LOC101732730

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0005634 nucleus	IBA GO_REF:0000033	ACCEPT	Summary: Nuclear localization is well-supported for a C2H2 zinc finger transcription factor. The protein contains 8 tandem C2H2 zinc finger domains that mediate sequence-specific DNA binding, which requires nuclear localization. UniProt ARBA annotation also predicts nuclear subcellular location. The IBA annotation is based on PANTHER phylogenetic inference from numerous characterized nuclear C2H2-ZNF orthologs across vertebrates. The qualifier "is_active_in" (meaning it carries out its function in the nucleus) is appropriate for a transcription factor. Reason: Nuclear localization is the expected and strongly supported location for a C2H2 zinc finger transcription factor. The phylogenetic inference from multiple well-characterized nuclear zinc finger protein orthologs provides robust support. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN001225435 · PTHR24381 Eumetazoa-level PAINT node (IBD for nucleus) SUPPORTS TRANSFER Node assessed SOUND in interpro/panther/PTHR24381/PTHR24381-review.yaml (paint snapshot 20260529): the IBD is seeded by 39 experimentally annotated eumetazoan members, nuclear localization follows from the family's conserved DNA-binding architecture, and no diverged non-nuclear branch is documented anywhere in the cached family data. MGI:MGI:1916754 · mouse Zfp932 SUPPORTS TRANSFER Seed inspected: identity verified via Alliance/mygene.info; carries IDA GO:0005634 (PMID:21177534, checked in QuickGO on UniProtKB:E9QAG8). UniProtKB:Q96RE9 · human ZNF300 SUPPORTS TRANSFER Seed inspected: identity verified against live UniProt; an experimentally characterized nuclear transcription factor (IDA GO:0001228/GO:0045944, PMID:20585888). Supporting Evidence: UniProtKB:A0A8J0SCI2 SUBCELLULAR LOCATION: Nucleus {ECO:0000256\|ARBA:ARBA00004123}. file:XENTR/A0A8J0SCI2/A0A8J0SCI2-deep-research-falcon.md LOC101732730 is predicted to be a nuclear protein. C2H2 zinc finger transcription factors function by binding to genomic DNA regulatory elements (enhancers, promoters, silencers) and modulating chromatin structure and transcriptional activity
GO:0006357 regulation of transcription by RNA polymerase II	IBA GO_REF:0000033	ACCEPT	Summary: Regulation of transcription by RNA polymerase II is a reasonable biological process annotation for a Krueppel-type C2H2 zinc finger protein. The protein belongs to the Krueppel C2H2-type zinc finger family, whose members characteristically function as sequence-specific DNA-binding transcription factors regulating Pol II-dependent gene expression. UniProt notes it "may be involved in transcriptional regulation." The IBA annotation is phylogenetically inferred from characterized mammalian C2H2 zinc finger protein orthologs (mouse, rat, and human donors listed in the WITH/FROM field, within PANTHER family PTHR24381). Reason: Involvement in regulation of Pol II transcription is the core biological process for Krueppel-type C2H2 zinc finger transcription factors. The domain architecture (8 tandem C2H2 zinc fingers) and family classification strongly support this annotation. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN001225435 · PTHR24381 Eumetazoa-level PAINT node (IBD for GO:0006357) SUPPORTS TRANSFER Node assessed SOUND in interpro/panther/PTHR24381/PTHR24381-review.yaml (paint snapshot 20260529). The 13 seeds include experimentally verified positive AND negative regulators, so the direction-neutral parent GO:0006357 is the correct generalization at the node; directional child terms are correctly confined to narrower nodes elsewhere in the family. Appropriate for the target, whose regulatory direction cannot be inferred from its effector-less tandem zinc-finger architecture. UniProtKB:Q96RE9 · human ZNF300 SUPPORTS TRANSFER Seed inspected: IDA GO:0045944 (positive regulation; PMID:20585888, checked in QuickGO), a descendant of the propagated term. UniProtKB:P52738 · human ZNF140 SUPPORTS TRANSFER Seed inspected: IDA GO:0000122 (negative regulation; PMID:11470777, PMID:7649249, checked in QuickGO), a descendant of the propagated term on the opposite regulatory sign from ZNF300 - which is why the direction-neutral parent sits at the node. MGI:MGI:1916754 · mouse Zfp932 SUPPORTS TRANSFER Seed inspected: IDA GO:0000122 (PMID:21177534, PMID:22391310, checked in QuickGO on UniProtKB:E9QAG8). Supporting Evidence: UniProtKB:A0A8J0SCI2 FUNCTION: May be involved in transcriptional regulation. {ECO:0000256\|ARBA:ARBA00003767}. file:XENTR/A0A8J0SCI2/A0A8J0SCI2-deep-research-falcon.md As a transcription factor, LOC101732730 is not an enzyme with catalytic activity nor a transporter with substrate specificity. Instead, its primary function is to regulate gene expression through DNA binding and recruitment of transcriptional co-regulators
GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific	IBA GO_REF:0000033	ACCEPT	Summary: DNA-binding transcription factor activity (Pol II-specific) is the expected molecular function for a Krueppel-type C2H2 zinc finger protein. The protein has 8 tandem C2H2 zinc finger domains that mediate sequence-specific DNA binding, and belongs to the Krueppel C2H2-type zinc finger protein family. UniProt keywords include DNA-binding, Transcription, and Transcription regulation. Whether this protein acts as an activator or repressor (or both, context-dependently) is unknown, so the parent term GO:0000981 (which is agnostic to activator/repressor) is the appropriate level of specificity. Notably, ProtNLM2 predicted the child term GO:0001228 (transcription activator activity), but this was assessed as incorrect (NPI) because the protein is an effector-less tandem zinc finger array (no KRAB, SCAN, BTB, or identifiable activation domain), so activator-versus-repressor directionality cannot be inferred from sequence. Reason: This is the core molecular function annotation for Krueppel-type C2H2 zinc finger transcription factors. The domain architecture and family classification strongly support this term at the appropriate level of specificity. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN001225435 · PTHR24381 Eumetazoa-level PAINT node (IBD for GO:0000981) SUPPORTS TRANSFER Node assessed SOUND in interpro/panther/PTHR24381/PTHR24381-review.yaml (paint snapshot 20250904). The five seeds are experimentally characterized TFs spanning both regulatory directions (ZNF300 activator, ZNF140 repressor), so the PAINT curator's placement of the direction-agnostic parent GO:0000981 - rather than either directional child - is exactly right for descent onto effector-less members like the target, and matches this review's own NPI verdict on ProtNLM2's GO:0001228 activator prediction. UniProtKB:Q96RE9 · human ZNF300 SUPPORTS TRANSFER Seed inspected: IDA GO:0001228 (PMID:20585888, checked in QuickGO), a child of the propagated term. UniProtKB:P52738 · human ZNF140 SUPPORTS TRANSFER Seed inspected: IDA GO:0001227 (PMID:11470777, checked in QuickGO), the opposite-direction child of the propagated term. MGI:MGI:108094 · mouse Zfp92 SUPPORTS TRANSFER Seed identity verified via Alliance/mygene.info (zinc finger protein 92); its individual experimental annotations were not separately inspected. Supporting Evidence: UniProtKB:A0A8J0SCI2 FUNCTION: May be involved in transcriptional regulation. {ECO:0000256\|ARBA:ARBA00003767}. file:XENTR/A0A8J0SCI2/A0A8J0SCI2-deep-research-falcon.md Based on domain architecture, LOC101732730 is predicted to function as a sequence-specific DNA-binding transcription factor. The protein contains C2H2 zinc finger domains (PF00096), which are the hallmark of this function
GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA GO_REF:0000033	KEEP AS NON CORE	Summary: Sequence-specific DNA binding at Pol II cis-regulatory regions is well-supported by the presence of 8 tandem C2H2 zinc finger domains. Each zinc finger module uses residues at canonical positions to make base-specific contacts in the DNA major groove, enabling sequence-specific recognition of regulatory DNA elements. This term captures the DNA-binding component of the transcription factor activity and is appropriately annotated alongside GO:0000981. Reason: Sequence-specific DNA binding is mechanistically correct for a multi-zinc-finger protein, but it describes the binding activity component rather than the complete transcription factor function captured by GO:0000981. Retained as supporting context. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: PANTHER:PTN001225435 · PTHR24381 Eumetazoa-level PAINT node (IBD for GO:0000978) SUPPORTS TRANSFER Node assessed SOUND in interpro/panther/PTHR24381/PTHR24381-review.yaml (paint snapshot 20200709). A single-seed IBD, which is not weak support: the placement judgment is phylogenetic, and the one documented loss of cis-regulatory-region binding in this family (the ZNF445 clade) was pruned by PAINT with an IRD at its own node (PTN008615686) and does not implicate this one. Kept as non-core only because the binding term is the mechanistic component of the core TF activity GO:0000981, not because the propagation is doubted. MGI:MGI:1916754 · mouse Zfp932 SUPPORTS TRANSFER The sole IBD seed, inspected: identity verified via Alliance/mygene.info; IDA GO:0000978 from two independent papers (PMID:21177534, PMID:22391310, checked in QuickGO on UniProtKB:E9QAG8). Supporting Evidence: UniProtKB:A0A8J0SCI2 Belongs to the krueppel C2H2-type zinc-finger protein family. {ECO:0000256\|ARBA:ARBA00006991}. file:XENTR/A0A8J0SCI2/A0A8J0SCI2-deep-research-falcon.md C2H2 zinc finger proteins bind DNA through a well-characterized mechanism. Each zinc finger module is stabilized by coordination of a zinc ion (Zn²⁺) by two cysteine residues and two histidine residues, forming a compact finger-like structure
GO:0005634 nucleus	IEA GO_REF:0000044	ACCEPT	Summary: This is a second nucleus annotation derived from UniProt subcellular location vocabulary mapping (IEA via GO_REF:0000044), complementing the IBA annotation above. Nuclear localization is well-supported for this C2H2 zinc finger transcription factor. The "located_in" qualifier is less informative than the IBA "is_active_in" qualifier, but the annotation itself is correct. Reason: Correct annotation for nuclear localization of a C2H2 zinc finger transcription factor. Consistent with the IBA nucleus annotation and independently supported by UniProt ARBA subcellular location prediction. Supporting Evidence: UniProtKB:A0A8J0SCI2 SUBCELLULAR LOCATION: Nucleus {ECO:0000256\|ARBA:ARBA00004123}.

GO Term

Evidence

Action

Reason

IBA
GO_REF:0000033

Summary: Nuclear localization is well-supported for a C2H2 zinc finger transcription factor. The protein contains 8 tandem C2H2 zinc finger domains that mediate sequence-specific DNA binding, which requires nuclear localization. UniProt ARBA annotation also predicts nuclear subcellular location. The IBA annotation is based on PANTHER phylogenetic inference from numerous characterized nuclear C2H2-ZNF orthologs across vertebrates. The qualifier "is_active_in" (meaning it carries out its function in the nucleus) is appropriate for a transcription factor.

Reason: Nuclear localization is the expected and strongly supported location for a C2H2 zinc finger transcription factor. The phylogenetic inference from multiple well-characterized nuclear zinc finger protein orthologs provides robust support.

Propagation Review

Root cause: NO FAILURE CORE

Sources checked:

PANTHER:PTN001225435 · PTHR24381 Eumetazoa-level PAINT node (IBD for nucleus) SUPPORTS TRANSFER

Node assessed SOUND in interpro/panther/PTHR24381/PTHR24381-review.yaml (paint snapshot 20260529): the IBD is seeded by 39 experimentally annotated eumetazoan members, nuclear localization follows from the family's conserved DNA-binding architecture, and no diverged non-nuclear branch is documented anywhere in the cached family data.

MGI:MGI:1916754 · mouse Zfp932 SUPPORTS TRANSFER

Seed inspected: identity verified via Alliance/mygene.info; carries IDA GO:0005634 (PMID:21177534, checked in QuickGO on UniProtKB:E9QAG8).

UniProtKB:Q96RE9 · human ZNF300 SUPPORTS TRANSFER

Seed inspected: identity verified against live UniProt; an experimentally characterized nuclear transcription factor (IDA GO:0001228/GO:0045944, PMID:20585888).

Supporting Evidence:

UniProtKB:A0A8J0SCI2

SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.

file:XENTR/A0A8J0SCI2/A0A8J0SCI2-deep-research-falcon.md

LOC101732730 is predicted to be a nuclear protein. C2H2 zinc finger transcription factors function by binding to genomic DNA regulatory elements (enhancers, promoters, silencers) and modulating chromatin structure and transcriptional activity

GO:0006357 regulation of transcription by RNA polymerase II

IBA
GO_REF:0000033

Summary: Regulation of transcription by RNA polymerase II is a reasonable biological process annotation for a Krueppel-type C2H2 zinc finger protein. The protein belongs to the Krueppel C2H2-type zinc finger family, whose members characteristically function as sequence-specific DNA-binding transcription factors regulating Pol II-dependent gene expression. UniProt notes it "may be involved in transcriptional regulation." The IBA annotation is phylogenetically inferred from characterized mammalian C2H2 zinc finger protein orthologs (mouse, rat, and human donors listed in the WITH/FROM field, within PANTHER family PTHR24381).

Reason: Involvement in regulation of Pol II transcription is the core biological process for Krueppel-type C2H2 zinc finger transcription factors. The domain architecture (8 tandem C2H2 zinc fingers) and family classification strongly support this annotation.

Propagation Review

Root cause: NO FAILURE CORE

Sources checked:

PANTHER:PTN001225435 · PTHR24381 Eumetazoa-level PAINT node (IBD for GO:0006357) SUPPORTS TRANSFER

Node assessed SOUND in interpro/panther/PTHR24381/PTHR24381-review.yaml (paint snapshot 20260529). The 13 seeds include experimentally verified positive AND negative regulators, so the direction-neutral parent GO:0006357 is the correct generalization at the node; directional child terms are correctly confined to narrower nodes elsewhere in the family. Appropriate for the target, whose regulatory direction cannot be inferred from its effector-less tandem zinc-finger architecture.

UniProtKB:Q96RE9 · human ZNF300 SUPPORTS TRANSFER

Seed inspected: IDA GO:0045944 (positive regulation; PMID:20585888, checked in QuickGO), a descendant of the propagated term.

UniProtKB:P52738 · human ZNF140 SUPPORTS TRANSFER

Seed inspected: IDA GO:0000122 (negative regulation; PMID:11470777, PMID:7649249, checked in QuickGO), a descendant of the propagated term on the opposite regulatory sign from ZNF300 - which is why the direction-neutral parent sits at the node.

MGI:MGI:1916754 · mouse Zfp932 SUPPORTS TRANSFER

Seed inspected: IDA GO:0000122 (PMID:21177534, PMID:22391310, checked in QuickGO on UniProtKB:E9QAG8).

Supporting Evidence:

UniProtKB:A0A8J0SCI2

FUNCTION: May be involved in transcriptional regulation. {ECO:0000256|ARBA:ARBA00003767}.

file:XENTR/A0A8J0SCI2/A0A8J0SCI2-deep-research-falcon.md

As a transcription factor, LOC101732730 is not an enzyme with catalytic activity nor a transporter with substrate specificity. Instead, its primary function is to regulate gene expression through DNA binding and recruitment of transcriptional co-regulators

GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific

IBA
GO_REF:0000033

Summary: DNA-binding transcription factor activity (Pol II-specific) is the expected molecular function for a Krueppel-type C2H2 zinc finger protein. The protein has 8 tandem C2H2 zinc finger domains that mediate sequence-specific DNA binding, and belongs to the Krueppel C2H2-type zinc finger protein family. UniProt keywords include DNA-binding, Transcription, and Transcription regulation. Whether this protein acts as an activator or repressor (or both, context-dependently) is unknown, so the parent term GO:0000981 (which is agnostic to activator/repressor) is the appropriate level of specificity. Notably, ProtNLM2 predicted the child term GO:0001228 (transcription activator activity), but this was assessed as incorrect (NPI) because the protein is an effector-less tandem zinc finger array (no KRAB, SCAN, BTB, or identifiable activation domain), so activator-versus-repressor directionality cannot be inferred from sequence.

Reason: This is the core molecular function annotation for Krueppel-type C2H2 zinc finger transcription factors. The domain architecture and family classification strongly support this term at the appropriate level of specificity.

Propagation Review

Root cause: NO FAILURE CORE

Sources checked:

PANTHER:PTN001225435 · PTHR24381 Eumetazoa-level PAINT node (IBD for GO:0000981) SUPPORTS TRANSFER

Node assessed SOUND in interpro/panther/PTHR24381/PTHR24381-review.yaml (paint snapshot 20250904). The five seeds are experimentally characterized TFs spanning both regulatory directions (ZNF300 activator, ZNF140 repressor), so the PAINT curator's placement of the direction-agnostic parent GO:0000981 - rather than either directional child - is exactly right for descent onto effector-less members like the target, and matches this review's own NPI verdict on ProtNLM2's GO:0001228 activator prediction.

UniProtKB:Q96RE9 · human ZNF300 SUPPORTS TRANSFER

Seed inspected: IDA GO:0001228 (PMID:20585888, checked in QuickGO), a child of the propagated term.

UniProtKB:P52738 · human ZNF140 SUPPORTS TRANSFER

Seed inspected: IDA GO:0001227 (PMID:11470777, checked in QuickGO), the opposite-direction child of the propagated term.

MGI:MGI:108094 · mouse Zfp92 SUPPORTS TRANSFER

Seed identity verified via Alliance/mygene.info (zinc finger protein 92); its individual experimental annotations were not separately inspected.

Supporting Evidence:

UniProtKB:A0A8J0SCI2

FUNCTION: May be involved in transcriptional regulation. {ECO:0000256|ARBA:ARBA00003767}.

file:XENTR/A0A8J0SCI2/A0A8J0SCI2-deep-research-falcon.md

Based on domain architecture, LOC101732730 is predicted to function as a sequence-specific DNA-binding transcription factor. The protein contains C2H2 zinc finger domains (PF00096), which are the hallmark of this function

GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding

IBA
GO_REF:0000033

KEEP AS NON CORE

Summary: Sequence-specific DNA binding at Pol II cis-regulatory regions is well-supported by the presence of 8 tandem C2H2 zinc finger domains. Each zinc finger module uses residues at canonical positions to make base-specific contacts in the DNA major groove, enabling sequence-specific recognition of regulatory DNA elements. This term captures the DNA-binding component of the transcription factor activity and is appropriately annotated alongside GO:0000981.

Reason: Sequence-specific DNA binding is mechanistically correct for a multi-zinc-finger protein, but it describes the binding activity component rather than the complete transcription factor function captured by GO:0000981. Retained as supporting context.

Propagation Review

Root cause: NO FAILURE NON CORE

Sources checked:

PANTHER:PTN001225435 · PTHR24381 Eumetazoa-level PAINT node (IBD for GO:0000978) SUPPORTS TRANSFER

Node assessed SOUND in interpro/panther/PTHR24381/PTHR24381-review.yaml (paint snapshot 20200709). A single-seed IBD, which is not weak support: the placement judgment is phylogenetic, and the one documented loss of cis-regulatory-region binding in this family (the ZNF445 clade) was pruned by PAINT with an IRD at its own node (PTN008615686) and does not implicate this one. Kept as non-core only because the binding term is the mechanistic component of the core TF activity GO:0000981, not because the propagation is doubted.

MGI:MGI:1916754 · mouse Zfp932 SUPPORTS TRANSFER

The sole IBD seed, inspected: identity verified via Alliance/mygene.info; IDA GO:0000978 from two independent papers (PMID:21177534, PMID:22391310, checked in QuickGO on UniProtKB:E9QAG8).

Supporting Evidence:

UniProtKB:A0A8J0SCI2

Belongs to the krueppel C2H2-type zinc-finger protein family. {ECO:0000256|ARBA:ARBA00006991}.

file:XENTR/A0A8J0SCI2/A0A8J0SCI2-deep-research-falcon.md

C2H2 zinc finger proteins bind DNA through a well-characterized mechanism. Each zinc finger module is stabilized by coordination of a zinc ion (Zn²⁺) by two cysteine residues and two histidine residues, forming a compact finger-like structure

GO:0005634 nucleus

IEA
GO_REF:0000044

Summary: This is a second nucleus annotation derived from UniProt subcellular location vocabulary mapping (IEA via GO_REF:0000044), complementing the IBA annotation above. Nuclear localization is well-supported for this C2H2 zinc finger transcription factor. The "located_in" qualifier is less informative than the IBA "is_active_in" qualifier, but the annotation itself is correct.

Reason: Correct annotation for nuclear localization of a C2H2 zinc finger transcription factor. Consistent with the IBA nucleus annotation and independently supported by UniProt ARBA subcellular location prediction.

Supporting Evidence:

UniProtKB:A0A8J0SCI2

SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.

Core Functions

LOC101732730 is predicted to function as a sequence-specific DNA-binding transcription factor that regulates RNA polymerase II-dependent transcription from the nucleus. The protein contains 8 tandem C2H2 zinc finger domains that mediate sequence-specific DNA recognition. Whether it acts as a transcriptional activator or repressor is unknown. By analogy to related Krueppel-like zinc finger proteins in Xenopus, it may regulate gene expression programs during early embryonic development (gastrulation).

Molecular Function:

DNA-binding transcription factor activity, RNA polymerase II-specific

Directly Involved In:

regulation of transcription by RNA polymerase II

Cellular Locations:

nucleus

Supporting Evidence:

UniProtKB:A0A8J0SCI2
FUNCTION: May be involved in transcriptional regulation. {ECO:0000256|ARBA:ARBA00003767}.
file:XENTR/A0A8J0SCI2/A0A8J0SCI2-deep-research-falcon.md
Based on domain architecture, LOC101732730 is predicted to function as a sequence-specific DNA-binding transcription factor. The protein contains C2H2 zinc finger domains (PF00096), which are the hallmark of this function

External Prediction Reviews

These computational predictions are reviewed separately from the GOA annotation set used for this review. The assessments below are from this project and do not constitute official GO annotations or endorsement by GO/UniProt. They are not included in the existing annotation review above.

ProtNLM2 External predictions

View prediction review YAML · A0A8J0SCI2-protnlm-predictions-review.yaml · Review status: COMPLETE

The C2H2 zinc-finger architecture supports the existing curated transcription-factor and nuclear annotations. Whether this factor specifically activates transcription is unresolved.

Source documents: genes/XENTR/A0A8J0SCI2/A0A8J0SCI2-uniprot.txt · genes/XENTR/A0A8J0SCI2/A0A8J0SCI2-goa.tsv · genes/XENTR/A0A8J0SCI2/A0A8J0SCI2-hypotheses/prediction-tf-activator/openscientist.md

Review score: 2 = concordant with evidence; 1 = uncertain; 0 = discordant with evidence. This is an assessment score, not a model probability.

GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding GO_MF

CNN — Correct but not novel Review score: 2/2

Prediction method: ProtNLM2 · Version: UniProt 2024_06 pilot

Review rationale: The target contains eight tandem C2H2 zinc-finger domains, consistent with sequence-specific DNA recognition. The cached GOA record has an exact curated IBA for RNA polymerase II cis-regulatory region sequence-specific DNA binding, alongside the corresponding transcription-factor activity. This is a curator-assessed phylogenetic inference consistent with the target architecture, not an ARBA assertion. The available evidence supports the function without identifying a particular DNA motif or target gene.

Supporting Evidence:

file:XENTR/A0A8J0SCI2/A0A8J0SCI2-uniprot.txt: "ID A0A8J0SCI2_XENTR Unreviewed; 265 AA. ... DR GO; GO:0005634; C:nucleus; IBA:GO_Central. ... DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central. ... DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central. ... DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central. ... DR InterPro; IPR013087; Znf_C2H2_type. ... DR PANTHER; PTHR24381:SF440; OOCYTE ZINC FINGER PROTEIN XLCOF7.1-LIKE; 1. ... FT DOMAIN 37..64 ... FT /note="C2H2-type" ... FT DOMAIN 65..92 ... FT /note="C2H2-type" ... FT DOMAIN 93..120 ... FT /note="C2H2-type" ... FT DOMAIN 121..148 ... FT /note="C2H2-type" ... FT DOMAIN 149..176 ... FT /note="C2H2-type" ... FT DOMAIN 177..204 ... FT /note="C2H2-type" ... FT DOMAIN 205..232 ... FT /note="C2H2-type" ... FT DOMAIN 233..260 ... FT /note="C2H2-type""

GO:0006357 regulation of transcription by RNA polymerase II GO_BP

CNN — Correct but not novel Review score: 2/2

Prediction method: ProtNLM2 · Version: UniProt 2024_06 pilot

Review rationale: The target has the tandem C2H2 zinc-finger architecture of a sequence-specific DNA-binding factor. The exact regulation of transcription by RNA polymerase II annotation is present as a curated IBA in the cached GOA record, together with compatible DNA-binding transcription-factor activity. These phylogenetic annotations and the target architecture support the broad regulatory process. They do not specify whether the factor activates or represses its targets.

Supporting Evidence:

file:XENTR/A0A8J0SCI2/A0A8J0SCI2-uniprot.txt: "ID A0A8J0SCI2_XENTR Unreviewed; 265 AA. ... DR GO; GO:0005634; C:nucleus; IBA:GO_Central. ... DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central. ... DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central. ... DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central. ... DR InterPro; IPR013087; Znf_C2H2_type. ... DR PANTHER; PTHR24381:SF440; OOCYTE ZINC FINGER PROTEIN XLCOF7.1-LIKE; 1. ... FT DOMAIN 37..64 ... FT /note="C2H2-type" ... FT DOMAIN 65..92 ... FT /note="C2H2-type" ... FT DOMAIN 93..120 ... FT /note="C2H2-type" ... FT DOMAIN 121..148 ... FT /note="C2H2-type" ... FT DOMAIN 149..176 ... FT /note="C2H2-type" ... FT DOMAIN 177..204 ... FT /note="C2H2-type" ... FT DOMAIN 205..232 ... FT /note="C2H2-type" ... FT DOMAIN 233..260 ... FT /note="C2H2-type""

GO:0005634 nucleus GO_CC

CNN — Correct but not novel Review score: 2/2

Prediction method: ProtNLM2 · Version: UniProt 2024_06 pilot

Review rationale: The target zinc-finger architecture is compatible with its curated DNA-binding transcription-factor assignment. Nucleus is already present as a curated IBA in the cached GOA record, supporting this localization through a phylogenetic judgment consistent with the molecular function. No target localization experiment is asserted. The exact overlap makes this a supported, non-novel annotation.

Supporting Evidence:

file:XENTR/A0A8J0SCI2/A0A8J0SCI2-uniprot.txt: "ID A0A8J0SCI2_XENTR Unreviewed; 265 AA. ... DR GO; GO:0005634; C:nucleus; IBA:GO_Central. ... DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central. ... DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central. ... DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central. ... DR InterPro; IPR013087; Znf_C2H2_type. ... DR PANTHER; PTHR24381:SF440; OOCYTE ZINC FINGER PROTEIN XLCOF7.1-LIKE; 1. ... FT DOMAIN 37..64 ... FT /note="C2H2-type" ... FT DOMAIN 65..92 ... FT /note="C2H2-type" ... FT DOMAIN 93..120 ... FT /note="C2H2-type" ... FT DOMAIN 121..148 ... FT /note="C2H2-type" ... FT DOMAIN 149..176 ... FT /note="C2H2-type" ... FT DOMAIN 177..204 ... FT /note="C2H2-type" ... FT DOMAIN 205..232 ... FT /note="C2H2-type" ... FT DOMAIN 233..260 ... FT /note="C2H2-type""

GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific GO_MF

UNC — Uncertain Review score: 1/2

Prediction method: ProtNLM2 · Version: UniProt 2024_06 pilot

Review rationale: The zinc-finger array and existing curated IBA support a DNA-binding RNA polymerase II transcription factor. They do not determine whether its effect on transcription is activating, repressing, or context-dependent. Absence of a recognizable activation domain is not decisive because activation can depend on partners or short interaction regions. The activator-specific term is absent from the cached annotations, and evidence for regulatory direction is needed.

Supporting Evidence:

file:XENTR/A0A8J0SCI2/A0A8J0SCI2-uniprot.txt: "ID A0A8J0SCI2_XENTR Unreviewed; 265 AA. ... DR GO; GO:0005634; C:nucleus; IBA:GO_Central. ... DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central. ... DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central. ... DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central. ... DR InterPro; IPR013087; Znf_C2H2_type. ... DR PANTHER; PTHR24381:SF440; OOCYTE ZINC FINGER PROTEIN XLCOF7.1-LIKE; 1. ... FT DOMAIN 37..64 ... FT /note="C2H2-type" ... FT DOMAIN 65..92 ... FT /note="C2H2-type" ... FT DOMAIN 93..120 ... FT /note="C2H2-type" ... FT DOMAIN 121..148 ... FT /note="C2H2-type" ... FT DOMAIN 149..176 ... FT /note="C2H2-type" ... FT DOMAIN 177..204 ... FT /note="C2H2-type" ... FT DOMAIN 205..232 ... FT /note="C2H2-type" ... FT DOMAIN 233..260 ... FT /note="C2H2-type""
file:XENTR/A0A8J0SCI2/A0A8J0SCI2-hypotheses/prediction-tf-activator/openscientist.md: "it is simply not demonstrably an *activator*"

Deep Research

Falcon

(A0A8J0SCI2-deep-research-falcon.md)

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OpenScientist

(A0A8J0SCI2-hypotheses/prediction-tf-activator/openscientist.md)

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Gene Description

Existing Annotations Review

Core Functions

References

External Prediction Reviews

ProtNLM2 External predictions

Deep Research

Falcon

OpenScientist

📚 Additional Documentation

Notes

📄 View Raw YAML