apmA

UniProt ID: A0A1D0AST6
Organism: Staphylococcus aureus
Review Status: DRAFT
📝 Provide Detailed Feedback

Gene Description

apmA encodes an aminoglycoside N-acetyltransferase in Staphylococcus aureus. UniProt/CARD identify this entry as an aminoglycoside-modifying resistance enzyme that inactivates aminoglycosides by acetyl transfer. UniProt names the protein 'Aminocyclitol acetyltransferase ApmA' (accession A0A1D0AST6).

Existing Annotations Review

GO Term Evidence Action Reason
GO:0034069 aminoglycoside N-acetyltransferase activity
RCA
file:projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml 		NEW
Summary: NEW candidate annotation from the curated ARO->GO mapping: aminoglycoside N-acetyltransferase activity.
Reason: The UniProt record cross-references a CARD/ARO AMR determinant, and the curated ARO->GO mapping projects the family-specific molecular function aminoglycoside N-acetyltransferase activity. This focused draft accepts the candidate as a curator lead for this AMR determinant.
Supporting Evidence:
file:projects/ANTIMICROBIAL_RESISTANCE/data/candidate_new_annotations.tsv
A0A1D0AST6 ARO:3003918 ARO:3000121 narrower RO:0002327 GO:0034069 aminoglycoside N-acetyltransferase activity
file:genes/STAAU/apmA/apmA-uniprot.txt
DR CARD; ARO:3003918; apmA; ARO:0001004; antibiotic inactivation.
GO:0046677 response to antibiotic
RCA
file:projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml 		NEW
Summary: NEW process annotation: apmA is an AMR determinant involved in antibiotic response/resistance.
Reason: CARD/ARO identity places this gene in an antimicrobial-resistance determinant family; response to antibiotic is the appropriate high-level GO biological process for the resistance role.
Supporting Evidence:
file:genes/STAAU/apmA/apmA-uniprot.txt
DR CARD; ARO:3003918; apmA; ARO:0001004; antibiotic inactivation.

Core Functions

apmA acetylates aminoglycoside antibiotics, reducing drug activity and contributing to antibiotic resistance.

Molecular Function:
aminoglycoside N-acetyltransferase activity
Directly Involved In:
response to antibiotic
Supporting Evidence:
  • file:genes/STAAU/apmA/apmA-uniprot.txt
    DR CARD; ARO:3003918; apmA; ARO:0001004; antibiotic inactivation.

References

file:genes/STAAU/apmA/apmA-uniprot.txt
UniProt flat file for apmA (A0A1D0AST6)
  • UniProt/CARD identity and protein naming for apmA.
    "DR CARD; ARO:3003918; apmA; ARO:0001004; antibiotic inactivation."
file:projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml
Curated ARO to GO mapping set for AMR gene families
  • Manual ARO-to-GO mappings used to identify focused AMR annotation gains.
    "mapping_set_title: ARO to GO mapping (AMR gene families and resistance mechanisms)"
file:projects/ANTIMICROBIAL_RESISTANCE/data/candidate_new_annotations.tsv
Annotation-gain candidates from ARO to GO mappings
  • Candidate GO annotations projected from the ARO-to-GO mapping set.
    "Candidate GO annotations that UniProtKB entries with a CARD cross-reference would gain from the"

Suggested Questions for Experts

Q: Is the ARO-derived aminoglycoside N-acetyltransferase activity annotation sufficiently specific for apmA, or is a narrower substrate/site-specific GO term warranted?

Suggested Experiments

Experiment: Biochemically assay purified apmA against representative antibiotic substrates for the inferred AMR family and measure loss of drug activity or target modification.

Type: in vitro enzyme assay

📚 Additional Documentation

Notes

(apmA-notes.md)

Notes: apmA

Focused AMR batch review. UniProt accession: A0A1D0AST6. Source organism: Staphylococcus aureus.

  • UniProt/CARD provenance: DR CARD; ARO:3003918; apmA; ARO:0001004; antibiotic inactivation.
  • ARO-to-GO candidate: GO:0034069 (aminoglycoside N-acetyltransferase activity) from projects/ANTIMICROBIAL_RESISTANCE/data/candidate_new_annotations.tsv and projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml.
  • This is a DRAFT focused review intended to cover the AMR annotation gap; it has not had a gene-specific deep-research pass.

📄 View Raw YAML

 
id: A0A1D0AST6
gene_symbol: apmA
product_type: PROTEIN
status: DRAFT
taxon:
  id: NCBITaxon:1280
  label: Staphylococcus aureus
description: apmA encodes an aminoglycoside N-acetyltransferase in Staphylococcus aureus. UniProt/CARD identify this entry as an aminoglycoside-modifying resistance enzyme that inactivates aminoglycosides by acetyl transfer. UniProt names the protein 'Aminocyclitol acetyltransferase ApmA' (accession A0A1D0AST6).
existing_annotations:
- term:
    id: GO:0034069
    label: aminoglycoside N-acetyltransferase activity
  evidence_type: RCA
  original_reference_id: file:projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml
  qualifier: enables
  review:
    summary: 'NEW candidate annotation from the curated ARO->GO mapping: aminoglycoside N-acetyltransferase activity.'
    action: NEW
    reason: The UniProt record cross-references a CARD/ARO AMR determinant, and the curated ARO->GO mapping projects the family-specific molecular function aminoglycoside N-acetyltransferase activity. This focused draft accepts the candidate as a curator lead for this AMR determinant.
    additional_reference_ids:
    - file:projects/ANTIMICROBIAL_RESISTANCE/data/candidate_new_annotations.tsv
    - file:genes/STAAU/apmA/apmA-uniprot.txt
    supported_by:
    - reference_id: file:projects/ANTIMICROBIAL_RESISTANCE/data/candidate_new_annotations.tsv
      supporting_text: "A0A1D0AST6\tARO:3003918\tARO:3000121\tnarrower\tRO:0002327\tGO:0034069\taminoglycoside N-acetyltransferase activity"
    - reference_id: file:genes/STAAU/apmA/apmA-uniprot.txt
      supporting_text: DR   CARD; ARO:3003918; apmA; ARO:0001004; antibiotic inactivation.
- term:
    id: GO:0046677
    label: response to antibiotic
  evidence_type: RCA
  original_reference_id: file:projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml
  qualifier: involved_in
  review:
    summary: 'NEW process annotation: apmA is an AMR determinant involved in antibiotic response/resistance.'
    action: NEW
    reason: CARD/ARO identity places this gene in an antimicrobial-resistance determinant family; response to antibiotic is the appropriate high-level GO biological process for the resistance role.
    additional_reference_ids:
    - file:genes/STAAU/apmA/apmA-uniprot.txt
    supported_by:
    - reference_id: file:genes/STAAU/apmA/apmA-uniprot.txt
      supporting_text: DR   CARD; ARO:3003918; apmA; ARO:0001004; antibiotic inactivation.
references:
- id: file:genes/STAAU/apmA/apmA-uniprot.txt
  title: UniProt flat file for apmA (A0A1D0AST6)
  findings:
  - statement: UniProt/CARD identity and protein naming for apmA.
    supporting_text: DR   CARD; ARO:3003918; apmA; ARO:0001004; antibiotic inactivation.
- id: file:projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml
  title: Curated ARO to GO mapping set for AMR gene families
  findings:
  - statement: Manual ARO-to-GO mappings used to identify focused AMR annotation gains.
    supporting_text: 'mapping_set_title: ARO to GO mapping (AMR gene families and resistance mechanisms)'
- id: file:projects/ANTIMICROBIAL_RESISTANCE/data/candidate_new_annotations.tsv
  title: Annotation-gain candidates from ARO to GO mappings
  findings:
  - statement: Candidate GO annotations projected from the ARO-to-GO mapping set.
    supporting_text: Candidate GO annotations that UniProtKB entries with a CARD cross-reference would gain from the
core_functions:
- description: apmA acetylates aminoglycoside antibiotics, reducing drug activity and contributing to antibiotic resistance.
  molecular_function:
    id: GO:0034069
    label: aminoglycoside N-acetyltransferase activity
  directly_involved_in:
  - id: GO:0046677
    label: response to antibiotic
  supported_by:
  - reference_id: file:genes/STAAU/apmA/apmA-uniprot.txt
    supporting_text: DR   CARD; ARO:3003918; apmA; ARO:0001004; antibiotic inactivation.
suggested_questions:
- question: Is the ARO-derived aminoglycoside N-acetyltransferase activity annotation sufficiently specific for apmA, or is a narrower substrate/site-specific GO term warranted?
  experts: []
suggested_experiments:
- description: Biochemically assay purified apmA against representative antibiotic substrates for the inferred AMR family and measure loss of drug activity or target modification.
  experiment_type: in vitro enzyme assay