C2orf15 is a 91-amino-acid protein of unknown function encoded on chromosome 2. Its only recognised feature is a DUF5547 domain (Pfam PF17701); it has no predicted transmembrane segment or signal peptide. The transcript is detected at low levels in many tissues. Proteome-wide studies have recorded yeast two-hybrid interactions with several unrelated proteins and its capture on polyadenylated mRNA in HEK293 cells, and it was reported as up-regulated in invasive pituitary adenomas, but no activity, subcellular location or biological role has been established.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0003723 RNA binding | HDA PMID:22681889 The mRNA-bound proteome and its global occupancy profile on ... | MARK AS OVER ANNOTATED | Summary: RNA binding from a single proteome-wide UV-crosslinking/oligo(dT) mRNA interactome capture in HEK293 cells. Reason: The capture is genuine experimental data, but it is one high-throughput hit for a protein with no known RNA-binding domain, and nearly a third of the proteins in this capture had no prior RNA-binding annotation. Without a validation (e.g. CLIP or binding of recombinant protein) it does not establish RNA binding as a function of C2orf15. Supporting Evidence: PMID:22681889 To our knowledge, nearly one-third were not previously annotated as RNA binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Bare protein binding with DYNLT3 (P51808) from a proteome-scale yeast two-hybrid screen. Reason: Generic protein binding is uninformative and is removed under project policy; removal does not dispute the reported interaction. This is one of five unrelated Y2H partners (DYNLT3, EZR, PKN1, UQCRC1, AGER) that sit in different compartments, none followed up, so no informative molecular function can be inferred. Supporting Evidence: PMID:32296183 yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | REMOVE | Summary: Bare protein binding with EZR (P15311) from a proteome-scale yeast two-hybrid screen. Reason: Generic protein binding is uninformative and is removed under project policy; removal does not dispute the reported interaction. This is one of five unrelated Y2H partners (DYNLT3, EZR, PKN1, UQCRC1, AGER) that sit in different compartments, none followed up, so no informative molecular function can be inferred. Supporting Evidence: PMID:32814053 systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | REMOVE | Summary: Bare protein binding with UQCRC1 (P31930) from a proteome-scale yeast two-hybrid screen. Reason: Generic protein binding is uninformative and is removed under project policy; removal does not dispute the reported interaction. This is one of five unrelated Y2H partners (DYNLT3, EZR, PKN1, UQCRC1, AGER) that sit in different compartments, none followed up, so no informative molecular function can be inferred. Supporting Evidence: PMID:32814053 systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | REMOVE | Summary: Bare protein binding with AGER (Q15109) from a proteome-scale yeast two-hybrid screen. Reason: Generic protein binding is uninformative and is removed under project policy; removal does not dispute the reported interaction. This is one of five unrelated Y2H partners (DYNLT3, EZR, PKN1, UQCRC1, AGER) that sit in different compartments, none followed up, so no informative molecular function can be inferred. Supporting Evidence: PMID:32814053 systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | REMOVE | Summary: Bare protein binding with PKN1 (Q16512) from a proteome-scale yeast two-hybrid screen. Reason: Generic protein binding is uninformative and is removed under project policy; removal does not dispute the reported interaction. This is one of five unrelated Y2H partners (DYNLT3, EZR, PKN1, UQCRC1, AGER) that sit in different compartments, none followed up, so no informative molecular function can be inferred. Supporting Evidence: PMID:32814053 systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins |
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Download this section (compressed HTML)Q: Is C2orf15 N-myristoylated at Gly2 (MGFSLSKS), and does that target it to membranes?
Q: Does C2orf15 bind RNA directly, as its capture on oligo(dT) in HEK293 cells suggests, and if so through which residues?
Experiment: Express C2orf15 wild type and a G2A mutant with metabolic myristate-analogue labelling; compare membrane association by fractionation and localisation by immunofluorescence.
Hypothesis: C2orf15 is a lipid-anchored membrane protein.
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