ARMCX1

Summary: Protein binding (IPI) with COIL, from the HI-II-14 binary yeast two-hybrid map. Generic protein binding carries no functional information and no specific term captures this interaction, so the row is removed. Removal does not mean the interaction is false.

Reason: Removed under the generic protein-binding policy: GO:0005515 is uninformative on its own.

Summary: Protein binding (IPI) with ZBTB8A, from the HI-II-14 binary yeast two-hybrid map. Generic protein binding carries no functional information and no specific term captures this interaction, so the row is removed. Removal does not mean the interaction is false.

Reason: Removed under the generic protein-binding policy: GO:0005515 is uninformative on its own.

Summary: Protein binding (IPI) with TFIP11, from the HI-II-14 binary yeast two-hybrid map. Generic protein binding carries no functional information and no specific term captures this interaction, so the row is removed. Removal does not mean the interaction is false.

Reason: Removed under the generic protein-binding policy: GO:0005515 is uninformative on its own.

Summary: Protein binding (IPI) with TFIP11, from a yeast two-hybrid study of variant effects. Generic protein binding carries no functional information and no specific term captures this interaction, so the row is removed. Removal does not mean the interaction is false.

Reason: Removed under the generic protein-binding policy: GO:0005515 is uninformative on its own.

Summary: Protein binding (IPI) with CEP70, from the HuRI binary yeast two-hybrid map. Generic protein binding carries no functional information and no specific term captures this interaction, so the row is removed. Removal does not mean the interaction is false.

Reason: Removed under the generic protein-binding policy: GO:0005515 is uninformative on its own.

Summary: Protein binding (IPI) with GPKOW, from the HuRI binary yeast two-hybrid map. Generic protein binding carries no functional information and no specific term captures this interaction, so the row is removed. Removal does not mean the interaction is false.

Reason: Removed under the generic protein-binding policy: GO:0005515 is uninformative on its own.

Summary: Protein binding (IPI) with ZBTB8A, from the HuRI binary yeast two-hybrid map. Generic protein binding carries no functional information and no specific term captures this interaction, so the row is removed. Removal does not mean the interaction is false.

Reason: Removed under the generic protein-binding policy: GO:0005515 is uninformative on its own.

Summary: Protein binding (IPI) with PICK1, from the HuRI binary yeast two-hybrid map. Generic protein binding carries no functional information and no specific term captures this interaction, so the row is removed. Removal does not mean the interaction is false.

Reason: Removed under the generic protein-binding policy: GO:0005515 is uninformative on its own.

Summary: Mitochondrion (IBA). Mouse Armcx1 is mitochondria-localized, and its neuroprotective and regenerative effects depend on that localization. This row infers the location from the ARMC10/ARMCX family node. Core location.

Reason: [PMID:28009275 "In an established mouse model with robust axon regeneration, we show that Armcx1, a mammalian-specific gene encoding a mitochondria-localized protein, is upregulated after axotomy in this high regeneration condition."] [PMID:28009275 "We further showed that the effects of Armcx1 on neuronal survival and axon regeneration were dependent on its mitochondrial localization."]

Summary: Mitochondrion (IDA). Mouse Armcx1 is mitochondria-localized, and its neuroprotective and regenerative effects depend on that localization. This row is the Human Protein Atlas antibody staining of human ARMCX1 (GO_REF:0000052), the only direct human localization evidence. Core location.

Reason: This row is the Human Protein Atlas antibody staining of human ARMCX1 (GO_REF:0000052), the only direct human localization evidence. [PMID:28009275 "In an established mouse model with robust axon regeneration, we show that Armcx1, a mammalian-specific gene encoding a mitochondria-localized protein, is upregulated after axotomy in this high regeneration condition."]

Summary: Mitochondrion (IEA). Mouse Armcx1 is mitochondria-localized, and its neuroprotective and regenerative effects depend on that localization. This row derives from UniProt's ARBA and SubCell mappings. Core location.

Reason: [PMID:28009275 "In an established mouse model with robust axon regeneration, we show that Armcx1, a mammalian-specific gene encoding a mitochondria-localized protein, is upregulated after axotomy in this high regeneration condition."] [PMID:28009275 "We further showed that the effects of Armcx1 on neuronal survival and axon regeneration were dependent on its mitochondrial localization."]

Summary: Mitochondrion (ISS). Mouse Armcx1 is mitochondria-localized, and its neuroprotective and regenerative effects depend on that localization. This row transfers the mouse Armcx1 localization. Core location.

Reason: [PMID:28009275 "In an established mouse model with robust axon regeneration, we show that Armcx1, a mammalian-specific gene encoding a mitochondria-localized protein, is upregulated after axotomy in this high regeneration condition."] [PMID:28009275 "We further showed that the effects of Armcx1 on neuronal survival and axon regeneration were dependent on its mitochondrial localization."]

Summary: Mitochondrial outer membrane (IEA). Armcx1 has a putative outer-membrane targeting sequence flanking its transmembrane segment, binds the outer-membrane adaptor Miro1, and is described as an outer-membrane protein of neurons. Accepted.

Reason: [PMID:28009275 "Similar to other members of the Armcx cluster, Armcx1 has a putative mitochondrial outer membrane-targeting sequence flanking the trans-membrane (TM) domain (Figure 1D)"] [PMID:28009275 "Co-immunoprecipitation using Armcx1-HA and Miro1-Myc showed that Armcx1 interacted with Miro1 (Figure 1H), the protein linking mitochondria to the Trak1-Kinesin motor complex."] [PMID:37454781 "Armcx1 is highly expressed in the brain and is located in the mitochondrial outer membrane of neurons, where it mediates mitochondrial transport."]

Summary: Mitochondrial outer membrane (ISS). Armcx1 has a putative outer-membrane targeting sequence flanking its transmembrane segment, binds the outer-membrane adaptor Miro1, and is described as an outer-membrane protein of neurons. Accepted.

Reason: [PMID:28009275 "Similar to other members of the Armcx cluster, Armcx1 has a putative mitochondrial outer membrane-targeting sequence flanking the trans-membrane (TM) domain (Figure 1D)"] [PMID:28009275 "Co-immunoprecipitation using Armcx1-HA and Miro1-Myc showed that Armcx1 interacted with Miro1 (Figure 1H), the protein linking mitochondria to the Trak1-Kinesin motor complex."] [PMID:37454781 "Armcx1 is highly expressed in the brain and is located in the mitochondrial outer membrane of neurons, where it mediates mitochondrial transport."]

Summary: Axonal transport of mitochondrion (IBA). The node's seed is mouse Armcx3, which regulates neuronal mitochondrial trafficking through the Kinesin/Miro/Trak2 complex. Mouse Armcx1 itself binds Miro1, the adaptor that couples mitochondria to the Trak-kinesin motor, and enhances mitochondrial transport in retinal ganglion cell axons. Armcx1 knockdown after brain injury impairs mitochondrial transport and lowers Miro1. Participation rather than regulation is chosen because Armcx1 sits on the transported organelle and engages the transport adaptor, as Miro and TRAK do, although the papers describe its effect as controlling the motile pool. Accepted.

Reason: [PMID:22569362 "Furthermore, we demonstrate that Armcx3 expression levels regulate mitochondrial dynamics and trafficking in neurons, and that Alex3 interacts with the Kinesin/Miro/Trak2 complex in a Ca(2+)-dependent manner."] [PMID:28009275 "Co-immunoprecipitation using Armcx1-HA and Miro1-Myc showed that Armcx1 interacted with Miro1 (Figure 1H), the protein linking mitochondria to the Trak1-Kinesin motor complex."] [PMID:28009275 "Armcx1 overexpression enhances mitochondrial transport in adult retinal ganglion cells (RGCs)."] [PMID:38492796 "In addition, inhibition of Armcx1 expression further led to impaired mitochondrial transport, abnormal morphology, reduced ATP levels, aggravation of neuronal apoptosis and neurological dysfunction, and decrease Miro1 expression."]

Summary: Axon cytoplasm (IEA, a GO inter-ontology inference from the axonal transport process). Axonal mitochondria, with ARMCX1 on their outer membrane, lie within the axon cytoplasm, so the term is not wrong. It is derivative of the process row and less precise than the outer-membrane location; kept as non-core.

Reason: [PMID:28009275 "Similar to other members of the Armcx cluster, Armcx1 has a putative mitochondrial outer membrane-targeting sequence flanking the trans-membrane (TM) domain (Figure 1D)"] [PMID:28009275 "Armcx1 overexpression enhances mitochondrial transport in adult retinal ganglion cells (RGCs)."]