btuE

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0034599 cellular response to oxidative stress	IBA GO_REF:0000033	ACCEPT	Summary: ACCEPT. Phylogenetic inference from orthologs in multiple organisms supports involvement of glutathione peroxidase-family proteins in cellular response to oxidative stress. Reason: This IBA annotation is derived from phylogenetic inference (PANTHER) across a set of experimentally characterized orthologs from diverse model organisms including yeast (SGD), mouse (MGI), worm (WB), and fission yeast (PomBase). The conserved domain architecture of Q8P365 is fully consistent with glutathione peroxidase family membership, and the broader literature supports that bacterial GPx-family proteins participate in oxidative stress defense. While no X. campestris-specific experiment has been reported, the phylogenetic evidence is well-grounded. Supporting Evidence: file:XANCP/Q8P365/Q8P365-uniprot.txt Belongs to the glutathione peroxidase family. file:XANCP/Q8P365/Q8P365-deep-research-falcon.md bacterial glutathione peroxidases generally function in peroxide detoxification, reducing hydrogen peroxide and/or organic hydroperoxides and thereby contributing to oxidative-stress defense.
GO:0004601 peroxidase activity	IEA GO_REF:0000002	MODIFY	Summary: MODIFY. The annotation to the broad peroxidase activity term is correct but imprecise; glutathione peroxidase activity (GO:0004602) is more appropriate given the domain and family assignment. Reason: InterPro maps IPR000889 (Glutathione_peroxidase) to the general peroxidase activity term GO:0004601. However, the protein is specifically classified in the glutathione peroxidase family with the GSHPx Pfam domain (PF00255) and the GPX active site motif (IPR029759). The more specific term GO:0004602 (glutathione peroxidase activity) better reflects the predicted enzymatic function. This is a standard InterPro2GO mapping limitation where the mapping is broader than the family assignment warrants. Proposed replacements: glutathione peroxidase activity Supporting Evidence: file:XANCP/Q8P365/Q8P365-uniprot.txt RecName: Full=Glutathione peroxidase {ECO:0000256\|RuleBase:RU000499}; file:XANCP/Q8P365/Q8P365-uniprot.txt Belongs to the glutathione peroxidase family. file:XANCP/Q8P365/Q8P365-uniprot.txt Pfam; PF00255; GSHPx; 1.
GO:0006979 response to oxidative stress	IEA GO_REF:0000002	KEEP AS NON CORE	Summary: KEEP_AS_NON_CORE. This is a broader parent of GO:0034599 (cellular response to oxidative stress) which is already annotated via IBA. Biologically consistent but redundant with the more specific IBA annotation. Reason: GO:0006979 (response to oxidative stress) is an ancestor of GO:0034599 (cellular response to oxidative stress). The IBA annotation to the more specific child term already captures the biology. Retaining this broader IEA annotation is not incorrect but adds no new information; it is kept as non-core. Supporting Evidence: file:XANCP/Q8P365/Q8P365-uniprot.txt Belongs to the glutathione peroxidase family. file:XANCP/Q8P365/Q8P365-deep-research-falcon.md The domain architecture of Q8P365 is consistent with this interpretation, but this should be treated as an inference rather than a confirmed species-specific function.
GO:0098869 cellular oxidant detoxification	IEA GO_REF:0000108	ACCEPT	Summary: ACCEPT. Logical inference from peroxidase activity to cellular oxidant detoxification is well-founded for a glutathione peroxidase. Reason: This annotation is derived from GO logical inference (GO_REF:0000108): proteins with peroxidase activity (GO:0004601) are inferred to be involved in cellular oxidant detoxification (GO:0098869). For a glutathione peroxidase-family protein, this inference is biologically sound. GPx enzymes reduce reactive oxygen species and organic hydroperoxides, which is precisely what cellular oxidant detoxification describes. This represents the core biological process role of the enzyme. Supporting Evidence: file:XANCP/Q8P365/Q8P365-uniprot.txt RecName: Full=Glutathione peroxidase {ECO:0000256\|RuleBase:RU000499}; file:XANCP/Q8P365/Q8P365-deep-research-falcon.md a predicted glutathione peroxidase-family antioxidant enzyme likely involved in cellular peroxide defense.

GO Term

Evidence

Action

Reason

GO:0034599 cellular response to oxidative stress

IBA
GO_REF:0000033

Summary: ACCEPT. Phylogenetic inference from orthologs in multiple organisms supports involvement of glutathione peroxidase-family proteins in cellular response to oxidative stress.

Reason: This IBA annotation is derived from phylogenetic inference (PANTHER) across a set of experimentally characterized orthologs from diverse model organisms including yeast (SGD), mouse (MGI), worm (WB), and fission yeast (PomBase). The conserved domain architecture of Q8P365 is fully consistent with glutathione peroxidase family membership, and the broader literature supports that bacterial GPx-family proteins participate in oxidative stress defense. While no X. campestris-specific experiment has been reported, the phylogenetic evidence is well-grounded.

Supporting Evidence:

file:XANCP/Q8P365/Q8P365-uniprot.txt

Belongs to the glutathione peroxidase family.

file:XANCP/Q8P365/Q8P365-deep-research-falcon.md

bacterial glutathione peroxidases generally function in peroxide detoxification, reducing hydrogen peroxide and/or organic hydroperoxides and thereby contributing to oxidative-stress defense.

GO:0004601 peroxidase activity

IEA
GO_REF:0000002

MODIFY

Summary: MODIFY. The annotation to the broad peroxidase activity term is correct but imprecise; glutathione peroxidase activity (GO:0004602) is more appropriate given the domain and family assignment.

Reason: InterPro maps IPR000889 (Glutathione_peroxidase) to the general peroxidase activity term GO:0004601. However, the protein is specifically classified in the glutathione peroxidase family with the GSHPx Pfam domain (PF00255) and the GPX active site motif (IPR029759). The more specific term GO:0004602 (glutathione peroxidase activity) better reflects the predicted enzymatic function. This is a standard InterPro2GO mapping limitation where the mapping is broader than the family assignment warrants.

Proposed replacements: glutathione peroxidase activity

Supporting Evidence:

file:XANCP/Q8P365/Q8P365-uniprot.txt

RecName: Full=Glutathione peroxidase {ECO:0000256|RuleBase:RU000499};

file:XANCP/Q8P365/Q8P365-uniprot.txt

Belongs to the glutathione peroxidase family.

file:XANCP/Q8P365/Q8P365-uniprot.txt

Pfam; PF00255; GSHPx; 1.

GO:0006979 response to oxidative stress

IEA
GO_REF:0000002

KEEP AS NON CORE

Summary: KEEP_AS_NON_CORE. This is a broader parent of GO:0034599 (cellular response to oxidative stress) which is already annotated via IBA. Biologically consistent but redundant with the more specific IBA annotation.

Reason: GO:0006979 (response to oxidative stress) is an ancestor of GO:0034599 (cellular response to oxidative stress). The IBA annotation to the more specific child term already captures the biology. Retaining this broader IEA annotation is not incorrect but adds no new information; it is kept as non-core.

Supporting Evidence:

file:XANCP/Q8P365/Q8P365-uniprot.txt

Belongs to the glutathione peroxidase family.

file:XANCP/Q8P365/Q8P365-deep-research-falcon.md

The domain architecture of Q8P365 is consistent with this interpretation, but this should be treated as an inference rather than a confirmed species-specific function.

GO:0098869 cellular oxidant detoxification

IEA
GO_REF:0000108

Summary: ACCEPT. Logical inference from peroxidase activity to cellular oxidant detoxification is well-founded for a glutathione peroxidase.

Reason: This annotation is derived from GO logical inference (GO_REF:0000108): proteins with peroxidase activity (GO:0004601) are inferred to be involved in cellular oxidant detoxification (GO:0098869). For a glutathione peroxidase-family protein, this inference is biologically sound. GPx enzymes reduce reactive oxygen species and organic hydroperoxides, which is precisely what cellular oxidant detoxification describes. This represents the core biological process role of the enzyme.

Supporting Evidence:

file:XANCP/Q8P365/Q8P365-uniprot.txt

RecName: Full=Glutathione peroxidase {ECO:0000256|RuleBase:RU000499};

file:XANCP/Q8P365/Q8P365-deep-research-falcon.md

a predicted glutathione peroxidase-family antioxidant enzyme likely involved in cellular peroxide defense.

Core Functions

Predicted glutathione peroxidase that reduces hydrogen peroxide and organic hydroperoxides using glutathione or a thiol reductant, protecting the cell from oxidative damage.

Molecular Function:

glutathione peroxidase activity

Directly Involved In:

cellular oxidant detoxification

Supporting Evidence:

file:XANCP/Q8P365/Q8P365-uniprot.txt
RecName: Full=Glutathione peroxidase {ECO:0000256|RuleBase:RU000499};
file:XANCP/Q8P365/Q8P365-uniprot.txt
Belongs to the glutathione peroxidase family.
file:XANCP/Q8P365/Q8P365-deep-research-falcon.md
bacterial glutathione peroxidases generally function in peroxide detoxification, reducing hydrogen peroxide and/or organic hydroperoxides and thereby contributing to oxidative-stress defense.

External Prediction Reviews

These computational predictions are reviewed separately from the GOA annotation set used for this review. The assessments below are from this project and do not constitute official GO annotations or endorsement by GO/UniProt. They are not included in the existing annotation review above.

ProtNLM2 External predictions

View prediction review YAML · Q8P365-protnlm-predictions-review.yaml · Review status: COMPLETE

The BtuE glutathione-peroxidase family supports oxidative-stress protection. The predicted response is broader than the existing cellular-response annotation.

Source documents: genes/XANCP/Q8P365/Q8P365-uniprot.txt · genes/XANCP/Q8P365/Q8P365-goa.tsv · publications/PMID_20621065.md

Review score: 2 = concordant with evidence; 1 = uncertain; 0 = discordant with evidence. This is an assessment score, not a model probability.

GO:0006979 response to oxidative stress GO_BP

LSP — Less precise than existing annotation Review score: 2/2

Prediction method: ProtNLM2 · Version: UniProt 2024_06 pilot

Review rationale: The target has the BtuE subfamily assignment, glutathione-peroxidase domain, and peroxidase active-site signature. Purified E. coli BtuE reduces peroxides and its expression responds to oxidative stress (PMID:20621065), supporting transfer of the broad protective role. The target already has a curated IBA for cellular response to oxidative stress, which is more specific than response to oxidative stress. The prediction is supported but less precise; annotation overlap does not establish training-data contamination.

Supporting Evidence:

file:XANCP/Q8P365/Q8P365-uniprot.txt: "ID Q8P365_XANCP Unreviewed; 181 AA. ... DR GO; GO:0034599; P:cellular response to oxidative stress; IBA:GO_Central. ... DR InterPro; IPR000889; Glutathione_peroxidase. ... DR InterPro; IPR029759; GPX_AS. ... DR PANTHER; PTHR11592:SF40; THIOREDOXIN_GLUTATHIONE PEROXIDASE BTUE; 1. ... FT ACT_SITE 37"
PMID:20621065: "Purified BtuE decomposes in vitro hydrogen peroxide in a glutathione-dependent manner"

Gene Description

Existing Annotations Review

Core Functions

Predicted glutathione peroxidase that reduces hydrogen peroxide and organic hydroperoxides using glutathione or a thiol reductant, protecting the cell from oxidative damage.

References

External Prediction Reviews

ProtNLM2 External predictions

Deep Research

Falcon

📄 View Raw YAML