AGTRAP (ATRAP) is a small, ubiquitously expressed integral membrane protein of 159 amino acids with three transmembrane helices, an extracellular/lumenal N-terminus and a roughly 50-residue cytoplasmic C-terminal tail. It sits in endoplasmic reticulum, Golgi and endocytic vesicle membranes and at the plasma membrane, and its vesicles move constitutively toward the cell surface. Its defining activity is binding the carboxy-terminal cytoplasmic tail of the type-1 angiotensin II receptor (AGTR1) through its own C-terminal tail; the interaction is selective for AT1 and does not extend to the AT2, m3 muscarinic, bradykinin B2, endothelin B or beta2-adrenergic receptors. Through it AGTRAP restrains angiotensin II signalling: it promotes AT1 receptor internalization and lowers receptor density at the cell surface, and cells over-expressing it show reduced phospholipase C activation, inositol lipid generation, c-fos induction and proliferation in response to angiotensin II. Mice lacking Atrap have elevated arterial blood pressure, expanded plasma volume and increased surface AT1 receptor in the renal cortex, where the protein is most abundant in the proximal tubule brush border; human AGTRAP mRNA is highest in kidney, heart, pancreas and thyroid. In heart, AGTRAP additionally binds the sarcoplasmic reticulum calcium pump SERCA2a and stimulates its calcium uptake, accelerating cytosolic calcium decay and ventricular relaxation - an activity independent of the angiotensin receptor. Further reported partners include the scaffold RACK1, the phosphatidylinositol transfer protein PITPNC1/RdgB-beta, which AGTRAP recruits to membranes, and FAM114A1. AGTRAP is itself neither a receptor nor an enzyme: it has no G-protein coupling and no catalytic activity.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0000139 Golgi membrane | IEA GO_REF:0000044 | ACCEPT | Summary: Correct. UniProt's Golgi apparatus membrane SUBCELLULAR LOCATION line for AGTRAP carries ECO:0000269|PubMed:12960423, so this keyword-derived row traces to a direct microscopy result on the protein rather than to a prediction. Reason: PubMed:12960423 localised ATRAP by immunofluorescence and electron microscopy to perinuclear vesicular membranes corresponding to endoplasmic reticulum, Golgi and endocytic vesicles, and this is the experimental basis of the three UniProt SubCell terms. The protein is a multi-pass membrane protein (three predicted TM helices, UniProt FT 24-44, 56-76, 87-107), so a membrane-qualified location is the right granularity. Supporting Evidence: PMID:12960423 colocalization analysis by immunofluorescence shows that ATRAP colocalizes in an intracellular vesicular compartment corresponding to endoplasmic reticulum, Golgi, and endocytic vesicles |
| GO:0001666 response to hypoxia | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Over-annotated. The whole annotation rests on a single rat IEP - a transcript abundance change in a neonatal-brain hypoxic-preconditioning experiment - projected into human by orthology. Reason: The donor was resolved and then its own evidence queried rather than assumed: UniProtKB:Q642A2 is rat Agtrap (ATRAP_RAT, Swiss-Prot, 160 aa, a true orthologue), and its only experimental support for GO:0001666 is one IEP from PubMed:17033689, 'Vascular response to hypoxic preconditioning in the immature brain'. Querying QuickGO by that reference returns two annotations in all of GOA - rat Agtrap and rat Angptl4 - both IEP, i.e. the paper's GO footprint is an expression-pattern readout and nothing more. An expression change formally satisfies 'response to' in the source species, but projecting a tissue- and development-stage-specific response term across species asserts something about human AGTRAP that has never been tested. Kept rather than removed because the rat observation itself is not in doubt. Propagation Review Root cause: SOURCE WEAK OR INFERRED Failure modes: SOURCE EVIDENCE WEAK CONTEXT OR TISSUE MISMATCH Sources checked: UniProtKB:Q642A2 · rat Agtrap (ATRAP_RAT) SOURCE WEAK OR INFERRED True orthologue, but holds GO:0001666 by a single IEP from PubMed:17033689, which annotates only 2 entities in all of GOA. ensembl:ENSRNOP00000011747 · rat Agtrap protein SOURCE WEAK OR INFERRED Compara's protein handle on the same single IEP. |
| GO:0004945 angiotensin type II receptor activity | IEA GO_REF:0000107 | REMOVE | Summary: Wrong, and demonstrably so in two independent ways. AGTRAP is not a receptor, and the gene's founding paper specifically tested and excluded any AT2 interaction; the annotation traces to an MGI row taken from a paper about Agtr2, not Agtrap. Reason: Biology first, because the argument does not depend on provenance. GO:0004945 is defined as 'An angiotensin receptor activity that acts via Gi protein coupling and cGMP (NO) generation', and the qualifier here is enables - i.e. the assertion is that AGTRAP is a functional AT2 receptor. AGTRAP is a 159-residue protein with three transmembrane helices (UniProt FT 24-44, 56-76, 87-107), not a seven-pass GPCR, and nothing in its record involves G-protein coupling. PubMed:10358057 assayed exactly this question and reported that ATRAP binds the AT1a C-terminal tail and specifically not AT2, so the term is contradicted by direct experiment on this protein, and it names the wrong receptor subtype for a protein whose own name is 'type-1'. Provenance second, and it explains how the row arose. The Compara donor UniProtKB:Q9WVK0 is mouse Agtrap, a true orthologue, but its GO:0004945 is an MGI IMP citing PubMed:10425188 - 'Increased vasoconstrictor response of the mouse lacking angiotensin II type 2 receptor', an Agtr2-knockout study whose abstract does not mention Agtrap. Querying QuickGO by reference rather than by gene shows that paper annotating three entities: Agt, Agtr2 (which correctly receives GO:0004945 enables by IGI) and Agtrap - two alphabetically adjacent symbols, one of which is an angiotensin receptor. The error is now in three species: rat Agtrap (Q642A2) carries GO:0004945 both by Ensembl IEA and by RGD ISO. Correcting the MGI row would clear all of them. Propagation Review Root cause: SOURCE BAD Failure modes: SOURCE MISCITATION ROLE CONFLATION FUNCTIONAL DIVERGENCE Sources checked: UniProtKB:Q9WVK0 · mouse Agtrap (ATRAP_MOUSE) SOURCE BAD Reviewed 1:1 orthologue, so the orthology is fine; its GO:0004945 IMP cites PubMed:10425188, an Agtr2-knockout paper that assays no Agtrap allele. Probable Agtr2/Agtrap symbol confusion. ensembl:ENSMUSP00000030865 · mouse Agtrap protein SOURCE BAD Compara's protein-level handle on the same defective MGI row. UniProtKB:P35374 · mouse Agtr2 NOT RELEVANT The gene PubMed:10425188 actually characterises; it holds GO:0004945 enables by IGI from the same paper. Supporting Evidence: PMID:10358057 ATRAP interacts specifically with the carboxyl-terminal domain of the AT1a receptor but not with those of angiotensin II type 2 (AT2), m3 muscarinic acetylcholine, bradykinin B2, endothelin B, and beta2-adrenergic receptors. PMID:10425188 In the mice lacking the gene encoding the AT2 receptor, the pressor response to Ang II was increased although the underlying mechanism is unknown. file:human/AGTRAP/AGTRAP-uniprot.txt FT TRANSMEM 87..107 |
| GO:0005515 protein binding | IPI PMID:16189514 Towards a proteome-scale map of the human protein-protein in... | KEEP AS NON CORE | Summary: PBX3 is a two-hybrid hit later corroborated by co-immunoprecipitation in breast cancer cells, where AGTRAP directs USP14-mediated stabilisation of PBX3. Real but context-restricted, and the accession used is not canonical PBX3. Reason: PMID:35414770 independently co-immunoprecipitates ATRAP with PBX3 in two breast cancer lines and shows ATRAP over-expression raises PBX3 protein without changing its mRNA. Two caveats keep this non-core. First, the assay is over-expression in a cancer context and PBX3 is a nuclear homeodomain factor, whereas AGTRAP is a three-pass membrane protein - the compartments do not obviously meet. Second, the GOA row cites Q96AL5, an unreviewed 367-aa TrEMBL entry, not canonical PBX3 (P40426, 434 aa), so the clone assayed is not the full-length protein. Kept rather than removed because an orthogonal assay exists, which is more than the rest of the screen set has. Supporting Evidence: PMID:35414770 we performed co-IP assays in MDA-MB-453 and T47D cells transfected with Flag-tagged ATRAP, which revealed that ATRAP can directly bind with PBX3 |
| GO:0005515 protein binding | IPI PMID:16189514 Towards a proteome-scale map of the human protein-protein in... | MODIFY | Summary: PITPNC1 (RdgB-beta) is one of only three AGTRAP two-hybrid partners with orthogonal support, and the only one that yields an informative molecular function for AGTRAP itself: a targeted study shows AGTRAP recruits the PITP domain of RdgB-beta to membranes, i.e. AGTRAP is the membrane anchor. Reason: The partner is real. PMID:21728994 is titled for RdgB-beta rather than for AGTRAP, which is why no AGTRAP-keyed search surfaces it; it reports that on PMA treatment the PITP domain of RdgB-beta engages AGTRAP and is thereby recruited to membranes. That is exactly GO:0043495 protein-membrane adaptor activity - 'brings together a protein ... with a membrane ... to establish or maintain the localization of the protein' - which integral membrane tethers such as SUN1, SUN2 and GPR179 already carry. Bare protein binding states nothing about what AGTRAP does, so the row is modified rather than accepted. Two caveats, stated rather than left for a reader to find. First, GO:0043495's differentia describes the adaptor as reaching the membrane 'either via membrane lipid binding or by interacting with a membrane protein', and AGTRAP does neither - it is itself the integral membrane protein. GO's usage is broader than that wording: SUN1, SUN2 and GPR179 all hold the term and are all integral membrane proteins tethering a partner at a membrane, which is exactly this case, so the term fits practice even where the differentia reads narrowly. Second, the recruitment rests on a single paper, and the underlying GOA evidence here is still two-hybrid (PMID:16189514, PMID:19060904, PMID:25416956, PMID:32296183, 4 CCSB releases of one clone set). This activity is deliberately kept out of core_functions: a single PMA-dependent recruitment experiment, with no consequence of the recruitment measured for AGTRAP itself and no evidence that it happens at endogenous levels, is a real molecular function but not a demonstrated core one. It is recorded here, where it was measured, rather than promoted. Proposed replacements: protein-membrane adaptor activity Supporting Evidence: PMID:21728994 interacts with the integral membrane protein ATRAP (angiotensin II type I receptor-associated protein; also known as AGTRAP) causing membrane recruitment |
| GO:0005515 protein binding | IPI PMID:19060904 An empirical framework for binary interactome mapping. | KEEP AS NON CORE | Summary: PBX3 is a two-hybrid hit later corroborated by co-immunoprecipitation in breast cancer cells, where AGTRAP directs USP14-mediated stabilisation of PBX3. Real but context-restricted, and the accession used is not canonical PBX3. Reason: PMID:35414770 independently co-immunoprecipitates ATRAP with PBX3 in two breast cancer lines and shows ATRAP over-expression raises PBX3 protein without changing its mRNA. Two caveats keep this non-core. First, the assay is over-expression in a cancer context and PBX3 is a nuclear homeodomain factor, whereas AGTRAP is a three-pass membrane protein - the compartments do not obviously meet. Second, the GOA row cites Q96AL5, an unreviewed 367-aa TrEMBL entry, not canonical PBX3 (P40426, 434 aa), so the clone assayed is not the full-length protein. Kept rather than removed because an orthogonal assay exists, which is more than the rest of the screen set has. Supporting Evidence: PMID:35414770 we performed co-IP assays in MDA-MB-453 and T47D cells transfected with Flag-tagged ATRAP, which revealed that ATRAP can directly bind with PBX3 |
| GO:0005515 protein binding | IPI PMID:19060904 An empirical framework for binary interactome mapping. | MODIFY | Summary: PITPNC1 (RdgB-beta) is one of only three AGTRAP two-hybrid partners with orthogonal support, and the only one that yields an informative molecular function for AGTRAP itself: a targeted study shows AGTRAP recruits the PITP domain of RdgB-beta to membranes, i.e. AGTRAP is the membrane anchor. Reason: The partner is real. PMID:21728994 is titled for RdgB-beta rather than for AGTRAP, which is why no AGTRAP-keyed search surfaces it; it reports that on PMA treatment the PITP domain of RdgB-beta engages AGTRAP and is thereby recruited to membranes. That is exactly GO:0043495 protein-membrane adaptor activity - 'brings together a protein ... with a membrane ... to establish or maintain the localization of the protein' - which integral membrane tethers such as SUN1, SUN2 and GPR179 already carry. Bare protein binding states nothing about what AGTRAP does, so the row is modified rather than accepted. Two caveats, stated rather than left for a reader to find. First, GO:0043495's differentia describes the adaptor as reaching the membrane 'either via membrane lipid binding or by interacting with a membrane protein', and AGTRAP does neither - it is itself the integral membrane protein. GO's usage is broader than that wording: SUN1, SUN2 and GPR179 all hold the term and are all integral membrane proteins tethering a partner at a membrane, which is exactly this case, so the term fits practice even where the differentia reads narrowly. Second, the recruitment rests on a single paper, and the underlying GOA evidence here is still two-hybrid (PMID:16189514, PMID:19060904, PMID:25416956, PMID:32296183, 4 CCSB releases of one clone set). This activity is deliberately kept out of core_functions: a single PMA-dependent recruitment experiment, with no consequence of the recruitment measured for AGTRAP itself and no evidence that it happens at endogenous levels, is a real molecular function but not a demonstrated core one. It is recorded here, where it was measured, rather than promoted. Proposed replacements: protein-membrane adaptor activity Supporting Evidence: PMID:21728994 interacts with the integral membrane protein ATRAP (angiotensin II type I receptor-associated protein; also known as AGTRAP) causing membrane recruitment |
| GO:0005515 protein binding | IPI PMID:21516116 Next-generation sequencing to generate interactome datasets. | MARK AS OVER ANNOTATED | Summary: PDHX (O00330) appears only as a yeast two-hybrid hit, in 3 CCSB interactome releases (PMID:21516116, PMID:25416956, PMID:31515488) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. PDHX is confined to the mitochondrial matrix, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:21516116 Next-generation sequencing to generate interactome datasets. | MARK AS OVER ANNOTATED | Summary: PTPN9 (P43378) appears only as a yeast two-hybrid hit, in 4 CCSB interactome releases (PMID:21516116, PMID:25416956, PMID:31515488, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:21516116 Next-generation sequencing to generate interactome datasets. | MARK AS OVER ANNOTATED | Summary: ARFIP2 (P53365) appears only as a yeast two-hybrid hit, in 3 CCSB interactome releases (PMID:21516116, PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:21516116 Next-generation sequencing to generate interactome datasets. | MARK AS OVER ANNOTATED | Summary: SPG21 (Q9NZD8) appears only as a yeast two-hybrid hit, in 4 CCSB interactome releases (PMID:21516116, PMID:25416956, PMID:31515488, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: SUCLA2 (E5KS60) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. The accession used, E5KS60, is an unreviewed (TrEMBL) entry rather than the reviewed record for this gene. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: PDHX (O00330) appears only as a yeast two-hybrid hit, in 3 CCSB interactome releases (PMID:21516116, PMID:25416956, PMID:31515488) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. PDHX is confined to the mitochondrial matrix, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: SSX5 (O60225) appears only as a yeast two-hybrid hit, in 4 CCSB interactome releases (PMID:25416956, PMID:29892012, PMID:31515488, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: FARS2 (O95363) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. FARS2 is confined to the mitochondrial matrix, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: CD160 (O95971) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: CAPNS1 (P04632) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: HMGB1 (P09429) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: NFYB (P25208) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. NFYB is confined to the nucleus, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: LMO2 (P25791) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. LMO2 is confined to the nucleus, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: HSPA4 (P34932) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: GDNF (P39905) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. GDNF is confined to the extracellular space, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: PTPN9 (P43378) appears only as a yeast two-hybrid hit, in 4 CCSB interactome releases (PMID:21516116, PMID:25416956, PMID:31515488, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: HARS2 (P49590) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: ARFIP2 (P53365) appears only as a yeast two-hybrid hit, in 3 CCSB interactome releases (PMID:21516116, PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: ALDH18A1 (P54886) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. ALDH18A1 is confined to the mitochondrial matrix, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: GABARAPL2 (P60520) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: TUBA1B (P68363) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: TFAM (Q00059) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. TFAM is confined to the mitochondrial matrix, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: GAD2 (Q05329) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: GTF3C1 (Q12789) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. GTF3C1 is confined to the nucleus, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: SNX1 (Q13596) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: FASTKD3 (Q14CZ7) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: NKG7 (Q16617) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: SYT16 (Q17RD7) appears only as a yeast two-hybrid hit, in 4 CCSB interactome releases (PMID:25416956, PMID:29892012, PMID:31515488, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | KEEP AS NON CORE | Summary: PBX3 is a two-hybrid hit later corroborated by co-immunoprecipitation in breast cancer cells, where AGTRAP directs USP14-mediated stabilisation of PBX3. Real but context-restricted, and the accession used is not canonical PBX3. Reason: PMID:35414770 independently co-immunoprecipitates ATRAP with PBX3 in two breast cancer lines and shows ATRAP over-expression raises PBX3 protein without changing its mRNA. Two caveats keep this non-core. First, the assay is over-expression in a cancer context and PBX3 is a nuclear homeodomain factor, whereas AGTRAP is a three-pass membrane protein - the compartments do not obviously meet. Second, the GOA row cites Q5JS98, an unreviewed 455-aa TrEMBL entry, not canonical PBX3 (P40426, 434 aa), so the clone assayed is not the full-length protein. Kept rather than removed because an orthogonal assay exists, which is more than the rest of the screen set has. Supporting Evidence: PMID:35414770 we performed co-IP assays in MDA-MB-453 and T47D cells transfected with Flag-tagged ATRAP, which revealed that ATRAP can directly bind with PBX3 |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: ECPAS (Q5VYK3) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: MRM1 (Q6IN84) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. MRM1 is confined to the mitochondrial matrix, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: PTPN9 (Q6IQ43) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. The accession used, Q6IQ43, is an unreviewed (TrEMBL) entry rather than the reviewed record for this gene. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: STAG3L1 (Q7L1H2) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. The accession used, Q7L1H2, is an unreviewed (TrEMBL) entry rather than the reviewed record for this gene. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: GAD1 (Q8IVA8) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. The accession used, Q8IVA8, is an unreviewed (TrEMBL) entry rather than the reviewed record for this gene. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | KEEP AS NON CORE | Summary: FAM114A1 is a two-hybrid hit that a later targeted study took up and confirmed in native tissue, placing the AGTRAP-FAM114A1 pair upstream of AT1R abundance in cardiac fibroblasts. Kept, but protein binding is not a core function. Reason: PMID:35671117 is titled for FAM114A1, not for AGTRAP, and states outright that it began from the published two-hybrid candidates and then validated this one by immunoprecipitation and Western blotting in mouse heart lysates and primary cardiac fibroblasts - i.e. an orthogonal assay at endogenous levels, which none of the other screen partners has. Loss of Fam114a1 raised AGTRAP protein and lowered AT1R, and siRNA knockdown of Agtrap reversed the Fam114a1-null phenotype, so the interaction is functional as well as physical. Retained as non-core because GO:0005515 itself conveys no molecular function and the regulatory direction runs from FAM114A1 onto AGTRAP rather than the reverse. Supporting Evidence: PMID:35671117 we validated the interaction between AGTRAP and FAM114A1 in mouse heart lysates and cultured PMCFs by IP and Western blotting |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: HSCB (Q8IWL3) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:31515488) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: FHIP1B (Q8N612) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: DDX55 (Q8NHQ9) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. DDX55 is confined to the nucleoplasm, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: COQ8A (Q8NI60) appears only as a yeast two-hybrid hit, in 4 CCSB interactome releases (PMID:25416956, PMID:25910212, PMID:31515488, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: LNX1 (Q8TBB1) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: FATE1 (Q969F0) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: TBRG4 (Q969Z0) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. TBRG4 is confined to the mitochondrial matrix, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: MIEF2 (Q96C03) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: ACSF2 (Q96CM8) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:31515488) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: BPIFA2 (Q96DR5) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. BPIFA2 is confined to the extracellular space, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: MTERF3 (Q96E29) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: ARAF (Q96II5) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. The accession used, Q96II5, is an unreviewed (TrEMBL) entry rather than the reviewed record for this gene. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: TPD52L3 (Q96J77) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: RMDN2 (Q96LZ7) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: TCEANC2 (Q96MN5) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. TCEANC2 is confined to the nucleus, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: MCEE (Q96PE7) appears only as a yeast two-hybrid hit, in 4 CCSB interactome releases (PMID:25416956, PMID:25910212, PMID:31515488, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: GAD1 (Q99259) appears only as a yeast two-hybrid hit, in 3 CCSB interactome releases (PMID:25416956, PMID:26871637, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: H4C7 (Q99525) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. H4C7 is confined to the nucleus and chromosome, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: TXN2 (Q99757) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: SSX3 (Q99909) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: ZFYVE21 (Q9BQ24) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: PIMREG (Q9BSJ6) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:26871637) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. PIMREG is confined to the nucleus and nucleolus, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: DDA1 (Q9BW61) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: ISCU (Q9H1K1) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: MYG1 (Q9HB07) appears only as a yeast two-hybrid hit, in 3 CCSB interactome releases (PMID:25416956, PMID:31515488, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. MYG1 is confined to the nucleoplasm and mitochondrial matrix, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: SENP2 (Q9HC62) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: MIEF1 (Q9NQG6) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: SPG21 (Q9NZD8) appears only as a yeast two-hybrid hit, in 4 CCSB interactome releases (PMID:21516116, PMID:25416956, PMID:31515488, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: CRCT1 (Q9UGL9) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: MUTYH (Q9UIF7) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. MUTYH is confined to the nucleus and mitochondrion, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MODIFY | Summary: PITPNC1 (RdgB-beta) is one of only three AGTRAP two-hybrid partners with orthogonal support, and the only one that yields an informative molecular function for AGTRAP itself: a targeted study shows AGTRAP recruits the PITP domain of RdgB-beta to membranes, i.e. AGTRAP is the membrane anchor. Reason: The partner is real. PMID:21728994 is titled for RdgB-beta rather than for AGTRAP, which is why no AGTRAP-keyed search surfaces it; it reports that on PMA treatment the PITP domain of RdgB-beta engages AGTRAP and is thereby recruited to membranes. That is exactly GO:0043495 protein-membrane adaptor activity - 'brings together a protein ... with a membrane ... to establish or maintain the localization of the protein' - which integral membrane tethers such as SUN1, SUN2 and GPR179 already carry. Bare protein binding states nothing about what AGTRAP does, so the row is modified rather than accepted. Two caveats, stated rather than left for a reader to find. First, GO:0043495's differentia describes the adaptor as reaching the membrane 'either via membrane lipid binding or by interacting with a membrane protein', and AGTRAP does neither - it is itself the integral membrane protein. GO's usage is broader than that wording: SUN1, SUN2 and GPR179 all hold the term and are all integral membrane proteins tethering a partner at a membrane, which is exactly this case, so the term fits practice even where the differentia reads narrowly. Second, the recruitment rests on a single paper, and the underlying GOA evidence here is still two-hybrid (PMID:16189514, PMID:19060904, PMID:25416956, PMID:32296183, 4 CCSB releases of one clone set). This activity is deliberately kept out of core_functions: a single PMA-dependent recruitment experiment, with no consequence of the recruitment measured for AGTRAP itself and no evidence that it happens at endogenous levels, is a real molecular function but not a demonstrated core one. It is recorded here, where it was measured, rather than promoted. Proposed replacements: protein-membrane adaptor activity Supporting Evidence: PMID:21728994 interacts with the integral membrane protein ATRAP (angiotensin II type I receptor-associated protein; also known as AGTRAP) causing membrane recruitment |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: PCTP (Q9UKL6) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: NDRG4 (Q9ULP0) appears only as a yeast two-hybrid hit, in 3 CCSB interactome releases (PMID:25416956, PMID:26871637, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: NFU1 (Q9UMS0) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:25416956) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: AP3M1 (Q9Y2T2) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:25910212 Widespread macromolecular interaction perturbations in human... | MARK AS OVER ANNOTATED | Summary: COQ8A (Q8NI60) appears only as a yeast two-hybrid hit, in 4 CCSB interactome releases (PMID:25416956, PMID:25910212, PMID:31515488, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:25910212 Widespread macromolecular interaction perturbations in human... | MARK AS OVER ANNOTATED | Summary: MCEE (Q96PE7) appears only as a yeast two-hybrid hit, in 4 CCSB interactome releases (PMID:25416956, PMID:25910212, PMID:31515488, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:25910212 Widespread macromolecular interaction perturbations in human... | MARK AS OVER ANNOTATED | Summary: PNPO (Q9NVS9) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25910212, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:26871637 Widespread Expansion of Protein Interaction Capabilities by ... | MARK AS OVER ANNOTATED | Summary: FGA (A0A0S2Z3E9) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:26871637) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. The accession used is unreviewed (TrEMBL) A0A0S2Z3E9, 84 aa, against a canonical FGA of 866 aa, so the construct assayed is not the full-length protein. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:26871637 Widespread Expansion of Protein Interaction Capabilities by ... | MARK AS OVER ANNOTATED | Summary: GAD1 (A0A0S2Z4D9) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:26871637) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. The accession used is unreviewed (TrEMBL) A0A0S2Z4D9, 111 aa, against a canonical GAD1 of 594 aa, so the construct assayed is not the full-length protein. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:26871637 Widespread Expansion of Protein Interaction Capabilities by ... | MARK AS OVER ANNOTATED | Summary: S100B (A8MRB1) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:26871637) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. The accession used, A8MRB1, is an unreviewed (TrEMBL) entry rather than the reviewed record for this gene. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:26871637 Widespread Expansion of Protein Interaction Capabilities by ... | MARK AS OVER ANNOTATED | Summary: GAD1 (Q99259) appears only as a yeast two-hybrid hit, in 3 CCSB interactome releases (PMID:25416956, PMID:26871637, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:26871637 Widespread Expansion of Protein Interaction Capabilities by ... | MARK AS OVER ANNOTATED | Summary: PIMREG (Q9BSJ6) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:26871637) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. PIMREG is confined to the nucleus and nucleolus, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:26871637 Widespread Expansion of Protein Interaction Capabilities by ... | MARK AS OVER ANNOTATED | Summary: DIABLO (Q9NR28) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:26871637, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:26871637 Widespread Expansion of Protein Interaction Capabilities by ... | MARK AS OVER ANNOTATED | Summary: SUCLA2 (Q9P2R7) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:26871637, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:26871637 Widespread Expansion of Protein Interaction Capabilities by ... | MARK AS OVER ANNOTATED | Summary: NDRG4 (Q9ULP0) appears only as a yeast two-hybrid hit, in 3 CCSB interactome releases (PMID:25416956, PMID:26871637, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:29892012 An interactome perturbation framework prioritizes damaging m... | MARK AS OVER ANNOTATED | Summary: SSX5 (O60225) appears only as a yeast two-hybrid hit, in 4 CCSB interactome releases (PMID:25416956, PMID:29892012, PMID:31515488, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:29892012 An interactome perturbation framework prioritizes damaging m... | MARK AS OVER ANNOTATED | Summary: SYT16 (Q17RD7) appears only as a yeast two-hybrid hit, in 4 CCSB interactome releases (PMID:25416956, PMID:29892012, PMID:31515488, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:31515488 Extensive disruption of protein interactions by genetic vari... | MARK AS OVER ANNOTATED | Summary: PDHX (O00330) appears only as a yeast two-hybrid hit, in 3 CCSB interactome releases (PMID:21516116, PMID:25416956, PMID:31515488) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. PDHX is confined to the mitochondrial matrix, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:31515488 Extensive disruption of protein interactions by genetic vari... | MARK AS OVER ANNOTATED | Summary: SSX5 (O60225) appears only as a yeast two-hybrid hit, in 4 CCSB interactome releases (PMID:25416956, PMID:29892012, PMID:31515488, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:31515488 Extensive disruption of protein interactions by genetic vari... | MARK AS OVER ANNOTATED | Summary: PTPN9 (P43378) appears only as a yeast two-hybrid hit, in 4 CCSB interactome releases (PMID:21516116, PMID:25416956, PMID:31515488, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:31515488 Extensive disruption of protein interactions by genetic vari... | MARK AS OVER ANNOTATED | Summary: SYT16 (Q17RD7) appears only as a yeast two-hybrid hit, in 4 CCSB interactome releases (PMID:25416956, PMID:29892012, PMID:31515488, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:31515488 Extensive disruption of protein interactions by genetic vari... | MARK AS OVER ANNOTATED | Summary: HSCB (Q8IWL3) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:31515488) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:31515488 Extensive disruption of protein interactions by genetic vari... | MARK AS OVER ANNOTATED | Summary: COQ8A (Q8NI60) appears only as a yeast two-hybrid hit, in 4 CCSB interactome releases (PMID:25416956, PMID:25910212, PMID:31515488, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:31515488 Extensive disruption of protein interactions by genetic vari... | MARK AS OVER ANNOTATED | Summary: ACSF2 (Q96CM8) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:31515488) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:31515488 Extensive disruption of protein interactions by genetic vari... | MARK AS OVER ANNOTATED | Summary: MCEE (Q96PE7) appears only as a yeast two-hybrid hit, in 4 CCSB interactome releases (PMID:25416956, PMID:25910212, PMID:31515488, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:31515488 Extensive disruption of protein interactions by genetic vari... | MARK AS OVER ANNOTATED | Summary: MYG1 (Q9HB07) appears only as a yeast two-hybrid hit, in 3 CCSB interactome releases (PMID:25416956, PMID:31515488, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. MYG1 is confined to the nucleoplasm and mitochondrial matrix, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI PMID:31515488 Extensive disruption of protein interactions by genetic vari... | MARK AS OVER ANNOTATED | Summary: SPG21 (Q9NZD8) appears only as a yeast two-hybrid hit, in 4 CCSB interactome releases (PMID:21516116, PMID:25416956, PMID:31515488, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: HSBP1L1 (C9JCN9) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: MGST3 (O14880) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: FFAR2 (O15552) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: SSX5 (O60225) appears only as a yeast two-hybrid hit, in 4 CCSB interactome releases (PMID:25416956, PMID:29892012, PMID:31515488, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: KCNK5 (O95279) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: FARS2 (O95363) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. FARS2 is confined to the mitochondrial matrix, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: CYB5R3 (P00387) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: APOA4 (P06727) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. APOA4 is confined to the extracellular space, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: CELA3A (P09093) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: CD79A (P11912) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: IL7R (P16871) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: PPIF (P30405) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. PPIF is confined to the mitochondrial matrix, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: HIBADH (P31937) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: GTF2H1 (P32780) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. GTF2H1 is confined to the nucleus, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: PTPN9 (P43378) appears only as a yeast two-hybrid hit, in 4 CCSB interactome releases (PMID:21516116, PMID:25416956, PMID:31515488, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: KIR2DL3 (P43628) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: PCYT1A (P49585) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: TTPA (P49638) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: STAR (P49675) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: ARFIP2 (P53365) appears only as a yeast two-hybrid hit, in 3 CCSB interactome releases (PMID:21516116, PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: SLC18A1 (P54219) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: ATP5F1E (P56381) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: SCAND1 (P57086) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. SCAND1 is confined to the nucleus, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: TFAM (Q00059) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. TFAM is confined to the mitochondrial matrix, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: GAD2 (Q05329) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: AQP6 (Q13520) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: MMD (Q15546) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: TCEA2 (Q15560) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. TCEA2 is confined to the nucleus, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: RAB30 (Q15771) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: NKG7 (Q16617) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: STX1A (Q16623) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: SYT16 (Q17RD7) appears only as a yeast two-hybrid hit, in 4 CCSB interactome releases (PMID:25416956, PMID:29892012, PMID:31515488, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: FAM209A (Q5JX71) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: ECPAS (Q5VYK3) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: ANKS6 (Q68DC2) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: MRM1 (Q6IN84) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. MRM1 is confined to the mitochondrial matrix, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: ETFRF1 (Q6IPR1) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: CCDC70 (Q6NSX1) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: TMPPE (Q6ZT21) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: UBE2I (Q7KZS0) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. UBE2I is confined to the nucleus, which that 52-residue cytosolic surface cannot reach in an intact cell. The accession used, Q7KZS0, is an unreviewed (TrEMBL) entry rather than the reviewed record for this gene. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: HSD17B13 (Q7Z5P4) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: CPLX4 (Q7Z7G2) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: RETREG3 (Q86VR2) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: TMEM139 (Q8IV31) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: RBFA (Q8N0V3) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: KIR3DL3 (Q8N743) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: COQ8A (Q8NI60) appears only as a yeast two-hybrid hit, in 4 CCSB interactome releases (PMID:25416956, PMID:25910212, PMID:31515488, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: LNX1 (Q8TBB1) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: SLC7A14 (Q8TBB6) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: FNDC9 (Q8TBE3) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: GPR152 (Q8TDT2) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: GPX8 (Q8TED1) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: THAP4 (Q8WY91) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: FATE1 (Q969F0) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: TBRG4 (Q969Z0) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. TBRG4 is confined to the mitochondrial matrix, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: LRRC59 (Q96AG4) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: PBX3 is a two-hybrid hit later corroborated by co-immunoprecipitation in breast cancer cells, where AGTRAP directs USP14-mediated stabilisation of PBX3. Real but context-restricted, and the accession used is not canonical PBX3. Reason: PMID:35414770 independently co-immunoprecipitates ATRAP with PBX3 in two breast cancer lines and shows ATRAP over-expression raises PBX3 protein without changing its mRNA. Two caveats keep this non-core. First, the assay is over-expression in a cancer context and PBX3 is a nuclear homeodomain factor, whereas AGTRAP is a three-pass membrane protein - the compartments do not obviously meet. Second, the GOA row cites Q96AL5, an unreviewed 367-aa TrEMBL entry, not canonical PBX3 (P40426, 434 aa), so the clone assayed is not the full-length protein. Kept rather than removed because an orthogonal assay exists, which is more than the rest of the screen set has. Supporting Evidence: PMID:35414770 we performed co-IP assays in MDA-MB-453 and T47D cells transfected with Flag-tagged ATRAP, which revealed that ATRAP can directly bind with PBX3 |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: MIEF2 (Q96C03) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: STARD4 (Q96DR4) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: BPIFA2 (Q96DR5) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. BPIFA2 is confined to the extracellular space, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: MRRF (Q96E11) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: MTERF3 (Q96E29) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: DNAJC1 (Q96KC8) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: RMDN2 (Q96LZ7) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: MCEE (Q96PE7) appears only as a yeast two-hybrid hit, in 4 CCSB interactome releases (PMID:25416956, PMID:25910212, PMID:31515488, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: GAD1 (Q99259) appears only as a yeast two-hybrid hit, in 3 CCSB interactome releases (PMID:25416956, PMID:26871637, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: PRPF18 (Q99633) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. PRPF18 is confined to the nuclear speckle, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: TXN2 (Q99757) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: CYBC1 (Q9BQA9) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: NDUFAF3 (Q9BU61) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: BCL2L13 (Q9BXK5) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: SMG9 (Q9H0W8) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: STMN4 (Q9H169) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: MYG1 (Q9HB07) appears only as a yeast two-hybrid hit, in 3 CCSB interactome releases (PMID:25416956, PMID:31515488, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. MYG1 is confined to the nucleoplasm and mitochondrial matrix, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: SENP2 (Q9HC62) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: LRRC4C (Q9HCJ2) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: MIEF1 (Q9NQG6) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: DIABLO (Q9NR28) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:26871637, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: PNPO (Q9NVS9) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25910212, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: SPG21 (Q9NZD8) appears only as a yeast two-hybrid hit, in 4 CCSB interactome releases (PMID:21516116, PMID:25416956, PMID:31515488, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: SUCLA2 (Q9P2R7) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:26871637, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: HSFX1 (Q9UBD0) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: PITPNC1 (RdgB-beta) is one of only three AGTRAP two-hybrid partners with orthogonal support, and the only one that yields an informative molecular function for AGTRAP itself: a targeted study shows AGTRAP recruits the PITP domain of RdgB-beta to membranes, i.e. AGTRAP is the membrane anchor. Reason: The partner is real. PMID:21728994 is titled for RdgB-beta rather than for AGTRAP, which is why no AGTRAP-keyed search surfaces it; it reports that on PMA treatment the PITP domain of RdgB-beta engages AGTRAP and is thereby recruited to membranes. That is exactly GO:0043495 protein-membrane adaptor activity - 'brings together a protein ... with a membrane ... to establish or maintain the localization of the protein' - which integral membrane tethers such as SUN1, SUN2 and GPR179 already carry. Bare protein binding states nothing about what AGTRAP does, so the row is modified rather than accepted. Two caveats, stated rather than left for a reader to find. First, GO:0043495's differentia describes the adaptor as reaching the membrane 'either via membrane lipid binding or by interacting with a membrane protein', and AGTRAP does neither - it is itself the integral membrane protein. GO's usage is broader than that wording: SUN1, SUN2 and GPR179 all hold the term and are all integral membrane proteins tethering a partner at a membrane, which is exactly this case, so the term fits practice even where the differentia reads narrowly. Second, the recruitment rests on a single paper, and the underlying GOA evidence here is still two-hybrid (PMID:16189514, PMID:19060904, PMID:25416956, PMID:32296183, 4 CCSB releases of one clone set). This activity is deliberately kept out of core_functions: a single PMA-dependent recruitment experiment, with no consequence of the recruitment measured for AGTRAP itself and no evidence that it happens at endogenous levels, is a real molecular function but not a demonstrated core one. It is recorded here, where it was measured, rather than promoted. Proposed replacements: protein-membrane adaptor activity Supporting Evidence: PMID:21728994 interacts with the integral membrane protein ATRAP (angiotensin II type I receptor-associated protein; also known as AGTRAP) causing membrane recruitment |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: NDRG4 (Q9ULP0) appears only as a yeast two-hybrid hit, in 3 CCSB interactome releases (PMID:25416956, PMID:26871637, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: ERGIC3 (Q9Y282) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: POLDIP2 (Q9Y2S7) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. POLDIP2 is confined to the mitochondrial matrix and nucleus, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: AP3M1 (Q9Y2T2) appears only as a yeast two-hybrid hit, in 2 CCSB interactome releases (PMID:25416956, PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: YARS2 (Q9Y2Z4) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. YARS2 is confined to the mitochondrial matrix, which that 52-residue cytosolic surface cannot reach in an intact cell. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: SH3GLB1 (Q9Y371) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005515 protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: MRPL11 (Q9Y3B7) appears only as a yeast two-hybrid hit, in 1 CCSB interactome release (PMID:32296183) drawn from one clone set and one assay. No orthogonal experiment supports it and protein binding conveys no molecular function, so the row is marked over-annotated rather than treated as evidence about AGTRAP. Reason: All 188 GOA binding rows for AGTRAP come from nine CCSB binary-interactome publications that share one ORFeome clone collection and one yeast two-hybrid system. IntAct holds 617 evidences for Q6RW13, of which 583 (94.5%) are two-hybrid; PMID:32296183 contributes 276 = 92 pairs logged under exactly three sub-method terms (validated two hybrid / two hybrid array / two hybrid prey pooling approach) and PMID:25416956 contributes 202 = 68/67/67 the same way, so UniProt's NbExp counts sub-methods, not experiments. Only 23 of 213 distinct IntAct pairs carry any non-two-hybrid method, and none of those 23 is in GOA. Both partners are over-expressed as fusions in the yeast nucleus, whereas AGTRAP is a three-pass ER/Golgi/vesicle membrane protein whose only substantial cytosol-facing surface is residues 108-159 - 52 residues, 20 of them disordered. IntAct holds two-hybrid methods only for this pair. That is not an impression: across all 617 evidences, every one of AGTRAP's 132 GOA partners is two-hybrid-only, and the 23 AGTRAP pairs that do carry AP-MS, co-immunoprecipitation or BioID support are disjoint from the GOA set. No targeted study of this partner with AGTRAP appears in the affinage record or in UniProt's curated SUBUNIT and FUNCTION lines either. That is the distinction drawn across this gene's binding rows: PITPNC1, FAM114A1 and PBX3 each have an orthogonal assay; this pair has none. MARK_AS_OVER_ANNOTATED rather than REMOVE, matching the treatment of comparable unreplicated CCSB rows in the merged ADAMTSL5 review (and deliberately diverging from ADAMTSL4, which used REMOVE). |
| GO:0005789 endoplasmic reticulum membrane | IEA GO_REF:0000044 | ACCEPT | Summary: Correct. UniProt's endoplasmic reticulum membrane SUBCELLULAR LOCATION line for AGTRAP carries ECO:0000269|PubMed:12960423, so this keyword-derived row traces to a direct microscopy result on the protein rather than to a prediction. Reason: PubMed:12960423 localised ATRAP by immunofluorescence and electron microscopy to perinuclear vesicular membranes corresponding to endoplasmic reticulum, Golgi and endocytic vesicles, and this is the experimental basis of the three UniProt SubCell terms. The protein is a multi-pass membrane protein (three predicted TM helices, UniProt FT 24-44, 56-76, 87-107), so a membrane-qualified location is the right granularity. Supporting Evidence: PMID:12960423 colocalization analysis by immunofluorescence shows that ATRAP colocalizes in an intracellular vesicular compartment corresponding to endoplasmic reticulum, Golgi, and endocytic vesicles |
| GO:0005794 Golgi apparatus | IDA GO_REF:0000052 | ACCEPT | Summary: Independent immunofluorescence confirmation of the Golgi localisation, from the Human Protein Atlas rather than from the UniProt keyword route. Reason: This is the only IDA in AGTRAP's entire GO record and it agrees with the experimental Golgi localisation curated from PubMed:12960423. It is less precise than the GO:0000139 Golgi membrane row already present, but it is independent evidence for the same compartment rather than a redundant transfer, so it is kept as annotated. |
| GO:0005886 plasma membrane | IBA GO_REF:0000033 | ACCEPT | Summary: Sound. The single PAINT node carrying this term is seeded from mouse Agtrap, whose own evidence is an IDA at the same term, and the human protein is independently reported at the plasma membrane. Reason: PTHR16521 has exactly two IBD annotations family-wide and this is one of them (node PTN002314244, seed MGI:MGI:1339977, taxon:6072). Querying the donor's own evidence rather than only its identity: mouse Agtrap (Q9WVK0) holds GO:0005886 by IDA from PubMed:10358057, assigned by UniProt - so the propagation does not land above its donor and no downward MODIFY is warranted (the ACRV1 failure mode was checked for and is absent). PubMed:12960423 independently reports the human protein at the plasma membrane with constitutive vesicular translocation towards it, which is also where AGTR1 engagement must occur, so is_active_in is apt. Propagation Review Root cause: NO FAILURE CORE Sources checked: MGI:MGI:1339977 · mouse Agtrap SUPPORTS TRANSFER Holds GO:0005886 by IDA from PubMed:10358057; a true 1:1 orthologue, not a paralogue - PTHR16521 contains no paralogues. PANTHER:PTN002314244 · PTHR16521 Eumetazoa node SUPPORTS TRANSFER Internal tree node, not a protein; one of only two IBD annotations in the whole family. Supporting Evidence: PMID:12960423 Real-time tracking of ATRAP vesicles shows constitutive translocation toward the plasma membrane. |
| GO:0005886 plasma membrane | IEA GO_REF:0000107 | ACCEPT | Summary: Redundant with the IBA row but independently sound: the Compara donor is mouse Agtrap, which holds plasma membrane by its own IDA. Reason: The donor UniProtKB:Q9WVK0 was resolved (ATRAP_MOUSE, Swiss-Prot, 161 aa) and its own annotations queried: GO:0005886 located_in by IDA from PubMed:10358057. Unlike the GO:0004945 row projected from the same donor, this one rests on evidence that is about Agtrap. Kept as annotated. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:Q9WVK0 · mouse Agtrap (ATRAP_MOUSE) SUPPORTS TRANSFER Reviewed (Swiss-Prot) 1:1 orthologue; carries GO:0005886 located_in by IDA from PubMed:10358057. |
| GO:0005938 cell cortex | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Over-annotated. The donor's own IDA is a light-microscopy peripheral-staining call on mouse Agtrap; for an integral multi-pass membrane protein the plasma membrane term already annotated is the apt one, and cell cortex adds a sub-membranous cytoplasmic claim the protein does not occupy. Reason: The donor was resolved and its evidence queried: mouse Agtrap (Q9WVK0) holds GO:0005938 located_in by IDA from PubMed:10358057, the same fluorescence-microscopy experiment that supports its plasma membrane IDA. GO:0005938 is defined as 'the region of a cell that lies just beneath the plasma membrane', typically an actin-rich cytoplasmic zone. AGTRAP has three transmembrane helices and a 52-residue cytoplasmic tail; it is in the membrane, not in the cortical cytoplasm, and nothing in the human record (UniProt SUBCELLULAR LOCATION lists ER, Golgi and cytoplasmic vesicle membranes only) supports a distinct cortical pool. Marked over-annotated rather than removed because the underlying microscopy is real and peripheral staining is compatible with the plasma membrane row that is retained. Propagation Review Root cause: TERM SCOPING PROBLEM Failure modes: COMPARTMENT OR COMPLEX MISMATCH GRANULARITY MISMATCH Sources checked: UniProtKB:Q9WVK0 · mouse Agtrap (ATRAP_MOUSE) SUPPORTS SOURCE BUT NOT TARGET Holds GO:0005938 by IDA from PubMed:10358057; the same experiment also supports its plasma membrane IDA, which is the term that should transfer. |
| GO:0008217 regulation of blood pressure | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: The term is right and the propagation is sound, but its only experimental donor cites the wrong paper. Retained as non-core organism-level physiology, rescued by knockout evidence that nobody has curated. Reason: Chain traced end to end. The human IBA comes from PANTHER node PTN002703102, one of only two IBD annotations in PTHR16521, seeded from MGI:MGI:1339977; mouse Agtrap's sole experimental GO:0008217 row is an IMP citing PubMed:10425188, the Agtr2-knockout paper identified on the GO:0004945 row above. So the donor's citation is defective. The conclusion nonetheless holds on independent evidence that GO has not captured: Atrap-deficient mice have significantly higher mean systolic blood pressure, higher plasma volume and increased renal-cortical AT1R surface expression (PubMed:20093357). Blood-pressure regulation is an organism-level consequence of restraining AT1R signalling rather than AGTRAP's own molecular activity, so it is kept as non-core. Recorded as a request to MGI to re-reference the mouse row rather than as a reason to drop the human one. Propagation Review Root cause: NO FAILURE NON CORE Failure modes: SOURCE MISCITATION Sources checked: MGI:MGI:1339977 · mouse Agtrap SOURCE BAD Its GO:0008217 IMP cites PubMed:10425188, an Agtr2-knockout study. The term is nevertheless true of mouse Agtrap on PubMed:20093357, which is uncurated. PANTHER:PTN002703102 · PTHR16521 Euteleostomi node SUPPORTS TRANSFER Internal tree node; the family has no paralogues, so the transfer itself is orthologue-to-orthologue. Supporting Evidence: PMID:20093357 Mean systolic BP was significantly higher in Atrap-/- mice compared with wild-type mice. |
| GO:0030659 cytoplasmic vesicle membrane | IEA GO_REF:0000044 | ACCEPT | Summary: Correct. UniProt's cytoplasmic vesicle membrane SUBCELLULAR LOCATION line for AGTRAP carries ECO:0000269|PubMed:12960423, so this keyword-derived row traces to a direct microscopy result on the protein rather than to a prediction. Reason: PubMed:12960423 localised ATRAP by immunofluorescence and electron microscopy to perinuclear vesicular membranes corresponding to endoplasmic reticulum, Golgi and endocytic vesicles, and this is the experimental basis of the three UniProt SubCell terms. The protein is a multi-pass membrane protein (three predicted TM helices, UniProt FT 24-44, 56-76, 87-107), so a membrane-qualified location is the right granularity. Supporting Evidence: PMID:12960423 colocalization analysis by immunofluorescence shows that ATRAP colocalizes in an intracellular vesicular compartment corresponding to endoplasmic reticulum, Golgi, and endocytic vesicles |
| GO:0038166 angiotensin-activated signaling pathway | IEA GO_REF:0000002 | MODIFY | Summary: The sign is wrong. This is an InterPro family-name mapping asserting that AGTRAP is part of the angiotensin-activated pathway, whereas every measurement on the protein shows it damping that pathway. Reason: The row comes from InterPro:IPR009436, the AGTRAP family signature, so the term derives from the family's name rather than from any experiment - the same name-implies-function shape as the GO:0004945 row. What has actually been measured runs the other way: ATRAP over-expression markedly inhibits AT1a-mediated phospholipase C activation while leaving m3 receptor signalling intact (PubMed:10358057), reduces inositol lipid generation, angiotensin-II-stimulated c-fos promoter activity and proliferation (PubMed:12960423), and Atrap-null mice show elevated blood pressure with increased surface AT1R (PubMed:20093357). GO keeps regulation terms outside the process they regulate, so the correct term is GO:0110062 negative regulation of angiotensin-activated signaling pathway. Modified rather than removed because the biology the signature was reaching for is real; only its direction and role are wrong. Propagation Review Root cause: TERM SCOPING PROBLEM Failure modes: ROLE CONFLATION REGULATORY SIGN INVERSION Sources checked: InterPro:IPR009436 · AGTRAP family signature SUPPORTS SOURCE BUT NOT TARGET A family-name-derived interpro2go mapping; the family is single-subfamily and AGTRAP-specific, so the signature is correct - the GO term attached to it is not. Proposed replacements: negative regulation of angiotensin-activated signaling pathway Supporting Evidence: PMID:10358057 Overexpression of ATRAP in COS-7 cells caused a marked inhibition of AT1a receptor-mediated activation of phospholipase C without affecting m3 receptor-mediated activation. PMID:12960423 a moderate decrease in the generation of inositol lipids, a marked decrease in the angiotensin II-stimulated transcriptional activity of the c-fos promoter luciferase reporter gene, and a decrease in cell proliferation |
| GO:0042802 identical protein binding | IPI Q6RW13-2 PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: A self-interaction recorded only by the CCSB two-hybrid pipeline - on isoform 2 in HuRI, and on the canonical protein in two earlier releases - with no orthogonal test of AGTRAP oligomerisation anywhere. Reason: The GOA row rests on PMID:32296183, where Q6RW13-2 was paired with itself, and IntAct logs that one screen under three sub-method names, so its apparent replicate count is an artefact of the sub-method vocabulary rather than of independent assays. IntAct does record the self-pair more than once - PMID:16189514 and PMID:25416956 also report a canonical Q6RW13 self-pair - but all three are the same CCSB clone collection in the same two-hybrid system, so they are recurrence on one platform rather than replication across methods. Homodimerisation of a three-pass membrane protein is plausible a priori but has never been tested by crosslinking, co-immunoprecipitation of differentially tagged copies, or any size-based method, and the tested species is isoform 2, which lacks residues 115-121 from within the AGTR1-interaction region 110-122. Marked over-annotated rather than removed: the observation stands, its weight does not. |
| GO:0031702 type 1 angiotensin receptor binding | IPI PMID:12960423 The angiotensin II type I receptor-associated protein, ATRAP... | NEW | Summary: Proposed. This is the interaction the gene is named for, it is measured, UniProt curates it on the human entry and gives it a sequence feature, and the mouse orthologue carries it in GO - yet human GOA has no trace of it. Reason: AGTRAP was isolated as a binder of the AT1a receptor's carboxy-terminal cytoplasmic domain, and the binding is subtype-selective: PubMed:10358057 tested AT2, m3 muscarinic, bradykinin B2, endothelin B and beta2-adrenergic tails and found none of them bound. PubMed:12960423 mapped the requirement to AGTRAP's own C-terminal tail by deletion, and UniProt records the interaction on the human entry (SUBUNIT with ECO:0000269) plus a REGION 110..122 feature annotated 'Interaction with AGTR1'. The mouse orthologue already carries this as GO:0005515 IPI with P29754 (Agtr1a) from PubMed:10358057; the human gene carries 187 protein binding rows, none of them AGTR1. GO:0031702 is chosen over its parent GO:0031701 because the measured fact is precisely the subtype selectivity. The term is already established for proteins that bind the receptor's cytoplasmic face rather than its ligand pocket, so this is not a novel use of it: of its 197 annotations over 188 gene products, 16 entities hold it by non-IEA evidence, and those include ARRB2, JAK2, TYK2 and ARAP1, each by rat IPI, alongside AGT (the ligand precursor; human IPI, mouse IDA). What is thin is human coverage - five human entities, of which only AGT and BDKRB2 are IPI and the other three are Ensembl projections - and none of them is an accessory protein of AGTRAP's kind. Evidence code, stated with its difficulty rather than asserted. IPI on PubMed:12960423 rests on two things. First, UniProt tags that paper with ECO:0000269 on the human entry for FUNCTION, SUBUNIT and SUBCELLULAR LOCATION - a curator judgement made with the full text, which this review does not have (PMC284805 returns no extractable body). Second, NLM indexes PubMed:12960423 with the MeSH organism term 'Humans' and with neither 'Animals' nor 'Mice', whereas the founding mouse paper PubMed:10358057 is indexed 'Animals' + 'Mice' and not 'Humans'. The indexing discriminates the two papers, and the absence of 'Animals' is hard to account for if the experiments had used mouse material. Against that sits a real discrepancy that should not be buried: the abstract numbers the protein's hydrophobic domains 14-36, 55-77 and 88-108 with a cytoplasmic tail at 109-161, and 161 aa is mouse Agtrap (Q9WVK0), not human Q6RW13 (159 aa, TOPO_DOM 108..159). The likeliest reconciliation is that the authors kept the residue numbering of the sequence first published in PubMed:10358057 while working with human constructs, but that cannot be confirmed without the full text. IPI is therefore retained on explicit deference to the UniProt curator, with the numbering conflict recorded; if the full text shows rodent constructs, the correct code is ISS with UniProtKB:Q9WVK0 and the supporting entity becomes the murine AT1a receptor UniProtKB:P29754 rather than human AGTR1 UniProtKB:P30556 - the code and the partner accession change together, and the term does not change either way. The founding characterisation (PubMed:10358057) used the mouse protein and mouse AT1a and is cited as corroboration rather than as the human evidence. Supporting Evidence: PMID:12960423 Mutant forms of ATRAP lacking the carboxyl end are unable to bind to the AT1 receptor, leading to the formation of prominent perinuclear vesicle clusters. PMID:10358057 ATRAP interacts specifically with the carboxyl-terminal domain of the AT1a receptor but not with those of angiotensin II type 2 (AT2), m3 muscarinic acetylcholine, bradykinin B2, endothelin B, and beta2-adrenergic receptors. file:human/AGTRAP/AGTRAP-uniprot.txt CC -!- SUBUNIT: Interacts with RACK1, and with the C-terminal region of AGTR1. file:human/AGTRAP/AGTRAP-uniprot.txt FT /note="Interaction with AGTR1" |
| GO:0002092 positive regulation of receptor internalization | ISS PMID:11162453 ATRAP, novel AT1 receptor associated protein, enhances inter... | NEW | Summary: Proposed. Promoting AT1 receptor internalization is the mechanism UniProt names in AGTRAP's FUNCTION line and the one every downstream phenotype is explained by, and GO records nothing like it. Reason: Over-expression of ATRAP potentiates angiotensin-II-stimulated AT1 receptor internalization in vascular smooth muscle cells (PubMed:11162453) and lowers AT1 receptor number at the cardiomyocyte surface (PubMed:15757644), and the corresponding loss-of-function result is that Atrap-null mice show enhanced surface AT1 receptor in the renal cortex (PubMed:20093357). UniProt's human FUNCTION line states the mechanism directly - a negative regulator acting 'by regulating receptor internalization'. The evidence code is ISS rather than IMP on the same test applied to the GO:0031702 row, and here it comes out the other way: NLM indexes PubMed:11162453 with 'Animals' and 'Rats' and no 'Humans' term, and PubMed:15757644 with 'Animals' and 'Mice' and no 'Humans'. Both are rodent over-expression experiments, so the claim for the human protein is made by orthology to mouse Agtrap (Q9WVK0) and is deliberately not asserted as a direct human observation. Proposed in addition to, not instead of, the GO:0110062 replacement on the InterPro row: that term states the outcome, this one states how it is achieved. Supporting Evidence: PMID:11162453 Transfection of ATRAP potentiated AT1 receptor internalization upon Ang II stimulation in these VSMCs. PMID:20093357 (125)I-AngII binding showed enhanced surface expression of AT1 receptors in the renal cortex of Atrap-/- mice, accompanied by increased carboanhydrase-sensitive proximal tubular function. file:human/AGTRAP/AGTRAP-uniprot.txt CC -!- FUNCTION: Appears to be a negative regulator of type-1 angiotensin II |
| GO:0141109 transporter activator activity | ISS PMID:27015675 The angiotensin receptor-associated protein Atrap is a stimu... | NEW | Summary: Proposed. A second, AT1-receptor-independent molecular activity: AGTRAP binds the cardiac calcium pump SERCA2a and increases its calcium uptake. Absent from GO in every form. Reason: PubMed:27015675 is titled for SERCA2a rather than for AGTRAP, which is why an AGTRAP-keyed search does not surface it. It identifies SERCA2a in Atrap pull-downs by MALDI-MS, confirms the interaction by co-immunoprecipitation and by surface plasmon resonance, and shows Atrap increases SERCA-dependent calcium uptake in isolated sarcoplasmic reticulum vesicles; Atrap-null myocytes have prolonged calcium-transient decay and sarcomere re-lengthening, and the mice a markedly reduced maximum ventricular filling rate with unchanged ejection fraction. GO:0141109 'binds to and increases the activity of a transporter' is the sibling of GO:0141110, which phospholamban holds by IDA for the inhibitory version of the same regulation, so the ontology already models this relationship. The knockout physiology is unambiguously mouse, hence ISS with mouse Agtrap as the supporting entity rather than IDA. One caveat in the other direction, recorded so it can be revisited: NLM indexes PubMed:27015675 with 'Humans' as well as 'Animals', and with 'HEK293 Cells', so the binding measurements may have used human constructs even though the calcium-uptake and knockout work did not. ISS is the conservative reading; confirming human constructs would license IPI for the interaction half. Stated as one well-controlled study, not as settled biology. Supporting Evidence: PMID:27015675 Sequencing by MALDI-MS of the isolated complexes showed that Atrap interacts with the cardiac Ca(2+)-ATPase SERCA2a. PMID:27015675 Atrap enhanced the SERCA-dependent Ca(2+) uptake in isolated SR membrane vesicles. |
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Download this section (compressed HTML)Q: MGI annotation request: mouse Agtrap (MGI:1339977) carries GO:0004945 angiotensin type II receptor activity (enables, IMP) and GO:0008217 (IMP) from PMID:10425188, which is an Agtr2-knockout study that assays no Agtrap allele; Agtr2 correctly receives GO:0004945 from the same paper. Retiring the GO:0004945 row would also clear the Ensembl projections onto human AGTRAP and rat Agtrap and the ISO copy RGD makes from it - one edit fixing four annotations in three species. The GO:0008217 row should be re-referenced to PMID:20093357 rather than retired, since the Atrap-knockout blood pressure phenotype is real.
Suggested experts: Dzau VJ, Tamura K, Horiuchi M
Q: Why does human AGTRAP have 187 protein binding rows and not one of them AGTR1, when UniProt curates the interaction on the human entry with ECO:0000269, gives it a REGION 110..122 feature, and the mouse orthologue holds it as GO:0005515 IPI with P29754? The same applies to RACK1 (P63244), supported by four orthogonal assays in PMID:11733189. Checking from the partners' side rather than AGTRAP's shows the gap is symmetric, so it is not a property of this gene's record: PMID:12960423, PMID:11733189, PMID:21728994, PMID:27015675 and PMID:35414770 have produced zero GO annotations on human AGTR1, RACK1, PITPNC1, ATP2A2 and canonical PBX3 respectively. The one counter-example shows it is fixable - MGI curated PMID:35671117 reciprocally on both mouse partners (Fam114a1 IPI with Q9WVK0, Agtrap IPI with Q9D281), while neither human partner received anything.
Suggested experts: Cockcroft S, Orchard S
Q: GO:0031702 type 1 angiotensin receptor binding carries 197 annotations over 188 gene products, but only 16 entities hold it by non-IEA evidence and the experimental weight sits almost entirely in rat: ARRB2, JAK2, TYK2 and ARAP1 each by IPI. Only five human entities hold the term at all, and only AGT and BDKRB2 by IPI. Should those rat IPIs be projected to their human orthologues, and is the AT1 receptor's cytoplasmic interactome being under-annotated in human as a result?
Q: GOA imported all 188 of AGTRAP's binding rows from two-hybrid screens and none from the 23 IntAct pairs with AP-MS, co-IP or BioID support, which are almost all membrane proteins (CYB5D2, ENPP6, MBLAC2, TMEM47, TMEM106B, LAMP5, SLC15A3, SLC15A4, TMEM63A, CFTR, PAFAH2). Is that selection deliberate, and would the membrane set be a better basis for annotating a three-pass membrane protein?
Suggested experts: Orchard S, Vidal M
Q: Does human AGTRAP stimulate SERCA2a as the mouse protein does (PMID:27015675), and is the activity confined to SERCA2a or shared with SERCA1 and SERCA3? If it generalises, GO:1901896 positive regulation of ATPase-coupled calcium transmembrane transporter activity would belong on the gene alongside the molecular function proposed here.
Suggested experts: Castrop H, Kockskamper J
Q: Is the AGTRAP homodimer real? The only evidence is a single HuRI self-pair on isoform 2, which lacks residues 115-121 from within the AGTR1-interaction region - so if the protein does oligomerise, the tested species is the one least likely to be engaging the receptor.
Suggested experts: Luck K, Calderwood MA
Q: Both ISS rows proposed here (GO:0002092, GO:0141109) transfer from mouse Agtrap (Q9WVK0), which holds neither term - so as written they are ISS from an ortholog with nothing to transfer. The underlying experiments are mouse, so the annotations belong on the mouse gene first: Agtrap GO:0141109 by IDA from PubMed:27015675 (pull-down, co-immunoprecipitation, surface plasmon resonance and a calcium-uptake increase), and Agtrap GO:0002092 by IMP from PubMed:11162453 and PubMed:15757644. Would MGI make those, so the human transfers have a source?
Suggested experts: Castrop H, Kockskamper J
Q: GO:0043495 protein-membrane adaptor activity is defined as bringing a protein to a membrane 'either via membrane lipid binding or by interacting with a membrane protein', yet SUN1, SUN2 and GPR179 all hold it and are themselves integral membrane proteins that satisfy neither clause - as would AGTRAP, which anchors PITPNC1 by being the membrane protein. Should the differentia be widened to cover an integral membrane protein acting as the tether, rather than leaving each such annotation to be justified per gene?
Experiment: Co-immunoprecipitate endogenous AGTRAP and AGTR1 from human kidney proximal tubule or cardiac tissue at native expression, and submit the result to IntAct. Every existing AGTRAP-AGTR1 experiment is an over-expression assay, several of them on the mouse protein, which is the likeliest reason the interaction never entered human GOA.
Hypothesis: AGTRAP and AGTR1 associate at endogenous expression in the tissues where both are abundant, and the interaction's absence from human GOA is a curation gap rather than a biological one.
Type: Endogenous co-immunoprecipitation from human tissue
Experiment: Map the AGTRAP side of the interface by scanning alanine substitutions across residues 108-140 in a surface-AT1R internalization assay, then test whether the isoform-2 deletion (residues 115-121) is loss-of-function. That would say whether the two main isoforms differ in receptor regulation and would replace UniProt's unevidenced REGION 110..122 with mapped contacts.
Hypothesis: The AGTR1-binding determinant lies within AGTRAP residues 108-140, and isoform 2, which deletes residues 115-121 from that region, is impaired for receptor regulation.
Type: Alanine-scanning mutagenesis with a receptor internalization readout
Experiment: Repeat the SERCA2a calcium-uptake and surface plasmon resonance experiments of PMID:27015675 with human AGTRAP and human ATP2A2, including a phospholamban competition arm, to establish whether the activity is conserved and whether the two regulators share a site.
Hypothesis: Human AGTRAP stimulates human SERCA2a as the mouse proteins do, and does so by competing with phospholamban for an overlapping site.
Type: Reconstituted calcium-uptake assay and surface plasmon resonance
Experiment: Retest a stratified sample of the two-hybrid partners in a membrane-compatible assay - split-ubiquitin, NanoBiT, or proximity labelling from AGTRAP in cells - sampling separately from the topologically accessible partners and from the 28 confined to the mitochondrial matrix, the nucleoplasm or the extracellular space. The second group is a built-in negative control, and the resulting hit rate would calibrate how much of this gene's binding record to believe.
Hypothesis: The large majority of AGTRAP's two-hybrid partners do not reproduce in a membrane-compatible assay, and the reproduction rate is far lower for partners confined to compartments its cytosolic tail cannot reach.
Type: Membrane-compatible interaction assay (split-ubiquitin / NanoBiT / proximity labelling)
Experiment: Determine whether AGTRAP acts on newly synthesised or on surface AT1 receptor by pulse-chase surface biotinylation in AGTRAP-knockout versus wild-type cells. Its steady-state pool is intracellular while its measured effect is on surface receptor number, and no published experiment distinguishes retention during delivery from accelerated retrieval.
Hypothesis: AGTRAP lowers surface AT1 receptor by accelerating retrieval from the plasma membrane rather than by retaining newly synthesised receptor intracellularly.
Type: Pulse-chase cell-surface biotinylation in a knockout background
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