ANKRD44

Summary: Protein binding (IPI) with USP6 (Tre2), confirmed in the source study by pulldown, co-immunoprecipitation and colocalization with the Tre2 GAP region; modified to enzyme binding.

Reason: [PMID:16555005 "Two components of the cytoskeleton were thus identified, whose interaction with the GAP region was confirmed by GST-pulldown, co-immunoprecipitation, and colocalisation experiments."] [file:human/ANKRD44/ANKRD44-uniprot.txt "CC Q8N8A2; P35125-3: USP6; NbExp=4; IntAct=EBI-1245329, EBI-954590;"] The functional consequence for ANKRD44 is untested; enzyme binding names the partner class without overstating.

Proposed replacements: enzyme binding

Summary: Protein binding (IPI, PMID:18186651) with PPP6R1, the SAPS-domain subunit of the PP6 holoenzyme. Real and reproducible, but it is co-membership of one complex, captured here by the NEW phosphatase-complex and regulator rows; GO:0005515 adds nothing.

Reason: [PMID:18186651 "Mass spectrometry identified coprecipitating proteins as PP6 catalytic subunit and three ankyrin repeat proteins (Ankrd28, Ankrd44, and Ankrd52)."] [file:human/ANKRD44/ANKRD44-uniprot.txt "CC Q8N8A2; Q9UPN7: PPP6R1; NbExp=8; IntAct=EBI-1245329, EBI-359745;"]

Summary: Protein binding (IPI, PMID:24255178) with PPP6R1, the SAPS-domain subunit of the PP6 holoenzyme. Real and reproducible, but it is co-membership of one complex, captured here by the NEW phosphatase-complex and regulator rows; GO:0005515 adds nothing.

Reason: [PMID:18186651 "Mass spectrometry identified coprecipitating proteins as PP6 catalytic subunit and three ankyrin repeat proteins (Ankrd28, Ankrd44, and Ankrd52)."] [file:human/ANKRD44/ANKRD44-uniprot.txt "CC Q8N8A2; Q9UPN7: PPP6R1; NbExp=8; IntAct=EBI-1245329, EBI-359745;"]

Summary: Protein binding (IPI, PMID:25416956) with WWP2 from a proteome-scale yeast two-hybrid screen. There is no functional follow-up, and GO:0005515 is uninformative.

Reason: The pair is recorded as [file:human/ANKRD44/ANKRD44-uniprot.txt "CC Q8N8A2; O00308: WWP2; NbExp=5; IntAct=EBI-1245329, EBI-743923;"]

Summary: Protein binding (IPI, PMID:31515488) with WWP2 from a proteome-scale yeast two-hybrid screen. There is no functional follow-up, and GO:0005515 is uninformative.

Reason: The pair is recorded as [file:human/ANKRD44/ANKRD44-uniprot.txt "CC Q8N8A2; O00308: WWP2; NbExp=5; IntAct=EBI-1245329, EBI-743923;"]

Summary: Protein binding (IPI, PMID:32814053) with WFS1 from a proteome-scale yeast two-hybrid screen. There is no functional follow-up, and GO:0005515 is uninformative.

Reason: The pair is recorded as [file:human/ANKRD44/ANKRD44-uniprot.txt "CC Q8N8A2-2; O76024: WFS1; NbExp=3; IntAct=EBI-21636328, EBI-720609;"]

Summary: Protein binding (IPI, PMID:32814053) with HSPB1 from a proteome-scale yeast two-hybrid screen. There is no functional follow-up, and GO:0005515 is uninformative.

Reason: The pair is recorded as [file:human/ANKRD44/ANKRD44-uniprot.txt "CC Q8N8A2-2; P04792: HSPB1; NbExp=3; IntAct=EBI-21636328, EBI-352682;"]

Summary: Protein binding (IPI, PMID:32814053) with RNF11 from a proteome-scale yeast two-hybrid screen. There is no functional follow-up, and GO:0005515 is uninformative.

Reason: The pair is recorded as [file:human/ANKRD44/ANKRD44-uniprot.txt "CC Q8N8A2-2; Q9Y3C5: RNF11; NbExp=3; IntAct=EBI-21636328, EBI-396669;"]

Summary: Protein binding (IPI, PMID:33961781) with PPP6R1, the SAPS-domain subunit of the PP6 holoenzyme. Real and reproducible, but it is co-membership of one complex, captured here by the NEW phosphatase-complex and regulator rows; GO:0005515 adds nothing.

Reason: [PMID:18186651 "Mass spectrometry identified coprecipitating proteins as PP6 catalytic subunit and three ankyrin repeat proteins (Ankrd28, Ankrd44, and Ankrd52)."] [file:human/ANKRD44/ANKRD44-uniprot.txt "CC Q8N8A2; Q9UPN7: PPP6R1; NbExp=8; IntAct=EBI-1245329, EBI-359745;"]

Summary: Protein binding (IPI, PMID:35271311) with PPP6R1, the SAPS-domain subunit of the PP6 holoenzyme. Real and reproducible, but it is co-membership of one complex, captured here by the NEW phosphatase-complex and regulator rows; GO:0005515 adds nothing.

Reason: [PMID:18186651 "Mass spectrometry identified coprecipitating proteins as PP6 catalytic subunit and three ankyrin repeat proteins (Ankrd28, Ankrd44, and Ankrd52)."] [file:human/ANKRD44/ANKRD44-uniprot.txt "CC Q8N8A2; Q9UPN7: PPP6R1; NbExp=8; IntAct=EBI-1245329, EBI-359745;"]

Summary: Protein binding (IPI, PMID:40205054) with PPP6R1, the SAPS-domain subunit of the PP6 holoenzyme. Real and reproducible, but it is co-membership of one complex, captured here by the NEW phosphatase-complex and regulator rows; GO:0005515 adds nothing.

Reason: [PMID:18186651 "Mass spectrometry identified coprecipitating proteins as PP6 catalytic subunit and three ankyrin repeat proteins (Ankrd28, Ankrd44, and Ankrd52)."] [file:human/ANKRD44/ANKRD44-uniprot.txt "CC Q8N8A2; Q9UPN7: PPP6R1; NbExp=8; IntAct=EBI-1245329, EBI-359745;"]

Summary: Protein serine/threonine phosphatase complex (NEW, IDA). Endogenous ANKRD44 co-purifies with the PP6 catalytic subunit through PPP6R1 in human cells, as one of the three alternative ankyrin-repeat subunits of PP6.

Reason: [PMID:18186651 "This study used a human SAPS domain subunit FLAG-PP6R1 to identify endogenous interacting proteins."] [PMID:18186651 "Mass spectrometry identified coprecipitating proteins as PP6 catalytic subunit and three ankyrin repeat proteins (Ankrd28, Ankrd44, and Ankrd52)."] A reciprocal purification of FLAG-PPP6C from mitotic HeLa cells found the same subunits: [PMID:21187329 "This revealed that HeLa cells express all known PP6 subunits and that these are present in PPP6C complexes during mitosis"] [PMID:39014521 "PP6 functions as a heterotrimer with a catalytic subunit (PP6c), regulatory subunits (PP6R1, PP6R2, and PP6R3), and scaffold subunits (ANKRD28, ANKRD44, and ANKRD52)."] The paralog ANKRD28 carries this term by IDA (PMID:35512830); ANKRD44 is a structural subunit of the complex, so the term applies by participation.

Summary: Protein phosphatase regulator activity (NEW, IMP). Depleting ANKRD44, like ANKRD28 but unlike ANKRD52, disrupts PP6 function in mitosis.

Reason: [PMID:21187329 "Depletion of the ANKRD52 subunit had no effect on nuclear morphology, whereas ANKRD28 and ANKRD44 ankyrin repeat subunit depletion altered nuclear morphology"] [PMID:21187329 "The results presented here suggest that the ANKRD28 and 44 subunits are required for PP6 function in mitosis, whereas ANKRD52 is not."] The paralog ANKRD28 carries this term by IMP (PMID:35512830). The evidence here is phenotype-level (nuclear morphology after depletion), not a direct phosphatase activity assay with and without ANKRD44. ANKRD44 is a holoenzyme subunit, so the regulation is its own participation, not a downstream consequence.