Barttin, encoded by BSND, is an integral membrane accessory subunit of the ClC-Ka and ClC-Kb chloride channels. It associates with these pore-forming subunits and regulates channel activation, surface abundance and polarized membrane sorting. Barttin modifies cooperative channel gating, favoring chloride conductance at physiological potentials; its palmitoylation at Cys54 and Cys56 supports efficient activation. Trafficking and activation can be separated experimentally and by disease-associated variants. ClC-K/barttin complexes support renal chloride reabsorption and potassium recycling in the inner ear. Wild-type human barttin preferentially sorts basolaterally in polarized epithelial models. Loss of barttin function causes Bartter syndrome with sensorineural deafness, while some hypomorphic variants predominantly impair hearing.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005254 chloride channel activity | IEA GO_REF:0000107 | ACCEPT | Summary: Barttin contributes to the chloride conductance of ClC-K/barttin heteromeric channels. Reason: The preserved contributes_to qualifier is essential: barttin is an accessory regulator of ClC-K channel trafficking and function, whereas ClC-K supplies the chloride-conducting pore. Coexpression increases current and surface abundance, and disease variants disrupt channel function. The ortholog-derived assertion is consistent with target-specific evidence; it does not assign autonomous pore activity to barttin. |
| GO:0005254 chloride channel activity | ISS GO_REF:0000024 | ACCEPT | Summary: Barttin contributes to the chloride conductance of ClC-K/barttin heteromeric channels. Reason: The preserved contributes_to qualifier is essential: barttin is an accessory regulator of ClC-K channel trafficking and function, whereas ClC-K supplies the chloride-conducting pore. Coexpression increases current and surface abundance, and disease variants disrupt channel function. The ortholog-derived assertion is consistent with target-specific evidence; it does not assign autonomous pore activity to barttin. |
| GO:0005515 protein binding | IPI PMID:12111250 Barttin increases surface expression and changes current pro... | MODIFY | Summary: Barttin associates with CLCNKA as a functional chloride-channel accessory subunit. Reason: The original partner accession is preserved. The cited coexpression and co-immunoprecipitation study establishes channel activation together with association, supporting chloride channel regulator activity rather than generic binding alone. This refinement overlaps the existing regulator annotations and is not a proposed NEW assertion. The abstract does not establish an isolated purified binary-binding assay. These source objects retain distinct CLCNKA and CLCNKB partner accessions, which are not supplied by the broader regulator row. Proposed replacements: chloride channel regulator activity Supporting Evidence: PMID:12111250 revealed a direct interaction of barttin with ClC-K. file:human/BSND/BSND-uniprot.txt |
| GO:0005515 protein binding | IPI PMID:12111250 Barttin increases surface expression and changes current pro... | MODIFY | Summary: Barttin associates with CLCNKB as a functional chloride-channel accessory subunit. Reason: The original partner accession is preserved. The cited coexpression and co-immunoprecipitation study establishes channel activation together with association, supporting chloride channel regulator activity rather than generic binding alone. This refinement overlaps the existing regulator annotations and is not a proposed NEW assertion. The abstract does not establish an isolated purified binary-binding assay. These source objects retain distinct CLCNKA and CLCNKB partner accessions, which are not supplied by the broader regulator row. Proposed replacements: chloride channel regulator activity |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: HuRI reports a BSND interaction with UniProtKB:O14735. Reason: The exact source partner UniProtKB:O14735 is listed in the curated UniProt/IntAct interaction record (NbExp=3). The inspected HuRI Results describe systematic human-protein Y2H screening, quadruplicate pairwise retesting and sequence confirmation. Together these support retention as a reported experimental binding observation, with deference to curators for this pair's original supplementary record. That target record was not independently inspected; the experiment count is not treated as independent publications, and the dataset-level orthogonal validation is not claimed for this individual edge. No native physiological role, partner activity or more-specific binding function is inferred. Supported generic binding is retained as non-core under the standing project instruction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: HuRI reports a BSND interaction with UniProtKB:O95159. Reason: The exact source partner UniProtKB:O95159 is listed in the curated UniProt/IntAct interaction record (NbExp=3). The inspected HuRI Results describe systematic human-protein Y2H screening, quadruplicate pairwise retesting and sequence confirmation. Together these support retention as a reported experimental binding observation, with deference to curators for this pair's original supplementary record. That target record was not independently inspected; the experiment count is not treated as independent publications, and the dataset-level orthogonal validation is not claimed for this individual edge. No native physiological role, partner activity or more-specific binding function is inferred. Supported generic binding is retained as non-core under the standing project instruction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: HuRI reports a BSND interaction with UniProtKB:O95393. Reason: The exact source partner UniProtKB:O95393 is listed in the curated UniProt/IntAct interaction record (NbExp=3). The inspected HuRI Results describe systematic human-protein Y2H screening, quadruplicate pairwise retesting and sequence confirmation. Together these support retention as a reported experimental binding observation, with deference to curators for this pair's original supplementary record. That target record was not independently inspected; the experiment count is not treated as independent publications, and the dataset-level orthogonal validation is not claimed for this individual edge. No native physiological role, partner activity or more-specific binding function is inferred. Supported generic binding is retained as non-core under the standing project instruction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: HuRI reports a BSND interaction with UniProtKB:P24593. Reason: The exact source partner UniProtKB:P24593 is listed in the curated UniProt/IntAct interaction record (NbExp=3). The inspected HuRI Results describe systematic human-protein Y2H screening, quadruplicate pairwise retesting and sequence confirmation. Together these support retention as a reported experimental binding observation, with deference to curators for this pair's original supplementary record. That target record was not independently inspected; the experiment count is not treated as independent publications, and the dataset-level orthogonal validation is not claimed for this individual edge. No native physiological role, partner activity or more-specific binding function is inferred. Supported generic binding is retained as non-core under the standing project instruction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: HuRI reports a BSND interaction with UniProtKB:P29400-2. Reason: The exact source partner UniProtKB:P29400-2 is listed in the curated UniProt/IntAct interaction record (NbExp=3). The inspected HuRI Results describe systematic human-protein Y2H screening, quadruplicate pairwise retesting and sequence confirmation. Together these support retention as a reported experimental binding observation, with deference to curators for this pair's original supplementary record. That target record was not independently inspected; the experiment count is not treated as independent publications, and the dataset-level orthogonal validation is not claimed for this individual edge. No native physiological role, partner activity or more-specific binding function is inferred. Supported generic binding is retained as non-core under the standing project instruction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: HuRI reports a BSND interaction with UniProtKB:P43378. Reason: The exact source partner UniProtKB:P43378 is listed in the curated UniProt/IntAct interaction record (NbExp=3). The inspected HuRI Results describe systematic human-protein Y2H screening, quadruplicate pairwise retesting and sequence confirmation. Together these support retention as a reported experimental binding observation, with deference to curators for this pair's original supplementary record. That target record was not independently inspected; the experiment count is not treated as independent publications, and the dataset-level orthogonal validation is not claimed for this individual edge. No native physiological role, partner activity or more-specific binding function is inferred. Supported generic binding is retained as non-core under the standing project instruction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: HuRI reports a BSND interaction with UniProtKB:P53367. Reason: The exact source partner UniProtKB:P53367 is listed in the curated UniProt/IntAct interaction record (NbExp=5). The inspected HuRI Results describe systematic human-protein Y2H screening, quadruplicate pairwise retesting and sequence confirmation. Together these support retention as a reported experimental binding observation, with deference to curators for this pair's original supplementary record. That target record was not independently inspected; the experiment count is not treated as independent publications, and the dataset-level orthogonal validation is not claimed for this individual edge. No native physiological role, partner activity or more-specific binding function is inferred. Supported generic binding is retained as non-core under the standing project instruction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: HuRI reports a BSND interaction with UniProtKB:Q05329. Reason: The exact source partner UniProtKB:Q05329 is listed in the curated UniProt/IntAct interaction record (NbExp=3). The inspected HuRI Results describe systematic human-protein Y2H screening, quadruplicate pairwise retesting and sequence confirmation. Together these support retention as a reported experimental binding observation, with deference to curators for this pair's original supplementary record. That target record was not independently inspected; the experiment count is not treated as independent publications, and the dataset-level orthogonal validation is not claimed for this individual edge. No native physiological role, partner activity or more-specific binding function is inferred. Supported generic binding is retained as non-core under the standing project instruction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: HuRI reports a BSND interaction with UniProtKB:Q15836. Reason: The exact source partner UniProtKB:Q15836 is listed in the curated UniProt/IntAct interaction record (NbExp=3). The inspected HuRI Results describe systematic human-protein Y2H screening, quadruplicate pairwise retesting and sequence confirmation. Together these support retention as a reported experimental binding observation, with deference to curators for this pair's original supplementary record. That target record was not independently inspected; the experiment count is not treated as independent publications, and the dataset-level orthogonal validation is not claimed for this individual edge. No native physiological role, partner activity or more-specific binding function is inferred. Supported generic binding is retained as non-core under the standing project instruction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: HuRI reports a BSND interaction with UniProtKB:Q15848. Reason: The exact source partner UniProtKB:Q15848 is listed in the curated UniProt/IntAct interaction record (NbExp=3). The inspected HuRI Results describe systematic human-protein Y2H screening, quadruplicate pairwise retesting and sequence confirmation. Together these support retention as a reported experimental binding observation, with deference to curators for this pair's original supplementary record. That target record was not independently inspected; the experiment count is not treated as independent publications, and the dataset-level orthogonal validation is not claimed for this individual edge. No native physiological role, partner activity or more-specific binding function is inferred. Supported generic binding is retained as non-core under the standing project instruction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: HuRI reports a BSND interaction with UniProtKB:Q4VAQ0. Reason: The exact source partner UniProtKB:Q4VAQ0 is listed in the curated UniProt/IntAct interaction record (NbExp=3). The inspected HuRI Results describe systematic human-protein Y2H screening, quadruplicate pairwise retesting and sequence confirmation. Together these support retention as a reported experimental binding observation, with deference to curators for this pair's original supplementary record. That target record was not independently inspected; the experiment count is not treated as independent publications, and the dataset-level orthogonal validation is not claimed for this individual edge. No native physiological role, partner activity or more-specific binding function is inferred. Supported generic binding is retained as non-core under the standing project instruction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: HuRI reports a BSND interaction with UniProtKB:Q59EV6. Reason: The exact source partner UniProtKB:Q59EV6 is listed in the curated UniProt/IntAct interaction record (NbExp=3). The inspected HuRI Results describe systematic human-protein Y2H screening, quadruplicate pairwise retesting and sequence confirmation. Together these support retention as a reported experimental binding observation, with deference to curators for this pair's original supplementary record. That target record was not independently inspected; the experiment count is not treated as independent publications, and the dataset-level orthogonal validation is not claimed for this individual edge. No native physiological role, partner activity or more-specific binding function is inferred. Supported generic binding is retained as non-core under the standing project instruction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: HuRI reports a BSND interaction with UniProtKB:Q5VYK3. Reason: The exact source partner UniProtKB:Q5VYK3 is listed in the curated UniProt/IntAct interaction record (NbExp=3). The inspected HuRI Results describe systematic human-protein Y2H screening, quadruplicate pairwise retesting and sequence confirmation. Together these support retention as a reported experimental binding observation, with deference to curators for this pair's original supplementary record. That target record was not independently inspected; the experiment count is not treated as independent publications, and the dataset-level orthogonal validation is not claimed for this individual edge. No native physiological role, partner activity or more-specific binding function is inferred. Supported generic binding is retained as non-core under the standing project instruction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: HuRI reports a BSND interaction with UniProtKB:Q8IWU4. Reason: The exact source partner UniProtKB:Q8IWU4 is listed in the curated UniProt/IntAct interaction record (NbExp=3). The inspected HuRI Results describe systematic human-protein Y2H screening, quadruplicate pairwise retesting and sequence confirmation. Together these support retention as a reported experimental binding observation, with deference to curators for this pair's original supplementary record. That target record was not independently inspected; the experiment count is not treated as independent publications, and the dataset-level orthogonal validation is not claimed for this individual edge. No native physiological role, partner activity or more-specific binding function is inferred. Supported generic binding is retained as non-core under the standing project instruction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: HuRI reports a BSND interaction with UniProtKB:Q8N5M9. Reason: The exact source partner UniProtKB:Q8N5M9 is listed in the curated UniProt/IntAct interaction record (NbExp=3). The inspected HuRI Results describe systematic human-protein Y2H screening, quadruplicate pairwise retesting and sequence confirmation. Together these support retention as a reported experimental binding observation, with deference to curators for this pair's original supplementary record. That target record was not independently inspected; the experiment count is not treated as independent publications, and the dataset-level orthogonal validation is not claimed for this individual edge. No native physiological role, partner activity or more-specific binding function is inferred. Supported generic binding is retained as non-core under the standing project instruction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: HuRI reports a BSND interaction with UniProtKB:Q8N6G5. Reason: The exact source partner UniProtKB:Q8N6G5 is listed in the curated UniProt/IntAct interaction record (NbExp=3). The inspected HuRI Results describe systematic human-protein Y2H screening, quadruplicate pairwise retesting and sequence confirmation. Together these support retention as a reported experimental binding observation, with deference to curators for this pair's original supplementary record. That target record was not independently inspected; the experiment count is not treated as independent publications, and the dataset-level orthogonal validation is not claimed for this individual edge. No native physiological role, partner activity or more-specific binding function is inferred. Supported generic binding is retained as non-core under the standing project instruction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: HuRI reports a BSND interaction with UniProtKB:Q8WY91. Reason: The exact source partner UniProtKB:Q8WY91 is listed in the curated UniProt/IntAct interaction record (NbExp=3). The inspected HuRI Results describe systematic human-protein Y2H screening, quadruplicate pairwise retesting and sequence confirmation. Together these support retention as a reported experimental binding observation, with deference to curators for this pair's original supplementary record. That target record was not independently inspected; the experiment count is not treated as independent publications, and the dataset-level orthogonal validation is not claimed for this individual edge. No native physiological role, partner activity or more-specific binding function is inferred. Supported generic binding is retained as non-core under the standing project instruction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: HuRI reports a BSND interaction with UniProtKB:Q969Z0. Reason: The exact source partner UniProtKB:Q969Z0 is listed in the curated UniProt/IntAct interaction record (NbExp=3). The inspected HuRI Results describe systematic human-protein Y2H screening, quadruplicate pairwise retesting and sequence confirmation. Together these support retention as a reported experimental binding observation, with deference to curators for this pair's original supplementary record. That target record was not independently inspected; the experiment count is not treated as independent publications, and the dataset-level orthogonal validation is not claimed for this individual edge. No native physiological role, partner activity or more-specific binding function is inferred. Supported generic binding is retained as non-core under the standing project instruction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: HuRI reports a BSND interaction with UniProtKB:Q96AL5. Reason: The exact source partner UniProtKB:Q96AL5 is listed in the curated UniProt/IntAct interaction record (NbExp=3). The inspected HuRI Results describe systematic human-protein Y2H screening, quadruplicate pairwise retesting and sequence confirmation. Together these support retention as a reported experimental binding observation, with deference to curators for this pair's original supplementary record. That target record was not independently inspected; the experiment count is not treated as independent publications, and the dataset-level orthogonal validation is not claimed for this individual edge. No native physiological role, partner activity or more-specific binding function is inferred. Supported generic binding is retained as non-core under the standing project instruction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: HuRI reports a BSND interaction with UniProtKB:Q9BQE5. Reason: The exact source partner UniProtKB:Q9BQE5 is listed in the curated UniProt/IntAct interaction record (NbExp=3). The inspected HuRI Results describe systematic human-protein Y2H screening, quadruplicate pairwise retesting and sequence confirmation. Together these support retention as a reported experimental binding observation, with deference to curators for this pair's original supplementary record. That target record was not independently inspected; the experiment count is not treated as independent publications, and the dataset-level orthogonal validation is not claimed for this individual edge. No native physiological role, partner activity or more-specific binding function is inferred. Supported generic binding is retained as non-core under the standing project instruction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: HuRI reports a BSND interaction with UniProtKB:Q9H2L4. Reason: The exact source partner UniProtKB:Q9H2L4 is listed in the curated UniProt/IntAct interaction record (NbExp=3). The inspected HuRI Results describe systematic human-protein Y2H screening, quadruplicate pairwise retesting and sequence confirmation. Together these support retention as a reported experimental binding observation, with deference to curators for this pair's original supplementary record. That target record was not independently inspected; the experiment count is not treated as independent publications, and the dataset-level orthogonal validation is not claimed for this individual edge. No native physiological role, partner activity or more-specific binding function is inferred. Supported generic binding is retained as non-core under the standing project instruction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: HuRI reports a BSND interaction with UniProtKB:Q9HB07. Reason: The exact source partner UniProtKB:Q9HB07 is listed in the curated UniProt/IntAct interaction record (NbExp=3). The inspected HuRI Results describe systematic human-protein Y2H screening, quadruplicate pairwise retesting and sequence confirmation. Together these support retention as a reported experimental binding observation, with deference to curators for this pair's original supplementary record. That target record was not independently inspected; the experiment count is not treated as independent publications, and the dataset-level orthogonal validation is not claimed for this individual edge. No native physiological role, partner activity or more-specific binding function is inferred. Supported generic binding is retained as non-core under the standing project instruction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: HuRI reports a BSND interaction with UniProtKB:Q9NRY7. Reason: The exact source partner UniProtKB:Q9NRY7 is listed in the curated UniProt/IntAct interaction record (NbExp=3). The inspected HuRI Results describe systematic human-protein Y2H screening, quadruplicate pairwise retesting and sequence confirmation. Together these support retention as a reported experimental binding observation, with deference to curators for this pair's original supplementary record. That target record was not independently inspected; the experiment count is not treated as independent publications, and the dataset-level orthogonal validation is not claimed for this individual edge. No native physiological role, partner activity or more-specific binding function is inferred. Supported generic binding is retained as non-core under the standing project instruction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: HuRI reports a BSND interaction with UniProtKB:Q9NZD8. Reason: The exact source partner UniProtKB:Q9NZD8 is listed in the curated UniProt/IntAct interaction record (NbExp=3). The inspected HuRI Results describe systematic human-protein Y2H screening, quadruplicate pairwise retesting and sequence confirmation. Together these support retention as a reported experimental binding observation, with deference to curators for this pair's original supplementary record. That target record was not independently inspected; the experiment count is not treated as independent publications, and the dataset-level orthogonal validation is not claimed for this individual edge. No native physiological role, partner activity or more-specific binding function is inferred. Supported generic binding is retained as non-core under the standing project instruction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: HuRI reports a BSND interaction with UniProtKB:Q9UNK0. Reason: The exact source partner UniProtKB:Q9UNK0 is listed in the curated UniProt/IntAct interaction record (NbExp=3). The inspected HuRI Results describe systematic human-protein Y2H screening, quadruplicate pairwise retesting and sequence confirmation. Together these support retention as a reported experimental binding observation, with deference to curators for this pair's original supplementary record. That target record was not independently inspected; the experiment count is not treated as independent publications, and the dataset-level orthogonal validation is not claimed for this individual edge. No native physiological role, partner activity or more-specific binding function is inferred. Supported generic binding is retained as non-core under the standing project instruction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: HuRI reports a BSND interaction with UniProtKB:Q9Y5P4-2. Reason: The exact source partner UniProtKB:Q9Y5P4-2 is listed in the curated UniProt/IntAct interaction record (NbExp=3). The inspected HuRI Results describe systematic human-protein Y2H screening, quadruplicate pairwise retesting and sequence confirmation. Together these support retention as a reported experimental binding observation, with deference to curators for this pair's original supplementary record. That target record was not independently inspected; the experiment count is not treated as independent publications, and the dataset-level orthogonal validation is not claimed for this individual edge. No native physiological role, partner activity or more-specific binding function is inferred. Supported generic binding is retained as non-core under the standing project instruction. |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The neurodegenerative-disease interaction map reports BSND binding to UniProtKB:O00291. Reason: The exact curated UniProt/IntAct record lists UniProtKB:O00291 (NbExp=3), supporting this reported experimental association. The inspected primary Results describe a map combining retested Y2H observations and curated binary interactions. Retain the generic binding claim with explicit curator deference for the original target record. Its precise construct and individual supplementary result were not independently inspected, and no orthogonal or native-context validation is claimed for this pair. The experiment count is not treated as independent publications, and no partner function is transferred to BSND. |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The neurodegenerative-disease interaction map reports BSND binding to UniProtKB:O76024. Reason: The exact curated UniProt/IntAct record lists UniProtKB:O76024 (NbExp=3), supporting this reported experimental association. The inspected primary Results describe a map combining retested Y2H observations and curated binary interactions. Retain the generic binding claim with explicit curator deference for the original target record. Its precise construct and individual supplementary result were not independently inspected, and no orthogonal or native-context validation is claimed for this pair. The experiment count is not treated as independent publications, and no partner function is transferred to BSND. |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The neurodegenerative-disease interaction map reports BSND binding to UniProtKB:P13473-2. Reason: The exact curated UniProt/IntAct record lists UniProtKB:P13473-2 (NbExp=3), supporting this reported experimental association. The inspected primary Results describe a map combining retested Y2H observations and curated binary interactions. Retain the generic binding claim with explicit curator deference for the original target record. Its precise construct and individual supplementary result were not independently inspected, and no orthogonal or native-context validation is claimed for this pair. The experiment count is not treated as independent publications, and no partner function is transferred to BSND. |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The neurodegenerative-disease interaction map reports BSND binding to UniProtKB:Q9Y371. Reason: The exact curated UniProt/IntAct record lists UniProtKB:Q9Y371 (NbExp=3), supporting this reported experimental association. The inspected primary Results describe a map combining retested Y2H observations and curated binary interactions. Retain the generic binding claim with explicit curator deference for the original target record. Its precise construct and individual supplementary result were not independently inspected, and no orthogonal or native-context validation is claimed for this pair. The experiment count is not treated as independent publications, and no partner function is transferred to BSND. |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The neurodegenerative-disease interaction map reports BSND binding to UniProtKB:Q9Y3C5. Reason: The exact curated UniProt/IntAct record lists UniProtKB:Q9Y3C5 (NbExp=3), supporting this reported experimental association. The inspected primary Results describe a map combining retested Y2H observations and curated binary interactions. Retain the generic binding claim with explicit curator deference for the original target record. Its precise construct and individual supplementary result were not independently inspected, and no orthogonal or native-context validation is claimed for this pair. The experiment count is not treated as independent publications, and no partner function is transferred to BSND. |
| GO:0005886 plasma membrane | IEA GO_REF:0000107 | ACCEPT | Summary: Barttin is a plasma-membrane component of functional ClC-K channel complexes. Reason: The inherited broad location agrees with the curated human record and target-specific channel-expression studies. Human barttin constructs occur at the surface in heterologous epithelial cells; intracellular pools and mutant trafficking defects do not contradict plasma-membrane localization. The ortholog inference is retained without claiming a separate donor-tree audit. |
| GO:0005886 plasma membrane | ISS GO_REF:0000024 | ACCEPT | Summary: Barttin is a plasma-membrane component of functional ClC-K channel complexes. Reason: The inherited broad location agrees with the curated human record and target-specific channel-expression studies. Human barttin constructs occur at the surface in heterologous epithelial cells; intracellular pools and mutant trafficking defects do not contradict plasma-membrane localization. The ortholog inference is retained without claiming a separate donor-tree audit. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-2744228 | ACCEPT | Summary: Reactome places the ClC-K/barttin transport complex at the plasma membrane. Reason: The cited Reactome entry explicitly distinguishes CLCNKA/CLCNKB complexes with BSND from its other chloride-channel examples. Barttin is the associated accessory subunit at the plasma membrane; properties of CLCN1/CLCN2 or their oligomers are not transferred to BSND. |
| GO:0006821 chloride transport | IBA GO_REF:0000033 | ACCEPT | Summary: Barttin contributes to chloride transport through ClC-K-containing channels. Reason: The PAINT assertion is coherent with barttin-dependent channel activation and surface expression. Its ancestral-node provenance is preserved; the number of listed descendant sources is not used to judge support. Participation is through a functional channel subunit, not through transport of barttin as cargo. |
| GO:0006821 chloride transport | IEA GO_REF:0000120 | ACCEPT | Summary: Combined automated evidence assigns barttin a role in chloride transport. Reason: The rat ortholog and Barttin-family mapping agree with directly characterized accessory control of ClC-K chloride currents. Keep the broad transport process; the human protein is not inferred to be an independent chloride pore. |
| GO:0007605 sensory perception of sound | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Barttin-dependent ion transport supports normal auditory physiology. Reason: The normal cached primary abstract of PMID:11734858 directly describes ClC-K/barttin heteromers in basolateral membranes of potassium-secreting inner-ear epithelia and their role in potassium recycling. PMID:19646679 links a hypomorphic BSND allele and selective trafficking impairment to nonsyndromic deafness. Retain the broad sensory process as physiological context for the channel-regulator mechanism, without assigning a separate sensory-receptor activity or claiming the original mouse donor experiment was independently audited. Supporting Evidence: file:human/BSND/BSND-uniprot.txt PMID:11734858 potassium-secreting epithelia of the inner ear. |
| GO:0016323 basolateral plasma membrane | IBA GO_REF:0000033 | ACCEPT | Summary: Barttin is enriched at the basolateral membrane in polarized epithelial channel complexes. Reason: The PAINT record includes target experimental evidence; this is normal ancestral inference, not circularity. The curated human record and source-specific human-construct experiments support basolateral sorting. Retain the original source fields; this does not assert exclusive localization in every tissue or variant. |
| GO:0016323 basolateral plasma membrane | IDA PMID:18776122 Disease-causing dysfunctions of barttin in Bartter syndrome ... | ACCEPT | Summary: Wild-type human barttin constructs sort predominantly basolaterally in polarized MDCK cells. Reason: The original IDA assertion is supported by the independently inspected official primary Results and Figure 5 caption: WT sorts basolaterally, whereas E88X increases apical distribution. Methods identify human barttin constructs in a heterologous epithelial model. The canonical abstract has conflicting apical wording; the primary Results resolve that discrepancy without rewriting the cache. This is not native human-tissue imaging. The in-repository UniProt record independently records basolateral localization with an experimental citation to this paper; its explicit localization statement is included as support. Supporting Evidence: file:human/BSND/BSND-uniprot.txt Note=Mostly sorted at the basolateral membrane. |
| GO:0016323 basolateral plasma membrane | IEA GO_REF:0000120 | ACCEPT | Summary: Barttin is enriched at the basolateral membrane in polarized epithelial channel complexes. Reason: The combined ortholog/subcellular-location mapping agrees with the curated human location. The curated human record and source-specific human-construct experiments support basolateral sorting. Retain the original source fields; this does not assert exclusive localization in every tissue or variant. |
| GO:0016323 basolateral plasma membrane | ISS GO_REF:0000024 | ACCEPT | Summary: Barttin is enriched at the basolateral membrane in polarized epithelial channel complexes. Reason: The ortholog transfer agrees with the experimentally supported human-protein location. The curated human record and source-specific human-construct experiments support basolateral sorting. Retain the original source fields; this does not assert exclusive localization in every tissue or variant. |
| GO:0017081 chloride channel regulator activity | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT assigns the characteristic chloride-channel regulatory activity of barttin. Reason: The term describes binding to and modulation of a chloride channel. Target-specific coexpression, association and disease-variant experiments support this activity. Target inclusion among PAINT descendant sources is legitimate experimental grounding; no unperformed phylogenetic audit is claimed. |
| GO:0017081 chloride channel regulator activity | IDA PMID:12111250 Barttin increases surface expression and changes current pro... | ACCEPT | Summary: Barttin activates ClC-K channels and changes their current properties and membrane abundance. Reason: The cached primary abstract directly reports increased currents, altered biophysical properties, enhanced surface abundance and co-immunoprecipitation with ClC-K. These observations establish the regulator function rather than association alone. Supporting Evidence: |
| GO:0017081 chloride channel regulator activity | IEA GO_REF:0000120 | ACCEPT | Summary: Ortholog-based electronic annotations identify barttin as a chloride-channel regulator. Reason: Mouse and rat evidence transfers are consistent with the directly demonstrated regulatory role of human barttin. The functional conclusion is supported independently of donor count and does not assign another subunit’s pore activity to BSND. |
| GO:0017081 chloride channel regulator activity | IMP PMID:18776122 Disease-causing dysfunctions of barttin in Bartter syndrome ... | ACCEPT | Summary: Disease-associated barttin variants separate channel activation, trafficking and epithelial sorting. Reason: The primary abstract reports heterologous electrophysiology, imaging and biochemical comparisons. Several variants eliminate channel function while allowing surface insertion; other variants affect binding or polarized sorting. The perturbations support a direct accessory regulator role, rather than a generic disease association alone. Supporting Evidence: PMID:18776122 Three missense mutations (R8L, R8W, and G10S) eliminated the function of ClC-K/barttin channels |
| GO:0032991 protein-containing complex | IEA GO_REF:0000107 | MODIFY | Summary: Barttin is an accessory component of ClC-K chloride-channel complexes. Reason: The general complex annotation can be refined to chloride channel complex. Curated subunit information and functional coexpression/co-immunoprecipitation support association with CLCNKA or CLCNKB. The definition concerns the activity of the complex; it does not require every subunit to form the pore. No exact stoichiometry or additional channel partner is inferred. Proposed replacements: chloride channel complex |
| GO:0032991 protein-containing complex | ISS GO_REF:0000024 | MODIFY | Summary: Barttin is an accessory component of ClC-K chloride-channel complexes. Reason: The general complex annotation can be refined to chloride channel complex. Curated subunit information and functional coexpression/co-immunoprecipitation support association with CLCNKA or CLCNKB. The definition concerns the activity of the complex; it does not require every subunit to form the pore. No exact stoichiometry or additional channel partner is inferred. Proposed replacements: chloride channel complex |
| GO:1902476 chloride transmembrane transport | IEA GO_REF:0000108 | ACCEPT | Summary: Barttin supports chloride movement across membranes as a ClC-K channel accessory subunit. Reason: The logical inference from channel activity is appropriate with the preserved contributes_to context of the source channel annotations. Barttin performs regulatory and structural work required for functional channel currents; it is not merely an input or transported substrate. This specific transmembrane process is used once in the core rather than duplicating its chloride-transport ancestor. |
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Download this section (compressed HTML)Q: What physiological contexts and tested constructs underlie the 31 reported HuRI and neurodegeneration-map BSND interactions? These experimental binding observations are retained as non-core with explicit curator deference for the original pair records; their native functions and exact construct-specific effects remain open.
Q: Which human tissues and variants distinguish effects on ClC-K gating, surface abundance and polarized sorting, beyond the currently inspected heterologous epithelial assays?
Q: How do barttin palmitoylation and its separate trafficking and gating domains interact in native renal and inner-ear cells? C54/C56 effects on active channel number and the I12T trafficking phenotype distinguish mechanisms, but do not establish every tissue-specific regulatory step.
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