CAPSL is a calcyphosin-like EF-hand protein with conserved calcium-binding motifs. Human CAPSL is distributed in the cytoplasm and nucleus. Depletion in human retinal microvascular endothelial cells impairs proliferation, migration and tube formation, and endothelial Capsl loss disrupts retinal vascular development in mice. Its molecular connection to MYC signaling remains unresolved; a calcium-dependent regulatory mechanism is plausible but not experimentally established. A mammalian structural preprint identifies CAPSL as a microtubule-inner protein of the ciliary transition zone and reports microtubule stabilization in vitro; the native structural evidence is from bovine cilia.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005509 calcium ion binding | IEA GO_REF:0000002 | ACCEPT | Summary: Conserved EF-hand calcium-binding motifs support calcium ion binding. Reason: The human sequence has four EF-hand domains with explicit calcium-coordinating positions assigned by PROSITE-ProRule. Horse CAPSL is 97.1% identical over all 208 human residues and retains the EF-hand architecture. This is a motif- and conservation-based inference; no direct calcium-binding measurement is asserted. Supporting Evidence: file:human/CAPSL/CAPSL-uniprot.txt /note="EF-hand 1" file:human/CAPSL/CAPSL-uniprot.txt /ligand="Ca(2+)" |
| GO:0005737 cytoplasm | IEA GO_REF:0000044 | ACCEPT | Summary: CAPSL has a supported intracellular cytoplasmic distribution. Reason: The curated localization agrees with CAPSL localization examined in human cells in the retinal angiogenesis study; nuclear distribution is also reported. Cytoplasm is a justified location, without assigning CAPSL to a particular organelle. Supporting Evidence: PMID:39264149 CAPSL is localized in both the cytoplasm and the nucleus of cells |
| GO:0043226 organelle | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Organelle is a broad compatible localization for the reported nuclear pool. Reason: CAPSL has reported nuclear as well as cytoplasmic distribution. The organelle term is therefore compatible but adds little specificity and does not describe its principal biochemical function. The supporting biological observation is the human localization study, not the ARBA assertion that supplied this GOA row. Supporting Evidence: PMID:39264149 CAPSL is localized in both the cytoplasm and the nucleus of cells |
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