside nucleotidyltransferase activity.
Reason: Replace the broad or over-specific electronic term 'adenylyltransferase activity' with aminoglycoside nucleotidyltransferase activity based on UniProt/CARD determinant identity and the curated ARO->GO mapping.
Supporting Evidence:
file:genes/STAAU/ant/ant-uniprot.txt
CC -!- FUNCTION: Mediates bacterial resistance to the antibiotic spectinomycin CC but not streptomycin. {ECO:0000269|PubMed:2993813}.
GO:0009012 aminoglycoside 3''-adenylyltransferase activity
IDA NOT
PMID:29871922
Structural mechanism of AadA, a dual-specificity aminoglycos... 		ACCEPT
Summary: The negated annotation is retained; this ANT(9) enzyme should not be asserted as aminoglycoside 3''-adenylyltransferase activity.
Reason: UniProt describes this protein as a spectinomycin 9-adenylyltransferase that mediates spectinomycin but not streptomycin resistance, and the PMID:29871922 study distinguishes ANT(9) enzymes from dual ANT(3'')(9) enzymes. Accepting this row preserves the existing NOT qualifier rather than treating the specific 3'' activity as positive evidence.
Supporting Evidence:
file:genes/STAAU/ant/ant-uniprot.txt
CC -!- FUNCTION: Mediates bacterial resistance to the antibiotic spectinomycin CC but not streptomycin. {ECO:0000269|PubMed:2993813}.
PMID:29871922
ANT(9) enzymes, which only mediate resistance to spectinomycin.
GO:0046677 response to antibiotic
IMP
PMID:2993813
Nucleotide sequence of a spectinomycin adenyltransferase AAD... 		ACCEPT
Summary: The gene product is an antimicrobial-resistance determinant, so the antibiotic-response annotation is retained.
Reason: The CARD/ARO determinant identity supports an antibiotic-resistance biological role.
Supporting Evidence:
file:genes/STAAU/ant/ant-uniprot.txt
CC -!- FUNCTION: Mediates bacterial resistance to the antibiotic spectinomycin CC but not streptomycin. {ECO:0000269|PubMed:2993813}.

Core Functions

ant covalently modifies aminoglycoside antibiotics by nucleotidyl transfer, reducing drug activity and contributing to antibiotic resistance.

Molecular Function:
aminoglycoside nucleotidyltransferase activity
Directly Involved In:
response to antibiotic
Supporting Evidence:
  • file:genes/STAAU/ant/ant-uniprot.txt
    CC -!- FUNCTION: Mediates bacterial resistance to the antibiotic spectinomycin CC but not streptomycin. {ECO:0000269|PubMed:2993813}.

References

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Suggested Questions for Experts

Q: Is the ARO-derived aminoglycoside nucleotidyltransferase activity annotation sufficiently specific for ant, or is a narrower substrate/site-specific GO term warranted?

Suggested Experiments

Experiment: Biochemically assay purified ant against representative antibiotic substrates for the inferred AMR family and measure loss of drug activity or target modification.

Type: in vitro enzyme assay

📚 Additional Documentation

Notes

(ant-notes.md)

Notes: ant

Focused AMR batch review. UniProt accession: P0A0D2. Source organism: Staphylococcus aureus.

  • UniProt/CARD provenance: DR CARD; ARO:3002630; ANT(9)-Ia; ARO:0001004; antibiotic inactivation.
  • ARO-to-GO candidate: GO:0034068 (aminoglycoside nucleotidyltransferase activity) from projects/ANTIMICROBIAL_RESISTANCE/data/candidate_new_annotations.tsv and projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml.
  • This is a DRAFT focused review intended to cover the AMR annotation gap; it has not had a gene-specific deep-research pass.

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