Annexin A11 is a calcium-responsive, peripheral phospholipid-binding protein with a long low-complexity N-terminal region and a C-terminal annexin-repeat domain. It associates dynamically with nuclear, cytoplasmic and membrane compartments, forms condensates, and couples ribonucleoprotein granules to lysosomal membranes. ALG-2 and S100A6 modulate its membrane-associated condensates, and it recruits sorcin to damaged plasma membrane during repair. In dividing human cells it is a stable component of the midbody and contributes to completion of cytokinesis. Pathogenic ANXA11 variants are associated with amyotrophic lateral sclerosis and can perturb protein assembly and intracellular trafficking.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0001786 phosphatidylserine binding | IBA GO_REF:0000033 | ACCEPT | Summary: The annotated activity or location is supported as part of the core biological picture. Reason: Retain inherited phosphatidylserine binding as part of annexin membrane recognition. PTHR10502 membership is independently present; no target loss/divergence is demonstrated. Exact IBD placement remains uninspected rather than judged from donor number. Propagation Review Root cause: NO FAILURE CORE Sources checked: MGI:MGI:106008 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. PANTHER:PTN000052507 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:O76027 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P04272 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P27216 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P79134 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P81287 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. WB:WBGene00003588 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. WB:WBGene00003589 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. WB:WBGene00003590 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. dictyBase:DDB_G0269160 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. |
| GO:0003723 RNA binding | HDA PMID:22681889 The mRNA-bound proteome and its global occupancy profile on ... | ACCEPT | Summary: The annotated activity or location is supported as part of the core biological picture. Reason: RNA binding is compatible with direct newer ANXA11 biochemical evidence, not merely an inference from colocalization. Retain the HDA assertion while distinguishing the uninspected original interactome protein table from independent corroboration. PMID:42364743 explicitly reports simultaneous C-terminal RNA and lipid-vesicle binding, but its abstract-only record does not resolve construct species or full assay details; it is separate corroboration, not a reconstruction of the HDA table. Supporting Evidence: PMID:42364743 the ANXA11 Ct directly binds lipid vesicles and RNA simultaneously. |
| GO:0005509 calcium ion binding | IEA GO_REF:0000120 | ACCEPT | Summary: The annotated activity or location is supported as part of the core biological picture. Reason: Calcium binding underlies the measured calcium responsiveness of ANXA11 membrane and partner interactions. Conserved annexin domains and mouse Anxa11 donor are consistent. Exact individual InterPro rule derivations have not been reconstructed. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:P97384 UNRESOLVED Mouse Anxa11 identity and shared PTHR10502:SF29 membership were checked. The exact source annotation/transfer chain is not reconstructed. ensembl:ENSMUSP00000022416 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. InterPro:IPR001464 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. InterPro:IPR008157 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. InterPro:IPR018252 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. InterPro:IPR018502 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. InterPro:IPR037104 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. |
| GO:0005515 protein binding | IPI PMID:12445460 The penta-EF-hand domain of ALG-2 interacts with amino-termi... | MODIFY | Summary: Refine the same-paper ALG-2 interaction to its calcium-dependent activity. Reason: PMID:12445460 directly assays recombinant Anx11N interaction with ALG-2 as calcium dependent, supporting GO:0048306. Applying this refinement should merge with the already-existing GO:0048306 IPI annotation from the same paper and partner, rather than count a second functional assertion. Preserve both original audit entries and their source fields. This is a same-source specificity refinement, not an additional experiment or a NEW annotation. Proposed replacements: calcium-dependent protein binding Supporting Evidence: PMID:12445460 S-transferase (GST) fusion proteins of Anx7N and Anx11N, the direct Ca(2+)-dependent interaction was analyzed |
| GO:0005515 protein binding | IPI PMID:15197175 Annexin 11 is required for midbody formation and completion ... | KEEP AS NON CORE | Summary: Retain the verified association or specialized recruitment as a secondary aspect. Reason: The actual human A431 co-IP identifies a specific cell-cycle-dependent CHO1/KIF23 association; authors explicitly allow direct or indirect interaction. Retain the verified association as non-core and do not promote it to a demonstrated kinesin adaptor or binding interface. Supporting Evidence: PMID:15197175 annexin 11 indeed interacts (directly or indirectly) with CHO1 during cytokinesis |
| GO:0005515 protein binding | IPI PMID:18256029 Identification of Alix-type and Non-Alix-type ALG-2-binding ... | UNDECIDED | Summary: The original ANXA11–ALG-2 experiment remains unresolved at source-specific scope. Reason: The source-local ANXA11–ALG-2 pair or construct experiment has not been independently adjudicated. The separately inspected recombinant experiment in PMID:12445460 establishes calcium-dependent binding, but it does not establish what the experiment cited by this original IPI assertion measured. Withdraw the proposed calcium-dependent replacement and preserve the generic source assertion as UNDECIDED. This neither denies the interaction nor concludes that the original paper lacked a calcium-dependence assay. The independently supported calcium-dependent function is already retained in its own source-backed annotations. |
| GO:0005515 protein binding | IPI PMID:25910212 Widespread macromolecular interaction perturbations in human... | UNDECIDED | Summary: The original ANXA11–ALG-2 experiment remains unresolved at source-specific scope. Reason: The source-local ANXA11–ALG-2 pair or construct experiment has not been independently adjudicated. The separately inspected recombinant experiment in PMID:12445460 establishes calcium-dependent binding, but it does not establish what the experiment cited by this original IPI assertion measured. Withdraw the proposed calcium-dependent replacement and preserve the generic source assertion as UNDECIDED. This neither denies the interaction nor concludes that the original paper lacked a calcium-dependence assay. The independently supported calcium-dependent function is already retained in its own source-backed annotations. |
| GO:0005515 protein binding | IPI PMID:25910212 Widespread macromolecular interaction perturbations in human... | UNDECIDED | Summary: The curated interaction remains unresolved at the pair-specific experiment level. Reason: The relevant ANXA11 pair/construct experiment or supplementary table has not been independently inspected. A cached main body or full-text availability flag does not close that evidence gap, and no specific replacement function is established for this pair. Retain UNDECIDED under the experimental-evidence access rule; genericity, omission from narrative text and different usual compartments do not establish that the interaction is false. |
| GO:0005515 protein binding | IPI PMID:25910212 Widespread macromolecular interaction perturbations in human... | UNDECIDED | Summary: The curated interaction remains unresolved at the pair-specific experiment level. Reason: The relevant ANXA11 pair/construct experiment or supplementary table has not been independently inspected. A cached main body or full-text availability flag does not close that evidence gap, and no specific replacement function is established for this pair. Retain UNDECIDED under the experimental-evidence access rule; genericity, omission from narrative text and different usual compartments do not establish that the interaction is false. |
| GO:0005515 protein binding | IPI PMID:31515488 Extensive disruption of protein interactions by genetic vari... | UNDECIDED | Summary: The original ANXA11–ALG-2 experiment remains unresolved at source-specific scope. Reason: The source-local ANXA11–ALG-2 pair or construct experiment has not been independently adjudicated. The separately inspected recombinant experiment in PMID:12445460 establishes calcium-dependent binding, but it does not establish what the experiment cited by this original IPI assertion measured. Withdraw the proposed calcium-dependent replacement and preserve the generic source assertion as UNDECIDED. This neither denies the interaction nor concludes that the original paper lacked a calcium-dependence assay. The independently supported calcium-dependent function is already retained in its own source-backed annotations. |
| GO:0005515 protein binding | IPI PMID:31515488 Extensive disruption of protein interactions by genetic vari... | UNDECIDED | Summary: The curated interaction remains unresolved at the pair-specific experiment level. Reason: The relevant ANXA11 pair/construct experiment or supplementary table has not been independently inspected. A cached main body or full-text availability flag does not close that evidence gap, and no specific replacement function is established for this pair. Retain UNDECIDED under the experimental-evidence access rule; genericity, omission from narrative text and different usual compartments do not establish that the interaction is false. |
| GO:0005515 protein binding | IPI PMID:31515488 Extensive disruption of protein interactions by genetic vari... | UNDECIDED | Summary: The curated interaction remains unresolved at the pair-specific experiment level. Reason: The relevant ANXA11 pair/construct experiment or supplementary table has not been independently inspected. A cached main body or full-text availability flag does not close that evidence gap, and no specific replacement function is established for this pair. Retain UNDECIDED under the experimental-evidence access rule; genericity, omission from narrative text and different usual compartments do not establish that the interaction is false. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The curated interaction remains unresolved at the pair-specific experiment level. Reason: The relevant ANXA11 pair/construct experiment or supplementary table has not been independently inspected. A cached main body or full-text availability flag does not close that evidence gap, and no specific replacement function is established for this pair. Retain UNDECIDED under the experimental-evidence access rule; genericity, omission from narrative text and different usual compartments do not establish that the interaction is false. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The curated interaction remains unresolved at the pair-specific experiment level. Reason: The relevant ANXA11 pair/construct experiment or supplementary table has not been independently inspected. A cached main body or full-text availability flag does not close that evidence gap, and no specific replacement function is established for this pair. Retain UNDECIDED under the experimental-evidence access rule; genericity, omission from narrative text and different usual compartments do not establish that the interaction is false. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The curated interaction remains unresolved at the pair-specific experiment level. Reason: The relevant ANXA11 pair/construct experiment or supplementary table has not been independently inspected. A cached main body or full-text availability flag does not close that evidence gap, and no specific replacement function is established for this pair. Retain UNDECIDED under the experimental-evidence access rule; genericity, omission from narrative text and different usual compartments do not establish that the interaction is false. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The curated interaction remains unresolved at the pair-specific experiment level. Reason: The relevant ANXA11 pair/construct experiment or supplementary table has not been independently inspected. A cached main body or full-text availability flag does not close that evidence gap, and no specific replacement function is established for this pair. Retain UNDECIDED under the experimental-evidence access rule; genericity, omission from narrative text and different usual compartments do not establish that the interaction is false. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The curated interaction remains unresolved at the pair-specific experiment level. Reason: The relevant ANXA11 pair/construct experiment or supplementary table has not been independently inspected. A cached main body or full-text availability flag does not close that evidence gap, and no specific replacement function is established for this pair. Retain UNDECIDED under the experimental-evidence access rule; genericity, omission from narrative text and different usual compartments do not establish that the interaction is false. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The curated interaction remains unresolved at the pair-specific experiment level. Reason: The relevant ANXA11 pair/construct experiment or supplementary table has not been independently inspected. A cached main body or full-text availability flag does not close that evidence gap, and no specific replacement function is established for this pair. Retain UNDECIDED under the experimental-evidence access rule; genericity, omission from narrative text and different usual compartments do not establish that the interaction is false. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The curated interaction remains unresolved at the pair-specific experiment level. Reason: The relevant ANXA11 pair/construct experiment or supplementary table has not been independently inspected. A cached main body or full-text availability flag does not close that evidence gap, and no specific replacement function is established for this pair. Retain UNDECIDED under the experimental-evidence access rule; genericity, omission from narrative text and different usual compartments do not establish that the interaction is false. |
| GO:0005515 protein binding | IPI PMID:9268363 Calcium-dependent binding of sorcin to the N-terminal domain... | MODIFY | Summary: A calcium-dependent binding term is more informative for this experimentally corroborated partner. Reason: Original paper Methods explicitly clone human ANXA11 aa1-121; Results and Fig6 demonstrate recombinant sorcin binding with calcium-dependent GST controls. Refine the generic pair to calcium-dependent protein binding. Do not transfer the separate synexin granule-aggregation results to ANXA11. The targeted original-paper Methods/Results/Figure 6 read and accessible article URL are recorded in the notes; the unchanged normal cache is abstract-only and describes synexin. Independent PMID:42574611 Figure 8 also demonstrates calcium-dependent recruitment of purified sorcin to liposomes by ANXA11; the purchased ANXA11 reagent species is not specified in the inspected Methods, whereas the original human fragment is identified. Proposed replacements: calcium-dependent protein binding Supporting Evidence: PMID:42574611 sorcin binds directly and specifically to ANXA11 and that ANXA11 is sufficient to recruit sorcin to membranes in the presence of Ca2+ |
| GO:0005544 calcium-dependent phospholipid binding | IBA GO_REF:0000033 | ACCEPT | Summary: The annotated activity or location is supported as part of the core biological picture. Reason: Retain calcium-dependent phospholipid binding as core molecular activity. Direct ANXA11 membrane reconstitution supports the biological claim; inherited/domain inference details are separately bounded. Propagation Review Root cause: NO FAILURE CORE Sources checked: FB:FBgn0000084 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. MGI:MGI:108481 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. PANTHER:PTN000052507 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. RGD:2119 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. RGD:2120 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. RGD:621172 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. RGD:621173 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P04083 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P07355 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P08133 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P08758 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P09525 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P12429 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P13928 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P19619 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P27216 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P79134 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. WB:WBGene00003588 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. dictyBase:DDB_G0269160 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. Supporting Evidence: PMID:40118863 ANXA11 binds to PI(3)P-containing GUV membranes in a Ca2+-dependent manner PMID:40118863 Constructs encoding ANXA11 FL (aa 1-505), ANXA11 LCD (aa 1-185), ANXA11 ARD (aa 186-505), ALG2, CALC and G3BP1 were cloned by PCR amplification from HeLa cell cDNA |
| GO:0005544 calcium-dependent phospholipid binding | IEA GO_REF:0000120 | ACCEPT | Summary: The annotated activity or location is supported as part of the core biological picture. Reason: Retain calcium-dependent phospholipid binding as core molecular activity. Direct ANXA11 membrane reconstitution supports the biological claim; inherited/domain inference details are separately bounded. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:P97384 UNRESOLVED Mouse Anxa11 identity and shared PTHR10502:SF29 membership were checked. The exact source annotation/transfer chain is not reconstructed. ensembl:ENSMUSP00000022416 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. InterPro:IPR001464 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. InterPro:IPR008157 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. InterPro:IPR018252 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. InterPro:IPR018502 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. InterPro:IPR037104 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. Supporting Evidence: PMID:40118863 ANXA11 binds to PI(3)P-containing GUV membranes in a Ca2+-dependent manner PMID:40118863 Constructs encoding ANXA11 FL (aa 1-505), ANXA11 LCD (aa 1-185), ANXA11 ARD (aa 186-505), ALG2, CALC and G3BP1 were cloned by PCR amplification from HeLa cell cDNA |
| GO:0005634 nucleus | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: Retain the observed nuclear pool as a secondary localization. Reason: Human ANXA11 imaging and HPA support nuclear or nucleoplasmic localization, including regulated redistribution during the cell cycle. Retain this positive location as a secondary pool; the examined evidence does not assign a distinct molecular activity to nuclear ANXA11. This classification does not dispute the localization or infer loss of function. Independent target observations do not reconstruct every source-local experiment or ancestral placement. Target self-inclusion is legitimate descendant experimental evidence, not circularity; the exact PAINT node placement remains uninspected. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: MGI:MGI:88031 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. MGI:MGI:88246 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. PANTHER:PTN000052508 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. RGD:2118 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P04083 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P04272 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P07355 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P09525 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P13214 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P20073 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P46193 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P50995 UNRESOLVED Target self-inclusion is valid descendant experimental evidence, not circularity. The exact ancestral placement remains uninspected. dictyBase:DDB_G0269160 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. Supporting Evidence: PMID:15197175 Both annexin 11 and CHO1 are located mainly inside the nucleus during interphase and are excluded from the nucleoli. |
| GO:0005635 nuclear envelope | IDA PMID:12601007 Calcium- and cell cycle-dependent association of annexin 11 ... | ACCEPT | Summary: The annotated activity or location is supported as part of the core biological picture. Reason: Calcium- and cell-cycle-dependent nuclear-envelope association is directly observed in human A431 cells in PMID:12601007 and corroborated by the human midbody study PMID:15197175. Supporting Evidence: PMID:12601007 elevation of intracellular Ca2+ leads to translocation of annexin 11 from the nucleus to the nuclear envelope |
| GO:0005635 nuclear envelope | IDA PMID:15197175 Annexin 11 is required for midbody formation and completion ... | ACCEPT | Summary: The annotated activity or location is supported as part of the core biological picture. Reason: Calcium- and cell-cycle-dependent nuclear-envelope association is directly observed in human A431 cells in PMID:12601007 and corroborated by the human midbody study PMID:15197175. Supporting Evidence: PMID:12601007 elevation of intracellular Ca2+ leads to translocation of annexin 11 from the nucleus to the nuclear envelope |
| GO:0005635 nuclear envelope | IEA GO_REF:0000044 | ACCEPT | Summary: The annotated activity or location is supported as part of the core biological picture. Reason: Calcium- and cell-cycle-dependent nuclear-envelope association is directly observed in human A431 cells in PMID:12601007 and corroborated by the human midbody study PMID:15197175. The exact UniProt vocabulary mapping is retained separately from the independent experimental corroboration. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB-SubCell:SL-0178 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. Supporting Evidence: PMID:12601007 elevation of intracellular Ca2+ leads to translocation of annexin 11 from the nucleus to the nuclear envelope |
| GO:0005635 nuclear envelope | NAS PMID:12577318 Calcyclin (S100A6) regulates pulmonary fibroblast proliferat... | ACCEPT | Summary: The annotated activity or location is supported as part of the core biological picture. Reason: Calcium- and cell-cycle-dependent nuclear-envelope association is directly observed in human A431 cells in PMID:12601007 and corroborated by the human midbody study PMID:15197175. The original PMID:12577318 abstract does not resolve its ANXA11 experiment; acceptance rests on this independent target-specific location evidence, without transferring S100A6 perturbation effects. Supporting Evidence: PMID:12601007 elevation of intracellular Ca2+ leads to translocation of annexin 11 from the nucleus to the nuclear envelope |
| GO:0005654 nucleoplasm | IDA GO_REF:0000052 | KEEP AS NON CORE | Summary: Retain the observed nuclear pool as a secondary localization. Reason: Human ANXA11 imaging and HPA support nuclear or nucleoplasmic localization, including regulated redistribution during the cell cycle. Retain this positive location as a secondary pool; the examined evidence does not assign a distinct molecular activity to nuclear ANXA11. This classification does not dispute the localization or infer loss of function. Independent target observations do not reconstruct every source-local experiment or ancestral placement. Supporting Evidence: PMID:15197175 Both annexin 11 and CHO1 are located mainly inside the nucleus during interphase and are excluded from the nucleoli. HPA:ENSG00000122359 Nucleoplasm Cytosol |
| GO:0005654 nucleoplasm | IDA PMID:12601007 Calcium- and cell cycle-dependent association of annexin 11 ... | KEEP AS NON CORE | Summary: Retain the observed nuclear pool as a secondary localization. Reason: Human ANXA11 imaging and HPA support nuclear or nucleoplasmic localization, including regulated redistribution during the cell cycle. Retain this positive location as a secondary pool; the examined evidence does not assign a distinct molecular activity to nuclear ANXA11. This classification does not dispute the localization or infer loss of function. Independent target observations do not reconstruct every source-local experiment or ancestral placement. Supporting Evidence: PMID:15197175 Both annexin 11 and CHO1 are located mainly inside the nucleus during interphase and are excluded from the nucleoli. |
| GO:0005654 nucleoplasm | IDA PMID:15197175 Annexin 11 is required for midbody formation and completion ... | KEEP AS NON CORE | Summary: Retain the observed nuclear pool as a secondary localization. Reason: Human ANXA11 imaging and HPA support nuclear or nucleoplasmic localization, including regulated redistribution during the cell cycle. Retain this positive location as a secondary pool; the examined evidence does not assign a distinct molecular activity to nuclear ANXA11. This classification does not dispute the localization or infer loss of function. Independent target observations do not reconstruct every source-local experiment or ancestral placement. Supporting Evidence: PMID:15197175 Both annexin 11 and CHO1 are located mainly inside the nucleus during interphase and are excluded from the nucleoli. |
| GO:0005654 nucleoplasm | IDA PMID:7508441 The 56K autoantigen is identical to human annexin XI. | KEEP AS NON CORE | Summary: Retain the observed nuclear pool as a secondary localization. Reason: Human ANXA11 imaging and HPA support nuclear or nucleoplasmic localization, including regulated redistribution during the cell cycle. Retain this positive location as a secondary pool; the examined evidence does not assign a distinct molecular activity to nuclear ANXA11. This classification does not dispute the localization or infer loss of function. Independent target observations do not reconstruct every source-local experiment or ancestral placement. Supporting Evidence: PMID:15197175 Both annexin 11 and CHO1 are located mainly inside the nucleus during interphase and are excluded from the nucleoli. |
| GO:0005654 nucleoplasm | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: Retain the observed nuclear pool as a secondary localization. Reason: Human ANXA11 imaging and HPA support nuclear or nucleoplasmic localization, including regulated redistribution during the cell cycle. Retain this positive location as a secondary pool; the examined evidence does not assign a distinct molecular activity to nuclear ANXA11. This classification does not dispute the localization or infer loss of function. Independent target observations do not reconstruct every source-local experiment or ancestral placement. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: UniProtKB-SubCell:SL-0190 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. Supporting Evidence: PMID:15197175 Both annexin 11 and CHO1 are located mainly inside the nucleus during interphase and are excluded from the nucleoli. |
| GO:0005654 nucleoplasm | NAS PMID:12577318 Calcyclin (S100A6) regulates pulmonary fibroblast proliferat... | KEEP AS NON CORE | Summary: Retain the observed nuclear pool as a secondary localization. Reason: Human ANXA11 imaging and HPA support nuclear or nucleoplasmic localization, including regulated redistribution during the cell cycle. Retain this positive location as a secondary pool; the examined evidence does not assign a distinct molecular activity to nuclear ANXA11. This classification does not dispute the localization or infer loss of function. Independent target observations do not reconstruct every source-local experiment or ancestral placement. Supporting Evidence: PMID:15197175 Both annexin 11 and CHO1 are located mainly inside the nucleus during interphase and are excluded from the nucleoli. |
| GO:0005737 cytoplasm | EXP PMID:28469040 Mutations in the vesicular trafficking protein annexin A11 a... | ACCEPT | Summary: The annotated activity or location is supported as part of the core biological picture. Reason: Independent human ANXA11-specific immunofluorescence and human cell-cycle experiments corroborate a cytoplasmic pool with regulated nuclear/membrane redistribution. The original wild-type controls were also read; disease-mutant aggregates do not alone define normal localization. Supporting Evidence: PMID:7508441 Anti-56K autoantibody exhibits both a cytoplasmic and a nuclear staining in immunofluorescence experiments. |
| GO:0005737 cytoplasm | IBA GO_REF:0000033 | ACCEPT | Summary: The annotated activity or location is supported as part of the core biological picture. Reason: Independent human ANXA11-specific immunofluorescence and human cell-cycle experiments corroborate a cytoplasmic pool with regulated nuclear/membrane redistribution. Propagation Review Root cause: NO FAILURE CORE Sources checked: AGI_LocusCode:AT1G35720 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. FB:FBgn0000083 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. MGI:MGI:108481 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. MGI:MGI:1917037 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. MGI:MGI:88031 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. MGI:MGI:88246 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. MGI:MGI:88255 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. MGI:MGI:96819 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. PANTHER:PTN000052507 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. RGD:2118 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. RGD:2120 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. RGD:621170 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. RGD:621173 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:O76027 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P04083 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P04272 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P07355 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P08133 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P09525 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P12429 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P13214 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P13928 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P19619 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P20072 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P20073 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P46193 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P50994 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P50995 UNRESOLVED Target self-inclusion is valid descendant experimental evidence, not circularity. The exact ancestral placement remains uninspected. UniProtKB:P79134 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:Q29471 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:Q6TEQ7 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. ZFIN:ZDB-GENE-030131-9076 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. dictyBase:DDB_G0269160 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. dictyBase:DDB_G0284261 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. Supporting Evidence: PMID:7508441 Anti-56K autoantibody exhibits both a cytoplasmic and a nuclear staining in immunofluorescence experiments. |
| GO:0005737 cytoplasm | IDA PMID:12601007 Calcium- and cell cycle-dependent association of annexin 11 ... | ACCEPT | Summary: The annotated activity or location is supported as part of the core biological picture. Reason: Independent human ANXA11-specific immunofluorescence and human cell-cycle experiments corroborate a cytoplasmic pool with regulated nuclear/membrane redistribution. Supporting Evidence: PMID:7508441 Anti-56K autoantibody exhibits both a cytoplasmic and a nuclear staining in immunofluorescence experiments. |
| GO:0005737 cytoplasm | IDA PMID:12805373 Dissecting the cellular functions of annexin XI using recomb... | ACCEPT | Summary: The annotated activity or location is supported as part of the core biological picture. Reason: Independent human ANXA11-specific immunofluorescence and human cell-cycle experiments corroborate a cytoplasmic pool with regulated nuclear/membrane redistribution. The original PMID:12805373 abstract explicitly includes COS-7 cells; host species and recombinant protein identity are not conflated. Supporting Evidence: PMID:7508441 Anti-56K autoantibody exhibits both a cytoplasmic and a nuclear staining in immunofluorescence experiments. |
| GO:0005737 cytoplasm | IDA PMID:7508441 The 56K autoantigen is identical to human annexin XI. | ACCEPT | Summary: The annotated activity or location is supported as part of the core biological picture. Reason: Independent human ANXA11-specific immunofluorescence and human cell-cycle experiments corroborate a cytoplasmic pool with regulated nuclear/membrane redistribution. Supporting Evidence: PMID:7508441 Anti-56K autoantibody exhibits both a cytoplasmic and a nuclear staining in immunofluorescence experiments. |
| GO:0005737 cytoplasm | IEA GO_REF:0000120 | ACCEPT | Summary: The annotated activity or location is supported as part of the core biological picture. Reason: Independent human ANXA11-specific immunofluorescence and human cell-cycle experiments corroborate a cytoplasmic pool with regulated nuclear/membrane redistribution. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:P97384 UNRESOLVED Mouse Anxa11 identity and shared PTHR10502:SF29 membership were checked. The exact source annotation/transfer chain is not reconstructed. ensembl:ENSMUSP00000022416 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB-SubCell:SL-0086 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. Supporting Evidence: PMID:7508441 Anti-56K autoantibody exhibits both a cytoplasmic and a nuclear staining in immunofluorescence experiments. |
| GO:0005819 spindle | IDA PMID:12805373 Dissecting the cellular functions of annexin XI using recomb... | KEEP AS NON CORE | Summary: Retain cell-cycle-dependent spindle localization as a secondary pool. Reason: Actual human A431 imaging shows spindle-pole and spindle-midzone recruitment. Retain the observed localization as non-core without assigning tubulin binding from a co-IP whose control also contained tubulin. The original PMID:12805373 monkey-host observation remains distinct from independent human corroboration. This observed pool is separate from the stable midbody matrix role represented below; no incorrect-location or lost-function claim is made. Supporting Evidence: PMID:15197175 11 translocates from the nucleus to the spindle poles in metaphase and to the spindle midzone in anaphase. |
| GO:0005819 spindle | IDA PMID:15197175 Annexin 11 is required for midbody formation and completion ... | KEEP AS NON CORE | Summary: Retain cell-cycle-dependent spindle localization as a secondary pool. Reason: Actual human A431 imaging shows spindle-pole and spindle-midzone recruitment. Retain the observed localization as non-core without assigning tubulin binding from a co-IP whose control also contained tubulin. The original PMID:12805373 monkey-host observation remains distinct from independent human corroboration. This observed pool is separate from the stable midbody matrix role represented below; no incorrect-location or lost-function claim is made. Supporting Evidence: PMID:15197175 11 translocates from the nucleus to the spindle poles in metaphase and to the spindle midzone in anaphase. |
| GO:0005819 spindle | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: Retain cell-cycle-dependent spindle localization as a secondary pool. Reason: Actual human A431 imaging shows spindle-pole and spindle-midzone recruitment. Retain the observed localization as non-core without assigning tubulin binding from a co-IP whose control also contained tubulin. The original PMID:12805373 monkey-host observation remains distinct from independent human corroboration. This observed pool is separate from the stable midbody matrix role represented below; no incorrect-location or lost-function claim is made. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: UniProtKB-SubCell:SL-0251 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. Supporting Evidence: PMID:15197175 11 translocates from the nucleus to the spindle poles in metaphase and to the spindle midzone in anaphase. |
| GO:0005829 cytosol | IDA GO_REF:0000052 | ACCEPT | Summary: The annotated activity or location is supported as part of the core biological picture. Reason: Retain supported cytosolic pool as part of normal dynamic distribution. Supporting Evidence: HPA:ENSG00000122359 Nucleoplasm Cytosol |
| GO:0005886 plasma membrane | IBA GO_REF:0000033 | ACCEPT | Summary: The annotated activity or location is supported as part of the core biological picture. Reason: Retain plasma membrane localization as a calcium/damage-associated pool, independently supported by endogenous ANXA11 staining at lesions in human U2OS. This is not constitutive integral membrane residency and not a reconstructed donor node. Propagation Review Root cause: NO FAILURE CORE Sources checked: MGI:MGI:106008 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. MGI:MGI:1917037 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. MGI:MGI:1923711 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. MGI:MGI:88031 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. MGI:MGI:88246 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. MGI:MGI:96819 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. PANTHER:PTN000052507 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. RGD:2118 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. RGD:2120 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. RGD:621170 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. RGD:621172 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. RGD:621173 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P04083 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P07355 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P09525 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P12429 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P13928 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P17785 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P19619 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P27216 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P79134 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:Q29471 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. WB:WBGene00003588 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. ZFIN:ZDB-GENE-041221-2 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. dictyBase:DDB_G0269160 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. dictyBase:DDB_G0284261 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. Supporting Evidence: PMID:9188810 annexin XI translocated to specific granules and secretory vesicles/plasma membrane at 100 micromol/l calcium. PMID:42574611 ANXA11 and sorcin are simultaneously recruited to PM lesions, followed sequentially by ALIX and then CHMP4B. |
| GO:0006909 phagocytosis | IBA GO_REF:0000033 | UNDECIDED | Summary: The exact source-specific assertion remains unresolved. Reason: The directly accessible ANXA11 evidence establishes recruitment to neutrophil granules/phagosomes, but the original full process experiment and exact PAINT ancestral placement have not been inspected. Recruitment alone does not settle a mechanistic contribution to engulfment. Preserve the assertion as uncertain without treating the self-donor as circular or denying the curated experiment. Propagation Review Root cause: UNRESOLVED Sources checked: PANTHER:PTN002574813 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P50995 UNRESOLVED Target self-inclusion is valid descendant experimental evidence, not circularity. The exact ancestral placement remains uninspected. Supporting Evidence: PMID:9188810 Periphagosomal translocation of annexin XI occurred during phagocytosis of yeast particles |
| GO:0006909 phagocytosis | IEP PMID:9188810 Translocation of annexin XI to neutrophil subcellular organe... | UNDECIDED | Summary: The exact source-specific assertion remains unresolved. Reason: The directly accessible ANXA11 evidence establishes recruitment to neutrophil granules/phagosomes, but the original full process experiment and exact PAINT ancestral placement have not been inspected. Recruitment alone does not settle a mechanistic contribution to engulfment. Preserve the assertion as uncertain without treating the self-donor as circular or denying the curated experiment. Supporting Evidence: PMID:9188810 Periphagosomal translocation of annexin XI occurred during phagocytosis of yeast particles |
| GO:0008429 phosphatidylethanolamine binding | IEA GO_REF:0000107 | UNDECIDED | Summary: The exact source-specific assertion remains unresolved. Reason: The mouse Anxa11 donor is correctly identified, and primary mouse lipid work is biologically compatible with PE binding. Exact electronic transfer support and assay specificity have not yet been closed; broad membrane recognition is secure without promoting this particular lipid specificity. Propagation Review Root cause: UNRESOLVED Sources checked: UniProtKB:P97384 UNRESOLVED Mouse Anxa11 identity and shared PTHR10502:SF29 membership were checked. The exact source annotation/transfer chain is not reconstructed. ensembl:ENSMUSP00000022416 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. |
| GO:0012506 vesicle membrane | IBA GO_REF:0000033 | ACCEPT | Summary: The annotated activity or location is supported as part of the core biological picture. Reason: Retain vesicle-membrane association as core membrane engagement, corroborated independently by lysosomal membrane tethering. Preserve its distinct inherited PAINT assertion without claiming the original node was reconstructed. PMID:31539493 examines lysosome-associated ANXA11 in cells; PMID:40118863 independently reconstitutes RNP coupling to lysosome-like phospholipid vesicles using HeLa-derived ANXA11. The reconstituted GUV is a membrane model, not an endogenous organelle. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000052508 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. RGD:2118 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. RGD:2120 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P07355 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P08133 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P09525 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P12429 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P13214 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P13928 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P19619 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P20072 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P20073 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P50994 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P79134 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. dictyBase:DDB_G0269160 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. Supporting Evidence: PMID:40118863 Co-incubation of the FAPS-purified RNP granules with ANXA11-coated GUVs results in accumulation of the RNP granules onto the GUV surface PMID:31539493 addition of both ANXA11 and calcium promoted contact between G3BP1-positive RNA granules and liposomes |
| GO:0016020 membrane | HDA PMID:19946888 Defining the membrane proteome of NK cells. | ACCEPT | Summary: Independent human-cell recruitment supports broad membrane association. Reason: Actual PMID:42574611 human U2OS injury experiments show endogenous ANXA11 recruitment to plasma-membrane lesions, independently supporting broad membrane association. Replace the in-vitro granule-on-GUV attachment with this cellular target observation. The original NK-cell proteomics peptide/table entry remains uninspected; independent corroboration does not reconstruct that source-specific detection. No integral transmembrane segment or specialized extracellular location is inferred. Supporting Evidence: PMID:42574611 ANXA11 and sorcin are simultaneously recruited to PM lesions, followed sequentially by ALIX and then CHMP4B. |
| GO:0023026 MHC class II protein complex binding | HDA PMID:20458337 MHC class II-associated proteins in B-cell exosomes and pote... | UNDECIDED | Summary: The exact source-specific assertion remains unresolved. Reason: The B-cell exosome/MHC-II-associated proteome does not, in its accessible abstract, identify the ANXA11 pair experiment. Exact complex/binary association and its relevance remain unresolved; do not infer wrong gene or direct binding from a generic list. |
| GO:0030496 midbody | IDA PMID:15197175 Annexin 11 is required for midbody formation and completion ... | ACCEPT | Summary: The annotated activity or location is supported as part of the core biological picture. Reason: ANXA11 is an endogenous stable midbody component in multiple human lines. This location is coupled to a direct structural requirement for terminal cytokinesis. Supporting Evidence: PMID:15197175 Annexin 11 is recruited to the midbody in late telophase, where it forms part of the detergent-resistant matrix |
| GO:0031012 extracellular matrix | HDA PMID:25037231 Extracellular matrix signatures of human primary metastatic ... | UNDECIDED | Summary: The exact source-specific assertion remains unresolved. Reason: The accessible source context does not expose the ANXA11-specific proteomic table or peptide evidence for this specialized compartment. Broad annexin membrane binding is not independent confirmation of this location. Retain uncertainty pending target-specific evidence, without inferring contamination, wrong-gene attribution or contradiction. |
| GO:0031012 extracellular matrix | HDA PMID:28344315 Proteomic characterization of human multiple myeloma bone ma... | UNDECIDED | Summary: The exact source-specific assertion remains unresolved. Reason: The accessible source context does not expose the ANXA11-specific proteomic table or peptide evidence for this specialized compartment. Broad annexin membrane binding is not independent confirmation of this location. Retain uncertainty pending target-specific evidence, without inferring contamination, wrong-gene attribution or contradiction. |
| GO:0032506 cytokinetic process | IBA GO_REF:0000033 | ACCEPT | Summary: The annotated activity or location is supported as part of the core biological picture. Reason: Endogenous ANXA11 is a stable midbody constituent in human cells; depletion disrupts midbody matrix formation and prevents terminal cell separation. Structural participation is supported beyond a secondary viability phenotype. This retains an existing process assertion. The target self-donor is valid descendant evidence; exact PAINT node placement remains uninspected. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN002574813 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. UniProtKB:P50995 UNRESOLVED Target self-inclusion is valid descendant experimental evidence, not circularity. The exact ancestral placement remains uninspected. Supporting Evidence: PMID:15197175 Annexin 11 is recruited to the midbody in late telophase, where it forms part of the detergent-resistant matrix PMID:15197175 cells lacking annexin 11 fail to establish a functional midbody. |
| GO:0032506 cytokinetic process | IMP PMID:15197175 Annexin 11 is required for midbody formation and completion ... | ACCEPT | Summary: The annotated activity or location is supported as part of the core biological picture. Reason: Endogenous ANXA11 is a stable midbody constituent in human cells; depletion disrupts midbody matrix formation and prevents terminal cell separation. Structural participation is supported beyond a secondary viability phenotype. This retains an existing process assertion. Supporting Evidence: PMID:15197175 Annexin 11 is recruited to the midbody in late telophase, where it forms part of the detergent-resistant matrix PMID:15197175 cells lacking annexin 11 fail to establish a functional midbody. |
| GO:0042470 melanosome | IEA GO_REF:0000044 | UNDECIDED | Summary: The exact source-specific assertion remains unresolved. Reason: The accessible source context does not expose the ANXA11-specific proteomic table or peptide evidence for this specialized compartment. Broad annexin membrane binding is not independent confirmation of this location. Retain uncertainty pending target-specific evidence, without inferring contamination, wrong-gene attribution or contradiction. Propagation Review Root cause: UNRESOLVED Sources checked: UniProtKB-SubCell:SL-0161 UNRESOLVED The exact propagation rule or ancestral placement was not reconstructed; independent target evidence is assessed separately. |
| GO:0042581 specific granule | IDA PMID:9188810 Translocation of annexin XI to neutrophil subcellular organe... | KEEP AS NON CORE | Summary: Retain the verified association or specialized recruitment as a secondary aspect. Reason: Retain experimentally described neutrophil granule/phagosomal recruitment as specialized non-core locations. Specific and azurophil granules have different tested calcium thresholds; do not generalize to constitutive intraluminal residence. Supporting Evidence: PMID:9188810 annexin XI translocated to specific granules and secretory vesicles/plasma membrane at 100 micromol/l calcium. |
| GO:0042582 azurophil granule | IDA PMID:9188810 Translocation of annexin XI to neutrophil subcellular organe... | KEEP AS NON CORE | Summary: Retain the verified association or specialized recruitment as a secondary aspect. Reason: Retain experimentally described neutrophil granule/phagosomal recruitment as specialized non-core locations. Specific and azurophil granules have different tested calcium thresholds; do not generalize to constitutive intraluminal residence. Supporting Evidence: PMID:9188810 When raising the concentration of calcium to 1 mmol/l, annexin XI translocated to the azurophil granules as well. |
| GO:0044548 S100 protein binding | IPI PMID:12577318 Calcyclin (S100A6) regulates pulmonary fibroblast proliferat... | ACCEPT | Summary: The annotated activity or location is supported as part of the core biological picture. Reason: S100-family partner binding is a recurring biochemical feature, corroborated specifically for S100A6 by the actual Panc-1 protein identification, ANXA11 mutant/WT co-IP and recent CALC modulation of ANXA11 condensation. Do not borrow ANXA2 motility experiments. Supporting Evidence: PMID:19724273 Our proteomic screen to identify S100A6 interacting proteins revealed annexin 11, annexin 2, tropomyosin beta |
| GO:0044548 S100 protein binding | IPI PMID:19724273 S100A6 binds to annexin 2 in pancreatic cancer cells and pro... | ACCEPT | Summary: The annotated activity or location is supported as part of the core biological picture. Reason: S100-family partner binding is a recurring biochemical feature, corroborated specifically for S100A6 by the actual Panc-1 protein identification, ANXA11 mutant/WT co-IP and recent CALC modulation of ANXA11 condensation. Do not borrow ANXA2 motility experiments. Supporting Evidence: PMID:19724273 Our proteomic screen to identify S100A6 interacting proteins revealed annexin 11, annexin 2, tropomyosin beta |
| GO:0045335 phagocytic vesicle | IDA PMID:9188810 Translocation of annexin XI to neutrophil subcellular organe... | KEEP AS NON CORE | Summary: Retain the verified association or specialized recruitment as a secondary aspect. Reason: Retain experimentally described neutrophil granule/phagosomal recruitment as specialized non-core locations. Specific and azurophil granules have different tested calcium thresholds; do not generalize to constitutive intraluminal residence. Supporting Evidence: PMID:9188810 Periphagosomal translocation of annexin XI occurred during phagocytosis of yeast particles |
| GO:0048306 calcium-dependent protein binding | IPI PMID:11883939 ALG-2 interacts with the amino-terminal domain of annexin XI... | ACCEPT | Summary: The annotated activity or location is supported as part of the core biological picture. Reason: Retain explicitly demonstrated calcium-dependent ALG-2 protein binding as a functional regulator interaction. Supporting Evidence: PMID:12445460 S-transferase (GST) fusion proteins of Anx7N and Anx11N, the direct Ca(2+)-dependent interaction was analyzed |
| GO:0048306 calcium-dependent protein binding | IPI PMID:11883939 ALG-2 interacts with the amino-terminal domain of annexin XI... | UNDECIDED | Summary: The exact source-specific assertion remains unresolved. Reason: The accessible source establishes ANXA11–ALG-2 binding and describes peflin elsewhere, but does not resolve this exact ANXA11–PEF1 assertion. An ALG-2–peflin heterodimer alone would not prove direct peflin contact. Keep uncertain pending original pair experiment. |
| GO:0048306 calcium-dependent protein binding | IPI PMID:12445460 The penta-EF-hand domain of ALG-2 interacts with amino-termi... | ACCEPT | Summary: The annotated activity or location is supported as part of the core biological picture. Reason: Retain explicitly demonstrated calcium-dependent ALG-2 protein binding as a functional regulator interaction. Supporting Evidence: PMID:12445460 S-transferase (GST) fusion proteins of Anx7N and Anx11N, the direct Ca(2+)-dependent interaction was analyzed |
| GO:0051592 response to calcium ion | IDA PMID:12601007 Calcium- and cell cycle-dependent association of annexin 11 ... | ACCEPT | Summary: The annotated activity or location is supported as part of the core biological picture. Reason: Calcium-dependent relocalization is directly measured, supporting response to calcium ion as an existing broad process. It is not an inference that ANXA11 catalyzes calcium transport. Supporting Evidence: PMID:12601007 elevation of intracellular Ca2+ leads to translocation of annexin 11 from the nucleus to the nuclear envelope |
| GO:0051592 response to calcium ion | IDA PMID:9188810 Translocation of annexin XI to neutrophil subcellular organe... | ACCEPT | Summary: The annotated activity or location is supported as part of the core biological picture. Reason: Calcium-dependent relocalization is directly measured, supporting response to calcium ion as an existing broad process. It is not an inference that ANXA11 catalyzes calcium transport. Supporting Evidence: PMID:9188810 annexin XI translocated to specific granules and secretory vesicles/plasma membrane at 100 micromol/l calcium. PMID:9188810 When raising the concentration of calcium to 1 mmol/l, annexin XI translocated to the azurophil granules as well. |
| GO:0070062 extracellular exosome | HDA PMID:19056867 Large-scale proteomics and phosphoproteomics of urinary exos... | UNDECIDED | Summary: The exact source-specific assertion remains unresolved. Reason: The accessible source context does not expose the ANXA11-specific proteomic table or peptide evidence for this specialized compartment. Broad annexin membrane binding is not independent confirmation of this location. Retain uncertainty pending target-specific evidence, without inferring contamination, wrong-gene attribution or contradiction. |
| GO:0070062 extracellular exosome | HDA PMID:20458337 MHC class II-associated proteins in B-cell exosomes and pote... | UNDECIDED | Summary: The exact source-specific assertion remains unresolved. Reason: The accessible source context does not expose the ANXA11-specific proteomic table or peptide evidence for this specialized compartment. Broad annexin membrane binding is not independent confirmation of this location. Retain uncertainty pending target-specific evidence, without inferring contamination, wrong-gene attribution or contradiction. |
| GO:0070062 extracellular exosome | HDA PMID:23533145 In-depth proteomic analyses of exosomes isolated from expres... | UNDECIDED | Summary: The exact source-specific assertion remains unresolved. Reason: The accessible source context does not expose the ANXA11-specific proteomic table or peptide evidence for this specialized compartment. Broad annexin membrane binding is not independent confirmation of this location. Retain uncertainty pending target-specific evidence, without inferring contamination, wrong-gene attribution or contradiction. |
| GO:0043495 protein-membrane adaptor activity | IDA PMID:40118863 ANXA11 biomolecular condensates facilitate protein-lipid pha... | NEW | Summary: Human ANXA11 directly couples protein-containing RNA granules to lipid membranes. Reason: The original Methods identify full-length ANXA11 cloned from HeLa cDNA and purified after tag cleavage. Full-length ANXA11-coated giant unilamellar vesicles recruit isolated G3BP1-marked RNP granules; naked vesicles, annexin-repeat-domain-coated vesicles and purified G3BP1 alone do not reproduce this coupling. ANXA11 therefore performs the membrane-adaptor step, rather than merely being necessary for transport. This single specific molecular function covers the direct protein-complex/membrane connection; no generic adaptor ancestor or new biological process is added. Independent PMID:31539493 reconstitutes granule-liposome tethering, and PMID:42574611 demonstrates sorcin recruitment to liposomes, with their individual construct and assay limits retained. Supporting Evidence: PMID:40118863 Co-incubation of the FAPS-purified RNP granules with ANXA11-coated GUVs results in accumulation of the RNP granules onto the GUV surface PMID:40118863 RNP granules do not bind to naked GUVs or to ARD-coated GUVs PMID:40118863 Constructs encoding ANXA11 FL (aa 1-505), ANXA11 LCD (aa 1-185), ANXA11 ARD (aa 186-505), ALG2, CALC and G3BP1 were cloned by PCR amplification from HeLa cell cDNA PMID:31539493 addition of both ANXA11 and calcium promoted contact between G3BP1-positive RNA granules and liposomes PMID:42574611 sorcin binds directly and specifically to ANXA11 and that ANXA11 is sufficient to recruit sorcin to membranes in the presence of Ca2+ |
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Download this section (compressed HTML)Q: Which target-specific supplementary records support the extracellular matrix, melanosome, exosome and MHC-II-associated assertions?
Q: Does ANXA11 perform a defined phagocytic step beyond calcium-dependent recruitment to the phagosomal region?
Q: How do the two human alternative products differ in membrane and RNP-granule interactions?
Q: What molecular interaction gives ANXA11 its stable midbody role, beyond the demonstrated direct-or-indirect CHO1 association?
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