id: F6LAX4 gene_symbol: F6LAX4 taxon: id: NCBITaxon:4565 label: Triticum aestivum status: COMPLETE description: >- The PP2A A-subunit scaffold makes heterodimerization less precise than existing PP2A annotations. Neuronal localizations and protein antigen binding are incompatible with wheat biology; chromosome-related roles remain unresolved. source_documents: - genes/WHEAT/F6LAX4/F6LAX4-uniprot.txt - genes/WHEAT/F6LAX4/F6LAX4-goa.tsv - publications/PMID_17174897.md - publications/PMID_17608567.md - genes/WHEAT/F6LAX4/F6LAX4-hypotheses/prediction-animal-specific-terms/openscientist.md predictions: - source_method: ProtNLM2 source_version: UniProt 2024_06 pilot predicted_term: id: GO:0046982 label: protein heterodimerization activity predicted_term_type: GO_MF review: assessment: LSP confidence_score: 2 summary: >- The target has a PP2A regulatory A-subunit family assignment and HEAT-repeat scaffold architecture, consistent with its curated PP2A-complex IBA. Structural work establishes that the A and C subunits form a heterodimeric core enzyme before association with a regulatory B subunit (PMID:17174897). This conserved interaction supports protein heterodimerization at the AC-core level, but a focused report also confirmed that the more informative description is PP2A A-subunit scaffolding for the ABC heterotrimer. The exact term is absent from the cached annotations and is less informative than the existing protein phosphatase regulator and PP2A-complex calls. supported_by: - reference_id: file:WHEAT/F6LAX4/F6LAX4-uniprot.txt supporting_text: >- ID F6LAX4_WHEAT Unreviewed; 587 AA. ... DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central. ... DR GO; GO:0005829; C:cytosol; IBA:GO_Central. ... DR GO; GO:0005634; C:nucleus; IBA:GO_Central. ... DR GO; GO:0000159; C:protein phosphatase type 2A complex; IBA:GO_Central. ... DR GO; GO:0019888; F:protein phosphatase regulator activity; IBA:GO_Central. ... DR InterPro; IPR051023; PP2A_Regulatory_Subunit_A. ... DR PANTHER; PTHR10648:SF4; PROTEIN PHOSPHATASE 2 (FORMERLY 2A), REGULATORY SUBUNIT A, BETA ISOFORM-RELATED; 1. ... FT DOMAIN 276..351 ... FT /note="Phosphatase PP2A regulatory subunit A/Splicing - reference_id: PMID:17174897 supporting_text: >- The PP2A holoenzyme consists of a heterodimeric core enzyme - reference_id: file:WHEAT/F6LAX4/F6LAX4-hypotheses/prediction-animal-specific-terms/openscientist.md supporting_text: >- the informative MF is protein-phosphatase regulator/PP2A-complex scaffolding, already captured by the existing IBA annotations - source_method: ProtNLM2 source_version: UniProt 2024_06 pilot predicted_term: id: GO:0043025 label: neuronal cell body predicted_term_type: GO_CC review: assessment: NPI confidence_score: 0 summary: >- The target is a Triticum aestivum protein, as established by the sequence record and taxon. Neuronal cell body denotes the soma of a neuron, a cell type absent from wheat. This compartment is therefore incompatible with the target organism and is absent from its cached annotations. The prediction alone does not establish which training example or model mechanism produced the error. error_type: TAXON_CONSTRAINT_VIOLATION supported_by: - reference_id: file:WHEAT/F6LAX4/F6LAX4-uniprot.txt supporting_text: >- ID F6LAX4_WHEAT Unreviewed; 587 AA. ... DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central. ... DR GO; GO:0005829; C:cytosol; IBA:GO_Central. ... DR GO; GO:0005634; C:nucleus; IBA:GO_Central. ... DR GO; GO:0000159; C:protein phosphatase type 2A complex; IBA:GO_Central. ... DR GO; GO:0019888; F:protein phosphatase regulator activity; IBA:GO_Central. ... DR InterPro; IPR051023; PP2A_Regulatory_Subunit_A. ... DR PANTHER; PTHR10648:SF4; PROTEIN PHOSPHATASE 2 (FORMERLY 2A), REGULATORY SUBUNIT A, BETA ISOFORM-RELATED; 1. ... FT DOMAIN 276..351 ... FT /note="Phosphatase PP2A regulatory subunit A/Splicing - reference_id: file:WHEAT/F6LAX4/F6LAX4-hypotheses/prediction-animal-specific-terms/openscientist.md supporting_text: >- plants have **no neurons** - source_method: ProtNLM2 source_version: UniProt 2024_06 pilot predicted_term: id: GO:0007059 label: chromosome segregation predicted_term_type: GO_BP review: assessment: UNC confidence_score: 1 summary: >- The target is a PP2A A-subunit scaffold capable of participating in regulatory holoenzymes. The PP2A structure shows how the A subunit associates with catalytic and regulatory subunits (PMID:17174897); the scaffold need not itself catalyze a reaction to participate in a chromosome-related process. Chromosome segregation occurs in plants, but no specific complex or functional experiment connects this wheat product to it in the inspected sources. The process is absent from the cached annotations and remains uncertain. supported_by: - reference_id: file:WHEAT/F6LAX4/F6LAX4-uniprot.txt supporting_text: >- ID F6LAX4_WHEAT Unreviewed; 587 AA. ... DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central. ... DR GO; GO:0005829; C:cytosol; IBA:GO_Central. ... DR GO; GO:0005634; C:nucleus; IBA:GO_Central. ... DR GO; GO:0000159; C:protein phosphatase type 2A complex; IBA:GO_Central. ... DR GO; GO:0019888; F:protein phosphatase regulator activity; IBA:GO_Central. ... DR InterPro; IPR051023; PP2A_Regulatory_Subunit_A. ... DR PANTHER; PTHR10648:SF4; PROTEIN PHOSPHATASE 2 (FORMERLY 2A), REGULATORY SUBUNIT A, BETA ISOFORM-RELATED; 1. ... FT DOMAIN 276..351 ... FT /note="Phosphatase PP2A regulatory subunit A/Splicing - reference_id: PMID:17174897 supporting_text: >- The PP2A holoenzyme consists of a heterodimeric core enzyme - reference_id: file:WHEAT/F6LAX4/F6LAX4-hypotheses/prediction-animal-specific-terms/openscientist.md supporting_text: >- that role is mediated by the **B56 regulatory subunit** binding Shugoshin - source_method: ProtNLM2 source_version: UniProt 2024_06 pilot predicted_term: id: GO:0043005 label: neuron projection predicted_term_type: GO_CC review: assessment: NPI confidence_score: 0 summary: >- The sequence record identifies the target organism as Triticum aestivum. Neuron projections are neuronal structures, and wheat has no neuronal cells. This localization is therefore biologically incompatible with the target taxon and is absent from the cached annotations. No inference about model frequency bias is needed to establish that incompatibility. error_type: TAXON_CONSTRAINT_VIOLATION supported_by: - reference_id: file:WHEAT/F6LAX4/F6LAX4-uniprot.txt supporting_text: >- ID F6LAX4_WHEAT Unreviewed; 587 AA. ... DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central. ... DR GO; GO:0005829; C:cytosol; IBA:GO_Central. ... DR GO; GO:0005634; C:nucleus; IBA:GO_Central. ... DR GO; GO:0000159; C:protein phosphatase type 2A complex; IBA:GO_Central. ... DR GO; GO:0019888; F:protein phosphatase regulator activity; IBA:GO_Central. ... DR InterPro; IPR051023; PP2A_Regulatory_Subunit_A. ... DR PANTHER; PTHR10648:SF4; PROTEIN PHOSPHATASE 2 (FORMERLY 2A), REGULATORY SUBUNIT A, BETA ISOFORM-RELATED; 1. ... FT DOMAIN 276..351 ... FT /note="Phosphatase PP2A regulatory subunit A/Splicing - reference_id: file:WHEAT/F6LAX4/F6LAX4-hypotheses/prediction-animal-specific-terms/openscientist.md supporting_text: >- plants have **no neurons** - source_method: ProtNLM2 source_version: UniProt 2024_06 pilot predicted_term: id: GO:0000775 label: chromosome, centromeric region predicted_term_type: GO_CC review: assessment: UNC confidence_score: 1 summary: >- The target PP2A scaffold can acquire specific localization through the regulatory complexes it assembles. Structural evidence for the PP2A A-B-C complex (PMID:17174897) does not identify a centromere-associated complex containing this wheat product. Plant chromosomes have centromeres, so the localization is biologically possible rather than taxon-inappropriate. It is absent from the cached annotations, and compartment-specific evidence is needed. supported_by: - reference_id: file:WHEAT/F6LAX4/F6LAX4-uniprot.txt supporting_text: >- ID F6LAX4_WHEAT Unreviewed; 587 AA. ... DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central. ... DR GO; GO:0005829; C:cytosol; IBA:GO_Central. ... DR GO; GO:0005634; C:nucleus; IBA:GO_Central. ... DR GO; GO:0000159; C:protein phosphatase type 2A complex; IBA:GO_Central. ... DR GO; GO:0019888; F:protein phosphatase regulator activity; IBA:GO_Central. ... DR InterPro; IPR051023; PP2A_Regulatory_Subunit_A. ... DR PANTHER; PTHR10648:SF4; PROTEIN PHOSPHATASE 2 (FORMERLY 2A), REGULATORY SUBUNIT A, BETA ISOFORM-RELATED; 1. ... FT DOMAIN 276..351 ... FT /note="Phosphatase PP2A regulatory subunit A/Splicing - reference_id: PMID:17174897 supporting_text: >- The PP2A holoenzyme consists of a heterodimeric core enzyme - reference_id: file:WHEAT/F6LAX4/F6LAX4-hypotheses/prediction-animal-specific-terms/openscientist.md supporting_text: >- that role is mediated by the **B56 regulatory subunit** binding Shugoshin - source_method: ProtNLM2 source_version: UniProt 2024_06 pilot predicted_term: id: GO:1990405 label: protein antigen binding predicted_term_type: GO_MF review: assessment: NPI confidence_score: 0 summary: >- The wheat product is a PP2A A-subunit scaffold. The human PP2A A-small-t structure establishes an inhibitor interaction (PMID:17608567); the viral protein's name small t antigen does not demonstrate immunologic protein-antigen recognition by the scaffold. A focused report likewise found this to be an animal/adaptive-immune text-transfer artifact from mammalian PP2A literature, and the term is absent from the cached wheat annotations. This does not support protein antigen binding for a plant PP2A scaffold. error_type: TAXON_CONSTRAINT_VIOLATION supported_by: - reference_id: file:WHEAT/F6LAX4/F6LAX4-uniprot.txt supporting_text: >- ID F6LAX4_WHEAT Unreviewed; 587 AA. ... DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central. ... DR GO; GO:0005829; C:cytosol; IBA:GO_Central. ... DR GO; GO:0005634; C:nucleus; IBA:GO_Central. ... DR GO; GO:0000159; C:protein phosphatase type 2A complex; IBA:GO_Central. ... DR GO; GO:0019888; F:protein phosphatase regulator activity; IBA:GO_Central. ... DR InterPro; IPR051023; PP2A_Regulatory_Subunit_A. ... DR PANTHER; PTHR10648:SF4; PROTEIN PHOSPHATASE 2 (FORMERLY 2A), REGULATORY SUBUNIT A, BETA ISOFORM-RELATED; 1. ... FT DOMAIN 276..351 ... FT /note="Phosphatase PP2A regulatory subunit A/Splicing - reference_id: PMID:17608567 supporting_text: >- ST directly interacts with the PP2A scaffolding A subunit - reference_id: file:WHEAT/F6LAX4/F6LAX4-hypotheses/prediction-animal-specific-terms/openscientist.md supporting_text: >- plants have **no adaptive immune system / antibodies** references: - id: file:WHEAT/F6LAX4/F6LAX4-uniprot.txt title: F6LAX4-uniprot.txt - id: PMID:17174897 title: Structure of the protein phosphatase 2A holoenzyme. - id: PMID:17608567 title: Structural basis of PP2A inhibition by small t antigen. - id: file:WHEAT/F6LAX4/F6LAX4-hypotheses/prediction-animal-specific-terms/openscientist.md title: 'OpenScientist focused report: F6LAX4 animal-specific ProtNLM terms'