BAG3 is a human BAG-family co-chaperone that coordinates Hsp70-family and small heat-shock proteins. Its BAG domain regulates Hsp70 nucleotide exchange, while its IPV motifs recruit small heat-shock proteins including HSPB8 and HSPB1. This modular organization supports chaperone-assisted folding and routes damaged clients toward selective autophagy. BAG3 also couples Hsp70-associated cargo to dynein for transport to aggresomes. It acts mainly in the cytoplasm and at muscle Z-discs, where it helps maintain protein integrity under mechanical stress. Stress-dependent nuclear localization supports HSF1 regulation, and BAG3 can modulate cell survival in specific contexts.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005634 nucleus | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: BAG3 has a stress-responsive nuclear pool in addition to its cytosolic functions. Reason: BAG3 translocates to the nucleus during heat stress in HeLa cells (PMID:26159920), consistent with an inducible nuclear pool alongside cytosolic chaperone functions. The available abstract does not resolve every nuclear subcompartment. |
| GO:0005829 cytosol | IBA GO_REF:0000033 | ACCEPT | Summary: BAG3 carries out chaperone regulation and protein quality control in the cytoplasm/cytosol. Reason: Human-cell and reconstituted experiments establish cytoplasmic chaperone regulation and cargo handling by BAG3 (PMID:27884606; PMID:30559338). These functions are consistent with the cytosolic BAG-family nucleotide-exchange step described in Reactome:R-HSA-5252079. |
| GO:0016020 membrane | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: The broad membrane location is compatible with experimentally observed sarcolemmal BAG3. Reason: Normal human muscle shows BAG3 at the Z-disc and sarcolemma (PMID:19085932). Membrane localization includes attached proteins and therefore does not require BAG3 to contain a transmembrane segment. This muscle-associated membrane pool is compatible with the broad PAINT localization. Supporting Evidence: PMID:19085932 In normal muscle, Bag3 was immunolocalized to the Z-disk (colocalizing with myotilin) and the sarcolemma. |
| GO:0000774 adenyl-nucleotide exchange factor activity | IBA GO_REF:0000033 | ACCEPT | Summary: BAG3 is an adenyl-nucleotide exchange factor for Hsp70-family chaperones. Reason: The BAG domain binds the Hsp70 nucleotide-binding domain and promotes nucleotide release. Human BAG-family comparisons and BAG3 fluorescent-nucleotide release assays support this exchange activity (PMID:24318877; PMID:27884606; PMID:30559338). Hsp70 supplies the ATPase activity. |
| GO:0010664 negative regulation of striated muscle cell apoptotic process | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: BAG3 supports striated-muscle cell survival, a context-dependent consequence of its protective functions. Reason: Human BAG3-associated myopathy includes apoptotic nuclei and severe muscle pathology (PMID:19085932). Cell survival is a context-dependent consequence of BAG3-mediated protein quality control; the disease phenotype does not establish a single autonomous apoptosis-suppressing mechanism. P209L can retain chaperone binding while disrupting productive client processing (PMID:30559338). |
| GO:0046716 muscle cell cellular homeostasis | IBA GO_REF:0000033 | ACCEPT | Summary: BAG3-mediated chaperone coordination maintains muscle protein homeostasis. Reason: BAG3 coordinates selective autophagy required for muscle maintenance (PMID:20060297). Normal human Z-disc localization and myofibrillar pathology (PMID:19085932), together with defective chaperone cooperation by myopathy mutants (PMID:30559338), support a direct scaffold and chaperone-regulatory contribution to muscle protein homeostasis. |
| GO:0050821 protein stabilization | IBA GO_REF:0000033 | ACCEPT | Summary: Protein stabilization is compatible with BAG3-dependent chaperone coordination and prevention of aggregation. Reason: Protein stabilization includes maintaining protein integrity and preventing aggregation. BAG3-dependent chaperone cooperation can support these outcomes even though other clients are routed for degradation. Functional refolding experiments provide independent support (PMID:27884606; PMID:30559338); the detailed assay underlying the PMID:18006506 IDA was unavailable in the accessible abstract. |
| GO:0051087 protein-folding chaperone binding | IBA GO_REF:0000033 | ACCEPT | Summary: Binding protein-folding chaperones is a central BAG3 molecular function. Reason: Reconstituted experiments map Hsp70 binding to the BAG domain and small heat-shock protein binding to the IPV motifs (PMID:27884606). These contacts coordinate chaperone functions, although binding alone does not guarantee productive client processing (PMID:30559338). |
| GO:0000045 autophagosome assembly | IEA GO_REF:0000107 | ACCEPT | Summary: BAG3 participates in autophagosome assembly through the CASA scaffold machinery. Reason: BAG3-SYNPO2 coupling connects the CASA machinery to autophagosome formation (PMID:23434281). Human-cell experiments also show BAG3-dependent autophagic flux (PMID:19229298). BAG3 coordinates this machinery; the membrane-fusion proteins execute fusion. |
| GO:0001725 stress fiber | IEA GO_REF:0000107 | UNDECIDED | Summary: The rat-derived stress-fiber location is plausible but the primary localization experiment remains unread. Reason: The rat BAG3 localization assertion derives from the PMID:23434281 IDA record. Its accessible abstract describes force-dependent CASA but does not expose the specific stress-fiber localization experiment. Localization to Z-discs in muscle does not exclude an actin stress-fiber pool; the unavailable full experiment is needed to resolve this location. |
| GO:0021510 spinal cord development | IEA GO_REF:0000107 | UNDECIDED | Summary: The available developmental-expression evidence does not resolve the spinal-cord-development assignment. Reason: PMID:19415333 reports BAG3/Bis expression in radial glia of developing rat brainstem and spinal cord. Only the abstract and official bibliographic record were inspected; the complete experimental paper was not. The available account does not allow the specific developmental contribution behind the inferred annotation to be adjudicated, so the assignment remains UNDECIDED. Supporting Evidence: PMID:19415333 analyzed the expression of Bis in the rat brainstem and cervical spinal cord during development by using immunohistochemistry. |
| GO:0030018 Z disc | IEA GO_REF:0000107 | ACCEPT | Summary: BAG3 localizes to the muscle Z-disc. Reason: Normal human muscle BAG3 colocalizes with myotilin at the Z-disc (PMID:19085932), independently supporting the mouse-derived location. BAG3-dependent chaperone coordination contributes to maintenance of this compartment (PMID:20060297). |
| GO:0043066 negative regulation of apoptotic process | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: BAG3 can suppress apoptosis in specific cellular contexts. Reason: Human BIS/BAG3 has weak anti-apoptotic activity alone and synergizes with BCL2 against BAX- and Fas-triggered cell death (PMID:10597216). This supports a context-dependent survival role. |
| GO:0044877 protein-containing complex binding | IEA GO_REF:0000120 | MODIFY | Summary: The broad complex-binding assertion can be expressed more informatively as BAG3 adaptor activity. Reason: BAG3 simultaneously couples Hsp70 and small heat-shock proteins in reconstituted complexes (PMID:27884606) and links Hsp70-associated cargo to dynein (PMID:21252941). Protein-macromolecule adaptor activity expresses this coordinated function more precisely than general complex binding. The same adaptor activity is independently represented by an accepted IDA annotation; this replacement refines the broad source assertion without proposing additional functional coverage. Proposed replacements: protein-macromolecule adaptor activity |
| GO:0046716 muscle cell cellular homeostasis | IEA GO_REF:0000120 | ACCEPT | Summary: BAG3-mediated chaperone coordination maintains muscle protein homeostasis. Reason: BAG3 coordinates selective autophagy required for muscle maintenance (PMID:20060297). Normal human Z-disc localization and myofibrillar pathology (PMID:19085932), together with defective chaperone cooperation by myopathy mutants (PMID:30559338), support a direct scaffold and chaperone-regulatory contribution to muscle protein homeostasis. |
| GO:0061684 chaperone-mediated autophagy | IEA GO_REF:0000107 | ACCEPT | Summary: BAG3 participates in chaperone-assisted selective autophagy, which the current GO term explicitly includes. Reason: The current GO:0061684 record lists chaperone-assisted selective autophagy (CASA) as a synonym and cites PMID:23434281 in its definition. BAG3 coordinates Hsc70/HSPB8-dependent handling of damaged filamin and couples this machinery to SYNPO2-dependent autophagosome formation (PMID:20060297; PMID:23434281), supporting participation in CASA under the current term scope. CASA is biologically distinct from canonical chaperone-mediated direct lysosomal translocation; retaining this ontology assignment does not attribute that latter mechanism to BAG3. |
| GO:1903748 negative regulation of protein localization to mitochondrion | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: BAG3-dependent cytosolic retention of BAX supports a specific non-core mitochondrial-localization regulatory role. Reason: The separately inspected official PMC3124067 Methods and BAX Results identify rat C6 glioma cells as the system in which BAG3 depletion redistributes BAX from cytosol to mitochondria (PMID:21561597). The cached abstract independently reports BAX retention but does not name C6. These results support a context-specific role in restricting BAX mitochondrial localization. Human tumor staining and human-cell survival experiments in the same paper are distinct from the rat mechanistic assays. Supporting Evidence: PMID:21561597 formation of a complex of BAG3, HSP70, and BAX that prevents BAX translocation to mitochondria |
| GO:0005515 protein binding | IPI PMID:16189514 Towards a proteome-scale map of the human protein-protein in... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q15038. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q15038, curated from PMID:16189514. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:19229298 Protein quality control during aging involves recruitment of... | KEEP AS NON CORE | Summary: BAG3 associates with SQSTM1/p62 during autophagic protein quality control. Reason: PMID:19229298 reports reciprocal coimmunoprecipitation of BAG3 and SQSTM1 at endogenous and increased BAG3 levels, together with colocalization in human I90 fibroblasts and cooperative autophagic handling. These observations support the association within the broader quality-control machinery but do not distinguish direct from indirect binding. The generic interaction is retained alongside the separately described BAG3 chaperone and cargo-handling activities. |
| GO:0005515 protein binding | IPI PMID:21044950 Genome-wide YFP fluorescence complementation screen identifi... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q9BSI4. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q9BSI4, curated from PMID:21044950. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:21516116 Next-generation sequencing to generate interactome datasets. | MODIFY | Summary: BAG3 binds the protein-folding chaperone HSPB8. Reason: Direct BAG3-HSPB8 binding and ternary-complex assembly with Hsp70 are demonstrated in PMID:27884606. This supports a chaperone-specific refinement of the same partner interaction. HSPB8 variants can alter this association (PMID:28144995). Proposed replacements: protein-folding chaperone binding |
| GO:0005515 protein binding | IPI PMID:24510904 Unbiased screen for interactors of leucine-rich repeat kinas... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q5S007. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q5S007, curated from PMID:24510904. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:25036637 A quantitative chaperone interaction network reveals the arc... | MODIFY | Summary: BAG3 binds HSPB1/Hsp27 through its IPV motifs. Reason: GOA identifies the experimental interaction partner as UniProtKB:P04792 (HSPB1/Hsp27). The original pair-specific supplementary result was not independently inspected here. Independent PMID:27884606 directly demonstrates HSPB1 binding, IPV-motif dependence and remodeling of Hsp27 oligomers, supporting the more specific Hsp27-binding term for this same partner. Proposed replacements: Hsp27 protein binding Supporting Evidence: PMID:27884606 BAG3 uses both of its IPV motifs to interact with sHsps |
| GO:0005515 protein binding | IPI PMID:25277244 The functional landscape of Hsp27 reveals new cellular proce... | MODIFY | Summary: BAG3 binds HSPB1/Hsp27 through its IPV motifs. Reason: GOA identifies the experimental interaction partner as UniProtKB:P04792 (HSPB1/Hsp27). The original pair-specific supplementary result was not independently inspected here. Independent PMID:27884606 directly demonstrates HSPB1 binding, IPV-motif dependence and remodeling of Hsp27 oligomers, supporting the more specific Hsp27-binding term for this same partner. Proposed replacements: Hsp27 protein binding |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:O76083. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:O76083, curated from PMID:25416956. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:26496610 A human interactome in three quantitative dimensions organiz... | MODIFY | Summary: BAG3 binds HSPB1/Hsp27 through its IPV motifs. Reason: GOA identifies the experimental interaction partner as UniProtKB:P04792 (HSPB1/Hsp27). The original pair-specific supplementary result was not independently inspected here. Independent PMID:27884606 directly demonstrates HSPB1 binding, IPV-motif dependence and remodeling of Hsp27 oligomers, supporting the more specific Hsp27-binding term for this same partner. Proposed replacements: Hsp27 protein binding |
| GO:0005515 protein binding | IPI PMID:27880917 Phenotypic and Interaction Profiling of the Human Phosphatas... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:O00167-2. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:O00167-2, curated from PMID:27880917. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:28514442 Architecture of the human interactome defines protein commun... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q9NR12. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q9NR12, curated from PMID:28514442. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:31515488 Extensive disruption of protein interactions by genetic vari... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q99732. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q99732, curated from PMID:31515488. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:A0A087WSW0. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:A0A087WSW0, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32707033 Kinase Interaction Network Expands Functional and Disease Ro... | MODIFY | Summary: BAG3 binds the protein-folding chaperone HSPB8. Reason: Direct BAG3-HSPB8 binding and ternary-complex assembly with Hsp70 are demonstrated in PMID:27884606. This supports a chaperone-specific refinement of the same partner interaction. HSPB8 variants can alter this association (PMID:28144995). Proposed replacements: protein-folding chaperone binding |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:O75446. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:O75446, curated from PMID:32814053. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:P02489. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:P02489, curated from PMID:33961781. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005634 nucleus | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: BAG3 has a stress-responsive nuclear pool in addition to its cytosolic functions. Reason: BAG3 translocates to the nucleus during heat stress in HeLa cells (PMID:26159920), consistent with an inducible nuclear pool alongside cytosolic chaperone functions. The available abstract does not resolve every nuclear subcompartment. |
| GO:0005737 cytoplasm | IEA GO_REF:0000120 | ACCEPT | Summary: BAG3 carries out chaperone regulation and protein quality control in the cytoplasm/cytosol. Reason: Human-cell and reconstituted experiments establish cytoplasmic chaperone regulation and cargo handling by BAG3 (PMID:27884606; PMID:30559338). These functions are consistent with the cytosolic BAG-family nucleotide-exchange step described in Reactome:R-HSA-5252079. |
| GO:0006457 protein folding | IEA GO_REF:0000117 | ACCEPT | Summary: BAG3 regulates chaperone-assisted protein folding. Reason: BAG3 regulates Hsp70-dependent luciferase refolding in reconstituted assays (PMID:27884606; PMID:30559338). The outcome depends on chaperone identity and relative concentrations: suitable ratios promote folding, whereas excess BAG3 can inhibit it. This is cooperation with the chaperone cycle rather than autonomous BAG3 holdase activity. |
| GO:0010664 negative regulation of striated muscle cell apoptotic process | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: BAG3 supports striated-muscle cell survival, a context-dependent consequence of its protective functions. Reason: Human BAG3-associated myopathy includes apoptotic nuclei and severe muscle pathology (PMID:19085932). Cell survival is a context-dependent consequence of BAG3-mediated protein quality control; the disease phenotype does not establish a single autonomous apoptosis-suppressing mechanism. P209L can retain chaperone binding while disrupting productive client processing (PMID:30559338). |
| GO:0050821 protein stabilization | IEA GO_REF:0000117 | ACCEPT | Summary: Protein stabilization is compatible with BAG3-dependent chaperone coordination and prevention of aggregation. Reason: Protein stabilization includes maintaining protein integrity and preventing aggregation. BAG3-dependent chaperone cooperation can support these outcomes even though other clients are routed for degradation. Functional refolding experiments provide independent support (PMID:27884606; PMID:30559338); the detailed assay underlying the PMID:18006506 IDA was unavailable in the accessible abstract. |
| GO:0051087 protein-folding chaperone binding | IEA GO_REF:0000002 | ACCEPT | Summary: Binding protein-folding chaperones is a central BAG3 molecular function. Reason: Reconstituted experiments map Hsp70 binding to the BAG domain and small heat-shock protein binding to the IPV motifs (PMID:27884606). These contacts coordinate chaperone functions, although binding alone does not guarantee productive client processing (PMID:30559338). |
| GO:0097191 extrinsic apoptotic signaling pathway | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: BAG3 modulates extrinsic apoptotic signaling in an anti-apoptotic context. Reason: BAG3 and BCL2 cooperate in protection against Fas-mediated cell death (PMID:10597216). The extrinsic apoptotic pathway association therefore represents regulation of survival in this setting, without implying that BAG3 is a death receptor or promotes apoptosis. |
| GO:0005654 nucleoplasm | IDA GO_REF:0000052 | KEEP AS NON CORE | Summary: BAG3 has a stress-responsive nuclear pool in addition to its cytosolic functions. Reason: BAG3 translocates to the nucleus during heat stress in HeLa cells (PMID:26159920), consistent with an inducible nuclear pool alongside cytosolic chaperone functions. The available abstract does not resolve every nuclear subcompartment. |
| GO:0005829 cytosol | IDA GO_REF:0000052 | ACCEPT | Summary: BAG3 carries out chaperone regulation and protein quality control in the cytoplasm/cytosol. Reason: Human-cell and reconstituted experiments establish cytoplasmic chaperone regulation and cargo handling by BAG3 (PMID:27884606; PMID:30559338). These functions are consistent with the cytosolic BAG-family nucleotide-exchange step described in Reactome:R-HSA-5252079. |
| GO:0000774 adenyl-nucleotide exchange factor activity | IDA PMID:30559338 Myopathy associated BAG3 mutations lead to protein aggregati... | ACCEPT | Summary: BAG3 is an adenyl-nucleotide exchange factor for Hsp70-family chaperones. Reason: The BAG domain binds the Hsp70 nucleotide-binding domain and promotes nucleotide release. Human BAG-family comparisons and BAG3 fluorescent-nucleotide release assays support this exchange activity (PMID:24318877; PMID:27884606; PMID:30559338). Hsp70 supplies the ATPase activity. |
| GO:0006457 protein folding | IDA PMID:30559338 Myopathy associated BAG3 mutations lead to protein aggregati... | ACCEPT | Summary: BAG3 regulates chaperone-assisted protein folding. Reason: BAG3 regulates Hsp70-dependent luciferase refolding in reconstituted assays (PMID:27884606; PMID:30559338). The outcome depends on chaperone identity and relative concentrations: suitable ratios promote folding, whereas excess BAG3 can inhibit it. This is cooperation with the chaperone cycle rather than autonomous BAG3 holdase activity. |
| GO:0030674 protein-macromolecule adaptor activity | IDA PMID:27884606 BAG3 Is a Modular, Scaffolding Protein that physically Links... | ACCEPT | Summary: BAG3 is a modular adaptor coupling Hsp70 and small heat-shock proteins. Reason: Simultaneous binding, domain-dependence and ternary-complex experiments show that BAG3 brings Hsp70 and small heat-shock proteins together (PMID:27884606). This coupling supports coordinated chaperone function; endogenous stoichiometry need not be identical for every small-HSP complex. |
| GO:0051087 protein-folding chaperone binding | IDA PMID:27884606 BAG3 Is a Modular, Scaffolding Protein that physically Links... | ACCEPT | Summary: Binding protein-folding chaperones is a central BAG3 molecular function. Reason: Reconstituted experiments map Hsp70 binding to the BAG domain and small heat-shock protein binding to the IPV motifs (PMID:27884606). These contacts coordinate chaperone functions, although binding alone does not guarantee productive client processing (PMID:30559338). |
| GO:0051087 protein-folding chaperone binding | IDA PMID:30559338 Myopathy associated BAG3 mutations lead to protein aggregati... | ACCEPT | Summary: Binding protein-folding chaperones is a central BAG3 molecular function. Reason: Reconstituted experiments map Hsp70 binding to the BAG domain and small heat-shock protein binding to the IPV motifs (PMID:27884606). These contacts coordinate chaperone functions, although binding alone does not guarantee productive client processing (PMID:30559338). |
| GO:0140597 protein carrier activity | TAS PMID:21681022 BAG3 and friends: co-chaperones in selective autophagy durin... | ACCEPT | Summary: BAG3 carries Hsp70-associated protein cargo toward aggresomes. Reason: BAG3 associates with Hsp70-bound misfolded proteins, couples them to dynein, and travels with them along microtubules to aggresomes (PMID:21252941). This supports the existing protein-carrier assertion. Client recognition is mediated by Hsp70; the experiments do not establish independent direct BAG3 contact with every misfolded client. |
| GO:0098840 protein transport along microtubule | TAS PMID:21681022 BAG3 and friends: co-chaperones in selective autophagy durin... | ACCEPT | Summary: BAG3 couples Hsp70-associated clients to dynein-dependent aggresome transport and assembly. Reason: BAG3 associates with dynein intermediate chain and Hsp70-bound clients, then accumulates with the cargo in aggresomes through microtubule-dependent transport (PMID:21252941). Dynein supplies movement while BAG3 supplies cargo coupling and accompanies the complex. |
| GO:1905337 positive regulation of aggrephagy | IMP PMID:18006506 HspB8 chaperone activity toward poly(Q)-containing proteins ... | ACCEPT | Summary: BAG3 promotes autophagic clearance of aggregation-prone proteins. Reason: BAG3 promotes HSPB8-dependent clearance of aggregation-prone polyglutamine material (PMID:18006506). Primary cell studies also connect BAG3-mediated client handling to aggresome targeting and autophagic disposal (PMID:21252941; PMID:19229298). This is a regulatory and cargo-coordination role, with degradation performed by the lysosome. Supporting Evidence: PMID:18006506 Bag3 overexpression resulted in the accelerated degradation of Htt43Q |
| GO:0005515 protein binding | IPI PMID:23434281 Cellular mechanotransduction relies on tension-induced and c... | KEEP AS NON CORE | Summary: The source partner Q8TEU7 is RAPGEF6. Reason: This IPI row retains the interaction with RAPGEF6 (Q8TEU7). The BAG3-SYNPO2 adaptor mechanism in PMID:23434281 concerns the distinct partner Q9UMS6; it does not establish that mechanism for RAPGEF6. The individual RAPGEF6 experiment was unavailable for independent assessment. |
| GO:0010664 negative regulation of striated muscle cell apoptotic process | IMP PMID:19085932 Mutation in BAG3 causes severe dominant childhood muscular d... | KEEP AS NON CORE | Summary: BAG3 supports striated-muscle cell survival, a context-dependent consequence of its protective functions. Reason: Human BAG3-associated myopathy includes apoptotic nuclei and severe muscle pathology (PMID:19085932). Cell survival is a context-dependent consequence of BAG3-mediated protein quality control; the disease phenotype does not establish a single autonomous apoptosis-suppressing mechanism. P209L can retain chaperone binding while disrupting productive client processing (PMID:30559338). |
| GO:0016235 aggresome | TAS PMID:21681022 BAG3 and friends: co-chaperones in selective autophagy durin... | ACCEPT | Summary: BAG3 couples Hsp70-associated clients to dynein-dependent aggresome transport and assembly. Reason: BAG3 associates with dynein intermediate chain and Hsp70-bound clients, then accumulates with the cargo in aggresomes through microtubule-dependent transport (PMID:21252941). Dynein supplies movement while BAG3 supplies cargo coupling and accompanies the complex. |
| GO:0034620 cellular response to unfolded protein | IMP PMID:18006506 HspB8 chaperone activity toward poly(Q)-containing proteins ... | KEEP AS NON CORE | Summary: BAG3 participates in cellular responses to proteotoxic stress. Reason: BAG3 coordinates handling of aggregation-prone proteins and responds to heat stress through HSF1-associated regulation (PMID:18006506; PMID:26159920). The broad stress-response terms describe the contexts in which its more specific chaperone and cargo-handling functions operate. |
| GO:0045505 dynein intermediate chain binding | NAS PMID:21252941 BAG3 mediates chaperone-based aggresome-targeting and select... | ACCEPT | Summary: BAG3 couples Hsp70-associated clients to dynein-dependent aggresome transport and assembly. Reason: GST pull-down and coimmunoprecipitation experiments detect dynein intermediate chain in BAG3-associated complexes, including endogenous complexes (PMID:21252941). This supports the specific association used in aggresome targeting; the available results do not establish an isolated purified binary interaction. Supporting Evidence: PMID:21252941 endogenous DIC was detected in immunoprecipitated BAG3 complexes |
| GO:0046716 muscle cell cellular homeostasis | IMP PMID:19085932 Mutation in BAG3 causes severe dominant childhood muscular d... | ACCEPT | Summary: BAG3-mediated chaperone coordination maintains muscle protein homeostasis. Reason: BAG3 coordinates selective autophagy required for muscle maintenance (PMID:20060297). Normal human Z-disc localization and myofibrillar pathology (PMID:19085932), together with defective chaperone cooperation by myopathy mutants (PMID:30559338), support a direct scaffold and chaperone-regulatory contribution to muscle protein homeostasis. |
| GO:0050821 protein stabilization | IDA PMID:18006506 HspB8 chaperone activity toward poly(Q)-containing proteins ... | ACCEPT | Summary: Protein stabilization is compatible with BAG3-dependent chaperone coordination and prevention of aggregation. Reason: Protein stabilization includes maintaining protein integrity and preventing aggregation. BAG3-dependent chaperone cooperation can support these outcomes even though other clients are routed for degradation. Functional refolding experiments provide independent support (PMID:27884606; PMID:30559338); the detailed assay underlying the PMID:18006506 IDA was unavailable in the accessible abstract. |
| GO:0051087 protein-folding chaperone binding | IPI PMID:18006506 HspB8 chaperone activity toward poly(Q)-containing proteins ... | ACCEPT | Summary: Binding protein-folding chaperones is a central BAG3 molecular function. Reason: Reconstituted experiments map Hsp70 binding to the BAG domain and small heat-shock protein binding to the IPV motifs (PMID:27884606). These contacts coordinate chaperone functions, although binding alone does not guarantee productive client processing (PMID:30559338). |
| GO:0070842 aggresome assembly | TAS PMID:21681022 BAG3 and friends: co-chaperones in selective autophagy durin... | ACCEPT | Summary: BAG3 couples Hsp70-associated clients to dynein-dependent aggresome transport and assembly. Reason: BAG3 associates with dynein intermediate chain and Hsp70-bound clients, then accumulates with the cargo in aggresomes through microtubule-dependent transport (PMID:21252941). Dynein supplies movement while BAG3 supplies cargo coupling and accompanies the complex. |
| GO:0101031 protein folding chaperone complex | IDA PMID:18006506 HspB8 chaperone activity toward poly(Q)-containing proteins ... | ACCEPT | Summary: BAG3 participates in a protein-folding chaperone complex. Reason: The functional HSPB8-BAG3 association (PMID:18006506) and reconstituted Hsp70-BAG3-small-HSP complexes (PMID:27884606) support membership in protein-folding chaperone complexes. Their composition and stoichiometry can depend on the client and small-HSP partner. |
| GO:0045296 cadherin binding | HDA PMID:25468996 E-cadherin interactome complexity and robustness resolved by... | UNDECIDED | Summary: The specific cadherin-binding interpretation requires BAG3-level evidence beyond the accessible proximity-proteomics description. Reason: PMID:25468996 combines E-cadherin proximity biotinylation with quantitative proteomics and localization of selected candidates. Proximity alone does not resolve specific cadherin binding. The individual BAG3 supplementary result was not independently inspected, so the molecular-function interpretation of this HDA assertion remains uncertain. |
| GO:0042307 positive regulation of protein import into nucleus | IMP PMID:26159920 BAG3 affects the nucleocytoplasmic shuttling of HSF1 upon he... | KEEP AS NON CORE | Summary: BAG3 regulates HSF1 nucleocytoplasmic shuttling during heat stress and recovery. Reason: BAG3 associates with HSF1 and contributes to nuclear HSF1 accumulation during heat stress. During recovery, BAG3 overexpression enhances HSF1 export to the cytoplasm (PMID:26159920). The different directions reflect different phases of this regulated response, without establishing BAG3 as a general nuclear transport receptor. |
| GO:0005515 protein binding | IPI PMID:28144995 Axonal Neuropathies due to Mutations in Small Heat Shock Pro... | MODIFY | Summary: BAG3 binds the protein-folding chaperone HSPB8. Reason: Direct BAG3-HSPB8 binding and ternary-complex assembly with Hsp70 are demonstrated in PMID:27884606. This supports a chaperone-specific refinement of the same partner interaction. HSPB8 variants can alter this association (PMID:28144995). Proposed replacements: protein-folding chaperone binding |
| GO:0000774 adenyl-nucleotide exchange factor activity | IDA PMID:24318877 Binding of human nucleotide exchange factors to heat shock p... | ACCEPT | Summary: BAG3 is an adenyl-nucleotide exchange factor for Hsp70-family chaperones. Reason: The BAG domain binds the Hsp70 nucleotide-binding domain and promotes nucleotide release. Human BAG-family comparisons and BAG3 fluorescent-nucleotide release assays support this exchange activity (PMID:24318877; PMID:27884606; PMID:30559338). Hsp70 supplies the ATPase activity. |
| GO:0005515 protein binding | IPI PMID:24318877 Binding of human nucleotide exchange factors to heat shock p... | MODIFY | Summary: BAG3 binds the Hsp70-family partner through its BAG domain. Reason: The source partner P0DMV8 is HSPA1A. PMID:24318877 directly examines human BAG3-HSPA1A binding, supporting Hsp70 protein binding. Nucleotide exchange is supported by separate activity measurements, rather than inferred solely from this IPI interaction. Proposed replacements: Hsp70 protein binding |
| GO:0005515 protein binding | IPI PMID:26159920 BAG3 affects the nucleocytoplasmic shuttling of HSF1 upon he... | MODIFY | Summary: BAG3 binds the DNA-binding transcription factor HSF1. Reason: PMID:26159920 reports BAG3-HSF1 association through the BAG domain during the heat-stress response. This supports transcription-factor binding; it does not assign DNA-binding activity to BAG3. Proposed replacements: DNA-binding transcription factor binding Supporting Evidence: PMID:26159920 BAG3 interacts with HSF1 via its BAG domain |
| GO:0005634 nucleus | IDA PMID:26159920 BAG3 affects the nucleocytoplasmic shuttling of HSF1 upon he... | KEEP AS NON CORE | Summary: BAG3 has a stress-responsive nuclear pool in addition to its cytosolic functions. Reason: BAG3 translocates to the nucleus during heat stress in HeLa cells (PMID:26159920), consistent with an inducible nuclear pool alongside cytosolic chaperone functions. The available abstract does not resolve every nuclear subcompartment. Supporting Evidence: PMID:26159920 BAG3 rapidly translocalized to the nucleus upon heat stress |
| GO:0005737 cytoplasm | IDA PMID:26159920 BAG3 affects the nucleocytoplasmic shuttling of HSF1 upon he... | ACCEPT | Summary: BAG3 carries out chaperone regulation and protein quality control in the cytoplasm/cytosol. Reason: Human-cell and reconstituted experiments establish cytoplasmic chaperone regulation and cargo handling by BAG3 (PMID:27884606; PMID:30559338). These functions are consistent with the cytosolic BAG-family nucleotide-exchange step described in Reactome:R-HSA-5252079. |
| GO:0034605 cellular response to heat | IDA PMID:26159920 BAG3 affects the nucleocytoplasmic shuttling of HSF1 upon he... | KEEP AS NON CORE | Summary: BAG3 participates in cellular responses to proteotoxic stress. Reason: BAG3 coordinates handling of aggregation-prone proteins and responds to heat stress through HSF1-associated regulation (PMID:18006506; PMID:26159920). The broad stress-response terms describe the contexts in which its more specific chaperone and cargo-handling functions operate. |
| GO:0046827 positive regulation of protein export from nucleus | IMP PMID:26159920 BAG3 affects the nucleocytoplasmic shuttling of HSF1 upon he... | KEEP AS NON CORE | Summary: BAG3 regulates HSF1 nucleocytoplasmic shuttling during heat stress and recovery. Reason: BAG3 associates with HSF1 and contributes to nuclear HSF1 accumulation during heat stress. During recovery, BAG3 overexpression enhances HSF1 export to the cytoplasm (PMID:26159920). The different directions reflect different phases of this regulated response, without establishing BAG3 as a general nuclear transport receptor. |
| GO:0000774 adenyl-nucleotide exchange factor activity | IDA PMID:20060297 Chaperone-assisted selective autophagy is essential for musc... | ACCEPT | Summary: BAG3 is an adenyl-nucleotide exchange factor for Hsp70-family chaperones. Reason: The BAG domain binds the Hsp70 nucleotide-binding domain and promotes nucleotide release. Human BAG-family comparisons and BAG3 fluorescent-nucleotide release assays support this exchange activity (PMID:24318877; PMID:27884606; PMID:30559338). Hsp70 supplies the ATPase activity. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5252079 | ACCEPT | Summary: BAG3 carries out chaperone regulation and protein quality control in the cytoplasm/cytosol. Reason: Human-cell and reconstituted experiments establish cytoplasmic chaperone regulation and cargo handling by BAG3 (PMID:27884606; PMID:30559338). These functions are consistent with the cytosolic BAG-family nucleotide-exchange step described in Reactome:R-HSA-5252079. |
| GO:0008625 extrinsic apoptotic signaling pathway via death domain receptors | IDA PMID:10597216 Bis, a Bcl-2-binding protein that synergizes with Bcl-2 in p... | KEEP AS NON CORE | Summary: BAG3 modulates extrinsic apoptotic signaling in an anti-apoptotic context. Reason: BAG3 and BCL2 cooperate in protection against Fas-mediated cell death (PMID:10597216). The extrinsic apoptotic pathway association therefore represents regulation of survival in this setting, without implying that BAG3 is a death receptor or promotes apoptosis. |
| GO:0005515 protein binding | IPI PMID:22366786 Mutations affecting the cytoplasmic functions of the co-chap... | MODIFY | Summary: BAG3 associates with the chaperone DNAJB6b. Reason: Human DNAJB6b and BAG3 constructs coimmunoprecipitate in COS-1 cells in PMID:22366786, with HSPB8 and STUB1 also associated. These results support chaperone binding in a protein-quality-control context; they do not establish purified direct binary binding. Proposed replacements: protein-folding chaperone binding Supporting Evidence: PMID:22366786 observed interaction of DNAJB6b with BAG3, HSPB8, and STUB1 |
| GO:0006457 protein folding | NAS PMID:9873016 An evolutionarily conserved family of Hsp70/Hsc70 molecular ... | ACCEPT | Summary: BAG3 regulates chaperone-assisted protein folding. Reason: BAG3 regulates Hsp70-dependent luciferase refolding in reconstituted assays (PMID:27884606; PMID:30559338). The outcome depends on chaperone identity and relative concentrations: suitable ratios promote folding, whereas excess BAG3 can inhibit it. This is cooperation with the chaperone cycle rather than autonomous BAG3 holdase activity. |
| GO:0043066 negative regulation of apoptotic process | NAS PMID:10597216 Bis, a Bcl-2-binding protein that synergizes with Bcl-2 in p... | KEEP AS NON CORE | Summary: BAG3 can suppress apoptosis in specific cellular contexts. Reason: Human BIS/BAG3 has weak anti-apoptotic activity alone and synergizes with BCL2 against BAX- and Fas-triggered cell death (PMID:10597216). This supports a context-dependent survival role. |
| GO:0005829 cytosol | IDA PMID:10597216 Bis, a Bcl-2-binding protein that synergizes with Bcl-2 in p... | ACCEPT | Summary: BAG3 carries out chaperone regulation and protein quality control in the cytoplasm/cytosol. Reason: Human-cell and reconstituted experiments establish cytoplasmic chaperone regulation and cargo handling by BAG3 (PMID:27884606; PMID:30559338). These functions are consistent with the cytosolic BAG-family nucleotide-exchange step described in Reactome:R-HSA-5252079. |
| GO:0005515 protein binding | IPI PMID:16189514 Towards a proteome-scale map of the human protein-protein in... | MODIFY | Summary: BAG3 binds the protein-folding chaperone HSPB8. Reason: Direct BAG3-HSPB8 binding and ternary-complex assembly with Hsp70 are demonstrated in PMID:27884606. This supports a chaperone-specific refinement of the same partner interaction. HSPB8 variants can alter this association (PMID:28144995). Proposed replacements: protein-folding chaperone binding |
| GO:0005515 protein binding | IPI PMID:23434281 Cellular mechanotransduction relies on tension-induced and c... | MODIFY | Summary: BAG3-SYNPO2 coupling coordinates CASA with autophagosome formation. Reason: The source partner Q9UMS6 is SYNPO2. The mechanism described in PMID:23434281 connects BAG3-SYNPO2 interaction to cooperation between CASA and membrane machinery for autophagosome formation, supporting adaptor activity. Proposed replacements: protein-macromolecule adaptor activity |
| GO:0005515 protein binding | IPI PMID:24318877 Binding of human nucleotide exchange factors to heat shock p... | MODIFY | Summary: BAG3 binds the Hsp70-family partner through its BAG domain. Reason: The source partner belongs to the Hsp70 family. Human HSPA1A-BAG3 association is directly examined in PMID:24318877, and HSPA8/Hsc70-based experiments provide further mechanistic support (PMID:27884606). The retained HSPA1B interaction is curator-supported; its individual assay is not exposed by the available abstract. This binding refinement is distinct from the separately supported nucleotide-exchange activity. Proposed replacements: Hsp70 protein binding |
| GO:0005515 protein binding | IPI PMID:24318877 Binding of human nucleotide exchange factors to heat shock p... | MODIFY | Summary: BAG3 binds the Hsp70-family partner through its BAG domain. Reason: The source partner P11142 is HSPA8/Hsc70. BAG-domain association and reconstituted Hsc70-dependent functions are supported by PMID:27884606 and PMID:30559338, providing a specific Hsp70-binding refinement. Proposed replacements: Hsp70 protein binding |
| GO:0005515 protein binding | IPI PMID:25036637 A quantitative chaperone interaction network reveals the arc... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q15773. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q15773, curated from PMID:25036637. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:25036637 A quantitative chaperone interaction network reveals the arc... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q969T9. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q969T9, curated from PMID:25036637. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:25036637 A quantitative chaperone interaction network reveals the arc... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q96BE0. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q96BE0, curated from PMID:25036637. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:25036637 A quantitative chaperone interaction network reveals the arc... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q9NR12. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q9NR12, curated from PMID:25036637. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:25036637 A quantitative chaperone interaction network reveals the arc... | MODIFY | Summary: BAG3 binds the protein-folding chaperone HSPB8. Reason: Direct BAG3-HSPB8 binding and ternary-complex assembly with Hsp70 are demonstrated in PMID:27884606. This supports a chaperone-specific refinement of the same partner interaction. HSPB8 variants can alter this association (PMID:28144995). Proposed replacements: protein-folding chaperone binding |
| GO:0005515 protein binding | IPI PMID:25036637 A quantitative chaperone interaction network reveals the arc... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q9UNE7. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q9UNE7, curated from PMID:25036637. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q15038. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q15038, curated from PMID:25416956. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q15427. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q15427, curated from PMID:25416956. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q96B67. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q96B67, curated from PMID:25416956. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q96IS6. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q96IS6, curated from PMID:25416956. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q99732. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q99732, curated from PMID:25416956. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q9Y5V3. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q9Y5V3, curated from PMID:25416956. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:26496610 A human interactome in three quantitative dimensions organiz... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q15773. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q15773, curated from PMID:26496610. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:26496610 A human interactome in three quantitative dimensions organiz... | MODIFY | Summary: BAG3 binds the protein-folding chaperone HSPB8. Reason: Direct BAG3-HSPB8 binding and ternary-complex assembly with Hsp70 are demonstrated in PMID:27884606. This supports a chaperone-specific refinement of the same partner interaction. HSPB8 variants can alter this association (PMID:28144995). Proposed replacements: protein-folding chaperone binding |
| GO:0005515 protein binding | IPI PMID:26496610 A human interactome in three quantitative dimensions organiz... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q9UNE7. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q9UNE7, curated from PMID:26496610. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:28514442 Architecture of the human interactome defines protein commun... | MODIFY | Summary: BAG3 binds the protein-folding chaperone HSPB8. Reason: Direct BAG3-HSPB8 binding and ternary-complex assembly with Hsp70 are demonstrated in PMID:27884606. This supports a chaperone-specific refinement of the same partner interaction. HSPB8 variants can alter this association (PMID:28144995). Proposed replacements: protein-folding chaperone binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:A1KXE4-2. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:A1KXE4-2, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:A8MQ03. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:A8MQ03, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:A8MTQ0. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:A8MTQ0, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:O00167-2. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:O00167-2, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:O15496. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:O15496, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:O76083-2. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:O76083-2, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:P14373. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:P14373, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:P31321. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:P31321, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:P50570. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:P50570, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q01196-8. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q01196-8, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q05215. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q05215, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q05BL1. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q05BL1, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q0VD86. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q0VD86, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q13133-3. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q13133-3, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q15038. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q15038, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q15427. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q15427, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q15654. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q15654, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q16082. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q16082, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q16630-2. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q16630-2, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q16825. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q16825, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q6DHY5. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q6DHY5, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q6ZSJ9. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q6ZSJ9, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q7Z783. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q7Z783, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q86TH3. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q86TH3, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q86UU5. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q86UU5, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q86V38. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q86V38, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q86WV8. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q86WV8, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q86XR2. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q86XR2, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q8IUQ4-2. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q8IUQ4-2, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q8N9I9. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q8N9I9, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q8NEA6-2. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q8NEA6-2, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q8WUU8. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q8WUU8, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q8WWB5. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q8WWB5, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q969T9. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q969T9, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q96B67. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q96B67, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q96DV4. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q96DV4, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q96PV6. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q96PV6, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q96RU7. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q96RU7, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q99732. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q99732, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q99750. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q99750, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q9GZU7. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q9GZU7, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q9H1C7. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q9H1C7, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q9H2G9. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q9H2G9, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q9H9H4. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q9H9H4, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q9H9P5-5. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q9H9P5-5, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q9NPF4. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q9NPF4, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q9NQX0. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q9NQX0, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q9NR12. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q9NR12, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q9NU39. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q9NU39, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q9NV39. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q9NV39, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q9UBY9. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q9UBY9, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q9UF11-2. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q9UF11-2, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q9UHR4. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q9UHR4, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: BAG3 binds the protein-folding chaperone HSPB8. Reason: Direct BAG3-HSPB8 binding and ternary-complex assembly with Hsp70 are demonstrated in PMID:27884606. This supports a chaperone-specific refinement of the same partner interaction. HSPB8 variants can alter this association (PMID:28144995). Proposed replacements: protein-folding chaperone binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q9Y5V3. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q9Y5V3, curated from PMID:32296183. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:O76024. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:O76024, curated from PMID:32814053. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:P02489. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:P02489, curated from PMID:32814053. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | MODIFY | Summary: BAG3 binds HSPB1/Hsp27 through its IPV motifs. Reason: GOA identifies the experimental interaction partner as UniProtKB:P04792 (HSPB1/Hsp27). The original pair-specific supplementary result was not independently inspected here. Independent PMID:27884606 directly demonstrates HSPB1 binding, IPV-motif dependence and remodeling of Hsp27 oligomers, supporting the more specific Hsp27-binding term for this same partner. Proposed replacements: Hsp27 protein binding |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:P07196. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:P07196, curated from PMID:32814053. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:P48643. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:P48643, curated from PMID:32814053. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q09028. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q09028, curated from PMID:32814053. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q14204. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q14204, curated from PMID:32814053. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q86V38. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q86V38, curated from PMID:32814053. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q8WZ19. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q8WZ19, curated from PMID:32814053. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q92876. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q92876, curated from PMID:32814053. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q96IK1-2. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q96IK1-2, curated from PMID:32814053. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q9BRX2. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q9BRX2, curated from PMID:32814053. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q9H6L5-2. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q9H6L5-2, curated from PMID:32814053. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q16082. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q16082, curated from PMID:33961781. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | KEEP AS NON CORE | Summary: BAG3 has a curated experimental interaction with UniProtKB:Q9NR12. Reason: GOA records an experimental IPI association of BAG3 with UniProtKB:Q9NR12, curated from PMID:33961781. This reported association is retained as non-core with deference to the experimental curation. The individual supplementary pair result was not independently inspected here, so no more specific molecular activity, direct-substrate relationship or stable-complex membership is inferred for this partner. |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | MODIFY | Summary: BAG3 binds the protein-folding chaperone HSPB8. Reason: Direct BAG3-HSPB8 binding and ternary-complex assembly with Hsp70 are demonstrated in PMID:27884606. This supports a chaperone-specific refinement of the same partner interaction. HSPB8 variants can alter this association (PMID:28144995). Proposed replacements: protein-folding chaperone binding |
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Download this section (compressed HTML)Q: Should GO clarify or separate the CASA scope currently included under GO:0061684 from canonical chaperone-mediated direct lysosomal translocation, given the distinct pathways described in PMID:20060297?
Q: Which BAG3 interactions should be curated as direct molecular functions versus context-specific scaffolding interactions limited to stress, muscle, or disease settings?
Q: How broadly conserved is BAG3-dependent HSF1 nucleocytoplasmic shuttling across human cell types compared with its core cytosolic proteostasis role?
Experiment: Use endogenous BAG3 perturbation and rescue with BAG, IPV, WW, and PxxP mutants in human cardiomyocytes or skeletal myotubes to separate HSP70 NEF, small-HSP scaffold, SYNPO2/filamin, and dynein-loading functions.
Experiment: Quantify endogenous BAG3 localization and interaction partners across basal, heat-stress, proteasome-inhibition, and mechanical-strain conditions using validated antibodies and proximity proteomics.
Experiment: Measure CASA flux for defined clients such as filamin and aggregation-prone reporters after BAG3 knockdown/knockout and rescue, tracking p62/LC3 recruitment, aggresome formation, and lysosomal degradation.
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