ARL13B is a membrane-associated regulatory GTPase enriched in primary cilia. It promotes guanine-nucleotide exchange on ARL3 and cooperates with ARL2BP/BART to regulate the active ARL3 pool involved in ciliary protein trafficking. ARL13B also binds tubulin through its GTPase domain and contributes to the organization and retention of the ciliary membrane relative to the axoneme. Nucleotide-dependent exocyst association and additional trafficking interactions connect it to ciliary protein organization. Its own GTP hydrolysis and its exchange-factor activity are distinct biochemical properties. ARL13B-dependent ciliary organization influences developmental signaling, and pathogenic variants cause Joubert syndrome.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0001822 kidney development | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Retain the kidney-development phenotype as tissue context. Reason: Retain the existing mouse-to-human kidney-development context. Conditional mouse renal loss and ciliary defects in PMID:26582389 support a tissue-level consequence of disrupted ciliary function, rather than a separate kidney-specific molecular activity. |
| GO:0003924 GTPase activity | IEA GO_REF:0000002 | ACCEPT | Summary: Stimulated GTP hydrolysis is supported, with weak intrinsic activity unresolved. Reason: Later human and murine biochemical measurements in PMID:28487361 support stimulated GTP hydrolysis, with protein-dependence and negative controls. Weak intrinsic turnover remains potentially confounded by contaminants and the bovine-brain stimulating factor was not identified. The older statement that hydrolysis was undetected is insufficient to reject the electronic function; strong autonomous human hydrolysis is not asserted. Supporting Evidence: PMID:28487361 We found the GAP-dependent GTP hydrolysis was Arl13b-dependent, indicating that it is a GAP and not a nonspecific nucleotidase. |
| GO:0005515 protein binding | IPI PMID:20643351 Pitchfork regulates primary cilia disassembly and left-right... | KEEP AS NON CORE | Summary: Human PIFO coassociation is demonstrated in human HEK293T cells. Reason: The actual PMID:20643351 Results describe mouse and human tagged PIFO pulling down endogenous ARL13B from human HEK293T cells. Retain each original partner identity and the co-complex association. These experiments do not define a distinct ARL13B catalytic activity or demonstrate purified pair binding. Supporting Evidence: PMID:20643351 We precipitated endogenous proteins using immobilized mouse and human SF-TAP-tagged PIFO in ciliated and cilia-disassembling HEK293T cells. This pull-down analysis revealed that the early endosome marker Rab6, the late endosome marker Rab8, the cilium localized small GTPase Arl13b (Caspary et al., 2007), the PCM protein Ξ³-Tubulin, the centriolar protein Centrin, the MT protein Ξ²-Tubulin, and the motor protein Kif3a, all physically interact with Pifo/PIFO in cell culture (Figure 5G and Figures S5EβS5H). |
| GO:0005515 protein binding | IPI PMID:20643351 Pitchfork regulates primary cilia disassembly and left-right... | KEEP AS NON CORE | Summary: Mouse PIFO pulls down endogenous human ARL13B. Reason: The actual PMID:20643351 Results describe mouse and human tagged PIFO pulling down endogenous ARL13B from human HEK293T cells. Retain each original partner identity and the co-complex association. These experiments do not define a distinct ARL13B catalytic activity or demonstrate purified pair binding. Supporting Evidence: PMID:20643351 We precipitated endogenous proteins using immobilized mouse and human SF-TAP-tagged PIFO in ciliated and cilia-disassembling HEK293T cells. This pull-down analysis revealed that the early endosome marker Rab6, the late endosome marker Rab8, the cilium localized small GTPase Arl13b (Caspary et al., 2007), the PCM protein Ξ³-Tubulin, the centriolar protein Centrin, the MT protein Ξ²-Tubulin, and the motor protein Kif3a, all physically interact with Pifo/PIFO in cell culture (Figure 5G and Figures S5EβS5H). |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:A2RU14 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:A2RU14. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:A5D903 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:A5D903. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:A5PKU2 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:A5PKU2. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:A6NDP7 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:A6NDP7. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:O14493 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:O14493. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:O14523 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:O14523. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:O14569 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:O14569. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:O14653 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:O14653. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:O14735 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:O14735. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:O14798 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:O14798. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:O15400 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:O15400. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:O60725 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:O60725. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:O60883 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:O60883. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:O75355-2 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:O75355-2. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:O75379 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:O75379. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:O75425 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:O75425. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:O95214 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:O95214. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:O95393 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:O95393. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:P02786 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:P02786. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:P09466 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:P09466. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:P09601 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:P09601. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:P0DN84 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:P0DN84. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:P11215 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:P11215. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:P13236 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:P13236. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:P16150 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:P16150. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:P17152 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:P17152. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:P19397 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:P19397. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:P22732 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:P22732. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:P23763-3 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:P23763-3. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:P26678 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:P26678. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:P29033 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:P29033. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:P29034 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:P29034. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:P30519 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:P30519. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:P37268 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:P37268. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:P43378 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:P43378. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:P48509 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:P48509. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:P54849 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:P54849. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:P56748 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:P56748. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:P60201-2 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:P60201-2. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:P63027 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:P63027. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:P63252 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:P63252. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:P78382 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:P78382. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:P78383 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:P78383. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q01453 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q01453. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q05329 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q05329. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q0VAQ4 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q0VAQ4. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q12983 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q12983. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q13021 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q13021. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q13113 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q13113. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q14318 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q14318. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q14508 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q14508. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q14656 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q14656. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q15125 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q15125. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q15546 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q15546. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q15836 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q15836. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q16617 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q16617. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q16623 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q16623. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q16853 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q16853. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q4VAQ0 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q4VAQ0. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q59EV6 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q59EV6. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q5BJF2 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q5BJF2. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q5BJH2-2 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q5BJH2-2. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q5QGT7 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q5QGT7. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q5TGU0 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q5TGU0. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q5VZY2 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q5VZY2. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q6PI78 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q6PI78. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q6PL45-2 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q6PL45-2. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q6UX27-3 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q6UX27-3. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q6UXB4 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q6UXB4. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q71RG4 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q71RG4. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q86W74-2 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q86W74-2. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q8IX05 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q8IX05. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q8IXM6 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q8IXM6. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q8N138 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q8N138. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q8N5M9 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q8N5M9. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q8N661 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q8N661. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q8N6G5 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q8N6G5. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q8NBD8 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q8NBD8. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q8NHW4 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q8NHW4. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q8NHY0 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q8NHY0. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q8TBM7 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q8TBM7. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q8WVX3-2 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q8WVX3-2. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q8WWP7 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q8WWP7. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q8WWT9 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q8WWT9. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q8WZ59 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q8WZ59. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q92685 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q92685. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q969K7 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q969K7. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q969L2 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q969L2. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q96CM8 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q96CM8. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q96DR8 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q96DR8. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q96E93 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q96E93. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q96EC8 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q96EC8. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q96F15 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q96F15. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q96FZ5 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q96FZ5. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q96GM1 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q96GM1. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q96IV6 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q96IV6. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q96MV8 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q96MV8. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q96MX0 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q96MX0. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q96PS8 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q96PS8. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q9BSE2 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q9BSE2. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q9BTD3 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q9BTD3. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q9BTV4 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q9BTV4. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q9BWH2 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q9BWH2. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q9BXN2-6 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q9BXN2-6. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q9BXR6 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q9BXR6. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q9BZW4 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q9BZW4. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q9C0I4 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q9C0I4. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q9H0Q3 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q9H0Q3. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q9H2S6-2 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q9H2S6-2. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q9NP94 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q9NP94. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q9NSC5 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q9NSC5. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q9NTJ5 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q9NTJ5. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q9NTN9-2 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q9NTN9-2. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q9NVC3 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q9NVC3. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q9NVV5-2 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q9NVV5-2. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q9NWW5 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q9NWW5. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q9NZ01 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q9NZ01. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q9NZG7 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q9NZG7. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q9P0B6 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q9P0B6. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q9P0S3 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q9P0S3. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q9P0S9 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q9P0S9. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q9UBY5 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q9UBY5. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q9UHE5 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q9UHE5. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q9UHJ9-5 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q9UHJ9-5. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q9UPZ6 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q9UPZ6. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | UNDECIDED | Summary: The human binary-interactome record for ARL13B and UniProtKB:Q9Y6I9 remains source-limited. Reason: The human binary-interactome annotation names UniProtKB:Q9Y6I9. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:32541000 Kir2.1 Interactome Mapping Uncovers PKP4 as a Modulator of t... | UNDECIDED | Summary: The Kir2.1-interactome record for ARL13B and UniProtKB:P63252 remains source-limited. Reason: The Kir2.1-interactome annotation names UniProtKB:P63252. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005515 protein binding | IPI PMID:40355756 The solute carrier superfamily interactome. | UNDECIDED | Summary: The solute-carrier-interactome record for ARL13B and UniProtKB:P22732 remains source-limited. Reason: The solute-carrier-interactome annotation names UniProtKB:P22732. Its exact pair-specific experiment, construct and controls were not independently adjudicated. Preserve the source and partner isoform where present; the available article-level information does not resolve a more specific activity for this interaction. |
| GO:0005525 GTP binding | IEA GO_REF:0000002 | ACCEPT | Summary: Mammalian ARL13B binds guanine nucleotides. Reason: Guanine-nucleotide binding is directly supported by the original domain/Joubert studies and later solution-based mammalian biochemistry. Assay limitations in older filter-trapping experiments do not erase the corroborating binding measurements. Supporting Evidence: PMID:18554500 Arl13b binds guanine nucleotides and specifically localizes to cilia. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9641096 | KEEP AS NON CORE | Summary: Retain the modeled cytosolic misfolded ARL13B pool as a non-core location. Reason: The inspected Reactome graph explicitly places human Q3SXY8 as misfolded ARL13B (R-HSA-9640110, cytosol), within misfolded ciliary proteins (9640104) and the misfolded-protein set (9641091). That set is an input and a component of the output PRKN complex (9641099). This supports the existing TAS compartment assertion for a disposal substrate; it does not establish a constitutive cytosolic activity or make ARL13B a ubiquitination enzyme. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9641109 | KEEP AS NON CORE | Summary: Retain the modeled cytosolic polyubiquitinated ARL13B disposal pool. Reason: The inspected dissociation graph releases the cytosolic polyubiquitinated-protein set (R-HSA-9641120) from complex 9641113. Its ciliary-protein subset (9641108) explicitly includes polyubiquitinated human ARL13B (9641129, Q3SXY8, cytosol). Retain this source-defined location as non-core, with the modeled disposal state explicit; the graph is not a direct ARL13B localization experiment. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9641111 | KEEP AS NON CORE | Summary: Retain the cytosolic location of ARL13B as the modeled ubiquitination substrate. Reason: The inspected input complex R-HSA-9641099 contains misfolded proteins (9641091), including ciliary proteins (9640104) and human ARL13B (9640110, Q3SXY8, cytosol). Thus the target and compartment are explicit in this Reactome event. The non-core location is retained without assigning Parkin-mediated ubiquitin transfer to its ARL13B substrate. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9641127 | KEEP AS NON CORE | Summary: Retain the modeled cytosolic polyubiquitinated ARL13B pool as non-core. Reason: The inspected polymerization event produces cytosolic complex R-HSA-9641113, whose polyubiquitinated-protein component 9641120 includes ciliary subset 9641108 and human ARL13B 9641129 (Q3SXY8, cytosol). This resolves target membership in the existing TAS assertion. It describes a protein-disposal state, without establishing that ARL13B catalyzes ubiquitin-chain assembly. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9646348 | KEEP AS NON CORE | Summary: Retain cytosolic ARL13B disposal cargo as a non-core modeled location. Reason: The inspected event joins dynein to the cytosolic HDAC6/polyubiquitinated-protein complex. The ARL13B entity page traces human Q3SXY8 (R-HSA-9641129) through ciliary subset 9641108 and protein set 9641120 into both input and output complexes. This supports the compartment assertion for cargo; it does not confer motor or HDAC6 activity on ARL13B. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9646679 | KEEP AS NON CORE | Summary: Retain the cytosol label on the modeled ARL13B cargo within an aggresome complex. Reason: The event-level compartment is the microtubule organizing center, while its nested HDAC6/polyubiquitinated-protein component and human ARL13B entity R-HSA-9641129 (Q3SXY8) retain explicit cytosol labels. The inspected ARL13B graph traces that entity through the input cargo complex and vimentin-containing output. Retain the TAS location as a non-core model-derived disposal context, rather than mistaking the event compartment for every constituent label or inferring a constitutive cytosolic activity. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9646685 | KEEP AS NON CORE | Summary: Retain cytosolic ARL13B in the modeled aggresome-disassembly output. Reason: The inspected output includes a cytosolic vimentin/HDAC6/polyubiquitinated-protein complex. Its protein set R-HSA-9641120 contains ciliary subset 9641108 and polyubiquitinated human ARL13B 9641129 (Q3SXY8, cytosol), as traced on the ARL13B entity page. Retain this non-core disposal location without treating ARL13B as an autophagy effector or claiming direct target-specific imaging. |
| GO:0005856 cytoskeleton | IEA GO_REF:0000044 | ACCEPT | Summary: Cytoskeletal association is independently supported. Reason: Retain the broad cytoskeletal association, supported by the actual actin-associated trafficking observations in PMID:23223633 and direct human-ARL13B/tubulin assays in PMID:29592971. Actin co-IP is not treated as proof of a purified direct interaction. Supporting Evidence: PMID:29592971 To distinguish between a direct or indirect association of ARL13B with tubulin, we isolated recombinant GST-tagged human ARL13B from E. coli and incubated it in the presence of purified bovine brain tubulin. Incubation of the GST protein tag with tubulins was used as control. Glutathione (GSH) sepharose beads were added to each reaction mix to purify GST-ARL13B or control GST, and their interacting proteins were analyzed by western blotting. Tubulin was found in the eluate from the beads loaded with GST-ARL13B, but not in that from control GST ( Fig.Β 2 A and B, lanes βGST-ARL13B aa 1-428β and βGSTβ), thus indicating that ARL13B can directly interact with tubulin dimers. |
| GO:0005886 plasma membrane | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: A nonciliary plasma-membrane pool is reported in human HeLa cells. Reason: Actual PMID:23223633 Results show tagged ARL13B at the plasma membrane in human HeLa cells and distinguish this nonciliary trafficking context. Retain this secondary location separately from its principal ciliary membrane activity; construct-species Methods still require checking. Supporting Evidence: file:human/ARL13B/ARL13B-uniprot.txt ECO:0000269|PubMed:38219074}. Cell membrane CC {ECO:0000269|PubMed:23223633}. Cytoplasm, cytoskeleton |
| GO:0005929 cilium | IDA GO_REF:0000052 | ACCEPT | Summary: Independent mammalian observations corroborate the HPA cilium assignment. Reason: The original HPA cilium image was not independently inspected. Human RPE1 ARL13B-marker experiments, direct ARL13B localization studies and the curated UniProt ciliary-membrane statement corroborate the location. Retain this experimental assertion without claiming an image-level audit. Supporting Evidence: file:human/ARL13B/ARL13B-uniprot.txt CC -!- SUBCELLULAR LOCATION: Cell projection, cilium membrane CC {ECO:0000269|PubMed:18554500, ECO:0000269|PubMed:24120134}; Lipid- CC anchor {ECO:0000269|PubMed:18554500, ECO:0000269|PubMed:24120134}. |
| GO:0005929 cilium | IDA PMID:24421332 The CP110-interacting proteins Talpid3 and Cep290 play overl... | ACCEPT | Summary: The Talpid3 study explicitly uses ARL13B as a human RPE1 cilium marker. Reason: The actual PMID:24421332 Results explicitly use ARL13B among four ciliary markers in serum-starved human RPE1 cells. This supports the existing location even though the study foregrounds Talpid3. Supplementary images and complete Methods were not inspected; no Talpid3 molecular activity is assigned to ARL13B. Supporting Evidence: PMID:24421332 When we acutely ablated Talpid3 using several distinct siRNAs in RPE1 cells and induced cilia formation by serum starvation, we found a significant reduction in primary cilia assembly using four canonical markers, glutamylated tubulin (GT335), detyrosinated tubulin, IFT88, and Arl13b (Fig. S2, B and C; and unpublished data). |
| GO:0005929 cilium | IDA PMID:27623382 A Conserved Role for Girdin in Basal Body Positioning and Ci... | ACCEPT | Summary: The Girdin-source cilium assertion is retained with independent corroboration. Reason: The original Girdin-source microscopy was not re-inspected beyond the cached abstract. Independent human ARL13B localization and biochemical studies corroborate the cilium location, so the positive experimental assertion is retained without claiming a complete audit of that imaging experiment. Supporting Evidence: file:human/ARL13B/ARL13B-uniprot.txt CC -!- SUBCELLULAR LOCATION: Cell projection, cilium membrane CC {ECO:0000269|PubMed:18554500, ECO:0000269|PubMed:24120134}; Lipid- CC anchor {ECO:0000269|PubMed:18554500, ECO:0000269|PubMed:24120134}. |
| GO:0005929 cilium | IEA GO_REF:0000120 | ACCEPT | Summary: Electronic cilium localization agrees with direct mammalian observations. Reason: The combined electronic cilium assignment agrees with independent mammalian ARL13B imaging and the curated ciliary-membrane location. The individual ARBA and orthology rule derivations were not reconstructed; the location is corroborated by direct biology. Supporting Evidence: file:human/ARL13B/ARL13B-uniprot.txt CC -!- SUBCELLULAR LOCATION: Cell projection, cilium membrane CC {ECO:0000269|PubMed:18554500, ECO:0000269|PubMed:24120134}; Lipid- CC anchor {ECO:0000269|PubMed:18554500, ECO:0000269|PubMed:24120134}. |
| GO:0005929 cilium | ISS GO_REF:0000024 | ACCEPT | Summary: The transferred cilium location is corroborated by human experiments. Reason: The curator-transferred cilium location agrees with direct human RPE1 ARL13B-marker observations and independent mammalian localization studies. The underlying mouse experiment was not re-audited; human corroboration supports retaining its original transfer provenance. Supporting Evidence: file:human/ARL13B/ARL13B-uniprot.txt CC -!- SUBCELLULAR LOCATION: Cell projection, cilium membrane CC {ECO:0000269|PubMed:18554500, ECO:0000269|PubMed:24120134}; Lipid- CC anchor {ECO:0000269|PubMed:18554500, ECO:0000269|PubMed:24120134}. |
| GO:0005929 cilium | TAS Reactome:R-HSA-5624953 | ACCEPT | Summary: The ARL13BβINPP5E model places an independently supported pool in cilia. Reason: The ciliary location in the ARL13B/INPP5E trafficking model agrees with independent human localization and the original targeting experiments. Retaining location does not endorse every proposed release or activation step in the complete Reactome model. Supporting Evidence: file:human/ARL13B/ARL13B-uniprot.txt CC -!- SUBCELLULAR LOCATION: Cell projection, cilium membrane CC {ECO:0000269|PubMed:18554500, ECO:0000269|PubMed:24120134}; Lipid- CC anchor {ECO:0000269|PubMed:18554500, ECO:0000269|PubMed:24120134}. |
| GO:0005929 cilium | TAS Reactome:R-HSA-5638012 | ACCEPT | Summary: Cilium localization is supported independently of the modeled release step. Reason: The ciliary location in the ARL13B/INPP5E trafficking model agrees with independent human localization and the original targeting experiments. Retaining location does not endorse every proposed release or activation step in the complete Reactome model. Supporting Evidence: file:human/ARL13B/ARL13B-uniprot.txt CC -!- SUBCELLULAR LOCATION: Cell projection, cilium membrane CC {ECO:0000269|PubMed:18554500, ECO:0000269|PubMed:24120134}; Lipid- CC anchor {ECO:0000269|PubMed:18554500, ECO:0000269|PubMed:24120134}. |
| GO:0005930 axoneme | IEA GO_REF:0000107 | ACCEPT | Summary: Axonemal association is corroborated by binding and ciliary-retention experiments. Reason: The transferred axonemal association is corroborated by human ARL13B fragments binding isolated algal axonemes and by G-domain-dependent retention in mouse cilia in PMID:29592971. ARL13B remains a membrane-associated regulatory protein; no tubulin-polymerase activity or integral doublet-subunit identity is inferred. Supporting Evidence: PMID:29592971 To test whether the G-domain of ARL13B is required to anchor ARL13B to the ciliary axoneme, we performed in vitro pull-down of GST-ARL13B fragments by using demembranated axonemes purified from isolated Chlamydomonas flagella ( Alper et al., 2013 ). The assay showed that both full-length ARL13B and its G-domain can bind isolated axonemes, whereas the coil-coil domain of ARL13B does not ( Fig.Β 6 A). |
| GO:0007224 smoothened signaling pathway | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Retain the inherited Smoothened-signaling context. Reason: Retain the established orthology-supported Smoothened-signaling context as a consequence of ciliary protein organization. The receptor-distribution experiments do not establish that ARL13B itself is the receptor or a pathway-specific kinase, and the molecular functions are described separately. |
| GO:0007224 smoothened signaling pathway | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Retain the curator-transferred Smoothened-signaling role as context. Reason: Retain the established orthology-supported Smoothened-signaling context as a consequence of ciliary protein organization. The receptor-distribution experiments do not establish that ARL13B itself is the receptor or a pathway-specific kinase, and the molecular functions are described separately. |
| GO:0015630 microtubule cytoskeleton | IDA GO_REF:0000052 | ACCEPT | Summary: Direct tubulin and axoneme association corroborates the broad cytoskeletal site. Reason: Direct tubulin and isolated-axoneme binding, together with ciliary retention experiments, corroborate the broad microtubule-cytoskeleton location. The individual HPA image was not inspected; this does not recast the assay as direct proof of a motor or polymerase activity. Supporting Evidence: PMID:29592971 To distinguish between a direct or indirect association of ARL13B with tubulin, we isolated recombinant GST-tagged human ARL13B from E. coli and incubated it in the presence of purified bovine brain tubulin. Incubation of the GST protein tag with tubulins was used as control. Glutathione (GSH) sepharose beads were added to each reaction mix to purify GST-ARL13B or control GST, and their interacting proteins were analyzed by western blotting. Tubulin was found in the eluate from the beads loaded with GST-ARL13B, but not in that from control GST ( Fig.Β 2 A and B, lanes βGST-ARL13B aa 1-428β and βGSTβ), thus indicating that ARL13B can directly interact with tubulin dimers. |
| GO:0021532 neural tube patterning | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Retain the electronic neural-patterning context. Reason: Retain the curated mouse-to-human neural-tube patterning context. Ciliary signaling can influence developmental patterning, while this tissue outcome is distinct from the measured ARL13B molecular activities. The underlying mouse experiments were not independently reread. |
| GO:0021532 neural tube patterning | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Retain the curator-transferred neural-patterning context. Reason: Retain the curated mouse-to-human neural-tube patterning context. Ciliary signaling can influence developmental patterning, while this tissue outcome is distinct from the measured ARL13B molecular activities. The underlying mouse experiments were not independently reread. |
| GO:0021830 interneuron migration from the subpallium to the cortex | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Retain the electronic interneuron-migration context. Reason: Retain the curated ortholog-supported interneuron migration context, also represented in the normal UniProt functional synthesis. This is a developmental consequence rather than a separate migration motor activity. The original mouse tissue experiments were not independently reread. |
| GO:0021830 interneuron migration from the subpallium to the cortex | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Retain the curator-transferred interneuron-migration context. Reason: Retain the curated ortholog-supported interneuron migration context, also represented in the normal UniProt functional synthesis. This is a developmental consequence rather than a separate migration motor activity. The original mouse tissue experiments were not independently reread. |
| GO:0021943 formation of radial glial scaffolds | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Retain the radial-glial developmental phenotype. Reason: Retain the curated ortholog-supported radial-glial scaffold phenotype as developmental context. The anatomical scaffold is not evidence that ARL13B is a protein-complex scaffold. Underlying mouse developmental experiments were not independently reread. |
| GO:0021943 formation of radial glial scaffolds | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Retain the curator-transferred radial-glial phenotype. Reason: Retain the curated ortholog-supported radial-glial scaffold phenotype as developmental context. The anatomical scaffold is not evidence that ARL13B is a protein-complex scaffold. Underlying mouse developmental experiments were not independently reread. |
| GO:0030742 GTP-dependent protein binding | IEA GO_REF:0000107 | ACCEPT | Summary: Nucleotide-sensitive exocyst association supports GTP-dependent protein binding. Reason: PMID:26582389 directly reports nucleotide-sensitive exocyst association, including human-cell co-IP and purified murine-protein experiments. This supports the transferred GTP-dependent binding function without treating every co-IP partner as a direct contact or ARL13B as an exocyst subunit. Supporting Evidence: PMID:26582389 Furthermore, we immunoprecipitated Arl13b from nonciliated HeLa cells and found that Sec8 and Sec5 are also enriched in the precipitates in a GTPΞ³S-sensitive manner, indicating that the interaction is conserved and not dependent upon the presence of cilia (Supplemental Figure S1). |
| GO:0031514 motile cilium | IBA GO_REF:0000033 | UNDECIDED | Summary: The motile-cilium activity-site ancestry remains unresolved. Reason: The exact motile-cilium activity evidence underlying the PAINT ancestral-node placement was not inspected. Primary-cilium evidence alone cannot settle this activity site. The PAINT assertion is a phylogenetic judgment; its short donor list is not treated as weak evidence or a reason for removal. |
| GO:0031514 motile cilium | IEA GO_REF:0000107 | UNDECIDED | Summary: The exact transferred motile-cilium localization evidence remains unread. Reason: The source-specific motile-cilium localization experiment underlying this mouse-to-human transfer was not inspected. Primary-cilium evidence alone cannot settle this location. Preserve the curator assertion pending relevant site-specific evidence, without treating it as a PAINT annotation. |
| GO:0035869 ciliary transition zone | IDA GO_REF:0000052 | UNDECIDED | Summary: Transition-zone assignment requires the original HPA image and context. Reason: The HPA transition-zone image and marker-resolution evidence were not inspected. The curated subciliary exclusion note in UniProt motivates checking the exact cell type and assay, rather than overruling an experimental annotation from a general localization summary. |
| GO:0045171 intercellular bridge | IDA GO_REF:0000052 | UNDECIDED | Summary: The original intercellular-bridge image remains uninspected. Reason: The original HPA intercellular-bridge image was not inspected and the reviewed primary texts do not resolve that site. Preserve the experimental assertion without inventing a cytokinesis mechanism. |
| GO:0060170 ciliary membrane | IBA GO_REF:0000033 | ACCEPT | Summary: Independent biology supports the ancestral ciliary-membrane activity site. Reason: Ciliary membrane localization is central to ARL13B function and is supported by independent mammalian localization, domain and trafficking experiments. Retain each inherited source, including PAINT, without claiming a new family-tree reconstruction. Supporting Evidence: file:human/ARL13B/ARL13B-uniprot.txt CC -!- SUBCELLULAR LOCATION: Cell projection, cilium membrane CC {ECO:0000269|PubMed:18554500, ECO:0000269|PubMed:24120134}; Lipid- CC anchor {ECO:0000269|PubMed:18554500, ECO:0000269|PubMed:24120134}. |
| GO:0060170 ciliary membrane | IEA GO_REF:0000120 | ACCEPT | Summary: Electronic ciliary-membrane localization is independently corroborated. Reason: Independent mammalian domain/localization and trafficking experiments support ciliary-membrane localization. Preserve the original electronic mapping and orthology provenance. This is biological corroboration, not a reconstruction of every transfer rule. Supporting Evidence: file:human/ARL13B/ARL13B-uniprot.txt CC -!- SUBCELLULAR LOCATION: Cell projection, cilium membrane CC {ECO:0000269|PubMed:18554500, ECO:0000269|PubMed:24120134}; Lipid- CC anchor {ECO:0000269|PubMed:18554500, ECO:0000269|PubMed:24120134}. |
| GO:0060170 ciliary membrane | ISS GO_REF:0000024 | ACCEPT | Summary: Direct mammalian evidence corroborates transferred ciliary-membrane localization. Reason: Independent mammalian domain/localization and trafficking experiments support ciliary-membrane localization. Preserve the original curator-transferred mouse evidence. This is biological corroboration, not a reconstruction of every transfer rule. Supporting Evidence: file:human/ARL13B/ARL13B-uniprot.txt CC -!- SUBCELLULAR LOCATION: Cell projection, cilium membrane CC {ECO:0000269|PubMed:18554500, ECO:0000269|PubMed:24120134}; Lipid- CC anchor {ECO:0000269|PubMed:18554500, ECO:0000269|PubMed:24120134}. |
| GO:0060271 cilium assembly | IEA GO_REF:0000107 | ACCEPT | Summary: Retain the inherited cilium-assembly role. Reason: Retain the existing ortholog-supported cilium-assembly role, corroborated by domain/rescue and ciliary organization studies. ARL13B provides regulatory and tubulin-associated contributions; a direct motor, polymerase or structural-doublet-subunit mechanism is not inferred from loss-of-function phenotypes. Supporting Evidence: PMID:29592971 However, the ectopic expression of GFP-tagged wild-type mARL13B or huARL13B re-established a cilia length of most Arl13b hnn MEFs to be 2.0β4.0β
Β΅m ( Fig.Β 3 E,F) ( Larkins et al., 2011 ). |
| GO:0060271 cilium assembly | ISS GO_REF:0000024 | ACCEPT | Summary: Retain the curator-transferred cilium-assembly role. Reason: Retain the existing ortholog-supported cilium-assembly role, corroborated by domain/rescue and ciliary organization studies. ARL13B provides regulatory and tubulin-associated contributions; a direct motor, polymerase or structural-doublet-subunit mechanism is not inferred from loss-of-function phenotypes. Supporting Evidence: PMID:29592971 However, the ectopic expression of GFP-tagged wild-type mARL13B or huARL13B re-established a cilia length of most Arl13b hnn MEFs to be 2.0β4.0β
Β΅m ( Fig.Β 3 E,F) ( Larkins et al., 2011 ). |
| GO:0097500 receptor localization to non-motile cilium | IBA GO_REF:0000033 | ACCEPT | Summary: Ciliary receptor organization supports the established localization role. Reason: Retain the curated ancestral receptor-localization role. ARL13-family perturbations alter ciliary membrane receptor organization, and the human/mouse G-domain experiments independently link ARL13B to receptor distribution. These observations corroborate the role without claiming that all ciliary receptors share one entry mechanism or that the PAINT tree was re-audited. Supporting Evidence: PMID:29592971 In contrast, in Arl13b hnn MEFs SMO concentrated in discrete regions of the cilium located mostly at distal and/or proximal extremities of cilia regardless the presence of SHH in the medium ( Fig.Β 5 B). However, we noticed that SMO in cilia of SHH-stimulated Arl13b hnn MEFs was almost exclusively detected at the distal tip of the cilium ( Fig.Β 5 B,E). Similarly, the mCherry-SSTR3 also failed to distribute evenly along the cilium and accumulated at the distal tip of Arl13b hnn MEFs cilia ( Fig.Β 5 H). |
| GO:0097730 non-motile cilium | IBA GO_REF:0000033 | ACCEPT | Summary: Non-motile cilia are an independently supported activity site. Reason: Non-motile cilia are a well-established ARL13B activity site, supported by mammalian imaging and the cited exocyst/ciliogenesis study. PAINT including the human target among descendants is legitimate experimental grounding, not circular evidence. Supporting Evidence: file:human/ARL13B/ARL13B-uniprot.txt CC -!- SUBCELLULAR LOCATION: Cell projection, cilium membrane CC {ECO:0000269|PubMed:18554500, ECO:0000269|PubMed:24120134}; Lipid- CC anchor {ECO:0000269|PubMed:18554500, ECO:0000269|PubMed:24120134}. |
| GO:0097731 9+0 non-motile cilium | EXP PMID:26582389 Arl13b and the exocyst interact synergistically in ciliogene... | ACCEPT | Summary: The original exocyst study supports the primary-cilium activity context. Reason: The actual PMID:26582389 study combines mammalian ciliary imaging, conditional mouse renal loss and ciliation analysis with exocyst association. This supports the original 9+0 non-motile-cilium activity context. Human, mouse and canine assays are distinguished; no exocyst-subunit identity is inferred. Supporting Evidence: file:human/ARL13B/ARL13B-uniprot.txt CC -!- SUBCELLULAR LOCATION: Cell projection, cilium membrane CC {ECO:0000269|PubMed:18554500, ECO:0000269|PubMed:24120134}; Lipid- CC anchor {ECO:0000269|PubMed:18554500, ECO:0000269|PubMed:24120134}. |
| GO:0097731 9+0 non-motile cilium | IEA GO_REF:0000120 | ACCEPT | Summary: The combined electronic primary-cilium activity assignment is corroborated. Reason: The combined ARBA/orthology assertion agrees with independent mammalian primary-cilium imaging and functional evidence. The complete ARBA derivation was not reconstructed. Preserve its original is_active_in qualifier. Supporting Evidence: file:human/ARL13B/ARL13B-uniprot.txt CC -!- SUBCELLULAR LOCATION: Cell projection, cilium membrane CC {ECO:0000269|PubMed:18554500, ECO:0000269|PubMed:24120134}; Lipid- CC anchor {ECO:0000269|PubMed:18554500, ECO:0000269|PubMed:24120134}. |
| GO:1905515 non-motile cilium assembly | IBA GO_REF:0000033 | ACCEPT | Summary: Independent mammalian evidence supports the ancestral assembly role. Reason: Retain the established non-motile cilium assembly role, supported by mammalian rescue and organization experiments and the curated orthology/ancestral assertions. This retains existing evidence rather than proposing additional process terms from necessity alone. Supporting Evidence: PMID:29592971 However, the ectopic expression of GFP-tagged wild-type mARL13B or huARL13B re-established a cilia length of most Arl13b hnn MEFs to be 2.0β4.0β
Β΅m ( Fig.Β 3 E,F) ( Larkins et al., 2011 ). |
| GO:1905515 non-motile cilium assembly | IEA GO_REF:0000107 | ACCEPT | Summary: Retain the electronically transferred non-motile-cilium assembly role. Reason: Retain the established non-motile cilium assembly role, supported by mammalian rescue and organization experiments and the curated orthology/ancestral assertions. This retains existing evidence rather than proposing additional process terms from necessity alone. Supporting Evidence: PMID:29592971 However, the ectopic expression of GFP-tagged wild-type mARL13B or huARL13B re-established a cilia length of most Arl13b hnn MEFs to be 2.0β4.0β
Β΅m ( Fig.Β 3 E,F) ( Larkins et al., 2011 ). |
| GO:1905515 non-motile cilium assembly | ISS GO_REF:0000024 | ACCEPT | Summary: Retain the curator-transferred non-motile-cilium assembly role. Reason: Retain the established non-motile cilium assembly role, supported by mammalian rescue and organization experiments and the curated orthology/ancestral assertions. This retains existing evidence rather than proposing additional process terms from necessity alone. Supporting Evidence: PMID:29592971 However, the ectopic expression of GFP-tagged wild-type mARL13B or huARL13B re-established a cilia length of most Arl13b hnn MEFs to be 2.0β4.0β
Β΅m ( Fig.Β 3 E,F) ( Larkins et al., 2011 ). |
| GO:0005085 guanyl-nucleotide exchange factor activity | IDA PMID:26551564 A G-protein activation cascade from Arl13B to Arl3 and impli... | NEW | Summary: Direct biochemical evidence supports this molecular function. Reason: Purified human ARL13B residues 18β278 promote nucleotide exchange on murine ARL3, with human ARL2 as a negative comparator. Mammalian-cell activation and later human ARL13B/BART reconstitution corroborate the activity. Algal structural experiments are distinguished from the human assay, and no equivalence between ARL13B hydrolysis and its exchange-factor function is asserted. Supporting Evidence: PMID:26551564 Finally, we were able to purify human Arl13B (18β278) from insect cells in reasonable amounts to test its GEF activity. Confirming the conservation of structure and function the human Arl13B also exhibits strong GEF activity for Arl3 (Figure 6A). |
| GO:0015631 tubulin binding | IDA PMID:29592971 The Joubert syndrome protein ARL13B binds tubulin to maintai... | NEW | Summary: Direct biochemical evidence supports this molecular function. Reason: The original study directly assays purified human GST-ARL13B binding to bovine tubulin, with GST controls and domain mapping. The mouse TAP bait, mouse cellular rescue and algal axoneme assays are distinct supporting contexts. Retained binding of T35N does not by itself establish independence from GTP hydrolysis, because independent 2017 biochemical measurements show that T35N retains hydrolysis. No motor or polymerase activity is inferred. Supporting Evidence: PMID:29592971 To distinguish between a direct or indirect association of ARL13B with tubulin, we isolated recombinant GST-tagged human ARL13B from E. coli and incubated it in the presence of purified bovine brain tubulin. Incubation of the GST protein tag with tubulins was used as control. Glutathione (GSH) sepharose beads were added to each reaction mix to purify GST-ARL13B or control GST, and their interacting proteins were analyzed by western blotting. Tubulin was found in the eluate from the beads loaded with GST-ARL13B, but not in that from control GST ( Fig.Β 2 A and B, lanes βGST-ARL13B aa 1-428β and βGSTβ), thus indicating that ARL13B can directly interact with tubulin dimers. |
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Download this section (compressed HTML)Q: Which endogenous effectors distinguish ARL13B GTPase cycling from its ARL3 exchange-factor activity?
Q: How do tubulin binding, membrane retention and GEF activity contribute separately to ciliary receptor organization?
Experiment: Validate interface variants biochemically for nucleotide binding, hydrolysis, ARL3 exchange and tubulin binding before endogenous ciliary rescue experiments.
Hypothesis: ARL13B tubulin binding and ARL3 exchange can be separated without assuming T35N is hydrolysis-deficient.
Type: Biochemical separation of function and endogenous complementation
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