AP1G1 encodes gamma-1 adaptin, a large subunit of the heterotetrameric AP-1 clathrin adaptor complex. It helps couple cargo sorting at the trans-Golgi network and endosomes to coat assembly: the gamma/sigma hemicomplex recognizes acidic dileucine signals, while gamma hinge and appendage regions engage clathrin and accessory proteins. AP-1 cycles between cytosol and peripheral membrane coats and supports intracellular vesicular transport and recycling. Gamma-1 participates in specialized routes to melanosomes and lytic granules, and in the epithelial AP-1B complex. Pathogenic AP1G1 variants can disturb endosomal recycling and cause neurodevelopmental disease.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0000139 Golgi membrane | TAS Reactome:R-HSA-421831 | ACCEPT | Summary: Retain Golgi membrane as part of AP-1 adaptor function. Reason: AP-1 coat recruitment at Golgi/TGN membranes is independently established; retain the historical Reactome location without treating its reaction summary as a target localization experiment. Supporting Evidence: PMID:12773381 Endogenous or ectopically expressed gamma(1)- adaptin localized to both the trans-Golgi network and endosomes. PMID:15377783 directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells |
| GO:0000139 Golgi membrane | TAS Reactome:R-HSA-421833 | ACCEPT | Summary: Retain Golgi membrane as part of AP-1 adaptor function. Reason: AP-1 coat recruitment at Golgi/TGN membranes is independently established; retain the historical Reactome location without treating its reaction summary as a target localization experiment. Supporting Evidence: PMID:12773381 Endogenous or ectopically expressed gamma(1)- adaptin localized to both the trans-Golgi network and endosomes. PMID:15377783 directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells |
| GO:0000139 Golgi membrane | TAS Reactome:R-HSA-421835 | ACCEPT | Summary: Retain Golgi membrane as part of AP-1 adaptor function. Reason: AP-1 coat recruitment at Golgi/TGN membranes is independently established; retain the historical Reactome location without treating its reaction summary as a target localization experiment. Supporting Evidence: PMID:12773381 Endogenous or ectopically expressed gamma(1)- adaptin localized to both the trans-Golgi network and endosomes. PMID:15377783 directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells |
| GO:0000139 Golgi membrane | TAS Reactome:R-HSA-432706 | ACCEPT | Summary: Retain Golgi membrane as part of AP-1 adaptor function. Reason: AP-1 coat recruitment at Golgi/TGN membranes is independently established; retain the historical Reactome location without treating its reaction summary as a target localization experiment. Supporting Evidence: PMID:12773381 Endogenous or ectopically expressed gamma(1)- adaptin localized to both the trans-Golgi network and endosomes. PMID:15377783 directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells |
| GO:0000139 Golgi membrane | TAS Reactome:R-HSA-432707 | ACCEPT | Summary: Retain Golgi membrane as part of AP-1 adaptor function. Reason: AP-1 coat recruitment at Golgi/TGN membranes is independently established; retain the historical Reactome location without treating its reaction summary as a target localization experiment. Supporting Evidence: PMID:12773381 Endogenous or ectopically expressed gamma(1)- adaptin localized to both the trans-Golgi network and endosomes. PMID:15377783 directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells |
| GO:0000139 Golgi membrane | TAS Reactome:R-HSA-432712 | ACCEPT | Summary: Retain Golgi membrane as part of AP-1 adaptor function. Reason: AP-1 coat recruitment at Golgi/TGN membranes is independently established; retain the historical Reactome location without treating its reaction summary as a target localization experiment. Supporting Evidence: PMID:12773381 Endogenous or ectopically expressed gamma(1)- adaptin localized to both the trans-Golgi network and endosomes. PMID:15377783 directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells |
| GO:0005515 protein binding | IPI PMID:12505986 Divalent interaction of the GGAs with the Rabaptin-5-Rabex-5... | KEEP AS NON CORE | Summary: Retain protein binding as a supported secondary aspect. Reason: Rabaptin-5 binds the gamma appendage through its FGPLV motif (12505986 abstract; separate PMID:12042876 human gamma-ear structural corroboration). Retain this partner association as non-core; no GTPase binding/activity follows from binding a Rab effector. Supporting Evidence: PMID:12505986 an FGPLV sequence (residues 439-443) in a predicted random coil of Rabaptin-5 (a sequence also recognized by the gamma1- and gamma2-adaptin ears) PMID:12042876 Structure-based mutational analyses reveal a binding site for the accessory proteins that is composed of conserved basic residues |
| GO:0005515 protein binding | IPI PMID:15598649 AP-1 and AP-3 facilitate lysosomal targeting of Batten disea... | MODIFY | Summary: Specify contribution to cargo recognition by the AP-1 clathrin adaptor. Reason: The original PMID:15598649 construct and pull-down Methods and Figure 6 Results show human CLN3 loop material capturing native gamma-containing AP-1 from HeLa cytosol; L253A/I254A abolishes capture. Mouse-liver binding and mu1A-deficient mouse-cell sorting are separate experiments. This refines the cargo-recognition contribution of AP1G1 within assembled AP-1. It does not imply a purified gamma/sigma assay in this paper or autonomous cargo-to-clathrin bridging by isolated gamma. The normal cache remains abstract-only; the original-body locator and read scope are recorded in notes. Proposed replacements: clathrin-cargo adaptor activity Supporting Evidence: PMID:15598649 The dileucine motif of CLN3 bound both AP-1 and AP-3 in vitro |
| GO:0005515 protein binding | IPI PMID:16162817 Functions of adaptor protein (AP)-3 and AP-1 in tyrosinase s... | MODIFY | Summary: Specify contribution to cargo recognition by the AP-1 clathrin adaptor. Reason: The original PMID:16162817 Y3H and GST motif-mutant experiments demonstrate gamma1/sigma1A recognition of the tyrosinase sorting signal; immunoelectron microscopy places AP-1 and tyrosinase in clathrin-coated endosomal buds. This supports gamma contribution to cargo selection by the assembled clathrin adaptor. Mouse tyrosinase-tail reagents, inherited gamma constructs, human MNT-1 cells and mouse melanocytes remain distinct. No isolated gamma recognition interface or autonomous bridge is asserted. Proposed replacements: clathrin-cargo adaptor activity Supporting Evidence: PMID:16162817 Both adaptors recognize the tyrosinase dileucine-based melanosome sorting signal |
| GO:0005515 protein binding | IPI PMID:19116314 Localization to mature melanosomes by virtue of cytoplasmic ... | MODIFY | Summary: Specify contribution to cargo recognition by the AP-1 clathrin adaptor. Reason: The original PMID:19116314 Methods and Results, read in the separately preserved fuller source, show human OCA2-tail capture of native AP-1 from HeLa/MNT-1 extracts with dileucine mutation controls. This refines the cargo-recognition contribution within assembled AP-1, not purified gamma-only binding. Gamma coat contacts are separately established by the existing core evidence; the OCA2 assay is not represented as a complete isolated gamma bridge. The normal cache remains abstract-only and the actual original-body locator is recorded in notes. Proposed replacements: clathrin-cargo adaptor activity Supporting Evidence: file:human/AP1G1/AP1G1-notes.md GST-OCA2 NT bound the adaptor proteins AP-1, AP-2, and AP-3 |
| GO:0005515 protein binding | IPI PMID:26496610 A human interactome in three quantitative dimensions organiz... | UNDECIDED | Summary: The source-specific gamma1βsigma1B interaction remains unadjudicated. Reason: The exact original screen pair, variant and construct record for AP1G1/AP1S2 was not inspected. The previous PMID:9733768 quotation described gamma2/sigma1B alongside a gamma1 comparison and did not unambiguously establish this gamma1/sigma1B pair. Independently inspected PMID:34102099 main text describes association with sigma1 generically; it does not settle the original screen reagent or assay. Preserve the source assertion as unresolved without denying that AP1G1 can associate with AP1S2. |
| GO:0005515 protein binding | IPI PMID:27293189 Restricted Location of PSEN2/Ξ³-Secretase Determines Substrat... | MODIFY | Summary: Specify contribution to cargo recognition by the AP-1 clathrin adaptor. Reason: The original PMID:27293189 Results establish PSEN2 acidic sorting-motif recognition by gamma1/sigma1 in Y3H, native AP-1 capture from rat brain and motif-dependent targeting in clathrin-coated carrier context. This supports gamma contribution to clathrin-cargo adaptor activity within the assembled complex. Human PSEN2 constructs and rat extract are distinct; no autonomous gamma interface or PSEN2 protease activity is attributed to AP1G1. Proposed replacements: clathrin-cargo adaptor activity Supporting Evidence: PMID:27293189 a unique motif in PSEN2 that directs this Ξ³-secretase to late endosomes/lysosomes via a phosphorylation-dependent interaction with the AP-1 adaptor complex |
| GO:0005515 protein binding | IPI PMID:29892012 An interactome perturbation framework prioritizes damaging m... | UNDECIDED | Summary: The source-specific gamma1βsigma1B interaction remains unadjudicated. Reason: The exact original screen pair, variant and construct record for AP1G1/AP1S2 was not inspected. The previous PMID:9733768 quotation described gamma2/sigma1B alongside a gamma1 comparison and did not unambiguously establish this gamma1/sigma1B pair. Independently inspected PMID:34102099 main text describes association with sigma1 generically; it does not settle the original screen reagent or assay. Preserve the source assertion as unresolved without denying that AP1G1 can associate with AP1S2. |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | UNDECIDED | Summary: The source-specific gamma1βsigma1B interaction remains unadjudicated. Reason: The exact original screen pair, variant and construct record for AP1G1/AP1S2 was not inspected. The previous PMID:9733768 quotation described gamma2/sigma1B alongside a gamma1 comparison and did not unambiguously establish this gamma1/sigma1B pair. Independently inspected PMID:34102099 main text describes association with sigma1 generically; it does not settle the original screen reagent or assay. Preserve the source assertion as unresolved without denying that AP1G1 can associate with AP1S2. |
| GO:0005518 collagen binding | IEA GO_REF:0000107 | UNDECIDED | Summary: The source-specific collagen binding assertion remains unresolved. Reason: Rat donor accession resolves to Ap1g1, but the collagen experiment and exact electronic transfer were not recovered. Intracellular localization alone does not refute binding to collagen/cargo. Remain uncertain rather than claim a wrong gene or impossible compartment. Propagation Review Root cause: UNRESOLVED Sources checked: UniProtKB:A0A8I5Y697 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. ensembl:ENSRNOP00000077402 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. |
| GO:0005737 cytoplasm | EXP PMID:15758025 The aftiphilin/p200/gamma-synergin complex. | ACCEPT | Summary: Retain cytoplasm as part of AP-1 adaptor function. Reason: Retain the source EXP location while distinguishing its own scope from independent corroboration. PMID:15758025 concerns the aftiphilin/p200/gamma-synergin accessory complex; its preserved normal abstract does not expose the exact gamma cytoplasmic experiment. The separately checked human HPA assay table directly supports the cytosolic pool, consistent with AP-1 recruitment. Defer to the original location curation without presenting an accessory-protein phenotype or a different PMID as this experiment. Supporting Evidence: file:human/AP1G1/AP1G1-notes.md Mainly localized to the Golgi apparatus and vesicles. In addition localized to the cytosol. |
| GO:0005737 cytoplasm | IEA GO_REF:0000044 | ACCEPT | Summary: Retain cytoplasm as part of AP-1 adaptor function. Reason: The UniProt subcellular-location mapping agrees with the independently inspected human HPA cytosolic pool and AP-1 membrane recruitment. The precise electronic derivation was not reconstructed, but the target location is independently corroborated; no inference is made from a paralog-focused title. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB-SubCell:SL-0086 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. Supporting Evidence: file:human/AP1G1/AP1G1-notes.md Mainly localized to the Golgi apparatus and vesicles. In addition localized to the cytosol. |
| GO:0005737 cytoplasm | TAS PMID:9733768 Identification and characterization of novel clathrin adapto... | ACCEPT | Summary: Retain cytoplasm as part of AP-1 adaptor function. Reason: PMID:9733768 explicitly includes gamma1 comparator context, although its preserved abstract does not resolve the exact cytoplasmic localization experiment. The independently inspected human HPA assay table supports the cytosolic pool. Retain the existing TAS location with that source-specific limit rather than treating the gamma2-focused title as evidence against gamma1. Supporting Evidence: file:human/AP1G1/AP1G1-notes.md Mainly localized to the Golgi apparatus and vesicles. In addition localized to the cytosol. |
| GO:0005765 lysosomal membrane | NAS PMID:23247405 Cell type-specific Rab32 and Rab38 cooperate with the ubiqui... | UNDECIDED | Summary: The source-specific lysosomal membrane assertion remains unresolved. Reason: The reviewed source contexts concern lysosome-destined transport carriers and coat removal before fusion, not a clearly resolved mature lysosomal-membrane pool. 23247405 describes early/recycling-endosomal sorting; Reactome432688 uncoating and432707 scission summaries do not resolve target gamma on lysosomal membrane. Request source-specific clarification; do not infer localization merely from cargo destination or claim global absence. |
| GO:0005765 lysosomal membrane | TAS Reactome:R-HSA-432688 | UNDECIDED | Summary: The source-specific lysosomal membrane assertion remains unresolved. Reason: The reviewed source contexts concern lysosome-destined transport carriers and coat removal before fusion, not a clearly resolved mature lysosomal-membrane pool. 23247405 describes early/recycling-endosomal sorting; Reactome432688 uncoating and432707 scission summaries do not resolve target gamma on lysosomal membrane. Request source-specific clarification; do not infer localization merely from cargo destination or claim global absence. |
| GO:0005765 lysosomal membrane | TAS Reactome:R-HSA-432707 | UNDECIDED | Summary: The source-specific lysosomal membrane assertion remains unresolved. Reason: The reviewed source contexts concern lysosome-destined transport carriers and coat removal before fusion, not a clearly resolved mature lysosomal-membrane pool. 23247405 describes early/recycling-endosomal sorting; Reactome432688 uncoating and432707 scission summaries do not resolve target gamma on lysosomal membrane. Request source-specific clarification; do not infer localization merely from cargo destination or claim global absence. |
| GO:0005768 endosome | IEA GO_REF:0000117 | ACCEPT | Summary: Retain endosome as part of AP-1 adaptor function. Reason: Target AP-1 function/localization at early and recycling endosomal tubules is directly corroborated by PMID:19841138 and PMID:22511774. Retain biological location while exact ARBA derivation, where present, remains unresolved. Propagation Review Root cause: NO FAILURE CORE Sources checked: ARBA:ARBA00028568 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. Supporting Evidence: PMID:19841138 the clathrin adaptor AP-1 and the kinesin motor KIF13A together create peripheral recycling endosomal subdomains in melanocytes PMID:12773381 Endogenous or ectopically expressed gamma(1)- adaptin localized to both the trans-Golgi network and endosomes. |
| GO:0005769 early endosome | NAS PMID:23247405 Cell type-specific Rab32 and Rab38 cooperate with the ubiqui... | ACCEPT | Summary: Retain early endosome as part of AP-1 adaptor function. Reason: Target AP-1 function/localization at early and recycling endosomal tubules is directly corroborated by PMID:19841138 and PMID:22511774. Retain biological location while exact ARBA derivation, where present, remains unresolved. Supporting Evidence: PMID:19841138 the clathrin adaptor AP-1 and the kinesin motor KIF13A together create peripheral recycling endosomal subdomains in melanocytes PMID:12773381 Endogenous or ectopically expressed gamma(1)- adaptin localized to both the trans-Golgi network and endosomes. |
| GO:0005794 Golgi apparatus | EXP PMID:12773381 Rabaptin-5alpha/rabaptin-4 serves as a linker between rab4 a... | ACCEPT | Summary: Retain Golgi apparatus as part of AP-1 adaptor function. Reason: Golgi/TGN localization is supported by target experiments and actual HPA table. Different source provenance remains distinct: InterPro/rule and mouse transfer derivations are not reconstructed, and the gamma2-focused title does not exclude gamma1 comparators. Supporting Evidence: PMID:12773381 Endogenous or ectopically expressed gamma(1)- adaptin localized to both the trans-Golgi network and endosomes. PMID:15377783 directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells |
| GO:0005794 Golgi apparatus | IDA GO_REF:0000052 | ACCEPT | Summary: Retain Golgi apparatus as part of AP-1 adaptor function. Reason: Golgi/TGN localization is supported by target experiments and actual HPA table. Different source provenance remains distinct: InterPro/rule and mouse transfer derivations are not reconstructed, and the gamma2-focused title does not exclude gamma1 comparators. Supporting Evidence: PMID:12773381 Endogenous or ectopically expressed gamma(1)- adaptin localized to both the trans-Golgi network and endosomes. PMID:15377783 directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells |
| GO:0005794 Golgi apparatus | IDA PMID:9733768 Identification and characterization of novel clathrin adapto... | ACCEPT | Summary: Retain Golgi apparatus as part of AP-1 adaptor function. Reason: Golgi/TGN localization is supported by target experiments and actual HPA table. Different source provenance remains distinct: InterPro/rule and mouse transfer derivations are not reconstructed, and the gamma2-focused title does not exclude gamma1 comparators. Supporting Evidence: PMID:12773381 Endogenous or ectopically expressed gamma(1)- adaptin localized to both the trans-Golgi network and endosomes. PMID:15377783 directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells |
| GO:0005794 Golgi apparatus | IEA GO_REF:0000120 | ACCEPT | Summary: Retain Golgi apparatus as part of AP-1 adaptor function. Reason: Golgi/TGN localization is supported by target experiments and actual HPA table. Different source provenance remains distinct: InterPro/rule and mouse transfer derivations are not reconstructed, and the gamma2-focused title does not exclude gamma1 comparators. Propagation Review Root cause: NO FAILURE CORE Sources checked: InterPro:IPR017107 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. UniProtKB-SubCell:SL-0132 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. Supporting Evidence: PMID:12773381 Endogenous or ectopically expressed gamma(1)- adaptin localized to both the trans-Golgi network and endosomes. PMID:15377783 directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells |
| GO:0005802 trans-Golgi network | IEA GO_REF:0000120 | ACCEPT | Summary: Retain trans-Golgi network as part of AP-1 adaptor function. Reason: Golgi/TGN localization is supported by target experiments and actual HPA table. Different source provenance remains distinct: InterPro/rule and mouse transfer derivations are not reconstructed, and the gamma2-focused title does not exclude gamma1 comparators. Propagation Review Root cause: NO FAILURE CORE Sources checked: ARBA:ARBA00029112 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. UniProtKB:P22892 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. ensembl:ENSMUSP00000034171 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. Supporting Evidence: PMID:12773381 Endogenous or ectopically expressed gamma(1)- adaptin localized to both the trans-Golgi network and endosomes. PMID:15377783 directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells |
| GO:0005829 cytosol | IDA GO_REF:0000052 | ACCEPT | Summary: Retain cytosol as part of AP-1 adaptor function. Reason: The soluble cytosolic pool is directly corroborated by HPA and AP-1 recruitment/complex biochemistry. Reactome pathway association is retained as contextual evidence, not a separate experiment for every event. Supporting Evidence: file:human/AP1G1/AP1G1-notes.md Mainly localized to the Golgi apparatus and vesicles. In addition localized to the cytosol. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-182263 | ACCEPT | Summary: Retain cytosol as part of AP-1 adaptor function. Reason: The soluble cytosolic pool is directly corroborated by HPA and AP-1 recruitment/complex biochemistry. Reactome pathway association is retained as contextual evidence, not a separate experiment for every event. Supporting Evidence: file:human/AP1G1/AP1G1-notes.md Mainly localized to the Golgi apparatus and vesicles. In addition localized to the cytosol. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-182279 | ACCEPT | Summary: Retain cytosol as part of AP-1 adaptor function. Reason: The soluble cytosolic pool is directly corroborated by HPA and AP-1 recruitment/complex biochemistry. Reactome pathway association is retained as contextual evidence, not a separate experiment for every event. Supporting Evidence: file:human/AP1G1/AP1G1-notes.md Mainly localized to the Golgi apparatus and vesicles. In addition localized to the cytosol. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-182286 | ACCEPT | Summary: Retain cytosol as part of AP-1 adaptor function. Reason: The soluble cytosolic pool is directly corroborated by HPA and AP-1 recruitment/complex biochemistry. Reactome pathway association is retained as contextual evidence, not a separate experiment for every event. Supporting Evidence: file:human/AP1G1/AP1G1-notes.md Mainly localized to the Golgi apparatus and vesicles. In addition localized to the cytosol. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-2130619 | ACCEPT | Summary: Retain cytosol as part of AP-1 adaptor function. Reason: The soluble cytosolic pool is directly corroborated by HPA and AP-1 recruitment/complex biochemistry. Reactome pathway association is retained as contextual evidence, not a separate experiment for every event. Supporting Evidence: file:human/AP1G1/AP1G1-notes.md Mainly localized to the Golgi apparatus and vesicles. In addition localized to the cytosol. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-2213236 | ACCEPT | Summary: Retain cytosol as part of AP-1 adaptor function. Reason: The soluble cytosolic pool is directly corroborated by HPA and AP-1 recruitment/complex biochemistry. Reactome pathway association is retained as contextual evidence, not a separate experiment for every event. Supporting Evidence: file:human/AP1G1/AP1G1-notes.md Mainly localized to the Golgi apparatus and vesicles. In addition localized to the cytosol. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-421833 | ACCEPT | Summary: Retain cytosol as part of AP-1 adaptor function. Reason: The soluble cytosolic pool is directly corroborated by HPA and AP-1 recruitment/complex biochemistry. Reactome pathway association is retained as contextual evidence, not a separate experiment for every event. Supporting Evidence: file:human/AP1G1/AP1G1-notes.md Mainly localized to the Golgi apparatus and vesicles. In addition localized to the cytosol. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-421836 | ACCEPT | Summary: Retain cytosol as part of AP-1 adaptor function. Reason: The soluble cytosolic pool is directly corroborated by HPA and AP-1 recruitment/complex biochemistry. Reactome pathway association is retained as contextual evidence, not a separate experiment for every event. Supporting Evidence: file:human/AP1G1/AP1G1-notes.md Mainly localized to the Golgi apparatus and vesicles. In addition localized to the cytosol. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-432688 | ACCEPT | Summary: Retain cytosol as part of AP-1 adaptor function. Reason: The soluble cytosolic pool is directly corroborated by HPA and AP-1 recruitment/complex biochemistry. Reactome pathway association is retained as contextual evidence, not a separate experiment for every event. Supporting Evidence: file:human/AP1G1/AP1G1-notes.md Mainly localized to the Golgi apparatus and vesicles. In addition localized to the cytosol. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-432712 | ACCEPT | Summary: Retain cytosol as part of AP-1 adaptor function. Reason: The soluble cytosolic pool is directly corroborated by HPA and AP-1 recruitment/complex biochemistry. Reactome pathway association is retained as contextual evidence, not a separate experiment for every event. Supporting Evidence: file:human/AP1G1/AP1G1-notes.md Mainly localized to the Golgi apparatus and vesicles. In addition localized to the cytosol. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8951498 | ACCEPT | Summary: Retain cytosol as part of AP-1 adaptor function. Reason: The soluble cytosolic pool is directly corroborated by HPA and AP-1 recruitment/complex biochemistry. Reactome pathway association is retained as contextual evidence, not a separate experiment for every event. Supporting Evidence: file:human/AP1G1/AP1G1-notes.md Mainly localized to the Golgi apparatus and vesicles. In addition localized to the cytosol. |
| GO:0005905 clathrin-coated pit | IEA GO_REF:0000044 | ACCEPT | Summary: Retain clathrin-coated pit as part of AP-1 adaptor function. Reason: Clathrin-coated pits are not restricted by the GO label to plasma-membrane AP-2 pits. Gamma1 acts in intracellular AP-1 clathrin-coated budding structures. Retain the coat-associated location without reassigning AP-1 to canonical AP-2 uptake. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB-SubCell:SL-0069 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. Supporting Evidence: PMID:11451993 the hinge and appendage domains of gamma function in a cooperative manner to recruit and polymerize clathrin PMID:15377783 directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells |
| GO:0006886 intracellular protein transport | IEA GO_REF:0000002 | ACCEPT | Summary: Retain intracellular protein transport as part of AP-1 adaptor function. Reason: Gamma1 contributes to AP-1 sorting/coating of cargo carriers; direct cargo recognition and coat assembly establish participation in the existing broad transport process. This is not a NEW process inferred solely from perturbation. Propagation Review Root cause: NO FAILURE CORE Sources checked: InterPro:IPR002553 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. InterPro:IPR008152 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. Supporting Evidence: PMID:17360967 the gamma/sigma1 or alpha/sigma2 hemicomplexes bound the dileucine-based motifs of several proteins quite strongly PMID:23415225 AP-1 is a clathrin adaptor complex that sorts cargo between the trans-Golgi network and endosomes. |
| GO:0016020 membrane | HDA PMID:19946888 Defining the membrane proteome of NK cells. | ACCEPT | Summary: Retain membrane as part of AP-1 adaptor function. Reason: Broad membrane association is independently demonstrated by membrane-bound AP-1 coat recruitment; the NK-cell proteomics target peptide entry remains uninspected. No integral transmembrane topology is implied. Supporting Evidence: PMID:11451993 the hinge and appendage domains of gamma function in a cooperative manner to recruit and polymerize clathrin PMID:15377783 directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells |
| GO:0016182 synaptic vesicle budding from endosome | IEA GO_REF:0000107 | UNDECIDED | Summary: The source-specific synaptic vesicle budding from endosome assertion remains unresolved. Reason: Exact donor experiment and target-specific presynaptic/synaptic-vesicle function were not recovered. AP-1 specialization alone does not prove these particular neuronal routes, but it also does not refute a conserved neuronal role. Preserve the electronic assertion as uncertain; no donor-count/qualifier inference. Propagation Review Root cause: UNRESOLVED Sources checked: UniProtKB:A0A8I5Y697 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. ensembl:ENSRNOP00000077402 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. |
| GO:0016192 vesicle-mediated transport | IEA GO_REF:0000120 | ACCEPT | Summary: Retain vesicle-mediated transport as part of AP-1 adaptor function. Reason: Gamma1 contributes to AP-1 sorting/coating of cargo carriers; direct cargo recognition and coat assembly establish participation in the existing broad transport process. This is not a NEW process inferred solely from perturbation. Propagation Review Root cause: NO FAILURE CORE Sources checked: ARBA:ARBA00028249 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. InterPro:IPR002553 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. InterPro:IPR008152 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. InterPro:IPR017107 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. Supporting Evidence: PMID:17360967 the gamma/sigma1 or alpha/sigma2 hemicomplexes bound the dileucine-based motifs of several proteins quite strongly PMID:23415225 AP-1 is a clathrin adaptor complex that sorts cargo between the trans-Golgi network and endosomes. |
| GO:0016192 vesicle-mediated transport | NAS PMID:23247405 Cell type-specific Rab32 and Rab38 cooperate with the ubiqui... | ACCEPT | Summary: Retain vesicle-mediated transport as part of AP-1 adaptor function. Reason: Gamma1 contributes to AP-1 sorting/coating of cargo carriers; direct cargo recognition and coat assembly establish participation in the existing broad transport process. This is not a NEW process inferred solely from perturbation. Supporting Evidence: PMID:17360967 the gamma/sigma1 or alpha/sigma2 hemicomplexes bound the dileucine-based motifs of several proteins quite strongly PMID:23415225 AP-1 is a clathrin adaptor complex that sorts cargo between the trans-Golgi network and endosomes. |
| GO:0016192 vesicle-mediated transport | NAS PMID:27057418 Role of the epithelial cell-specific clathrin adaptor comple... | ACCEPT | Summary: Retain vesicle-mediated transport as part of AP-1 adaptor function. Reason: Gamma1 contributes to AP-1 sorting/coating of cargo carriers; direct cargo recognition and coat assembly establish participation in the existing broad transport process. This is not a NEW process inferred solely from perturbation. Supporting Evidence: PMID:17360967 the gamma/sigma1 or alpha/sigma2 hemicomplexes bound the dileucine-based motifs of several proteins quite strongly PMID:23415225 AP-1 is a clathrin adaptor complex that sorts cargo between the trans-Golgi network and endosomes. |
| GO:0019894 kinesin binding | IPI PMID:19841138 AP-1 and KIF13A coordinate endosomal sorting and positioning... | KEEP AS NON CORE | Summary: Retain kinesin binding as a supported secondary aspect. Reason: 19841138 actual human MNT1 reciprocal gamma/KIF13A association supports kinesin binding at the existing complex-association scope. Do not infer a purified binary interface or create a second motor-adaptor MF from co-IP alone; retain as specialized non-core association. Supporting Evidence: PMID:19841138 Ξ³-adaptin is coimmunoprecipitated with anti-KIF13A |
| GO:0030117 membrane coat | IEA GO_REF:0000002 | ACCEPT | Summary: Retain membrane coat as part of AP-1 adaptor function. Reason: Gamma1 is an integral AP-1 subunit participating in the clathrin coat. Exact IBD topology and electronic derivations remain unresolved, but independent AP-1 structural/biochemical evidence supports the target assertion. No short-donor-list objection. Propagation Review Root cause: NO FAILURE CORE Sources checked: InterPro:IPR002553 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. Supporting Evidence: PMID:15377783 The AP-1 core comprises N-terminal fragments of the two large chains, beta1 and gamma, and the intact medium and small chains, micro1 and sigma1. |
| GO:0030121 AP-1 adaptor complex | IBA GO_REF:0000033 | ACCEPT | Summary: Retain AP-1 adaptor complex as part of AP-1 adaptor function. Reason: Gamma1 is an integral AP-1 subunit participating in the clathrin coat. Exact IBD topology and electronic derivations remain unresolved, but independent AP-1 structural/biochemical evidence supports the target assertion. No short-donor-list objection. Propagation Review Root cause: NO FAILURE CORE Sources checked: FB:FBgn0030089 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. PANTHER:PTN000512793 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. PomBase:SPCP1E11.06 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. SGD:S000006233 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. dictyBase:DDB_G0281957 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. Supporting Evidence: PMID:15377783 The AP-1 core comprises N-terminal fragments of the two large chains, beta1 and gamma, and the intact medium and small chains, micro1 and sigma1. |
| GO:0030121 AP-1 adaptor complex | IEA GO_REF:0000002 | ACCEPT | Summary: Retain AP-1 adaptor complex as part of AP-1 adaptor function. Reason: Gamma1 is an integral AP-1 subunit participating in the clathrin coat. Exact IBD topology and electronic derivations remain unresolved, but independent AP-1 structural/biochemical evidence supports the target assertion. No short-donor-list objection. Propagation Review Root cause: NO FAILURE CORE Sources checked: InterPro:IPR017107 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. Supporting Evidence: PMID:15377783 The AP-1 core comprises N-terminal fragments of the two large chains, beta1 and gamma, and the intact medium and small chains, micro1 and sigma1. |
| GO:0030121 AP-1 adaptor complex | NAS PMID:15377783 Crystal structure of the clathrin adaptor protein 1 core. | ACCEPT | Summary: Retain AP-1 adaptor complex as part of AP-1 adaptor function. Reason: Gamma1 is an integral AP-1 subunit participating in the clathrin coat. Exact IBD topology and electronic derivations remain unresolved, but independent AP-1 structural/biochemical evidence supports the target assertion. No short-donor-list objection. Supporting Evidence: PMID:15377783 The AP-1 core comprises N-terminal fragments of the two large chains, beta1 and gamma, and the intact medium and small chains, micro1 and sigma1. |
| GO:0030121 AP-1 adaptor complex | NAS PMID:27057418 Role of the epithelial cell-specific clathrin adaptor comple... | ACCEPT | Summary: Retain AP-1 adaptor complex as part of AP-1 adaptor function. Reason: Gamma1 is an integral AP-1 subunit participating in the clathrin coat. Exact IBD topology and electronic derivations remain unresolved, but independent AP-1 structural/biochemical evidence supports the target assertion. No short-donor-list objection. Supporting Evidence: PMID:15377783 The AP-1 core comprises N-terminal fragments of the two large chains, beta1 and gamma, and the intact medium and small chains, micro1 and sigma1. |
| GO:0030136 clathrin-coated vesicle | IDA PMID:12536145 Identification of a novel domain in two mammalian inositol-p... | ACCEPT | Summary: Retain clathrin-coated vesicle as part of AP-1 adaptor function. Reason: The exact AP1G1 experiment underlying the PMID:12536145 IDA is not exposed by the preserved abstract. Retain the curated location with separate gamma clathrin-recruitment evidence and AP-1 coat localization; abstract silence does not establish a misattribution. Mouse gamma fragments, rat cytosol and bovine purified clathrin in the independent study are distinguished rather than described as a human purified assay. Supporting Evidence: PMID:11451993 the hinge and appendage domains of gamma function in a cooperative manner to recruit and polymerize clathrin PMID:15377783 directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells |
| GO:0030136 clathrin-coated vesicle | IEA GO_REF:0000044 | ACCEPT | Summary: Retain clathrin-coated vesicle as part of AP-1 adaptor function. Reason: The UniProt clathrin-coated-vesicle mapping is consistent with independently inspected AP-1 coat biochemistry and localization. The precise electronic mapping was not reconstructed. Its corroboration is separate from the unresolved source-local experiment behind the adjacent PMID:12536145 IDA. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB-SubCell:SL-0070 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. Supporting Evidence: PMID:11451993 the hinge and appendage domains of gamma function in a cooperative manner to recruit and polymerize clathrin PMID:15377783 directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells |
| GO:0030659 cytoplasmic vesicle membrane | IEA GO_REF:0000117 | ACCEPT | Summary: Retain cytoplasmic vesicle membrane as part of AP-1 adaptor function. Reason: Independent AP-1 clathrin-coated-carrier biochemistry and target trafficking evidence corroborate this location. Abstract-only12536145 does not expose the AP1G1 experiment, so defer to its curated IDA with independent target support rather than reject from title silence. Do not equate species of every ortholog reagent. Propagation Review Root cause: NO FAILURE CORE Sources checked: ARBA:ARBA00026540 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. Supporting Evidence: PMID:11451993 the hinge and appendage domains of gamma function in a cooperative manner to recruit and polymerize clathrin PMID:15377783 directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells |
| GO:0030659 cytoplasmic vesicle membrane | TAS Reactome:R-HSA-421835 | ACCEPT | Summary: Retain cytoplasmic vesicle membrane as part of AP-1 adaptor function. Reason: Independent AP-1 clathrin-coated-carrier biochemistry and target trafficking evidence corroborate this location. Abstract-only12536145 does not expose the AP1G1 experiment, so defer to its curated IDA with independent target support rather than reject from title silence. Do not equate species of every ortholog reagent. Supporting Evidence: PMID:11451993 the hinge and appendage domains of gamma function in a cooperative manner to recruit and polymerize clathrin PMID:15377783 directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells |
| GO:0030659 cytoplasmic vesicle membrane | TAS Reactome:R-HSA-421836 | ACCEPT | Summary: Retain cytoplasmic vesicle membrane as part of AP-1 adaptor function. Reason: Independent AP-1 clathrin-coated-carrier biochemistry and target trafficking evidence corroborate this location. Abstract-only12536145 does not expose the AP1G1 experiment, so defer to its curated IDA with independent target support rather than reject from title silence. Do not equate species of every ortholog reagent. Supporting Evidence: PMID:11451993 the hinge and appendage domains of gamma function in a cooperative manner to recruit and polymerize clathrin PMID:15377783 directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells |
| GO:0030665 clathrin-coated vesicle membrane | IEA GO_REF:0000044 | ACCEPT | Summary: Retain clathrin-coated vesicle membrane as part of AP-1 adaptor function. Reason: Independent AP-1 clathrin-coated-carrier biochemistry and target trafficking evidence corroborate this location. Abstract-only12536145 does not expose the AP1G1 experiment, so defer to its curated IDA with independent target support rather than reject from title silence. Do not equate species of every ortholog reagent. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB-SubCell:SL-0071 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. Supporting Evidence: PMID:11451993 the hinge and appendage domains of gamma function in a cooperative manner to recruit and polymerize clathrin PMID:15377783 directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells |
| GO:0030665 clathrin-coated vesicle membrane | ISS GO_REF:0000024 | ACCEPT | Summary: Retain clathrin-coated vesicle membrane as part of AP-1 adaptor function. Reason: Independent AP-1 clathrin-coated-carrier biochemistry and target trafficking evidence corroborate this location. Abstract-only12536145 does not expose the AP1G1 experiment, so defer to its curated IDA with independent target support rather than reject from title silence. Do not equate species of every ortholog reagent. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:Q8R525 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. Supporting Evidence: PMID:11451993 the hinge and appendage domains of gamma function in a cooperative manner to recruit and polymerize clathrin PMID:15377783 directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells |
| GO:0030742 GTP-dependent protein binding | IPI PMID:22511774 BLOC-2, AP-3, and AP-1 proteins function in concert with Rab... | KEEP AS NON CORE | Summary: Retain GTP-dependent protein binding as a supported secondary aspect. Reason: 22511774 richer original extraction shows active-state-selective Rab32/Rab38 association with AP-1 from cell extracts, alongside target gamma knockdown. Retain the existing GTP-dependent/small-GTPase association at complex scope; bridging is not excluded and no gamma GEF/GAP/catalysis is inferred. This specialized melanosomal association is non-core. Supporting Evidence: file:human/AP1G1/AP1G1-notes.md AP-1 also showed a preference for the GTPΞ³S form of Rab32 and Rab38 but less dramatically than BLOC-2 or AP-3 PMID:22511774 BLOC-2, AP-3, and AP-1 coimmunoprecipitated with Rab38 and Rab32 from MNT-1 melanocytic cell extracts. |
| GO:0030742 GTP-dependent protein binding | IPI PMID:22511774 BLOC-2, AP-3, and AP-1 proteins function in concert with Rab... | KEEP AS NON CORE | Summary: Retain GTP-dependent protein binding as a supported secondary aspect. Reason: 22511774 richer original extraction shows active-state-selective Rab32/Rab38 association with AP-1 from cell extracts, alongside target gamma knockdown. Retain the existing GTP-dependent/small-GTPase association at complex scope; bridging is not excluded and no gamma GEF/GAP/catalysis is inferred. This specialized melanosomal association is non-core. Supporting Evidence: file:human/AP1G1/AP1G1-notes.md AP-1 also showed a preference for the GTPΞ³S form of Rab32 and Rab38 but less dramatically than BLOC-2 or AP-3 PMID:22511774 BLOC-2, AP-3, and AP-1 coimmunoprecipitated with Rab38 and Rab32 from MNT-1 melanocytic cell extracts. |
| GO:0031267 small GTPase binding | IPI PMID:22511774 BLOC-2, AP-3, and AP-1 proteins function in concert with Rab... | KEEP AS NON CORE | Summary: Retain small GTPase binding as a supported secondary aspect. Reason: 22511774 richer original extraction shows active-state-selective Rab32/Rab38 association with AP-1 from cell extracts, alongside target gamma knockdown. Retain the existing GTP-dependent/small-GTPase association at complex scope; bridging is not excluded and no gamma GEF/GAP/catalysis is inferred. This specialized melanosomal association is non-core. Supporting Evidence: PMID:22511774 BLOC-2, AP-3, and AP-1 coimmunoprecipitated with Rab38 and Rab32 from MNT-1 melanocytic cell extracts. |
| GO:0031267 small GTPase binding | IPI PMID:22511774 BLOC-2, AP-3, and AP-1 proteins function in concert with Rab... | KEEP AS NON CORE | Summary: Retain small GTPase binding as a supported secondary aspect. Reason: 22511774 richer original extraction shows active-state-selective Rab32/Rab38 association with AP-1 from cell extracts, alongside target gamma knockdown. Retain the existing GTP-dependent/small-GTPase association at complex scope; bridging is not excluded and no gamma GEF/GAP/catalysis is inferred. This specialized melanosomal association is non-core. Supporting Evidence: PMID:22511774 BLOC-2, AP-3, and AP-1 coimmunoprecipitated with Rab38 and Rab32 from MNT-1 melanocytic cell extracts. |
| GO:0032438 melanosome organization | IC PMID:22511774 BLOC-2, AP-3, and AP-1 proteins function in concert with Rab... | KEEP AS NON CORE | Summary: Retain melanosome organization as a supported secondary aspect. Reason: AP-1-mediated cargo sorting contributes to melanosome biogenesis; PMID:22511774 gamma-specific perturbation and cargo-distribution results support the existing process assertions. Retain this specialized organelle route as non-core, with no NEW process and no implication that all coat components perform pigment chemistry. Supporting Evidence: PMID:22511774 BLOC-2, AP-3, and AP-1 coimmunoprecipitated with Rab38 and Rab32 from MNT-1 melanocytic cell extracts. |
| GO:0032588 trans-Golgi network membrane | NAS PMID:15377783 Crystal structure of the clathrin adaptor protein 1 core. | ACCEPT | Summary: Retain trans-Golgi network membrane as part of AP-1 adaptor function. Reason: TGN-membrane recruitment is central to AP-1 coat/adaptor function and independently corroborated. Reaction and review sources remain contextual rather than newly performed localization experiments. Supporting Evidence: PMID:12773381 Endogenous or ectopically expressed gamma(1)- adaptin localized to both the trans-Golgi network and endosomes. PMID:15377783 directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells |
| GO:0032588 trans-Golgi network membrane | NAS PMID:27057418 Role of the epithelial cell-specific clathrin adaptor comple... | ACCEPT | Summary: Retain trans-Golgi network membrane as part of AP-1 adaptor function. Reason: TGN-membrane recruitment is central to AP-1 coat/adaptor function and independently corroborated. Reaction and review sources remain contextual rather than newly performed localization experiments. Supporting Evidence: PMID:12773381 Endogenous or ectopically expressed gamma(1)- adaptin localized to both the trans-Golgi network and endosomes. PMID:15377783 directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells |
| GO:0032588 trans-Golgi network membrane | TAS Reactome:R-HSA-2130641 | ACCEPT | Summary: Retain trans-Golgi network membrane as part of AP-1 adaptor function. Reason: TGN-membrane recruitment is central to AP-1 coat/adaptor function and independently corroborated. Reaction and review sources remain contextual rather than newly performed localization experiments. Supporting Evidence: PMID:12773381 Endogenous or ectopically expressed gamma(1)- adaptin localized to both the trans-Golgi network and endosomes. PMID:15377783 directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells |
| GO:0032588 trans-Golgi network membrane | TAS Reactome:R-HSA-2213236 | ACCEPT | Summary: Retain trans-Golgi network membrane as part of AP-1 adaptor function. Reason: TGN-membrane recruitment is central to AP-1 coat/adaptor function and independently corroborated. Reaction and review sources remain contextual rather than newly performed localization experiments. Supporting Evidence: PMID:12773381 Endogenous or ectopically expressed gamma(1)- adaptin localized to both the trans-Golgi network and endosomes. PMID:15377783 directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells |
| GO:0032588 trans-Golgi network membrane | TAS Reactome:R-HSA-5333658 | ACCEPT | Summary: Retain trans-Golgi network membrane as part of AP-1 adaptor function. Reason: TGN-membrane recruitment is central to AP-1 coat/adaptor function and independently corroborated. Reaction and review sources remain contextual rather than newly performed localization experiments. Supporting Evidence: PMID:12773381 Endogenous or ectopically expressed gamma(1)- adaptin localized to both the trans-Golgi network and endosomes. PMID:15377783 directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells |
| GO:0032588 trans-Golgi network membrane | TAS Reactome:R-HSA-8951498 | ACCEPT | Summary: Retain trans-Golgi network membrane as part of AP-1 adaptor function. Reason: TGN-membrane recruitment is central to AP-1 coat/adaptor function and independently corroborated. Reaction and review sources remain contextual rather than newly performed localization experiments. Supporting Evidence: PMID:12773381 Endogenous or ectopically expressed gamma(1)- adaptin localized to both the trans-Golgi network and endosomes. PMID:15377783 directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells |
| GO:0035615 clathrin-cargo adaptor activity | IBA GO_REF:0000033 | ACCEPT | Summary: Retain clathrin-cargo adaptor activity as part of AP-1 adaptor function. Reason: Retain clathrin-cargo adaptor activity, with the authored core explicitly phrased as a contribution by an AP-1 subunit. Gamma ear coat/accessory contacts and gamma/sigma cargo recognition support its role in the assembled adaptor; exact PAINT node placement remains uninspected. No autonomous full cargo-bridging activity or ordinary-qualifier edit is implied. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000512793 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. dictyBase:DDB_G0281957 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. Supporting Evidence: PMID:17360967 the gamma/sigma1 or alpha/sigma2 hemicomplexes bound the dileucine-based motifs of several proteins quite strongly PMID:11451993 the hinge and appendage domains of gamma function in a cooperative manner to recruit and polymerize clathrin PMID:12042876 Structure-based mutational analyses reveal a binding site for the accessory proteins that is composed of conserved basic residues |
| GO:0035646 endosome to melanosome transport | IMP PMID:22511774 BLOC-2, AP-3, and AP-1 proteins function in concert with Rab... | KEEP AS NON CORE | Summary: Retain endosome to melanosome transport as a supported secondary aspect. Reason: AP-1-mediated cargo sorting contributes to melanosome biogenesis; PMID:22511774 gamma-specific perturbation and cargo-distribution results support the existing process assertions. Retain this specialized organelle route as non-core, with no NEW process and no implication that all coat components perform pigment chemistry. Supporting Evidence: PMID:22511774 BLOC-2, AP-3, and AP-1 coimmunoprecipitated with Rab38 and Rab32 from MNT-1 melanocytic cell extracts. |
| GO:0043323 positive regulation of natural killer cell degranulation | IMP PMID:23632890 LAMP1/CD107a is required for efficient perforin delivery to ... | UNDECIDED | Summary: The source-specific positive regulation of natural killer cell degranulation assertion remains unresolved. Reason: The indexed original gamma-silencing Results, Figure 7 and Methods establish perforin retention in MPR-positive transport carriers and reduced target-cell lysis in human YTS cells. They do not expose a gamma-specific granule-release assay. LAMP1 release experiments elsewhere in the paper cannot be reassigned to gamma. Retain UNDECIDED pending the relevant target-specific experiment or supplement, without rejecting the curated IMP assertion. |
| GO:0045954 positive regulation of natural killer cell mediated cytotoxicity | IMP PMID:23632890 LAMP1/CD107a is required for efficient perforin delivery to ... | KEEP AS NON CORE | Summary: Retain positive regulation of natural killer cell mediated cytotoxicity as a supported secondary aspect. Reason: 23632890 indexed original Methods, gamma-silencing Results and Figure 7 explicitly perturb adaptin gamma in human YTS cells and show perforin retention and reduced target-cell lysis. Retain specialized NK-cell effects as non-core. Figure 7 is not a direct gamma degranulation assay; unread supplementary details and original IMP provenance remain distinct. Supporting Evidence: PMID:23632890 Disruption of expression of LAMP1 binding partner, adaptor protein 1 (AP-1) sorting complex, also causes retention of perforin in the transport vesicles and inhibits cytotoxicity |
| GO:0048471 perinuclear region of cytoplasm | IEA GO_REF:0000120 | ACCEPT | Summary: Retain perinuclear region of cytoplasm as part of AP-1 adaptor function. Reason: Perinuclear/Golgi and recycling-endosomal AP-1 pools are directly consistent with HPA and PMID:19841138 human MNT1 localization; retain the target biology while exact mouse-transfer provenance is unresolved. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:P22892 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. ensembl:ENSMUSP00000034171 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. UniProtKB-SubCell:SL-0198 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. Supporting Evidence: PMID:19841138 the clathrin adaptor AP-1 and the kinesin motor KIF13A together create peripheral recycling endosomal subdomains in melanocytes PMID:12773381 Endogenous or ectopically expressed gamma(1)- adaptin localized to both the trans-Golgi network and endosomes. |
| GO:0048488 synaptic vesicle endocytosis | IEA GO_REF:0000107 | UNDECIDED | Summary: The source-specific synaptic vesicle endocytosis assertion remains unresolved. Reason: Exact donor experiment and target-specific presynaptic/synaptic-vesicle function were not recovered. AP-1 specialization alone does not prove these particular neuronal routes, but it also does not refute a conserved neuronal role. Preserve the electronic assertion as uncertain; no donor-count/qualifier inference. Propagation Review Root cause: UNRESOLVED Sources checked: UniProtKB:A0A8I5Y697 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. ensembl:ENSRNOP00000077402 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. |
| GO:0055037 recycling endosome | IDA PMID:19841138 AP-1 and KIF13A coordinate endosomal sorting and positioning... | ACCEPT | Summary: Retain recycling endosome as part of AP-1 adaptor function. Reason: The original PMID:19841138 Results directly localize AP-1 to recycling-endosomal subdomains in human MNT-1 melanocytes. The AP-1 knockdown targets mu1A and its phenotype is not relabeled gamma-specific. This existing human IDA is distinct from other rows with unresolved mouse-transfer provenance; independent gamma1 endosomal localization further corroborates the location. Supporting Evidence: PMID:19841138 the clathrin adaptor AP-1 and the kinesin motor KIF13A together create peripheral recycling endosomal subdomains in melanocytes PMID:12773381 Endogenous or ectopically expressed gamma(1)- adaptin localized to both the trans-Golgi network and endosomes. |
| GO:0060155 platelet dense granule organization | NAS PMID:23247405 Cell type-specific Rab32 and Rab38 cooperate with the ubiqui... | UNDECIDED | Summary: The source-specific platelet dense granule organization assertion remains unresolved. Reason: The actual review paragraph assigns established platelet dense-granule defects to AP-3/BLOC/Rab38 pathways and describes broader application of that machinery as prospective. Broad AP-1 cargo sorting does not independently establish gamma1 participation in platelet dense-granule organization. The source-specific NAS assertion remains UNDECIDED; this is not a claim that gamma1 lacks that role. |
| GO:0090160 Golgi to lysosome transport | IMP PMID:23632890 LAMP1/CD107a is required for efficient perforin delivery to ... | ACCEPT | Summary: Retain Golgi to lysosome transport as part of AP-1 adaptor function. Reason: 23632890 gamma depletion impairs cargo delivery from TGN carriers to lytic granules; together with direct adaptor assembly/cargo recognition this supports the existing transport role. Retain core transport participation without interpreting target lysosomal destination as gamma membrane residence. Supporting Evidence: PMID:23632890 Disruption of expression of LAMP1 binding partner, adaptor protein 1 (AP-1) sorting complex, also causes retention of perforin in the transport vesicles and inhibits cytotoxicity |
| GO:0098793 presynapse | IEA GO_REF:0000107 | UNDECIDED | Summary: The source-specific presynapse assertion remains unresolved. Reason: Exact donor experiment and target-specific presynaptic/synaptic-vesicle function were not recovered. AP-1 specialization alone does not prove these particular neuronal routes, but it also does not refute a conserved neuronal role. Preserve the electronic assertion as uncertain; no donor-count/qualifier inference. Propagation Review Root cause: UNRESOLVED Sources checked: UniProtKB:P22892 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. ensembl:ENSMUSP00000034171 UNRESOLVED The exact source experiment, electronic rule or PAINT ancestral placement was not reconstructed. Independent target evidence is evaluated separately; donor count and target self-inclusion are not evidence of failure. |
| GO:0110010 basolateral protein secretion | NAS PMID:27057418 Role of the epithelial cell-specific clathrin adaptor comple... | KEEP AS NON CORE | Summary: Retain basolateral protein secretion as a supported secondary aspect. Reason: 27057418 reviews AP-1B basolateral recycling/secretion; gamma1 is shared with AP-1A, whereas the epithelial specialization depends on the mu1B-containing complex. Retain specialized epithelial route as non-core, not a unique gamma1 cell-type restriction. Supporting Evidence: PMID:27057418 AP-1B facilitates basolateral sorting from REs. |
| GO:1903232 melanosome assembly | NAS PMID:23247405 Cell type-specific Rab32 and Rab38 cooperate with the ubiqui... | KEEP AS NON CORE | Summary: Retain melanosome assembly as a supported secondary aspect. Reason: AP-1-mediated cargo sorting contributes to melanosome biogenesis; PMID:22511774 gamma-specific perturbation and cargo-distribution results support the existing process assertions. Retain this specialized organelle route as non-core, with no NEW process and no implication that all coat components perform pigment chemistry. Supporting Evidence: PMID:22511774 BLOC-2, AP-3, and AP-1 coimmunoprecipitated with Rab38 and Rab32 from MNT-1 melanocytic cell extracts. |
Loading supporting contentβ¦
Download this section (compressed HTML)Q: Which original target-specific experiment supports the three lysosomal-membrane assertions, as distinct from a transient coat on lysosome-destined carriers?
Q: Which AP1G1-specific release assay supports the NK-cell degranulation assertion, beyond perforin delivery and target-cell lysis?
Q: What experiments support the rat collagen-binding and specialized presynaptic donor annotations, and the exact PAINT placement of the inherited AP-1 activities?
Experiment: Separate cargo recognition from coat recruitment with human gamma/sigma reconstitution and matched gamma hinge/ear mutations, measuring binding, clathrin assembly and membrane recruitment independently.
Loading supporting contentβ¦
Download this section (compressed HTML)Loading supporting contentβ¦
Download this section (compressed HTML)