AGO2 encodes human Argonaute-2, the principal catalytic Argonaute in mammalian RNA silencing. AGO2 binds miRNA or siRNA guides in RISC and can repress targets non-catalytically through miRISC effector recruitment or cleave highly complementary RNA targets as an RNA-guided endoribonuclease.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
|
GO:0005634
nucleus
|
IBA
GO_REF:0000033 |
KEEP AS NON CORE |
Summary: nucleus is a plausible or reported location/context but is not the core functional site annotation for AGO2.
Reason: The core location is cytoplasmic RISC/RNP granules; nuclear, membrane, synaptic, exosomal, or other compartment annotations should remain non-core unless tied to a specific curated mechanism.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005737
cytoplasm
|
IBA
GO_REF:0000033 |
ACCEPT |
Summary: cytoplasm is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0004521
RNA endonuclease activity
|
IBA
GO_REF:0000033 |
ACCEPT |
Summary: RNA endonuclease activity is appropriate for AGO2 catalytic slicer activity.
Reason: AGO2 is the principal human slicer Argonaute and cleaves guide-complementary target RNA in RISC.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0035194
regulatory ncRNA-mediated post-transcriptional gene silencing
|
IBA
GO_REF:0000033 |
ACCEPT |
Summary: regulatory ncRNA-mediated post-transcriptional gene silencing is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0016442
RISC complex
|
IBA
GO_REF:0000033 |
ACCEPT |
Summary: RISC complex is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0036464
cytoplasmic ribonucleoprotein granule
|
IBA
GO_REF:0000033 |
ACCEPT |
Summary: cytoplasmic ribonucleoprotein granule is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0031054
pre-miRNA processing
|
IBA
GO_REF:0000033 |
ACCEPT |
Summary: pre-miRNA processing is supported for AGO2 because AGO2 participates in RISC maturation and AGO2-specific pre-miRNA handling such as pre-miR-451 biology.
Reason: AGO2 is the catalytic Argonaute and has specific evidence for guide loading, RISC maturation, and pre-miRNA-linked processing contexts.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0035198
miRNA binding
|
IBA
GO_REF:0000033 |
ACCEPT |
Summary: miRNA binding is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0003727
single-stranded RNA binding
|
IBA
GO_REF:0000033 |
MARK AS OVER ANNOTATED |
Summary: single-stranded RNA binding is too generic for AGO2.
Reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC rather than a broad nucleic-acid or RNA-binding label.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0000340
RNA 7-methylguanosine cap binding
|
IEA
GO_REF:0000104 |
KEEP AS NON CORE |
Summary: RNA 7-methylguanosine cap binding is experimentally supported for AGO2-mediated translational repression, but is not the main AGO2 slicer/RISC function.
Reason: This is a specific cap-binding activity supported by PMID:17524464 and UniProt, best retained as a non-core translational-repression mechanism.
Supporting Evidence:
PMID:17524464
We identify conserved aromatic residues within the MC motif of human Ago2 that are required for binding to the m(7)G cap and for translational repression but do not affect the assembly of Ago2 with miRNA or its catalytic activity.
file:human/AGO2/AGO2-uniprot.txt
May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E.
|
|
GO:0000932
P-body
|
IEA
GO_REF:0000120 |
ACCEPT |
Summary: P-body is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0003676
nucleic acid binding
|
IEA
GO_REF:0000002 |
MARK AS OVER ANNOTATED |
Summary: nucleic acid binding is too generic for AGO2.
Reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC rather than a broad nucleic-acid or RNA-binding label.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0003723
RNA binding
|
IEA
GO_REF:0000120 |
MARK AS OVER ANNOTATED |
Summary: RNA binding is too generic for AGO2.
Reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC rather than a broad nucleic-acid or RNA-binding label.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0003725
double-stranded RNA binding
|
IEA
GO_REF:0000117 |
MARK AS OVER ANNOTATED |
Summary: double-stranded RNA binding is too generic for AGO2.
Reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC rather than a broad nucleic-acid or RNA-binding label.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0003727
single-stranded RNA binding
|
IEA
GO_REF:0000117 |
MARK AS OVER ANNOTATED |
Summary: single-stranded RNA binding is too generic for AGO2.
Reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC rather than a broad nucleic-acid or RNA-binding label.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005634
nucleus
|
IEA
GO_REF:0000120 |
KEEP AS NON CORE |
Summary: nucleus is a plausible or reported location/context but is not the core functional site annotation for AGO2.
Reason: The core location is cytoplasmic RISC/RNP granules; nuclear, membrane, synaptic, exosomal, or other compartment annotations should remain non-core unless tied to a specific curated mechanism.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0006355
regulation of DNA-templated transcription
|
IEA
GO_REF:0000002 |
KEEP AS NON CORE |
Summary: regulation of DNA-templated transcription reflects a reported nuclear or regulatory context rather than the main conserved AGO2 function.
Reason: Argonaute paralogs have reported nuclear/regulatory interactions, but the dominant conserved function remains small-RNA-guided post-transcriptional repression.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0006413
translational initiation
|
IEA
GO_REF:0000108 |
REMOVE |
Summary: translational initiation is a legacy implication of the EIF2C name and is not supported as an Argonaute activity.
Reason: Human Argonautes are RISC effector proteins, not translation initiation factors in the canonical eIF sense.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0016442
RISC complex
|
IEA
GO_REF:0000120 |
ACCEPT |
Summary: RISC complex is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0016891
RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism
|
IEA
GO_REF:0000104 |
ACCEPT |
Summary: RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism is appropriate for AGO2 catalytic slicer activity.
Reason: AGO2 is the principal human slicer Argonaute and cleaves guide-complementary target RNA in RISC.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0031047
regulatory ncRNA-mediated gene silencing
|
IEA
GO_REF:0000120 |
ACCEPT |
Summary: regulatory ncRNA-mediated gene silencing is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0031054
pre-miRNA processing
|
IEA
GO_REF:0000120 |
ACCEPT |
Summary: pre-miRNA processing is supported for AGO2 because AGO2 participates in RISC maturation and AGO2-specific pre-miRNA handling such as pre-miR-451 biology.
Reason: AGO2 is the catalytic Argonaute and has specific evidence for guide loading, RISC maturation, and pre-miRNA-linked processing contexts.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0035197
siRNA binding
|
IEA
GO_REF:0000104 |
ACCEPT |
Summary: siRNA binding is a core component of AGO2 RISC loading and siRNA-guided cleavage.
Reason: AGO2 forms minimal RISC with short guide RNAs including siRNAs; siRNA guide binding is central to AGO2's RNAi/slicer activity.
Supporting Evidence:
file:human/AGO2/AGO2-uniprot.txt
The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA).
|
|
GO:0035198
miRNA binding
|
IEA
GO_REF:0000120 |
ACCEPT |
Summary: miRNA binding is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0035278
miRNA-mediated gene silencing by inhibition of translation
|
IEA
GO_REF:0000120 |
ACCEPT |
Summary: miRNA-mediated gene silencing by inhibition of translation is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0035279
miRNA-mediated gene silencing by mRNA destabilization
|
IEA
GO_REF:0000120 |
ACCEPT |
Summary: miRNA-mediated gene silencing by mRNA destabilization is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0036464
cytoplasmic ribonucleoprotein granule
|
IEA
GO_REF:0000117 |
ACCEPT |
Summary: cytoplasmic ribonucleoprotein granule is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0045947
negative regulation of translational initiation
|
IEA
GO_REF:0000104 |
KEEP AS NON CORE |
Summary: negative regulation of translational initiation is downstream or context-specific for AGO2.
Reason: This process may be supported in a particular experiment or pathway model, but it is peripheral to the core Argonaute/RISC molecular role.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0070551
endoribonuclease activity, cleaving siRNA-paired mRNA
|
IEA
GO_REF:0000002 |
ACCEPT |
Summary: endoribonuclease activity, cleaving siRNA-paired mRNA is appropriate for AGO2 catalytic slicer activity.
Reason: AGO2 is the principal human slicer Argonaute and cleaves guide-complementary target RNA in RISC.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0070922
RISC complex assembly
|
IEA
GO_REF:0000117 |
ACCEPT |
Summary: RISC complex assembly is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0098808
mRNA cap binding
|
IEA
GO_REF:0000104 |
KEEP AS NON CORE |
Summary: mRNA cap binding is experimentally supported for AGO2-mediated translational repression, but is not its central guide-loaded slicer/RISC activity.
Reason: PMID:17524464 and UniProt support AGO2 binding to the mRNA m7G cap as a mechanism for translational repression. This is a specific non-core molecular function rather than a generic RNA-binding over-annotation.
Supporting Evidence:
PMID:17524464
We identify conserved aromatic residues within the MC motif of human Ago2 that are required for binding to the m(7)G cap and for translational repression but do not affect the assembly of Ago2 with miRNA or its catalytic activity.
file:human/AGO2/AGO2-uniprot.txt
May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E.
|
|
GO:0005515
protein binding
|
IPI
PMID:14749716 Characterization of the interactions between mammalian PAZ P... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:16424907 The role of PACT in the RNA silencing pathway. |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:16756390 Translation repression in human cells by microRNA-induced ge... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:17400507 Identification of potential protein interactors of Lrrk2. |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:17891150 A conserved motif in Argonaute-interacting proteins mediates... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:17932509 Proteomic and functional analysis of Argonaute-containing mR... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:18178619 In vitro reconstitution of the human RISC-loading complex. |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:19167051 Importin 8 is a gene silencing factor that targets argonaute... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:19324964 The C-terminal half of human Ago2 binds to multiple GW-rich ... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:19383768 The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C s... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:19716330 Mammalian miRNA RISC recruits CAF1 and PABP to affect PABP-d... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:19820710 Structural insights into RNA processing by the human RISC-lo... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:19838187 Ago-TNRC6 triggers microRNA-mediated decay by promoting two ... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:19955415 CRM1 mediates nuclear-cytoplasmic shuttling of mature microR... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:20671708 Pathogenic LRRK2 negatively regulates microRNA-mediated tran... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:21525958 The ribosomal protein RACK1 is required for microRNA functio... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:21840310 Ago2/miRISC-mediated inhibition of CBP80/20-dependent transl... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:21858095 Nuclear pore complex protein mediated nuclear localization o... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:21903422 Mapping a dynamic innate immunity protein interaction networ... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:21981923 GW182 proteins directly recruit cytoplasmic deadenylase comp... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:22366686 Senescence is an endogenous trigger for microRNA-directed tr... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:22484317 Human prion protein binds Argonaute and promotes accumulatio... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:23085987 Wig1 prevents cellular senescence by regulating p21 mRNA dec... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:23090477 Defining a new role of GW182 in maintaining miRNA stability. |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:23622242 ADAR1 forms a complex with Dicer to promote microRNA process... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:23636329 EGFR modulates microRNA maturation in response to hypoxia th... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:23661684 Differential roles of human Dicer-binding proteins TRBP and ... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:23741051 Hsp90 cochaperones p23 and FKBP4 physically interact with hA... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:24043833 Structural features of Argonaute-GW182 protein interactions. |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:24550003 Involvement of telomerase reverse transcriptase in heterochr... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:24981741 MTDH-SND1 interaction is crucial for expansion and activity ... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:25446899 Cancer exosomes perform cell-independent microRNA biogenesis... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:26496610 A human interactome in three quantitative dimensions organiz... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:28683311 Argonaute Utilization for miRNA Silencing Is Determined by P... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:28877994 Cadherin complexes recruit mRNAs and RISC to regulate epithe... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:39251607 Systematic identification of post-transcriptional regulatory... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0003729
mRNA binding
|
IEA
GO_REF:0000107 |
MARK AS OVER ANNOTATED |
Summary: mRNA binding is too generic for AGO2.
Reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC rather than a broad nucleic-acid or RNA-binding label.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0004521
RNA endonuclease activity
|
IEA
GO_REF:0000107 |
ACCEPT |
Summary: RNA endonuclease activity is appropriate for AGO2 catalytic slicer activity.
Reason: AGO2 is the principal human slicer Argonaute and cleaves guide-complementary target RNA in RISC.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
IEA
GO_REF:0000107 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0030425
dendrite
|
IEA
GO_REF:0000107 |
KEEP AS NON CORE |
Summary: dendrite is a plausible or reported location/context but is not the core functional site annotation for AGO2.
Reason: The core location is cytoplasmic RISC/RNP granules; nuclear, membrane, synaptic, exosomal, or other compartment annotations should remain non-core unless tied to a specific curated mechanism.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0060213
positive regulation of nuclear-transcribed mRNA poly(A) tail shortening
|
IEA
GO_REF:0000107 |
KEEP AS NON CORE |
Summary: positive regulation of nuclear-transcribed mRNA poly(A) tail shortening is downstream or context-specific for AGO2.
Reason: This process may be supported in a particular experiment or pathway model, but it is peripheral to the core Argonaute/RISC molecular role.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0070578
RISC-loading complex
|
IEA
GO_REF:0000120 |
ACCEPT |
Summary: RISC-loading complex is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:1900153
positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
|
IEA
GO_REF:0000107 |
KEEP AS NON CORE |
Summary: positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay is downstream or context-specific for AGO2.
Reason: This process may be supported in a particular experiment or pathway model, but it is peripheral to the core Argonaute/RISC molecular role.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:1990904
ribonucleoprotein complex
|
IEA
GO_REF:0000107 |
KEEP AS NON CORE |
Summary: ribonucleoprotein complex is a plausible or reported location/context but is not the core functional site annotation for AGO2.
Reason: The core location is cytoplasmic RISC/RNP granules; nuclear, membrane, synaptic, exosomal, or other compartment annotations should remain non-core unless tied to a specific curated mechanism.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0035196
miRNA processing
|
NAS
PMID:28781232 Multivalent Recruitment of Human Argonaute by GW182. |
ACCEPT |
Summary: miRNA processing is supported for AGO2 because AGO2 participates in RISC maturation and AGO2-specific pre-miRNA handling such as pre-miR-451 biology.
Reason: AGO2 is the catalytic Argonaute and has specific evidence for guide loading, RISC maturation, and pre-miRNA-linked processing contexts.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0035194
regulatory ncRNA-mediated post-transcriptional gene silencing
|
TAS
Reactome:R-HSA-426496 |
ACCEPT |
Summary: regulatory ncRNA-mediated post-transcriptional gene silencing is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0036464
cytoplasmic ribonucleoprotein granule
|
IDA
GO_REF:0000052 |
ACCEPT |
Summary: cytoplasmic ribonucleoprotein granule is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0004521
RNA endonuclease activity
|
TAS
Reactome:R-HSA-426520 |
ACCEPT |
Summary: RNA endonuclease activity is appropriate for AGO2 catalytic slicer activity.
Reason: AGO2 is the principal human slicer Argonaute and cleaves guide-complementary target RNA in RISC.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0090128
regulation of synapse maturation
|
IDA
PMID:29735530 NMDA receptor-dependent dephosphorylation of serine 387 in A... |
KEEP AS NON CORE |
Summary: regulation of synapse maturation is downstream or context-specific for AGO2.
Reason: This process may be supported in a particular experiment or pathway model, but it is peripheral to the core Argonaute/RISC molecular role.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0090128
regulation of synapse maturation
|
IMP
PMID:29735530 NMDA receptor-dependent dephosphorylation of serine 387 in A... |
KEEP AS NON CORE |
Summary: regulation of synapse maturation is downstream or context-specific for AGO2.
Reason: This process may be supported in a particular experiment or pathway model, but it is peripheral to the core Argonaute/RISC molecular role.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0098794
postsynapse
|
IDA
PMID:29735530 NMDA receptor-dependent dephosphorylation of serine 387 in A... |
KEEP AS NON CORE |
Summary: postsynapse is a plausible or reported location/context but is not the core functional site annotation for AGO2.
Reason: The core location is cytoplasmic RISC/RNP granules; nuclear, membrane, synaptic, exosomal, or other compartment annotations should remain non-core unless tied to a specific curated mechanism.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0098794
postsynapse
|
IMP
PMID:29735530 NMDA receptor-dependent dephosphorylation of serine 387 in A... |
KEEP AS NON CORE |
Summary: postsynapse is a plausible or reported location/context but is not the core functional site annotation for AGO2.
Reason: The core location is cytoplasmic RISC/RNP granules; nuclear, membrane, synaptic, exosomal, or other compartment annotations should remain non-core unless tied to a specific curated mechanism.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0098978
glutamatergic synapse
|
IDA
PMID:29735530 NMDA receptor-dependent dephosphorylation of serine 387 in A... |
KEEP AS NON CORE |
Summary: glutamatergic synapse is a plausible or reported location/context but is not the core functional site annotation for AGO2.
Reason: The core location is cytoplasmic RISC/RNP granules; nuclear, membrane, synaptic, exosomal, or other compartment annotations should remain non-core unless tied to a specific curated mechanism.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0098978
glutamatergic synapse
|
IMP
PMID:29735530 NMDA receptor-dependent dephosphorylation of serine 387 in A... |
KEEP AS NON CORE |
Summary: glutamatergic synapse is a plausible or reported location/context but is not the core functional site annotation for AGO2.
Reason: The core location is cytoplasmic RISC/RNP granules; nuclear, membrane, synaptic, exosomal, or other compartment annotations should remain non-core unless tied to a specific curated mechanism.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0098808
mRNA cap binding
|
IDA
PMID:17524464 An mRNA m7G cap binding-like motif within human Ago2 repress... |
KEEP AS NON CORE |
Summary: mRNA cap binding is experimentally supported for AGO2-mediated translational repression, but is not its central guide-loaded slicer/RISC activity.
Reason: PMID:17524464 and UniProt support AGO2 binding to the mRNA m7G cap as a mechanism for translational repression. This is a specific non-core molecular function rather than a generic RNA-binding over-annotation.
Supporting Evidence:
PMID:17524464
We identify conserved aromatic residues within the MC motif of human Ago2 that are required for binding to the m(7)G cap and for translational repression but do not affect the assembly of Ago2 with miRNA or its catalytic activity.
file:human/AGO2/AGO2-uniprot.txt
May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E.
|
|
GO:0005737
cytoplasm
|
IDA
PMID:17932509 Proteomic and functional analysis of Argonaute-containing mR... |
ACCEPT |
Summary: cytoplasm is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0033962
P-body assembly
|
IDA
PMID:31400113 KSHV RNA-binding protein ORF57 inhibits P-body formation to ... |
KEEP AS NON CORE |
Summary: P-body assembly is downstream or context-specific for AGO2.
Reason: This process may be supported in a particular experiment or pathway model, but it is peripheral to the core Argonaute/RISC molecular role.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0035925
mRNA 3'-UTR AU-rich region binding
|
IDA
PMID:17382880 AU-rich-element-mediated upregulation of translation by FXR1... |
MARK AS OVER ANNOTATED |
Summary: mRNA 3'-UTR AU-rich region binding is too generic for AGO2.
Reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC rather than a broad nucleic-acid or RNA-binding label.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0045727
positive regulation of translation
|
IDA
PMID:17382880 AU-rich-element-mediated upregulation of translation by FXR1... |
KEEP AS NON CORE |
Summary: positive regulation of translation is downstream or context-specific for AGO2.
Reason: This process may be supported in a particular experiment or pathway model, but it is peripheral to the core Argonaute/RISC molecular role.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005654
nucleoplasm
|
TAS
Reactome:R-HSA-5578742 |
KEEP AS NON CORE |
Summary: nucleoplasm is a plausible or reported location/context but is not the core functional site annotation for AGO2.
Reason: The core location is cytoplasmic RISC/RNP granules; nuclear, membrane, synaptic, exosomal, or other compartment annotations should remain non-core unless tied to a specific curated mechanism.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005654
nucleoplasm
|
TAS
Reactome:R-HSA-5578744 |
KEEP AS NON CORE |
Summary: nucleoplasm is a plausible or reported location/context but is not the core functional site annotation for AGO2.
Reason: The core location is cytoplasmic RISC/RNP granules; nuclear, membrane, synaptic, exosomal, or other compartment annotations should remain non-core unless tied to a specific curated mechanism.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005654
nucleoplasm
|
TAS
Reactome:R-HSA-5578966 |
KEEP AS NON CORE |
Summary: nucleoplasm is a plausible or reported location/context but is not the core functional site annotation for AGO2.
Reason: The core location is cytoplasmic RISC/RNP granules; nuclear, membrane, synaptic, exosomal, or other compartment annotations should remain non-core unless tied to a specific curated mechanism.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0035278
miRNA-mediated gene silencing by inhibition of translation
|
IDA
PMID:23409027 eIF4GI facilitates the MicroRNA-mediated gene silencing. |
ACCEPT |
Summary: miRNA-mediated gene silencing by inhibition of translation is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0016442
RISC complex
|
IDA
PMID:19701182 An RNA-dependent RNA polymerase formed by TERT and the RMRP ... |
ACCEPT |
Summary: RISC complex is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0070922
RISC complex assembly
|
IDA
PMID:19701182 An RNA-dependent RNA polymerase formed by TERT and the RMRP ... |
ACCEPT |
Summary: RISC complex assembly is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:29395067 High-Density Proximity Mapping Reveals the Subcellular Organ... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0016442
RISC complex
|
IGI
PMID:31507089 Reciprocal regulation of miR-206 and IL-6/STAT3 pathway medi... |
ACCEPT |
Summary: RISC complex is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0035198
miRNA binding
|
IPI
PMID:31507089 Reciprocal regulation of miR-206 and IL-6/STAT3 pathway medi... |
ACCEPT |
Summary: miRNA binding is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0016442
RISC complex
|
IGI
PMID:31012336 MicroRNA-31 Negatively Regulates Interleukin-34 Expression I... |
ACCEPT |
Summary: RISC complex is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0035198
miRNA binding
|
IPI
PMID:31012336 MicroRNA-31 Negatively Regulates Interleukin-34 Expression I... |
ACCEPT |
Summary: miRNA binding is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:26009488 Clnk plays a role in TNF-alpha-induced cell death in murine ... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0016442
RISC complex
|
IPI
PMID:19826008 An integrated approach for experimental target identificatio... |
ACCEPT |
Summary: RISC complex is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005634
nucleus
|
IDA
PMID:23985560 microRNA-9 targets the long non-coding RNA MALAT1 for degrad... |
KEEP AS NON CORE |
Summary: nucleus is a plausible or reported location/context but is not the core functional site annotation for AGO2.
Reason: The core location is cytoplasmic RISC/RNP granules; nuclear, membrane, synaptic, exosomal, or other compartment annotations should remain non-core unless tied to a specific curated mechanism.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0042985
negative regulation of amyloid precursor protein biosynthetic process
|
ISS
GO_REF:0000024 |
KEEP AS NON CORE |
Summary: negative regulation of amyloid precursor protein biosynthetic process is downstream or context-specific for AGO2.
Reason: This process may be supported in a particular experiment or pathway model, but it is peripheral to the core Argonaute/RISC molecular role.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:24726324 MOV10 Is a 5' to 3' RNA helicase contributing to UPF1 mRNA t... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-1606561 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-1606682 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-1912362 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-1912363 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-1912364 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-1912366 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-1912367 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-1912368 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-1912406 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-203862 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-2106614 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-2106615 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-2106625 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-210805 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-2318752 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-426464 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-426489 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-426520 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-4518575 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-5578712 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-5578744 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-5578965 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-5578966 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-5687103 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-8935864 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-8935930 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-8936058 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-8936068 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-8939129 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-8944483 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-8944522 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-8944538 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-8944569 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-8944599 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-8944632 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-8944684 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-8945709 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-8948524 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-8948536 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-8948569 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-8948582 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-8948583 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-8948594 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-8948602 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-8948612 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-8948621 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-8948623 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-8948641 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-8948651 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9011958 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9012203 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9012208 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9023909 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9023912 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9038545 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9618392 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9618486 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9624925 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9657791 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9759950 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9768616 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9768643 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9768788 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9860139 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9860160 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9860168 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9924908 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9924921 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9924968 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9925087 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9925095 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9925158 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9925159 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9925246 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9925270 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9925289 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9925320 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005829
cytosol
|
TAS
Reactome:R-HSA-9925324 |
ACCEPT |
Summary: cytosol is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:28546213 Tudor-SN-mediated endonucleolytic decay of human cell microR... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:25697406 Roquin binds microRNA-146a and Argonaute2 to regulate microR... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0045766
positive regulation of angiogenesis
|
IDA
PMID:27208409 miR-15b-AGO2 play a critical role in HTR8/SVneo invasion and... |
KEEP AS NON CORE |
Summary: positive regulation of angiogenesis is downstream or context-specific for AGO2.
Reason: This process may be supported in a particular experiment or pathway model, but it is peripheral to the core Argonaute/RISC molecular role.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:1901165
positive regulation of trophoblast cell migration
|
IMP
PMID:27208409 miR-15b-AGO2 play a critical role in HTR8/SVneo invasion and... |
KEEP AS NON CORE |
Summary: positive regulation of trophoblast cell migration is downstream or context-specific for AGO2.
Reason: This process may be supported in a particular experiment or pathway model, but it is peripheral to the core Argonaute/RISC molecular role.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0000932
P-body
|
IDA
PMID:23125361 The mammalian TRIM-NHL protein TRIM71/LIN-41 is a repressor ... |
ACCEPT |
Summary: P-body is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:23125361 The mammalian TRIM-NHL protein TRIM71/LIN-41 is a repressor ... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0016442
RISC complex
|
IDA
PMID:17531811 RNA helicase A interacts with RISC in human cells and functi... |
ACCEPT |
Summary: RISC complex is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0070578
RISC-loading complex
|
IDA
PMID:17531811 RNA helicase A interacts with RISC in human cells and functi... |
ACCEPT |
Summary: RISC-loading complex is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:17531811 RNA helicase A interacts with RISC in human cells and functi... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:23361462 Structural insights into RISC assembly facilitated by dsRNA-... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0070062
extracellular exosome
|
IDA
PMID:28159509 Human Pericardial Fluid Contains Exosomes Enriched with Card... |
KEEP AS NON CORE |
Summary: extracellular exosome is a plausible or reported location/context but is not the core functional site annotation for AGO2.
Reason: The core location is cytoplasmic RISC/RNP granules; nuclear, membrane, synaptic, exosomal, or other compartment annotations should remain non-core unless tied to a specific curated mechanism.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0031054
pre-miRNA processing
|
IDA
PMID:16424907 The role of PACT in the RNA silencing pathway. |
ACCEPT |
Summary: pre-miRNA processing is supported for AGO2 because AGO2 participates in RISC maturation and AGO2-specific pre-miRNA handling such as pre-miR-451 biology.
Reason: AGO2 is the catalytic Argonaute and has specific evidence for guide loading, RISC maturation, and pre-miRNA-linked processing contexts.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0070578
RISC-loading complex
|
IDA
PMID:23661684 Differential roles of human Dicer-binding proteins TRBP and ... |
ACCEPT |
Summary: RISC-loading complex is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:24862572 Mammalian microtubule P-body dynamics are mediated by nespri... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:27157137 Post-transcriptional gene silencing activity of human GIGYF2... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:23409027 eIF4GI facilitates the MicroRNA-mediated gene silencing. |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0098808
mRNA cap binding
|
IPI
PMID:23409027 eIF4GI facilitates the MicroRNA-mediated gene silencing. |
KEEP AS NON CORE |
Summary: mRNA cap binding is experimentally supported for AGO2-mediated translational repression, but is not its central guide-loaded slicer/RISC activity.
Reason: PMID:17524464 and UniProt support AGO2 binding to the mRNA m7G cap as a mechanism for translational repression. This is a specific non-core molecular function rather than a generic RNA-binding over-annotation.
Supporting Evidence:
PMID:17524464
We identify conserved aromatic residues within the MC motif of human Ago2 that are required for binding to the m(7)G cap and for translational repression but do not affect the assembly of Ago2 with miRNA or its catalytic activity.
file:human/AGO2/AGO2-uniprot.txt
May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E.
|
|
GO:0005515
protein binding
|
IPI
PMID:15766526 Involvement of microRNA in AU-rich element-mediated mRNA ins... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:17507929 MicroRNA silencing through RISC recruitment of eIF6. |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0045944
positive regulation of transcription by RNA polymerase II
|
IMP
PMID:25336585 Cellular microRNAs up-regulate transcription via interaction... |
KEEP AS NON CORE |
Summary: positive regulation of transcription by RNA polymerase II reflects a reported nuclear or regulatory context rather than the main conserved AGO2 function.
Reason: Argonaute paralogs have reported nuclear/regulatory interactions, but the dominant conserved function remains small-RNA-guided post-transcriptional repression.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0035198
miRNA binding
|
TAS
PMID:19820710 Structural insights into RNA processing by the human RISC-lo... |
ACCEPT |
Summary: miRNA binding is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0070578
RISC-loading complex
|
IDA
PMID:19820710 Structural insights into RNA processing by the human RISC-lo... |
ACCEPT |
Summary: RISC-loading complex is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0070578
RISC-loading complex
|
IDA
PMID:15973356 TRBP recruits the Dicer complex to Ago2 for microRNA process... |
ACCEPT |
Summary: RISC-loading complex is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0001046
core promoter sequence-specific DNA binding
|
IMP
PMID:25336585 Cellular microRNAs up-regulate transcription via interaction... |
KEEP AS NON CORE |
Summary: core promoter sequence-specific DNA binding reflects a reported nuclear or regulatory context rather than the main conserved AGO2 function.
Reason: Argonaute paralogs have reported nuclear/regulatory interactions, but the dominant conserved function remains small-RNA-guided post-transcriptional repression.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005634
nucleus
|
IC
PMID:25336585 Cellular microRNAs up-regulate transcription via interaction... |
KEEP AS NON CORE |
Summary: nucleus is a plausible or reported location/context but is not the core functional site annotation for AGO2.
Reason: The core location is cytoplasmic RISC/RNP granules; nuclear, membrane, synaptic, exosomal, or other compartment annotations should remain non-core unless tied to a specific curated mechanism.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0000993
RNA polymerase II complex binding
|
IDA
PMID:25336585 Cellular microRNAs up-regulate transcription via interaction... |
KEEP AS NON CORE |
Summary: RNA polymerase II complex binding reflects a reported nuclear or regulatory context rather than the main conserved AGO2 function.
Reason: Argonaute paralogs have reported nuclear/regulatory interactions, but the dominant conserved function remains small-RNA-guided post-transcriptional repression.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:25336585 Cellular microRNAs up-regulate transcription via interaction... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0090624
endoribonuclease activity, cleaving miRNA-paired mRNA
|
IMP
PMID:22795694 Slicing-independent RISC activation requires the argonaute P... |
ACCEPT |
Summary: endoribonuclease activity, cleaving miRNA-paired mRNA is appropriate for AGO2 catalytic slicer activity.
Reason: AGO2 is the principal human slicer Argonaute and cleaves guide-complementary target RNA in RISC.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0090625
siRNA-mediated gene silencing by mRNA destabilization
|
IMP
PMID:22795694 Slicing-independent RISC activation requires the argonaute P... |
ACCEPT |
Summary: siRNA-mediated gene silencing by mRNA destabilization is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0035196
miRNA processing
|
IDA
PMID:23661684 Differential roles of human Dicer-binding proteins TRBP and ... |
ACCEPT |
Summary: miRNA processing is supported for AGO2 because AGO2 participates in RISC maturation and AGO2-specific pre-miRNA handling such as pre-miR-451 biology.
Reason: AGO2 is the catalytic Argonaute and has specific evidence for guide loading, RISC maturation, and pre-miRNA-linked processing contexts.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0031054
pre-miRNA processing
|
IDA
PMID:19966796 ATP-dependent human RISC assembly pathways. |
ACCEPT |
Summary: pre-miRNA processing is supported for AGO2 because AGO2 participates in RISC maturation and AGO2-specific pre-miRNA handling such as pre-miR-451 biology.
Reason: AGO2 is the catalytic Argonaute and has specific evidence for guide loading, RISC maturation, and pre-miRNA-linked processing contexts.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0035196
miRNA processing
|
IDA
PMID:19966796 ATP-dependent human RISC assembly pathways. |
ACCEPT |
Summary: miRNA processing is supported for AGO2 because AGO2 participates in RISC maturation and AGO2-specific pre-miRNA handling such as pre-miR-451 biology.
Reason: AGO2 is the catalytic Argonaute and has specific evidence for guide loading, RISC maturation, and pre-miRNA-linked processing contexts.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0003725
double-stranded RNA binding
|
IDA
PMID:19966796 ATP-dependent human RISC assembly pathways. |
MARK AS OVER ANNOTATED |
Summary: double-stranded RNA binding is too generic for AGO2.
Reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC rather than a broad nucleic-acid or RNA-binding label.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0003727
single-stranded RNA binding
|
IDA
PMID:19966796 ATP-dependent human RISC assembly pathways. |
MARK AS OVER ANNOTATED |
Summary: single-stranded RNA binding is too generic for AGO2.
Reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC rather than a broad nucleic-acid or RNA-binding label.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0016442
RISC complex
|
IDA
PMID:19966796 ATP-dependent human RISC assembly pathways. |
ACCEPT |
Summary: RISC complex is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0035198
miRNA binding
|
IDA
PMID:19966796 ATP-dependent human RISC assembly pathways. |
ACCEPT |
Summary: miRNA binding is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0070578
RISC-loading complex
|
IDA
PMID:19966796 ATP-dependent human RISC assembly pathways. |
ACCEPT |
Summary: RISC-loading complex is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0070922
RISC complex assembly
|
IDA
PMID:19966796 ATP-dependent human RISC assembly pathways. |
ACCEPT |
Summary: RISC complex assembly is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0090624
endoribonuclease activity, cleaving miRNA-paired mRNA
|
IDA
PMID:15260970 Human Argonaute2 mediates RNA cleavage targeted by miRNAs an... |
ACCEPT |
Summary: endoribonuclease activity, cleaving miRNA-paired mRNA is appropriate for AGO2 catalytic slicer activity.
Reason: AGO2 is the principal human slicer Argonaute and cleaves guide-complementary target RNA in RISC.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0090625
siRNA-mediated gene silencing by mRNA destabilization
|
IDA
PMID:15260970 Human Argonaute2 mediates RNA cleavage targeted by miRNAs an... |
ACCEPT |
Summary: siRNA-mediated gene silencing by mRNA destabilization is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005737
cytoplasm
|
IDA
PMID:15260970 Human Argonaute2 mediates RNA cleavage targeted by miRNAs an... |
ACCEPT |
Summary: cytoplasm is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0016442
RISC complex
|
IDA
PMID:15260970 Human Argonaute2 mediates RNA cleavage targeted by miRNAs an... |
ACCEPT |
Summary: RISC complex is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0035198
miRNA binding
|
IDA
PMID:15260970 Human Argonaute2 mediates RNA cleavage targeted by miRNAs an... |
ACCEPT |
Summary: miRNA binding is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0035279
miRNA-mediated gene silencing by mRNA destabilization
|
IDA
PMID:15260970 Human Argonaute2 mediates RNA cleavage targeted by miRNAs an... |
ACCEPT |
Summary: miRNA-mediated gene silencing by mRNA destabilization is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0070551
endoribonuclease activity, cleaving siRNA-paired mRNA
|
IDA
PMID:15260970 Human Argonaute2 mediates RNA cleavage targeted by miRNAs an... |
ACCEPT |
Summary: endoribonuclease activity, cleaving siRNA-paired mRNA is appropriate for AGO2 catalytic slicer activity.
Reason: AGO2 is the principal human slicer Argonaute and cleaves guide-complementary target RNA in RISC.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0004521
RNA endonuclease activity
|
IDA
PMID:18178619 In vitro reconstitution of the human RISC-loading complex. |
ACCEPT |
Summary: RNA endonuclease activity is appropriate for AGO2 catalytic slicer activity.
Reason: AGO2 is the principal human slicer Argonaute and cleaves guide-complementary target RNA in RISC.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0031054
pre-miRNA processing
|
IDA
PMID:18178619 In vitro reconstitution of the human RISC-loading complex. |
ACCEPT |
Summary: pre-miRNA processing is supported for AGO2 because AGO2 participates in RISC maturation and AGO2-specific pre-miRNA handling such as pre-miR-451 biology.
Reason: AGO2 is the catalytic Argonaute and has specific evidence for guide loading, RISC maturation, and pre-miRNA-linked processing contexts.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0070578
RISC-loading complex
|
IDA
PMID:18178619 In vitro reconstitution of the human RISC-loading complex. |
ACCEPT |
Summary: RISC-loading complex is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0035197
siRNA binding
|
IDA
PMID:19701182 An RNA-dependent RNA polymerase formed by TERT and the RMRP ... |
ACCEPT |
Summary: siRNA binding is a core component of AGO2 RISC loading and siRNA-guided cleavage.
Reason: AGO2 forms minimal RISC with short guide RNAs including siRNAs; siRNA guide binding is central to AGO2's RNAi/slicer activity.
Supporting Evidence:
file:human/AGO2/AGO2-uniprot.txt
The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA).
|
|
GO:0016020
membrane
|
HDA
PMID:19946888 Defining the membrane proteome of NK cells. |
KEEP AS NON CORE |
Summary: membrane is a plausible or reported location/context but is not the core functional site annotation for AGO2.
Reason: The core location is cytoplasmic RISC/RNP granules; nuclear, membrane, synaptic, exosomal, or other compartment annotations should remain non-core unless tied to a specific curated mechanism.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0003723
RNA binding
|
HDA
PMID:22658674 Insights into RNA biology from an atlas of mammalian mRNA-bi... |
MARK AS OVER ANNOTATED |
Summary: RNA binding is too generic for AGO2.
Reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC rather than a broad nucleic-acid or RNA-binding label.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0003723
RNA binding
|
HDA
PMID:22681889 The mRNA-bound proteome and its global occupancy profile on ... |
MARK AS OVER ANNOTATED |
Summary: RNA binding is too generic for AGO2.
Reason: The informative molecular function is Argonaute small-RNA/miRNA binding within RISC rather than a broad nucleic-acid or RNA-binding label.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0031047
regulatory ncRNA-mediated gene silencing
|
ISS
GO_REF:0000024 |
ACCEPT |
Summary: regulatory ncRNA-mediated gene silencing is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0060213
positive regulation of nuclear-transcribed mRNA poly(A) tail shortening
|
ISS
GO_REF:0000024 |
KEEP AS NON CORE |
Summary: positive regulation of nuclear-transcribed mRNA poly(A) tail shortening is downstream or context-specific for AGO2.
Reason: This process may be supported in a particular experiment or pathway model, but it is peripheral to the core Argonaute/RISC molecular role.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:1900153
positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
|
ISS
GO_REF:0000024 |
KEEP AS NON CORE |
Summary: positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay is downstream or context-specific for AGO2.
Reason: This process may be supported in a particular experiment or pathway model, but it is peripheral to the core Argonaute/RISC molecular role.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0000932
P-body
|
IDA
PMID:22915799 HIV-1 replication and APOBEC3 antiviral activity are not reg... |
ACCEPT |
Summary: P-body is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:16699599 Human retroviral host restriction factors APOBEC3G and APOBE... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:22915799 HIV-1 replication and APOBEC3 antiviral activity are not reg... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:16289642 Identification of novel argonaute-associated proteins. |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:20616046 LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005737
cytoplasm
|
IDA
PMID:20014101 Mouse ZAR1-like (XM_359149) colocalizes with mRNA processing... |
ACCEPT |
Summary: cytoplasm is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0005515
protein binding
|
IPI
PMID:19801630 RNA-binding motif protein 4 translocates to cytoplasmic gran... |
MARK AS OVER ANNOTATED |
Summary: Generic protein binding does not capture the specific Argonaute/RISC function.
Reason: The cited interactions are best interpreted as RISC/cofactor context for small-RNA-guided silencing, not as a standalone core function.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0035278
miRNA-mediated gene silencing by inhibition of translation
|
IDA
PMID:19801630 RNA-binding motif protein 4 translocates to cytoplasmic gran... |
ACCEPT |
Summary: miRNA-mediated gene silencing by inhibition of translation is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0045947
negative regulation of translational initiation
|
IDA
PMID:19801630 RNA-binding motif protein 4 translocates to cytoplasmic gran... |
KEEP AS NON CORE |
Summary: negative regulation of translational initiation is downstream or context-specific for AGO2.
Reason: This process may be supported in a particular experiment or pathway model, but it is peripheral to the core Argonaute/RISC molecular role.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0016442
RISC complex
|
IDA
PMID:17932509 Proteomic and functional analysis of Argonaute-containing mR... |
ACCEPT |
Summary: RISC complex is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0000932
P-body
|
IDA
PMID:20616046 LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for... |
ACCEPT |
Summary: P-body is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0016442
RISC complex
|
IDA
PMID:20616046 LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for... |
ACCEPT |
Summary: RISC complex is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0070551
endoribonuclease activity, cleaving siRNA-paired mRNA
|
IDA
PMID:17524464 An mRNA m7G cap binding-like motif within human Ago2 repress... |
ACCEPT |
Summary: endoribonuclease activity, cleaving siRNA-paired mRNA is appropriate for AGO2 catalytic slicer activity.
Reason: AGO2 is the principal human slicer Argonaute and cleaves guide-complementary target RNA in RISC.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0000340
RNA 7-methylguanosine cap binding
|
IDA
PMID:17524464 An mRNA m7G cap binding-like motif within human Ago2 repress... |
KEEP AS NON CORE |
Summary: RNA 7-methylguanosine cap binding is experimentally supported for AGO2-mediated translational repression, but is not the main AGO2 slicer/RISC function.
Reason: This is a specific cap-binding activity supported by PMID:17524464 and UniProt, best retained as a non-core translational-repression mechanism.
Supporting Evidence:
PMID:17524464
We identify conserved aromatic residues within the MC motif of human Ago2 that are required for binding to the m(7)G cap and for translational repression but do not affect the assembly of Ago2 with miRNA or its catalytic activity.
file:human/AGO2/AGO2-uniprot.txt
May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E.
|
|
GO:0035197
siRNA binding
|
IDA
PMID:15973356 TRBP recruits the Dicer complex to Ago2 for microRNA process... |
ACCEPT |
Summary: siRNA binding is a core component of AGO2 RISC loading and siRNA-guided cleavage.
Reason: AGO2 forms minimal RISC with short guide RNAs including siRNAs; siRNA guide binding is central to AGO2's RNAi/slicer activity.
Supporting Evidence:
file:human/AGO2/AGO2-uniprot.txt
The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA).
|
|
GO:0016442
RISC complex
|
IDA
PMID:17671087 Let-7 microRNA-mediated mRNA deadenylation and translational... |
ACCEPT |
Summary: RISC complex is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0031054
pre-miRNA processing
|
IDA
PMID:17671087 Let-7 microRNA-mediated mRNA deadenylation and translational... |
ACCEPT |
Summary: pre-miRNA processing is supported for AGO2 because AGO2 participates in RISC maturation and AGO2-specific pre-miRNA handling such as pre-miR-451 biology.
Reason: AGO2 is the catalytic Argonaute and has specific evidence for guide loading, RISC maturation, and pre-miRNA-linked processing contexts.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0035197
siRNA binding
|
IDA
PMID:17495927 The human RNA kinase hClp1 is active on 3' transfer RNA exon... |
ACCEPT |
Summary: siRNA binding is a core component of AGO2 RISC loading and siRNA-guided cleavage.
Reason: AGO2 forms minimal RISC with short guide RNAs including siRNAs; siRNA guide binding is central to AGO2's RNAi/slicer activity.
Supporting Evidence:
file:human/AGO2/AGO2-uniprot.txt
The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA).
|
|
GO:0035278
miRNA-mediated gene silencing by inhibition of translation
|
IDA
PMID:17671087 Let-7 microRNA-mediated mRNA deadenylation and translational... |
ACCEPT |
Summary: miRNA-mediated gene silencing by inhibition of translation is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0035278
miRNA-mediated gene silencing by inhibition of translation
|
IMP
PMID:18771919 Importance of translation and nonnucleolytic ago proteins fo... |
ACCEPT |
Summary: miRNA-mediated gene silencing by inhibition of translation is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0035279
miRNA-mediated gene silencing by mRNA destabilization
|
IDA
PMID:17524464 An mRNA m7G cap binding-like motif within human Ago2 repress... |
ACCEPT |
Summary: miRNA-mediated gene silencing by mRNA destabilization is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0035279
miRNA-mediated gene silencing by mRNA destabilization
|
IMP
PMID:18771919 Importance of translation and nonnucleolytic ago proteins fo... |
ACCEPT |
Summary: miRNA-mediated gene silencing by mRNA destabilization is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
Reason: The falcon report supports AGO2 function in miRISC-mediated small-RNA-guided repression and related RISC complexes or cytoplasmic RNP compartments.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0045947
negative regulation of translational initiation
|
IDA
PMID:17524464 An mRNA m7G cap binding-like motif within human Ago2 repress... |
KEEP AS NON CORE |
Summary: negative regulation of translational initiation is downstream or context-specific for AGO2.
Reason: This process may be supported in a particular experiment or pathway model, but it is peripheral to the core Argonaute/RISC molecular role.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0003743
translation initiation factor activity
|
NAS
PMID:10534406 Human eukaryotic initiation factor EIF2C1 gene: cDNA sequenc... |
REMOVE |
Summary: translation initiation factor activity is a legacy implication of the EIF2C name and is not supported as an Argonaute activity.
Reason: Human Argonautes are RISC effector proteins, not translation initiation factors in the canonical eIF sense.
Supporting Evidence:
file:human/AGO2/AGO2-deep-research-falcon.md
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11.
|
|
GO:0006412
translation
|
NAS
PMID:10534406 Human eukaryotic initiation factor EIF2C1 gene: cDNA sequenc... |
REMOVE |
Summary: translation is a legacy EIF2C-name annotation and is not supported as a direct AGO2 biological-process annotation by this reference.
Reason: PMID:10534406 supports cloning and expression of the EIF2C1/Argonaute-family gene, not AGO2 participation in the broad biological process of translation. Direct AGO2 translation-related annotations should be limited to supported translational-repression terms.
Supporting Evidence:
PMID:10534406
We report the cloning and characterization of the human eukaryotic protein translation initiation factor EIF2C1 gene.
|
Q: Which endogenous human tissues or stress states require AGO2-specific RISC activity rather than redundant activity from other AGO paralogs?
Q: Which protein cofactors determine guide loading, localization, and non-core nuclear or granule-associated functions of AGO2?
Experiment: Endogenous tagging of AGO2 followed by small-RNA CLIP, target RNA profiling, and paralog-specific rescue in AGO-depleted human cells.
Hypothesis: AGO2 has paralog-specific guide, target, and compartment preferences within human RISC biology.
Type: genome editing/RNA-protein interaction profiling
Experiment: Biochemical reconstitution of AGO2-RISC with representative miRNA and siRNA guides to compare target repression, target cleavage, and TNRC6 recruitment.
Hypothesis: AGO2 activity is best explained by guide-loaded RISC function rather than generic RNA or protein binding.
Type: biochemical reconstitution
The research report should be a detailed narrative explaining the function, biological processes, and localization of the gene product. Citations should be given for all claims.
You should prioritize authoritative reviews and primary scientific literature when conducting research. You can supplement
this with annotations you find in gene/protein databases, but these can be outdated or inaccurate.
We are specifically interested in the primary function of the gene - for enzymes, what reaction is catalyzed, and what is the substrate specificity? For transporters, what is the substrate? For structural proteins or adapters, what is the broader structural role? For signaling molecules, what is the role in the pathway.
We are interested in where in or outside the cell the gene product carries out its function.
We are also interested in the signaling or biochemical pathways in which the gene functions. We are less interested in broad pleiotropic effects, except where these elucidate the precise role.
Include evidence where possible. We are interested in both experimental evidence as well as inference from structure, evolution, or bioinformatic analysis. Precise studies should be prioritized over high-throughput, where available.
The target protein is human Argonaute-2 (AGO2; synonym EIF2C2), a member of the Argonaute (AGO) family and central effector of the RNA-induced silencing complex (RISC). Recent mechanistic reviews describe human AGO2 (HsAGO2) as a “long” Argonaute with the canonical N, PAZ, MID, and PIWI domains and PIWI RNase H-like endonuclease activity (slicer function), matching the UniProt description for Q9UKV8. (nakanishi2024whenargonautetakes pages 1-3, shah2024uncoveringtheregulation pages 35-39)
Argonaute (AGO) proteins bind short guide RNAs and form RISCs that recognize complementary RNA targets. Long eukaryotic AGOs adopt a bilobed architecture (N–PAZ lobe and MID–PIWI lobe) that positions the guide RNA for target recognition and repression/cleavage. (nakanishi2024whenargonautetakes pages 1-3, nakanishi2024whenargonautetakes media bddc4fe3)
Human Argonaute domain roles are described as follows: the PAZ domain anchors the guide RNA 3′ end (classically a 2-nt 3′ overhang); the MID domain binds the guide 5′ end (often a 5′ phosphate); and the PIWI domain is RNase H-like and houses the catalytic center and effector-binding surfaces. The N-terminal domain supports duplex unwinding/loading. (shah2024uncoveringtheregulation pages 35-39)
In animals, miRNA targeting typically involves partial complementarity dominated by seed pairing (guide positions g2–g8), and repression commonly proceeds through translational repression followed by mRNA destabilization rather than cleavage. In contrast, near-perfect complementarity can enable AGO-mediated endonucleolytic cleavage (“slicing”). (shah2024uncoveringtheregulation pages 35-39, nakanishi2024whenargonautetakes pages 1-3)
A recent Journal of Biological Chemistry review provides a schematic showing long-AGO domain architecture and a guide–target pairing diagram with the canonical cleavage site between target t10 and t11. (nakanishi2024whenargonautetakes media bddc4fe3)
Human AGO2 is an RNA-guided endoribonuclease that hydrolyzes the phosphodiester backbone of a target RNA strand when it is base-paired to a guide RNA within RISC. Structural/mechanistic descriptions support a two–metal-ion mechanism resembling RNase H chemistry, in which divalent cations coordinate the scissile phosphate and activate a water nucleophile for hydrolysis. (nakanishi2024whenargonautetakes pages 9-11, mikutis2024technologiesfortargeted pages 5-7)
Substrate: AGO2 cleaves RNA targets that form an extensive guide:target duplex (not merely seed pairing). The canonical scissile bond is between target nucleotides t10 and t11 (relative to the guide). (nakanishi2024whenargonautetakes pages 4-7, nakanishi2024whenargonautetakes pages 1-3)
A recent mechanistic synthesis notes that guide-target recognition is organized into functional segments (seed, central, 3′ supplemental, tail) and that slicing-competent configurations require pairing that positions the target phosphate at t10–t11 in the catalytic center. (nakanishi2024whenargonautetakes pages 1-3, nakanishi2024whenargonautetakes pages 4-7)
AGO2 catalytic competence is linked to a PIWI-domain DEDH tetrad (described as essential for slicing activity), and divalent metal ions are required. One recent review reports that Mg2+ (and Mn2+, Ni2+, Co2+) activates human AGO2 better than Ca2+ for slicing. (shah2024uncoveringtheregulation pages 35-39, nakanishi2024whenargonautetakes pages 4-7)
Structural discussion further places metal ions beneath the scissile phosphate at t10/t11 and details metal coordination in HsAGO2 structures, consistent with a two–metal-ion catalytic model. (nakanishi2024whenargonautetakes pages 7-9, nakanishi2024whenargonautetakes pages 9-11)
Human AGO2 is emphasized as a slicer capable of cleaving complementary RNAs; examples discussed include cleavage of complementary mRNAs (e.g., HOXB8 targeted by miR-196 in a canonical example) and certain pre-miRNAs (e.g., pre-miR-451) loaded into AGO2. (nakanishi2024whenargonautetakes pages 9-11)
In animal miRNA repression, AGO proteins recruit GW182/TNRC6 to connect target binding with mRNA decay machinery. GW182 acts as a scaffold that promotes loss of PABP from the poly(A) tail and recruitment of deadenylases (CCR4-NOT and PAN2-PAN3), followed by recruitment of decapping factors (DCP2, with activators such as DCP1, DDX6/RCK, and 4E-T) and exonucleolytic decay (e.g., XRN1). (shah2024uncoveringtheregulation pages 39-44)
P-bodies are cytoplasmic RNP condensates enriched for decapping/decay factors and mRNAs in repressed/decay-linked states. A 2023 EMBO Journal study emphasizes that miRNA-targeted mRNAs can be stabilized in a translationally repressed state when decapping is inhibited and that P-body dynamics can modulate the balance between repression and decapping/decay. (brothers2023theedc4‐xrn1interaction pages 1-2)
Consistent with this, recent synthesis work links Argonaute-associated repression to cytoplasmic condensates and recruitment of CCR4-NOT deadenylation machinery. (blagojevic2024heatstresspromotes pages 13-15)
A 2024 review focused on nuclear miRNAs reports detection of a smaller nuclear AGO2–miRNA RISC (~158 kDa) compared with cytoplasmic RISC (~3 MDa). It further reports that nuclear RISC composition includes GW182/TNRC6, while AGO2 interactions with DICER and TRBP are described as restricted to the cytoplasm. Nucleocytoplasmic transport factors discussed include Importin 8 (import) and Exportin 1/CRM1 (export). (billi2024nuclearmirnasgene pages 2-4, billi2024nuclearmirnasgene pages 1-2)
These findings support a model in which AGO2-containing complexes can also operate in the nucleus in selected contexts, though canonical target repression and slicer activity are predominantly discussed in the cytoplasmic setting. (billi2024nuclearmirnasgene pages 1-2, shah2024uncoveringtheregulation pages 39-44)
A 2024 synthesis of Argonaute phosphorylation describes a conserved PIWI-domain phosphorylation cluster (reported as S824, S828, T830, S831, S834 in AGO2) and proposes a kinase/phosphatase cycle that modulates AGO2’s interaction with mRNA targets and miRNA-mediated silencing dynamics, supporting a “recycling” concept for RISC on targets. (shah2024uncoveringtheregulation pages 48-52)
A major 2024 development emphasized in two reviews is target-directed miRNA degradation (TDMD). In TDMD, trigger RNAs with unusually extensive complementarity bind the AGO–miRNA complex and recruit the ZSWIM8 ubiquitin ligase complex, leading to AGO ubiquitination and proteasomal degradation of AGO, after which the miRNA becomes vulnerable to nucleases. (hiers2024target‐directedmicrornadegradation pages 1-3, buhagiar2024tokilla pages 3-4)
Quantitative perspective from a 2024 Nucleic Acids Research review: miRNAs often have half-lives >12–24 h, ~5% have half-lives <12 h, ZSWIM8 regulates >100 miRNAs across bilaterians, and Cyrano can eliminate >98% of miR-7 in some mouse tissues. (buhagiar2024tokilla pages 1-3)
Clinical siRNA drugs depend on cellular loading of the guide strand into AGO2-containing RISC, followed by binding and cleavage of complementary target RNAs. (fazoli2024rnainterferencetherapy pages 1-3, mikutis2024technologiesfortargeted pages 5-7)
A 2024 Chemical Reviews article lists six FDA/EMA-approved siRNA therapeutics (2018–2023)—patisiran, givosiran, lumasiran, inclisiran, vutrisiran, and nedosiran—and highlights that, in that accounting, all six target liver-expressed mRNAs, underscoring the practical importance of liver-targeting delivery approaches. (mikutis2024technologiesfortargeted pages 5-7)
Two enabling delivery strategies highlighted in recent translational reviews are:
* Lipid nanoparticles (LNPs): delivery vehicles that promote intracellular uptake and endosomal escape.
* GalNAc conjugation: hepatocyte-targeting strategy leveraging receptor-mediated uptake in the liver.
These approaches are described as central to achieving sufficient cytoplasmic delivery for productive RISC formation and target RNA cleavage. (mikutis2024technologiesfortargeted pages 5-7, fazoli2024rnainterferencetherapy pages 1-3)
Therapeutic siRNAs commonly use ribose modifications (e.g., 2′-F, 2′-OMe) to improve nuclease resistance while preserving RISC loading and activity. (mikutis2024technologiesfortargeted pages 5-7)
Human AGO2 cleaves guide-complementary target RNA at the phosphodiester bond between target positions t10 and t11. (nakanishi2024whenargonautetakes pages 1-3)
Mature miRNAs are usually long-lived, with typical half-lives greater than 12–24 hours; about ~5% are shorter-lived (<12 hours), and some miRNA half-lives can extend to several days. (buhagiar2024tokilla pages 1-3, hiers2024target‐directedmicrornadegradation pages 3-5)
ZSWIM8-dependent TDMD regulates more than 100 miRNAs across bilaterian animals, and the lncRNA Cyrano can eliminate more than 98% of miR-7 in some mouse tissues. (buhagiar2024tokilla pages 1-3, buhagiar2024tokilla pages 3-4)
A 2024 Chemical Reviews article lists six FDA/EMA-approved siRNA therapeutics approved from 2018 to 2023: patisiran, givosiran, lumasiran, inclisiran, vutrisiran, and nedosiran. (mikutis2024technologiesfortargeted pages 5-7)
Canonical cytoplasmic RISC has been reported at approximately ~3 MDa, whereas a smaller nuclear AGO2–miRNA RISC has been detected at approximately ~158 kDa. (billi2024nuclearmirnasgene pages 2-4, billi2024nuclearmirnasgene pages 1-2)
Blockquote: This blockquote collects the main quantitative facts relevant to human AGO2 biology and translation, spanning catalytic position, miRNA stability, TDMD scale, approved siRNA drugs, and RISC complex sizes. It is useful as a compact evidence-backed reference for the report.
Recent expert reviews converge on a mechanistic “division of labor” for AGO2: (i) catalytic slicing as a precise mechanism for guide-complementary targets and engineered/therapeutic siRNAs, and (ii) cleavage-independent repression as the dominant mode for endogenous animal miRNA regulation via GW182/TNRC6-mediated recruitment of deadenylation/decapping and mRNA decay pathways. (shah2024uncoveringtheregulation pages 35-39, shah2024uncoveringtheregulation pages 39-44)
In parallel, 2024 TDMD-focused reviews elevate regulated miRNA turnover (via ZSWIM8 and conformational recognition of trigger-bound AGO–miRNA complexes) as a key dimension of small-RNA biology that complements traditional emphases on biogenesis and target repression. (buhagiar2024tokilla pages 1-3, buhagiar2024tokilla pages 3-4)
| Aspect | Summary |
|---|---|
| Identity/synonyms | Human AGO2 corresponds to Argonaute-2 / hAgo2 / EIF2C2, the catalytic Argonaute family member in mammalian RISC; AGO proteins are core effectors of miRNA/siRNA silencing. (nakanishi2024whenargonautetakes pages 1-3, shah2024uncoveringtheregulation pages 30-35) |
| Core molecular function | Core RISC component that binds small-RNA guides and silences complementary RNAs. AGO2 supports both cleavage-independent repression of most animal miRNA targets and endonucleolytic slicing of highly complementary targets/siRNA targets. (nakanishi2024whenargonautetakes pages 1-3, shah2024uncoveringtheregulation pages 35-39, zapletalUnknownyearstructuralbasisof pages 18-20) |
| Catalytic mechanism & site | The PIWI domain has an RNase H-like active site with catalytic DEDH tetrad; the glutamate is essential for slicing. Human AGO2 cleaves target RNA using divalent cations, with Mg2+/Mn2+ supporting activity better than Ca2+. (shah2024uncoveringtheregulation pages 35-39, nakanishi2024whenargonautetakes pages 4-7) |
| Guide/target requirements & cleavage position | AGO2 binds a small-RNA guide whose 5′ end is anchored in MID and 3′ end in PAZ. Target recognition begins with the seed (guide g2-g8); near-perfect pairing can enable slicing, with cleavage occurring between target positions t10 and t11. (nakanishi2024whenargonautetakes pages 1-3, nakanishi2024whenargonautetakes pages 3-4, nakanishi2024whenargonautetakes media bddc4fe3) |
| Domain architecture (N/PAZ/MID/PIWI) | Long AGO proteins have a bilobed architecture with N, PAZ, MID, and PIWI domains plus linkers. N helps duplex unwinding/loading, PAZ binds the guide 3′ overhang, MID recognizes the 5′ phosphate, and PIWI mediates catalysis and effector interactions. (nakanishi2024whenargonautetakes pages 1-3, shah2024uncoveringtheregulation pages 35-39, nakanishi2024whenargonautetakes media bddc4fe3) |
| Cofactors/partners (GW182/TNRC6, CCR4-NOT, PAN2-PAN3, Dicer/TRBP) | In miRNA repression, AGO2 engages GW182/TNRC6, which recruits CCR4-NOT and PAN2-PAN3 to drive deadenylation, decapping, and decay. During biogenesis/loading, Dicer and TRBP help generate/load small RNAs into AGO2-containing RISC. (shah2024uncoveringtheregulation pages 35-39, hiers2024target‐directedmicrornadegradation pages 3-5) |
| Regulation (phosphorylation cluster, TDMD/ZSWIM8) | A conserved PIWI-domain phosphorylation cluster (S824, S828, T830, S831, S834) modulates target binding/release and AGO recycling. In TDMD, highly complementary trigger RNAs recruit ZSWIM8 E3 ligase, causing AGO ubiquitination/proteolysis and subsequent miRNA decay. (shah2024uncoveringtheregulation pages 48-52, hiers2024target‐directedmicrornadegradation pages 1-3, buhagiar2024tokilla pages 3-4) |
| Localization/condensates | AGO2 functions mainly in the cytoplasm within RISC and is linked to P-bodies/stress-granule-associated mRNP regulation; recent reviews also discuss mature miRNA/AGO activities in the nucleus in some contexts, though canonical silencing is cytoplasmic. (shah2024uncoveringtheregulation pages 39-44, nakanishi2024whenargonautetakes media bddc4fe3) |
| Applications (approved siRNA drugs, delivery strategies) | Clinical siRNA drugs rely on AGO2 loading into RISC for target RNA cleavage. Real-world implementations include approved liver-directed siRNA medicines, enabled mainly by lipid nanoparticles (LNPs) or GalNAc conjugation for hepatocyte delivery. (mikutis2024technologiesfortargeted pages 5-7, fazoli2024rnainterferencetherapy pages 1-3) |
| Key quantitative data | Typical miRNA half-lives are often >12-24 h, and can exceed several days; ~5% are shorter-lived (<12 h). Reviews report 5-6 approved siRNA therapeutics by 2024 depending on counting/approval timing, all liver-targeted in one 2024 review; >100 miRNAs are regulated by ZSWIM8 across bilaterians, and Cyrano can eliminate >98% of miR-7 in some mouse tissues. (buhagiar2024tokilla pages 1-3, hiers2024target‐directedmicrornadegradation pages 3-5, mikutis2024technologiesfortargeted pages 5-7, motamedi2024principleapplicationand pages 1-2) |
Table: This table summarizes the main functional annotation points for human AGO2 (UniProt Q9UKV8), including mechanism, domains, partners, regulation, localization, and translational relevance. It provides a compact evidence-linked reference for building the full research report.
References
(nakanishi2024whenargonautetakes pages 1-3): Kotaro Nakanishi. When argonaute takes out the ribonuclease sword. Journal of Biological Chemistry, 300:105499, Jan 2024. URL: https://doi.org/10.1016/j.jbc.2023.105499, doi:10.1016/j.jbc.2023.105499. This article has 25 citations and is from a domain leading peer-reviewed journal.
(shah2024uncoveringtheregulation pages 35-39): VN Shah. Uncovering the regulation and implication of argonaute phosphorylation in the microrna pathway. Unknown journal, 2024.
(nakanishi2024whenargonautetakes media bddc4fe3): Kotaro Nakanishi. When argonaute takes out the ribonuclease sword. Journal of Biological Chemistry, 300:105499, Jan 2024. URL: https://doi.org/10.1016/j.jbc.2023.105499, doi:10.1016/j.jbc.2023.105499. This article has 25 citations and is from a domain leading peer-reviewed journal.
(nakanishi2024whenargonautetakes pages 9-11): Kotaro Nakanishi. When argonaute takes out the ribonuclease sword. Journal of Biological Chemistry, 300:105499, Jan 2024. URL: https://doi.org/10.1016/j.jbc.2023.105499, doi:10.1016/j.jbc.2023.105499. This article has 25 citations and is from a domain leading peer-reviewed journal.
(mikutis2024technologiesfortargeted pages 5-7): Sigitas Mikutis and Gonçalo J. L. Bernardes. Technologies for targeted rna degradation and induced rna decay. Chemical Reviews, 124:13301-13330, Nov 2024. URL: https://doi.org/10.1021/acs.chemrev.4c00472, doi:10.1021/acs.chemrev.4c00472. This article has 27 citations and is from a highest quality peer-reviewed journal.
(nakanishi2024whenargonautetakes pages 4-7): Kotaro Nakanishi. When argonaute takes out the ribonuclease sword. Journal of Biological Chemistry, 300:105499, Jan 2024. URL: https://doi.org/10.1016/j.jbc.2023.105499, doi:10.1016/j.jbc.2023.105499. This article has 25 citations and is from a domain leading peer-reviewed journal.
(nakanishi2024whenargonautetakes pages 7-9): Kotaro Nakanishi. When argonaute takes out the ribonuclease sword. Journal of Biological Chemistry, 300:105499, Jan 2024. URL: https://doi.org/10.1016/j.jbc.2023.105499, doi:10.1016/j.jbc.2023.105499. This article has 25 citations and is from a domain leading peer-reviewed journal.
(shah2024uncoveringtheregulation pages 39-44): VN Shah. Uncovering the regulation and implication of argonaute phosphorylation in the microrna pathway. Unknown journal, 2024.
(brothers2023theedc4‐xrn1interaction pages 1-2): William R Brothers, Farah Ali, Sam Kajjo, and Marc R Fabian. The edc4‐xrn1 interaction controls p‐body dynamics to link mrna decapping with decay. The EMBO Journal, Aug 2023. URL: https://doi.org/10.15252/embj.2023113933, doi:10.15252/embj.2023113933. This article has 43 citations.
(blagojevic2024heatstresspromotes pages 13-15): Aleksandar Blagojevic, Patricia Baldrich, Marlene Schiaffini, Esther Lechner, Nicolas Baumberger, Philippe Hammann, Taline Elmayan, Damien Garcia, Hervé Vaucheret, Blake C. Meyers, and Pascal Genschik. Heat stress promotes arabidopsis ago1 phase separation and association with stress granule components. iScience, 27:109151, Mar 2024. URL: https://doi.org/10.1016/j.isci.2024.109151, doi:10.1016/j.isci.2024.109151. This article has 26 citations and is from a peer-reviewed journal.
(billi2024nuclearmirnasgene pages 2-4): Monia Billi, Elisabetta De Marinis, Martina Gentile, Clara Nervi, and Francesco Grignani. Nuclear mirnas: gene regulation activities. International Journal of Molecular Sciences, May 2024. URL: https://doi.org/10.3390/ijms25116066, doi:10.3390/ijms25116066. This article has 62 citations.
(billi2024nuclearmirnasgene pages 1-2): Monia Billi, Elisabetta De Marinis, Martina Gentile, Clara Nervi, and Francesco Grignani. Nuclear mirnas: gene regulation activities. International Journal of Molecular Sciences, May 2024. URL: https://doi.org/10.3390/ijms25116066, doi:10.3390/ijms25116066. This article has 62 citations.
(shah2024uncoveringtheregulation pages 48-52): VN Shah. Uncovering the regulation and implication of argonaute phosphorylation in the microrna pathway. Unknown journal, 2024.
(hiers2024target‐directedmicrornadegradation pages 1-3): Nicholas M. Hiers, Tianqi Li, Conner M. Traugot, and Mingyi Xie. Target‐directed microrna degradation: mechanisms, significance, and functional implications. Wiley Interdisciplinary Reviews: RNA, Mar 2024. URL: https://doi.org/10.1002/wrna.1832, doi:10.1002/wrna.1832. This article has 36 citations.
(buhagiar2024tokilla pages 3-4): Amber F. Buhagiar and Benjamin Kleaveland. To kill a microrna: emerging concepts in target-directed microrna degradation. Nucleic Acids Research, 52:1558-1574, Jan 2024. URL: https://doi.org/10.1093/nar/gkae003, doi:10.1093/nar/gkae003. This article has 56 citations and is from a highest quality peer-reviewed journal.
(buhagiar2024tokilla pages 1-3): Amber F. Buhagiar and Benjamin Kleaveland. To kill a microrna: emerging concepts in target-directed microrna degradation. Nucleic Acids Research, 52:1558-1574, Jan 2024. URL: https://doi.org/10.1093/nar/gkae003, doi:10.1093/nar/gkae003. This article has 56 citations and is from a highest quality peer-reviewed journal.
(fazoli2024rnainterferencetherapy pages 1-3): Renata TJ Fazoli, Luciano F Drager, Roberto Kalil-Filho, and Giuliano Generoso. Rna interference therapy in cardiology: will new targets improve therapeutic goals? Drugs in Context, 13:1-11, Aug 2024. URL: https://doi.org/10.7573/dic.2024-3-1, doi:10.7573/dic.2024-3-1. This article has 7 citations.
(hiers2024target‐directedmicrornadegradation pages 3-5): Nicholas M. Hiers, Tianqi Li, Conner M. Traugot, and Mingyi Xie. Target‐directed microrna degradation: mechanisms, significance, and functional implications. Wiley Interdisciplinary Reviews: RNA, Mar 2024. URL: https://doi.org/10.1002/wrna.1832, doi:10.1002/wrna.1832. This article has 36 citations.
(shah2024uncoveringtheregulation pages 30-35): VN Shah. Uncovering the regulation and implication of argonaute phosphorylation in the microrna pathway. Unknown journal, 2024.
(zapletalUnknownyearstructuralbasisof pages 18-20): D ZAPLETAL. Structural basis of mirna processing in mammals. Unknown journal, Unknown year.
(nakanishi2024whenargonautetakes pages 3-4): Kotaro Nakanishi. When argonaute takes out the ribonuclease sword. Journal of Biological Chemistry, 300:105499, Jan 2024. URL: https://doi.org/10.1016/j.jbc.2023.105499, doi:10.1016/j.jbc.2023.105499. This article has 25 citations and is from a domain leading peer-reviewed journal.
(motamedi2024principleapplicationand pages 1-2): Hamid Motamedi, Marzie Mahdizade Ari, Amirhoushang Alvandi, and Ramin Abiri. Principle, application and challenges of development sirna-based therapeutics against bacterial and viral infections: a comprehensive review. Frontiers in Microbiology, Jun 2024. URL: https://doi.org/10.3389/fmicb.2024.1393646, doi:10.3389/fmicb.2024.1393646. This article has 43 citations and is from a peer-reviewed journal.
id: Q9UKV8
gene_symbol: AGO2
product_type: PROTEIN
status: COMPLETE
taxon:
id: NCBITaxon:9606
label: Homo sapiens
description: 'AGO2 encodes human Argonaute-2, the principal catalytic Argonaute in
mammalian RNA silencing. AGO2 binds miRNA or siRNA guides in RISC and can repress
targets non-catalytically through miRISC effector recruitment or cleave highly complementary
RNA targets as an RNA-guided endoribonuclease.'
alternative_products:
- name: '1'
id: Q9UKV8-1
- name: '2'
id: Q9UKV8-2
sequence_note: VSP_037001
existing_annotations:
- term:
id: GO:0005634
label: nucleus
evidence_type: IBA
original_reference_id: GO_REF:0000033
review:
summary: nucleus is a plausible or reported location/context but is not the
core functional site annotation for AGO2.
action: KEEP_AS_NON_CORE
reason: The core location is cytoplasmic RISC/RNP granules; nuclear,
membrane, synaptic, exosomal, or other compartment annotations should
remain non-core unless tied to a specific curated mechanism.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005737
label: cytoplasm
evidence_type: IBA
original_reference_id: GO_REF:0000033
review:
summary: cytoplasm is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0004521
label: RNA endonuclease activity
evidence_type: IBA
original_reference_id: GO_REF:0000033
review:
summary: RNA endonuclease activity is appropriate for AGO2 catalytic slicer
activity.
action: ACCEPT
reason: AGO2 is the principal human slicer Argonaute and cleaves
guide-complementary target RNA in RISC.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0035194
label: regulatory ncRNA-mediated post-transcriptional gene silencing
evidence_type: IBA
original_reference_id: GO_REF:0000033
review:
summary: regulatory ncRNA-mediated post-transcriptional gene silencing is
consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0016442
label: RISC complex
evidence_type: IBA
original_reference_id: GO_REF:0000033
review:
summary: RISC complex is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0036464
label: cytoplasmic ribonucleoprotein granule
evidence_type: IBA
original_reference_id: GO_REF:0000033
review:
summary: cytoplasmic ribonucleoprotein granule is consistent with AGO2 as a
small-RNA-loaded Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0031054
label: pre-miRNA processing
evidence_type: IBA
original_reference_id: GO_REF:0000033
review:
summary: pre-miRNA processing is supported for AGO2 because AGO2
participates in RISC maturation and AGO2-specific pre-miRNA handling such
as pre-miR-451 biology.
action: ACCEPT
reason: AGO2 is the catalytic Argonaute and has specific evidence for guide
loading, RISC maturation, and pre-miRNA-linked processing contexts.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0035198
label: miRNA binding
evidence_type: IBA
original_reference_id: GO_REF:0000033
review:
summary: miRNA binding is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0003727
label: single-stranded RNA binding
evidence_type: IBA
original_reference_id: GO_REF:0000033
review:
summary: single-stranded RNA binding is too generic for AGO2.
action: MARK_AS_OVER_ANNOTATED
reason: The informative molecular function is Argonaute small-RNA/miRNA
binding within RISC rather than a broad nucleic-acid or RNA-binding label.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0000340
label: RNA 7-methylguanosine cap binding
evidence_type: IEA
original_reference_id: GO_REF:0000104
review:
summary: RNA 7-methylguanosine cap binding is experimentally supported for
AGO2-mediated translational repression, but is not the main AGO2
slicer/RISC function.
action: KEEP_AS_NON_CORE
reason: This is a specific cap-binding activity supported by PMID:17524464
and UniProt, best retained as a non-core translational-repression
mechanism.
supported_by: &id001
- reference_id: PMID:17524464
supporting_text: We identify conserved aromatic residues within the MC
motif of human Ago2 that are required for binding to the m(7)G cap and
for translational repression but do not affect the assembly of Ago2 with
miRNA or its catalytic activity.
- reference_id: file:human/AGO2/AGO2-uniprot.txt
supporting_text: May inhibit translation initiation by binding to the
7-methylguanosine cap, thereby preventing the recruitment of the
translation initiation factor eIF4-E.
- term:
id: GO:0000932
label: P-body
evidence_type: IEA
original_reference_id: GO_REF:0000120
review:
summary: P-body is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC
effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0003676
label: nucleic acid binding
evidence_type: IEA
original_reference_id: GO_REF:0000002
review:
summary: nucleic acid binding is too generic for AGO2.
action: MARK_AS_OVER_ANNOTATED
reason: The informative molecular function is Argonaute small-RNA/miRNA
binding within RISC rather than a broad nucleic-acid or RNA-binding label.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0003723
label: RNA binding
evidence_type: IEA
original_reference_id: GO_REF:0000120
review:
summary: RNA binding is too generic for AGO2.
action: MARK_AS_OVER_ANNOTATED
reason: The informative molecular function is Argonaute small-RNA/miRNA
binding within RISC rather than a broad nucleic-acid or RNA-binding label.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0003725
label: double-stranded RNA binding
evidence_type: IEA
original_reference_id: GO_REF:0000117
review:
summary: double-stranded RNA binding is too generic for AGO2.
action: MARK_AS_OVER_ANNOTATED
reason: The informative molecular function is Argonaute small-RNA/miRNA
binding within RISC rather than a broad nucleic-acid or RNA-binding label.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0003727
label: single-stranded RNA binding
evidence_type: IEA
original_reference_id: GO_REF:0000117
review:
summary: single-stranded RNA binding is too generic for AGO2.
action: MARK_AS_OVER_ANNOTATED
reason: The informative molecular function is Argonaute small-RNA/miRNA
binding within RISC rather than a broad nucleic-acid or RNA-binding label.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005634
label: nucleus
evidence_type: IEA
original_reference_id: GO_REF:0000120
review:
summary: nucleus is a plausible or reported location/context but is not the
core functional site annotation for AGO2.
action: KEEP_AS_NON_CORE
reason: The core location is cytoplasmic RISC/RNP granules; nuclear,
membrane, synaptic, exosomal, or other compartment annotations should
remain non-core unless tied to a specific curated mechanism.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0006355
label: regulation of DNA-templated transcription
evidence_type: IEA
original_reference_id: GO_REF:0000002
review:
summary: regulation of DNA-templated transcription reflects a reported
nuclear or regulatory context rather than the main conserved AGO2
function.
action: KEEP_AS_NON_CORE
reason: Argonaute paralogs have reported nuclear/regulatory interactions,
but the dominant conserved function remains small-RNA-guided
post-transcriptional repression.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0006413
label: translational initiation
evidence_type: IEA
original_reference_id: GO_REF:0000108
review:
summary: translational initiation is a legacy implication of the EIF2C name
and is not supported as an Argonaute activity.
action: REMOVE
reason: Human Argonautes are RISC effector proteins, not translation
initiation factors in the canonical eIF sense.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0016442
label: RISC complex
evidence_type: IEA
original_reference_id: GO_REF:0000120
review:
summary: RISC complex is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0016891
label: RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic
mechanism
evidence_type: IEA
original_reference_id: GO_REF:0000104
review:
summary: RNA endonuclease activity producing 5'-phosphomonoesters,
hydrolytic mechanism is appropriate for AGO2 catalytic slicer activity.
action: ACCEPT
reason: AGO2 is the principal human slicer Argonaute and cleaves
guide-complementary target RNA in RISC.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0031047
label: regulatory ncRNA-mediated gene silencing
evidence_type: IEA
original_reference_id: GO_REF:0000120
review:
summary: regulatory ncRNA-mediated gene silencing is consistent with AGO2 as
a small-RNA-loaded Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0031054
label: pre-miRNA processing
evidence_type: IEA
original_reference_id: GO_REF:0000120
review:
summary: pre-miRNA processing is supported for AGO2 because AGO2
participates in RISC maturation and AGO2-specific pre-miRNA handling such
as pre-miR-451 biology.
action: ACCEPT
reason: AGO2 is the catalytic Argonaute and has specific evidence for guide
loading, RISC maturation, and pre-miRNA-linked processing contexts.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0035197
label: siRNA binding
evidence_type: IEA
original_reference_id: GO_REF:0000104
review:
summary: siRNA binding is a core component of AGO2 RISC loading and
siRNA-guided cleavage.
action: ACCEPT
reason: AGO2 forms minimal RISC with short guide RNAs including siRNAs;
siRNA guide binding is central to AGO2's RNAi/slicer activity.
supported_by: &id002
- reference_id: file:human/AGO2/AGO2-uniprot.txt
supporting_text: The 'minimal RISC' appears to include AGO2 bound to a
short guide RNA such as a microRNA (miRNA) or short interfering RNA
(siRNA).
- term:
id: GO:0035198
label: miRNA binding
evidence_type: IEA
original_reference_id: GO_REF:0000120
review:
summary: miRNA binding is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0035278
label: miRNA-mediated gene silencing by inhibition of translation
evidence_type: IEA
original_reference_id: GO_REF:0000120
review:
summary: miRNA-mediated gene silencing by inhibition of translation is
consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0035279
label: miRNA-mediated gene silencing by mRNA destabilization
evidence_type: IEA
original_reference_id: GO_REF:0000120
review:
summary: miRNA-mediated gene silencing by mRNA destabilization is consistent
with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0036464
label: cytoplasmic ribonucleoprotein granule
evidence_type: IEA
original_reference_id: GO_REF:0000117
review:
summary: cytoplasmic ribonucleoprotein granule is consistent with AGO2 as a
small-RNA-loaded Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0045947
label: negative regulation of translational initiation
evidence_type: IEA
original_reference_id: GO_REF:0000104
review:
summary: negative regulation of translational initiation is downstream or
context-specific for AGO2.
action: KEEP_AS_NON_CORE
reason: This process may be supported in a particular experiment or pathway
model, but it is peripheral to the core Argonaute/RISC molecular role.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0070551
label: endoribonuclease activity, cleaving siRNA-paired mRNA
evidence_type: IEA
original_reference_id: GO_REF:0000002
review:
summary: endoribonuclease activity, cleaving siRNA-paired mRNA is
appropriate for AGO2 catalytic slicer activity.
action: ACCEPT
reason: AGO2 is the principal human slicer Argonaute and cleaves
guide-complementary target RNA in RISC.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0070922
label: RISC complex assembly
evidence_type: IEA
original_reference_id: GO_REF:0000117
review:
summary: RISC complex assembly is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0098808
label: mRNA cap binding
evidence_type: IEA
original_reference_id: GO_REF:0000104
review:
summary: mRNA cap binding is experimentally supported for AGO2-mediated
translational repression, but is not its central guide-loaded slicer/RISC
activity.
action: KEEP_AS_NON_CORE
reason: PMID:17524464 and UniProt support AGO2 binding to the mRNA m7G cap
as a mechanism for translational repression. This is a specific non-core
molecular function rather than a generic RNA-binding over-annotation.
supported_by: *id001
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:14749716
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:16424907
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:16756390
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:17400507
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:17891150
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:17932509
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:18178619
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:19167051
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:19324964
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:19383768
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:19716330
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:19820710
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:19838187
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:19955415
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:20671708
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:21525958
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:21840310
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:21858095
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:21903422
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:21981923
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:22366686
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:22484317
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:23085987
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:23090477
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:23622242
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:23636329
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:23661684
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:23741051
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:24043833
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:24550003
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:24981741
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:25446899
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:26496610
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:28683311
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:28877994
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:32814053
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:33961781
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:35271311
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:39251607
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0003729
label: mRNA binding
evidence_type: IEA
original_reference_id: GO_REF:0000107
review:
summary: mRNA binding is too generic for AGO2.
action: MARK_AS_OVER_ANNOTATED
reason: The informative molecular function is Argonaute small-RNA/miRNA
binding within RISC rather than a broad nucleic-acid or RNA-binding label.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0004521
label: RNA endonuclease activity
evidence_type: IEA
original_reference_id: GO_REF:0000107
review:
summary: RNA endonuclease activity is appropriate for AGO2 catalytic slicer
activity.
action: ACCEPT
reason: AGO2 is the principal human slicer Argonaute and cleaves
guide-complementary target RNA in RISC.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: IEA
original_reference_id: GO_REF:0000107
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0030425
label: dendrite
evidence_type: IEA
original_reference_id: GO_REF:0000107
review:
summary: dendrite is a plausible or reported location/context but is not the
core functional site annotation for AGO2.
action: KEEP_AS_NON_CORE
reason: The core location is cytoplasmic RISC/RNP granules; nuclear,
membrane, synaptic, exosomal, or other compartment annotations should
remain non-core unless tied to a specific curated mechanism.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0060213
label: positive regulation of nuclear-transcribed mRNA poly(A) tail
shortening
evidence_type: IEA
original_reference_id: GO_REF:0000107
review:
summary: positive regulation of nuclear-transcribed mRNA poly(A) tail
shortening is downstream or context-specific for AGO2.
action: KEEP_AS_NON_CORE
reason: This process may be supported in a particular experiment or pathway
model, but it is peripheral to the core Argonaute/RISC molecular role.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0070578
label: RISC-loading complex
evidence_type: IEA
original_reference_id: GO_REF:0000120
review:
summary: RISC-loading complex is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:1900153
label: positive regulation of nuclear-transcribed mRNA catabolic process,
deadenylation-dependent decay
evidence_type: IEA
original_reference_id: GO_REF:0000107
review:
summary: positive regulation of nuclear-transcribed mRNA catabolic process,
deadenylation-dependent decay is downstream or context-specific for AGO2.
action: KEEP_AS_NON_CORE
reason: This process may be supported in a particular experiment or pathway
model, but it is peripheral to the core Argonaute/RISC molecular role.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:1990904
label: ribonucleoprotein complex
evidence_type: IEA
original_reference_id: GO_REF:0000107
review:
summary: ribonucleoprotein complex is a plausible or reported
location/context but is not the core functional site annotation for AGO2.
action: KEEP_AS_NON_CORE
reason: The core location is cytoplasmic RISC/RNP granules; nuclear,
membrane, synaptic, exosomal, or other compartment annotations should
remain non-core unless tied to a specific curated mechanism.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0035196
label: miRNA processing
evidence_type: NAS
original_reference_id: PMID:28781232
review:
summary: miRNA processing is supported for AGO2 because AGO2 participates in
RISC maturation and AGO2-specific pre-miRNA handling such as pre-miR-451
biology.
action: ACCEPT
reason: AGO2 is the catalytic Argonaute and has specific evidence for guide
loading, RISC maturation, and pre-miRNA-linked processing contexts.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0035194
label: regulatory ncRNA-mediated post-transcriptional gene silencing
evidence_type: TAS
original_reference_id: Reactome:R-HSA-426496
review:
summary: regulatory ncRNA-mediated post-transcriptional gene silencing is
consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0036464
label: cytoplasmic ribonucleoprotein granule
evidence_type: IDA
original_reference_id: GO_REF:0000052
review:
summary: cytoplasmic ribonucleoprotein granule is consistent with AGO2 as a
small-RNA-loaded Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0004521
label: RNA endonuclease activity
evidence_type: TAS
original_reference_id: Reactome:R-HSA-426520
review:
summary: RNA endonuclease activity is appropriate for AGO2 catalytic slicer
activity.
action: ACCEPT
reason: AGO2 is the principal human slicer Argonaute and cleaves
guide-complementary target RNA in RISC.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0090128
label: regulation of synapse maturation
evidence_type: IDA
original_reference_id: PMID:29735530
review:
summary: regulation of synapse maturation is downstream or context-specific
for AGO2.
action: KEEP_AS_NON_CORE
reason: This process may be supported in a particular experiment or pathway
model, but it is peripheral to the core Argonaute/RISC molecular role.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0090128
label: regulation of synapse maturation
evidence_type: IMP
original_reference_id: PMID:29735530
review:
summary: regulation of synapse maturation is downstream or context-specific
for AGO2.
action: KEEP_AS_NON_CORE
reason: This process may be supported in a particular experiment or pathway
model, but it is peripheral to the core Argonaute/RISC molecular role.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0098794
label: postsynapse
evidence_type: IDA
original_reference_id: PMID:29735530
review:
summary: postsynapse is a plausible or reported location/context but is not
the core functional site annotation for AGO2.
action: KEEP_AS_NON_CORE
reason: The core location is cytoplasmic RISC/RNP granules; nuclear,
membrane, synaptic, exosomal, or other compartment annotations should
remain non-core unless tied to a specific curated mechanism.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0098794
label: postsynapse
evidence_type: IMP
original_reference_id: PMID:29735530
review:
summary: postsynapse is a plausible or reported location/context but is not
the core functional site annotation for AGO2.
action: KEEP_AS_NON_CORE
reason: The core location is cytoplasmic RISC/RNP granules; nuclear,
membrane, synaptic, exosomal, or other compartment annotations should
remain non-core unless tied to a specific curated mechanism.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0098978
label: glutamatergic synapse
evidence_type: IDA
original_reference_id: PMID:29735530
review:
summary: glutamatergic synapse is a plausible or reported location/context
but is not the core functional site annotation for AGO2.
action: KEEP_AS_NON_CORE
reason: The core location is cytoplasmic RISC/RNP granules; nuclear,
membrane, synaptic, exosomal, or other compartment annotations should
remain non-core unless tied to a specific curated mechanism.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0098978
label: glutamatergic synapse
evidence_type: IMP
original_reference_id: PMID:29735530
review:
summary: glutamatergic synapse is a plausible or reported location/context
but is not the core functional site annotation for AGO2.
action: KEEP_AS_NON_CORE
reason: The core location is cytoplasmic RISC/RNP granules; nuclear,
membrane, synaptic, exosomal, or other compartment annotations should
remain non-core unless tied to a specific curated mechanism.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0098808
label: mRNA cap binding
evidence_type: IDA
original_reference_id: PMID:17524464
review:
summary: mRNA cap binding is experimentally supported for AGO2-mediated
translational repression, but is not its central guide-loaded slicer/RISC
activity.
action: KEEP_AS_NON_CORE
reason: PMID:17524464 and UniProt support AGO2 binding to the mRNA m7G cap
as a mechanism for translational repression. This is a specific non-core
molecular function rather than a generic RNA-binding over-annotation.
supported_by: *id001
- term:
id: GO:0005737
label: cytoplasm
evidence_type: IDA
original_reference_id: PMID:17932509
review:
summary: cytoplasm is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0033962
label: P-body assembly
evidence_type: IDA
original_reference_id: PMID:31400113
review:
summary: P-body assembly is downstream or context-specific for AGO2.
action: KEEP_AS_NON_CORE
reason: This process may be supported in a particular experiment or pathway
model, but it is peripheral to the core Argonaute/RISC molecular role.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0035925
label: mRNA 3'-UTR AU-rich region binding
evidence_type: IDA
original_reference_id: PMID:17382880
review:
summary: mRNA 3'-UTR AU-rich region binding is too generic for AGO2.
action: MARK_AS_OVER_ANNOTATED
reason: The informative molecular function is Argonaute small-RNA/miRNA
binding within RISC rather than a broad nucleic-acid or RNA-binding label.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0045727
label: positive regulation of translation
evidence_type: IDA
original_reference_id: PMID:17382880
review:
summary: positive regulation of translation is downstream or
context-specific for AGO2.
action: KEEP_AS_NON_CORE
reason: This process may be supported in a particular experiment or pathway
model, but it is peripheral to the core Argonaute/RISC molecular role.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005654
label: nucleoplasm
evidence_type: TAS
original_reference_id: Reactome:R-HSA-5578742
review:
summary: nucleoplasm is a plausible or reported location/context but is not
the core functional site annotation for AGO2.
action: KEEP_AS_NON_CORE
reason: The core location is cytoplasmic RISC/RNP granules; nuclear,
membrane, synaptic, exosomal, or other compartment annotations should
remain non-core unless tied to a specific curated mechanism.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005654
label: nucleoplasm
evidence_type: TAS
original_reference_id: Reactome:R-HSA-5578744
review:
summary: nucleoplasm is a plausible or reported location/context but is not
the core functional site annotation for AGO2.
action: KEEP_AS_NON_CORE
reason: The core location is cytoplasmic RISC/RNP granules; nuclear,
membrane, synaptic, exosomal, or other compartment annotations should
remain non-core unless tied to a specific curated mechanism.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005654
label: nucleoplasm
evidence_type: TAS
original_reference_id: Reactome:R-HSA-5578966
review:
summary: nucleoplasm is a plausible or reported location/context but is not
the core functional site annotation for AGO2.
action: KEEP_AS_NON_CORE
reason: The core location is cytoplasmic RISC/RNP granules; nuclear,
membrane, synaptic, exosomal, or other compartment annotations should
remain non-core unless tied to a specific curated mechanism.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0035278
label: miRNA-mediated gene silencing by inhibition of translation
evidence_type: IDA
original_reference_id: PMID:23409027
review:
summary: miRNA-mediated gene silencing by inhibition of translation is
consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0016442
label: RISC complex
evidence_type: IDA
original_reference_id: PMID:19701182
review:
summary: RISC complex is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0070922
label: RISC complex assembly
evidence_type: IDA
original_reference_id: PMID:19701182
review:
summary: RISC complex assembly is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:29395067
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0016442
label: RISC complex
evidence_type: IGI
original_reference_id: PMID:31507089
review:
summary: RISC complex is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0035198
label: miRNA binding
evidence_type: IPI
original_reference_id: PMID:31507089
review:
summary: miRNA binding is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0016442
label: RISC complex
evidence_type: IGI
original_reference_id: PMID:31012336
review:
summary: RISC complex is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0035198
label: miRNA binding
evidence_type: IPI
original_reference_id: PMID:31012336
review:
summary: miRNA binding is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:26009488
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0016442
label: RISC complex
evidence_type: IPI
original_reference_id: PMID:19826008
review:
summary: RISC complex is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005634
label: nucleus
evidence_type: IDA
original_reference_id: PMID:23985560
review:
summary: nucleus is a plausible or reported location/context but is not the
core functional site annotation for AGO2.
action: KEEP_AS_NON_CORE
reason: The core location is cytoplasmic RISC/RNP granules; nuclear,
membrane, synaptic, exosomal, or other compartment annotations should
remain non-core unless tied to a specific curated mechanism.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0042985
label: negative regulation of amyloid precursor protein biosynthetic process
evidence_type: ISS
original_reference_id: GO_REF:0000024
review:
summary: negative regulation of amyloid precursor protein biosynthetic
process is downstream or context-specific for AGO2.
action: KEEP_AS_NON_CORE
reason: This process may be supported in a particular experiment or pathway
model, but it is peripheral to the core Argonaute/RISC molecular role.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:24726324
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-1606561
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-1606682
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-1912362
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-1912363
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-1912364
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-1912366
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-1912367
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-1912368
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-1912406
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-203862
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-2106614
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-2106615
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-2106625
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-210805
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-2318752
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-426464
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-426489
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-426520
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-4518575
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-5578712
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-5578744
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-5578965
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-5578966
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-5687103
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-8935864
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-8935930
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-8936058
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-8936068
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-8939129
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-8944483
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-8944522
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-8944538
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-8944569
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-8944599
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-8944632
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-8944684
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-8945709
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-8948524
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-8948536
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-8948569
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-8948582
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-8948583
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-8948594
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-8948602
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-8948612
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-8948621
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-8948623
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-8948641
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-8948651
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9011958
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9012203
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9012208
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9023909
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9023912
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9038545
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9618392
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9618486
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9624925
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9657791
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9759950
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9768616
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9768643
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9768788
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9860139
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9860160
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9860168
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9924908
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9924921
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9924968
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9925087
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9925095
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9925158
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9925159
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9925246
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9925270
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9925289
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9925320
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005829
label: cytosol
evidence_type: TAS
original_reference_id: Reactome:R-HSA-9925324
review:
summary: cytosol is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:28546213
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:25697406
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0045766
label: positive regulation of angiogenesis
evidence_type: IDA
original_reference_id: PMID:27208409
review:
summary: positive regulation of angiogenesis is downstream or
context-specific for AGO2.
action: KEEP_AS_NON_CORE
reason: This process may be supported in a particular experiment or pathway
model, but it is peripheral to the core Argonaute/RISC molecular role.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:1901165
label: positive regulation of trophoblast cell migration
evidence_type: IMP
original_reference_id: PMID:27208409
review:
summary: positive regulation of trophoblast cell migration is downstream or
context-specific for AGO2.
action: KEEP_AS_NON_CORE
reason: This process may be supported in a particular experiment or pathway
model, but it is peripheral to the core Argonaute/RISC molecular role.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0000932
label: P-body
evidence_type: IDA
original_reference_id: PMID:23125361
review:
summary: P-body is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC
effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:23125361
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0016442
label: RISC complex
evidence_type: IDA
original_reference_id: PMID:17531811
review:
summary: RISC complex is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0070578
label: RISC-loading complex
evidence_type: IDA
original_reference_id: PMID:17531811
review:
summary: RISC-loading complex is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:17531811
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:23361462
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0070062
label: extracellular exosome
evidence_type: IDA
original_reference_id: PMID:28159509
review:
summary: extracellular exosome is a plausible or reported location/context
but is not the core functional site annotation for AGO2.
action: KEEP_AS_NON_CORE
reason: The core location is cytoplasmic RISC/RNP granules; nuclear,
membrane, synaptic, exosomal, or other compartment annotations should
remain non-core unless tied to a specific curated mechanism.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0031054
label: pre-miRNA processing
evidence_type: IDA
original_reference_id: PMID:16424907
review:
summary: pre-miRNA processing is supported for AGO2 because AGO2
participates in RISC maturation and AGO2-specific pre-miRNA handling such
as pre-miR-451 biology.
action: ACCEPT
reason: AGO2 is the catalytic Argonaute and has specific evidence for guide
loading, RISC maturation, and pre-miRNA-linked processing contexts.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0070578
label: RISC-loading complex
evidence_type: IDA
original_reference_id: PMID:23661684
review:
summary: RISC-loading complex is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:24862572
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:27157137
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:23409027
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0098808
label: mRNA cap binding
evidence_type: IPI
original_reference_id: PMID:23409027
review:
summary: mRNA cap binding is experimentally supported for AGO2-mediated
translational repression, but is not its central guide-loaded slicer/RISC
activity.
action: KEEP_AS_NON_CORE
reason: PMID:17524464 and UniProt support AGO2 binding to the mRNA m7G cap
as a mechanism for translational repression. This is a specific non-core
molecular function rather than a generic RNA-binding over-annotation.
supported_by: *id001
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:15766526
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:17507929
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0045944
label: positive regulation of transcription by RNA polymerase II
evidence_type: IMP
original_reference_id: PMID:25336585
review:
summary: positive regulation of transcription by RNA polymerase II reflects
a reported nuclear or regulatory context rather than the main conserved
AGO2 function.
action: KEEP_AS_NON_CORE
reason: Argonaute paralogs have reported nuclear/regulatory interactions,
but the dominant conserved function remains small-RNA-guided
post-transcriptional repression.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0035198
label: miRNA binding
evidence_type: TAS
original_reference_id: PMID:19820710
review:
summary: miRNA binding is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0070578
label: RISC-loading complex
evidence_type: IDA
original_reference_id: PMID:19820710
review:
summary: RISC-loading complex is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0070578
label: RISC-loading complex
evidence_type: IDA
original_reference_id: PMID:15973356
review:
summary: RISC-loading complex is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0001046
label: core promoter sequence-specific DNA binding
evidence_type: IMP
original_reference_id: PMID:25336585
review:
summary: core promoter sequence-specific DNA binding reflects a reported
nuclear or regulatory context rather than the main conserved AGO2
function.
action: KEEP_AS_NON_CORE
reason: Argonaute paralogs have reported nuclear/regulatory interactions,
but the dominant conserved function remains small-RNA-guided
post-transcriptional repression.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005634
label: nucleus
evidence_type: IC
original_reference_id: PMID:25336585
review:
summary: nucleus is a plausible or reported location/context but is not the
core functional site annotation for AGO2.
action: KEEP_AS_NON_CORE
reason: The core location is cytoplasmic RISC/RNP granules; nuclear,
membrane, synaptic, exosomal, or other compartment annotations should
remain non-core unless tied to a specific curated mechanism.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0000993
label: RNA polymerase II complex binding
evidence_type: IDA
original_reference_id: PMID:25336585
review:
summary: RNA polymerase II complex binding reflects a reported nuclear or
regulatory context rather than the main conserved AGO2 function.
action: KEEP_AS_NON_CORE
reason: Argonaute paralogs have reported nuclear/regulatory interactions,
but the dominant conserved function remains small-RNA-guided
post-transcriptional repression.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:25336585
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0090624
label: endoribonuclease activity, cleaving miRNA-paired mRNA
evidence_type: IMP
original_reference_id: PMID:22795694
review:
summary: endoribonuclease activity, cleaving miRNA-paired mRNA is
appropriate for AGO2 catalytic slicer activity.
action: ACCEPT
reason: AGO2 is the principal human slicer Argonaute and cleaves
guide-complementary target RNA in RISC.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0090625
label: siRNA-mediated gene silencing by mRNA destabilization
evidence_type: IMP
original_reference_id: PMID:22795694
review:
summary: siRNA-mediated gene silencing by mRNA destabilization is consistent
with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0035196
label: miRNA processing
evidence_type: IDA
original_reference_id: PMID:23661684
review:
summary: miRNA processing is supported for AGO2 because AGO2 participates in
RISC maturation and AGO2-specific pre-miRNA handling such as pre-miR-451
biology.
action: ACCEPT
reason: AGO2 is the catalytic Argonaute and has specific evidence for guide
loading, RISC maturation, and pre-miRNA-linked processing contexts.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0031054
label: pre-miRNA processing
evidence_type: IDA
original_reference_id: PMID:19966796
review:
summary: pre-miRNA processing is supported for AGO2 because AGO2
participates in RISC maturation and AGO2-specific pre-miRNA handling such
as pre-miR-451 biology.
action: ACCEPT
reason: AGO2 is the catalytic Argonaute and has specific evidence for guide
loading, RISC maturation, and pre-miRNA-linked processing contexts.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0035196
label: miRNA processing
evidence_type: IDA
original_reference_id: PMID:19966796
review:
summary: miRNA processing is supported for AGO2 because AGO2 participates in
RISC maturation and AGO2-specific pre-miRNA handling such as pre-miR-451
biology.
action: ACCEPT
reason: AGO2 is the catalytic Argonaute and has specific evidence for guide
loading, RISC maturation, and pre-miRNA-linked processing contexts.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0003725
label: double-stranded RNA binding
evidence_type: IDA
original_reference_id: PMID:19966796
review:
summary: double-stranded RNA binding is too generic for AGO2.
action: MARK_AS_OVER_ANNOTATED
reason: The informative molecular function is Argonaute small-RNA/miRNA
binding within RISC rather than a broad nucleic-acid or RNA-binding label.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0003727
label: single-stranded RNA binding
evidence_type: IDA
original_reference_id: PMID:19966796
review:
summary: single-stranded RNA binding is too generic for AGO2.
action: MARK_AS_OVER_ANNOTATED
reason: The informative molecular function is Argonaute small-RNA/miRNA
binding within RISC rather than a broad nucleic-acid or RNA-binding label.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0016442
label: RISC complex
evidence_type: IDA
original_reference_id: PMID:19966796
review:
summary: RISC complex is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0035198
label: miRNA binding
evidence_type: IDA
original_reference_id: PMID:19966796
review:
summary: miRNA binding is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0070578
label: RISC-loading complex
evidence_type: IDA
original_reference_id: PMID:19966796
review:
summary: RISC-loading complex is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0070922
label: RISC complex assembly
evidence_type: IDA
original_reference_id: PMID:19966796
review:
summary: RISC complex assembly is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0090624
label: endoribonuclease activity, cleaving miRNA-paired mRNA
evidence_type: IDA
original_reference_id: PMID:15260970
review:
summary: endoribonuclease activity, cleaving miRNA-paired mRNA is
appropriate for AGO2 catalytic slicer activity.
action: ACCEPT
reason: AGO2 is the principal human slicer Argonaute and cleaves
guide-complementary target RNA in RISC.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0090625
label: siRNA-mediated gene silencing by mRNA destabilization
evidence_type: IDA
original_reference_id: PMID:15260970
review:
summary: siRNA-mediated gene silencing by mRNA destabilization is consistent
with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005737
label: cytoplasm
evidence_type: IDA
original_reference_id: PMID:15260970
review:
summary: cytoplasm is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0016442
label: RISC complex
evidence_type: IDA
original_reference_id: PMID:15260970
review:
summary: RISC complex is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0035198
label: miRNA binding
evidence_type: IDA
original_reference_id: PMID:15260970
review:
summary: miRNA binding is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0035279
label: miRNA-mediated gene silencing by mRNA destabilization
evidence_type: IDA
original_reference_id: PMID:15260970
review:
summary: miRNA-mediated gene silencing by mRNA destabilization is consistent
with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0070551
label: endoribonuclease activity, cleaving siRNA-paired mRNA
evidence_type: IDA
original_reference_id: PMID:15260970
review:
summary: endoribonuclease activity, cleaving siRNA-paired mRNA is
appropriate for AGO2 catalytic slicer activity.
action: ACCEPT
reason: AGO2 is the principal human slicer Argonaute and cleaves
guide-complementary target RNA in RISC.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0004521
label: RNA endonuclease activity
evidence_type: IDA
original_reference_id: PMID:18178619
review:
summary: RNA endonuclease activity is appropriate for AGO2 catalytic slicer
activity.
action: ACCEPT
reason: AGO2 is the principal human slicer Argonaute and cleaves
guide-complementary target RNA in RISC.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0031054
label: pre-miRNA processing
evidence_type: IDA
original_reference_id: PMID:18178619
review:
summary: pre-miRNA processing is supported for AGO2 because AGO2
participates in RISC maturation and AGO2-specific pre-miRNA handling such
as pre-miR-451 biology.
action: ACCEPT
reason: AGO2 is the catalytic Argonaute and has specific evidence for guide
loading, RISC maturation, and pre-miRNA-linked processing contexts.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0070578
label: RISC-loading complex
evidence_type: IDA
original_reference_id: PMID:18178619
review:
summary: RISC-loading complex is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0035197
label: siRNA binding
evidence_type: IDA
original_reference_id: PMID:19701182
qualifier: contributes_to
review:
summary: siRNA binding is a core component of AGO2 RISC loading and
siRNA-guided cleavage.
action: ACCEPT
reason: AGO2 forms minimal RISC with short guide RNAs including siRNAs;
siRNA guide binding is central to AGO2's RNAi/slicer activity.
supported_by: *id002
- term:
id: GO:0016020
label: membrane
evidence_type: HDA
original_reference_id: PMID:19946888
review:
summary: membrane is a plausible or reported location/context but is not the
core functional site annotation for AGO2.
action: KEEP_AS_NON_CORE
reason: The core location is cytoplasmic RISC/RNP granules; nuclear,
membrane, synaptic, exosomal, or other compartment annotations should
remain non-core unless tied to a specific curated mechanism.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0003723
label: RNA binding
evidence_type: HDA
original_reference_id: PMID:22658674
review:
summary: RNA binding is too generic for AGO2.
action: MARK_AS_OVER_ANNOTATED
reason: The informative molecular function is Argonaute small-RNA/miRNA
binding within RISC rather than a broad nucleic-acid or RNA-binding label.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0003723
label: RNA binding
evidence_type: HDA
original_reference_id: PMID:22681889
review:
summary: RNA binding is too generic for AGO2.
action: MARK_AS_OVER_ANNOTATED
reason: The informative molecular function is Argonaute small-RNA/miRNA
binding within RISC rather than a broad nucleic-acid or RNA-binding label.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0031047
label: regulatory ncRNA-mediated gene silencing
evidence_type: ISS
original_reference_id: GO_REF:0000024
review:
summary: regulatory ncRNA-mediated gene silencing is consistent with AGO2 as
a small-RNA-loaded Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0060213
label: positive regulation of nuclear-transcribed mRNA poly(A) tail
shortening
evidence_type: ISS
original_reference_id: GO_REF:0000024
review:
summary: positive regulation of nuclear-transcribed mRNA poly(A) tail
shortening is downstream or context-specific for AGO2.
action: KEEP_AS_NON_CORE
reason: This process may be supported in a particular experiment or pathway
model, but it is peripheral to the core Argonaute/RISC molecular role.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:1900153
label: positive regulation of nuclear-transcribed mRNA catabolic process,
deadenylation-dependent decay
evidence_type: ISS
original_reference_id: GO_REF:0000024
review:
summary: positive regulation of nuclear-transcribed mRNA catabolic process,
deadenylation-dependent decay is downstream or context-specific for AGO2.
action: KEEP_AS_NON_CORE
reason: This process may be supported in a particular experiment or pathway
model, but it is peripheral to the core Argonaute/RISC molecular role.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0000932
label: P-body
evidence_type: IDA
original_reference_id: PMID:22915799
review:
summary: P-body is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC
effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:16699599
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:22915799
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:16289642
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:20616046
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005737
label: cytoplasm
evidence_type: IDA
original_reference_id: PMID:20014101
review:
summary: cytoplasm is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0005515
label: protein binding
evidence_type: IPI
original_reference_id: PMID:19801630
review:
summary: Generic protein binding does not capture the specific
Argonaute/RISC function.
action: MARK_AS_OVER_ANNOTATED
reason: The cited interactions are best interpreted as RISC/cofactor context
for small-RNA-guided silencing, not as a standalone core function.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0035278
label: miRNA-mediated gene silencing by inhibition of translation
evidence_type: IDA
original_reference_id: PMID:19801630
review:
summary: miRNA-mediated gene silencing by inhibition of translation is
consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0045947
label: negative regulation of translational initiation
evidence_type: IDA
original_reference_id: PMID:19801630
review:
summary: negative regulation of translational initiation is downstream or
context-specific for AGO2.
action: KEEP_AS_NON_CORE
reason: This process may be supported in a particular experiment or pathway
model, but it is peripheral to the core Argonaute/RISC molecular role.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0016442
label: RISC complex
evidence_type: IDA
original_reference_id: PMID:17932509
review:
summary: RISC complex is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0000932
label: P-body
evidence_type: IDA
original_reference_id: PMID:20616046
review:
summary: P-body is consistent with AGO2 as a small-RNA-loaded Argonaute/RISC
effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0016442
label: RISC complex
evidence_type: IDA
original_reference_id: PMID:20616046
review:
summary: RISC complex is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0070551
label: endoribonuclease activity, cleaving siRNA-paired mRNA
evidence_type: IDA
original_reference_id: PMID:17524464
review:
summary: endoribonuclease activity, cleaving siRNA-paired mRNA is
appropriate for AGO2 catalytic slicer activity.
action: ACCEPT
reason: AGO2 is the principal human slicer Argonaute and cleaves
guide-complementary target RNA in RISC.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0000340
label: RNA 7-methylguanosine cap binding
evidence_type: IDA
original_reference_id: PMID:17524464
review:
summary: RNA 7-methylguanosine cap binding is experimentally supported for
AGO2-mediated translational repression, but is not the main AGO2
slicer/RISC function.
action: KEEP_AS_NON_CORE
reason: This is a specific cap-binding activity supported by PMID:17524464
and UniProt, best retained as a non-core translational-repression
mechanism.
supported_by: *id001
- term:
id: GO:0035197
label: siRNA binding
evidence_type: IDA
original_reference_id: PMID:15973356
qualifier: contributes_to
review:
summary: siRNA binding is a core component of AGO2 RISC loading and
siRNA-guided cleavage.
action: ACCEPT
reason: AGO2 forms minimal RISC with short guide RNAs including siRNAs;
siRNA guide binding is central to AGO2's RNAi/slicer activity.
supported_by: *id002
- term:
id: GO:0016442
label: RISC complex
evidence_type: IDA
original_reference_id: PMID:17671087
review:
summary: RISC complex is consistent with AGO2 as a small-RNA-loaded
Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0031054
label: pre-miRNA processing
evidence_type: IDA
original_reference_id: PMID:17671087
review:
summary: pre-miRNA processing is supported for AGO2 because AGO2
participates in RISC maturation and AGO2-specific pre-miRNA handling such
as pre-miR-451 biology.
action: ACCEPT
reason: AGO2 is the catalytic Argonaute and has specific evidence for guide
loading, RISC maturation, and pre-miRNA-linked processing contexts.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0035197
label: siRNA binding
evidence_type: IDA
original_reference_id: PMID:17495927
review:
summary: siRNA binding is a core component of AGO2 RISC loading and
siRNA-guided cleavage.
action: ACCEPT
reason: AGO2 forms minimal RISC with short guide RNAs including siRNAs;
siRNA guide binding is central to AGO2's RNAi/slicer activity.
supported_by: *id002
- term:
id: GO:0035278
label: miRNA-mediated gene silencing by inhibition of translation
evidence_type: IDA
original_reference_id: PMID:17671087
review:
summary: miRNA-mediated gene silencing by inhibition of translation is
consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0035278
label: miRNA-mediated gene silencing by inhibition of translation
evidence_type: IMP
original_reference_id: PMID:18771919
review:
summary: miRNA-mediated gene silencing by inhibition of translation is
consistent with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0035279
label: miRNA-mediated gene silencing by mRNA destabilization
evidence_type: IDA
original_reference_id: PMID:17524464
review:
summary: miRNA-mediated gene silencing by mRNA destabilization is consistent
with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0035279
label: miRNA-mediated gene silencing by mRNA destabilization
evidence_type: IMP
original_reference_id: PMID:18771919
review:
summary: miRNA-mediated gene silencing by mRNA destabilization is consistent
with AGO2 as a small-RNA-loaded Argonaute/RISC effector.
action: ACCEPT
reason: The falcon report supports AGO2 function in miRISC-mediated
small-RNA-guided repression and related RISC complexes or cytoplasmic RNP
compartments.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0045947
label: negative regulation of translational initiation
evidence_type: IDA
original_reference_id: PMID:17524464
review:
summary: negative regulation of translational initiation is downstream or
context-specific for AGO2.
action: KEEP_AS_NON_CORE
reason: This process may be supported in a particular experiment or pathway
model, but it is peripheral to the core Argonaute/RISC molecular role.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0003743
label: translation initiation factor activity
evidence_type: NAS
original_reference_id: PMID:10534406
review:
summary: translation initiation factor activity is a legacy implication of
the EIF2C name and is not supported as an Argonaute activity.
action: REMOVE
reason: Human Argonautes are RISC effector proteins, not translation
initiation factors in the canonical eIF sense.
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- term:
id: GO:0006412
label: translation
evidence_type: NAS
original_reference_id: PMID:10534406
review:
summary: translation is a legacy EIF2C-name annotation and is not supported
as a direct AGO2 biological-process annotation by this reference.
action: REMOVE
reason: PMID:10534406 supports cloning and expression of the
EIF2C1/Argonaute-family gene, not AGO2 participation in the broad
biological process of translation. Direct AGO2 translation-related
annotations should be limited to supported translational-repression terms.
supported_by:
- reference_id: PMID:10534406
supporting_text: We report the cloning and characterization of the human
eukaryotic protein translation initiation factor EIF2C1 gene.
references:
- id: GO_REF:0000002
title: Gene Ontology annotation through association of InterPro records with
GO terms
findings: []
- id: GO_REF:0000024
title: Manual transfer of experimentally-verified manual GO annotation data to
orthologs by curator judgment of sequence similarity
findings: []
- id: GO_REF:0000033
title: Annotation inferences using phylogenetic trees
findings: []
- id: GO_REF:0000052
title: Gene Ontology annotation based on curation of immunofluorescence data
findings: []
- id: GO_REF:0000104
title: Electronic Gene Ontology annotations created by transferring manual GO
annotations between related proteins based on shared sequence features
findings: []
- id: GO_REF:0000107
title: Automatic transfer of experimentally verified manual GO annotation data
to orthologs using Ensembl Compara
findings: []
- id: GO_REF:0000108
title: Automatic assignment of GO terms using logical inference, based on on
inter-ontology links
findings: []
- id: GO_REF:0000117
title: Electronic Gene Ontology annotations created by ARBA machine learning
models
findings: []
- id: GO_REF:0000120
title: Combined Automated Annotation using Multiple IEA Methods
findings: []
- id: PMID:10534406
title: 'Human eukaryotic initiation factor EIF2C1 gene: cDNA sequence, genomic organization,
localization to chromosomal bands 1p34-p35, and expression.'
findings: []
- id: PMID:14749716
title: Characterization of the interactions between mammalian PAZ PIWI domain
proteins and Dicer.
findings: []
- id: PMID:15260970
title: Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs.
findings: []
- id: PMID:15766526
title: Involvement of microRNA in AU-rich element-mediated mRNA instability.
findings: []
- id: PMID:15973356
title: TRBP recruits the Dicer complex to Ago2 for microRNA processing and
gene silencing.
findings: []
- id: PMID:16289642
title: Identification of novel argonaute-associated proteins.
findings: []
- id: PMID:16424907
title: The role of PACT in the RNA silencing pathway.
findings: []
- id: PMID:16699599
title: Human retroviral host restriction factors APOBEC3G and APOBEC3F
localize to mRNA processing bodies.
findings: []
- id: PMID:16756390
title: Translation repression in human cells by microRNA-induced gene
silencing requires RCK/p54.
findings: []
- id: PMID:17382880
title: AU-rich-element-mediated upregulation of translation by FXR1 and
Argonaute 2.
findings: []
- id: PMID:17400507
title: Identification of potential protein interactors of Lrrk2.
findings: []
- id: PMID:17495927
title: The human RNA kinase hClp1 is active on 3' transfer RNA exons and short
interfering RNAs.
findings: []
- id: PMID:17507929
title: MicroRNA silencing through RISC recruitment of eIF6.
findings: []
- id: PMID:17524464
title: An mRNA m7G cap binding-like motif within human Ago2 represses
translation.
findings: []
- id: PMID:17531811
title: RNA helicase A interacts with RISC in human cells and functions in RISC
loading.
findings: []
- id: PMID:17671087
title: Let-7 microRNA-mediated mRNA deadenylation and translational repression
in a mammalian cell-free system.
findings: []
- id: PMID:17891150
title: A conserved motif in Argonaute-interacting proteins mediates functional
interactions through the Argonaute PIWI domain.
findings: []
- id: PMID:17932509
title: Proteomic and functional analysis of Argonaute-containing mRNA-protein
complexes in human cells.
findings: []
- id: PMID:18178619
title: In vitro reconstitution of the human RISC-loading complex.
findings: []
- id: PMID:18771919
title: Importance of translation and nonnucleolytic ago proteins for on-target
RNA interference.
findings: []
- id: PMID:19167051
title: Importin 8 is a gene silencing factor that targets argonaute proteins
to distinct mRNAs.
findings: []
- id: PMID:19324964
title: The C-terminal half of human Ago2 binds to multiple GW-rich regions of
GW182 and requires GW182 to mediate silencing.
findings: []
- id: PMID:19383768
title: The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence
bound transcripts independently of Argonaute proteins.
findings: []
- id: PMID:19701182
title: An RNA-dependent RNA polymerase formed by TERT and the RMRP RNA.
findings: []
- id: PMID:19716330
title: Mammalian miRNA RISC recruits CAF1 and PABP to affect PABP-dependent
deadenylation.
findings: []
- id: PMID:19801630
title: RNA-binding motif protein 4 translocates to cytoplasmic granules and
suppresses translation via argonaute2 during muscle cell differentiation.
findings: []
- id: PMID:19820710
title: Structural insights into RNA processing by the human RISC-loading
complex.
findings: []
- id: PMID:19826008
title: An integrated approach for experimental target identification of
hypoxia-induced miR-210.
findings: []
- id: PMID:19838187
title: Ago-TNRC6 triggers microRNA-mediated decay by promoting two
deadenylation steps.
findings: []
- id: PMID:19946888
title: Defining the membrane proteome of NK cells.
findings: []
- id: PMID:19955415
title: CRM1 mediates nuclear-cytoplasmic shuttling of mature microRNAs.
findings: []
- id: PMID:19966796
title: ATP-dependent human RISC assembly pathways.
findings: []
- id: PMID:20014101
title: Mouse ZAR1-like (XM_359149) colocalizes with mRNA processing components
and its dominant-negative mutant caused two-cell-stage embryonic arrest.
findings: []
- id: PMID:20616046
title: LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for
microRNA-mediated gene silencing.
findings: []
- id: PMID:20671708
title: Pathogenic LRRK2 negatively regulates microRNA-mediated translational
repression.
findings: []
- id: PMID:21525958
title: The ribosomal protein RACK1 is required for microRNA function in both
C. elegans and humans.
findings: []
- id: PMID:21840310
title: Ago2/miRISC-mediated inhibition of CBP80/20-dependent translation and
thereby abrogation of nonsense-mediated mRNA decay require the
cap-associating activity of Ago2.
findings: []
- id: PMID:21858095
title: Nuclear pore complex protein mediated nuclear localization of dicer
protein in human cells.
findings: []
- id: PMID:21903422
title: Mapping a dynamic innate immunity protein interaction network
regulating type I interferon production.
findings: []
- id: PMID:21981923
title: GW182 proteins directly recruit cytoplasmic deadenylase complexes to
miRNA targets.
findings: []
- id: PMID:22366686
title: Senescence is an endogenous trigger for microRNA-directed
transcriptional gene silencing in human cells.
findings: []
- id: PMID:22484317
title: Human prion protein binds Argonaute and promotes accumulation of
microRNA effector complexes.
findings: []
- id: PMID:22658674
title: Insights into RNA biology from an atlas of mammalian mRNA-binding
proteins.
findings: []
- id: PMID:22681889
title: The mRNA-bound proteome and its global occupancy profile on
protein-coding transcripts.
findings: []
- id: PMID:22795694
title: Slicing-independent RISC activation requires the argonaute PAZ domain.
findings: []
- id: PMID:22915799
title: HIV-1 replication and APOBEC3 antiviral activity are not regulated by P
bodies.
findings: []
- id: PMID:23085987
title: Wig1 prevents cellular senescence by regulating p21 mRNA decay through
control of RISC recruitment.
findings: []
- id: PMID:23090477
title: Defining a new role of GW182 in maintaining miRNA stability.
findings: []
- id: PMID:23125361
title: The mammalian TRIM-NHL protein TRIM71/LIN-41 is a repressor of mRNA
function.
findings: []
- id: PMID:23361462
title: Structural insights into RISC assembly facilitated by dsRNA-binding
domains of human RNA helicase A (DHX9).
findings: []
- id: PMID:23409027
title: eIF4GI facilitates the MicroRNA-mediated gene silencing.
findings: []
- id: PMID:23622242
title: ADAR1 forms a complex with Dicer to promote microRNA processing and
RNA-induced gene silencing.
findings: []
- id: PMID:23636329
title: EGFR modulates microRNA maturation in response to hypoxia through
phosphorylation of AGO2.
findings: []
- id: PMID:23661684
title: Differential roles of human Dicer-binding proteins TRBP and PACT in
small RNA processing.
findings: []
- id: PMID:23741051
title: Hsp90 cochaperones p23 and FKBP4 physically interact with hAgo2 and
activate RNA interference-mediated silencing in mammalian cells.
findings: []
- id: PMID:23985560
title: microRNA-9 targets the long non-coding RNA MALAT1 for degradation in
the nucleus.
findings: []
- id: PMID:24043833
title: Structural features of Argonaute-GW182 protein interactions.
findings: []
- id: PMID:24550003
title: Involvement of telomerase reverse transcriptase in heterochromatin
maintenance.
findings: []
- id: PMID:24726324
title: MOV10 Is a 5' to 3' RNA helicase contributing to UPF1 mRNA target
degradation by translocation along 3' UTRs.
findings: []
- id: PMID:24862572
title: Mammalian microtubule P-body dynamics are mediated by nesprin-1.
findings: []
- id: PMID:24981741
title: MTDH-SND1 interaction is crucial for expansion and activity of
tumor-initiating cells in diverse oncogene- and carcinogen-induced mammary
tumors.
findings: []
- id: PMID:25336585
title: Cellular microRNAs up-regulate transcription via interaction with
promoter TATA-box motifs.
findings: []
- id: PMID:25446899
title: Cancer exosomes perform cell-independent microRNA biogenesis and
promote tumorigenesis.
findings: []
- id: PMID:25697406
title: Roquin binds microRNA-146a and Argonaute2 to regulate microRNA
homeostasis.
findings: []
- id: PMID:26009488
title: Clnk plays a role in TNF-alpha-induced cell death in murine
fibrosarcoma cell line L929.
findings: []
- id: PMID:26496610
title: A human interactome in three quantitative dimensions organized by
stoichiometries and abundances.
findings: []
- id: PMID:27157137
title: Post-transcriptional gene silencing activity of human GIGYF2.
findings: []
- id: PMID:27208409
title: miR-15b-AGO2 play a critical role in HTR8/SVneo invasion and in a model
of angiogenesis defects related to inflammation.
findings: []
- id: PMID:28159509
title: Human Pericardial Fluid Contains Exosomes Enriched with
Cardiovascular-Expressed MicroRNAs and Promotes Therapeutic Angiogenesis.
findings: []
- id: PMID:28546213
title: Tudor-SN-mediated endonucleolytic decay of human cell microRNAs
promotes G(1)/S phase transition.
findings: []
- id: PMID:28683311
title: Argonaute Utilization for miRNA Silencing Is Determined by
Phosphorylation-Dependent Recruitment of LIM-Domain-Containing Proteins.
findings: []
- id: PMID:28781232
title: Multivalent Recruitment of Human Argonaute by GW182.
findings: []
- id: PMID:28877994
title: Cadherin complexes recruit mRNAs and RISC to regulate epithelial cell
signaling.
findings: []
- id: PMID:29395067
title: High-Density Proximity Mapping Reveals the Subcellular Organization of
mRNA-Associated Granules and Bodies.
findings: []
- id: PMID:29735530
title: NMDA receptor-dependent dephosphorylation of serine 387 in Argonaute 2
increases its degradation and affects dendritic spine density and
maturation.
findings: []
- id: PMID:31012336
title: MicroRNA-31 Negatively Regulates Interleukin-34 Expression In Vitro.
findings: []
- id: PMID:31400113
title: KSHV RNA-binding protein ORF57 inhibits P-body formation to promote
viral multiplication by interaction with Ago2 and GW182.
findings: []
- id: PMID:31507089
title: Reciprocal regulation of miR-206 and IL-6/STAT3 pathway mediates
IL6-induced gefitinib resistance in EGFR-mutant lung cancer cells.
findings: []
- id: PMID:32814053
title: Interactome Mapping Provides a Network of Neurodegenerative Disease
Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
findings: []
- id: PMID:33961781
title: Dual proteome-scale networks reveal cell-specific remodeling of the
human interactome.
findings: []
- id: PMID:35271311
title: 'OpenCell: Endogenous tagging for the cartography of human cellular organization.'
findings: []
- id: PMID:39251607
title: Systematic identification of post-transcriptional regulatory modules.
findings: []
- id: Reactome:R-HSA-1606561
title: MIR449 microRNAs bind 3'UTR of NOTCH1 mRNA
findings: []
- id: Reactome:R-HSA-1606682
title: MIR34 microRNAs bind 3'UTR of NOTCH1 mRNA
findings: []
- id: Reactome:R-HSA-1912362
title: MIR150 microRNA binds 3'UTR of NOTCH3 mRNA
findings: []
- id: Reactome:R-HSA-1912363
title: MIR200B/C microRNAs bind NOTCH1 mRNA
findings: []
- id: Reactome:R-HSA-1912364
title: MIR181C microRNA binds 3'UTR of NOTCH4 mRNA
findings: []
- id: Reactome:R-HSA-1912366
title: MIR206 microRNA binds 3'UTR of NOTCH3 mRNA
findings: []
- id: Reactome:R-HSA-1912367
title: MIR34 microRNAs bind 3'UTR of NOTCH2 mRNA
findings: []
- id: Reactome:R-HSA-1912368
title: MIR302A microRNA binds 3'UTR of NOTCH4 mRNA
findings: []
- id: Reactome:R-HSA-1912406
title: p53 positively regulates transcription of MIR34 microRNAs
findings: []
- id: Reactome:R-HSA-203862
title: 'Dicer cleaves pre-miRNA to yield duplex miRNA'
findings: []
- id: Reactome:R-HSA-2106614
title: Duplex miRNA is loaded into Argonaute
findings: []
- id: Reactome:R-HSA-2106615
title: Removal of siRNA passenger strand
findings: []
- id: Reactome:R-HSA-2106625
title: Duplex siRNA is loaded into Argonaute
findings: []
- id: Reactome:R-HSA-210805
title: Removal of miRNA passenger strand
findings: []
- id: Reactome:R-HSA-2318752
title: miR-26A microRNAs bind PTEN mRNA
findings: []
- id: Reactome:R-HSA-426464
title: Dicer cleaves double-stranded RNA to yield double-stranded siRNA
findings: []
- id: Reactome:R-HSA-426489
title: RISC binds inexactly matching target RNAs
findings: []
- id: Reactome:R-HSA-426496
title: Post-transcriptional silencing by small RNAs
findings: []
- id: Reactome:R-HSA-426520
title: Endonucleolytic RISC hydrolyzes target RNAs
findings: []
- id: Reactome:R-HSA-4518575
title: miR-92b binds 3'UTR of NLK mRNA
findings: []
- id: Reactome:R-HSA-5578712
title: Importin-8 binds AGO2:miRNA
findings: []
- id: Reactome:R-HSA-5578742
title: AGO1,2:small RNA complexes interact with chromatin
findings: []
- id: Reactome:R-HSA-5578744
title: Importin-8 imports AGO2:miRNA into the nucleus
findings: []
- id: Reactome:R-HSA-5578965
title: TNRC6A binds AGO2:miRNA
findings: []
- id: Reactome:R-HSA-5578966
title: TNRC6A:AGO2:miRNA is transported into the nucleus
findings: []
- id: Reactome:R-HSA-5687103
title: FOXO3 regulates MIR34B,C expression
findings: []
- id: Reactome:R-HSA-8935864
title: miR-302b binds RUNX1 mRNA
findings: []
- id: Reactome:R-HSA-8935930
title: miR-18a binds RUNX1 mRNA
findings: []
- id: Reactome:R-HSA-8936058
title: miR-675 binds RUNX1 mRNA
findings: []
- id: Reactome:R-HSA-8936068
title: H19 is cleaved to produce miR-675
findings: []
- id: Reactome:R-HSA-8939129
title: miR-215 binds RUNX1 mRNA
findings: []
- id: Reactome:R-HSA-8944483
title: miR-17 microRNA binds PTEN mRNA
findings: []
- id: Reactome:R-HSA-8944522
title: miR-19a microRNA binds PTEN mRNA
findings: []
- id: Reactome:R-HSA-8944538
title: miR-22 microRNA binds PTEN mRNA
findings: []
- id: Reactome:R-HSA-8944569
title: miR-25 microRNA binds PTEN mRNA
findings: []
- id: Reactome:R-HSA-8944599
title: miR-93 microRNA binds PTEN mRNA
findings: []
- id: Reactome:R-HSA-8944632
title: miR-106 microRNAs bind PTEN mRNA
findings: []
- id: Reactome:R-HSA-8944684
title: miR-205 microRNA binds PTEN mRNA
findings: []
- id: Reactome:R-HSA-8945709
title: miR-20 microRNAs bind PTEN mRNA
findings: []
- id: Reactome:R-HSA-8948524
title: PTENP1 mRNA binds miR-19b RISC
findings: []
- id: Reactome:R-HSA-8948536
title: PTENP1 mRNA binds miR-20 RISC
findings: []
- id: Reactome:R-HSA-8948569
title: miR-19b microRNA binds PTEN mRNA
findings: []
- id: Reactome:R-HSA-8948582
title: miR-17 microRNA binds VAPA mRNA
findings: []
- id: Reactome:R-HSA-8948583
title: miR-17 microRNA binds CNOT6L mRNA
findings: []
- id: Reactome:R-HSA-8948594
title: miR-19a microRNA binds VAPA mRNA
findings: []
- id: Reactome:R-HSA-8948602
title: miR-19a microRNA binds CNOT6L mRNA
findings: []
- id: Reactome:R-HSA-8948612
title: miR-19b microRNA binds CNOT6L mRNA
findings: []
- id: Reactome:R-HSA-8948621
title: miR-20 microRNAs bind VAPA mRNA
findings: []
- id: Reactome:R-HSA-8948623
title: miR-20 microRNAs bind CNOT6L mRNA
findings: []
- id: Reactome:R-HSA-8948641
title: miR-106 microRNAs bind VAPA mRNA
findings: []
- id: Reactome:R-HSA-8948651
title: miR-106a,(miR106b) microRNA binds CNOT6L mRNA
findings: []
- id: Reactome:R-HSA-9011958
title: miR-26A and B bind to the 3'UTR of the GREB1 mRNA
findings: []
- id: Reactome:R-HSA-9012203
title: miR-26A and B bind to the 3'UTR of the CHD11 mRNA
findings: []
- id: Reactome:R-HSA-9012208
title: miR-26A and B bind to the 3'UTR of the KPNA2 mRNA
findings: []
- id: Reactome:R-HSA-9023909
title: C3PO hydrolyzes cleaved passenger strand
findings: []
- id: Reactome:R-HSA-9023912
title: AGO2 cleaves passenger strand of duplex siRNA
findings: []
- id: Reactome:R-HSA-9038545
title: miR-613 binds to the 3'UTR of the NR1H3 mRNA
findings: []
- id: Reactome:R-HSA-9618392
title: miR-26 binds to the 3'UTR of the ARL4C mRNA
findings: []
- id: Reactome:R-HSA-9618486
title: miR-26 binds to the 3'UTR of the ABCA1 mRNA
findings: []
- id: Reactome:R-HSA-9624925
title: miR-33 binds to the 3'UTR of the ABCA1 mRNA
findings: []
- id: Reactome:R-HSA-9657791
title: miR-144 binds to the 3'UTR of the ABCA1 mRNA
findings: []
- id: Reactome:R-HSA-9759950
title: miR-200c-3p binds CDH11 mRNA
findings: []
- id: Reactome:R-HSA-9768616
title: miR-9 binds CDH1 mRNA
findings: []
- id: Reactome:R-HSA-9768643
title: miR-10b binds CDH1 mRNA
findings: []
- id: Reactome:R-HSA-9768788
title: miR-224 binds NPAS4 mRNA
findings: []
- id: Reactome:R-HSA-9860139
title: MIR27B microRNA binds 3'UTR of TGFBR3 mRNA
findings: []
- id: Reactome:R-HSA-9860160
title: MIR-Let7-a1 microRNA binds 3'UTR of TGFBR3 mRNA
findings: []
- id: Reactome:R-HSA-9860168
title: MIR23B microRNA binds 3'UTR of TGFBR3 mRNA
findings: []
- id: Reactome:R-HSA-9924908
title: CD274 mRNA binds miR-93 RISC
findings: []
- id: Reactome:R-HSA-9924921
title: CD274 mRNA binds miR-34 RISC
findings: []
- id: Reactome:R-HSA-9924968
title: CD274 mRNA binds miR-429 RISC
findings: []
- id: Reactome:R-HSA-9925087
title: CD274 mRNA binds miR-138-5p RISC
findings: []
- id: Reactome:R-HSA-9925095
title: CD274 mRNA binds miR-340 RISC
findings: []
- id: Reactome:R-HSA-9925158
title: CD274 mRNA binds miR-152 RISC
findings: []
- id: Reactome:R-HSA-9925159
title: CD274 mRNA binds miR-140 RISC
findings: []
- id: Reactome:R-HSA-9925246
title: CD274 mRNA binds miR-Let7-a1 RISC
findings: []
- id: Reactome:R-HSA-9925270
title: CD274 mRNA binds miR-148a-3p RISC
findings: []
- id: Reactome:R-HSA-9925289
title: CD274 mRNA binds miR-424 RISC
findings: []
- id: Reactome:R-HSA-9925320
title: CD274 mRNA binds miR-142-5p RISC
findings: []
- id: Reactome:R-HSA-9925324
title: CD274 mRNA binds miR-200B/C RISC
findings: []
- id: file:human/AGO2/AGO2-uniprot.txt
title: UniProt text export for AGO2
findings:
- statement: UniProt identifies AGO2 and its protein family/domain context.
supporting_text: Belongs to the argonaute family. Ago subfamily.
- id: file:human/AGO2/AGO2-deep-research-falcon.md
title: Falcon deep research report for AGO2
findings:
- statement: Falcon research supports the reviewed core function of AGO2.
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
core_functions:
- description: RNA-guided target cleavage by catalytic AGO2 RISC loaded with
miRNA or siRNA guides.
molecular_function:
id: GO:0004521
label: RNA endonuclease activity
directly_involved_in:
- id: GO:0090625
label: siRNA-mediated gene silencing by mRNA destabilization
- id: GO:0035279
label: miRNA-mediated gene silencing by mRNA destabilization
in_complex:
id: GO:0016442
label: RISC complex
locations:
- id: GO:0005737
label: cytoplasm
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
- description: Cleavage-independent miRISC repression through small-RNA binding
and effector recruitment.
molecular_function:
id: GO:0035198
label: miRNA binding
directly_involved_in:
- id: GO:0035194
label: regulatory ncRNA-mediated post-transcriptional gene silencing
- id: GO:0035278
label: miRNA-mediated gene silencing by inhibition of translation
in_complex:
id: GO:0016442
label: RISC complex
locations:
- id: GO:0000932
label: P-body
- id: GO:0005829
label: cytosol
supported_by:
- reference_id: file:human/AGO2/AGO2-deep-research-falcon.md
supporting_text: Human AGO2 cleaves guide-complementary target RNA at the
phosphodiester bond between target positions t10 and t11.
proposed_new_terms: []
suggested_questions:
- question: Which endogenous human tissues or stress states require
AGO2-specific RISC activity rather than redundant activity from other AGO
paralogs?
- question: Which protein cofactors determine guide loading, localization, and
non-core nuclear or granule-associated functions of AGO2?
suggested_experiments:
- description: Endogenous tagging of AGO2 followed by small-RNA CLIP, target RNA
profiling, and paralog-specific rescue in AGO-depleted human cells.
experiment_type: genome editing/RNA-protein interaction profiling
hypothesis: AGO2 has paralog-specific guide, target, and compartment
preferences within human RISC biology.
- description: Biochemical reconstitution of AGO2-RISC with representative miRNA
and siRNA guides to compare target repression, target cleavage, and TNRC6
recruitment.
experiment_type: biochemical reconstitution
hypothesis: AGO2 activity is best explained by guide-loaded RISC function
rather than generic RNA or protein binding.