id: Q9UL45 gene_symbol: BLOC1S6 product_type: PROTEIN status: COMPLETE taxon: id: NCBITaxon:9606 label: Homo sapiens description: BLOC1S6, also called pallidin, is a small coiled-coil protein in the eight-subunit BLOC-1 complex. It forms a stable assembly with BLOS1 and Cappuccino and links this machinery to membrane-trafficking proteins, including syntaxins. Pallidin occurs in cytosolic and peripheral membrane-associated pools. BLOC-1 supports sorting of endosomal cargo toward melanosomes and other lysosome-related organelles, including platelet dense granules. Human pallidin restores pigmentation and cargo sorting in pallid mouse melanocytes. Pallidin-containing complexes also support selected neuronal cargo-trafficking and neurite-development functions. Biallelic BLOC1S6 defects are associated with Hermansky-Pudlak syndrome type 9; the cellular and clinical manifestations vary with the experimental and patient context. alternative_products: - name: '1' id: Q9UL45-1 - name: '2' id: Q9UL45-2 sequence_note: VSP_009293, VSP_009294 - name: '3' id: Q9UL45-3 sequence_note: Not described existing_annotations: - term: id: GO:0001525 label: angiogenesis evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific angiogenesis assertion requires its unresolved source context. action: UNDECIDED reason: The exact mouse vascular assay was not recovered. Endosomal trafficking alone does not resolve endothelial sprouting or vessel formation. - term: id: GO:0001725 label: stress fiber evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: is_active_in supporting_entities: - MGI:MGI:1927580 - PANTHER:PTN001583105 review: summary: The specific stress fiber assertion requires its unresolved source context. action: UNDECIDED reason: The PAINT placement and its mouse stress-fiber localization experiment remain unresolved. Human overexpression in PMID:12019270 preferentially marks perinuclear actin, with less obvious long-stress-fiber labeling; this context does not contradict an uninspected mouse assay or prove loss of the ancestral function. supported_by: - reference_id: PMID:12019270 - term: id: GO:0001725 label: stress fiber evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: is_active_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific stress fiber assertion requires its unresolved source context. action: UNDECIDED reason: The exact mouse stress-fiber localization experiment remains unresolved. Selected human tagged-protein localization at perinuclear actin cannot substitute for that assay or establish a species-specific absence. supported_by: - reference_id: PMID:12019270 - term: id: GO:0001726 label: ruffle evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: is_active_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific ruffle assertion requires its unresolved source context. action: UNDECIDED reason: Tagged human perinuclear actin association does not establish the source mouse ruffle localization; the specific donor assay remains unverified. supported_by: - reference_id: PMID:12019270 - term: id: GO:0001822 label: kidney development evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific kidney development assertion requires its unresolved source context. action: UNDECIDED reason: Pallid mice have abnormal renal lysosomal-enzyme levels, but that phenotype alone does not establish a kidney-development mechanism. The actual developmental donor experiment remains unverified. - term: id: GO:0001944 label: vasculature development evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific vasculature development assertion requires its unresolved source context. action: UNDECIDED reason: The exact donor vessel-development experiment was not recovered. A general trafficking role cannot settle this developmental assignment. - term: id: GO:0003016 label: respiratory system process evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific respiratory system process assertion requires its unresolved source context. action: UNDECIDED reason: The pallid mouse is a pulmonary-emphysema model, but the precise physiological experiment underlying this respiratory-process annotation remains unverified. - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:12191018 qualifier: enables supporting_entities: - UniProtKB:Q86Y82 review: summary: The source-listed association with STX12 (Q86Y82) can be expressed as syntaxin binding. action: MODIFY reason: Q86Y82 is human syntaxin-12, not a BLOC-1 subunit. The curated IPI pair and the reviewed human UniProt subunit record corroborate this association; the original study is cached only as an abstract and its pair-level experiment was not independently inspected. Refine the existing interaction to the verified partner class. This does not assert a newly mapped isolated pallidin interface or transfer syntaxin catalytic or fusion activity to pallidin. supported_by: - reference_id: PMID:12191018 - reference_id: UniProt:Q9UL45 proposed_replacement_terms: - id: GO:0019905 label: syntaxin binding - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:12191018 qualifier: enables supporting_entities: - UniProtKB:Q8TDH9 review: summary: Pallidin physically associates with UniProtKB:Q8TDH9. action: KEEP_AS_NON_CORE reason: The cached human pallidin abstract establishes a heterooligomer containing Muted; the reviewed UniProt entry also records the source interaction. The exact pair-level experiments were not independently read. Preserve the curated IPI interaction as non-core without claiming an isolated interface from the abstract. supported_by: - reference_id: PMID:12191018 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:15102850 qualifier: enables supporting_entities: - UniProtKB:P78537 review: summary: Pallidin physically associates with UniProtKB:P78537. action: KEEP_AS_NON_CORE reason: The cached abstract describes endogenous BLOC-1 co-immunoprecipitation/co-fractionation and an eight-subunit two-hybrid network. These support the curated physical association. The exact pair matrix was not independently inspected; retain generic binding as non-core rather than reject an experimental assertion from an incomplete cache. supported_by: - reference_id: PMID:15102850 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:15102850 qualifier: enables supporting_entities: - UniProtKB:Q96EV8 review: summary: Pallidin physically associates with UniProtKB:Q96EV8. action: KEEP_AS_NON_CORE reason: The cached abstract describes endogenous BLOC-1 co-immunoprecipitation/co-fractionation and an eight-subunit two-hybrid network. These support the curated physical association. The exact pair matrix was not independently inspected; retain generic binding as non-core rather than reject an experimental assertion from an incomplete cache. supported_by: - reference_id: PMID:15102850 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:15102850 qualifier: enables supporting_entities: - UniProtKB:Q9NUP1 review: summary: Pallidin physically associates with UniProtKB:Q9NUP1. action: KEEP_AS_NON_CORE reason: The cached abstract describes endogenous BLOC-1 co-immunoprecipitation/co-fractionation and an eight-subunit two-hybrid network. These support the curated physical association. The exact pair matrix was not independently inspected; retain generic binding as non-core rather than reject an experimental assertion from an incomplete cache. supported_by: - reference_id: PMID:15102850 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:16189514 qualifier: enables supporting_entities: - UniProtKB:Q8IYI6 review: summary: Pallidin physically associates with UniProtKB:Q8IYI6. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:16189514 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:16189514 qualifier: enables supporting_entities: - UniProtKB:Q8N302 review: summary: Pallidin physically associates with UniProtKB:Q8N302. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:16189514 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:16189514 qualifier: enables supporting_entities: - UniProtKB:Q96BD8 review: summary: Pallidin physically associates with UniProtKB:Q96BD8. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:16189514 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:16189514 qualifier: enables supporting_entities: - UniProtKB:Q9H8W4 review: summary: Pallidin physically associates with UniProtKB:Q9H8W4. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:16189514 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:16189514 qualifier: enables supporting_entities: - UniProtKB:Q9Y3C0 review: summary: Pallidin physically associates with UniProtKB:Q9Y3C0. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:16189514 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:19546860 qualifier: enables supporting_entities: - UniProtKB:A6QP11 review: summary: The source-listed association with bovine SNAP47 (A6QP11) can be expressed as SNARE binding. action: MODIFY reason: Selected PMID:19546860 Methods and Results test native BLOC-1 from bovine brain cytosol by anti-pallidin detection in recombinant SNARE pull-downs. The source-listed partner, bovine SNAP47 (A6QP11), belongs to the SNAP SNARE class. Refine the generic interaction to SNARE binding while preserving the original partner and isoform. The measured association is with the pallidin-containing complex; it does not identify pallidin as the isolated contact surface, confer SNARE receptor activity, or establish a regulatory direction for fusion. supported_by: - reference_id: PMID:19546860 - reference_id: UniProt:Q9UL45 proposed_replacement_terms: - id: GO:0000149 label: SNARE binding - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:19546860 qualifier: enables supporting_entities: - UniProtKB:A7MAZ2 review: summary: The source-listed association with bovine STX12 (A7MAZ2) can be expressed as syntaxin binding. action: MODIFY reason: Selected PMID:19546860 Methods and Results test native BLOC-1 from bovine brain cytosol by anti-pallidin detection in recombinant SNARE pull-downs. The source-listed partner is syntaxin-12/13. The experiments distinguish binding to its open SN fragment from the poorly binding complete cytoplasmic region and distinguish free SNAREs from assembled bundles. Refine this association to syntaxin binding; neither isolated-pallidin contact nor equal recognition of all syntaxins is claimed. The bovine accession-to-STX12 mapping was checked in indexed Bgee gene cross-references; the direct UniProt entry was inaccessible. supported_by: - reference_id: PMID:19546860 - reference_id: UniProt:Q9UL45 proposed_replacement_terms: - id: GO:0019905 label: syntaxin binding - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:19546860 qualifier: enables supporting_entities: - UniProtKB:P60879-1 review: summary: The source-listed association with mouse SNAP25 isoform 1 (P60879-1) can be expressed as SNARE binding. action: MODIFY reason: Selected PMID:19546860 Methods and Results test native BLOC-1 from bovine brain cytosol by anti-pallidin detection in recombinant SNARE pull-downs. The source-listed partner, mouse SNAP25 isoform 1 (P60879-1), belongs to the SNAP SNARE class. Refine the generic interaction to SNARE binding while preserving the original partner and isoform. The measured association is with the pallidin-containing complex; it does not identify pallidin as the isolated contact surface, confer SNARE receptor activity, or establish a regulatory direction for fusion. supported_by: - reference_id: PMID:19546860 - reference_id: UniProt:Q9UL45 proposed_replacement_terms: - id: GO:0000149 label: SNARE binding - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:19546860 qualifier: enables supporting_entities: - UniProtKB:P60880-1 review: summary: The source-listed association with human SNAP25 isoform 1 (P60880-1) can be expressed as SNARE binding. action: MODIFY reason: Selected PMID:19546860 Methods and Results test native BLOC-1 from bovine brain cytosol by anti-pallidin detection in recombinant SNARE pull-downs. The source-listed partner, human SNAP25 isoform 1 (P60880-1), belongs to the SNAP SNARE class. Refine the generic interaction to SNARE binding while preserving the original partner and isoform. The measured association is with the pallidin-containing complex; it does not identify pallidin as the isolated contact surface, confer SNARE receptor activity, or establish a regulatory direction for fusion. supported_by: - reference_id: PMID:19546860 - reference_id: UniProt:Q9UL45 proposed_replacement_terms: - id: GO:0000149 label: SNARE binding - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:19546860 qualifier: enables supporting_entities: - UniProtKB:P60880-2 review: summary: The source-listed association with human SNAP25 isoform 2 (P60880-2) can be expressed as SNARE binding. action: MODIFY reason: Selected PMID:19546860 Methods and Results test native BLOC-1 from bovine brain cytosol by anti-pallidin detection in recombinant SNARE pull-downs. The source-listed partner, human SNAP25 isoform 2 (P60880-2), belongs to the SNAP SNARE class. Refine the generic interaction to SNARE binding while preserving the original partner and isoform. The measured association is with the pallidin-containing complex; it does not identify pallidin as the isolated contact surface, confer SNARE receptor activity, or establish a regulatory direction for fusion. supported_by: - reference_id: PMID:19546860 - reference_id: UniProt:Q9UL45 proposed_replacement_terms: - id: GO:0000149 label: SNARE binding - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:19546860 qualifier: enables supporting_entities: - UniProtKB:Q5SQN1 review: summary: The source-listed association with human SNAP47 (Q5SQN1) can be expressed as SNARE binding. action: MODIFY reason: Selected PMID:19546860 Methods and Results test native BLOC-1 from bovine brain cytosol by anti-pallidin detection in recombinant SNARE pull-downs. The source-listed partner, human SNAP47 (Q5SQN1), belongs to the SNAP SNARE class. Refine the generic interaction to SNARE binding while preserving the original partner and isoform. The measured association is with the pallidin-containing complex; it does not identify pallidin as the isolated contact surface, confer SNARE receptor activity, or establish a regulatory direction for fusion. supported_by: - reference_id: PMID:19546860 - reference_id: UniProt:Q9UL45 proposed_replacement_terms: - id: GO:0000149 label: SNARE binding - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:19546860 qualifier: enables supporting_entities: - UniProtKB:Q86Y82 review: summary: The source-listed association with human STX12 (Q86Y82) can be expressed as syntaxin binding. action: MODIFY reason: Selected PMID:19546860 Methods and Results test native BLOC-1 from bovine brain cytosol by anti-pallidin detection in recombinant SNARE pull-downs. The source-listed partner is syntaxin-12/13. The experiments distinguish binding to its open SN fragment from the poorly binding complete cytoplasmic region and distinguish free SNAREs from assembled bundles. Refine this association to syntaxin binding; neither isolated-pallidin contact nor equal recognition of all syntaxins is claimed. The human Q86Y82-to-STX12 identity was checked in official NCBI and GO gene-product records. supported_by: - reference_id: PMID:19546860 - reference_id: UniProt:Q9UL45 proposed_replacement_terms: - id: GO:0019905 label: syntaxin binding - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:21998198 qualifier: enables supporting_entities: - UniProtKB:Q8R015 review: summary: Pallidin physically associates with UniProtKB:Q8R015. action: KEEP_AS_NON_CORE reason: Selected human SH-SY5Y complex experiments recover endogenous pallidin with tagged BLOC-1 components and cargo-sorting machinery. This substantiates assembly-level physical association, not necessarily a purified binary interface. Retain the curated source association as non-core. supported_by: - reference_id: PMID:21998198 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:22203680 qualifier: enables supporting_entities: - UniProtKB:P78537 review: summary: Pallidin physically associates with UniProtKB:P78537. action: KEEP_AS_NON_CORE reason: The selected human recombinant study directly reconstitutes pallidin-BLOS1 and pallidin-Cappuccino pairs and a stable ternary assembly; dysbindin is in the complete octamer. Retain the original partner-specific association as non-core, distinguishing those isolated interfaces from whole-complex membership. supported_by: - reference_id: PMID:22203680 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:22203680 qualifier: enables supporting_entities: - UniProtKB:Q96EV8 review: summary: Pallidin physically associates with UniProtKB:Q96EV8. action: KEEP_AS_NON_CORE reason: The selected human recombinant study directly reconstitutes pallidin-BLOS1 and pallidin-Cappuccino pairs and a stable ternary assembly; dysbindin is in the complete octamer. Retain the original partner-specific association as non-core, distinguishing those isolated interfaces from whole-complex membership. supported_by: - reference_id: PMID:22203680 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:22203680 qualifier: enables supporting_entities: - UniProtKB:Q9NUP1 review: summary: Pallidin physically associates with UniProtKB:Q9NUP1. action: KEEP_AS_NON_CORE reason: The selected human recombinant study directly reconstitutes pallidin-BLOS1 and pallidin-Cappuccino pairs and a stable ternary assembly; dysbindin is in the complete octamer. Retain the original partner-specific association as non-core, distinguishing those isolated interfaces from whole-complex membership. supported_by: - reference_id: PMID:22203680 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:25416956 qualifier: enables supporting_entities: - UniProtKB:A1A4E9 review: summary: Pallidin physically associates with UniProtKB:A1A4E9. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:25416956 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:25416956 qualifier: enables supporting_entities: - UniProtKB:A1L190 review: summary: Pallidin physically associates with UniProtKB:A1L190. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:25416956 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:25416956 qualifier: enables supporting_entities: - UniProtKB:O75558 review: summary: The source-listed association with STX11 (O75558) can be expressed as syntaxin binding. action: MODIFY reason: The original curated IPI row names O75558, which the reviewed human UniProt record identifies as STX11 and lists as a pallidin interaction. The exact source-specific supplementary pair was not independently inspected. Refine the existing association to syntaxin binding using the verified partner identity, without assigning a physiological vesicle route, isolated binding site, or syntaxin enzymatic activity to pallidin. supported_by: - reference_id: PMID:25416956 - reference_id: UniProt:Q9UL45 proposed_replacement_terms: - id: GO:0019905 label: syntaxin binding - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:25416956 qualifier: enables supporting_entities: - UniProtKB:P37198 review: summary: Pallidin physically associates with UniProtKB:P37198. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:25416956 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:25416956 qualifier: enables supporting_entities: - UniProtKB:P78537 review: summary: Pallidin physically associates with UniProtKB:P78537. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:25416956 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:25416956 qualifier: enables supporting_entities: - UniProtKB:Q13503 review: summary: Pallidin physically associates with UniProtKB:Q13503. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:25416956 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:25416956 qualifier: enables supporting_entities: - UniProtKB:Q13561 review: summary: Pallidin physically associates with UniProtKB:Q13561. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:25416956 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:25416956 qualifier: enables supporting_entities: - UniProtKB:Q16637 review: summary: Pallidin physically associates with UniProtKB:Q16637. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:25416956 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:25416956 qualifier: enables supporting_entities: - UniProtKB:Q5VU62 review: summary: Pallidin physically associates with UniProtKB:Q5VU62. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:25416956 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:25416956 qualifier: enables supporting_entities: - UniProtKB:Q6QEF8 review: summary: Pallidin physically associates with UniProtKB:Q6QEF8. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:25416956 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:25416956 qualifier: enables supporting_entities: - UniProtKB:Q8N619 review: summary: Pallidin physically associates with UniProtKB:Q8N619. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:25416956 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:25416956 qualifier: enables supporting_entities: - UniProtKB:Q8TD31-3 review: summary: Pallidin physically associates with UniProtKB:Q8TD31-3. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:25416956 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:25416956 qualifier: enables supporting_entities: - UniProtKB:Q96BD8 review: summary: Pallidin physically associates with UniProtKB:Q96BD8. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:25416956 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:25416956 qualifier: enables supporting_entities: - UniProtKB:Q96CS2 review: summary: Pallidin physically associates with UniProtKB:Q96CS2. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:25416956 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:25416956 qualifier: enables supporting_entities: - UniProtKB:Q96JN2-2 review: summary: Pallidin physically associates with UniProtKB:Q96JN2-2. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:25416956 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:25416956 qualifier: enables supporting_entities: - UniProtKB:Q9NYB9 review: summary: Pallidin physically associates with UniProtKB:Q9NYB9. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:25416956 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:25416956 qualifier: enables supporting_entities: - UniProtKB:Q9UJ41 review: summary: Pallidin physically associates with UniProtKB:Q9UJ41. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:25416956 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:29892012 qualifier: enables supporting_entities: - UniProtKB:Q9NYB9 review: summary: Pallidin physically associates with UniProtKB:Q9NYB9. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:29892012 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:31515488 qualifier: enables supporting_entities: - UniProtKB:Q96BD8 review: summary: Pallidin physically associates with UniProtKB:Q96BD8. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:31515488 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:31515488 qualifier: enables supporting_entities: - UniProtKB:Q9Y3C0 review: summary: Pallidin physically associates with UniProtKB:Q9Y3C0. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:31515488 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:32296183 qualifier: enables supporting_entities: - UniProtKB:O75558 review: summary: The source-listed association with STX11 (O75558) can be expressed as syntaxin binding. action: MODIFY reason: The original curated IPI row names O75558, which the reviewed human UniProt record identifies as STX11 and lists as a pallidin interaction. The exact source-specific supplementary pair was not independently inspected. Refine the existing association to syntaxin binding using the verified partner identity, without assigning a physiological vesicle route, isolated binding site, or syntaxin enzymatic activity to pallidin. supported_by: - reference_id: PMID:32296183 - reference_id: UniProt:Q9UL45 proposed_replacement_terms: - id: GO:0019905 label: syntaxin binding - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:32296183 qualifier: enables supporting_entities: - UniProtKB:P09493-10 review: summary: Pallidin physically associates with UniProtKB:P09493-10. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:32296183 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:32296183 qualifier: enables supporting_entities: - UniProtKB:P37198 review: summary: Pallidin physically associates with UniProtKB:P37198. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:32296183 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:32296183 qualifier: enables supporting_entities: - UniProtKB:P78537 review: summary: Pallidin physically associates with UniProtKB:P78537. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:32296183 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:32296183 qualifier: enables supporting_entities: - UniProtKB:Q14CN4 review: summary: Pallidin physically associates with UniProtKB:Q14CN4. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:32296183 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:32296183 qualifier: enables supporting_entities: - UniProtKB:Q16623 review: summary: The source-listed association with STX1A (Q16623) can be expressed as syntaxin-1 binding. action: MODIFY reason: The original curated IPI pair names Q16623, identified as human STX1A in the reviewed UniProt interaction record. The official GO definition of syntaxin-1 binding matches this partner more precisely than generic protein binding. The exact supplementary pair-level experiment was not independently inspected; retain that limit and do not infer a native synaptic mechanism merely from this interaction. supported_by: - reference_id: PMID:32296183 - reference_id: UniProt:Q9UL45 proposed_replacement_terms: - id: GO:0017075 label: syntaxin-1 binding - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:32296183 qualifier: enables supporting_entities: - UniProtKB:Q16637 review: summary: Pallidin physically associates with UniProtKB:Q16637. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:32296183 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:32296183 qualifier: enables supporting_entities: - UniProtKB:Q2T9L4 review: summary: Pallidin physically associates with UniProtKB:Q2T9L4. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:32296183 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:32296183 qualifier: enables supporting_entities: - UniProtKB:Q504U0 review: summary: Pallidin physically associates with UniProtKB:Q504U0. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:32296183 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:32296183 qualifier: enables supporting_entities: - UniProtKB:Q6NUQ1 review: summary: Pallidin physically associates with UniProtKB:Q6NUQ1. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:32296183 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:32296183 qualifier: enables supporting_entities: - UniProtKB:Q8IY31-3 review: summary: Pallidin physically associates with UniProtKB:Q8IY31-3. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:32296183 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:32296183 qualifier: enables supporting_entities: - UniProtKB:Q8IYI6 review: summary: Pallidin physically associates with UniProtKB:Q8IYI6. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:32296183 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:32296183 qualifier: enables supporting_entities: - UniProtKB:Q96BD8 review: summary: Pallidin physically associates with UniProtKB:Q96BD8. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:32296183 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:32296183 qualifier: enables supporting_entities: - UniProtKB:Q96EV8 review: summary: Pallidin physically associates with UniProtKB:Q96EV8. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:32296183 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:32296183 qualifier: enables supporting_entities: - UniProtKB:Q9BV36 review: summary: Pallidin physically associates with UniProtKB:Q9BV36. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:32296183 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:32296183 qualifier: enables supporting_entities: - UniProtKB:Q9C0F1 review: summary: Pallidin physically associates with UniProtKB:Q9C0F1. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:32296183 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:32296183 qualifier: enables supporting_entities: - UniProtKB:Q9H8W4 review: summary: Pallidin physically associates with UniProtKB:Q9H8W4. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:32296183 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:32296183 qualifier: enables supporting_entities: - UniProtKB:Q9NYB9-2 review: summary: Pallidin physically associates with UniProtKB:Q9NYB9-2. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:32296183 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:32296183 qualifier: enables supporting_entities: - UniProtKB:Q9UJ41-4 review: summary: Pallidin physically associates with UniProtKB:Q9UJ41-4. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:32296183 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:32296183 qualifier: enables supporting_entities: - UniProtKB:Q9Y3C0 review: summary: Pallidin physically associates with UniProtKB:Q9Y3C0. action: KEEP_AS_NON_CORE reason: The cached abstract establishes a systematic human interaction or variant-perturbation study. The exact pair-level supplementary experiment was not independently inspected. Preserve this curated experimental interaction as non-core, with its original partner identifier, rather than infer the partner's biological function or reject it from abstract-only evidence. supported_by: - reference_id: PMID:32296183 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:33961781 qualifier: enables supporting_entities: - UniProtKB:P78537 review: summary: Pallidin physically associates with UniProtKB:P78537. action: KEEP_AS_NON_CORE reason: BioPlex affinity-purification mass spectrometry maps physical associations in human cell lines. The source record is retained as non-core; the pair-level supplementary dataset was not independently inspected, and co-purification does not by itself establish a direct binary interface. supported_by: - reference_id: PMID:33961781 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:33961781 qualifier: enables supporting_entities: - UniProtKB:Q8N302 review: summary: Pallidin physically associates with UniProtKB:Q8N302. action: KEEP_AS_NON_CORE reason: BioPlex affinity-purification mass spectrometry maps physical associations in human cell lines. The source record is retained as non-core; the pair-level supplementary dataset was not independently inspected, and co-purification does not by itself establish a direct binary interface. supported_by: - reference_id: PMID:33961781 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:33961781 qualifier: enables supporting_entities: - UniProtKB:Q96BD8 review: summary: Pallidin physically associates with UniProtKB:Q96BD8. action: KEEP_AS_NON_CORE reason: BioPlex affinity-purification mass spectrometry maps physical associations in human cell lines. The source record is retained as non-core; the pair-level supplementary dataset was not independently inspected, and co-purification does not by itself establish a direct binary interface. supported_by: - reference_id: PMID:33961781 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:33961781 qualifier: enables supporting_entities: - UniProtKB:Q96EV8 review: summary: Pallidin physically associates with UniProtKB:Q96EV8. action: KEEP_AS_NON_CORE reason: BioPlex affinity-purification mass spectrometry maps physical associations in human cell lines. The source record is retained as non-core; the pair-level supplementary dataset was not independently inspected, and co-purification does not by itself establish a direct binary interface. supported_by: - reference_id: PMID:33961781 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:33961781 qualifier: enables supporting_entities: - UniProtKB:Q9C0F1 review: summary: Pallidin physically associates with UniProtKB:Q9C0F1. action: KEEP_AS_NON_CORE reason: BioPlex affinity-purification mass spectrometry maps physical associations in human cell lines. The source record is retained as non-core; the pair-level supplementary dataset was not independently inspected, and co-purification does not by itself establish a direct binary interface. supported_by: - reference_id: PMID:33961781 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:33961781 qualifier: enables supporting_entities: - UniProtKB:Q9NUP1 review: summary: Pallidin physically associates with UniProtKB:Q9NUP1. action: KEEP_AS_NON_CORE reason: BioPlex affinity-purification mass spectrometry maps physical associations in human cell lines. The source record is retained as non-core; the pair-level supplementary dataset was not independently inspected, and co-purification does not by itself establish a direct binary interface. supported_by: - reference_id: PMID:33961781 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:33961781 qualifier: enables supporting_entities: - UniProtKB:Q9Y3C0 review: summary: Pallidin physically associates with UniProtKB:Q9Y3C0. action: KEEP_AS_NON_CORE reason: BioPlex affinity-purification mass spectrometry maps physical associations in human cell lines. The source record is retained as non-core; the pair-level supplementary dataset was not independently inspected, and co-purification does not by itself establish a direct binary interface. supported_by: - reference_id: PMID:33961781 - reference_id: UniProt:Q9UL45 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:40205054 qualifier: enables supporting_entities: - UniProtKB:Q96EV8 review: summary: Pallidin physically associates with UniProtKB:Q96EV8. action: KEEP_AS_NON_CORE reason: The cached abstract establishes the multimodal human cell-map context. The curated pallidin-dysbindin association agrees with BLOC-1 membership; the pair-level data were not independently reanalyzed. The official correction changes a duplicated loss-function equation and does not identify a change to this interaction. Retain the physical association as non-core. supported_by: - reference_id: PMID:40205054 - reference_id: UniProt:Q9UL45 - term: id: GO:0005737 label: cytoplasm evidence_type: IDA original_reference_id: PMID:12019270 qualifier: located_in review: summary: Pallidin has a cytoplasmic pool. action: ACCEPT reason: Human pallidin biochemical fractionation and the reviewed UniProt record establish a soluble cytoplasmic pool together with peripheral membrane association. This broad location is consistent with the BLOC-1 trafficking role. supported_by: - reference_id: PMID:12019270 supporting_text: "Endogenous pallidin was distributed in both soluble and\nperipheral membrane protein fractions." - reference_id: PMID:12191018 - reference_id: UniProt:Q9UL45 - term: id: GO:0005737 label: cytoplasm evidence_type: IDA original_reference_id: PMID:12191018 qualifier: located_in review: summary: Pallidin has a cytoplasmic pool. action: ACCEPT reason: Human pallidin biochemical fractionation and the reviewed UniProt record establish a soluble cytoplasmic pool together with peripheral membrane association. This broad location is consistent with the BLOC-1 trafficking role. supported_by: - reference_id: PMID:12019270 - reference_id: PMID:12191018 - reference_id: UniProt:Q9UL45 - term: id: GO:0005737 label: cytoplasm evidence_type: IEA original_reference_id: GO_REF:0000044 qualifier: located_in supporting_entities: - UniProtKB-SubCell:SL-0086 review: summary: Pallidin has a cytoplasmic pool. action: ACCEPT reason: Human pallidin biochemical fractionation and the reviewed UniProt record establish a soluble cytoplasmic pool together with peripheral membrane association. This broad location is consistent with the BLOC-1 trafficking role. supported_by: - reference_id: PMID:12019270 - reference_id: PMID:12191018 - reference_id: UniProt:Q9UL45 - term: id: GO:0005768 label: endosome evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: located_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: Pallidin-containing BLOC-1 acts at endosomal membranes. action: ACCEPT reason: Human PaHA complementation in pallid mouse melanocytes restores Tyrp1 sorting from early endosomes toward melanosomes; selected endogenous-complex work independently supports membrane association. Retain the mouse-derived endosomal assignment without implying an intraluminal topology. supported_by: - reference_id: PMID:17182842 - reference_id: PMID:12019270 - term: id: GO:0005788 label: endoplasmic reticulum lumen evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: is_active_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: ER-lumen localization overstates the established topology of pallidin. action: MARK_AS_OVER_ANNOTATED reason: The ER lumen is separated from cytosolic BLOC-1 by the ER membrane. Human pallidin is characterized as a cytoplasmic, peripheral-membrane coiled-coil subunit, with no annotated signal peptide or membrane-spanning segment. A lumen assignment therefore extends beyond the supported compartment and topology. Mark this electronic transfer as over-annotated without asserting that its unresolved mouse source is experimentally false or that all unconventional import routes are impossible. The established cytoplasmic and membrane-associated assertions are retained; no replacement location is invented. - term: id: GO:0005829 label: cytosol evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: is_active_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: Pallidin is a cytosolic BLOC-1 subunit. action: ACCEPT reason: The cytosolic biochemical pool is directly supported by human pallidin studies. Reactome R-HSA-429815 describes formation of the eight-component BLOC-1 assembly; the independent fractionation evidence supplies the localization support rather than relying on an event title alone. supported_by: - reference_id: PMID:12019270 - reference_id: PMID:12191018 - reference_id: Reactome:R-HSA-429815 - term: id: GO:0005829 label: cytosol evidence_type: TAS original_reference_id: Reactome:R-HSA-429815 qualifier: located_in review: summary: Pallidin is a cytosolic BLOC-1 subunit. action: ACCEPT reason: The cytosolic biochemical pool is directly supported by human pallidin studies. Reactome R-HSA-429815 describes formation of the eight-component BLOC-1 assembly; the independent fractionation evidence supplies the localization support rather than relying on an event title alone. supported_by: - reference_id: PMID:12019270 - reference_id: PMID:12191018 - reference_id: Reactome:R-HSA-429815 - term: id: GO:0005884 label: actin filament evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: is_active_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: Pallidin-containing material associates with filamentous actin. action: KEEP_AS_NON_CORE reason: Selected PMID:12019270 methods and results show pallidin-containing cytosolic material sedimenting with purified F-actin and tagged human pallidin at perinuclear actin structures. The authors explicitly leave direct binding versus mediation by other actin-associated proteins unresolved. Preserve the experimental and transferred association as non-core without assigning pallidin an actin-polymerizing or crosslinking mechanism; the filamentous-actin part_of assertion is read as association, not incorporation as an actin monomer. supported_by: - reference_id: PMID:12019270 - term: id: GO:0005925 label: focal adhesion evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: is_active_in supporting_entities: - MGI:MGI:1927580 - PANTHER:PTN001583105 review: summary: The specific focal adhesion assertion requires its unresolved source context. action: UNDECIDED reason: The exact mouse focal-adhesion experiment and the PAINT node placement were not reconstructed. BLOC-1 membership and F-actin association do not by themselves establish this localization; no donor-count or name-confusion argument is used. - term: id: GO:0005925 label: focal adhesion evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: is_active_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific focal adhesion assertion requires its unresolved source context. action: UNDECIDED reason: The actual mouse focal-adhesion localization assay remains unverified. Perinuclear F-actin association is a different localization and neither confirms nor refutes the donor experiment. - term: id: GO:0006520 label: amino acid metabolic process evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: Altered metabolite abundance does not establish direct participation of pallidin in amino acid metabolic process. action: MARK_AS_OVER_ANNOTATED reason: PMID:28701731 compares postnatal day 1–2 pallid and control mouse hippocampi by untargeted metabolomics and reports altered metabolite pools and increased SNAT1 abundance. The selected Results, Table 1, Discussion and Methods support a downstream metabolic consequence of BLOC-1 deficiency; they do not demonstrate pallidin performing a reaction or supplying a metabolic scaffold or cofactor. This distinction concerns participation in the named metabolic process, not merely absence of catalytic activity. The exact current mouse donor GO-row/reference edge remains unresolved, so this study is independent corroboration rather than a claimed reconstruction of that transfer. supported_by: - reference_id: PMID:28701731 - term: id: GO:0006531 label: aspartate metabolic process evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: Altered metabolite abundance does not establish direct participation of pallidin in aspartate metabolic process. action: MARK_AS_OVER_ANNOTATED reason: PMID:28701731 compares postnatal day 1–2 pallid and control mouse hippocampi by untargeted metabolomics and reports altered metabolite pools and increased SNAT1 abundance. The selected Results, Table 1, Discussion and Methods support a downstream metabolic consequence of BLOC-1 deficiency; they do not demonstrate pallidin performing a reaction or supplying a metabolic scaffold or cofactor. This distinction concerns participation in the named metabolic process, not merely absence of catalytic activity. The exact current mouse donor GO-row/reference edge remains unresolved, so this study is independent corroboration rather than a claimed reconstruction of that transfer. supported_by: - reference_id: PMID:28701731 - term: id: GO:0006536 label: glutamate metabolic process evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: Altered metabolite abundance does not establish direct participation of pallidin in glutamate metabolic process. action: MARK_AS_OVER_ANNOTATED reason: PMID:28701731 compares postnatal day 1–2 pallid and control mouse hippocampi by untargeted metabolomics and reports altered metabolite pools and increased SNAT1 abundance. The selected Results, Table 1, Discussion and Methods support a downstream metabolic consequence of BLOC-1 deficiency; they do not demonstrate pallidin performing a reaction or supplying a metabolic scaffold or cofactor. This distinction concerns participation in the named metabolic process, not merely absence of catalytic activity. The exact current mouse donor GO-row/reference edge remains unresolved, so this study is independent corroboration rather than a claimed reconstruction of that transfer. supported_by: - reference_id: PMID:28701731 - term: id: GO:0006541 label: L-glutamine metabolic process evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: Altered metabolite abundance does not establish direct participation of pallidin in L-glutamine metabolic process. action: MARK_AS_OVER_ANNOTATED reason: PMID:28701731 compares postnatal day 1–2 pallid and control mouse hippocampi by untargeted metabolomics and reports altered metabolite pools and increased SNAT1 abundance. The selected Results, Table 1, Discussion and Methods support a downstream metabolic consequence of BLOC-1 deficiency; they do not demonstrate pallidin performing a reaction or supplying a metabolic scaffold or cofactor. This distinction concerns participation in the named metabolic process, not merely absence of catalytic activity. The exact current mouse donor GO-row/reference edge remains unresolved, so this study is independent corroboration rather than a claimed reconstruction of that transfer. supported_by: - reference_id: PMID:28701731 - term: id: GO:0006644 label: phospholipid metabolic process evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: Altered metabolite abundance does not establish direct participation of pallidin in phospholipid metabolic process. action: MARK_AS_OVER_ANNOTATED reason: PMID:28701731 compares postnatal day 1–2 pallid and control mouse hippocampi by untargeted metabolomics and reports altered metabolite pools and increased SNAT1 abundance. The selected Results, Table 1, Discussion and Methods support a downstream metabolic consequence of BLOC-1 deficiency; they do not demonstrate pallidin performing a reaction or supplying a metabolic scaffold or cofactor. This distinction concerns participation in the named metabolic process, not merely absence of catalytic activity. The exact current mouse donor GO-row/reference edge remains unresolved, so this study is independent corroboration rather than a claimed reconstruction of that transfer. supported_by: - reference_id: PMID:28701731 - term: id: GO:0006954 label: inflammatory response evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: Pallidin deficiency affects inflammatory neutrophil function and host defense in mice. action: KEEP_AS_NON_CORE reason: Independently inspected primary PMID:17785837 experiments show impaired elastase release by pallid mouse inflammatory neutrophils, reduced support of parasite killing by infected macrophages, and impaired parasite control after neutrophil transfer. These support a non-core physiological consequence of BLOC-1-dependent granule release. Pallidin is not the assayed protease, immune receptor or oxidant-generating enzyme. The current mouse GO-row/reference edge remains unresolved; this study is independent corroboration, not a claimed reconstruction of the transfer. supported_by: - reference_id: PMID:17785837 - term: id: GO:0006955 label: immune response evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: Pallidin deficiency affects inflammatory neutrophil function and host defense in mice. action: KEEP_AS_NON_CORE reason: Independently inspected primary PMID:17785837 experiments show impaired elastase release by pallid mouse inflammatory neutrophils, reduced support of parasite killing by infected macrophages, and impaired parasite control after neutrophil transfer. These support a non-core physiological consequence of BLOC-1-dependent granule release. Pallidin is not the assayed protease, immune receptor or oxidant-generating enzyme. The current mouse GO-row/reference edge remains unresolved; this study is independent corroboration, not a claimed reconstruction of the transfer. supported_by: - reference_id: PMID:17785837 - term: id: GO:0006979 label: response to oxidative stress evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific response to oxidative stress assertion requires its unresolved source context. action: UNDECIDED reason: 'Positive redox evidence exists: selected primary PMID:22423091 results and Figure 7 caption describe increased basal oxidant signal and reduced peroxiredoxins after pallidin shRNA in human SH-SY5Y cells. Basal oxidant accumulation following protein loss does not alone establish the specific response-to-oxidative-stress process or pallidin peroxidase chemistry. The exact mouse donor-source edge and term fit remain unresolved, so retain UNDECIDED without denying that phenotype.' supported_by: - reference_id: PMID:22423091 - term: id: GO:0007010 label: cytoskeleton organization evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific cytoskeleton organization assertion requires its unresolved source context. action: UNDECIDED reason: Association with F-actin and the primary neurite phenotype do not isolate a pallidin-dependent cytoskeletal-organization step. The source mouse organization assay remains unverified. supported_by: - reference_id: PMID:12019270 - term: id: GO:0007032 label: endosome organization evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: BLOC-1 supports endosomal organization and cargo sorting. action: ACCEPT reason: Pallid melanocytes show altered Tyrp1 routing and endosomal cargo distribution, with restoration by human PaHA. This establishes a contribution of the pallidin-containing assembly to the endosomal pathway. The 2017 correction revises the transferrin control to a mild early recycling defect; it does not erase the independently supported Tyrp1 result. supported_by: - reference_id: PMID:17182842 - reference_id: PMID:22203680 - term: id: GO:0007613 label: memory evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific memory assertion requires its unresolved source context. action: UNDECIDED reason: The inspected neonatal hippocampal and neurite studies do not establish the exact adult learning or memory assay used by the donor annotation. - term: id: GO:0008021 label: synaptic vesicle evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: is_active_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific synaptic vesicle assertion requires its unresolved source context. action: UNDECIDED reason: The inspected neuronal experiments concern BLOC-1-associated cargo and small intracellular vesicle fractions. They do not independently resolve pallidin itself on mature synaptic vesicles; the exact donor localization remains unverified. - term: id: GO:0008089 label: anterograde axonal transport evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: BLOC-1 supports delivery of selected neuronal membrane cargo. action: KEEP_AS_NON_CORE reason: Selected PMID:21998198 experiments include pallid mouse fibroblasts and dentate-gyrus cargo distribution, together with human neuronal-cell complexes containing endogenous pallidin. These support the neuronal trafficking pathway and the curator-mediated orthology inferences, rather than pallidin acting as an axonal motor or transporting every mature synaptic vesicle. Keep this neuronal specialization as non-core. supported_by: - reference_id: PMID:21998198 - reference_id: UniProt:Q9UL45 - term: id: GO:0008089 label: anterograde axonal transport evidence_type: ISS original_reference_id: GO_REF:0000024 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 review: summary: BLOC-1 supports delivery of selected neuronal membrane cargo. action: KEEP_AS_NON_CORE reason: Selected PMID:21998198 experiments include pallid mouse fibroblasts and dentate-gyrus cargo distribution, together with human neuronal-cell complexes containing endogenous pallidin. These support the neuronal trafficking pathway and the curator-mediated orthology inferences, rather than pallidin acting as an axonal motor or transporting every mature synaptic vesicle. Keep this neuronal specialization as non-core. supported_by: - reference_id: PMID:21998198 - reference_id: UniProt:Q9UL45 - term: id: GO:0009306 label: protein secretion evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: Pallidin supports granule-protein release in a trafficking-dependent physiological context. action: KEEP_AS_NON_CORE reason: PMID:17785837 directly measures reduced elastase release from living pallid mouse inflammatory neutrophils. Human patient NK-cell experiments in the independently identified 2012 PMID:22461475 also show impaired degranulation, abnormal lysosomal-marker distribution and correction of CD107a distribution by PLDN replacement. Together with pallidin’s role in BLOC-1 trafficking machinery, these observations support retaining this existing secretion annotation as non-core. Pallidin contributes trafficking machinery; it is neither the secreted elastase substrate nor its protease. The specific molecular step in neutrophil release and the exact current donor GO-row/reference edge remain unresolved. supported_by: - reference_id: PMID:17785837 - reference_id: PMID:22461475 - term: id: GO:0010467 label: gene expression evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The functional basis of the transferred gene-expression assertion remains unresolved. action: UNDECIDED reason: The current mouse experiment underlying this electronic transfer has not been identified. Pallidin has an established BLOC-1 trafficking role, but that fact alone cannot exclude every additional activity in RNA or protein expression. Changed downstream abundance would not by itself demonstrate participation; it has not been established that such a measurement was the sole basis of this donor annotation. Retain UNDECIDED for the missing source context instead of treating a hypothetical experiment as an inspected refutation. - term: id: GO:0014823 label: response to activity evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific response to activity assertion requires its unresolved source context. action: UNDECIDED reason: The precise activity-response experiment in the mouse donor is unresolved. The fly high-activity recycling result is useful context but cannot reconstruct this particular transfer. - term: id: GO:0016020 label: membrane evidence_type: EXP original_reference_id: PMID:12019270 qualifier: located_in review: summary: Pallidin associates peripherally with membranes. action: ACCEPT reason: Human pallidin occurs in soluble and membrane-associated fractions, and the inspected studies characterize its membrane pool as peripheral. Retain the broad membrane location without turning this non-transmembrane BLOC-1 subunit into a membrane-spanning transporter. supported_by: - reference_id: PMID:12019270 - reference_id: PMID:12191018 - reference_id: UniProt:Q9UL45 - term: id: GO:0016020 label: membrane evidence_type: EXP original_reference_id: PMID:12191018 qualifier: located_in review: summary: Pallidin associates peripherally with membranes. action: ACCEPT reason: Human pallidin occurs in soluble and membrane-associated fractions, and the inspected studies characterize its membrane pool as peripheral. Retain the broad membrane location without turning this non-transmembrane BLOC-1 subunit into a membrane-spanning transporter. supported_by: - reference_id: PMID:12019270 - reference_id: PMID:12191018 - reference_id: UniProt:Q9UL45 - term: id: GO:0016020 label: membrane evidence_type: IEA original_reference_id: GO_REF:0000120 qualifier: located_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 - UniProtKB-SubCell:SL-0162 review: summary: Pallidin associates peripherally with membranes. action: ACCEPT reason: Human pallidin occurs in soluble and membrane-associated fractions, and the inspected studies characterize its membrane pool as peripheral. Retain the broad membrane location without turning this non-transmembrane BLOC-1 subunit into a membrane-spanning transporter. supported_by: - reference_id: PMID:12019270 - reference_id: PMID:12191018 - reference_id: UniProt:Q9UL45 - term: id: GO:0016197 label: endosomal transport evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: BLOC-1 supports endosomal organization and cargo sorting. action: ACCEPT reason: Pallid melanocytes show altered Tyrp1 routing and endosomal cargo distribution, with restoration by human PaHA. This establishes a contribution of the pallidin-containing assembly to the endosomal pathway. The 2017 correction revises the transferrin control to a mild early recycling defect; it does not erase the independently supported Tyrp1 result. supported_by: - reference_id: PMID:17182842 - reference_id: PMID:22203680 - term: id: GO:0019905 label: syntaxin binding evidence_type: TAS original_reference_id: PMID:10610180 qualifier: enables review: summary: Pallidin associates with syntaxin trafficking machinery. action: ACCEPT reason: The complete normal abstract of PMID:10610180 reports a syntaxin-13 interaction identified by two-hybrid analysis and confirmed by co-immunoprecipitation. This is the original curated TAS source, alongside the human UniProt interaction record. Later native brain BLOC-1 experiments distinguish open syntaxin binding from closed or assembled SNARE states; they do not imply binding to every syntaxin. supported_by: - reference_id: PMID:10610180 supporting_text: "pallidin\ninteracts with syntaxin 13" - reference_id: PMID:19546860 - reference_id: UniProt:Q9UL45 - term: id: GO:0021542 label: dentate gyrus development evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific dentate gyrus development assertion requires its unresolved source context. action: UNDECIDED reason: Dentate-gyrus cargo distribution is measured in pallid mice, but that is not itself a developmental assay of formation of the dentate gyrus. The exact developmental evidence remains unverified. - term: id: GO:0021854 label: hypothalamus development evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific hypothalamus development assertion requires its unresolved source context. action: UNDECIDED reason: The selected neuronal trafficking evidence does not resolve hypothalamus development; its specific mouse donor experiment remains unverified. - term: id: GO:0030133 label: transport vesicle evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: is_active_in supporting_entities: - MGI:MGI:1927580 - PANTHER:PTN001583105 - UniProtKB:Q9UL45 review: summary: The specific transport vesicle assertion requires its unresolved source context. action: UNDECIDED reason: The target self-entry in the IBA is legitimate experimental grounding, not circularity. The inspected localization passage in PMID:17182842 resolves MuHA-reconstituted BLOC-1 on tubulovesicular endosomes; it does not separately map pallidin there. The exact pallidin carrier-localization basis, including uninspected supplementary material and PAINT placement, remains unresolved. supported_by: - reference_id: PMID:17182842 - term: id: GO:0030133 label: transport vesicle evidence_type: IDA original_reference_id: PMID:17182842 qualifier: located_in review: summary: The specific transport vesicle assertion requires its unresolved source context. action: UNDECIDED reason: Selected PMID:17182842 body text establishes human PaHA rescue and separately maps MuHA-reconstituted BLOC-1 on tubulovesicular endosomes. The exact pallidin-specific localization assay or supplementary image underlying this IDA was not independently resolved; preserve the experimental assertion as undecided instead of rejecting it from incomplete coverage. supported_by: - reference_id: PMID:17182842 - term: id: GO:0030324 label: lung development evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific lung development assertion requires its unresolved source context. action: UNDECIDED reason: The exact mouse lung-development assay remains unverified. Pulmonary pathology does not by itself identify a developmental rather than maintenance defect. - term: id: GO:0031083 label: BLOC-1 complex evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: part_of supporting_entities: - FB:FBgn0036192 - MGI:MGI:1927580 - PANTHER:PTN001583105 - UniProtKB:Q9UL45 review: summary: Pallidin is an integral BLOC-1 complex subunit. action: ACCEPT reason: Human biochemical studies and complete recombinant octamer reconstitution establish pallidin membership. Pallidin, Cappuccino and BLOS1 form a stable subassembly. The IBA target self-entry represents legitimate descendant experimental grounding, not circular evidence; the full PAINT ancestral placement was not independently reconstructed. supported_by: - reference_id: PMID:12019270 - reference_id: PMID:15102850 - reference_id: PMID:22203680 - reference_id: UniProt:Q9UL45 - term: id: GO:0031083 label: BLOC-1 complex evidence_type: IDA original_reference_id: PMID:12019270 qualifier: part_of review: summary: Pallidin is an integral BLOC-1 complex subunit. action: ACCEPT reason: Human biochemical studies and complete recombinant octamer reconstitution establish pallidin membership. Pallidin, Cappuccino and BLOS1 form a stable subassembly. The IBA target self-entry represents legitimate descendant experimental grounding, not circular evidence; the full PAINT ancestral placement was not independently reconstructed. supported_by: - reference_id: PMID:12019270 - reference_id: PMID:15102850 - reference_id: PMID:22203680 - reference_id: UniProt:Q9UL45 - term: id: GO:0031083 label: BLOC-1 complex evidence_type: IDA original_reference_id: PMID:15102850 qualifier: part_of review: summary: Pallidin is an integral BLOC-1 complex subunit. action: ACCEPT reason: Human biochemical studies and complete recombinant octamer reconstitution establish pallidin membership. Pallidin, Cappuccino and BLOS1 form a stable subassembly. The IBA target self-entry represents legitimate descendant experimental grounding, not circular evidence; the full PAINT ancestral placement was not independently reconstructed. supported_by: - reference_id: PMID:12019270 - reference_id: PMID:15102850 - reference_id: PMID:22203680 - reference_id: UniProt:Q9UL45 - term: id: GO:0031083 label: BLOC-1 complex evidence_type: IDA original_reference_id: PMID:17182842 qualifier: part_of review: summary: Pallidin is an integral BLOC-1 complex subunit. action: ACCEPT reason: Human biochemical studies and complete recombinant octamer reconstitution establish pallidin membership. Pallidin, Cappuccino and BLOS1 form a stable subassembly. The IBA target self-entry represents legitimate descendant experimental grounding, not circular evidence; the full PAINT ancestral placement was not independently reconstructed. supported_by: - reference_id: PMID:12019270 - reference_id: PMID:15102850 - reference_id: PMID:22203680 - reference_id: UniProt:Q9UL45 - term: id: GO:0031083 label: BLOC-1 complex evidence_type: IDA original_reference_id: PMID:22203680 qualifier: part_of review: summary: Pallidin is an integral BLOC-1 complex subunit. action: ACCEPT reason: Human biochemical studies and complete recombinant octamer reconstitution establish pallidin membership. Pallidin, Cappuccino and BLOS1 form a stable subassembly. The IBA target self-entry represents legitimate descendant experimental grounding, not circular evidence; the full PAINT ancestral placement was not independently reconstructed. supported_by: - reference_id: PMID:12019270 - reference_id: PMID:15102850 - reference_id: PMID:22203680 - reference_id: UniProt:Q9UL45 - term: id: GO:0031083 label: BLOC-1 complex evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: part_of supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: Pallidin is an integral BLOC-1 complex subunit. action: ACCEPT reason: Human biochemical studies and complete recombinant octamer reconstitution establish pallidin membership. Pallidin, Cappuccino and BLOS1 form a stable subassembly. The IBA target self-entry represents legitimate descendant experimental grounding, not circular evidence; the full PAINT ancestral placement was not independently reconstructed. supported_by: - reference_id: PMID:12019270 - reference_id: PMID:15102850 - reference_id: PMID:22203680 - reference_id: UniProt:Q9UL45 - term: id: GO:0031083 label: BLOC-1 complex evidence_type: IPI original_reference_id: PMID:22203680 qualifier: part_of review: summary: Pallidin is an integral BLOC-1 complex subunit. action: ACCEPT reason: Human biochemical studies and complete recombinant octamer reconstitution establish pallidin membership. Pallidin, Cappuccino and BLOS1 form a stable subassembly. The IBA target self-entry represents legitimate descendant experimental grounding, not circular evidence; the full PAINT ancestral placement was not independently reconstructed. supported_by: - reference_id: PMID:12019270 - reference_id: PMID:15102850 - reference_id: PMID:22203680 - reference_id: UniProt:Q9UL45 - term: id: GO:0031175 label: neuron projection development evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: Pallidin deficiency affects neurite development. action: KEEP_AS_NON_CORE reason: PMID:19546860 directly compares primary pallid and control mouse hippocampal neurons and finds modest reductions in neurite number and total length. This is target-relevant experimental evidence despite the title foregrounding dysbindin. Retain the human orthology-based developmental annotation as non-core, without claiming a separately demonstrated human neuron mechanism. supported_by: - reference_id: PMID:19546860 - term: id: GO:0031175 label: neuron projection development evidence_type: ISS original_reference_id: GO_REF:0000024 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 review: summary: Pallidin deficiency affects neurite development. action: KEEP_AS_NON_CORE reason: PMID:19546860 directly compares primary pallid and control mouse hippocampal neurons and finds modest reductions in neurite number and total length. This is target-relevant experimental evidence despite the title foregrounding dysbindin. Retain the human orthology-based developmental annotation as non-core, without claiming a separately demonstrated human neuron mechanism. supported_by: - reference_id: PMID:19546860 - term: id: GO:0031410 label: cytoplasmic vesicle evidence_type: IEA original_reference_id: GO_REF:0000117 qualifier: located_in supporting_entities: - ARBA:ARBA00027190 review: summary: BLOC-1 associates with cytoplasmic vesicular trafficking compartments. action: KEEP_AS_NON_CORE reason: The ARBA assignment is broadly consistent with the peripheral membrane pool and endosomal trafficking function of pallidin-containing BLOC-1. Retain this broad inferred location as non-core; it is not a separately resolved pallidin localization experiment for every vesicle class. supported_by: - reference_id: PMID:12019270 - reference_id: PMID:17182842 - term: id: GO:0031941 label: filamentous actin evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: part_of supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: Pallidin-containing material associates with filamentous actin. action: KEEP_AS_NON_CORE reason: Selected PMID:12019270 methods and results show pallidin-containing cytosolic material sedimenting with purified F-actin and tagged human pallidin at perinuclear actin structures. The authors explicitly leave direct binding versus mediation by other actin-associated proteins unresolved. Preserve the experimental and transferred association as non-core without assigning pallidin an actin-polymerizing or crosslinking mechanism; the filamentous-actin part_of assertion is read as association, not incorporation as an actin monomer. supported_by: - reference_id: PMID:12019270 - term: id: GO:0032400 label: melanosome localization evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific melanosome localization assertion requires its unresolved source context. action: UNDECIDED reason: The inspected human PaHA rescue establishes cargo delivery and pigmentation, not the positioning of whole melanosomes. The specific mouse organelle-localization experiment remains unverified. supported_by: - reference_id: PMID:17182842 - term: id: GO:0032402 label: melanosome transport evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: involved_in supporting_entities: - PANTHER:PTN001263482 - UniProtKB:Q9UL45 review: summary: Whole-melanosome movement is not resolved by the inspected cargo-routing experiments. action: UNDECIDED reason: GO:0032402 describes directed movement of whole melanosomes; inspected pallidin rescue experiments measure Tyrp1 cargo delivery to melanosomes, already represented by GO:0035646. The target self-entry is valid descendant evidence, not circularity. Neither the complete PAINT ancestral placement nor the entire experimental basis for whole-organelle movement was inspected. Retain UNDECIDED; do not replace this assertion with a duplicate cargo term or infer that the curator’s complete evidence contained no movement assay. supported_by: - reference_id: PMID:17182842 - term: id: GO:0032402 label: melanosome transport evidence_type: IDA original_reference_id: PMID:17182842 qualifier: involved_in review: summary: Whole-melanosome movement is not resolved by the inspected pallidin rescue experiment. action: UNDECIDED reason: Selected PMID:17182842 Methods, Results and Discussion establish restoration of Tyrp1 cargo routing and pigmentation in pallid melanocytes complemented with human pallidin. These endpoints do not by themselves measure directed movement of whole melanosomes. Complete figure images and supplementary material were not inspected, leaving the experimental IDA assertion unresolved. Retain UNDECIDED rather than remove it from incomplete evidence or duplicate the separately accepted cargo-delivery annotation. supported_by: - reference_id: PMID:17182842 - term: id: GO:0032438 label: melanosome organization evidence_type: NAS original_reference_id: PMID:15102850 qualifier: involved_in review: summary: Pallidin-containing BLOC-1 supports melanosome organization. action: ACCEPT reason: Complex assembly and human PaHA rescue of pallid melanocytes support melanosome biogenesis through delivery of membrane cargo. The retained NAS annotations are consistent with this mechanism and do not assert that pallidin synthesizes pigment or moves whole organelles. supported_by: - reference_id: PMID:15102850 - reference_id: PMID:17182842 - reference_id: PMID:22203680 - term: id: GO:0032438 label: melanosome organization evidence_type: NAS original_reference_id: PMID:22203680 qualifier: involved_in review: summary: Pallidin-containing BLOC-1 supports melanosome organization. action: ACCEPT reason: Complex assembly and human PaHA rescue of pallid melanocytes support melanosome biogenesis through delivery of membrane cargo. The retained NAS annotations are consistent with this mechanism and do not assert that pallidin synthesizes pigment or moves whole organelles. supported_by: - reference_id: PMID:15102850 - reference_id: PMID:17182842 - reference_id: PMID:22203680 - term: id: GO:0035264 label: multicellular organism growth evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific multicellular organism growth assertion requires its unresolved source context. action: UNDECIDED reason: The exact mouse organismal-growth assay remains unverified. Early neuronal and pigment phenotypes do not settle a whole-organism growth mechanism. - term: id: GO:0035641 label: locomotory exploration behavior evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific locomotory exploration behavior assertion requires its unresolved source context. action: UNDECIDED reason: The precise exploratory-behavior assay in pallid mice has not been independently verified; vestibular, locomotor and cognitive endpoints are not interchangeable. - term: id: GO:0035646 label: endosome to melanosome transport evidence_type: IDA original_reference_id: PMID:17182842 qualifier: involved_in review: summary: Pallidin supports transport of cargo from endosomes to melanosomes. action: ACCEPT reason: Selected PMID:17182842 experiments directly compare pallid melanocytes with human PaHA rescue and measure Tyrp1 surface/endosomal accumulation versus delivery to melanosomes. This matches cargo movement between compartments. The 2017 correction invalidates the original transferrin-FITC control; corrected recycling measurements do not overturn this cargo-sorting result. supported_by: - reference_id: PMID:17182842 - term: id: GO:0035646 label: endosome to melanosome transport evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: Pallidin supports transport of cargo from endosomes to melanosomes. action: ACCEPT reason: Selected PMID:17182842 experiments directly compare pallid melanocytes with human PaHA rescue and measure Tyrp1 surface/endosomal accumulation versus delivery to melanosomes. This matches cargo movement between compartments. The 2017 correction invalidates the original transferrin-FITC control; corrected recycling measurements do not overturn this cargo-sorting result. supported_by: - reference_id: PMID:17182842 - term: id: GO:0035651 label: AP-3 adaptor complex binding evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: enables supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific AP-3 adaptor complex binding assertion requires its unresolved source context. action: UNDECIDED reason: The inspected human recombinant BLOC-1 octamer binds AP-3, and pallidin-containing cell complexes associate with AP-3 machinery. Those experiments do not isolate a pallidin–AP-3 interface; the exact mouse donor binding assay remains unresolved. - term: id: GO:0036064 label: ciliary basal body evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: is_active_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific ciliary basal body assertion requires its unresolved source context. action: UNDECIDED reason: The specific pallidin basal-body localization experiment was not recovered. Ciliary trafficking by other BLOC-1 components cannot replace target-specific evidence. - term: id: GO:0036269 label: swimming behavior evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific swimming behavior assertion requires its unresolved source context. action: UNDECIDED reason: Pallid mice have an explicit otolith phenotype, but the precise swimming assay used for this mouse GO transfer was not independently verified. A plausible vestibular explanation does not establish the exact behavioral endpoint. - term: id: GO:0036466 label: synaptic vesicle recycling via endosome evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: involved_in supporting_entities: - FB:FBgn0036192 - PANTHER:PTN001263482 review: summary: Pallidin supports activity-dependent synaptic-vesicle recycling in the curated ancestral context. action: KEEP_AS_NON_CORE reason: The IBA uses Drosophila Pldn (FBgn0036192). Independently inspected official primary evidence for PMID:28317021 describes activity-dependent recycling-pool failure and endosomal abnormalities in fly pldn mutants, with relatively preserved basal transmission. Retain this neuronal specialization as non-core; the complete PAINT tree was not reanalyzed and the donor reference-to-node join was not reconstructed. No autonomous vesicle motor is implied. - term: id: GO:0042745 label: circadian sleep/wake cycle evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific circadian sleep/wake cycle assertion requires its unresolved source context. action: UNDECIDED reason: The exact mouse circadian sleep/wake experiment remains unverified. General behavioral or neurotransmitter changes cannot be substituted for a circadian measurement. - term: id: GO:0042802 label: identical protein binding evidence_type: IPI original_reference_id: PMID:15102850 qualifier: enables supporting_entities: - UniProtKB:Q9UL45 review: summary: Pallidin can self-associate in the tested interaction systems. action: KEEP_AS_NON_CORE reason: Selected PMID:12019270 experiments map human pallidin self-association to its coiled-coil-containing region, and other source interaction records retain self-binding. The complete BLOC-1 octamer has one copy of each subunit; that physiological stoichiometry does not refute isolated self-association assays. Retain these experimental observations as non-core rather than infer a pallidin homodimer as the functional BLOC-1 unit. supported_by: - reference_id: PMID:12019270 - reference_id: PMID:12191018 - reference_id: PMID:22203680 - term: id: GO:0042802 label: identical protein binding evidence_type: IPI original_reference_id: PMID:16189514 qualifier: enables supporting_entities: - UniProtKB:Q9UL45 review: summary: Pallidin can self-associate in the tested interaction systems. action: KEEP_AS_NON_CORE reason: Selected PMID:12019270 experiments map human pallidin self-association to its coiled-coil-containing region, and other source interaction records retain self-binding. The complete BLOC-1 octamer has one copy of each subunit; that physiological stoichiometry does not refute isolated self-association assays. Retain these experimental observations as non-core rather than infer a pallidin homodimer as the functional BLOC-1 unit. supported_by: - reference_id: PMID:12019270 - reference_id: PMID:12191018 - reference_id: PMID:22203680 - term: id: GO:0042802 label: identical protein binding evidence_type: IPI original_reference_id: PMID:25416956 qualifier: enables supporting_entities: - UniProtKB:Q9UL45 review: summary: Pallidin can self-associate in the tested interaction systems. action: KEEP_AS_NON_CORE reason: Selected PMID:12019270 experiments map human pallidin self-association to its coiled-coil-containing region, and other source interaction records retain self-binding. The complete BLOC-1 octamer has one copy of each subunit; that physiological stoichiometry does not refute isolated self-association assays. Retain these experimental observations as non-core rather than infer a pallidin homodimer as the functional BLOC-1 unit. supported_by: - reference_id: PMID:12019270 - reference_id: PMID:12191018 - reference_id: PMID:22203680 - term: id: GO:0042803 label: protein homodimerization activity evidence_type: IDA original_reference_id: PMID:12019270 qualifier: enables review: summary: Pallidin can self-associate in the tested interaction systems. action: KEEP_AS_NON_CORE reason: Selected PMID:12019270 experiments map human pallidin self-association to its coiled-coil-containing region, and other source interaction records retain self-binding. The complete BLOC-1 octamer has one copy of each subunit; that physiological stoichiometry does not refute isolated self-association assays. Retain these experimental observations as non-core rather than infer a pallidin homodimer as the functional BLOC-1 unit. supported_by: - reference_id: PMID:12019270 - reference_id: PMID:12191018 - reference_id: PMID:22203680 - term: id: GO:0042803 label: protein homodimerization activity evidence_type: IDA original_reference_id: PMID:12191018 qualifier: enables review: summary: Pallidin can self-associate in the tested interaction systems. action: KEEP_AS_NON_CORE reason: Selected PMID:12019270 experiments map human pallidin self-association to its coiled-coil-containing region, and other source interaction records retain self-binding. The complete BLOC-1 octamer has one copy of each subunit; that physiological stoichiometry does not refute isolated self-association assays. Retain these experimental observations as non-core rather than infer a pallidin homodimer as the functional BLOC-1 unit. supported_by: - reference_id: PMID:12019270 - reference_id: PMID:12191018 - reference_id: PMID:22203680 - term: id: GO:0043197 label: dendritic spine evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: is_active_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific dendritic spine assertion requires its unresolved source context. action: UNDECIDED reason: The inspected neonatal neurite and cargo-distribution studies do not independently establish pallidin localization at dendritic spines; the actual donor localization remains unverified. - term: id: GO:0043588 label: skin development evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific skin development assertion requires its unresolved source context. action: UNDECIDED reason: Pigment dilution and melanosomal defects are established, but these do not themselves resolve a skin-development process. The specific developmental donor assay remains unverified. - term: id: GO:0043679 label: axon terminus evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: is_active_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific axon terminus assertion requires its unresolved source context. action: UNDECIDED reason: Neuronal cargo reaches axonal terminals through BLOC-1-dependent routes, but cargo depletion is not itself direct localization of pallidin at the terminus. The exact mouse donor location assay remains unverified. - term: id: GO:0044291 label: cell-cell contact zone evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: is_active_in supporting_entities: - MGI:MGI:1927580 - PANTHER:PTN001583105 review: summary: The specific cell-cell contact zone assertion requires its unresolved source context. action: UNDECIDED reason: The actual mouse cell-contact localization and PAINT node evidence were not reconstructed. F-actin association and complex assembly do not settle this spatially specific term; the IBA is not discounted because of its donor count. - term: id: GO:0044291 label: cell-cell contact zone evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: is_active_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific cell-cell contact zone assertion requires its unresolved source context. action: UNDECIDED reason: The exact mouse cell-cell-contact localization assay remains unverified. The human cytoplasmic/perinuclear observations concern a different spatial resolution. - term: id: GO:0044297 label: cell body evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: is_active_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific cell body assertion requires its unresolved source context. action: UNDECIDED reason: The protein-trafficking study measures cargo distribution between neuronal cell bodies and neurites; it does not by itself locate endogenous pallidin in the exact donor context. The source localization assay remains unverified. - term: id: GO:0046085 label: adenosine metabolic process evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: Altered metabolite abundance does not establish direct participation of pallidin in adenosine metabolic process. action: MARK_AS_OVER_ANNOTATED reason: PMID:28701731 compares postnatal day 1–2 pallid and control mouse hippocampi by untargeted metabolomics and reports altered metabolite pools and increased SNAT1 abundance. The selected Results, Table 1, Discussion and Methods support a downstream metabolic consequence of BLOC-1 deficiency; they do not demonstrate pallidin performing a reaction or supplying a metabolic scaffold or cofactor. This distinction concerns participation in the named metabolic process, not merely absence of catalytic activity. The exact current mouse donor GO-row/reference edge remains unresolved, so this study is independent corroboration rather than a claimed reconstruction of that transfer. supported_by: - reference_id: PMID:28701731 - term: id: GO:0048066 label: developmental pigmentation evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: Pallidin deficiency alters pigmentation through organelle trafficking. action: KEEP_AS_NON_CORE reason: Pallid mice show pigment dilution, and human PaHA restores pigmentation in established mutant melanocytes. Retain the pigmentation phenotypes inferred from the mouse ortholog as non-core consequences of melanosomal cargo delivery, without assigning pigment synthesis chemistry to pallidin. supported_by: - reference_id: PMID:10610180 - reference_id: PMID:17182842 - term: id: GO:0048069 label: eye pigmentation evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: Pallidin deficiency alters pigmentation through organelle trafficking. action: KEEP_AS_NON_CORE reason: Pallid mice show pigment dilution, and human PaHA restores pigmentation in established mutant melanocytes. Retain the pigmentation phenotypes inferred from the mouse ortholog as non-core consequences of melanosomal cargo delivery, without assigning pigment synthesis chemistry to pallidin. supported_by: - reference_id: PMID:10610180 - reference_id: PMID:17182842 - term: id: GO:0048286 label: lung alveolus development evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific lung alveolus development assertion requires its unresolved source context. action: UNDECIDED reason: The exact mouse alveolar-development experiment remains unverified. Emphysema and alveolar maintenance phenotypes must be distinguished from developmental formation. - term: id: GO:0048490 label: anterograde synaptic vesicle transport evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: BLOC-1 supports delivery of selected neuronal membrane cargo. action: KEEP_AS_NON_CORE reason: Selected PMID:21998198 experiments include pallid mouse fibroblasts and dentate-gyrus cargo distribution, together with human neuronal-cell complexes containing endogenous pallidin. These support the neuronal trafficking pathway and the curator-mediated orthology inferences, rather than pallidin acting as an axonal motor or transporting every mature synaptic vesicle. Keep this neuronal specialization as non-core. supported_by: - reference_id: PMID:21998198 - reference_id: UniProt:Q9UL45 - term: id: GO:0048490 label: anterograde synaptic vesicle transport evidence_type: ISS original_reference_id: GO_REF:0000024 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 review: summary: BLOC-1 supports delivery of selected neuronal membrane cargo. action: KEEP_AS_NON_CORE reason: Selected PMID:21998198 experiments include pallid mouse fibroblasts and dentate-gyrus cargo distribution, together with human neuronal-cell complexes containing endogenous pallidin. These support the neuronal trafficking pathway and the curator-mediated orthology inferences, rather than pallidin acting as an axonal motor or transporting every mature synaptic vesicle. Keep this neuronal specialization as non-core. supported_by: - reference_id: PMID:21998198 - reference_id: UniProt:Q9UL45 - term: id: GO:0048813 label: dendrite morphogenesis evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific dendrite morphogenesis assertion requires its unresolved source context. action: UNDECIDED reason: Pallid primary neurons have a modest neurite-extension phenotype, but the inspected experiments do not independently resolve a dendrite-specific morphogenesis step. The exact donor morphology assay remains unverified. - term: id: GO:0048840 label: otolith development evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: Pallidin loss disrupts otolith formation in mice. action: KEEP_AS_NON_CORE reason: The complete normal PMID:10610180 abstract explicitly identifies abnormal otolith formation among pallid mouse phenotypes. This provides positive target-relevant support for retaining the existing developmental transfer as non-core. It is not evidence for a direct human calcium-deposition enzyme or an isolated otolith-assembly reaction. supported_by: - reference_id: PMID:10610180 - term: id: GO:0050942 label: positive regulation of pigment cell differentiation evidence_type: IDA original_reference_id: PMID:17182842 qualifier: involved_in review: summary: The specific positive regulation of pigment cell differentiation assertion requires its unresolved source context. action: UNDECIDED reason: GO:0050942 concerns acquisition of a differentiated pigment-cell state. The selected human PaHA experiments restore pigment and cargo sorting in established melanocyte lines, not necessarily differentiation. The precise curator basis may include material not inspected; retain the experimental assertion as undecided. supported_by: - reference_id: PMID:17182842 - term: id: GO:0051015 label: actin filament binding evidence_type: IDA original_reference_id: PMID:12019270 qualifier: enables review: summary: Pallidin-containing material associates with filamentous actin. action: KEEP_AS_NON_CORE reason: Selected PMID:12019270 methods and results show pallidin-containing cytosolic material sedimenting with purified F-actin and tagged human pallidin at perinuclear actin structures. The authors explicitly leave direct binding versus mediation by other actin-associated proteins unresolved. Preserve the experimental and transferred association as non-core without assigning pallidin an actin-polymerizing or crosslinking mechanism; the filamentous-actin part_of assertion is read as association, not incorporation as an actin monomer. supported_by: - reference_id: PMID:12019270 - term: id: GO:0051015 label: actin filament binding evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: enables supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: Pallidin-containing material associates with filamentous actin. action: KEEP_AS_NON_CORE reason: Selected PMID:12019270 methods and results show pallidin-containing cytosolic material sedimenting with purified F-actin and tagged human pallidin at perinuclear actin structures. The authors explicitly leave direct binding versus mediation by other actin-associated proteins unresolved. Preserve the experimental and transferred association as non-core without assigning pallidin an actin-polymerizing or crosslinking mechanism; the filamentous-actin part_of assertion is read as association, not incorporation as an actin monomer. supported_by: - reference_id: PMID:12019270 - term: id: GO:0051639 label: actin filament network formation evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific actin filament network formation assertion requires its unresolved source context. action: UNDECIDED reason: The selected actin study establishes association with F-actin but leaves direct versus mediated binding unresolved. It does not demonstrate a pallidin-driven filament-network assembly reaction, and the mouse donor experiment remains unverified. supported_by: - reference_id: PMID:12019270 - term: id: GO:0055088 label: lipid homeostasis evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific lipid homeostasis assertion requires its unresolved source context. action: UNDECIDED reason: A 2005 primary Regensburg thesis reports altered lipid profiles in pooled sera from four pallidin-knockout and four control mice. This is positive target-relevant evidence, but the limited pooled comparison, proposed rather than established maintenance mechanism, and unresolved current donor-source edge leave the specific homeostasis assignment undecided. It is not evidence against a lipid role. - term: id: GO:0060090 label: molecular adaptor activity evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: enables supporting_entities: - MGI:MGI:1927580 - PANTHER:PTN001583105 review: summary: Pallidin contributes to the BLOC-1 molecular assembly that coordinates trafficking proteins. action: ACCEPT reason: GO:0060090 concerns stoichiometric association of two or more proteins to coordinate their function. The inspected human reconstitution identifies pallidin contacts with both BLOS1 and Cappuccino in a stable ternary assembly, within the functional BLOC-1 octamer. This supports an adaptor/scaffold interpretation without an autonomous motor or enzyme claim. The curated IBA is retained; donor count is not used to challenge its ancestral node. supported_by: - reference_id: PMID:22203680 - reference_id: PMID:12019270 - term: id: GO:0060090 label: molecular adaptor activity evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: enables supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: Pallidin contributes to the BLOC-1 molecular assembly that coordinates trafficking proteins. action: ACCEPT reason: GO:0060090 concerns stoichiometric association of two or more proteins to coordinate their function. The inspected human reconstitution identifies pallidin contacts with both BLOS1 and Cappuccino in a stable ternary assembly, within the functional BLOC-1 octamer. This supports an adaptor/scaffold interpretation without an autonomous motor or enzyme claim. The curated IBA is retained; donor count is not used to challenge its ancestral node. supported_by: - reference_id: PMID:22203680 - reference_id: PMID:12019270 - term: id: GO:0060155 label: platelet dense granule organization evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: Pallidin-containing BLOC-1 contributes to platelet dense-granule organization. action: ACCEPT reason: The normal pallid-gene abstract explicitly links loss of pallidin to platelet storage-pool deficiency, and the human BLOC-1 assembly studies place it in lysosome-related-organelle biogenesis. Retain this organelle role. The retraction of the 2011 human HPS9 paper does not invalidate the independent mouse evidence or all subsequent human disease reports. supported_by: - reference_id: PMID:10610180 - reference_id: PMID:12019270 - reference_id: UniProt:Q9UL45 - term: id: GO:0070328 label: triglyceride homeostasis evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific triglyceride homeostasis assertion requires its unresolved source context. action: UNDECIDED reason: The selected 2005 primary thesis reports reduced triglycerides in separated VLDL fractions from pooled pallidin-knockout mouse sera. The actual current donor citation and a triglyceride-maintenance mechanism remain unresolved; proposed secretion or LCAT explanations are not pallidin catalytic evidence. Preserve the source as undecided rather than deny the reported biochemical phenotype. - term: id: GO:0070938 label: contractile ring evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: is_active_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: Specific localization to the cytokinetic contractile ring remains unresolved. action: UNDECIDED reason: GO:0070938 describes a cytoskeletal ring on the cytoplasmic side of the membrane. Pallidin’s cytosolic and actin-associated properties are compatible with that topology, unlike an ER-lumen claim, but do not establish localization to the cytokinetic ring. The exact mouse localization experiment remains unverified, so this electronic annotation remains UNDECIDED. - term: id: GO:0071364 label: cellular response to epidermal growth factor stimulus evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific cellular response to epidermal growth factor stimulus assertion requires its unresolved source context. action: UNDECIDED reason: The exact mouse EGF-response experiment remains unverified; no receptor-signalling function is inferred solely from endosomal trafficking. - term: id: GO:0072657 label: protein localization to membrane evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific protein localization to membrane assertion requires its unresolved source context. action: UNDECIDED reason: The precise mouse protein-to-membrane localization assay remains unverified. BLOC-1 cargo delivery is established, but this general term could cover distinct cargo and membrane contexts. - term: id: GO:0072659 label: protein localization to plasma membrane evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific protein localization to plasma membrane assertion requires its unresolved source context. action: UNDECIDED reason: The precise mouse plasma-membrane cargo assay remains unverified. Tyrp1 accumulation at the surface of pallid melanocytes is a missorting phenotype, not evidence that pallidin directly delivers that cargo to the plasma membrane. supported_by: - reference_id: PMID:17182842 - term: id: GO:0097006 label: regulation of plasma lipoprotein particle levels evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific regulation of plasma lipoprotein particle levels assertion requires its unresolved source context. action: UNDECIDED reason: Selected primary mouse thesis data concern lipid content in separated plasma fractions, including altered HDL lipids and VLDL triglycerides. These composition measurements do not by themselves establish regulation of lipoprotein particle numbers. The current donor-source edge and adequate particle-level assay remain unresolved; retain UNDECIDED. - term: id: GO:0097487 label: multivesicular body, internal vesicle evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: is_active_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific multivesicular body, internal vesicle assertion requires its unresolved source context. action: UNDECIDED reason: BLOC-1 acts at cytoplasmic endosomal surfaces, but that does not establish pallidin within internal intraluminal vesicles. The exact donor ultrastructural or biochemical evidence remains unverified. - term: id: GO:0098975 label: postsynapse of neuromuscular junction evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: is_active_in supporting_entities: - FB:FBgn0036192 - PANTHER:PTN001263482 review: summary: The fly pallidin donor is detected on the postsynaptic side of the neuromuscular junction. action: KEEP_AS_NON_CORE reason: Official primary evidence for PMID:28317021 includes antibody localization in postsynaptic muscle as well as presynaptic neuronal structures. This supports the source fly donor context, and the ancestral IBA is retained as non-core. It is not presented as direct localization of endogenous human pallidin or as a newly reconstructed PAINT node assignment. - term: id: GO:0099640 label: axo-dendritic protein transport evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: BLOC-1 supports delivery of selected neuronal membrane cargo. action: KEEP_AS_NON_CORE reason: Selected PMID:21998198 experiments include pallid mouse fibroblasts and dentate-gyrus cargo distribution, together with human neuronal-cell complexes containing endogenous pallidin. These support the neuronal trafficking pathway and the curator-mediated orthology inferences, rather than pallidin acting as an axonal motor or transporting every mature synaptic vesicle. Keep this neuronal specialization as non-core. supported_by: - reference_id: PMID:21998198 - reference_id: UniProt:Q9UL45 - term: id: GO:1903441 label: protein localization to ciliary membrane evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: involved_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific protein localization to ciliary membrane assertion requires its unresolved source context. action: UNDECIDED reason: The exact pallidin-dependent ciliary-membrane cargo assay remains unverified. Results for other BLOC-1 subunits do not by themselves establish the target-specific step. - term: id: GO:1904115 label: axon cytoplasm evidence_type: IEA original_reference_id: GO_REF:0000108 qualifier: located_in supporting_entities: - GO:0008089 review: summary: An axonal cytoplasmic context is consistent with neuronal cargo trafficking. action: KEEP_AS_NON_CORE reason: This is the logical cellular-location consequence of the retained anterograde axonal-transport annotation. Selected mouse neuronal cargo experiments support the pathway, but do not independently image endogenous human pallidin throughout the axoplasm. Retain the inference as non-core. supported_by: - reference_id: PMID:21998198 - term: id: GO:1990742 label: microvesicle evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: is_active_in supporting_entities: - UniProtKB:Q9R0C0 - ensembl:ENSMUSP00000005954 review: summary: The specific microvesicle assertion requires its unresolved source context. action: UNDECIDED reason: GO:1990742 denotes extracellular microvesicles. The inspected PMID:21998198 small-vesicle preparation is made from homogenized PC12 cells and concerns intracellular vesicles. This distinction does not prove the donor is wrong, but the actual extracellular-vesicle localization evidence remains unverified. references: - id: GO_REF:0000024 title: Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity findings: - statement: This reference identifies the original annotation inference method; each source row retains its exact evidence, qualifier and supporting entities. - id: GO_REF:0000033 title: Annotation inferences using phylogenetic trees findings: - statement: This reference identifies the original annotation inference method; each source row retains its exact evidence, qualifier and supporting entities. - id: GO_REF:0000044 title: Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt findings: - statement: This reference identifies the original annotation inference method; each source row retains its exact evidence, qualifier and supporting entities. - id: GO_REF:0000107 title: Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara findings: - statement: This reference identifies the original annotation inference method; each source row retains its exact evidence, qualifier and supporting entities. - id: GO_REF:0000108 title: Automatic assignment of GO terms using logical inference, based on on inter-ontology links findings: - statement: This reference identifies the original annotation inference method; each source row retains its exact evidence, qualifier and supporting entities. - id: GO_REF:0000117 title: Electronic Gene Ontology annotations created by ARBA machine learning models findings: - statement: This reference identifies the original annotation inference method; each source row retains its exact evidence, qualifier and supporting entities. - id: GO_REF:0000120 title: Combined Automated Annotation using Multiple IEA Methods findings: - statement: This reference identifies the original annotation inference method; each source row retains its exact evidence, qualifier and supporting entities. - id: PMID:10610180 title: The pallid gene encodes a novel, syntaxin 13-interacting protein involved in platelet storage pool deficiency. findings: - statement: Pallid mouse phenotypes include platelet storage-pool deficiency, pigment dilution and abnormal otolith formation; pallidin interacts with syntaxin 13. full_text_unavailable: true reference_review: relevance: HIGH correctness: VERIFIED review_notes: Complete canonical normal abstract read. It reports two-hybrid and co-immunoprecipitation support, but the full article and all species-specific constructs were not inspected. - id: PMID:12019270 title: BLOC-1, a novel complex containing the pallidin and muted proteins involved in the biogenesis of melanosomes and platelet-dense granules. findings: - statement: Human pallidin has soluble and peripheral membrane pools, self-associates and occurs in BLOC-1; pallidin-containing cytosol associates with F-actin. full_text_unavailable: true reference_review: relevance: HIGH correctness: VERIFIED review_notes: Canonical normal cache is abstract-only. Separately indexed original-paper Methods/Results/Discussion passages were read, including human constructs and F-actin sedimentation. The paper explicitly leaves direct versus mediated actin binding unresolved. No full figure/supplement claim. - id: PMID:12191018 title: Pallidin is a component of a multi-protein complex involved in the biogenesis of lysosome-related organelles. findings: - statement: Human pallidin is a component of a heterooligomer containing Muted; muted mouse cells have reduced pallidin. full_text_unavailable: true reference_review: relevance: HIGH correctness: VERIFIED review_notes: Complete canonical abstract read. Exact pair-level assays and full paper were not independently inspected. - id: PMID:15102850 title: Identification of snapin and three novel proteins (BLOS1, BLOS2, and BLOS3/reduced pigmentation) as subunits of biogenesis of lysosome-related organelles complex-1 (BLOC-1). findings: - statement: Endogenous complex biochemistry and interaction analysis identify the eight-subunit BLOC-1 assembly. full_text_unavailable: true reference_review: relevance: HIGH correctness: VERIFIED review_notes: Complete canonical abstract read; no independent reading of every two-hybrid pair matrix. - id: PMID:16189514 title: Towards a proteome-scale map of the human protein-protein interaction network. findings: - statement: A human interaction map supplies curated pallidin partner and self-interaction records. full_text_unavailable: true reference_review: relevance: MEDIUM correctness: VERIFIED review_notes: Canonical abstract and assay scope read; individual supplementary interaction rows were not independently inspected. - id: PMID:17182842 title: BLOC-1 is required for cargo-specific sorting from vacuolar early endosomes toward lysosome-related organelles. findings: - statement: Human PaHA restores pigmentation and Tyrp1 cargo routing in pallid mouse melanocytes. full_text_unavailable: true reference_review: relevance: HIGH correctness: VERIFIED review_notes: Canonical cache remains abstract-only. Selected separately archived full-text HTML Methods/Results/Discussion were read separately, including pallid rescue and Tyrp1 routing; no complete figures or supplementary images were inspected. The 2017 correction (PMID:28137951) invalidates the original transferrin-FITC control and reports a mild early recycling defect with corrected reagent, while retaining the central cargo conclusion. Whole-melanosome movement and pigment-cell differentiation are not resolved by the inspected rescue alone. The complete separately cached correction PMID:28137951 was subsequently read; the original cache remains unchanged. - id: PMID:19546860 title: 'The dysbindin-containing complex (BLOC-1) in brain: developmental regulation, interaction with SNARE proteins and role in neurite outgrowth.' findings: - statement: Native brain BLOC-1 associates selectively with SNARE proteins; pallid mouse primary neurons have modest neurite-extension defects. full_text_unavailable: false reference_review: relevance: HIGH correctness: VERIFIED review_notes: 'Canonical full text: selected complete relevant Methods, Results and Discussion read. Native-complex anti-pallidin detection is distinguished from isolated pallidin binding; no whole-paper image coverage claimed. Bovine brain native-complex pull-downs are distinguished from source-listed human or mouse recombinant SNARE partners; this refines the existing association class without mapping each direct pallidin contact.' - id: PMID:21998198 title: The schizophrenia susceptibility factor dysbindin and its associated complex sort cargoes from cell bodies to the synapse. findings: - statement: Pallidin-containing BLOC-1 participates in neuronal cargo sorting and AP-3-associated complexes. full_text_unavailable: false reference_review: relevance: HIGH correctness: VERIFIED review_notes: 'Canonical full text: selected Results and Methods read, including actual pallid mouse experiments and human SH-SY5Y complex assays. Other experiments use muted, dysbindin or AP-3 mutants. Intracellular PC12 microvesicle preparations are not extracellular microvesicles.' - id: PMID:22203680 title: Assembly and architecture of biogenesis of lysosome-related organelles complex-1 (BLOC-1). findings: - statement: Human pallidin, Cappuccino and BLOS1 form a stable subassembly within the reconstituted BLOC-1 octamer. full_text_unavailable: true reference_review: relevance: HIGH correctness: VERIFIED review_notes: Canonical cache is abstract-only. Selected separately archived full-text HTML Methods/Results/Discussion were read separately, including isolated pair and trimer reconstitution. Whole-octamer AP-3 pull-down does not map an isolated pallidin–AP-3 interface; no figure-image inspection claimed. - id: PMID:25416956 title: A proteome-scale map of the human interactome network. findings: - statement: A systematic human binary interactome supplies curated pallidin physical associations. full_text_unavailable: false reference_review: relevance: MEDIUM correctness: VERIFIED review_notes: Complete canonical abstract read; exact supplementary partner rows not independently inspected. - id: PMID:29892012 title: An interactome perturbation framework prioritizes damaging missense mutations for developmental disorders. findings: - statement: Human interaction perturbation experiments provide source-level physical-interaction context. full_text_unavailable: false reference_review: relevance: MEDIUM correctness: VERIFIED review_notes: Complete canonical abstract read; no independent reproduction of the pallidin pair-level measurement. - id: PMID:31515488 title: Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations. findings: - statement: A population-variant interaction study supplies curated pallidin interaction observations. full_text_unavailable: false reference_review: relevance: MEDIUM correctness: VERIFIED review_notes: Complete canonical abstract read; exact partner-level dataset not independently inspected. - id: PMID:32296183 title: A reference map of the human binary protein interactome. findings: - statement: HuRI supplies human binary-interaction evidence for source-listed pallidin partners. full_text_unavailable: false reference_review: relevance: MEDIUM correctness: VERIFIED review_notes: Complete canonical abstract read; individual supplementary rows not independently inspected. - id: PMID:33961781 title: Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. findings: - statement: BioPlex maps physical associations in human cell lines. full_text_unavailable: false reference_review: relevance: MEDIUM correctness: VERIFIED review_notes: Complete canonical abstract read; AP-MS co-purification is not automatically direct binary binding and pair-level supplements were not independently read. - id: PMID:40205054 title: Multimodal cell maps as a foundation for structural and functional genomics. findings: - statement: A multimodal human cell map supplies the pallidin–dysbindin association. full_text_unavailable: false reference_review: relevance: MEDIUM correctness: VERIFIED review_notes: Canonical abstract read; pair-level dataset not independently inspected. Official correction PMID:41039152 corrects a duplicated loss-function equation and does not identify a change to this BLOC-1 interaction record; no broader revalidation is claimed. Existing canonical correction PMID:41039152 remains protected. - id: Reactome:R-HSA-429815 title: Formation Of Bloc-1 Complex findings: - statement: Reactome describes assembly of the eight-subunit BLOC-1 complex, including pallidin. reference_review: relevance: HIGH correctness: VERIFIED review_notes: Normal cached event summary read. Independent human biochemical evidence supports the cytosolic pool; the event title alone is not treated as a localization assay. - id: UniProt:Q9UL45 title: 'UniProtKB Q9UL45 (BL1S6_HUMAN): BLOC1S6' findings: - statement: Human pallidin is a BLOC-1 subunit with three alternative products and recorded physical interactions. supporting_text: Component of the BLOC-1 complex reference_review: relevance: HIGH correctness: VERIFIED review_notes: 'The human UniProt record was read, including interactions, sequence features, alternative products and the explicit warning about retracted PMID:21665000. The independent 2012 PMID:22461475 letter was checked against PubMed metadata and its author-uploaded original text: patient NK-cell degranulation, marker distribution and PLDN replacement experiments were read; no supplementary images or complete raw data were inspected. Its separate patient evidence is not invalidated merely because it cites the retracted 2011 article. The third alternative product remains Not described, with no isoform-specific function inferred.' - id: PMID:28701731 title: BLOC-1 deficiency causes alterations in amino acid profile and in phospholipid and adenosine metabolism in the postnatal mouse hippocampus. findings: - statement: Pallid mouse hippocampi show altered metabolite pools and increased SNAT1; these changes do not establish a metabolic reaction performed by pallidin. supporting_text: "28 metabolites that showed statistically\nsignificant changes" full_text_unavailable: false reference_review: relevance: HIGH correctness: VERIFIED review_notes: Canonical XML metadata and narrative abstract, selected complete metabolomic Results/Table 1 and Discussion passages, and analytical Methods were read. Postnatal day 1–2 pallid hippocampi are distinguished from human assays. The exact current donor-reference edge was not reconstructed. - id: PMID:17785837 title: Neutrophils activate macrophages for intracellular killing of Leishmania major through recruitment of TLR4 by neutrophil elastase. findings: - statement: Pallid mouse inflammatory neutrophils release less elastase and fail to promote normal macrophage parasite killing. supporting_text: defective in the spontaneous release of NE full_text_unavailable: true reference_review: relevance: HIGH correctness: VERIFIED review_notes: The canonical normal cache has a narrative abstract but no full text. Selected original Results and Methods were independently read during the earlier donor consultation. Elastase release and downstream infection endpoints support a secretion-related role; pallidin is not the protease or macrophage receptor. - id: PMID:22423091 title: Quantitative proteomic and genetic analyses of the schizophrenia susceptibility factor dysbindin identify novel roles of the biogenesis of lysosome-related organelles complex 1. findings: - statement: Human pallidin knockdown alters BLOC-1 and COG-associated proteins and increases basal oxidant signal while reducing peroxiredoxins. full_text_unavailable: false reference_review: relevance: HIGH correctness: VERIFIED review_notes: Canonical HTML metadata and abstract plus selected Results on COG association, pallidin shRNA, peroxiredoxins and basal redox state were read. The abstract foregrounds dysbindin, but the body includes human pallidin perturbation. Complex association does not identify an isolated pallidin interface, and basal redox change alone does not settle the stress-response term. - id: PMID:28317021 title: The BLOC-1 Subunit Pallidin Facilitates Activity-Dependent Synaptic Vesicle Recycling. findings: - statement: Drosophila pallidin supports synaptic vesicle recycling under sustained activity, with activity-dependent endosomal changes. full_text_unavailable: false reference_review: relevance: HIGH correctness: VERIFIED review_notes: Canonical XML metadata and abstract, selected localization/development and electrophysiology Results with figure-caption text, plus selected Discussion were read. Null flies retain normal basal architecture and transmission but show rapid vesicle-pool rundown, slow recovery and altered early-endosome morphology under strong activity. These findings corroborate trafficking; they are not human experiments or evidence against every developmental annotation. Figure images and supplemental data were not independently inspected. - id: PMID:28137951 title: Correction for BLOC-1 Is Required for Cargo-specific Sorting from Vacuolar Early Endosomes toward Lysosome-related Organelles. findings: - statement: The correction invalidates the original transferrin-FITC control and replaces it with validated transferrin recycling measurements. full_text_unavailable: false reference_review: relevance: HIGH correctness: VERIFIED review_notes: The normal Abstract section contains citation metadata only; the complete canonical XML correction text was read. The replacement assay includes melan-mu and melan-pa cells and their rescued controls, showing a mild early recycling defect. The notice states that the central cargo-sorting conclusions remain intact. This is a correction notice despite the generic machine publication-type classification. - id: PMID:22461475 title: Exome sequencing reveals a pallidin mutation in a Hermansky-Pudlak-like primary immunodeficiency syndrome. findings: - statement: A separate 2012 patient report describes impaired NK-cell degranulation and restoration of CD107a distribution after pallidin replacement. full_text_unavailable: true reference_review: relevance: HIGH correctness: VERIFIED review_notes: 'The canonical cache contains citation metadata only, not a narrative abstract or full text. Scientific assessment instead uses the separately inspected author-uploaded original Blood letter: patient findings, functional-results text and Figure 1 caption. The independent case reports normal platelet aggregation and is distinct from the retracted 2011 case. No figure-image, supplemental-data or full raw-data audit is claimed.' - id: PMID:21665000 title: A BLOC-1 mutation screen reveals that PLDN is mutated in Hermansky-Pudlak Syndrome type 9. findings: - statement: The 2011 pallidin case report is retracted and excluded from positive functional or disease evidence. full_text_unavailable: false reference_review: relevance: HIGH correctness: LOW_QUALITY review_notes: PubMed metadata explicitly marks Retracted Publication and identifies the 2017 retraction. The recovered HTML is retained for provenance, not used to validate its experiments. The retraction does not automatically invalidate the independent 2012 patient letter. is_invalid: true - id: PMID:28475864 title: 'Retraction Notice to: A BLOC-1 Mutation Screen Reveals that PLDN Is Mutated in Hermansky-Pudlak Syndrome Type 9.' findings: - statement: This notice formally retracts the 2011 pallidin case report PMID:21665000. full_text_unavailable: true reference_review: relevance: HIGH correctness: VERIFIED review_notes: The canonical record has citation metadata and an explicit Retraction of relation, with no narrative abstract or recovered full text. Official PubMed retraction provenance was checked. No detailed retraction rationale or whole-notice body reading is claimed. core_functions: - description: Pallidin binds syntaxin trafficking machinery and assembles into BLOC-1, contributing molecular adaptor activity to endosomal cargo sorting and lysosome-related organelle biogenesis. Pallidin-specific syntaxin association is supported by the original two-hybrid/co-immunoprecipitation study and the human interaction record; native brain experiments establish selective SNARE recognition by the intact complex. BLOC-1-dependent cargo delivery supports melanosome organization, endosome organization and platelet dense-granule formation, without establishing an autonomous pallidin fusion or motor activity. contributes_to_molecular_function: id: GO:0060090 label: molecular adaptor activity in_complex: id: GO:0031083 label: BLOC-1 complex locations: - id: GO:0005829 label: cytosol - id: GO:0005768 label: endosome directly_involved_in: - id: GO:0035646 label: endosome to melanosome transport - id: GO:0060155 label: platelet dense granule organization supported_by: - reference_id: PMID:10610180 - reference_id: PMID:12019270 - reference_id: PMID:17182842 - reference_id: PMID:22203680 supporting_text: stable subcomplexes were defined, pallidin-Cappuccino-BLOS1 - reference_id: UniProt:Q9UL45 knowledge_gaps: - gap_statement: How pallidin-specific interfaces couple BLOC-1 assembly to SNARE-associated cargo sorting remains incompletely resolved. boundary: Defined human pallidin–BLOS1 and pallidin–Cappuccino interfaces and functional rescue support the assembly role. Whole-complex SNARE/AP-3 association and F-actin sedimentation do not map every contact to isolated pallidin or establish actin remodelling chemistry. The interfaces and conformational requirements by which pallidin contributes to SNARE recognition in intact BLOC-1 remain incompletely mapped. Native-complex pull-downs do not assign every observed SNARE interaction to a direct isolated-pallidin interface; the coupling between recognition, cargo sorting and membrane fusion remains unresolved. gap_kind: - BIOLOGY status: OPEN resolution: Separate interface mutations that disrupt BLOC-1 stability from those that selectively alter cargo sorting or partner recognition. molecular_function: id: GO:0019905 label: syntaxin binding suggested_questions: - question: Which direct pallidin interfaces connect the BLOC-1 scaffold to syntaxins, AP-3 and actin-associated machinery without merely destabilizing the octamer? - question: Which mouse-derived developmental, spatial and metabolic annotations reflect a direct pallidin activity, and which are downstream consequences of organelle dysfunction?