AURKAIP1 (Aurora kinase A-interacting protein 1) is the mitochondrial small ribosomal subunit protein bS22m (formerly mS38/MRPS38). It is a component of the small subunit of the mitoribosome in the mitochondrial matrix and is required for synthesis of mitochondrially encoded proteins. The family is conserved in some bacterial ribosomes. The yeast ortholog Cox24/mS38 is needed for efficient mitoribosome assembly and preferentially for translation initiation of COX1-3 mRNAs. AURKAIP1 was first identified as an Aurora-A interactor whose ectopic expression promotes proteasome-dependent, ubiquitin-independent, antizyme1-mediated Aurora-A degradation, and a nuclear pool has been reported.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005515 protein binding | IPI PMID:12244051 Aurora-A kinase interacting protein (AIP), a novel negative ... | MODIFY | Summary: Protein binding (IPI) with Aurora-A, from the 2002 study that named AIP as an Aurora-A interactor whose overexpression destabilizes Aurora-A. Protein kinase binding is the informative term. It is kept separate from AURKAIP1's established role as a mitoribosomal protein. Reason: [PMID:12244051 "Ectopic expression of AIP with Aurora-A in NIH 3T3 and COS cells results in the down-regulation of ectopically expressed Aurora-A protein levels, and this down-regulation is demonstrated to be the result of destabilization of Aurora-A through a proteasome-dependent protein degradation pathway."] Proposed replacements: protein kinase binding Supporting Evidence: PMID:12244051 Ectopic expression of AIP with Aurora-A in NIH 3T3 and COS cells results in the down-regulation of ectopically expressed Aurora-A protein levels, and this down-regulation is demonstrated to be the result of destabilization of Aurora-A through a proteasome-dependent protein degradation pathway. |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: Protein binding (IPI) with FAM9B, from a large-scale Y2H map. No functional link to the mitoribosome. Generic protein binding is uninformative; removed under policy. Removal does not mean the interaction is false. Reason: Removed under the generic protein-binding policy: GO:0005515 is uninformative on its own. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Protein binding (IPI) with ABHD4, from a large-scale Y2H map. No functional link to the mitoribosome. Generic protein binding is uninformative; removed under policy. Removal does not mean the interaction is false. Reason: Removed under the generic protein-binding policy: GO:0005515 is uninformative on its own. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Protein binding (IPI) with DEFB127, from a large-scale Y2H map. No functional link to the mitoribosome. Generic protein binding is uninformative; removed under policy. Removal does not mean the interaction is false. Reason: Removed under the generic protein-binding policy: GO:0005515 is uninformative on its own. |
| GO:0005634 nucleus | IDA PMID:12244051 Aurora-A kinase interacting protein (AIP), a novel negative ... | KEEP AS NON CORE | Summary: Nucleus (IDA). UniProt records a nuclear location (attributed to PMID:23908630), in addition to the matrix. That paper notes that ectopically expressed AURKAIP1 has been localized to the nucleus, and the 2002 AIP study described it as nuclear. HPA reports nucleoplasm. Its established function is in the mitochondrial matrix, and the nuclear pool's role is unclear. Kept as non-core. Reason: [file:human/AURKAIP1/AURKAIP1-uniprot.txt "CC {ECO:0000269|PubMed:23908630, ECO:0000269|PubMed:27184847}. Nucleus"] [PMID:23908630 "Ectopically expressed forms of CRIF1 and AURKAIP1 have been localized to the nucleus and cytosol and reported to be involved in the transcriptional activation and regulation of Aurora-A kinase in human cell lines, respectively (Kang et al., 2010)."] [PMID:12244051 "AIP is a ubiquitously expressed nuclear protein that interacts specifically with human Aurora-A in vivo."] Supporting Evidence: file:human/AURKAIP1/AURKAIP1-uniprot.txt CC {ECO:0000269|PubMed:23908630, ECO:0000269|PubMed:27184847}. Nucleus PMID:23908630 Ectopically expressed forms of CRIF1 and AURKAIP1 have been localized to the nucleus and cytosol and reported to be involved in the transcriptional activation and regulation of Aurora-A kinase in human cell lines, respectively (Kang et al., 2010). PMID:12244051 AIP is a ubiquitously expressed nuclear protein that interacts specifically with human Aurora-A in vivo. file:human/AURKAIP1/AURKAIP1-uniprot.txt CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix |
| GO:0005634 nucleus | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: Nucleus (IEA). UniProt records a nuclear location (attributed to PMID:23908630), in addition to the matrix. That paper notes that ectopically expressed AURKAIP1 has been localized to the nucleus, and the 2002 AIP study described it as nuclear. HPA reports nucleoplasm. Its established function is in the mitochondrial matrix, and the nuclear pool's role is unclear. Kept as non-core. Reason: [file:human/AURKAIP1/AURKAIP1-uniprot.txt "CC {ECO:0000269|PubMed:23908630, ECO:0000269|PubMed:27184847}. Nucleus"] [PMID:23908630 "Ectopically expressed forms of CRIF1 and AURKAIP1 have been localized to the nucleus and cytosol and reported to be involved in the transcriptional activation and regulation of Aurora-A kinase in human cell lines, respectively (Kang et al., 2010)."] [PMID:12244051 "AIP is a ubiquitously expressed nuclear protein that interacts specifically with human Aurora-A in vivo."] Supporting Evidence: file:human/AURKAIP1/AURKAIP1-uniprot.txt CC {ECO:0000269|PubMed:23908630, ECO:0000269|PubMed:27184847}. Nucleus PMID:23908630 Ectopically expressed forms of CRIF1 and AURKAIP1 have been localized to the nucleus and cytosol and reported to be involved in the transcriptional activation and regulation of Aurora-A kinase in human cell lines, respectively (Kang et al., 2010). PMID:12244051 AIP is a ubiquitously expressed nuclear protein that interacts specifically with human Aurora-A in vivo. file:human/AURKAIP1/AURKAIP1-uniprot.txt CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix |
| GO:0005654 nucleoplasm | IDA GO_REF:0000052 | KEEP AS NON CORE | Summary: Nucleoplasm (IDA). UniProt records a nuclear location (attributed to PMID:23908630), in addition to the matrix. That paper notes that ectopically expressed AURKAIP1 has been localized to the nucleus, and the 2002 AIP study described it as nuclear. HPA reports nucleoplasm. Its established function is in the mitochondrial matrix, and the nuclear pool's role is unclear. Kept as non-core. Reason: [file:human/AURKAIP1/AURKAIP1-uniprot.txt "CC {ECO:0000269|PubMed:23908630, ECO:0000269|PubMed:27184847}. Nucleus"] [PMID:23908630 "Ectopically expressed forms of CRIF1 and AURKAIP1 have been localized to the nucleus and cytosol and reported to be involved in the transcriptional activation and regulation of Aurora-A kinase in human cell lines, respectively (Kang et al., 2010)."] [PMID:12244051 "AIP is a ubiquitously expressed nuclear protein that interacts specifically with human Aurora-A in vivo."] Supporting Evidence: file:human/AURKAIP1/AURKAIP1-uniprot.txt CC {ECO:0000269|PubMed:23908630, ECO:0000269|PubMed:27184847}. Nucleus PMID:23908630 Ectopically expressed forms of CRIF1 and AURKAIP1 have been localized to the nucleus and cytosol and reported to be involved in the transcriptional activation and regulation of Aurora-A kinase in human cell lines, respectively (Kang et al., 2010). PMID:12244051 AIP is a ubiquitously expressed nuclear protein that interacts specifically with human Aurora-A in vivo. file:human/AURKAIP1/AURKAIP1-uniprot.txt CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix |
| GO:0005739 mitochondrion | HTP PMID:34800366 Quantitative high-confidence human mitochondrial proteome an... | ACCEPT | Summary: Mitochondrion (HTP). As a mitoribosomal protein, AURKAIP1 sits in the matrix-facing small subunit of the 55S mitoribosome. Mammalian mitoribosomes are tethered to the inner membrane for co-translational insertion of their hydrophobic products, so a membrane-associated assignment for the whole particle is defensible. Accepted. Reason: [file:human/AURKAIP1/AURKAIP1-uniprot.txt "CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix"] [PMID:23908630 "The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in"] Supporting Evidence: file:human/AURKAIP1/AURKAIP1-uniprot.txt CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix PMID:23908630 The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in |
| GO:0005739 mitochondrion | IBA GO_REF:0000033 | ACCEPT | Summary: Mitochondrion (IBA). As a mitoribosomal protein, AURKAIP1 sits in the matrix-facing small subunit of the 55S mitoribosome. Mammalian mitoribosomes are tethered to the inner membrane for co-translational insertion of their hydrophobic products, so a membrane-associated assignment for the whole particle is defensible. Accepted. Reason: [file:human/AURKAIP1/AURKAIP1-uniprot.txt "CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix"] [PMID:23908630 "The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in"] Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN001593291 SUPPORTS TRANSFER mS38/Cox24 family node with fungal and human members; mitochondrial location is conserved. Supporting Evidence: file:human/AURKAIP1/AURKAIP1-uniprot.txt CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix PMID:23908630 The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in |
| GO:0005739 mitochondrion | IDA GO_REF:0000052 | ACCEPT | Summary: Mitochondrion (IDA). As a mitoribosomal protein, AURKAIP1 sits in the matrix-facing small subunit of the 55S mitoribosome. Mammalian mitoribosomes are tethered to the inner membrane for co-translational insertion of their hydrophobic products, so a membrane-associated assignment for the whole particle is defensible. Accepted. Reason: [file:human/AURKAIP1/AURKAIP1-uniprot.txt "CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix"] [PMID:23908630 "The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in"] Supporting Evidence: file:human/AURKAIP1/AURKAIP1-uniprot.txt CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix PMID:23908630 The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in |
| GO:0005743 mitochondrial inner membrane | NAS PMID:27023846 Structure and Function of the Mitochondrial Ribosome. | ACCEPT | Summary: Mitochondrial inner membrane (NAS). As a mitoribosomal protein, AURKAIP1 sits in the matrix-facing small subunit of the 55S mitoribosome. Mammalian mitoribosomes are tethered to the inner membrane for co-translational insertion of their hydrophobic products, so a membrane-associated assignment for the whole particle is defensible. Accepted. Reason: [file:human/AURKAIP1/AURKAIP1-uniprot.txt "CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix"] [PMID:23908630 "The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in"] Supporting Evidence: file:human/AURKAIP1/AURKAIP1-uniprot.txt CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix PMID:23908630 The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in |
| GO:0005743 mitochondrial inner membrane | TAS Reactome:R-HSA-5419261 | ACCEPT | Summary: Mitochondrial inner membrane (TAS). As a mitoribosomal protein, AURKAIP1 sits in the matrix-facing small subunit of the 55S mitoribosome. Mammalian mitoribosomes are tethered to the inner membrane for co-translational insertion of their hydrophobic products, so a membrane-associated assignment for the whole particle is defensible. Reactome event: '55S ribosome with peptidyl-tRNA in A site binds GFM1:GTP'. Accepted. Reason: [file:human/AURKAIP1/AURKAIP1-uniprot.txt "CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix"] [PMID:23908630 "The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in"] Supporting Evidence: file:human/AURKAIP1/AURKAIP1-uniprot.txt CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix PMID:23908630 The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in |
| GO:0005743 mitochondrial inner membrane | TAS Reactome:R-HSA-5419264 | ACCEPT | Summary: Mitochondrial inner membrane (TAS). As a mitoribosomal protein, AURKAIP1 sits in the matrix-facing small subunit of the 55S mitoribosome. Mammalian mitoribosomes are tethered to the inner membrane for co-translational insertion of their hydrophobic products, so a membrane-associated assignment for the whole particle is defensible. Reactome event: 'MTRF1L (MTRF1a) or ICT1 binds stop codon in 55S ribosome:mRNA:peptidyl-tRNA'. Accepted. Reason: [file:human/AURKAIP1/AURKAIP1-uniprot.txt "CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix"] [PMID:23908630 "The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in"] Supporting Evidence: file:human/AURKAIP1/AURKAIP1-uniprot.txt CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix PMID:23908630 The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in |
| GO:0005743 mitochondrial inner membrane | TAS Reactome:R-HSA-5419271 | ACCEPT | Summary: Mitochondrial inner membrane (TAS). As a mitoribosomal protein, AURKAIP1 sits in the matrix-facing small subunit of the 55S mitoribosome. Mammalian mitoribosomes are tethered to the inner membrane for co-translational insertion of their hydrophobic products, so a membrane-associated assignment for the whole particle is defensible. Reactome event: '55S ribosome:MTRF1L hydrolyzes peptidyl-tRNA bond and GTP is hydrolyzed'. Accepted. Reason: [file:human/AURKAIP1/AURKAIP1-uniprot.txt "CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix"] [PMID:23908630 "The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in"] Supporting Evidence: file:human/AURKAIP1/AURKAIP1-uniprot.txt CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix PMID:23908630 The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in |
| GO:0005743 mitochondrial inner membrane | TAS Reactome:R-HSA-5419273 | ACCEPT | Summary: Mitochondrial inner membrane (TAS). As a mitoribosomal protein, AURKAIP1 sits in the matrix-facing small subunit of the 55S mitoribosome. Mammalian mitoribosomes are tethered to the inner membrane for co-translational insertion of their hydrophobic products, so a membrane-associated assignment for the whole particle is defensible. Reactome event: 'Hydrolysis of GTP and dissociation of 28S and 39S subunits'. Accepted. Reason: [file:human/AURKAIP1/AURKAIP1-uniprot.txt "CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix"] [PMID:23908630 "The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in"] Supporting Evidence: file:human/AURKAIP1/AURKAIP1-uniprot.txt CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix PMID:23908630 The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in |
| GO:0005743 mitochondrial inner membrane | TAS Reactome:R-HSA-5419277 | ACCEPT | Summary: Mitochondrial inner membrane (TAS). As a mitoribosomal protein, AURKAIP1 sits in the matrix-facing small subunit of the 55S mitoribosome. Mammalian mitoribosomes are tethered to the inner membrane for co-translational insertion of their hydrophobic products, so a membrane-associated assignment for the whole particle is defensible. Reactome event: 'GFM2:GTP binds 55S ribosome:mRNA:tRNA:MRRF releasing mRNA and tRNA'. Accepted. Reason: [file:human/AURKAIP1/AURKAIP1-uniprot.txt "CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix"] [PMID:23908630 "The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in"] Supporting Evidence: file:human/AURKAIP1/AURKAIP1-uniprot.txt CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix PMID:23908630 The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in |
| GO:0005743 mitochondrial inner membrane | TAS Reactome:R-HSA-5419281 | ACCEPT | Summary: Mitochondrial inner membrane (TAS). As a mitoribosomal protein, AURKAIP1 sits in the matrix-facing small subunit of the 55S mitoribosome. Mammalian mitoribosomes are tethered to the inner membrane for co-translational insertion of their hydrophobic products, so a membrane-associated assignment for the whole particle is defensible. Reactome event: 'MRRF binds 55S ribosome:mRNA:tRNA'. Accepted. Reason: [file:human/AURKAIP1/AURKAIP1-uniprot.txt "CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix"] [PMID:23908630 "The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in"] Supporting Evidence: file:human/AURKAIP1/AURKAIP1-uniprot.txt CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix PMID:23908630 The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in |
| GO:0005743 mitochondrial inner membrane | TAS Reactome:R-HSA-9937394 | ACCEPT | Summary: Mitochondrial inner membrane (TAS). As a mitoribosomal protein, AURKAIP1 sits in the matrix-facing small subunit of the 55S mitoribosome. Mammalian mitoribosomes are tethered to the inner membrane for co-translational insertion of their hydrophobic products, so a membrane-associated assignment for the whole particle is defensible. Reactome event: '55S ribosome:MTRF1 hydrolyzes peptidyl-tRNA bond and GTP is hydrolyzed'. Accepted. Reason: [file:human/AURKAIP1/AURKAIP1-uniprot.txt "CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix"] [PMID:23908630 "The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in"] Supporting Evidence: file:human/AURKAIP1/AURKAIP1-uniprot.txt CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix PMID:23908630 The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in |
| GO:0005743 mitochondrial inner membrane | TAS Reactome:R-HSA-9937412 | ACCEPT | Summary: Mitochondrial inner membrane (TAS). As a mitoribosomal protein, AURKAIP1 sits in the matrix-facing small subunit of the 55S mitoribosome. Mammalian mitoribosomes are tethered to the inner membrane for co-translational insertion of their hydrophobic products, so a membrane-associated assignment for the whole particle is defensible. Reactome event: 'Stalled 55S mitochondrial ribosome is dissociated to yield 28S subunit and 39S subunit:mRNA:peptidyl-tRNA:tRNA'. Accepted. Reason: [file:human/AURKAIP1/AURKAIP1-uniprot.txt "CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix"] [PMID:23908630 "The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in"] Supporting Evidence: file:human/AURKAIP1/AURKAIP1-uniprot.txt CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix PMID:23908630 The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in |
| GO:0005743 mitochondrial inner membrane | TAS Reactome:R-HSA-9937415 | ACCEPT | Summary: Mitochondrial inner membrane (TAS). As a mitoribosomal protein, AURKAIP1 sits in the matrix-facing small subunit of the 55S mitoribosome. Mammalian mitoribosomes are tethered to the inner membrane for co-translational insertion of their hydrophobic products, so a membrane-associated assignment for the whole particle is defensible. Reactome event: 'MTRF1 and GTP bind 55S ribosome:MT-CO1,MT-ND6 mRNA:peptidyl-tRNA at P-site:tRNA at E-site'. Accepted. Reason: [file:human/AURKAIP1/AURKAIP1-uniprot.txt "CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix"] [PMID:23908630 "The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in"] Supporting Evidence: file:human/AURKAIP1/AURKAIP1-uniprot.txt CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix PMID:23908630 The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in |
| GO:0005743 mitochondrial inner membrane | TAS Reactome:R-HSA-9937719 | ACCEPT | Summary: Mitochondrial inner membrane (TAS). As a mitoribosomal protein, AURKAIP1 sits in the matrix-facing small subunit of the 55S mitoribosome. Mammalian mitoribosomes are tethered to the inner membrane for co-translational insertion of their hydrophobic products, so a membrane-associated assignment for the whole particle is defensible. Reactome event: 'ICT1 hydrolyzes the peptidyl-tRNA bond of the nascent polypeptide'. Accepted. Reason: [file:human/AURKAIP1/AURKAIP1-uniprot.txt "CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix"] [PMID:23908630 "The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in"] Supporting Evidence: file:human/AURKAIP1/AURKAIP1-uniprot.txt CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix PMID:23908630 The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in |
| GO:0005743 mitochondrial inner membrane | TAS Reactome:R-HSA-9937724 | ACCEPT | Summary: Mitochondrial inner membrane (TAS). As a mitoribosomal protein, AURKAIP1 sits in the matrix-facing small subunit of the 55S mitoribosome. Mammalian mitoribosomes are tethered to the inner membrane for co-translational insertion of their hydrophobic products, so a membrane-associated assignment for the whole particle is defensible. Reactome event: '55S mitochondrial ribosome:non-stop mRNA binds ICT1 (MRPL58)'. Accepted. Reason: [file:human/AURKAIP1/AURKAIP1-uniprot.txt "CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix"] [PMID:23908630 "The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in"] Supporting Evidence: file:human/AURKAIP1/AURKAIP1-uniprot.txt CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix PMID:23908630 The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in |
| GO:0005759 mitochondrial matrix | IDA PMID:27184847 APEX Fingerprinting Reveals the Subcellular Localization of ... | ACCEPT | Summary: Mitochondrial matrix (IDA). As a mitoribosomal protein, AURKAIP1 sits in the matrix-facing small subunit of the 55S mitoribosome. Mammalian mitoribosomes are tethered to the inner membrane for co-translational insertion of their hydrophobic products, so a membrane-associated assignment for the whole particle is defensible. Accepted. Reason: [file:human/AURKAIP1/AURKAIP1-uniprot.txt "CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix"] [PMID:23908630 "The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in"] Supporting Evidence: file:human/AURKAIP1/AURKAIP1-uniprot.txt CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix PMID:23908630 The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in |
| GO:0005759 mitochondrial matrix | IEA GO_REF:0000044 | ACCEPT | Summary: Mitochondrial matrix (IEA). As a mitoribosomal protein, AURKAIP1 sits in the matrix-facing small subunit of the 55S mitoribosome. Mammalian mitoribosomes are tethered to the inner membrane for co-translational insertion of their hydrophobic products, so a membrane-associated assignment for the whole particle is defensible. Accepted. Reason: [file:human/AURKAIP1/AURKAIP1-uniprot.txt "CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix"] [PMID:23908630 "The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in"] Supporting Evidence: file:human/AURKAIP1/AURKAIP1-uniprot.txt CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix PMID:23908630 The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in |
| GO:0005763 mitochondrial small ribosomal subunit | NAS PMID:25838379 Ribosome. The structure of the human mitochondrial ribosome. | ACCEPT | Summary: Mitochondrial small ribosomal subunit (NAS). AURKAIP1 is MRPS38/mS38, a bona fide protein of the mitoribosome small subunit identified in purified mitoribosomes, and its knockdown impairs synthesis of mitochondrially encoded proteins. Core. Reason: [PMID:23908630 "The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in"] [PMID:23908630 "Furthermore, we have demonstrated the essential roles of CHCHD1, AURKAIP1, and CRIF1in mitochondrial protein synthesis by siRNA knock-down studies, which had significant effects on the expression of mitochondrially encoded proteins."] [PMID:23908630 "However, we observed an overall reduction in the expression of all 13 mitochondrially encoded proteins in AURKAIP1 knock-down cell lines (Figure 4C), suggesting that this protein plays a general role in mitochondrial translation that is not limited to the synthesis of cytochrome oxidase."] [file:human/AURKAIP1/AURKAIP1-uniprot.txt "CC -!- SUBUNIT: Component of the mitochondrial small ribosomal subunit (mt-"] Supporting Evidence: PMID:23908630 The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in PMID:23908630 Furthermore, we have demonstrated the essential roles of CHCHD1, AURKAIP1, and CRIF1in mitochondrial protein synthesis by siRNA knock-down studies, which had significant effects on the expression of mitochondrially encoded proteins. PMID:23908630 However, we observed an overall reduction in the expression of all 13 mitochondrially encoded proteins in AURKAIP1 knock-down cell lines (Figure 4C), suggesting that this protein plays a general role in mitochondrial translation that is not limited to the synthesis of cytochrome oxidase. file:human/AURKAIP1/AURKAIP1-uniprot.txt CC -!- SUBUNIT: Component of the mitochondrial small ribosomal subunit (mt- |
| GO:0032543 mitochondrial translation | NAS PMID:27023846 Structure and Function of the Mitochondrial Ribosome. | ACCEPT | Summary: Mitochondrial translation (NAS). AURKAIP1 is MRPS38/mS38, a bona fide protein of the mitoribosome small subunit identified in purified mitoribosomes, and its knockdown impairs synthesis of mitochondrially encoded proteins. Core. Reason: [PMID:23908630 "The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in"] [PMID:23908630 "Furthermore, we have demonstrated the essential roles of CHCHD1, AURKAIP1, and CRIF1in mitochondrial protein synthesis by siRNA knock-down studies, which had significant effects on the expression of mitochondrially encoded proteins."] [PMID:23908630 "However, we observed an overall reduction in the expression of all 13 mitochondrially encoded proteins in AURKAIP1 knock-down cell lines (Figure 4C), suggesting that this protein plays a general role in mitochondrial translation that is not limited to the synthesis of cytochrome oxidase."] [file:human/AURKAIP1/AURKAIP1-uniprot.txt "CC -!- SUBUNIT: Component of the mitochondrial small ribosomal subunit (mt-"] Supporting Evidence: PMID:23908630 The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in PMID:23908630 Furthermore, we have demonstrated the essential roles of CHCHD1, AURKAIP1, and CRIF1in mitochondrial protein synthesis by siRNA knock-down studies, which had significant effects on the expression of mitochondrially encoded proteins. PMID:23908630 However, we observed an overall reduction in the expression of all 13 mitochondrially encoded proteins in AURKAIP1 knock-down cell lines (Figure 4C), suggesting that this protein plays a general role in mitochondrial translation that is not limited to the synthesis of cytochrome oxidase. file:human/AURKAIP1/AURKAIP1-uniprot.txt CC -!- SUBUNIT: Component of the mitochondrial small ribosomal subunit (mt- |
| GO:0045862 positive regulation of proteolysis | IDA PMID:12244051 Aurora-A kinase interacting protein (AIP), a novel negative ... | KEEP AS NON CORE | Summary: Positive regulation of proteolysis (IDA). Co-overexpressing AIP with Aurora-A in NIH 3T3 and COS cells destabilized Aurora-A through proteasome-dependent degradation. Two 2007 follow-ups from the same group extend the mechanism. AURKAIP1 targets Aurora-A for proteasomal degradation independently of ubiquitin (PMID:17125467), through antizyme1, which binds Aurora-A in a complex with AURKAIP1 (PMID:17452972). The evidence is therefore mechanistic, not a single observation. These studies rely on ectopic expression in cell lines, however, and AURKAIP1's established and abundant function is in the mitoribosome. The axis is kept as non-core rather than core. Reason: [PMID:12244051 "Ectopic expression of AIP with Aurora-A in NIH 3T3 and COS cells results in the down-regulation of ectopically expressed Aurora-A protein levels, and this down-regulation is demonstrated to be the result of destabilization of Aurora-A through a proteasome-dependent protein degradation pathway."] [PMID:17125467 "In an attempt to investigate the mechanism of AURKAIP1-mediated Aurora-A degradation, we report here that AURKAIP1 targets Aurora-A for degradation in a proteasome-dependent but Ub (ubiquitin)-independent manner."] [PMID:17452972 "In an attempt to understand how AURKAIP1 promotes Aurora-A degradation through Ub-independent pathway, we demonstrate here that antizyme1 (Az1), a well-studied mediator of Ub-independent protein degradation pathway, regulates Aurora-A protein stability."] Supporting Evidence: PMID:12244051 Ectopic expression of AIP with Aurora-A in NIH 3T3 and COS cells results in the down-regulation of ectopically expressed Aurora-A protein levels, and this down-regulation is demonstrated to be the result of destabilization of Aurora-A through a proteasome-dependent protein degradation pathway. PMID:17125467 In an attempt to investigate the mechanism of AURKAIP1-mediated Aurora-A degradation, we report here that AURKAIP1 targets Aurora-A for degradation in a proteasome-dependent but Ub (ubiquitin)-independent manner. PMID:17452972 In an attempt to understand how AURKAIP1 promotes Aurora-A degradation through Ub-independent pathway, we demonstrate here that antizyme1 (Az1), a well-studied mediator of Ub-independent protein degradation pathway, regulates Aurora-A protein stability. |
| GO:0003735 structural constituent of ribosome | IDA PMID:23908630 Identification and characterization of CHCHD1, AURKAIP1, and... | NEW | Summary: Structural constituent of ribosome (NEW, IDA). AURKAIP1 is the mitoribosome small-subunit protein bS22m (formerly mS38/MRPS38), identified in purified mitoribosomes and required for mitochondrial protein synthesis. UniProt renamed it after a homolog was found in Actinomycetota ribosomes, which makes it a conserved bacterial-type SSU protein. Comparator: other mitoribosomal small-subunit proteins (MRPS34, MRPS2, MRPS5) carry this term. Reason: [PMID:23908630 "The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in"] [PMID:23908630 "Furthermore, we have demonstrated the essential roles of CHCHD1, AURKAIP1, and CRIF1in mitochondrial protein synthesis by siRNA knock-down studies, which had significant effects on the expression of mitochondrially encoded proteins."] [file:human/AURKAIP1/AURKAIP1-uniprot.txt "CC -!- SUBUNIT: Component of the mitochondrial small ribosomal subunit (mt-"] [file:human/AURKAIP1/AURKAIP1-uniprot.txt "CC comparison with ribosomes from Actinomycetota shows this protein is"] Supporting Evidence: PMID:23908630 The newly identified MRPs are MRPS37 (Coiled-coil-helix-coiled-coil-helix domain containing protein 1-CHCHD1), MRPS38 (Aurora kinase A interacting protein1, AURKAIP1), MRPS39 (Pentatricopeptide repeat-containing protein 3, PTCD3), in the small subunit and MRPL59 (CR-6 interacting factor 1, CRIF1) in PMID:23908630 Furthermore, we have demonstrated the essential roles of CHCHD1, AURKAIP1, and CRIF1in mitochondrial protein synthesis by siRNA knock-down studies, which had significant effects on the expression of mitochondrially encoded proteins. file:human/AURKAIP1/AURKAIP1-uniprot.txt CC -!- SUBUNIT: Component of the mitochondrial small ribosomal subunit (mt- file:human/AURKAIP1/AURKAIP1-uniprot.txt CC comparison with ribosomes from Actinomycetota shows this protein is |
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Download this section (compressed HTML)Q: Human AURKAIP1 knockdown reduces all mtDNA-encoded proteins, whereas yeast mS38 loss is COX-selective. Did the human function diverge, or does a general assembly defect in partial knockdowns mask an initiation-specific bias?
Q: Is there a distinct extramitochondrial (nuclear) AURKAIP1 pool, for example from alternative translation start sites, that regulates Aurora-A, or is the Aurora-A effect an overexpression artifact?
Experiment: In AURKAIP1-knockout human cells with titratable re-expression, measure mitoribosome assembly (sucrose gradients) separately from initiation (mitoribosome profiling) to locate the step bS22m acts at, and test for any MT-CO1/2/3 initiation bias at sub-saturating rescue.
Experiment: Tag endogenous AURKAIP1 and quantify its mitochondrial and nuclear pools, then test whether AURKAIP1 knockout alters endogenous Aurora-A levels independently of mitochondrial translation defects.
What is not known — curated, literature-grounded statements of the open unknowns (the inverse of core functions).
Gap: The specific mechanistic role of bS22m in human mitochondrial translation (subunit assembly, intersubunit bridging, or initiation) is unknown, as is whether any residue of the yeast mS38 mRNA-selective initiation function survives.
OPEN BIOLOGY
What is known: Established: human AURKAIP1 is a mitoribosome SSU protein. siRNA knockdown lowers all 13 mtDNA-encoded proteins without changing COI mRNA, indicating a general role not limited to cytochrome oxidase (PMID:23908630). In yeast, mS38 loss preferentially impairs COX1-3 translation initiation and also affects mitoribosome assembly (PMID:30968120). Not established: which step bS22m acts at in human mitoribosomes. Partial knockdown with steady-state pulse labelling would not resolve a modest initiation-specific bias.
Provenance (the field's own admissions):
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