ATP5MJ encodes subunit j (6.8-kDa mitochondrial proteolipid, MLQ) of mitochondrial F1F0 ATP synthase (complex V). It is a small single-pass inner-membrane protein in the F(o) membrane domain. Although it is a minor, loosely associated subunit, it is needed to maintain the cellular population of ATP synthase. Its knockdown depletes the enzyme post-transcriptionally, lowers mitochondrial ATP synthesis, slows growth and sensitizes cells to glucose deprivation.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005739 mitochondrion | HTP PMID:34800366 Quantitative high-confidence human mitochondrial proteome an... | ACCEPT | Summary: Mitochondrion (HTP). It is in the high-confidence mitochondrial proteome. Subunit j is a single-pass inner-membrane F(o) subunit of complex V. Accepted. Reason: [file:human/ATP5MJ/ATP5MJ-uniprot.txt "CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex"] [file:human/ATP5MJ/ATP5MJ-uniprot.txt "FT TRANSMEM 22..39"] [PMID:24330338 "A 6.8-kDa proteolipid (called MLQ) is a hydrophobic mitochondrial protein with unknown function that is loosely associated with ATP synthase."] Supporting Evidence: file:human/ATP5MJ/ATP5MJ-uniprot.txt CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex file:human/ATP5MJ/ATP5MJ-uniprot.txt FT TRANSMEM 22..39 PMID:24330338 A 6.8-kDa proteolipid (called MLQ) is a hydrophobic mitochondrial protein with unknown function that is loosely associated with ATP synthase. |
| GO:0005739 mitochondrion | IDA GO_REF:0000052 | ACCEPT | Summary: Mitochondrion (IDA). Human Protein Atlas immunofluorescence. Subunit j is a single-pass inner-membrane F(o) subunit of complex V. Accepted. Reason: [file:human/ATP5MJ/ATP5MJ-uniprot.txt "CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex"] [file:human/ATP5MJ/ATP5MJ-uniprot.txt "FT TRANSMEM 22..39"] [PMID:24330338 "A 6.8-kDa proteolipid (called MLQ) is a hydrophobic mitochondrial protein with unknown function that is loosely associated with ATP synthase."] Supporting Evidence: file:human/ATP5MJ/ATP5MJ-uniprot.txt CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex file:human/ATP5MJ/ATP5MJ-uniprot.txt FT TRANSMEM 22..39 PMID:24330338 A 6.8-kDa proteolipid (called MLQ) is a hydrophobic mitochondrial protein with unknown function that is loosely associated with ATP synthase. |
| GO:0005739 mitochondrion | IEA GO_REF:0000002 | ACCEPT | Summary: Mitochondrion (IEA). Subunit j is a single-pass inner-membrane F(o) subunit of complex V. Accepted. Reason: [file:human/ATP5MJ/ATP5MJ-uniprot.txt "CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex"] [file:human/ATP5MJ/ATP5MJ-uniprot.txt "FT TRANSMEM 22..39"] [PMID:24330338 "A 6.8-kDa proteolipid (called MLQ) is a hydrophobic mitochondrial protein with unknown function that is loosely associated with ATP synthase."] Supporting Evidence: file:human/ATP5MJ/ATP5MJ-uniprot.txt CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex file:human/ATP5MJ/ATP5MJ-uniprot.txt FT TRANSMEM 22..39 PMID:24330338 A 6.8-kDa proteolipid (called MLQ) is a hydrophobic mitochondrial protein with unknown function that is loosely associated with ATP synthase. |
| GO:0005743 mitochondrial inner membrane | NAS PMID:26297831 Assembly of human mitochondrial ATP synthase through two sep... | ACCEPT | Summary: Mitochondrial inner membrane (NAS). Subunit j is a single-pass inner-membrane F(o) subunit of complex V. Accepted. Reason: [file:human/ATP5MJ/ATP5MJ-uniprot.txt "CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex"] [file:human/ATP5MJ/ATP5MJ-uniprot.txt "FT TRANSMEM 22..39"] [PMID:24330338 "A 6.8-kDa proteolipid (called MLQ) is a hydrophobic mitochondrial protein with unknown function that is loosely associated with ATP synthase."] Supporting Evidence: file:human/ATP5MJ/ATP5MJ-uniprot.txt CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex file:human/ATP5MJ/ATP5MJ-uniprot.txt FT TRANSMEM 22..39 PMID:24330338 A 6.8-kDa proteolipid (called MLQ) is a hydrophobic mitochondrial protein with unknown function that is loosely associated with ATP synthase. |
| GO:0005743 mitochondrial inner membrane | TAS Reactome:R-HSA-164832 | ACCEPT | Summary: Mitochondrial inner membrane (TAS). Subunit j is a single-pass inner-membrane F(o) subunit of complex V. The Reactome event is 'ATPase synthesizes ATP'. Accepted. Reason: [file:human/ATP5MJ/ATP5MJ-uniprot.txt "CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex"] [file:human/ATP5MJ/ATP5MJ-uniprot.txt "FT TRANSMEM 22..39"] [PMID:24330338 "A 6.8-kDa proteolipid (called MLQ) is a hydrophobic mitochondrial protein with unknown function that is loosely associated with ATP synthase."] Supporting Evidence: file:human/ATP5MJ/ATP5MJ-uniprot.txt CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex file:human/ATP5MJ/ATP5MJ-uniprot.txt FT TRANSMEM 22..39 PMID:24330338 A 6.8-kDa proteolipid (called MLQ) is a hydrophobic mitochondrial protein with unknown function that is loosely associated with ATP synthase. |
| GO:0005743 mitochondrial inner membrane | TAS Reactome:R-HSA-164834 | ACCEPT | Summary: Mitochondrial inner membrane (TAS). Subunit j is a single-pass inner-membrane F(o) subunit of complex V. The Reactome event is 'Enzyme-bound ATP is released'. Accepted. Reason: [file:human/ATP5MJ/ATP5MJ-uniprot.txt "CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex"] [file:human/ATP5MJ/ATP5MJ-uniprot.txt "FT TRANSMEM 22..39"] [PMID:24330338 "A 6.8-kDa proteolipid (called MLQ) is a hydrophobic mitochondrial protein with unknown function that is loosely associated with ATP synthase."] Supporting Evidence: file:human/ATP5MJ/ATP5MJ-uniprot.txt CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex file:human/ATP5MJ/ATP5MJ-uniprot.txt FT TRANSMEM 22..39 PMID:24330338 A 6.8-kDa proteolipid (called MLQ) is a hydrophobic mitochondrial protein with unknown function that is loosely associated with ATP synthase. |
| GO:0005743 mitochondrial inner membrane | TAS Reactome:R-HSA-164840 | ACCEPT | Summary: Mitochondrial inner membrane (TAS). Subunit j is a single-pass inner-membrane F(o) subunit of complex V. The Reactome event is 'ADP and Pi bind to ATPase'. Accepted. Reason: [file:human/ATP5MJ/ATP5MJ-uniprot.txt "CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex"] [file:human/ATP5MJ/ATP5MJ-uniprot.txt "FT TRANSMEM 22..39"] [PMID:24330338 "A 6.8-kDa proteolipid (called MLQ) is a hydrophobic mitochondrial protein with unknown function that is loosely associated with ATP synthase."] Supporting Evidence: file:human/ATP5MJ/ATP5MJ-uniprot.txt CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex file:human/ATP5MJ/ATP5MJ-uniprot.txt FT TRANSMEM 22..39 PMID:24330338 A 6.8-kDa proteolipid (called MLQ) is a hydrophobic mitochondrial protein with unknown function that is loosely associated with ATP synthase. |
| GO:0005743 mitochondrial inner membrane | TAS Reactome:R-HSA-8949580 | ACCEPT | Summary: Mitochondrial inner membrane (TAS). Subunit j is a single-pass inner-membrane F(o) subunit of complex V. The Reactome event is 'F1Fo ATP synthase dimerizes'. Accepted. Reason: [file:human/ATP5MJ/ATP5MJ-uniprot.txt "CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex"] [file:human/ATP5MJ/ATP5MJ-uniprot.txt "FT TRANSMEM 22..39"] [PMID:24330338 "A 6.8-kDa proteolipid (called MLQ) is a hydrophobic mitochondrial protein with unknown function that is loosely associated with ATP synthase."] Supporting Evidence: file:human/ATP5MJ/ATP5MJ-uniprot.txt CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex file:human/ATP5MJ/ATP5MJ-uniprot.txt FT TRANSMEM 22..39 PMID:24330338 A 6.8-kDa proteolipid (called MLQ) is a hydrophobic mitochondrial protein with unknown function that is loosely associated with ATP synthase. |
| GO:0015986 proton motive force-driven ATP synthesis | NAS PMID:26297831 Assembly of human mitochondrial ATP synthase through two sep... | ACCEPT | Summary: Proton motive force-driven ATP synthesis (NAS, ComplexPortal). Subunit j knockdown lowers mitochondrial ATP synthesis by reducing the amount of ATP synthase. As a complex V subunit it takes part in the process. Core. Reason: [file:human/ATP5MJ/ATP5MJ-uniprot.txt "CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex"] [PMID:24330338 "As a consequence, the knockdown cells show low mitochondrial ATP synthesis activity, grow slowly in the normal medium, and are vulnerable to glucose deprivation."] Supporting Evidence: file:human/ATP5MJ/ATP5MJ-uniprot.txt CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex PMID:24330338 As a consequence, the knockdown cells show low mitochondrial ATP synthesis activity, grow slowly in the normal medium, and are vulnerable to glucose deprivation. |
| GO:0031966 mitochondrial membrane | EXP PMID:24330338 Population of ATP synthase molecules in mitochondria is limi... | ACCEPT | Summary: Mitochondrial membrane (EXP). MLQ is a hydrophobic mitochondrial proteolipid associated with ATP synthase. Subunit j is a single-pass inner-membrane F(o) subunit of complex V. Accepted. Reason: [file:human/ATP5MJ/ATP5MJ-uniprot.txt "CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex"] [file:human/ATP5MJ/ATP5MJ-uniprot.txt "FT TRANSMEM 22..39"] [PMID:24330338 "A 6.8-kDa proteolipid (called MLQ) is a hydrophobic mitochondrial protein with unknown function that is loosely associated with ATP synthase."] Supporting Evidence: file:human/ATP5MJ/ATP5MJ-uniprot.txt CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex file:human/ATP5MJ/ATP5MJ-uniprot.txt FT TRANSMEM 22..39 PMID:24330338 A 6.8-kDa proteolipid (called MLQ) is a hydrophobic mitochondrial protein with unknown function that is loosely associated with ATP synthase. |
| GO:0031966 mitochondrial membrane | IEA GO_REF:0000044 | ACCEPT | Summary: Mitochondrial membrane (IEA). Subunit j is a single-pass inner-membrane F(o) subunit of complex V. Accepted. Reason: [file:human/ATP5MJ/ATP5MJ-uniprot.txt "CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex"] [file:human/ATP5MJ/ATP5MJ-uniprot.txt "FT TRANSMEM 22..39"] [PMID:24330338 "A 6.8-kDa proteolipid (called MLQ) is a hydrophobic mitochondrial protein with unknown function that is loosely associated with ATP synthase."] Supporting Evidence: file:human/ATP5MJ/ATP5MJ-uniprot.txt CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex file:human/ATP5MJ/ATP5MJ-uniprot.txt FT TRANSMEM 22..39 PMID:24330338 A 6.8-kDa proteolipid (called MLQ) is a hydrophobic mitochondrial protein with unknown function that is loosely associated with ATP synthase. |
| GO:0045259 proton-transporting ATP synthase complex | IBA GO_REF:0000033 | ACCEPT | Summary: Proton-transporting ATP synthase complex (IBA). Subunit j (6.8-kDa proteolipid, MLQ) is a single-pass F(o) subunit of complex V in the human structure, and its knockdown depletes mitochondrial ATP synthase. Core. Reason: [file:human/ATP5MJ/ATP5MJ-uniprot.txt "CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex"] [file:human/ATP5MJ/ATP5MJ-uniprot.txt "CC F(0) domain (PubMed:37244256). Minor subunit required to maintain the"] [PMID:24330338 "Here, we show that MLQ-knockdown HeLa cells lose population of ATP synthase in mitochondria."] Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000391214 SUPPORTS TRANSFER Subunit j family node seeded by bovine (P14790) and rat data; human subunit j is resolved in the human ATP synthase structure. Supporting Evidence: file:human/ATP5MJ/ATP5MJ-uniprot.txt CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex file:human/ATP5MJ/ATP5MJ-uniprot.txt CC F(0) domain (PubMed:37244256). Minor subunit required to maintain the PMID:24330338 Here, we show that MLQ-knockdown HeLa cells lose population of ATP synthase in mitochondria. |
| GO:0045259 proton-transporting ATP synthase complex | ISS GO_REF:0000024 | ACCEPT | Summary: Proton-transporting ATP synthase complex (ISS, from bovine subunit j (P14790)). Subunit j (6.8-kDa proteolipid, MLQ) is a single-pass F(o) subunit of complex V in the human structure, and its knockdown depletes mitochondrial ATP synthase. Core. Reason: [file:human/ATP5MJ/ATP5MJ-uniprot.txt "CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex"] [file:human/ATP5MJ/ATP5MJ-uniprot.txt "CC F(0) domain (PubMed:37244256). Minor subunit required to maintain the"] [PMID:24330338 "Here, we show that MLQ-knockdown HeLa cells lose population of ATP synthase in mitochondria."] Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:P14790 SUPPORTS TRANSFER Bovine subunit j (ortholog), a component of purified bovine complex V. Supporting Evidence: file:human/ATP5MJ/ATP5MJ-uniprot.txt CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex file:human/ATP5MJ/ATP5MJ-uniprot.txt CC F(0) domain (PubMed:37244256). Minor subunit required to maintain the PMID:24330338 Here, we show that MLQ-knockdown HeLa cells lose population of ATP synthase in mitochondria. |
| GO:0045259 proton-transporting ATP synthase complex | NAS PMID:26297831 Assembly of human mitochondrial ATP synthase through two sep... | ACCEPT | Summary: Proton-transporting ATP synthase complex (NAS, ComplexPortal). Subunit j (6.8-kDa proteolipid, MLQ) is a single-pass F(o) subunit of complex V in the human structure, and its knockdown depletes mitochondrial ATP synthase. Core. Reason: [file:human/ATP5MJ/ATP5MJ-uniprot.txt "CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex"] [file:human/ATP5MJ/ATP5MJ-uniprot.txt "CC F(0) domain (PubMed:37244256). Minor subunit required to maintain the"] [PMID:24330338 "Here, we show that MLQ-knockdown HeLa cells lose population of ATP synthase in mitochondria."] Supporting Evidence: file:human/ATP5MJ/ATP5MJ-uniprot.txt CC -!- FUNCTION: Subunit j, of the mitochondrial membrane ATP synthase complex file:human/ATP5MJ/ATP5MJ-uniprot.txt CC F(0) domain (PubMed:37244256). Minor subunit required to maintain the PMID:24330338 Here, we show that MLQ-knockdown HeLa cells lose population of ATP synthase in mitochondria. |
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Download this section (compressed HTML)Q: Does loss of subunit j block a specific ATP synthase assembly intermediate, or does it increase turnover of the assembled complex?
Experiment: Knock out ATP5MJ and profile complex V assembly intermediates by complexome profiling, and measure the half-life of assembled ATP synthase with pulse-chase labelling.
What is not known β curated, literature-grounded statements of the open unknowns (the inverse of core functions).
Gap: How subunit j maintains ATP synthase abundance, whether by promoting assembly, stabilizing the assembled complex or protecting it from turnover, is unknown.
OPEN BIOLOGY
What is known: Established: subunit j is an F(o) subunit, and its knockdown depletes ATP synthase without lowering alpha/beta mRNA. Not established: the step at which subunit j acts, or how its variable expression is regulated.
Provenance (the field's own admissions):
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