ARID2, also called BAF200, is a nuclear DNA-binding subunit that specifies the PBAF branch of mammalian SWI/SNF chromatin-remodeling complexes. It supports PBAF assembly and promoter-selective transcriptional regulation while contributing to remodeling powered by the BRG1 ATPase. Its ARID region binds DNA without an established sequence preference. ARID2 also associates with RAD51 and promotes its recruitment during homologous-recombination repair of DNA double-strand breaks. ARID2-containing assemblies with and without BRG1 have been recovered, indicating that its repair interactions cannot be reduced to a single fixed complex composition.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0000776 kinetochore | NAS PMID:11078522 The human SWI/SNF-B chromatin-remodeling complex is related ... | UNDECIDED | Summary: Precise kinetochore localization remains unresolved. Reason: This is a ComplexPortal-assigned NAS (ECO:0005547). BAF180-based PBAF kinetochore staining is a plausible complex-level proxy, but direct ARID2 location and the composition at mitotic kinetochores remain unresolved. The normal cache has Abstract/Discussion only despite its full-text flag; do not infer wrong-gene attribution. |
| GO:0000785 chromatin | NAS PMID:12192000 REST repression of neuronal genes requires components of the... | ACCEPT | Summary: Chromatin association is consistent with established human ARID2 biology. Reason: This is a ComplexPortal-assigned NAS (ECO:0005547). Chromatin association is independently consistent with human ARID2 PBAF membership, DNA binding and transcriptional targeting. The earlier REST-focused NAS citation is not claimed to be a new ARID2-specific experiment. Supporting Evidence: file:human/ARID2/ARID2-notes.md Reciprocal Flag-BAF200 recovery includes BRG1, BAF180, BAF57 and hSNF5, with DNA-mediated association controlled using ethidium bromide. Reactome:R-HSA-9932440 ARID2 is a core component of the base module of the pBAF complex |
| GO:0003677 DNA binding | IBA GO_REF:0000033 | ACCEPT | Summary: The inherited DNA-binding function has direct experimental support. Reason: The inherited DNA-binding assertion agrees with the human domain assay. Target self-inclusion among descendants is legitimate. Do not challenge the PAINT node from donor count; complete tree not reconstructed. Supporting Evidence: PMID:15640446 Analysis of ARID2 in the DNA pull-down assay (Figure 4) indicates that it binds DNA without sequence specificity |
| GO:0003677 DNA binding | IDA PMID:15640446 DNA-binding properties of ARID family proteins. | ACCEPT | Summary: The human ARID2 domain binds DNA without clear sequence preference. Reason: Human HepG2-derived N-terminal ARID2 fragment binds DNA in the original assay without clear sequence preference; targeted Methods/Results read. Supporting Evidence: PMID:15640446 Analysis of ARID2 in the DNA pull-down assay (Figure 4) indicates that it binds DNA without sequence specificity |
| GO:0003677 DNA binding | IEA GO_REF:0000002 | ACCEPT | Summary: ARID-domain mapping agrees with experimental DNA binding. Reason: The ARID-domain mapping agrees with experimental human ARID2 DNA binding; no AT-rich specificity inferred. Supporting Evidence: PMID:15640446 Analysis of ARID2 in the DNA pull-down assay (Figure 4) indicates that it binds DNA without sequence specificity |
| GO:0005515 protein binding | IPI PMID:15985610 PBAF chromatin-remodeling complex requires a novel specifici... | MODIFY | Summary: Refine the demonstrated BRG1 association to ATPase binding. Reason: Original Flag-BAF200 copurification confirms association with the ATPase BRG1. Refine the established IPI to GO:0051117 ATPase binding without claiming an isolated binary interface or catalytic activity of ARID2. Proposed replacements: ATPase binding Supporting Evidence: file:human/ARID2/ARID2-notes.md Reciprocal Flag-BAF200 recovery includes BRG1, BAF180, BAF57 and hSNF5, with DNA-mediated association controlled using ethidium bromide. |
| GO:0005515 protein binding | IPI PMID:15985610 PBAF chromatin-remodeling complex requires a novel specifici... | KEEP AS NON CORE | Summary: Retain the established SMARCB1 association outside the molecular core. Reason: Original Flag-BAF200 copurification confirms hSNF5/SMARCB1 association. This is a supported association within PBAF, retained outside the molecular core; complex membership alone does not establish an additional complex-binding activity. Supporting Evidence: file:human/ARID2/ARID2-notes.md Reciprocal Flag-BAF200 recovery includes BRG1, BAF180, BAF57 and hSNF5, with DNA-mediated association controlled using ethidium bromide. |
| GO:0005515 protein binding | IPI PMID:15985610 PBAF chromatin-remodeling complex requires a novel specifici... | KEEP AS NON CORE | Summary: Retain the established PBRM1 association outside the molecular core. Reason: Original reciprocal PBAF isolation confirms BAF180/PBRM1 association, including ethidium-bromide control. Keep as non-core rather than treating co-purification as a histone-reader activity of ARID2. Supporting Evidence: file:human/ARID2/ARID2-notes.md Reciprocal Flag-BAF200 recovery includes BRG1, BAF180, BAF57 and hSNF5, with DNA-mediated association controlled using ethidium bromide. |
| GO:0005515 protein binding | IPI PMID:15985610 PBAF chromatin-remodeling complex requires a novel specifici... | KEEP AS NON CORE | Summary: Retain the established SMARCE1 association outside the molecular core. Reason: Original Flag-BAF200 copurification confirms BAF57/SMARCE1 association. Keep non-core; DNA binding by the partner does not alone establish a DNA-binding transcription-factor-specific binding activity. Supporting Evidence: file:human/ARID2/ARID2-notes.md Reciprocal Flag-BAF200 recovery includes BRG1, BAF180, BAF57 and hSNF5, with DNA-mediated association controlled using ethidium bromide. |
| GO:0005515 protein binding | IPI PMID:22140357 Repressive LTR nucleosome positioning by the BAF complex is ... | MODIFY | Summary: The source directly supports BAF200 association with the BRG1 ATPase. Reason: Figure 3C of PMID:22140357 directly examines BAF200 among BRG1-associated proteins after BRG1 immunoprecipitation from human J-Lat A2 cells. Refine the generic interaction to ATPase binding because the named partner is SMARCA4/BRG1. This is a complex-associated interaction, not proof of a purified binary interface or ATP hydrolysis by ARID2. The 2015 correction reports image-presentation problems in other specified panels; it does not identify Figure 3C as corrected or retract this result. Retain that quality caution on the reference. Proposed replacements: ATPase binding Supporting Evidence: PMID:22140357 BRG-1 was immunoprecipitated from J-Lat A2 cells transfected with control siRNA or siRNA targeting BAF250 or BAF180, and its associated proteins were examined by SDS-PAGE and Western blotting with antibodies against BRG-1 itself, the BAF or PBAF-specific subunits BAF250a, BAF180 and BAF200, the core subunit INI-1 and GAPDH as control. |
| GO:0005515 protein binding | IPI PMID:24981860 Human-chromatin-related protein interactions identify a deme... | UNDECIDED | Summary: The specific PMID:24981860 interaction remains unresolved. Reason: PMID:24981860 describes tagged human chromatin-protein affinity purifications and mass spectrometry. The accessible abstract does not resolve the ARID2-SMARCB1 observation, and direct publisher access did not recover its experiment. Keep this source-specific assertion unresolved, although independent human PBAF studies establish association of these proteins. |
| GO:0005515 protein binding | IPI PMID:24981860 Human-chromatin-related protein interactions identify a deme... | UNDECIDED | Summary: The specific PMID:24981860 interaction remains unresolved. Reason: The PMID:24981860 AP-MS survey provides the context for this ARID2-SMARCE1 annotation, but its precise bait/prey experiment has not been inspected. The abstract and independent PBAF association do not resolve this particular observation. Its exact source location remains unknown. |
| GO:0005515 protein binding | IPI PMID:30108113 Comprehensive evaluation of coding region point mutations in... | UNDECIDED | Summary: The specific PMID:30108113 interaction remains unresolved. Reason: The original PMID:30108113 interactome Results profile SMARCB1 by both AP-MS and BioID in Flp-In T-REx 293 cells. These methods distinguish copurification from proximity, but the exact ARID2-SMARCB1 record has not been inspected. Retain uncertainty about this source-specific assay, rather than treating the independently known PBAF association as its verification. |
| GO:0005515 protein binding | IPI PMID:31759698 Recurrent SMARCB1 Mutations Reveal a Nucleosome Acidic Patch... | UNDECIDED | Summary: The specific PMID:31759698 interaction remains unresolved. Reason: PMID:31759698 shows that SMARCB1 variants incorporate into mSWI/SNF complexes and assesses assembly by immunoprecipitation, immunoblotting and proteomics. The inspected Results do not resolve the particular ARID2-SMARCB1 observation; ARID2 text mentions concern ChIP-seq data. The relevant image or data record remains unread, so this source-specific pair stays unresolved. |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | UNDECIDED | Summary: The specific PMID:33961781 interaction remains unresolved. Reason: PMID:33961781 maps tagged-protein associations in 293T and HCT116 cells. The entire cached body was read, but it contains Introduction/Discussion rather than the original pair-level Results and Methods. The exact ARID2-SMARCB1 bait/prey context remains unread; independent PBAF association does not identify this experiment. |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | UNDECIDED | Summary: The specific PMID:33961781 interaction remains unresolved. Reason: BioPlex3.0 in PMID:33961781 compares AP-MS interactions between 293T and HCT116 cells. The extracted cache does not expose the ARID2-SMARCE1 pair-level result or its cellular context. Keep the original assertion unresolved pending that record, without assigning it an unverified supplementary-table location. |
| GO:0005515 protein binding | IPI PMID:34591612 A protein interaction landscape of breast cancer. | UNDECIDED | Summary: The specific PMID:34591612 interaction remains unresolved. Reason: PMID:34591612 profiles 40 cancer-associated proteins across three human breast cell lines. Its cached abstract/Discussion establish the AP-MS survey context, but the ARID2-SMARCB1 record, bait and mutant/cell-line conditions have not been inspected. That source-specific uncertainty remains despite independent evidence that the partners associate in PBAF. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | UNDECIDED | Summary: The specific PMID:35271311 interaction remains unresolved. Reason: OpenCell in PMID:35271311 uses endogenous tagging and IP-MS in HEK293T cells. The inspected abstract/Discussion do not identify the specific ARID2-SMARCA4 measurement; shared localization is not a substitute for its interaction result. Keep this row unresolved until its own experimental record can be checked. |
| GO:0005515 protein binding | IPI PMID:40205054 Multimodal cell maps as a foundation for structural and func... | UNDECIDED | Summary: The specific PMID:40205054 interaction remains unresolved. Reason: PMID:40205054 combines U2OS tagged-bait AP-MS with imaging and other measurements. The proteomics acquisition and quality-control Results were inspected, but the exact ARID2-SMARCE1 interaction record and its modality remain unread. Integrated-map context does not settle this source-specific pair. |
| GO:0005634 nucleus | IBA GO_REF:0000033 | ACCEPT | Summary: Nuclear location agrees with human PBAF isolation. Reason: Nuclear localization is supported by independent human nuclear-extract PBAF isolation and curated location, consistent with the inherited assertion; no phylogenetic objection. |
| GO:0005634 nucleus | IEA GO_REF:0000044 | ACCEPT | Summary: Curated nuclear-location mapping is consistent with experiment. Reason: UniProt location mapping is consistent with the human nuclear evidence. |
| GO:0005654 nucleoplasm | IDA GO_REF:0000052 | ACCEPT | Summary: Retain curated nucleoplasmic localization. Reason: Retain curated HPA nucleoplasmic location, supported by human nuclear PBAF biology. No claim to have independently inspected the HPA image. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9932440 | ACCEPT | Summary: The ARID2 assembly reaction supports nucleoplasmic location. Reason: ARID2-containing PBAF assembly step is consistent with established nucleoplasmic localization; complete normal Reactome record read. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9932832 | ACCEPT | Summary: The BRD7 assembly reaction supports nucleoplasmic location. Reason: BRD7 recruitment to the ARID2-containing assembly supports this existing nucleoplasmic location; no BRD7 histone-reader activity is transferred to ARID2. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9932843 | ACCEPT | Summary: The PHF10 assembly reaction supports nucleoplasmic location. Reason: PHF10 recruitment step supports the location of the ARID2-containing PBAF assembly. The cache calls PHF10 a bromodomain protein; that descriptor is not used to infer ARID2 activity. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9933237 | ACCEPT | Summary: The ATPase-module assembly reaction supports nucleoplasmic location. Reason: PBRM1/ATPase assembly step is consistent with nucleoplasmic PBAF. The cache has apparent SMARCA2/3 wording and BRD& title artifacts, neither of which is copied into ARID2 mechanism. |
| GO:0006338 chromatin remodeling | IDA PMID:8895581 Purification and biochemical heterogeneity of the mammalian ... | ACCEPT | Summary: ARID2 contributes to chromatin remodeling as a PBAF subunit. Reason: Retain complex-level chromatin remodeling; independent human ARID2 PBAF evidence supports biological correctness while original GOA IDA/source/qualifier remain intact. Supporting Evidence: PMID:8895581 Complexes containing BRG1 can disrupt nucleosomes Reactome:R-HSA-9932440 ARID2 is a core component of the base module of the pBAF complex |
| GO:0006338 chromatin remodeling | NAS PMID:10078207 Reconstitution of a core chromatin remodeling complex from S... | ACCEPT | Summary: Independent ARID2 evidence supports participation in chromatin remodeling. Reason: This is a ComplexPortal-assigned NAS (ECO:0005547). ARID2 helps specify and stabilize the chromatin-remodeling PBAF complex. The four-subunit minimal reconstitution is not asserted to require ARID2; it establishes a catalytic core, while independent evidence supports ARID2 participation. Supporting Evidence: PMID:8895581 Complexes containing BRG1 can disrupt nucleosomes Reactome:R-HSA-9932440 ARID2 is a core component of the base module of the pBAF complex |
| GO:0006355 regulation of DNA-templated transcription | IEA GO_REF:0000002 | ACCEPT | Summary: ARID2 contributes to transcriptional regulation. Reason: Human ARID2-dependent IFITM1 induction supports the broad transcriptional regulation claim; domain mapping is consistent with it. Supporting Evidence: PMID:15985610 The selectivity by PBAF requires a novel subunit, BAF200, but not the previously described PBAF-specificity subunit, BAF180 (Polybromo). file:human/ARID2/ARID2-notes.md BAF200 depletion reduces BAF180 abundance and prevents normal IFITM1 induction; IFITM3 instead depends on BAF250a. |
| GO:0006357 regulation of transcription by RNA polymerase II | NAS PMID:18809673 BRD7, a novel PBAF-specific SWI/SNF subunit, is required for... | ACCEPT | Summary: PBAF target selection supports regulation of Pol II transcription. Reason: This is a ComplexPortal-assigned NAS (ECO:0005547). Human IFITM1 targeting and PBAF transcriptional regulation independently ground the Pol II claim. BRD7-targeted mouse ESC experiments are corroborating complex evidence, not a fictional ARID2 depletion assay. Supporting Evidence: PMID:15985610 The selectivity by PBAF requires a novel subunit, BAF200, but not the previously described PBAF-specificity subunit, BAF180 (Polybromo). file:human/ARID2/ARID2-notes.md BAF200 depletion reduces BAF180 abundance and prevents normal IFITM1 induction; IFITM3 instead depends on BAF250a. |
| GO:0008285 negative regulation of cell population proliferation | IDA PMID:26169693 miR-208-3p promotes hepatocellular carcinoma cell proliferat... | KEEP AS NON CORE | Summary: Retain contextual inhibition of cell proliferation. Reason: Human HepG2/Hep3B overexpression and knockdown results support contextual inhibition of proliferation; keep outside the core molecular mechanism. Supporting Evidence: PMID:26169693 miR-208-3p down-regulation and ARID2 over-expression elicited similar inhibitory effects on HCC cell proliferation and invasion. file:human/ARID2/ARID2-notes.md Human HepG2/Hep3B experiments show suppression of proliferation and transwell invasion after ARID2 expression, with partial reversal by ARID2 knockdown. |
| GO:0016363 nuclear matrix | NAS PMID:9128241 Components of the human SWI/SNF complex are enriched in acti... | UNDECIDED | Summary: Specific nuclear-matrix localization remains unresolved. Reason: This is a ComplexPortal-assigned NAS (ECO:0005547). Nuclear matrix is a more specific location than nucleus/chromatin. The legacy component-fractionation source has not been fully adjudicated for ARID2 or the relevant assembly; no wrong-gene conclusion. |
| GO:0016514 SWI/SNF complex | IDA PMID:8895581 Purification and biochemical heterogeneity of the mammalian ... | ACCEPT | Summary: Retain SWI/SNF-family complex membership. Reason: The current GO:0016514 definition describes an 8–14-protein SWI/SNF-type complex containing the SNF2/SMARCA4 ATPase or an ortholog and does not exclude mammalian PBAF. Independent human BAF200 purification establishes ARID2 membership in BRG1-containing PBAF, corroborating the broad complex annotation while the historical PMID:8895581 source is preserved. GO:0016586 specifically includes PBAF as a synonym and shares the GO:0070603 parent with GO:0016514; this is not an asserted formal parent-child relation between the two complex terms. Supporting Evidence: file:human/ARID2/ARID2-notes.md Reciprocal Flag-BAF200 recovery includes BRG1, BAF180, BAF57 and hSNF5, with DNA-mediated association controlled using ethidium bromide. Reactome:R-HSA-9932440 ARID2 is a core component of the base module of the pBAF complex |
| GO:0016586 RSC-type complex | NAS PMID:8804307 Diversity and specialization of mammalian SWI/SNF complexes. | ACCEPT | Summary: The RSC-type complex term explicitly includes mammalian PBAF. Reason: This is a ComplexPortal-assigned NAS (ECO:0005547). Official GO:0016586 explicitly includes mammalian PBAF. Independent human ARID2 purification establishes this membership; the original NAS reference is preserved. Supporting Evidence: file:human/ARID2/ARID2-notes.md Reciprocal Flag-BAF200 recovery includes BRG1, BAF180, BAF57 and hSNF5, with DNA-mediated association controlled using ethidium bromide. Reactome:R-HSA-9932440 ARID2 is a core component of the base module of the pBAF complex |
| GO:0030071 regulation of mitotic metaphase/anaphase transition | NAS PMID:25066234 Requirement for PBAF in transcriptional repression and repai... | UNDECIDED | Summary: A specific mitotic-transition role remains unresolved. Reason: This is a ComplexPortal-assigned NAS (ECO:0005547). DSB repair and transcriptional silencing do not by themselves establish regulation of the metaphase/anaphase transition by ARID2. This precise process remains unresolved, not contradicted by a title. |
| GO:0030336 negative regulation of cell migration | IDA PMID:26169693 miR-208-3p promotes hepatocellular carcinoma cell proliferat... | KEEP AS NON CORE | Summary: Retain contextual inhibition of migration/invasion. Reason: Human cell transwell invasion experiments support the curator’s contextual migration-regulation assertion; no motility machinery or general migration function inferred. Supporting Evidence: PMID:26169693 miR-208-3p down-regulation and ARID2 over-expression elicited similar inhibitory effects on HCC cell proliferation and invasion. file:human/ARID2/ARID2-notes.md Human HepG2/Hep3B experiments show suppression of proliferation and transwell invasion after ARID2 expression, with partial reversal by ARID2 knockdown. |
| GO:0045582 positive regulation of T cell differentiation | NAS PMID:12110891 Reciprocal regulation of CD4/CD8 expression by SWI/SNF-like ... | UNDECIDED | Summary: Specific T-cell differentiation involvement remains unresolved. Reason: This is a ComplexPortal-assigned NAS (ECO:0005547). T-cell lineage bifurcation involves tested BRG1/BAF57, but direct ARID2 or uniquely PBAF-dependent participation is not established by the inspected abstract. Leave the precise existing claim unresolved. |
| GO:0045597 positive regulation of cell differentiation | NAS PMID:11790558 SWI/SNF chromatin remodeling and cancer. | KEEP AS NON CORE | Summary: Retain broad differentiation effects outside the molecular core. Reason: This is a ComplexPortal-assigned NAS (ECO:0005547). Independent mouse ARID2 depletion and promoter studies (PMID22184115) support a conserved role in promoting differentiation. Retain as contextual, with the species boundary and original NAS citation explicit; the normal source cache has been recovered. Supporting Evidence: PMID:22184115 This analysis identifies a critical role for ARID2-containing complexes in promoting osteoblast differentiation file:human/ARID2/ARID2-notes.md ARID2 depletion impairs differentiation and promoter recruitment of the transcription machinery in a locus-dependent manner. |
| GO:0045663 positive regulation of myoblast differentiation | NAS PMID:15985610 PBAF chromatin-remodeling complex requires a novel specifici... | UNDECIDED | Summary: Specific myoblast differentiation involvement remains unresolved. Reason: This is a ComplexPortal-assigned NAS (ECO:0005547). Human IFITM1 regulation, mouse osteoblast differentiation and general PBAF biology do not settle the specific myoblast differentiation assertion. Preserve as unresolved. |
| GO:0070316 regulation of G0 to G1 transition | NAS PMID:11790558 SWI/SNF chromatin remodeling and cancer. | UNDECIDED | Summary: Specific G0/G1 regulation remains unresolved. Reason: This is a ComplexPortal-assigned NAS (ECO:0005547). General SWI/SNF growth control does not establish ARID2-specific G0/G1 regulation; original NAS review not fully available. |
| GO:0140658 ATP-dependent chromatin remodeler activity | IDA PMID:8895581 Purification and biochemical heterogeneity of the mammalian ... | ACCEPT | Summary: ARID2 contributes to the ATP-dependent activity of PBAF. Reason: Retain the original complex-level ATP-driven remodeling assertion. ARID2 contributes to the PBAF activity; BRG1 supplies ATP hydrolysis. Core uses contributes_to_molecular_function, original qualifier unchanged. Supporting Evidence: PMID:8895581 Complexes containing BRG1 can disrupt nucleosomes Reactome:R-HSA-9932440 ARID2 is a core component of the base module of the pBAF complex |
| GO:1905168 positive regulation of double-strand break repair via homologous recombination | IMP PMID:28381560 The chromatin-remodeling subunit Baf200 promotes homology-di... | ACCEPT | Summary: ARID2 promotes RAD51-dependent homologous-recombination repair. Reason: Human ARID2 depletion impairs RAD51 recruitment and homologous-recombination repair; original target-focused abstract and indexed Results support this existing process. Supporting Evidence: PMID:28381560 Baf200 plays an important function by facilitating homologous recombination-dependent processes, such as recruitment of Rad51 (a key component of homologous recombination) to DSBs, homology-directed repair, and cell survival after DNA damage. file:human/ARID2/ARID2-notes.md Human U2OS experiments support ARID2 association with RAD51 and its efficient recruitment to homologous-recombination sites. |
| GO:2000045 regulation of G1/S transition of mitotic cell cycle | NAS PMID:10778858 Exit from G1 and S phase of the cell cycle is regulated by r... | UNDECIDED | Summary: Specific G1/S regulation remains unresolved. Reason: This is a ComplexPortal-assigned NAS (ECO:0005547). Rb/SWI/SNF control of G1/S is not automatically an ARID2-specific function. The relevant full original experiment remains unread; preserve uncertainty without removal. |
| GO:2000781 positive regulation of double-strand break repair | NAS PMID:25066234 Requirement for PBAF in transcriptional repression and repai... | ACCEPT | Summary: Independent ARID2 evidence supports positive regulation of double-strand-break repair. Reason: This is a ComplexPortal-assigned NAS (ECO:0005547). Independent human ARID2 homologous-recombination evidence supports the broader positive DSB repair assertion. Do not claim the original BAF180-focused study is an ARID2-specific assay. Supporting Evidence: PMID:28381560 Baf200 plays an important function by facilitating homologous recombination-dependent processes, such as recruitment of Rad51 (a key component of homologous recombination) to DSBs, homology-directed repair, and cell survival after DNA damage. file:human/ARID2/ARID2-notes.md Human U2OS experiments support ARID2 association with RAD51 and its efficient recruitment to homologous-recombination sites. |
| GO:2000819 regulation of nucleotide-excision repair | NAS PMID:12215535 The SWI/SNF chromatin-remodeling factor stimulates repair by... | UNDECIDED | Summary: Specific nucleotide-excision-repair regulation remains unresolved. Reason: This is a ComplexPortal-assigned NAS (ECO:0005547). Broad repair function does not establish nucleotide-excision repair. The original purified-complex experiment has not been resolved for ARID2; leave the specific assertion unresolved. |
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Download this section (compressed HTML)Q: Which ARID2-containing assembly recruits RAD51 at each repair stage, and which effects require BRG1 catalytic activity rather than its structural presence?
Experiment: Test endogenous ARID2 interface variants with preserved abundance and PBAF assembly, measuring RAD51 association, bulk versus site-specific recruitment, and homologous-recombination repair alongside BRG1 ATPase controls.
Hypothesis: ARID2 RAD51 recruitment and PBAF assembly can be separated genetically.
Type: Separation-of-function genetics and chromatin recruitment assays
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