| Domain name | InterPro ID(s) | Molecular function | Binding partners or substrates | Cellular process / role |
|---|---|---|---|---|
| FCH/F-BAR region (GAS7_F-BAR; includes FCH and F-BAR annotations) | IPR027267; IPR031160; IPR001060; IPR037957 | Membrane-binding and membrane-remodeling module of the BAR superfamily; binds acidic phospholipids through electrostatic interactions, senses/promotes curved membrane geometry, and supports higher-order assembly on membranes | Negatively charged membrane phospholipids; plasma membrane; cytoskeletal systems via associated factors; GAS7 assembly is promoted by Cdc42-, WASP/N-WASP-, WISH-, and Nck-dependent scaffolding | Couples membrane curvature to actin and microtubule organization; supports membrane protrusion formation, neurite outgrowth, growth-cone function, neuronal morphogenesis, and membrane-associated scaffold assembly |
| WW domain | Not listed in the supplied UniProt InterPro set for A0A674PKV4 | Protein-protein interaction domain that recognizes proline-rich ligands; mediates GAS7 association with actin-regulatory machinery rather than direct catalysis | N-WASP proline-rich region (WW-Pro interaction); indirectly linked to Arp2/3 recruitment and actin polymerization machinery | Enables GAS7-dependent actin remodeling during neurite extension and membrane protrusion formation; helps link membrane-bound GAS7 to cytoskeletal signaling |
| Cytoskeleton-associated GAS7 output (domain-integrated function) | Domain-level function inferred from the above annotations | Coordinates membrane remodeling with cytoskeletal assembly; in mammalian GAS7 isoforms, promotes microtubule bundling and cross-linking of microtubules with actin filaments | Microtubules; actin filaments; N-WASP/Arp2/3 pathway components | Neuronal cell morphogenesis, neurite elongation/branching, neuronal migration, and stabilization of protrusive cellular architecture |


*Table: This table summarizes the domain architecture and inferred functions of Takifugu rubripes gas7a based on its UniProt domain annotations and conserved GAS7-family literature. It is useful because direct literature on the pufferfish protein is limited, so functional annotation relies on strong domain-based and ortholog-based evidence.*