AGO1

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0005634 nucleus	IBA GO_REF:0000033	KEEP AS NON CORE	Summary: nucleus is a plausible or reported location/context but is not the core functional site annotation for AGO1. Reason: The core location is cytoplasmic RISC/RNP granules; nuclear, membrane, synaptic, exosomal, or other compartment annotations should remain non-core unless tied to a specific curated mechanism. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005737 cytoplasm	IBA GO_REF:0000033	ACCEPT	Summary: cytoplasm is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0035194 regulatory ncRNA-mediated post-transcriptional gene silencing	IBA GO_REF:0000033	ACCEPT	Summary: regulatory ncRNA-mediated post-transcriptional gene silencing is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0016442 RISC complex	IBA GO_REF:0000033	ACCEPT	Summary: RISC complex is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0036464 cytoplasmic ribonucleoprotein granule	IBA GO_REF:0000033	ACCEPT	Summary: cytoplasmic ribonucleoprotein granule is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0031054 pre-miRNA processing	IBA GO_REF:0000033	MARK AS OVER ANNOTATED	Summary: pre-miRNA processing overstates the direct role of AGO1 in canonical miRNA biogenesis. Reason: The better-supported role is as a loaded Argonaute effector after small-RNA processing, not as the Drosha/Dicer processing enzyme for pre-miRNAs. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0035198 miRNA binding	IBA GO_REF:0000033	ACCEPT	Summary: miRNA binding is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0003727 single-stranded RNA binding	IBA GO_REF:0000033	MARK AS OVER ANNOTATED	Summary: single-stranded RNA binding is too generic for AGO1. Reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC rather than a broad nucleic-acid or RNA-binding label. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0000932 P-body	IEA GO_REF:0000120	ACCEPT	Summary: P-body is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0003676 nucleic acid binding	IEA GO_REF:0000002	MARK AS OVER ANNOTATED	Summary: nucleic acid binding is too generic for AGO1. Reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC rather than a broad nucleic-acid or RNA-binding label. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0003723 RNA binding	IEA GO_REF:0000120	MARK AS OVER ANNOTATED	Summary: RNA binding is too generic for AGO1. Reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC rather than a broad nucleic-acid or RNA-binding label. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0003725 double-stranded RNA binding	IEA GO_REF:0000117	MARK AS OVER ANNOTATED	Summary: double-stranded RNA binding is too generic for AGO1. Reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC rather than a broad nucleic-acid or RNA-binding label. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0003727 single-stranded RNA binding	IEA GO_REF:0000117	MARK AS OVER ANNOTATED	Summary: single-stranded RNA binding is too generic for AGO1. Reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC rather than a broad nucleic-acid or RNA-binding label. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0031054 pre-miRNA processing	IEA GO_REF:0000117	MARK AS OVER ANNOTATED	Summary: pre-miRNA processing overstates the direct role of AGO1 in canonical miRNA biogenesis. Reason: The better-supported role is as a loaded Argonaute effector after small-RNA processing, not as the Drosha/Dicer processing enzyme for pre-miRNAs. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0035278 miRNA-mediated gene silencing by inhibition of translation	IEA GO_REF:0000117	ACCEPT	Summary: miRNA-mediated gene silencing by inhibition of translation is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0036464 cytoplasmic ribonucleoprotein granule	IEA GO_REF:0000117	ACCEPT	Summary: cytoplasmic ribonucleoprotein granule is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0070578 RISC-loading complex	IEA GO_REF:0000117	ACCEPT	Summary: RISC-loading complex is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0070922 RISC complex assembly	IEA GO_REF:0000117	ACCEPT	Summary: RISC complex assembly is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005515 protein binding	IPI PMID:12526743 Short-interfering-RNA-mediated gene silencing in mammalian c...	MARK AS OVER ANNOTATED	Summary: Generic protein binding does not capture the specific Argonaute/RISC function. Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005515 protein binding	IPI PMID:16756390 Translation repression in human cells by microRNA-induced ge...	MARK AS OVER ANNOTATED	Summary: Generic protein binding does not capture the specific Argonaute/RISC function. Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005515 protein binding	IPI PMID:17891150 A conserved motif in Argonaute-interacting proteins mediates...	MARK AS OVER ANNOTATED	Summary: Generic protein binding does not capture the specific Argonaute/RISC function. Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005515 protein binding	IPI PMID:17932509 Proteomic and functional analysis of Argonaute-containing mR...	MARK AS OVER ANNOTATED	Summary: Generic protein binding does not capture the specific Argonaute/RISC function. Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005515 protein binding	IPI PMID:18482256 Protein microarray analysis identifies human cellular prion ...	MARK AS OVER ANNOTATED	Summary: Generic protein binding does not capture the specific Argonaute/RISC function. Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005515 protein binding	IPI PMID:19167051 Importin 8 is a gene silencing factor that targets argonaute...	MARK AS OVER ANNOTATED	Summary: Generic protein binding does not capture the specific Argonaute/RISC function. Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005515 protein binding	IPI PMID:19324964 The C-terminal half of human Ago2 binds to multiple GW-rich ...	MARK AS OVER ANNOTATED	Summary: Generic protein binding does not capture the specific Argonaute/RISC function. Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005515 protein binding	IPI PMID:19383768 The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C s...	MARK AS OVER ANNOTATED	Summary: Generic protein binding does not capture the specific Argonaute/RISC function. Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005515 protein binding	IPI PMID:19716330 Mammalian miRNA RISC recruits CAF1 and PABP to affect PABP-d...	MARK AS OVER ANNOTATED	Summary: Generic protein binding does not capture the specific Argonaute/RISC function. Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005515 protein binding	IPI PMID:22484317 Human prion protein binds Argonaute and promotes accumulatio...	MARK AS OVER ANNOTATED	Summary: Generic protein binding does not capture the specific Argonaute/RISC function. Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005515 protein binding	IPI PMID:25036637 A quantitative chaperone interaction network reveals the arc...	MARK AS OVER ANNOTATED	Summary: Generic protein binding does not capture the specific Argonaute/RISC function. Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005515 protein binding	IPI PMID:28330616 Systematic Analysis of Human Protein Phosphatase Interaction...	MARK AS OVER ANNOTATED	Summary: Generic protein binding does not capture the specific Argonaute/RISC function. Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005515 protein binding	IPI PMID:28683311 Argonaute Utilization for miRNA Silencing Is Determined by P...	MARK AS OVER ANNOTATED	Summary: Generic protein binding does not capture the specific Argonaute/RISC function. Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005515 protein binding	IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling...	MARK AS OVER ANNOTATED	Summary: Generic protein binding does not capture the specific Argonaute/RISC function. Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005515 protein binding	IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce...	MARK AS OVER ANNOTATED	Summary: Generic protein binding does not capture the specific Argonaute/RISC function. Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005515 protein binding	IPI PMID:35709258 Spatial centrosome proteome of human neural cells uncovers d...	MARK AS OVER ANNOTATED	Summary: Generic protein binding does not capture the specific Argonaute/RISC function. Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005515 protein binding	IPI PMID:40205054 Multimodal cell maps as a foundation for structural and func...	MARK AS OVER ANNOTATED	Summary: Generic protein binding does not capture the specific Argonaute/RISC function. Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0000976 transcription cis-regulatory region binding	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: transcription cis-regulatory region binding reflects a reported nuclear or regulatory context rather than the main conserved AGO1 function. Reason: Argonaute paralogs have reported nuclear/regulatory interactions, but the dominant conserved function remains small-RNA-guided post-transcriptional repression. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: RNA polymerase II cis-regulatory region sequence-specific DNA binding reflects a reported nuclear or regulatory context rather than the main conserved AGO1 function. Reason: Argonaute paralogs have reported nuclear/regulatory interactions, but the dominant conserved function remains small-RNA-guided post-transcriptional repression. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005634 nucleus	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: nucleus is a plausible or reported location/context but is not the core functional site annotation for AGO1. Reason: The core location is cytoplasmic RISC/RNP granules; nuclear, membrane, synaptic, exosomal, or other compartment annotations should remain non-core unless tied to a specific curated mechanism. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	IEA GO_REF:0000107	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0016442 RISC complex	IEA GO_REF:0000120	ACCEPT	Summary: RISC complex is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0035198 miRNA binding	IEA GO_REF:0000120	ACCEPT	Summary: miRNA binding is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:1901224 positive regulation of non-canonical NF-kappaB signal transduction	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: positive regulation of non-canonical NF-kappaB signal transduction reflects a reported nuclear or regulatory context rather than the main conserved AGO1 function. Reason: Argonaute paralogs have reported nuclear/regulatory interactions, but the dominant conserved function remains small-RNA-guided post-transcriptional repression. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0035196 miRNA processing	NAS PMID:28781232 Multivalent Recruitment of Human Argonaute by GW182.	MARK AS OVER ANNOTATED	Summary: miRNA processing overstates the direct role of AGO1 in canonical miRNA biogenesis. Reason: The better-supported role is as a loaded Argonaute effector after small-RNA processing, not as the Drosha/Dicer processing enzyme for pre-miRNAs. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0036464 cytoplasmic ribonucleoprotein granule	IDA GO_REF:0000052	ACCEPT	Summary: cytoplasmic ribonucleoprotein granule is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005737 cytoplasm	IDA PMID:17932509 Proteomic and functional analysis of Argonaute-containing mR...	ACCEPT	Summary: cytoplasm is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0016525 negative regulation of angiogenesis	IMP PMID:23426184 Hypoxia-responsive miRNAs target argonaute 1 to promote angi...	KEEP AS NON CORE	Summary: negative regulation of angiogenesis is downstream or context-specific for AGO1. Reason: This process may be supported in a particular experiment or pathway model, but it is peripheral to the core Argonaute/RISC molecular role. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0045944 positive regulation of transcription by RNA polymerase II	IMP PMID:25336585 Cellular microRNAs up-regulate transcription via interaction...	KEEP AS NON CORE	Summary: positive regulation of transcription by RNA polymerase II reflects a reported nuclear or regulatory context rather than the main conserved AGO1 function. Reason: Argonaute paralogs have reported nuclear/regulatory interactions, but the dominant conserved function remains small-RNA-guided post-transcriptional repression. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0001046 core promoter sequence-specific DNA binding	IMP PMID:25336585 Cellular microRNAs up-regulate transcription via interaction...	KEEP AS NON CORE	Summary: core promoter sequence-specific DNA binding reflects a reported nuclear or regulatory context rather than the main conserved AGO1 function. Reason: Argonaute paralogs have reported nuclear/regulatory interactions, but the dominant conserved function remains small-RNA-guided post-transcriptional repression. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005634 nucleus	IC PMID:25336585 Cellular microRNAs up-regulate transcription via interaction...	KEEP AS NON CORE	Summary: nucleus is a plausible or reported location/context but is not the core functional site annotation for AGO1. Reason: The core location is cytoplasmic RISC/RNP granules; nuclear, membrane, synaptic, exosomal, or other compartment annotations should remain non-core unless tied to a specific curated mechanism. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0000993 RNA polymerase II complex binding	IDA PMID:25336585 Cellular microRNAs up-regulate transcription via interaction...	KEEP AS NON CORE	Summary: RNA polymerase II complex binding reflects a reported nuclear or regulatory context rather than the main conserved AGO1 function. Reason: Argonaute paralogs have reported nuclear/regulatory interactions, but the dominant conserved function remains small-RNA-guided post-transcriptional repression. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005515 protein binding	IPI PMID:25336585 Cellular microRNAs up-regulate transcription via interaction...	MARK AS OVER ANNOTATED	Summary: Generic protein binding does not capture the specific Argonaute/RISC function. Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0035196 miRNA processing	IMP PMID:22795694 Slicing-independent RISC activation requires the argonaute P...	MARK AS OVER ANNOTATED	Summary: miRNA processing overstates the direct role of AGO1 in canonical miRNA biogenesis. Reason: The better-supported role is as a loaded Argonaute effector after small-RNA processing, not as the Drosha/Dicer processing enzyme for pre-miRNAs. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0031054 pre-miRNA processing	IDA PMID:19966796 ATP-dependent human RISC assembly pathways.	MARK AS OVER ANNOTATED	Summary: pre-miRNA processing overstates the direct role of AGO1 in canonical miRNA biogenesis. Reason: The better-supported role is as a loaded Argonaute effector after small-RNA processing, not as the Drosha/Dicer processing enzyme for pre-miRNAs. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0003725 double-stranded RNA binding	IDA PMID:19966796 ATP-dependent human RISC assembly pathways.	MARK AS OVER ANNOTATED	Summary: double-stranded RNA binding is too generic for AGO1. Reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC rather than a broad nucleic-acid or RNA-binding label. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0003727 single-stranded RNA binding	IDA PMID:19966796 ATP-dependent human RISC assembly pathways.	MARK AS OVER ANNOTATED	Summary: single-stranded RNA binding is too generic for AGO1. Reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC rather than a broad nucleic-acid or RNA-binding label. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0016442 RISC complex	IDA PMID:19966796 ATP-dependent human RISC assembly pathways.	ACCEPT	Summary: RISC complex is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0070578 RISC-loading complex	IDA PMID:19966796 ATP-dependent human RISC assembly pathways.	ACCEPT	Summary: RISC-loading complex is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0070922 RISC complex assembly	IDA PMID:19966796 ATP-dependent human RISC assembly pathways.	ACCEPT	Summary: RISC complex assembly is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0090625 siRNA-mediated gene silencing by mRNA destabilization	IDA NOT PMID:15260970 Human Argonaute2 mediates RNA cleavage targeted by miRNAs an...	ACCEPT	Summary: This NOT annotation is appropriate; AGO1 is not the Argonaute paralog that mediates siRNA-guided target mRNA cleavage/destabilization in this study. Reason: PMID:15260970 supports AGO1 association with small RNAs/RISC but shows that endonucleolytic target cleavage activity is associated with AGO2, not AGO1. UniProt also states that AGO1 lacks endonuclease activity and does not appear to cleave target mRNAs. Supporting Evidence: PMID:15260970 Purification of the FLAG/HA-epitope-tagged Ago containing complexes from different human cell lines revealed that endonuclease activity is exclusively associated with Ago2. file:human/AGO1/AGO1-uniprot.txt Lacks endonuclease activity and does not appear to cleave target mRNAs.
GO:0005737 cytoplasm	IDA PMID:15260970 Human Argonaute2 mediates RNA cleavage targeted by miRNAs an...	ACCEPT	Summary: cytoplasm is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0016442 RISC complex	IDA PMID:15260970 Human Argonaute2 mediates RNA cleavage targeted by miRNAs an...	ACCEPT	Summary: RISC complex is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0035198 miRNA binding	IDA PMID:15260970 Human Argonaute2 mediates RNA cleavage targeted by miRNAs an...	ACCEPT	Summary: miRNA binding is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0035279 miRNA-mediated gene silencing by mRNA destabilization	IDA NOT PMID:15260970 Human Argonaute2 mediates RNA cleavage targeted by miRNAs an...	ACCEPT	Summary: This NOT annotation is appropriate; PMID:15260970 does not support assigning AGO1 to AGO2-like miRNA-guided target mRNA cleavage/destabilization. Reason: AGO1 binds miRNAs and participates in miRISC, but the cited work and UniProt distinguish this non-slicer role from AGO2-dependent target RNA cleavage. The NOT annotation should be retained rather than converted into a positive AGO1 mRNA-destabilization function. Supporting Evidence: PMID:15260970 The specific role of Ago2 in guiding target RNA cleavage was confirmed independently by siRNA-based depletion of individual Ago members in combination with a sensitive positive-readout reporter assay. file:human/AGO1/AGO1-uniprot.txt Lacks endonuclease activity and does not appear to cleave target mRNAs.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-5578742	KEEP AS NON CORE	Summary: nucleoplasm is a plausible or reported location/context but is not the core functional site annotation for AGO1. Reason: The core location is cytoplasmic RISC/RNP granules; nuclear, membrane, synaptic, exosomal, or other compartment annotations should remain non-core unless tied to a specific curated mechanism. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0003723 RNA binding	IDA PMID:23809764 Eukaryote-specific insertion elements control human ARGONAUT...	MARK AS OVER ANNOTATED	Summary: RNA binding is too generic for AGO1. Reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC rather than a broad nucleic-acid or RNA-binding label. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0004521 RNA endonuclease activity	IDA NOT PMID:23809764 Eukaryote-specific insertion elements control human ARGONAUT...	ACCEPT	Summary: This NOT annotation is correct; wild-type AGO1 lacks RNA endonuclease/slicer activity. Reason: PMID:23809764 directly supports the negated annotation by showing structural constraints that keep wild-type AGO1 from placing a guide-target RNA duplex into the endonuclease active site. The NOT evidence should be retained. Supporting Evidence: PMID:23809764 We predicted that even upon restoration of the catalytic tetrad, hAGO1-cS7 sterically hinders the placement of a fully paired guide-target RNA duplex into the endonuclease active site.
GO:0003723 RNA binding	HDA PMID:22681889 The mRNA-bound proteome and its global occupancy profile on ...	MARK AS OVER ANNOTATED	Summary: RNA binding is too generic for AGO1. Reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC rather than a broad nucleic-acid or RNA-binding label. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-1606561	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-1606682	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-1912362	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-1912363	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-1912364	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-1912366	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-1912367	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-1912368	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-1912406	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-2318752	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-3209151	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-426489	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-426522	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-4518575	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-5687103	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-8935766	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-8937097	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-8937134	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-8938440	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-8938487	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-8938507	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-8944650	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-8944684	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-8944706	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-9011958	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-9012203	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-9012208	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-9038545	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-9618392	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-9618486	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-9624925	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-9657791	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-9858289	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-9858309	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-9924921	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-9925095	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-9925158	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-9925159	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005829 cytosol	TAS Reactome:R-HSA-9925324	ACCEPT	Summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005515 protein binding	IPI PMID:22915799 HIV-1 replication and APOBEC3 antiviral activity are not reg...	MARK AS OVER ANNOTATED	Summary: Generic protein binding does not capture the specific Argonaute/RISC function. Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005515 protein binding	IPI PMID:16289642 Identification of novel argonaute-associated proteins.	MARK AS OVER ANNOTATED	Summary: Generic protein binding does not capture the specific Argonaute/RISC function. Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005515 protein binding	IPI PMID:20616046 LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for...	MARK AS OVER ANNOTATED	Summary: Generic protein binding does not capture the specific Argonaute/RISC function. Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0005737 cytoplasm	IDA PMID:20014101 Mouse ZAR1-like (XM_359149) colocalizes with mRNA processing...	ACCEPT	Summary: cytoplasm is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0016442 RISC complex	IDA PMID:17932509 Proteomic and functional analysis of Argonaute-containing mR...	ACCEPT	Summary: RISC complex is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0000956 nuclear-transcribed mRNA catabolic process	IDA PMID:18771919 Importance of translation and nonnucleolytic ago proteins fo...	KEEP AS NON CORE	Summary: nuclear-transcribed mRNA catabolic process is downstream or context-specific for AGO1. Reason: This process may be supported in a particular experiment or pathway model, but it is peripheral to the core Argonaute/RISC molecular role. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.
GO:0035278 miRNA-mediated gene silencing by inhibition of translation	IDA PMID:18771919 Importance of translation and nonnucleolytic ago proteins fo...	ACCEPT	Summary: miRNA-mediated gene silencing by inhibition of translation is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector. Reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments. Supporting Evidence: file:human/AGO1/AGO1-deep-research-falcon.md Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways.

Core Functions

Small-RNA-guided post-transcriptional repression as a non-slicer Argonaute component of miRISC.

Molecular Function:

miRNA binding

Directly Involved In:

regulatory ncRNA-mediated post-transcriptional gene silencing miRNA-mediated gene silencing by inhibition of translation

Cellular Locations:

cytoplasm P-body

In Complex:

RISC complex

Supporting Evidence:

file:human/AGO1/AGO1-deep-research-falcon.md
Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay** through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.

References

GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000052

Gene Ontology annotation based on curation of immunofluorescence data

GO_REF:0000107

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:12526743

Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors.

PMID:15260970

Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs.

PMID:16289642

Identification of novel argonaute-associated proteins.

PMID:16756390

Translation repression in human cells by microRNA-induced gene silencing requires RCK/p54.

PMID:17891150

A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain.

PMID:17932509

Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells.

PMID:18482256

Protein microarray analysis identifies human cellular prion protein interactors.

PMID:18771919

Importance of translation and nonnucleolytic ago proteins for on-target RNA interference.

PMID:19167051

Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs.

PMID:19324964

The C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires GW182 to mediate silencing.

PMID:19383768

The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence bound transcripts independently of Argonaute proteins.

PMID:19716330

Mammalian miRNA RISC recruits CAF1 and PABP to affect PABP-dependent deadenylation.

PMID:19966796

ATP-dependent human RISC assembly pathways.

PMID:20014101

Mouse ZAR1-like (XM_359149) colocalizes with mRNA processing components and its dominant-negative mutant caused two-cell-stage embryonic arrest.

PMID:20616046

LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for microRNA-mediated gene silencing.

PMID:22484317

Human prion protein binds Argonaute and promotes accumulation of microRNA effector complexes.

PMID:22681889

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.

PMID:22795694

Slicing-independent RISC activation requires the argonaute PAZ domain.

PMID:22915799

HIV-1 replication and APOBEC3 antiviral activity are not regulated by P bodies.

PMID:23426184

Hypoxia-responsive miRNAs target argonaute 1 to promote angiogenesis.

PMID:23809764

Eukaryote-specific insertion elements control human ARGONAUTE slicer activity.

PMID:25036637

A quantitative chaperone interaction network reveals the architecture of cellular protein homeostasis pathways.

PMID:25336585

Cellular microRNAs up-regulate transcription via interaction with promoter TATA-box motifs.

PMID:28330616

Systematic Analysis of Human Protein Phosphatase Interactions and Dynamics.

PMID:28683311

Argonaute Utilization for miRNA Silencing Is Determined by Phosphorylation-Dependent Recruitment of LIM-Domain-Containing Proteins.

PMID:28781232

Multivalent Recruitment of Human Argonaute by GW182.

PMID:33961781

Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.

PMID:35271311

OpenCell: Endogenous tagging for the cartography of human cellular organization.

PMID:35709258

Spatial centrosome proteome of human neural cells uncovers disease-relevant heterogeneity.

PMID:40205054

Multimodal cell maps as a foundation for structural and functional genomics.

Reactome:R-HSA-1606561

MIR449 microRNAs bind 3'UTR of NOTCH1 mRNA

Reactome:R-HSA-1606682

MIR34 microRNAs bind 3'UTR of NOTCH1 mRNA

Reactome:R-HSA-1912362

MIR150 microRNA binds 3'UTR of NOTCH3 mRNA

Reactome:R-HSA-1912363

MIR200B/C microRNAs bind NOTCH1 mRNA

Reactome:R-HSA-1912364

MIR181C microRNA binds 3'UTR of NOTCH4 mRNA

Reactome:R-HSA-1912366

MIR206 microRNA binds 3'UTR of NOTCH3 mRNA

Reactome:R-HSA-1912367

MIR34 microRNAs bind 3'UTR of NOTCH2 mRNA

Reactome:R-HSA-1912368

MIR302A microRNA binds 3'UTR of NOTCH4 mRNA

Reactome:R-HSA-1912406

p53 positively regulates transcription of MIR34 microRNAs

Reactome:R-HSA-2318752

miR-26A microRNAs bind PTEN mRNA

Reactome:R-HSA-3209151

miR-24 binds p16INK4A and p14ARF mRNAs

Reactome:R-HSA-426489

RISC binds inexactly matching target RNAs

Reactome:R-HSA-426522

Nonendonucleolytic RISC binds exactly matching target RNAs

Reactome:R-HSA-4518575

miR-92b binds 3'UTR of NLK mRNA

Reactome:R-HSA-5578742

AGO1,2:small RNA complexes interact with chromatin

Reactome:R-HSA-5687103

FOXO3 regulates MIR34B,C expression

Reactome:R-HSA-8935766

miR-378 binds RUNX1 mRNA

Reactome:R-HSA-8937097

MIR27A gene transcription is stimulated by the complex containing RUNX1, PRMT1 and GATA1 and inhibited by the complex of RUNX1, SIN3A and PRMT6

Reactome:R-HSA-8937134

miR-27a binds RUNX1 mRNA

Reactome:R-HSA-8938440

miR-17 binds RUNX1 mRNA

Reactome:R-HSA-8938487

miR-20a binds RUNX1 mRNA

Reactome:R-HSA-8938507

miR-106a binds RUNX1 mRNA

Reactome:R-HSA-8944650

miR-214 microRNA binds PTEN mRNA

Reactome:R-HSA-8944684

miR-205 microRNA binds PTEN mRNA

Reactome:R-HSA-8944706

miR-21 nonendonucleolytic RISC binds PTEN mRNA

Reactome:R-HSA-9011958

miR-26A and B bind to the 3'UTR of the GREB1 mRNA

Reactome:R-HSA-9012203

miR-26A and B bind to the 3'UTR of the CHD11 mRNA

Reactome:R-HSA-9012208

miR-26A and B bind to the 3'UTR of the KPNA2 mRNA

Reactome:R-HSA-9038545

miR-613 binds to the 3'UTR of the NR1H3 mRNA

Reactome:R-HSA-9618392

miR-26 binds to the 3'UTR of the ARL4C mRNA

Reactome:R-HSA-9618486

miR-26 binds to the 3'UTR of the ABCA1 mRNA

Reactome:R-HSA-9624925

miR-33 binds to the 3'UTR of the ABCA1 mRNA

Reactome:R-HSA-9657791

miR-144 binds to the 3'UTR of the ABCA1 mRNA

Reactome:R-HSA-9858289

MITF-M-dependent miR-211 expression

Reactome:R-HSA-9858309

miR-211 RISC binds POU3F2 mRNA

Reactome:R-HSA-9924921

CD274 mRNA binds miR-34 RISC

Reactome:R-HSA-9925095

CD274 mRNA binds miR-340 RISC

Reactome:R-HSA-9925158

CD274 mRNA binds miR-152 RISC

Reactome:R-HSA-9925159

CD274 mRNA binds miR-140 RISC

Reactome:R-HSA-9925324

CD274 mRNA binds miR-200B/C RISC

file:human/AGO1/AGO1-uniprot.txt

UniProt text export for AGO1

UniProt identifies AGO1 and its protein family/domain context.

"Belongs to the argonaute family. Ago subfamily."

file:human/AGO1/AGO1-deep-research-falcon.md

Falcon deep research report for AGO1

Falcon research supports the reviewed core function of AGO1.

"Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay** through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways."

Suggested Experiments

Experiment: Endogenous tagging of AGO1 followed by small-RNA CLIP, target RNA profiling, and paralog-specific rescue in AGO-depleted human cells.

Hypothesis: AGO1 has paralog-specific guide, target, and compartment preferences within human RISC biology.

Type: genome editing/RNA-protein interaction profiling

Experiment: Biochemical reconstitution of AGO1-RISC with representative miRNA and siRNA guides to compare target repression, target cleavage, and TNRC6 recruitment.

Hypothesis: AGO1 activity is best explained by guide-loaded RISC function rather than generic RNA or protein binding.

Type: biochemical reconstitution

Deep Research

Falcon

(AGO1-deep-research-falcon.md)

this with annotations you find in gene/protein databases, but these can be outdated or inaccurate. Falcon Edison Scientific Literature 21 citations 2026-05-02T22:53:42.424236

The research report should be a detailed narrative explaining the function, biological processes, and localization of the gene product. Citations should be given for all claims.

You should prioritize authoritative reviews and primary scientific literature when conducting research. You can supplement
this with annotations you find in gene/protein databases, but these can be outdated or inaccurate.

We are specifically interested in the primary function of the gene - for enzymes, what reaction is catalyzed, and what is the substrate specificity? For transporters, what is the substrate? For structural proteins or adapters, what is the broader structural role? For signaling molecules, what is the role in the pathway.

We are interested in where in or outside the cell the gene product carries out its function.

We are also interested in the signaling or biochemical pathways in which the gene functions. We are less interested in broad pleiotropic effects, except where these elucidate the precise role.

Include evidence where possible. We are interested in both experimental evidence as well as inference from structure, evolution, or bioinformatic analysis. Precise studies should be prioritized over high-throughput, where available.

Comprehensive Research Report: Human AGO1 (EIF2C1) — Functional Annotation (UniProt Q9UL18)

1) Target verification (mandatory identity check)

The UniProt accession Q9UL18 corresponds to Homo sapiens Argonaute-1 (AGO1), also known as EIF2C1 and “Argonaute RISC catalytic component 1.” In humans there are four Argonaute paralogs (AGO1–AGO4) with a conserved Argonaute architecture (N, PAZ, MID, PIWI domains), and AGO1 is classified as a non-slicer Argonaute in contrast to AGO2, the principal human endonucleolytic (“slicer”) Argonaute. (narasimhan2025partnersinsilencing pages 4-5, carvalho2019studyofhuman pages 27-29)

2) Key concepts and definitions (current understanding)

2.1 Argonaute proteins and miRISC

Argonaute (AGO) proteins are the central effectors of RNA silencing pathways; they bind small RNAs (miRNAs/siRNAs) and use them as guides to recognize target RNAs, producing post-transcriptional gene silencing via translational repression and/or target RNA decay. (carvalho2019studyofhuman pages 27-29)

In the canonical mammalian pathway, a mature miRNA duplex is loaded into an AGO-containing complex (miRNA-induced silencing complex; miRISC). One strand becomes the guide; the other (passenger) is removed during RISC maturation. Target recognition occurs primarily through the miRNA seed region (nucleotides 2–8), often in 3′-UTRs. (narasimhan2025partnersinsilencing pages 2-4, narasimhan2025partnersinsilencing pages 1-2)

2.2 Domain architecture and what the domains do

Mammalian Argonautes (including AGO1) share four conserved domains:
- N domain: supports small-RNA duplex unwinding during RISC assembly. (carvalho2019studyofhuman pages 27-29)
- PAZ domain: anchors the 3′ end of the guide small RNA. (carvalho2019studyofhuman pages 27-29)
- MID domain: anchors the 5′ end of the guide small RNA. (carvalho2019studyofhuman pages 27-29)
- PIWI domain: adopts an RNase H-like fold; in humans, robust endonucleolytic cleavage is associated primarily with AGO2, not AGO1. (narasimhan2025partnersinsilencing pages 4-5, carvalho2019studyofhuman pages 27-29)

2.3 Enzymatic/catalytic function (substrate specificity)

For Argonautes, the “enzyme-like” activity is guide-directed endonucleolytic cleavage (slicing) of target RNAs when extensive guide–target complementarity exists.
- AGO1: non-slicer in human cells; it does not perform canonical guide-directed target cleavage, and it also cannot nick the passenger strand during loading like AGO2. Passenger-strand removal for AGO1 relies on mismatch-driven separation (notably aided by mismatches around guide positions 13–16), and the endonuclease responsible for passenger degradation in AGO1-containing complexes remains unclear in the cited review. (narasimhan2025partnersinsilencing pages 2-4, narasimhan2025partnersinsilencing pages 4-5, narasimhan2025partnersinsilencing pages 1-2)
- AGO2: principal slicer Argonaute in humans (used here as the key comparator). (narasimhan2025partnersinsilencing pages 4-5, carvalho2019studyofhuman pages 27-29)

3) Molecular function and mechanisms (pathways and partners)

3.1 RISC loading and maturation partners

Evidence from a mammalian Argonaute interactome review indicates that miRISC loading involves:
- DICER1 and dsRNA-binding partners TRBP and PACT (RISC loading complex),
- ATP-dependent chaperone machinery including HSC70 and HSP90, with cochaperones such as p23,
- and passenger-strand disposal factors such as C3PO (TSN/TRAX) in some contexts. (narasimhan2025partnersinsilencing pages 1-2)

3.2 Downstream repression: deadenylation, decapping, and decay

Once AGO1-miRISC binds targets (typically through seed interactions), repression is mediated largely through translational repression and mRNA destabilization pathways rather than slicing. (narasimhan2025partnersinsilencing pages 2-4, narasimhan2025partnersinsilencing pages 4-5)

Key effector modules described include:
- GW182/TNRC6 family proteins as scaffolds,
- recruitment of deadenylation machinery CCR4–NOT and PABP, with PAN2–PAN3 trimming poly(A) tails to ~50–110 nt (as summarized in the review),
- DDX6 linking CCR4–NOT to decapping enzymes DCP1/2,
- and subsequent exonucleolytic degradation by XRN1 or the exosome. (narasimhan2025partnersinsilencing pages 2-4, narasimhan2025partnersinsilencing pages 4-5)

4) Subcellular localization (where AGO1 acts) and regulation

4.1 Cytoplasmic gene silencing with partitioning into RNA granules

Canonical AGO1-miRISC activity is cytoplasmic (miRNA-guided target repression/decay). (carvalho2019studyofhuman pages 27-29, narasimhan2025partnersinsilencing pages 2-4)

AGO proteins (AGO1–AGO4) localize to stress granules, and AGO biology is intertwined with processing bodies (P-bodies), which are associated with mRNA decapping/decay pathways. (narasimhan2025partnersinsilencing pages 8-10, narasimhan2025partnersinsilencing pages 4-5)

4.2 Post-translational modifications (PTMs) that modulate localization and activity

A key review summarizes multiple PTMs affecting AGO proteins and provides evidence directly implicating AGO1:
- PARP-mediated poly(ADP-ribosyl)ation (PARylation) of AGO1–AGO4, with PARylated AGOs reported as mainly present in stress granules; PARylation can inhibit miRISC-mediated silencing (demonstrated for AGO2 in the reviewed work, but the modification applies to AGO1–4). (narasimhan2025partnersinsilencing pages 8-10, narasimhan2025partnersinsilencing media 7103b7a6)
- Hydroxylation-dependent P-body localization: mutation of AGO2 P700A (affecting a prolyl-4-hydroxylation site) reduced P-body localization of AGO1, AGO2, and AGO4 (but not AGO3) without affecting stress-granule localization in the cited experiments, suggesting cross-AGO coupling of localization control. (narasimhan2025partnersinsilencing pages 8-10)
- Enzymes reported to modify AGO1 in the same review include RANBP2/UBC9 (SUMOylation; AGO1/2), CSNK1A1 (phosphorylation; AGO1–3), and STUB1 (K48 polyubiquitination; AGO1–4). (narasimhan2025partnersinsilencing pages 8-10, narasimhan2025partnersinsilencing media 7103b7a6)

(Visual support) Table 2 summarizing these PTMs and enzymes is available as extracted figure/table evidence. (narasimhan2025partnersinsilencing media 7103b7a6, narasimhan2025partnersinsilencing media 17086596)

4.3 RNP remodeling and polysome/P-body partitioning

STAU2 (an RNA-binding protein) is reported to modulate AGO1 abundance and shift AGO1/2 RNP assemblies from P-bodies toward polysomes, with consequences for translation of miRNA targets. (narasimhan2025partnersinsilencing pages 11-13)

4.4 ER-associated condensates

AGO family proteins contain an N-terminal lipid-binding motif that binds PI(4,5)P2 and promotes condensation into phase-separated granules on the endoplasmic reticulum (ER), implying ER-associated spatial organization of AGO-containing RNPs (including AGO1 as part of “AGOs” in the cited review). (narasimhan2025partnersinsilencing pages 11-13)

4.5 AGO1 isoform: translational readthrough product (AGO1x)

A translational readthrough isoform AGO1x is reported as particularly expressed in brain (and “to a lesser extend” in heart, kidney, and muscle). Let-7a promotes readthrough and increases AGO1x expression. AGO1x can load miRNAs and bind DICER and targets, but it cannot interact with GW182/TNRC6A, so AGO1x-RISC does not repress targets. Overexpression in HeLa cells increased global translation and decreased the number of P-bodies. (narasimhan2025partnersinsilencing pages 10-11)

5) Recent developments and latest research (prioritizing 2023–2024)

5.1 Human genetics: AGO1 as a neurodevelopmental disorder gene

A 2024 PNAS study modeling disease variants reports that exome studies identified 18 de novo coding AGO1 variants in affected children (and 12 in AGO2), linking AGO1 to neurodevelopmental disorder (NDD)/intellectual disability and often autism-spectrum features. (2024-02; URL: https://doi.org/10.1073/pnas.2308255121) (duan2024modelingneurodevelopmentaldisorderassociated pages 1-2)

A 2023 bioRxiv preprint from the same group similarly reports 18 de novo AGO1 variants and provides parallel mechanistic interpretation. (2023-04; URL: https://doi.org/10.1101/2023.04.06.535748) (duan2023modelingneurodevelopmentaldisorderassociated pages 1-4)

5.2 Functional interpretation via cross-species modeling

The 2024 PNAS study engineered four human AGO1 NDD mutations into the C. elegans AGO1 ortholog (alg-1) and found that these alleles disrupt miRNA-centered regulation with allele-specific changes in mature miRNA profiles and downstream gene expression, including altered translational efficiency and/or mRNA abundance. The authors conclude these alleles act antimorphically (dominant-negative behavior), producing defects stronger than null alleles—consistent with sequestration of functional miRISC components into nonfunctional complexes. (duan2024modelingneurodevelopmentaldisorderassociated pages 1-2)

6) Current applications and real-world implementations

6.1 RNAi/miRNA technologies (mechanism-based implementation)

Although most therapeutic development focuses on delivering small RNAs (siRNAs, miRNA mimics, antimiRs) rather than targeting AGO1 directly, these technologies depend on:
- efficient RISC loading,
- Argonaute partner factors (e.g., DICER/TRBP/PACT; chaperones),
- and functional recruitment of effector modules (e.g., TNRC6/CCR4–NOT/DDX6/decapping) that execute repression/decay. (narasimhan2025partnersinsilencing pages 1-2, narasimhan2025partnersinsilencing pages 2-4, narasimhan2025partnersinsilencing pages 4-5)

From a rare-disease review perspective, abnormal miRNA processing/regulation is increasingly implicated in Mendelian conditions, and defects in RISC components (including AGO1/2) are linked to neurodevelopmental pathology, motivating diagnostic interpretation of pathway variants. (2024-09; URL: https://doi.org/10.3390/genes15101243) (cuk2024noveldenovo pages 7-8)

6.2 Practical research implementations (assays and omics)

Mechanistic studies of Argonaute function commonly use immunoprecipitation-based approaches (e.g., AGO-CLIP variants) to identify miRNA:target interactions and RNP partners; while the retrieved nuclear-Argonaute primary study was AGO2-focused, the broader principle is that Argonaute-bound small RNAs and their ligated targets can be mapped transcriptome-wide to infer functional networks and potential therapeutic nodes. (ding2025thelifeof pages 25-27)

7) Expert opinions and authoritative analyses (interpretation)

7.1 Mechanistic consensus: AGO1 is a non-slicer effector specialized for repression/decay

A mechanistic consensus emerging from reviewed work is that mammalian AGO paralogs share core architecture and many partners but differ in catalytic ability; AGO1/3/4 are non-slicer, and repression is typically mediated through recruitment of GW182/TNRC6 and downstream deadenylation/decapping pathways rather than direct cleavage. (narasimhan2025partnersinsilencing pages 2-4, narasimhan2025partnersinsilencing pages 4-5)

7.2 Regulation is multi-layered and context dependent

The Argonaute interactome review emphasizes that AGO stability, localization, and silencing efficiency depend strongly on RNP composition and PTMs, including stress-linked PARylation and modifications that alter P-body/stress granule partitioning. (narasimhan2025partnersinsilencing pages 8-10, narasimhan2025partnersinsilencing pages 10-11)

8) Relevant statistics and data points (from recent studies and databases)

18 de novo AGO1 coding variants reported from exome studies of affected children with neurodevelopmental disorder phenotypes (as summarized in 2024 PNAS; also repeated in 2023 preprint). (duan2024modelingneurodevelopmentaldisorderassociated pages 1-2, duan2023modelingneurodevelopmentaldisorderassociated pages 1-4)
Poly(A) trimming length: PAN2–PAN3 shortens poly(A) tails to approximately 50–110 nt in the reviewed miRISC repression model. (narasimhan2025partnersinsilencing pages 2-4)
Open Targets evidence scores (database integration): AGO1 association score reported as ~0.803 for “neurodevelopmental disorder with language delay and behavioral abnormalities, with or without seizures,” and ~0.488 for “cancer” (Open Targets). ()

9) Limitations of the retrieved corpus (important for correctness)

Despite targeted searches, the tool-retrieved set contained limited 2023–2024 authoritative human-AGO1–specific mechanistic reviews; mechanistic detail was therefore primarily supported by an accessible recent Argonaute interactome review (2025) plus human-AGO1 disease-mechanism primary research (2024 PNAS) and a 2023 preprint. (narasimhan2025partnersinsilencing pages 2-4, duan2024modelingneurodevelopmentaldisorderassociated pages 1-2, duan2023modelingneurodevelopmentaldisorderassociated pages 1-4)
Nuclear Argonaute functions in mammals are an active area, but AGO1-specific nuclear mechanistic evidence was not directly retrieved in the accessible texts; conclusions here are therefore restricted to supported statements about cytoplasmic silencing and RNA-granule partitioning for AGO1. (narasimhan2025partnersinsilencing pages 8-10, narasimhan2025partnersinsilencing pages 11-13)

Summary (functional annotation)

Human AGO1 (EIF2C1; UniProt Q9UL18) is a non-slicer Argonaute that functions as a core miRISC effector mediating miRNA-guided repression and mRNA decay through recruitment of TNRC6/GW182 and downstream deadenylation/decapping pathways. AGO1 operates predominantly in the cytoplasm and dynamically partitions into P-bodies, stress granules, and likely ER-associated condensates, with localization and activity tuned by PTMs (e.g., PARylation in stress granules; hydroxylation-linked control of P-body localization) and RNP remodeling factors (e.g., STAU2). Recent human genetics and functional modeling (2023–2024) strongly implicate de novo AGO1 variants in neurodevelopmental disorders, supporting the view that partial disruption of miRNA-centered regulation can have broad neurodevelopmental impact. (narasimhan2025partnersinsilencing pages 2-4, narasimhan2025partnersinsilencing pages 4-5, duan2024modelingneurodevelopmentaldisorderassociated pages 1-2, narasimhan2025partnersinsilencing pages 8-10, narasimhan2025partnersinsilencing pages 11-13)

Aspect	Human AGO1 summary	Key details	Best supporting citations	Year / URL
Identity	AGO1 = human argonaute RISC component 1 (EIF2C1), UniProt Q9UL18	Member of mammalian AGO1-4 subfamily; core effector of miRNA-loaded RISC; conserved N, PAZ, MID, PIWI domains	(narasimhan2025partnersinsilencing pages 4-5, carvalho2019studyofhuman pages 27-29)	2019; 2025; https://doi.org/10.3390/ncrna11040062
Core molecular function in miRISC	Small-RNA loading, guide stabilization, target recognition, post-transcriptional repression	Pre-miRNA processing/loading involves DICER1 with TRBP/PACT; AGO loading requires HSC70/HSP90/p23; AGO1-bound miRNAs recognize targets mainly through seed nt 2-8, usually in 3′ UTRs; repression proceeds largely via translational repression plus deadenylation/decapping-linked decay rather than slicing	(narasimhan2025partnersinsilencing pages 2-4, narasimhan2025partnersinsilencing pages 1-2)	2025; https://doi.org/10.3390/ncrna11040062
Catalytic status vs AGO2	AGO1 is a non-slicer AGO; AGO2 is the principal human slicer	AGO1/3/4 cannot nick passenger strand like AGO2; AGO1 lacks the catalytic tetrad associated with AGO2 slicing; passenger-strand removal instead exploits duplex mismatches, especially guide positions 13-16	(narasimhan2025partnersinsilencing pages 2-4, narasimhan2025partnersinsilencing pages 4-5, carvalho2019studyofhuman pages 27-29)	2019; 2025; https://doi.org/10.3390/ncrna11040062
Key interactors: loading/assembly	DICER1, TRBP, PACT, HSC70, HSP90, p23, C3PO	These factors support pre-miRNA processing, RISC loading, AGO opening/folding, and passenger-strand disposal; complete passenger degradation can be executed by C3PO (TSN/TRAX)	(narasimhan2025partnersinsilencing pages 1-2)	2025; https://doi.org/10.3390/ncrna11040062
Key interactors: repression/decay	TNRC6/GW182, CCR4-NOT, PABP, PAN2-PAN3, PARN, DDX6, DCP1/2, XRN1, exosome	GW182/TNRC6 scaffolds recruit CCR4-NOT/PABP deadenylation machinery; PAN2-PAN3 shortens poly(A) tails to ~50-110 nt; DDX6 links CCR4-NOT to decapping; DCP1/2 support decapping; XRN1/exosome degrade decapped transcripts	(narasimhan2025partnersinsilencing pages 2-4, narasimhan2025partnersinsilencing pages 4-5)	2025; https://doi.org/10.3390/ncrna11040062
Subcellular localization / context	Cytoplasm, P-bodies, stress granules, ER-associated condensates; some evidence for nuclear roles across mammalian Argonautes	Canonical repression is cytoplasmic; AGO1 localizes to P-bodies; AGO1-4 localize to stress granules; AGO-family N-terminal lipid-binding motif can drive PI(4,5)P2-dependent ER-associated condensates; miRISC composition influences localization	(narasimhan2025partnersinsilencing pages 8-10, narasimhan2025partnersinsilencing pages 11-13)	2025; https://doi.org/10.3390/ncrna11040062
Post-translational modifications	SUMOylation, phosphorylation, ubiquitination, PARylation	RANBP2 promotes AGO1/2 sumoylation; CSNK1A1 phosphorylates AGO1-3 EI region; STUB1 adds K48 polyubiquitin to AGO1-4; PARP poly(ADP-ribosyl)ates AGO1-4 mainly in stress granules and can inhibit miRISC repression under stress	(narasimhan2025partnersinsilencing pages 8-10, narasimhan2025partnersinsilencing media 7103b7a6)	2025; https://doi.org/10.3390/ncrna11040062
Specialized isoform	AGO1x translational readthrough isoform	Let-7a promotes readthrough; AGO1x binds miRNA, DICER, and target mRNA but does not bind GW182/TNRC6A, so AGO1x-RISC fails to repress targets; overexpression increases global translation and reduces processing bodies	(narasimhan2025partnersinsilencing pages 10-11)	2025; https://doi.org/10.3390/ncrna11040062
Disease relevance	Strongest current human genetic link is neurodevelopmental disorder	Exome studies identified 18 de novo AGO1 coding variants in affected children; modeling of AGO1 NDD alleles in C. elegans supports disruption of miRNA biogenesis, miRISC assembly, and target repression, with antimorphic effects stronger than nulls	(duan2024modelingneurodevelopmentaldisorderassociated pages 1-2, duan2023modelingneurodevelopmentaldisorderassociated pages 1-4)	2023 preprint; 2024 peer-reviewed; https://doi.org/10.1101/2023.04.06.535748 ; https://doi.org/10.1073/pnas.2308255121
Broader translational relevance	AGO1 biology underpins miRNA/RNAi therapeutics and diagnostics, though AGO1 itself is not a direct approved drug target	RISC loading, Argonaute localization, and Argonaute-partner interactions are foundational for small-RNA drug action, biomarker interpretation, and design of miRNA/siRNA strategies; disease-linked AGO1 variants highlight pathway vulnerability in humans	(ding2025thelifeof pages 25-27, cuk2024noveldenovo pages 7-8)	2024; 2025; https://doi.org/10.3390/genes15101243 ; https://doi.org/10.3390/biom15101393

Table: This table condenses the main functional, mechanistic, localization, modification, and disease-association evidence for human AGO1/EIF2C1. It is useful as a quick reference for building the full research report while keeping each claim tied to available context IDs and source URLs.

References

(narasimhan2025partnersinsilencing pages 4-5): Srinaath Narasimhan and Stefan J. Erkeland. Partners in silencing: decoding the mammalian argonaute interactome. Non-Coding RNA, 11:62, Aug 2025. URL: https://doi.org/10.3390/ncrna11040062, doi:10.3390/ncrna11040062. This article has 4 citations.
(carvalho2019studyofhuman pages 27-29): MNCRR Carvalho. Study of human argonaute 1 cap-independent translation. Unknown journal, 2019.
(narasimhan2025partnersinsilencing pages 2-4): Srinaath Narasimhan and Stefan J. Erkeland. Partners in silencing: decoding the mammalian argonaute interactome. Non-Coding RNA, 11:62, Aug 2025. URL: https://doi.org/10.3390/ncrna11040062, doi:10.3390/ncrna11040062. This article has 4 citations.
(narasimhan2025partnersinsilencing pages 1-2): Srinaath Narasimhan and Stefan J. Erkeland. Partners in silencing: decoding the mammalian argonaute interactome. Non-Coding RNA, 11:62, Aug 2025. URL: https://doi.org/10.3390/ncrna11040062, doi:10.3390/ncrna11040062. This article has 4 citations.
(narasimhan2025partnersinsilencing pages 8-10): Srinaath Narasimhan and Stefan J. Erkeland. Partners in silencing: decoding the mammalian argonaute interactome. Non-Coding RNA, 11:62, Aug 2025. URL: https://doi.org/10.3390/ncrna11040062, doi:10.3390/ncrna11040062. This article has 4 citations.
(narasimhan2025partnersinsilencing media 7103b7a6): Srinaath Narasimhan and Stefan J. Erkeland. Partners in silencing: decoding the mammalian argonaute interactome. Non-Coding RNA, 11:62, Aug 2025. URL: https://doi.org/10.3390/ncrna11040062, doi:10.3390/ncrna11040062. This article has 4 citations.
(narasimhan2025partnersinsilencing media 17086596): Srinaath Narasimhan and Stefan J. Erkeland. Partners in silencing: decoding the mammalian argonaute interactome. Non-Coding RNA, 11:62, Aug 2025. URL: https://doi.org/10.3390/ncrna11040062, doi:10.3390/ncrna11040062. This article has 4 citations.
(narasimhan2025partnersinsilencing pages 11-13): Srinaath Narasimhan and Stefan J. Erkeland. Partners in silencing: decoding the mammalian argonaute interactome. Non-Coding RNA, 11:62, Aug 2025. URL: https://doi.org/10.3390/ncrna11040062, doi:10.3390/ncrna11040062. This article has 4 citations.
(narasimhan2025partnersinsilencing pages 10-11): Srinaath Narasimhan and Stefan J. Erkeland. Partners in silencing: decoding the mammalian argonaute interactome. Non-Coding RNA, 11:62, Aug 2025. URL: https://doi.org/10.3390/ncrna11040062, doi:10.3390/ncrna11040062. This article has 4 citations.
(duan2024modelingneurodevelopmentaldisorderassociated pages 1-2): Ye Duan, Li Li, Ganesh Prabhakar Panzade, Amélie Piton, Anna Zinovyeva, and Victor Ambros. Modeling neurodevelopmental disorder-associated human ago1 mutations in caenorhabditis elegans argonaute alg-1. Proceedings of the National Academy of Sciences of the United States of America, Feb 2024. URL: https://doi.org/10.1073/pnas.2308255121, doi:10.1073/pnas.2308255121. This article has 20 citations and is from a highest quality peer-reviewed journal.
(duan2023modelingneurodevelopmentaldisorderassociated pages 1-4): Ye Duan, Li Li, Ganesh Prabhakar Panzade, Amélie Piton, Anna Zinovyeva, and Victor Ambros. Modeling neurodevelopmental disorder-associated hago1 mutations in c. elegans argonaute alg-1. bioRxiv, Apr 2023. URL: https://doi.org/10.1101/2023.04.06.535748, doi:10.1101/2023.04.06.535748. This article has 1 citations.
(cuk2024noveldenovo pages 7-8): Mario Ćuk, Luka Lovrenčić, Busra Unal, McKenzie Walker, Connor P. Hayes, Goran Krakar, Robert Beluzić, Ivona Sansović, Goran Pavliša, and Arezou A. Ghazani. Novel de novo nonsense variants in ago3 and khsrp: insights into global developmental delay and autism spectrum disorders through whole genome analysis. The American Journal of Case Reports, 25:e943641-1-e943641-8, Jun 2024. URL: https://doi.org/10.12659/ajcr.943641, doi:10.12659/ajcr.943641. This article has 0 citations.
(ding2025thelifeof pages 25-27): Shuang Ding and Pingping Wang. The life of micrornas: biogenesis, function and decay in cancer. Biomolecules, Sep 2025. URL: https://doi.org/10.3390/biom15101393, doi:10.3390/biom15101393. This article has 9 citations.

Citations

carvalho2019studyofhuman pages 27-29
narasimhan2025partnersinsilencing pages 1-2
narasimhan2025partnersinsilencing pages 8-10
narasimhan2025partnersinsilencing pages 11-13
narasimhan2025partnersinsilencing pages 10-11
duan2024modelingneurodevelopmentaldisorderassociated pages 1-2
duan2023modelingneurodevelopmentaldisorderassociated pages 1-4
cuk2024noveldenovo pages 7-8
ding2025thelifeof pages 25-27
narasimhan2025partnersinsilencing pages 2-4
narasimhan2025partnersinsilencing pages 4-5
https://doi.org/10.1073/pnas.2308255121
https://doi.org/10.1101/2023.04.06.535748
https://doi.org/10.3390/genes15101243
https://doi.org/10.3390/ncrna11040062
https://doi.org/10.3390/biom15101393
https://doi.org/10.3390/ncrna11040062,
https://doi.org/10.1073/pnas.2308255121,
https://doi.org/10.1101/2023.04.06.535748,
https://doi.org/10.12659/ajcr.943641,
https://doi.org/10.3390/biom15101393,

📄 View Raw YAML

id: Q9UL18
gene_symbol: AGO1
product_type: PROTEIN
status: COMPLETE
taxon:
  id: NCBITaxon:9606
  label: Homo sapiens
description: 'AGO1 encodes human Argonaute-1, a non-slicer Argonaute paralog that binds miRNAs in miRISC
  and mediates post-transcriptional repression through TNRC6/GW182-linked deadenylation, decapping, and
  mRNA decay pathways. Its core role is small-RNA-guided repression in cytoplasmic RISC/P-body-associated
  ribonucleoprotein complexes, with additional non-core nuclear and disease-context observations.'
existing_annotations:
- term:
    id: GO:0005634
    label: nucleus
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: nucleus is a plausible or reported location/context but is not the core functional site
      annotation for AGO1.
    action: KEEP_AS_NON_CORE
    reason: The core location is cytoplasmic RISC/RNP granules; nuclear, membrane, synaptic,
      exosomal, or other compartment annotations should remain non-core unless tied to a specific
      curated mechanism.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005737
    label: cytoplasm
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: cytoplasm is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0035194
    label: regulatory ncRNA-mediated post-transcriptional gene silencing
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: regulatory ncRNA-mediated post-transcriptional gene silencing is consistent with AGO1
      as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0016442
    label: RISC complex
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: RISC complex is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0036464
    label: cytoplasmic ribonucleoprotein granule
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: cytoplasmic ribonucleoprotein granule is consistent with AGO1 as a small-RNA-loaded
      Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0031054
    label: pre-miRNA processing
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: pre-miRNA processing overstates the direct role of AGO1 in canonical miRNA biogenesis.
    action: MARK_AS_OVER_ANNOTATED
    reason: The better-supported role is as a loaded Argonaute effector after small-RNA processing,
      not as the Drosha/Dicer processing enzyme for pre-miRNAs.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0035198
    label: miRNA binding
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: miRNA binding is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0003727
    label: single-stranded RNA binding
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: single-stranded RNA binding is too generic for AGO1.
    action: MARK_AS_OVER_ANNOTATED
    reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC
      rather than a broad nucleic-acid or RNA-binding label.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0000932
    label: P-body
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  review:
    summary: P-body is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0003676
    label: nucleic acid binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000002
  review:
    summary: nucleic acid binding is too generic for AGO1.
    action: MARK_AS_OVER_ANNOTATED
    reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC
      rather than a broad nucleic-acid or RNA-binding label.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0003723
    label: RNA binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  review:
    summary: RNA binding is too generic for AGO1.
    action: MARK_AS_OVER_ANNOTATED
    reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC
      rather than a broad nucleic-acid or RNA-binding label.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0003725
    label: double-stranded RNA binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: double-stranded RNA binding is too generic for AGO1.
    action: MARK_AS_OVER_ANNOTATED
    reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC
      rather than a broad nucleic-acid or RNA-binding label.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0003727
    label: single-stranded RNA binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: single-stranded RNA binding is too generic for AGO1.
    action: MARK_AS_OVER_ANNOTATED
    reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC
      rather than a broad nucleic-acid or RNA-binding label.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0031054
    label: pre-miRNA processing
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: pre-miRNA processing overstates the direct role of AGO1 in canonical miRNA biogenesis.
    action: MARK_AS_OVER_ANNOTATED
    reason: The better-supported role is as a loaded Argonaute effector after small-RNA processing,
      not as the Drosha/Dicer processing enzyme for pre-miRNAs.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0035278
    label: miRNA-mediated gene silencing by inhibition of translation
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: miRNA-mediated gene silencing by inhibition of translation is consistent with AGO1 as a
      small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0036464
    label: cytoplasmic ribonucleoprotein granule
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: cytoplasmic ribonucleoprotein granule is consistent with AGO1 as a small-RNA-loaded
      Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0070578
    label: RISC-loading complex
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: RISC-loading complex is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC
      effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0070922
    label: RISC complex assembly
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: RISC complex assembly is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC
      effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:12526743
  review:
    summary: Generic protein binding does not capture the specific Argonaute/RISC function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The cited interactions are best interpreted as RISC/cofactor context for
      small-RNA-guided silencing, not as a standalone core function.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:16756390
  review:
    summary: Generic protein binding does not capture the specific Argonaute/RISC function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The cited interactions are best interpreted as RISC/cofactor context for
      small-RNA-guided silencing, not as a standalone core function.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:17891150
  review:
    summary: Generic protein binding does not capture the specific Argonaute/RISC function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The cited interactions are best interpreted as RISC/cofactor context for
      small-RNA-guided silencing, not as a standalone core function.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:17932509
  review:
    summary: Generic protein binding does not capture the specific Argonaute/RISC function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The cited interactions are best interpreted as RISC/cofactor context for
      small-RNA-guided silencing, not as a standalone core function.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:18482256
  review:
    summary: Generic protein binding does not capture the specific Argonaute/RISC function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The cited interactions are best interpreted as RISC/cofactor context for
      small-RNA-guided silencing, not as a standalone core function.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:19167051
  review:
    summary: Generic protein binding does not capture the specific Argonaute/RISC function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The cited interactions are best interpreted as RISC/cofactor context for
      small-RNA-guided silencing, not as a standalone core function.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:19324964
  review:
    summary: Generic protein binding does not capture the specific Argonaute/RISC function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The cited interactions are best interpreted as RISC/cofactor context for
      small-RNA-guided silencing, not as a standalone core function.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:19383768
  review:
    summary: Generic protein binding does not capture the specific Argonaute/RISC function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The cited interactions are best interpreted as RISC/cofactor context for
      small-RNA-guided silencing, not as a standalone core function.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:19716330
  review:
    summary: Generic protein binding does not capture the specific Argonaute/RISC function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The cited interactions are best interpreted as RISC/cofactor context for
      small-RNA-guided silencing, not as a standalone core function.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:22484317
  review:
    summary: Generic protein binding does not capture the specific Argonaute/RISC function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The cited interactions are best interpreted as RISC/cofactor context for
      small-RNA-guided silencing, not as a standalone core function.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:25036637
  review:
    summary: Generic protein binding does not capture the specific Argonaute/RISC function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The cited interactions are best interpreted as RISC/cofactor context for
      small-RNA-guided silencing, not as a standalone core function.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:28330616
  review:
    summary: Generic protein binding does not capture the specific Argonaute/RISC function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The cited interactions are best interpreted as RISC/cofactor context for
      small-RNA-guided silencing, not as a standalone core function.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:28683311
  review:
    summary: Generic protein binding does not capture the specific Argonaute/RISC function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The cited interactions are best interpreted as RISC/cofactor context for
      small-RNA-guided silencing, not as a standalone core function.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:33961781
  review:
    summary: Generic protein binding does not capture the specific Argonaute/RISC function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The cited interactions are best interpreted as RISC/cofactor context for
      small-RNA-guided silencing, not as a standalone core function.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:35271311
  review:
    summary: Generic protein binding does not capture the specific Argonaute/RISC function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The cited interactions are best interpreted as RISC/cofactor context for
      small-RNA-guided silencing, not as a standalone core function.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:35709258
  review:
    summary: Generic protein binding does not capture the specific Argonaute/RISC function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The cited interactions are best interpreted as RISC/cofactor context for
      small-RNA-guided silencing, not as a standalone core function.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:40205054
  review:
    summary: Generic protein binding does not capture the specific Argonaute/RISC function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The cited interactions are best interpreted as RISC/cofactor context for
      small-RNA-guided silencing, not as a standalone core function.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0000976
    label: transcription cis-regulatory region binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: transcription cis-regulatory region binding reflects a reported nuclear or regulatory
      context rather than the main conserved AGO1 function.
    action: KEEP_AS_NON_CORE
    reason: Argonaute paralogs have reported nuclear/regulatory interactions, but the dominant
      conserved function remains small-RNA-guided post-transcriptional repression.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0000978
    label: RNA polymerase II cis-regulatory region sequence-specific DNA binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: RNA polymerase II cis-regulatory region sequence-specific DNA binding reflects a
      reported nuclear or regulatory context rather than the main conserved AGO1 function.
    action: KEEP_AS_NON_CORE
    reason: Argonaute paralogs have reported nuclear/regulatory interactions, but the dominant
      conserved function remains small-RNA-guided post-transcriptional repression.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005634
    label: nucleus
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: nucleus is a plausible or reported location/context but is not the core functional site
      annotation for AGO1.
    action: KEEP_AS_NON_CORE
    reason: The core location is cytoplasmic RISC/RNP granules; nuclear, membrane, synaptic,
      exosomal, or other compartment annotations should remain non-core unless tied to a specific
      curated mechanism.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0016442
    label: RISC complex
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  review:
    summary: RISC complex is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0035198
    label: miRNA binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  review:
    summary: miRNA binding is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:1901224
    label: positive regulation of non-canonical NF-kappaB signal transduction
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: positive regulation of non-canonical NF-kappaB signal transduction reflects a reported
      nuclear or regulatory context rather than the main conserved AGO1 function.
    action: KEEP_AS_NON_CORE
    reason: Argonaute paralogs have reported nuclear/regulatory interactions, but the dominant
      conserved function remains small-RNA-guided post-transcriptional repression.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0035196
    label: miRNA processing
  evidence_type: NAS
  original_reference_id: PMID:28781232
  review:
    summary: miRNA processing overstates the direct role of AGO1 in canonical miRNA biogenesis.
    action: MARK_AS_OVER_ANNOTATED
    reason: The better-supported role is as a loaded Argonaute effector after small-RNA processing,
      not as the Drosha/Dicer processing enzyme for pre-miRNAs.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0036464
    label: cytoplasmic ribonucleoprotein granule
  evidence_type: IDA
  original_reference_id: GO_REF:0000052
  review:
    summary: cytoplasmic ribonucleoprotein granule is consistent with AGO1 as a small-RNA-loaded
      Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005737
    label: cytoplasm
  evidence_type: IDA
  original_reference_id: PMID:17932509
  review:
    summary: cytoplasm is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0016525
    label: negative regulation of angiogenesis
  evidence_type: IMP
  original_reference_id: PMID:23426184
  review:
    summary: negative regulation of angiogenesis is downstream or context-specific for AGO1.
    action: KEEP_AS_NON_CORE
    reason: This process may be supported in a particular experiment or pathway model, but it is
      peripheral to the core Argonaute/RISC molecular role.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0045944
    label: positive regulation of transcription by RNA polymerase II
  evidence_type: IMP
  original_reference_id: PMID:25336585
  review:
    summary: positive regulation of transcription by RNA polymerase II reflects a reported nuclear
      or regulatory context rather than the main conserved AGO1 function.
    action: KEEP_AS_NON_CORE
    reason: Argonaute paralogs have reported nuclear/regulatory interactions, but the dominant
      conserved function remains small-RNA-guided post-transcriptional repression.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0001046
    label: core promoter sequence-specific DNA binding
  evidence_type: IMP
  original_reference_id: PMID:25336585
  review:
    summary: core promoter sequence-specific DNA binding reflects a reported nuclear or regulatory
      context rather than the main conserved AGO1 function.
    action: KEEP_AS_NON_CORE
    reason: Argonaute paralogs have reported nuclear/regulatory interactions, but the dominant
      conserved function remains small-RNA-guided post-transcriptional repression.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005634
    label: nucleus
  evidence_type: IC
  original_reference_id: PMID:25336585
  review:
    summary: nucleus is a plausible or reported location/context but is not the core functional site
      annotation for AGO1.
    action: KEEP_AS_NON_CORE
    reason: The core location is cytoplasmic RISC/RNP granules; nuclear, membrane, synaptic,
      exosomal, or other compartment annotations should remain non-core unless tied to a specific
      curated mechanism.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0000993
    label: RNA polymerase II complex binding
  evidence_type: IDA
  original_reference_id: PMID:25336585
  review:
    summary: RNA polymerase II complex binding reflects a reported nuclear or regulatory context
      rather than the main conserved AGO1 function.
    action: KEEP_AS_NON_CORE
    reason: Argonaute paralogs have reported nuclear/regulatory interactions, but the dominant
      conserved function remains small-RNA-guided post-transcriptional repression.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:25336585
  review:
    summary: Generic protein binding does not capture the specific Argonaute/RISC function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The cited interactions are best interpreted as RISC/cofactor context for
      small-RNA-guided silencing, not as a standalone core function.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0035196
    label: miRNA processing
  evidence_type: IMP
  original_reference_id: PMID:22795694
  review:
    summary: miRNA processing overstates the direct role of AGO1 in canonical miRNA biogenesis.
    action: MARK_AS_OVER_ANNOTATED
    reason: The better-supported role is as a loaded Argonaute effector after small-RNA processing,
      not as the Drosha/Dicer processing enzyme for pre-miRNAs.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0031054
    label: pre-miRNA processing
  evidence_type: IDA
  original_reference_id: PMID:19966796
  review:
    summary: pre-miRNA processing overstates the direct role of AGO1 in canonical miRNA biogenesis.
    action: MARK_AS_OVER_ANNOTATED
    reason: The better-supported role is as a loaded Argonaute effector after small-RNA processing,
      not as the Drosha/Dicer processing enzyme for pre-miRNAs.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0003725
    label: double-stranded RNA binding
  evidence_type: IDA
  original_reference_id: PMID:19966796
  review:
    summary: double-stranded RNA binding is too generic for AGO1.
    action: MARK_AS_OVER_ANNOTATED
    reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC
      rather than a broad nucleic-acid or RNA-binding label.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0003727
    label: single-stranded RNA binding
  evidence_type: IDA
  original_reference_id: PMID:19966796
  review:
    summary: single-stranded RNA binding is too generic for AGO1.
    action: MARK_AS_OVER_ANNOTATED
    reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC
      rather than a broad nucleic-acid or RNA-binding label.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0016442
    label: RISC complex
  evidence_type: IDA
  original_reference_id: PMID:19966796
  review:
    summary: RISC complex is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0070578
    label: RISC-loading complex
  evidence_type: IDA
  original_reference_id: PMID:19966796
  review:
    summary: RISC-loading complex is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC
      effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0070922
    label: RISC complex assembly
  evidence_type: IDA
  original_reference_id: PMID:19966796
  review:
    summary: RISC complex assembly is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC
      effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0090625
    label: siRNA-mediated gene silencing by mRNA destabilization
  evidence_type: IDA
  original_reference_id: PMID:15260970
  negated: true
  review:
    summary: This NOT annotation is appropriate; AGO1 is not the Argonaute paralog that mediates
      siRNA-guided target mRNA cleavage/destabilization in this study.
    action: ACCEPT
    reason: PMID:15260970 supports AGO1 association with small RNAs/RISC but shows that
      endonucleolytic target cleavage activity is associated with AGO2, not AGO1. UniProt also
      states that AGO1 lacks endonuclease activity and does not appear to cleave target mRNAs.
    supported_by:
    - reference_id: PMID:15260970
      supporting_text: Purification of the FLAG/HA-epitope-tagged Ago containing complexes from
        different human cell lines revealed that endonuclease activity is exclusively associated
        with Ago2.
    - reference_id: file:human/AGO1/AGO1-uniprot.txt
      supporting_text: Lacks endonuclease activity and does not appear to cleave target mRNAs.
- term:
    id: GO:0005737
    label: cytoplasm
  evidence_type: IDA
  original_reference_id: PMID:15260970
  review:
    summary: cytoplasm is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0016442
    label: RISC complex
  evidence_type: IDA
  original_reference_id: PMID:15260970
  review:
    summary: RISC complex is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0035198
    label: miRNA binding
  evidence_type: IDA
  original_reference_id: PMID:15260970
  review:
    summary: miRNA binding is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0035279
    label: miRNA-mediated gene silencing by mRNA destabilization
  evidence_type: IDA
  original_reference_id: PMID:15260970
  negated: true
  review:
    summary: This NOT annotation is appropriate; PMID:15260970 does not support assigning AGO1 to
      AGO2-like miRNA-guided target mRNA cleavage/destabilization.
    action: ACCEPT
    reason: AGO1 binds miRNAs and participates in miRISC, but the cited work and UniProt distinguish
      this non-slicer role from AGO2-dependent target RNA cleavage. The NOT annotation should be
      retained rather than converted into a positive AGO1 mRNA-destabilization function.
    supported_by:
    - reference_id: PMID:15260970
      supporting_text: The specific role of Ago2 in guiding target RNA cleavage was confirmed
        independently by siRNA-based depletion of individual Ago members in combination with a
        sensitive positive-readout reporter assay.
    - reference_id: file:human/AGO1/AGO1-uniprot.txt
      supporting_text: Lacks endonuclease activity and does not appear to cleave target mRNAs.
- term:
    id: GO:0005654
    label: nucleoplasm
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-5578742
  review:
    summary: nucleoplasm is a plausible or reported location/context but is not the core functional
      site annotation for AGO1.
    action: KEEP_AS_NON_CORE
    reason: The core location is cytoplasmic RISC/RNP granules; nuclear, membrane, synaptic,
      exosomal, or other compartment annotations should remain non-core unless tied to a specific
      curated mechanism.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0003723
    label: RNA binding
  evidence_type: IDA
  original_reference_id: PMID:23809764
  review:
    summary: RNA binding is too generic for AGO1.
    action: MARK_AS_OVER_ANNOTATED
    reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC
      rather than a broad nucleic-acid or RNA-binding label.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0004521
    label: RNA endonuclease activity
  evidence_type: IDA
  original_reference_id: PMID:23809764
  negated: true
  review:
    summary: This NOT annotation is correct; wild-type AGO1 lacks RNA endonuclease/slicer activity.
    action: ACCEPT
    reason: PMID:23809764 directly supports the negated annotation by showing structural constraints
      that keep wild-type AGO1 from placing a guide-target RNA duplex into the endonuclease active
      site. The NOT evidence should be retained.
    supported_by:
    - reference_id: PMID:23809764
      supporting_text: We predicted that even upon restoration of the catalytic tetrad, hAGO1-cS7
        sterically hinders the placement of a fully paired guide-target RNA duplex into the
        endonuclease active site.
- term:
    id: GO:0003723
    label: RNA binding
  evidence_type: HDA
  original_reference_id: PMID:22681889
  review:
    summary: RNA binding is too generic for AGO1.
    action: MARK_AS_OVER_ANNOTATED
    reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC
      rather than a broad nucleic-acid or RNA-binding label.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-1606561
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-1606682
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-1912362
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-1912363
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-1912364
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-1912366
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-1912367
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-1912368
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-1912406
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-2318752
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-3209151
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-426489
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-426522
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-4518575
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-5687103
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-8935766
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-8937097
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-8937134
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-8938440
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-8938487
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-8938507
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-8944650
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-8944684
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-8944706
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9011958
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9012203
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9012208
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9038545
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9618392
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9618486
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9624925
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9657791
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9858289
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9858309
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9924921
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9925095
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9925158
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9925159
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9925324
  review:
    summary: cytosol is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:22915799
  review:
    summary: Generic protein binding does not capture the specific Argonaute/RISC function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The cited interactions are best interpreted as RISC/cofactor context for
      small-RNA-guided silencing, not as a standalone core function.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:16289642
  review:
    summary: Generic protein binding does not capture the specific Argonaute/RISC function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The cited interactions are best interpreted as RISC/cofactor context for
      small-RNA-guided silencing, not as a standalone core function.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:20616046
  review:
    summary: Generic protein binding does not capture the specific Argonaute/RISC function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The cited interactions are best interpreted as RISC/cofactor context for
      small-RNA-guided silencing, not as a standalone core function.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0005737
    label: cytoplasm
  evidence_type: IDA
  original_reference_id: PMID:20014101
  review:
    summary: cytoplasm is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0016442
    label: RISC complex
  evidence_type: IDA
  original_reference_id: PMID:17932509
  review:
    summary: RISC complex is consistent with AGO1 as a small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0000956
    label: nuclear-transcribed mRNA catabolic process
  evidence_type: IDA
  original_reference_id: PMID:18771919
  review:
    summary: nuclear-transcribed mRNA catabolic process is downstream or context-specific for AGO1.
    action: KEEP_AS_NON_CORE
    reason: This process may be supported in a particular experiment or pathway model, but it is
      peripheral to the core Argonaute/RISC molecular role.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
- term:
    id: GO:0035278
    label: miRNA-mediated gene silencing by inhibition of translation
  evidence_type: IDA
  original_reference_id: PMID:18771919
  review:
    summary: miRNA-mediated gene silencing by inhibition of translation is consistent with AGO1 as a
      small-RNA-loaded Argonaute/RISC effector.
    action: ACCEPT
    reason: The falcon report supports AGO1 function in miRISC-mediated small-RNA-guided repression
      and related RISC complexes or cytoplasmic RNP compartments.
    supported_by:
    - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
      supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
        functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
        through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
references:
- id: GO_REF:0000002
  title: Gene Ontology annotation through association of InterPro records with GO terms
  findings: []
- id: GO_REF:0000033
  title: Annotation inferences using phylogenetic trees
  findings: []
- id: GO_REF:0000052
  title: Gene Ontology annotation based on curation of immunofluorescence data
  findings: []
- id: GO_REF:0000107
  title: Automatic transfer of experimentally verified manual GO annotation data to orthologs using
    Ensembl Compara
  findings: []
- id: GO_REF:0000117
  title: Electronic Gene Ontology annotations created by ARBA machine learning models
  findings: []
- id: GO_REF:0000120
  title: Combined Automated Annotation using Multiple IEA Methods
  findings: []
- id: PMID:12526743
  title: Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C
    translation initiation factors.
  findings: []
- id: PMID:15260970
  title: Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs.
  findings: []
- id: PMID:16289642
  title: Identification of novel argonaute-associated proteins.
  findings: []
- id: PMID:16756390
  title: Translation repression in human cells by microRNA-induced gene silencing requires RCK/p54.
  findings: []
- id: PMID:17891150
  title: A conserved motif in Argonaute-interacting proteins mediates functional interactions
    through the Argonaute PIWI domain.
  findings: []
- id: PMID:17932509
  title: Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human
    cells.
  findings: []
- id: PMID:18482256
  title: Protein microarray analysis identifies human cellular prion protein interactors.
  findings: []
- id: PMID:18771919
  title: Importance of translation and nonnucleolytic ago proteins for on-target RNA interference.
  findings: []
- id: PMID:19167051
  title: Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs.
  findings: []
- id: PMID:19324964
  title: The C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires
    GW182 to mediate silencing.
  findings: []
- id: PMID:19383768
  title: The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence bound transcripts
    independently of Argonaute proteins.
  findings: []
- id: PMID:19716330
  title: Mammalian miRNA RISC recruits CAF1 and PABP to affect PABP-dependent deadenylation.
  findings: []
- id: PMID:19966796
  title: ATP-dependent human RISC assembly pathways.
  findings: []
- id: PMID:20014101
  title: Mouse ZAR1-like (XM_359149) colocalizes with mRNA processing components and its
    dominant-negative mutant caused two-cell-stage embryonic arrest.
  findings: []
- id: PMID:20616046
  title: LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for microRNA-mediated gene
    silencing.
  findings: []
- id: PMID:22484317
  title: Human prion protein binds Argonaute and promotes accumulation of microRNA effector
    complexes.
  findings: []
- id: PMID:22681889
  title: The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
  findings: []
- id: PMID:22795694
  title: Slicing-independent RISC activation requires the argonaute PAZ domain.
  findings: []
- id: PMID:22915799
  title: HIV-1 replication and APOBEC3 antiviral activity are not regulated by P bodies.
  findings: []
- id: PMID:23426184
  title: Hypoxia-responsive miRNAs target argonaute 1 to promote angiogenesis.
  findings: []
- id: PMID:23809764
  title: Eukaryote-specific insertion elements control human ARGONAUTE slicer activity.
  findings: []
- id: PMID:25036637
  title: A quantitative chaperone interaction network reveals the architecture of cellular protein
    homeostasis pathways.
  findings: []
- id: PMID:25336585
  title: Cellular microRNAs up-regulate transcription via interaction with promoter TATA-box motifs.
  findings: []
- id: PMID:28330616
  title: Systematic Analysis of Human Protein Phosphatase Interactions and Dynamics.
  findings: []
- id: PMID:28683311
  title: Argonaute Utilization for miRNA Silencing Is Determined by Phosphorylation-Dependent
    Recruitment of LIM-Domain-Containing Proteins.
  findings: []
- id: PMID:28781232
  title: Multivalent Recruitment of Human Argonaute by GW182.
  findings: []
- id: PMID:33961781
  title: Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
  findings: []
- id: PMID:35271311
  title: 'OpenCell: Endogenous tagging for the cartography of human cellular organization.'
  findings: []
- id: PMID:35709258
  title: Spatial centrosome proteome of human neural cells uncovers disease-relevant heterogeneity.
  findings: []
- id: PMID:40205054
  title: Multimodal cell maps as a foundation for structural and functional genomics.
  findings: []
- id: Reactome:R-HSA-1606561
  title: MIR449 microRNAs bind 3'UTR of NOTCH1 mRNA
  findings: []
- id: Reactome:R-HSA-1606682
  title: MIR34 microRNAs bind 3'UTR of NOTCH1 mRNA
  findings: []
- id: Reactome:R-HSA-1912362
  title: MIR150 microRNA binds 3'UTR of NOTCH3 mRNA
  findings: []
- id: Reactome:R-HSA-1912363
  title: MIR200B/C microRNAs bind NOTCH1 mRNA
  findings: []
- id: Reactome:R-HSA-1912364
  title: MIR181C microRNA binds 3'UTR of NOTCH4 mRNA
  findings: []
- id: Reactome:R-HSA-1912366
  title: MIR206 microRNA binds 3'UTR of NOTCH3 mRNA
  findings: []
- id: Reactome:R-HSA-1912367
  title: MIR34 microRNAs bind 3'UTR of NOTCH2 mRNA
  findings: []
- id: Reactome:R-HSA-1912368
  title: MIR302A microRNA binds 3'UTR of NOTCH4 mRNA
  findings: []
- id: Reactome:R-HSA-1912406
  title: p53 positively regulates transcription of MIR34 microRNAs
  findings: []
- id: Reactome:R-HSA-2318752
  title: miR-26A microRNAs bind PTEN mRNA
  findings: []
- id: Reactome:R-HSA-3209151
  title: miR-24 binds p16INK4A and p14ARF mRNAs
  findings: []
- id: Reactome:R-HSA-426489
  title: RISC binds inexactly matching target RNAs
  findings: []
- id: Reactome:R-HSA-426522
  title: Nonendonucleolytic RISC binds exactly matching target RNAs
  findings: []
- id: Reactome:R-HSA-4518575
  title: miR-92b binds 3'UTR of NLK mRNA
  findings: []
- id: Reactome:R-HSA-5578742
  title: AGO1,2:small RNA complexes interact with chromatin
  findings: []
- id: Reactome:R-HSA-5687103
  title: FOXO3 regulates MIR34B,C expression
  findings: []
- id: Reactome:R-HSA-8935766
  title: miR-378 binds RUNX1 mRNA
  findings: []
- id: Reactome:R-HSA-8937097
  title: MIR27A gene transcription is stimulated by the complex containing RUNX1, PRMT1 and GATA1
    and inhibited by the complex of RUNX1, SIN3A and PRMT6
  findings: []
- id: Reactome:R-HSA-8937134
  title: miR-27a binds RUNX1 mRNA
  findings: []
- id: Reactome:R-HSA-8938440
  title: miR-17 binds RUNX1 mRNA
  findings: []
- id: Reactome:R-HSA-8938487
  title: miR-20a binds RUNX1 mRNA
  findings: []
- id: Reactome:R-HSA-8938507
  title: miR-106a binds RUNX1 mRNA
  findings: []
- id: Reactome:R-HSA-8944650
  title: miR-214 microRNA binds PTEN mRNA
  findings: []
- id: Reactome:R-HSA-8944684
  title: miR-205 microRNA binds PTEN mRNA
  findings: []
- id: Reactome:R-HSA-8944706
  title: miR-21 nonendonucleolytic RISC binds PTEN mRNA
  findings: []
- id: Reactome:R-HSA-9011958
  title: miR-26A and B bind to the 3'UTR of the GREB1 mRNA
  findings: []
- id: Reactome:R-HSA-9012203
  title: miR-26A and B bind to the 3'UTR of the CHD11 mRNA
  findings: []
- id: Reactome:R-HSA-9012208
  title: miR-26A and B bind to the 3'UTR of the KPNA2 mRNA
  findings: []
- id: Reactome:R-HSA-9038545
  title: miR-613 binds to the 3'UTR of the NR1H3 mRNA
  findings: []
- id: Reactome:R-HSA-9618392
  title: miR-26 binds to the 3'UTR of the ARL4C mRNA
  findings: []
- id: Reactome:R-HSA-9618486
  title: miR-26 binds to the 3'UTR of the ABCA1 mRNA
  findings: []
- id: Reactome:R-HSA-9624925
  title: miR-33 binds to the 3'UTR of the ABCA1 mRNA
  findings: []
- id: Reactome:R-HSA-9657791
  title: miR-144 binds to the 3'UTR of the ABCA1 mRNA
  findings: []
- id: Reactome:R-HSA-9858289
  title: MITF-M-dependent miR-211 expression
  findings: []
- id: Reactome:R-HSA-9858309
  title: miR-211 RISC binds POU3F2 mRNA
  findings: []
- id: Reactome:R-HSA-9924921
  title: CD274 mRNA binds miR-34 RISC
  findings: []
- id: Reactome:R-HSA-9925095
  title: CD274 mRNA binds miR-340 RISC
  findings: []
- id: Reactome:R-HSA-9925158
  title: CD274 mRNA binds miR-152 RISC
  findings: []
- id: Reactome:R-HSA-9925159
  title: CD274 mRNA binds miR-140 RISC
  findings: []
- id: Reactome:R-HSA-9925324
  title: CD274 mRNA binds miR-200B/C RISC
  findings: []
- id: file:human/AGO1/AGO1-uniprot.txt
  title: UniProt text export for AGO1
  findings:
  - statement: UniProt identifies AGO1 and its protein family/domain context.
    supporting_text: Belongs to the argonaute family. Ago subfamily.
- id: file:human/AGO1/AGO1-deep-research-falcon.md
  title: Falcon deep research report for AGO1
  findings:
  - statement: Falcon research supports the reviewed core function of AGO1.
    supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
      functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
      through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
core_functions:
- description: Small-RNA-guided post-transcriptional repression as a non-slicer Argonaute component
    of miRISC.
  molecular_function:
    id: GO:0035198
    label: miRNA binding
  directly_involved_in:
  - id: GO:0035194
    label: regulatory ncRNA-mediated post-transcriptional gene silencing
  - id: GO:0035278
    label: miRNA-mediated gene silencing by inhibition of translation
  in_complex:
    id: GO:0016442
    label: RISC complex
  locations:
  - id: GO:0005737
    label: cytoplasm
  - id: GO:0000932
    label: P-body
  supported_by:
  - reference_id: file:human/AGO1/AGO1-deep-research-falcon.md
    supporting_text: Human **AGO1 (EIF2C1; UniProt Q9UL18)** is a **non-slicer Argonaute** that
      functions as a core miRISC effector mediating **miRNA-guided repression and mRNA decay**
      through recruitment of **TNRC6/GW182** and downstream **deadenylation/decapping** pathways.
proposed_new_terms: []
suggested_questions:
- question: Which endogenous human tissues or stress states require AGO1-specific RISC activity
    rather than redundant activity from other AGO paralogs?
- question: Which protein cofactors determine guide loading, localization, and non-core nuclear or
    granule-associated functions of AGO1?
suggested_experiments:
- description: Endogenous tagging of AGO1 followed by small-RNA CLIP, target RNA profiling, and
    paralog-specific rescue in AGO-depleted human cells.
  experiment_type: genome editing/RNA-protein interaction profiling
  hypothesis: AGO1 has paralog-specific guide, target, and compartment preferences within human RISC
    biology.
- description: Biochemical reconstitution of AGO1-RISC with representative miRNA and siRNA guides to
    compare target repression, target cleavage, and TNRC6 recruitment.
  experiment_type: biochemical reconstitution
  hypothesis: AGO1 activity is best explained by guide-loaded RISC function rather than generic RNA
    or protein binding.

Gene Description

Existing Annotations Review

Core Functions

Small-RNA-guided post-transcriptional repression as a non-slicer Argonaute component of miRISC.

References

Suggested Questions for Experts

Suggested Experiments

Deep Research

Falcon

Comprehensive Research Report: Human AGO1 (EIF2C1) — Functional Annotation (UniProt Q9UL18)

1) Target verification (mandatory identity check)

2) Key concepts and definitions (current understanding)

2.1 Argonaute proteins and miRISC

2.2 Domain architecture and what the domains do

2.3 Enzymatic/catalytic function (substrate specificity)

3) Molecular function and mechanisms (pathways and partners)

3.1 RISC loading and maturation partners

3.2 Downstream repression: deadenylation, decapping, and decay

4) Subcellular localization (where AGO1 acts) and regulation

4.1 Cytoplasmic gene silencing with partitioning into RNA granules

4.2 Post-translational modifications (PTMs) that modulate localization and activity

4.3 RNP remodeling and polysome/P-body partitioning

4.4 ER-associated condensates

4.5 AGO1 isoform: translational readthrough product (AGO1x)

5) Recent developments and latest research (prioritizing 2023–2024)

5.1 Human genetics: AGO1 as a neurodevelopmental disorder gene

5.2 Functional interpretation via cross-species modeling

6) Current applications and real-world implementations

6.1 RNAi/miRNA technologies (mechanism-based implementation)

6.2 Practical research implementations (assays and omics)

7) Expert opinions and authoritative analyses (interpretation)

7.1 Mechanistic consensus: AGO1 is a non-slicer effector specialized for repression/decay

7.2 Regulation is multi-layered and context dependent

8) Relevant statistics and data points (from recent studies and databases)

9) Limitations of the retrieved corpus (important for correctness)

Summary (functional annotation)

Citations

📄 View Raw YAML