| Interacting protein / pathway component | Type of interaction | Functional consequence | Evidence source |
|---|---|---|---|
| N-WASP | Direct binding via WW-Pro interaction | Recruits actin-regulatory machinery to promote membrane protrusions and neurite outgrowth | You et al., 2010. *Gas7 Functions with N-WASP to Regulate the Neurite Outgrowth of Hippocampal Neurons*. J Biol Chem. Published Apr 2010. https://doi.org/10.1074/jbc.M109.051094 |
| Arp2/3 complex | Downstream pathway component recruited through GAS7–N-WASP complex | Drives actin polymerization needed for growth-cone and neurite extension | You et al., 2010. *Gas7 Functions with N-WASP to Regulate the Neurite Outgrowth of Hippocampal Neurons*. J Biol Chem. Published Apr 2010. https://doi.org/10.1074/jbc.M109.051094 |
| Cdc42 | Upstream small GTPase/scaffold-regulated assembly factor | Promotes higher-order assembly of GAS7 on membranes; links GAS7 to curvature-dependent actin-remodeling systems | Noor et al., 2023. *Small GTPase Cdc42, WASP, and scaffold proteins for higher-order assembly of the F-BAR domain protein*. Science Advances. Published Apr 2023. https://doi.org/10.1126/sciadv.adf5143 |
| WISH | Scaffold/adaptor associated with GAS7 assembly pathway | Enhances ordered membrane assembly of GAS7 together with WASP/N-WASP and Cdc42 | Noor et al., 2023. *Small GTPase Cdc42, WASP, and scaffold proteins for higher-order assembly of the F-BAR domain protein*. Science Advances. Published Apr 2023. https://doi.org/10.1126/sciadv.adf5143 |
| Nck | Scaffold/adaptor associated with GAS7 assembly pathway | Supports multivalent assembly of GAS7 at membranes, strengthening membrane-associated signaling complexes | Noor et al., 2023. *Small GTPase Cdc42, WASP, and scaffold proteins for higher-order assembly of the F-BAR domain protein*. Science Advances. Published Apr 2023. https://doi.org/10.1126/sciadv.adf5143 |
| Membrane phospholipids / plasma membrane | Direct binding through F-BAR/FCH region | Membrane association, curvature sensing/generation, and formation of membrane-bound GAS7 assemblies and protrusions | Carman & Dominguez, 2018. *BAR domain proteins—a linkage between cellular membranes, signaling pathways, and the actin cytoskeleton*. Biophysical Reviews. Published Nov 2018. https://doi.org/10.1007/s12551-018-0467-7; Liu et al., 2015. *F-BAR family proteins, emerging regulators for cell membrane dynamic changes—from structure to human diseases*. J Hematol Oncol. Published May 2015. https://doi.org/10.1186/s13045-015-0144-2 |
| Actin filaments | Functional cytoskeletal target; coordinated through N-WASP/Arp2/3 and direct cytoskeletal remodeling outputs | Enables protrusion formation, neurite extension, and neuronal morphogenesis | You et al., 2010. https://doi.org/10.1074/jbc.M109.051094; Gotoh et al., 2013. *Gas7b Regulates Neuronal Cell Morphology by Enhancing Microtubule and Actin Filament Assembly*. J Biol Chem. Published Nov 2013. https://doi.org/10.1074/jbc.M113.513119 |
| Microtubules | Functional cytoskeletal target; co-localization and assembly-promoting relationship | Enhances microtubule bundling and supports dendrite-like process / filopodia formation | Gotoh et al., 2013. *Gas7b Regulates Neuronal Cell Morphology by Enhancing Microtubule and Actin Filament Assembly*. J Biol Chem. Published Nov 2013. https://doi.org/10.1074/jbc.M113.513119 |
| Microtubule–actin interface | Cytoskeletal cross-linking output | Coordinates the two major cytoskeletal systems during neurite morphogenesis | Gotoh et al., 2013. *Gas7b Regulates Neuronal Cell Morphology by Enhancing Microtubule and Actin Filament Assembly*. J Biol Chem. Published Nov 2013. https://doi.org/10.1074/jbc.M113.513119 |
| Neuronal migration / morphogenesis pathway | Cellular process regulated by GAS7 rather than a single direct binder | Alters leading-process branching and neurite outgrowth, influencing cortical neuronal migration and morphogenesis | Zhang et al., 2016. *Growth arrest specific gene 7 is associated with schizophrenia and regulates neuronal migration and morphogenesis*. Molecular Brain. Published May 2016. https://doi.org/10.1186/s13041-016-0238-y |


*Table: This table summarizes the main GAS7 interaction partners and pathway relationships inferred from the retrieved GAS7 and F-BAR literature. It is useful for linking GAS7 domain architecture to its membrane-remodeling and cytoskeletal functions relevant to functional annotation.*