CCNB1 encodes cyclin B1, the principal B-type mitotic cyclin (cyclin AB subfamily) and the regulatory subunit of the cyclin-dependent kinase CDK1 (CDC2). Cyclin B1 is not itself an enzyme; it binds CDK1 to form the cyclin B1-CDK1 holoenzyme, historically called M-phase/maturation-promoting factor (MPF), and induces the active kinase conformation while conferring substrate specificity and subcellular targeting on CDK1. Cyclin B1 accumulates through G2 and, once its associated CDK1 is activated (following CDC25-mediated removal of inhibitory Thr14/Tyr15 phosphorylation and reversal of WEE1/PKMYT1 inhibition), the complex drives the G2/M transition and mitotic entry. Cyclin B1 has no single static location: it is predominantly cytoplasmic during interphase, shuttles through the nucleus, and accumulates in the nucleus at prophase after phosphorylation of its cytoplasmic-retention/nuclear-export region; active cyclin B1-CDK1 first appears on centrosomes, and the protein also localizes to spindle microtubules, condensed chromosomes and unattached kinetochores. Through localized CDK1 phosphorylation of substrates (nuclear lamins, condensins, MPS1, separase and many others) it promotes centrosome separation, chromosome condensation, nuclear-envelope breakdown, spindle assembly, correct kinetochore-microtubule attachment and spindle-assembly-checkpoint control. At the metaphase-to-anaphase transition cyclin B1 is polyubiquitinated by the APC/C-CDC20 complex and destroyed by the proteasome; loss of cyclin B1 inactivates CDK1 and drives mitotic exit. A minor fraction of cyclin B1-CDK1 also localizes to the mitochondrial matrix, where it phosphorylates respiratory complex I subunits to support the energetic demands of the G2/M transition.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0000082 G1/S transition of mitotic cell cycle | IBA GO_REF:0000033 | MARK AS OVER ANNOTATED | Summary: IBA propagation of G1/S transition from the ancestral cyclin node. Cyclin B1 is the mitotic B-type cyclin; G1/S commitment is driven by the G1/S cyclins (E/A), not cyclin B1. Reason: The PANTHER cyclin node PTN000019791 groups G1/S cyclins (cyclin E/A/D) with mitotic cyclins (cyclin A/B); the G1/S-transition function is a cyclin E/A activity that has functionally diverged from the B-type cyclins. Cyclin B1 acts at the G2/M transition, not G1/S, so this term over-reaches when propagated to CCNB1. Propagation Review Root cause: PROPAGATION BAD Failure modes: FUNCTIONAL DIVERGENCE Sources checked: PANTHER:PTN000019791 SUPPORTS SOURCE BUT NOT TARGET Ancestral cyclin node spanning G1/S (cyclin E/A/D) and mitotic (cyclin A/B) cyclins; G1/S transition is inherited by the G1/S cyclins, not by the mitotic B-type cyclin CCNB1. |
| GO:0000086 G2/M transition of mitotic cell cycle | NAS PMID:17495531 Cyclin B and cyclin A confer different substrate recognition... | ACCEPT | Summary: Core biological process for cyclin B1. Cyclin B1-CDK1 is the master driver of the G2/M transition. Reason: Cyclin B1 accumulates through G2 and its complex with CDK1 initiates mitosis; this is the defining function of the gene. Supported directly by structural/biochemical work on cyclin B and by the broader literature. Supporting Evidence: PMID:17495531 cyclin B confers M phase-like properties on CDK2 |
| GO:0000922 spindle pole | IDA PMID:18195732 Cyclin B1 is localized to unattached kinetochores and contri... | ACCEPT | Summary: Cyclin B1 is displaced from kinetochores to spindle poles upon microtubule attachment (Chen et al. 2008). Reason: Direct localization evidence: cyclin B1 relocates to spindle poles as kinetochore-microtubule attachment completes. Supporting Evidence: PMID:18195732 Cyclin B1 is displaced from individual kinetochores to the spindle poles by microtubule attachment to the kinetochores |
| GO:0000940 outer kinetochore | IDA PMID:18195732 Cyclin B1 is localized to unattached kinetochores and contri... | ACCEPT | Summary: Cyclin B1 concentrates on the outer plate of the kinetochore during prometaphase (Chen et al. 2008). Reason: Direct experimental localization to the outer kinetochore, where cyclin B1-CDK1 acts on local substrates to promote correct microtubule attachment. Supporting Evidence: PMID:18195732 cyclin B1 is concentrated on the outer plate of the kinetochore during prometaphase |
| GO:0001556 oocyte maturation | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Cyclin B1-CDK1 (MPF) drives oocyte meiotic maturation, a conserved role. Electronically transferred from the rat ortholog. Reason: A genuine conserved cyclin B1 function (MPF in meiotic maturation) but developmental/context-specific and not the core mitotic function; supported here only by automated ortholog transfer, so retained as non-core. |
| GO:0005113 patched binding | IPI PMID:11331587 Patched1 interacts with cyclin B1 to regulate cell cycle pro... | KEEP AS NON CORE | Summary: Cyclin B1 binds patched1 (PTCH1); PTCH1 controls cyclin B1 subcellular localization (Barnes et al. 2001). Reason: A specific, informative molecular-function annotation (binding to a patched receptor) supported by two-hybrid and endogenous co-IP. It is a real but peripheral regulatory interaction, not the core CDK-activation function, so retained as non-core. Supporting Evidence: PMID:11331587 we identified a novel interaction between cyclin B1 and patched1 (ptc1) |
| GO:0005515 protein binding | IPI PMID:10373560 Overproduction of human Myt1 kinase induces a G2 cell cycle ... | REMOVE | Summary: Generic "protein binding" (interactor: PKMYT1 (Myt1)). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported PKMYT1 (Myt1) interaction. |
| GO:0005515 protein binding | IPI PMID:12612082 A novel RING finger protein, human enhancer of invasion 10, ... | REMOVE | Summary: Generic "protein binding" (interactor: CDK1). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDK1 interaction. |
| GO:0005515 protein binding | IPI PMID:12612082 A novel RING finger protein, human enhancer of invasion 10, ... | REMOVE | Summary: Generic "protein binding" (interactor: CCNB1IP1/HEI10). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CCNB1IP1/HEI10 interaction. Supporting Evidence: PMID:12612082 the mitotic cyclin B1 and an E2 ubiquitin-conjugating enzyme were isolated as HEI10-interacting proteins |
| GO:0005515 protein binding | IPI PMID:15790566 Cdk5 activator-binding protein C53 regulates apoptosis induc... | REMOVE | Summary: Generic "protein binding" (interactor: CDK5RAP3 (C53)). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDK5RAP3 (C53) interaction. |
| GO:0005515 protein binding | IPI PMID:17283331 Death-effector domain-containing protein DEDD is an inhibito... | REMOVE | Summary: Generic "protein binding" (interactor: DEDD). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported DEDD interaction. Supporting Evidence: PMID:17283331 DEDD associates with mitotic Cdk1/cyclin B1 complexes via direct binding to cyclin B1 and reduces their function |
| GO:0005515 protein binding | IPI PMID:17283331 Death-effector domain-containing protein DEDD is an inhibito... | REMOVE | Summary: Generic "protein binding" (interactor: CDK1). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDK1 interaction. |
| GO:0005515 protein binding | IPI PMID:18337751 cdc2-cyclin B regulates eEF2 kinase activity in a cell cycle... | REMOVE | Summary: Generic "protein binding" (interactor: CDK1). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDK1 interaction. Supporting Evidence: PMID:18337751 cdc2-cyclin B complexes phosphorylate eEF2K at Ser359 |
| GO:0005515 protein binding | IPI PMID:18408765 CDK1 promotes cell proliferation and survival via phosphoryl... | REMOVE | Summary: Generic "protein binding" (interactor: CDK1). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDK1 interaction. |
| GO:0005515 protein binding | IPI PMID:19502428 Kinase activity-independent regulation of cyclin pathway by ... | REMOVE | Summary: Generic "protein binding" (interactor: PTCH1). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported PTCH1 interaction. |
| GO:0005515 protein binding | IPI PMID:19879842 Regulation of MBK-2/DYRK by CDK-1 and the pseudophosphatases... | REMOVE | Summary: Generic "protein binding" (interactor: CDK1). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDK1 interaction. |
| GO:0005515 protein binding | IPI PMID:20965177 Hip2 interacts with cyclin B1 and promotes its degradation t... | REMOVE | Summary: Generic "protein binding" (interactor: UBE2K (Hip2)). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported UBE2K (Hip2) interaction. Supporting Evidence: PMID:20965177 Hip2 was found to interact with cyclin B1 to promote its degradation through the ubiquitin proteasome pathway |
| GO:0005515 protein binding | IPI PMID:21540187 Inhibitor of cyclin-dependent kinase (CDK) interacting with ... | REMOVE | Summary: Generic "protein binding" (interactor: INCA1). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported INCA1 interaction. |
| GO:0005515 protein binding | IPI PMID:21952639 NIRF constitutes a nodal point in the cell cycle network and... | REMOVE | Summary: Generic "protein binding" (interactor: UHRF2 (NIRF)). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported UHRF2 (NIRF) interaction. |
| GO:0005515 protein binding | IPI PMID:23397142 Analysis of protein-protein interactions in cross-talk pathw... | REMOVE | Summary: Generic "protein binding" (interactor: PKMYT1). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported PKMYT1 interaction. |
| GO:0005515 protein binding | IPI PMID:23455922 Interlaboratory reproducibility of large-scale human protein... | REMOVE | Summary: Generic "protein binding" (interactor: CDK5). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDK5 interaction. |
| GO:0005515 protein binding | IPI PMID:23543736 Ubiquitin C-terminal hydrolase L1 (UCH-L1) acts as a novel p... | REMOVE | Summary: Generic "protein binding" (interactor: CDK1). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDK1 interaction. Supporting Evidence: PMID:23543736 UCH-L1 physically interacts with CDK1, CDK4, and CDK5, enhancing their kinase activity |
| GO:0005515 protein binding | IPI PMID:23602568 The protein interaction landscape of the human CMGC kinase g... | REMOVE | Summary: Generic "protein binding" (interactor: CDK1). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDK1 interaction. |
| GO:0005515 protein binding | IPI PMID:23602568 The protein interaction landscape of the human CMGC kinase g... | REMOVE | Summary: Generic "protein binding" (interactor: CDK5). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDK5 interaction. |
| GO:0005515 protein binding | IPI PMID:23799914 Sulforaphane induced cell cycle arrest in the G2/M phase via... | REMOVE | Summary: Generic "protein binding" (interactor: CDK1). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDK1 interaction. |
| GO:0005515 protein binding | IPI PMID:24358021 Polycomb protein SCML2 regulates the cell cycle by binding a... | REMOVE | Summary: Generic "protein binding" (interactor: CDK1). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDK1 interaction. |
| GO:0005515 protein binding | IPI PMID:25852190 Integrative analysis of kinase networks in TRAIL-induced apo... | REMOVE | Summary: Generic "protein binding" (interactor: CDK1). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDK1 interaction. |
| GO:0005515 protein binding | IPI PMID:25852190 Integrative analysis of kinase networks in TRAIL-induced apo... | REMOVE | Summary: Generic "protein binding" (interactor: CDK5). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDK5 interaction. |
| GO:0005515 protein binding | IPI PMID:26496610 A human interactome in three quantitative dimensions organiz... | REMOVE | Summary: Generic "protein binding" (interactor: CDK1). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDK1 interaction. |
| GO:0005515 protein binding | IPI PMID:26496610 A human interactome in three quantitative dimensions organiz... | REMOVE | Summary: Generic "protein binding" (interactor: CDKN1B (p27)). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDKN1B (p27) interaction. |
| GO:0005515 protein binding | IPI PMID:26496610 A human interactome in three quantitative dimensions organiz... | REMOVE | Summary: Generic "protein binding" (interactor: PKMYT1). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported PKMYT1 interaction. |
| GO:0005515 protein binding | IPI PMID:27626412 Sulforaphane, a Dietary Isothiocyanate, Induces G₂/M Arrest ... | REMOVE | Summary: Generic "protein binding" (interactor: CDK1). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDK1 interaction. |
| GO:0005515 protein binding | IPI PMID:28514442 Architecture of the human interactome defines protein commun... | REMOVE | Summary: Generic "protein binding" (interactor: CDK1). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDK1 interaction. |
| GO:0005515 protein binding | IPI PMID:28514442 Architecture of the human interactome defines protein commun... | REMOVE | Summary: Generic "protein binding" (interactor: CDK5). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDK5 interaction. |
| GO:0005515 protein binding | IPI PMID:32707033 Kinase Interaction Network Expands Functional and Disease Ro... | REMOVE | Summary: Generic "protein binding" (interactor: CDK1). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDK1 interaction. |
| GO:0005515 protein binding | IPI PMID:32707033 Kinase Interaction Network Expands Functional and Disease Ro... | REMOVE | Summary: Generic "protein binding" (interactor: CDK5). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDK5 interaction. |
| GO:0005515 protein binding | IPI PMID:32707033 Kinase Interaction Network Expands Functional and Disease Ro... | REMOVE | Summary: Generic "protein binding" (interactor: PKMYT1). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported PKMYT1 interaction. |
| GO:0005515 protein binding | IPI PMID:33037310 The sequence at Spike S1/S2 site enables cleavage by furin a... | REMOVE | Summary: Generic "protein binding" (interactor: CDK1). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDK1 interaction. |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | REMOVE | Summary: Generic "protein binding" (interactor: CDK1). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDK1 interaction. |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | REMOVE | Summary: Generic "protein binding" (interactor: CDKN1B (p27)). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDKN1B (p27) interaction. |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | REMOVE | Summary: Generic "protein binding" (interactor: CDK5). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDK5 interaction. |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | REMOVE | Summary: Generic "protein binding" (interactor: PKMYT1). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported PKMYT1 interaction. |
| GO:0005515 protein binding | IPI PMID:34290405 Structural basis of human separase regulation by securin and... | REMOVE | Summary: Generic "protein binding" (interactor: CDK1). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDK1 interaction. |
| GO:0005515 protein binding | IPI PMID:34591612 A protein interaction landscape of breast cancer. | REMOVE | Summary: Generic "protein binding" (interactor: CDKN1B (p27)). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDKN1B (p27) interaction. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | REMOVE | Summary: Generic "protein binding" (interactor: CDK1). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDK1 interaction. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | REMOVE | Summary: Generic "protein binding" (interactor: CDK5). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDK5 interaction. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | REMOVE | Summary: Generic "protein binding" (interactor: PKMYT1). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported PKMYT1 interaction. |
| GO:0005515 protein binding | IPI PMID:8756624 Cyclin-binding motifs are essential for the function of p21C... | REMOVE | Summary: Generic "protein binding" (interactor: CDKN1B (p27)). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDKN1B (p27) interaction. |
| GO:0005515 protein binding | IPI PMID:9001210 The human Myt1 kinase preferentially phosphorylates Cdc2 on ... | REMOVE | Summary: Generic "protein binding" (interactor: CDK1). The interaction is not disputed, but GO:0005515 conveys no molecular function. Reason: Per project curation policy, uninformative generic protein_binding is removed. Cyclin B1's informative molecular activity (CDK1 activation and substrate targeting) is captured by GO:0061575 / GO:0016538, and specific interactions such as protein kinase binding (GO:0019901) and patched binding (GO:0005113) are retained separately. Removal does not dispute the reported CDK1 interaction. Supporting Evidence: PMID:9001210 Cdc2 associates with the B-type cyclins |
| GO:0005634 nucleus | IBA GO_REF:0000033 | ACCEPT | Summary: Cyclin B1 accumulates in the nucleus at prophase; nuclear localization is well established. Reason: IBA localization consistent with UniProt (Nucleus) and multiple direct studies. CCNB1 itself is among the WITH/FROM sources, indicating experimental grounding on the target. |
| GO:0005634 nucleus | IDA PMID:11331587 Patched1 interacts with cyclin B1 to regulate cell cycle pro... | ACCEPT | Summary: Nuclear localization of cyclin B1 (Barnes et al. 2001). Reason: Direct immunolocalization; PTCH1/SHH regulate nuclear translocation of cyclin B1. Supporting Evidence: PMID:11331587 allows cyclin B1 to localize to the nucleus |
| GO:0005634 nucleus | IDA PMID:16109376 The bromodomain protein Brd4 is a positive regulatory compon... | ACCEPT | Summary: Nuclear localization of cyclin B1. Reason: Direct-assay nuclear localization annotation, consistent with the established prophase nuclear accumulation of cyclin B1. |
| GO:0005634 nucleus | IDA PMID:20725088 Primate-specific RFPL1 gene controls cell-cycle progression ... | ACCEPT | Summary: Nuclear localization of cyclin B1 (RFPL1 study). Reason: Direct-assay annotation consistent with cyclin B1 nuclear accumulation during the cell cycle. |
| GO:0005634 nucleus | IEA GO_REF:0000120 | ACCEPT | Summary: Nuclear localization by automated methods. Reason: Consistent with experimental nucleus annotations above. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-170044 | ACCEPT | Summary: Nucleoplasm localization from Reactome mitotic pathways. Reason: TAS localization consistent with the nuclear pool of cyclin B1-CDK1 acting on nuclear mitotic substrates. Multiple duplicate Reactome sources; all consistent. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-170070 | ACCEPT | Summary: Nucleoplasm localization from Reactome mitotic pathways. Reason: TAS localization consistent with the nuclear pool of cyclin B1-CDK1 acting on nuclear mitotic substrates. Multiple duplicate Reactome sources; all consistent. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-170072 | ACCEPT | Summary: Nucleoplasm localization from Reactome mitotic pathways. Reason: TAS localization consistent with the nuclear pool of cyclin B1-CDK1 acting on nuclear mitotic substrates. Multiple duplicate Reactome sources; all consistent. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-170076 | ACCEPT | Summary: Nucleoplasm localization from Reactome mitotic pathways. Reason: TAS localization consistent with the nuclear pool of cyclin B1-CDK1 acting on nuclear mitotic substrates. Multiple duplicate Reactome sources; all consistent. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-170131 | ACCEPT | Summary: Nucleoplasm localization from Reactome mitotic pathways. Reason: TAS localization consistent with the nuclear pool of cyclin B1-CDK1 acting on nuclear mitotic substrates. Multiple duplicate Reactome sources; all consistent. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-170153 | ACCEPT | Summary: Nucleoplasm localization from Reactome mitotic pathways. Reason: TAS localization consistent with the nuclear pool of cyclin B1-CDK1 acting on nuclear mitotic substrates. Multiple duplicate Reactome sources; all consistent. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-174122 | ACCEPT | Summary: Nucleoplasm localization from Reactome mitotic pathways. Reason: TAS localization consistent with the nuclear pool of cyclin B1-CDK1 acting on nuclear mitotic substrates. Multiple duplicate Reactome sources; all consistent. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-174132 | ACCEPT | Summary: Nucleoplasm localization from Reactome mitotic pathways. Reason: TAS localization consistent with the nuclear pool of cyclin B1-CDK1 acting on nuclear mitotic substrates. Multiple duplicate Reactome sources; all consistent. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-174251 | ACCEPT | Summary: Nucleoplasm localization from Reactome mitotic pathways. Reason: TAS localization consistent with the nuclear pool of cyclin B1-CDK1 acting on nuclear mitotic substrates. Multiple duplicate Reactome sources; all consistent. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-2245218 | ACCEPT | Summary: Nucleoplasm localization from Reactome mitotic pathways. Reason: TAS localization consistent with the nuclear pool of cyclin B1-CDK1 acting on nuclear mitotic substrates. Multiple duplicate Reactome sources; all consistent. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-2294600 | ACCEPT | Summary: Nucleoplasm localization from Reactome mitotic pathways. Reason: TAS localization consistent with the nuclear pool of cyclin B1-CDK1 acting on nuclear mitotic substrates. Multiple duplicate Reactome sources; all consistent. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-380278 | ACCEPT | Summary: Nucleoplasm localization from Reactome mitotic pathways. Reason: TAS localization consistent with the nuclear pool of cyclin B1-CDK1 acting on nuclear mitotic substrates. Multiple duplicate Reactome sources; all consistent. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-4088024 | ACCEPT | Summary: Nucleoplasm localization from Reactome mitotic pathways. Reason: TAS localization consistent with the nuclear pool of cyclin B1-CDK1 acting on nuclear mitotic substrates. Multiple duplicate Reactome sources; all consistent. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-4088298 | ACCEPT | Summary: Nucleoplasm localization from Reactome mitotic pathways. Reason: TAS localization consistent with the nuclear pool of cyclin B1-CDK1 acting on nuclear mitotic substrates. Multiple duplicate Reactome sources; all consistent. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-5195402 | ACCEPT | Summary: Nucleoplasm localization from Reactome mitotic pathways. Reason: TAS localization consistent with the nuclear pool of cyclin B1-CDK1 acting on nuclear mitotic substrates. Multiple duplicate Reactome sources; all consistent. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-5244669 | ACCEPT | Summary: Nucleoplasm localization from Reactome mitotic pathways. Reason: TAS localization consistent with the nuclear pool of cyclin B1-CDK1 acting on nuclear mitotic substrates. Multiple duplicate Reactome sources; all consistent. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9009282 | ACCEPT | Summary: Nucleoplasm localization from Reactome mitotic pathways. Reason: TAS localization consistent with the nuclear pool of cyclin B1-CDK1 acting on nuclear mitotic substrates. Multiple duplicate Reactome sources; all consistent. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9851092 | ACCEPT | Summary: Nucleoplasm localization from Reactome mitotic pathways. Reason: TAS localization consistent with the nuclear pool of cyclin B1-CDK1 acting on nuclear mitotic substrates. Multiple duplicate Reactome sources; all consistent. |
| GO:0005654 nucleoplasm | TAS Reactome:R-NUL-2434198 | ACCEPT | Summary: Nucleoplasm localization from Reactome mitotic pathways. Reason: TAS localization consistent with the nuclear pool of cyclin B1-CDK1 acting on nuclear mitotic substrates. Multiple duplicate Reactome sources; all consistent. |
| GO:0005737 cytoplasm | IBA GO_REF:0000033 | ACCEPT | Summary: Cyclin B1 is predominantly cytoplasmic during interphase; is_active_in cytoplasm. Reason: IBA localization; cyclin B1-CDK1 is first activated in the cytoplasm/centrosome. CCNB1 appears among its own WITH/FROM sources (experimental grounding on target). |
| GO:0005737 cytoplasm | IDA PMID:11331587 Patched1 interacts with cyclin B1 to regulate cell cycle pro... | ACCEPT | Summary: Cytoplasmic localization of cyclin B1 (Barnes et al. 2001). Reason: Direct localization; PTCH1 sequesters phospho-cyclin B1 in the cytoplasm. |
| GO:0005737 cytoplasm | IDA PMID:17850284 Immunohistochemical analysis of Sonic hedgehog signalling in... | ACCEPT | Summary: Cytoplasmic cyclin B1 by immunohistochemistry in developing human urinary tract. Reason: Direct immunohistochemical localization consistent with the cytoplasmic interphase pool of cyclin B1. Supporting Evidence: PMID:17850284 regulating the subcellular localisation of Cyclin B1 |
| GO:0005737 cytoplasm | IDA PMID:20725088 Primate-specific RFPL1 gene controls cell-cycle progression ... | ACCEPT | Summary: Cytoplasmic localization of cyclin B1 (RFPL1 study). Reason: Direct-assay annotation; cytoplasm-localized RFPL1 prevents cyclin B1 accumulation during interphase. Supporting Evidence: PMID:20725088 cytoplasm-localized hRFPL1 prevented cyclin B1 and Cdc2 accumulation during interphase |
| GO:0005737 cytoplasm | IEA GO_REF:0000120 | ACCEPT | Summary: Cytoplasm localization by automated methods. Reason: Consistent with experimental cytoplasm annotations. |
| GO:0005759 mitochondrial matrix | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: A fraction of cyclin B1-CDK1 colocalizes with the mitochondrial matrix (Wang et al. 2014). Reason: Genuine but non-core moonlighting localization supporting mitochondrial respiration; the IBA node carries CCNB1 in its own WITH/FROM, reflecting the direct human evidence. Retained as non-core. |
| GO:0005759 mitochondrial matrix | IDA PMID:24746669 Cyclin B1/Cdk1 coordinates mitochondrial respiration for cel... | KEEP AS NON CORE | Summary: A fraction of cyclin B1-CDK1 directly localizes to the mitochondrial matrix (Wang et al. 2014). Reason: Directly demonstrated moonlighting localization supporting mitochondrial respiration during G2/M; genuine but peripheral to the core mitotic CDK-activation function, so kept as non-core (consistent with the IBA/IEA mitochondrial-matrix annotations). Supporting Evidence: PMID:24746669 a fraction of cyclin B1/Cdk1 proteins localizes to the matrix of mitochondria and phosphorylates a cluster of mitochondrial proteins |
| GO:0005759 mitochondrial matrix | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Mitochondrial matrix colocalization by automated ortholog transfer. Reason: Non-core moonlighting localization consistent with the direct evidence (PMID:24746669). |
| GO:0005813 centrosome | IDA PMID:12524548 Active cyclin B1-Cdk1 first appears on centrosomes in propha... | ACCEPT | Summary: Active cyclin B1-CDK1 first appears on centrosomes in prophase (Jackman et al. 2003). Reason: Direct evidence that the active complex is first detected at centrosomes, an important site of mitotic initiation. Supporting Evidence: PMID:12524548 cyclin B1 is initially phosphorylated on centrosomes in prophase |
| GO:0005813 centrosome | IEA GO_REF:0000044 | ACCEPT | Summary: Centrosome localization by automated subcellular-location mapping. Reason: Consistent with the direct centrosome annotation (PMID:12524548). |
| GO:0005815 microtubule organizing center | IBA GO_REF:0000033 | ACCEPT | Summary: Microtubule organizing center (centrosome) localization; is_active_in. Reason: MTOC/centrosome is where active cyclin B1-CDK1 first appears; consistent across species (IBA). |
| GO:0005829 cytosol | IDA GO_REF:0000052 | ACCEPT | Summary: Cytosolic localization from immunofluorescence curation. Reason: Consistent with the cytoplasmic interphase pool of cyclin B1. |
| GO:0005829 cytosol | IEA GO_REF:0000117 | ACCEPT | Summary: Cytosol localization by ARBA machine-learning model. Reason: Consistent with experimental cytoplasm/cytosol localization. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-170044 | ACCEPT | Summary: Cytosol localization from Reactome mitotic pathways. Reason: TAS localization consistent with the cytoplasmic pool of cyclin B1-CDK1. Multiple duplicate Reactome sources; all consistent. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-170055 | ACCEPT | Summary: Cytosol localization from Reactome mitotic pathways. Reason: TAS localization consistent with the cytoplasmic pool of cyclin B1-CDK1. Multiple duplicate Reactome sources; all consistent. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-170057 | ACCEPT | Summary: Cytosol localization from Reactome mitotic pathways. Reason: TAS localization consistent with the cytoplasmic pool of cyclin B1-CDK1. Multiple duplicate Reactome sources; all consistent. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-170072 | ACCEPT | Summary: Cytosol localization from Reactome mitotic pathways. Reason: TAS localization consistent with the cytoplasmic pool of cyclin B1-CDK1. Multiple duplicate Reactome sources; all consistent. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-170126 | ACCEPT | Summary: Cytosol localization from Reactome mitotic pathways. Reason: TAS localization consistent with the cytoplasmic pool of cyclin B1-CDK1. Multiple duplicate Reactome sources; all consistent. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-170131 | ACCEPT | Summary: Cytosol localization from Reactome mitotic pathways. Reason: TAS localization consistent with the cytoplasmic pool of cyclin B1-CDK1. Multiple duplicate Reactome sources; all consistent. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-170161 | ACCEPT | Summary: Cytosol localization from Reactome mitotic pathways. Reason: TAS localization consistent with the cytoplasmic pool of cyclin B1-CDK1. Multiple duplicate Reactome sources; all consistent. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-174120 | ACCEPT | Summary: Cytosol localization from Reactome mitotic pathways. Reason: TAS localization consistent with the cytoplasmic pool of cyclin B1-CDK1. Multiple duplicate Reactome sources; all consistent. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-174157 | ACCEPT | Summary: Cytosol localization from Reactome mitotic pathways. Reason: TAS localization consistent with the cytoplasmic pool of cyclin B1-CDK1. Multiple duplicate Reactome sources; all consistent. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-174227 | ACCEPT | Summary: Cytosol localization from Reactome mitotic pathways. Reason: TAS localization consistent with the cytoplasmic pool of cyclin B1-CDK1. Multiple duplicate Reactome sources; all consistent. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-2468287 | ACCEPT | Summary: Cytosol localization from Reactome mitotic pathways. Reason: TAS localization consistent with the cytoplasmic pool of cyclin B1-CDK1. Multiple duplicate Reactome sources; all consistent. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-2468293 | ACCEPT | Summary: Cytosol localization from Reactome mitotic pathways. Reason: TAS localization consistent with the cytoplasmic pool of cyclin B1-CDK1. Multiple duplicate Reactome sources; all consistent. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-2984220 | ACCEPT | Summary: Cytosol localization from Reactome mitotic pathways. Reason: TAS localization consistent with the cytoplasmic pool of cyclin B1-CDK1. Multiple duplicate Reactome sources; all consistent. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-2990882 | ACCEPT | Summary: Cytosol localization from Reactome mitotic pathways. Reason: TAS localization consistent with the cytoplasmic pool of cyclin B1-CDK1. Multiple duplicate Reactome sources; all consistent. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-4086410 | ACCEPT | Summary: Cytosol localization from Reactome mitotic pathways. Reason: TAS localization consistent with the cytoplasmic pool of cyclin B1-CDK1. Multiple duplicate Reactome sources; all consistent. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-6803875 | ACCEPT | Summary: Cytosol localization from Reactome mitotic pathways. Reason: TAS localization consistent with the cytoplasmic pool of cyclin B1-CDK1. Multiple duplicate Reactome sources; all consistent. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9624800 | ACCEPT | Summary: Cytosol localization from Reactome mitotic pathways. Reason: TAS localization consistent with the cytoplasmic pool of cyclin B1-CDK1. Multiple duplicate Reactome sources; all consistent. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9858641 | ACCEPT | Summary: Cytosol localization from Reactome mitotic pathways. Reason: TAS localization consistent with the cytoplasmic pool of cyclin B1-CDK1. Multiple duplicate Reactome sources; all consistent. |
| GO:0005829 cytosol | TAS Reactome:R-NUL-2422970 | ACCEPT | Summary: Cytosol localization from Reactome mitotic pathways. Reason: TAS localization consistent with the cytoplasmic pool of cyclin B1-CDK1. Multiple duplicate Reactome sources; all consistent. |
| GO:0007052 mitotic spindle organization | IMP PMID:18195732 Cyclin B1 is localized to unattached kinetochores and contri... | ACCEPT | Summary: Cyclin B1 depletion impairs spindle-microtubule/kinetochore function and mitotic progression (Chen et al. 2008). Reason: Experimental IMP (siRNA) from a paper whose full text the curator read; kinetochore-localized cyclin B1-CDK1 contributes to correct spindle-microtubule attachment. A downstream mitotic role executed via CDK1 substrate phosphorylation. Supporting Evidence: PMID:18195732 inefficient attachment between kinetochores and microtubules |
| GO:0007080 mitotic metaphase chromosome alignment | IBA GO_REF:0000033 | ACCEPT | Summary: Mitotic metaphase chromosome alignment; IBA and IMP both support a role. Reason: Phylogenetically inferred and experimentally supported (Chen et al. 2008 siRNA causes chromosome alignment defects). CCNB1 is in the IBA WITH/FROM, reflecting target experimental grounding. |
| GO:0007080 mitotic metaphase chromosome alignment | IMP PMID:18195732 Cyclin B1 is localized to unattached kinetochores and contri... | ACCEPT | Summary: Cyclin B1 depletion causes chromosome alignment defects (Chen et al. 2008). Reason: Experimental IMP: kinetochore cyclin B1-CDK1 promotes efficient chromosome alignment. Supporting Evidence: PMID:18195732 chromosome alignment defects |
| GO:0007283 spermatogenesis | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Cyclin B1 has conserved roles in male meiosis; electronically transferred from the rat ortholog. Reason: A genuine but developmental/context-specific role (meiotic MPF) supported only by automated ortholog transfer; retained as non-core. |
| GO:0009410 response to xenobiotic stimulus | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Response-to-xenobiotic transferred from the rat ortholog; reflects abundance/expression change, not participation. Reason: Cyclin B1 levels change in response to many stimuli, but the protein does not execute a step of a xenobiotic-response process; automated ortholog transfer over-annotates a downstream expression readout. |
| GO:0009612 response to mechanical stimulus | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Response-to-mechanical-stimulus (rat ortholog IEA); expression response, not participation. Reason: As for other rat-ortholog response terms: reflects a change in cyclin B1 level, not participation of the protein in the process. |
| GO:0009636 response to toxic substance | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Response-to-toxic-substance (rat ortholog IEA); expression response, not participation. Reason: Downstream abundance readout transferred electronically; cyclin B1 does not perform a step of this process. |
| GO:0010629 negative regulation of gene expression | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Negative regulation of gene expression (rat ortholog IEA); indirect/over-broad. Reason: Cyclin B1 does not directly regulate gene expression; any effect is an indirect consequence of cell-cycle progression. Over-annotation from automated ortholog transfer. |
| GO:0010971 positive regulation of G2/M transition of mitotic cell cycle | IDA PMID:24746669 Cyclin B1/Cdk1 coordinates mitochondrial respiration for cel... | ACCEPT | Summary: Cyclin B1-CDK1 positively regulates the G2/M transition (Wang et al. 2014). Reason: Direct evidence that mitochondrial cyclin B1-CDK1 activity provides bioenergy that promotes G2/M transition; consistent with the core mitotic-entry role. Supporting Evidence: PMID:24746669 efficient bioenergy for G2/M transition |
| GO:0016020 membrane | IEA GO_REF:0000107 | REMOVE | Summary: Membrane localization transferred from the rat ortholog; cyclin B1 is not a membrane protein. Reason: Cyclin B1 has no transmembrane or signal-anchor features and is a soluble cytoplasmic/nuclear/centrosomal protein. This is a spurious automated localization transfer and is demonstrably wrong. |
| GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity | IBA GO_REF:0000033 | ACCEPT | Summary: Core molecular function: cyclin-dependent protein serine/threonine kinase regulator activity. Reason: The defining activity of a cyclin: it modulates (activates and directs) CDK1. IBA at the correct level; CCNB1 is among the WITH/FROM sources. Supporting Evidence: PMID:17495531 The cyclins activate their respective CDKs and confer substrate recognition properties |
| GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity | IEA GO_REF:0000002 | ACCEPT | Summary: CDK regulator activity from InterPro cyclin domain mapping. Reason: Consistent with the core cyclin function; domain-based IEA is appropriate. |
| GO:0019901 protein kinase binding | IEA GO_REF:0000107 | ACCEPT | Summary: Protein kinase (CDK1) binding; automated transfer. Reason: A specific, informative binding term underlying the cyclin-CDK1 interaction; consistent with experimental IPI evidence. |
| GO:0019901 protein kinase binding | IPI PMID:12524548 Active cyclin B1-Cdk1 first appears on centrosomes in propha... | ACCEPT | Summary: Cyclin B1 binds the protein kinase PLK1 (Jackman et al. 2003). Reason: PLK1 phosphorylates cyclin B1 on centrosomes in prophase; protein kinase binding is a specific, informative term (retained rather than removed as generic binding). Supporting Evidence: PMID:12524548 Plk1 phosphorylates cyclin B1 |
| GO:0019901 protein kinase binding | IPI PMID:24746669 Cyclin B1/Cdk1 coordinates mitochondrial respiration for cel... | ACCEPT | Summary: Cyclin B1 binds the protein kinase CDK1 (Wang et al. 2014). Reason: The core CDK1 partnership expressed as a specific protein kinase binding annotation; retained. Supporting Evidence: PMID:24746669 cyclin B1/Cdk1 |
| GO:0031442 positive regulation of mRNA 3'-end processing | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Positive regulation of mRNA 3'-end processing (rat ortholog IEA); indirect. Reason: No direct evidence cyclin B1 participates in mRNA 3'-end processing in human; an indirect/over-propagated ortholog transfer. |
| GO:0042246 tissue regeneration | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Tissue regeneration (rat ortholog IEA); pleiotropic proliferation effect. Reason: Reflects the general requirement for proliferation, not a specific participation of cyclin B1 in a regeneration process. Over-annotation. |
| GO:0044389 ubiquitin-like protein ligase binding | IPI PMID:15749827 A novel UbcH10-binding protein facilitates the ubiquitinylat... | KEEP AS NON CORE | Summary: Cyclin B1 is bound and ubiquitinated in vitro by an UbcH10-binding E3 (H10BH/UBE3D). Reason: A specific binding term (binding an E3 ligase) reflecting cyclin B1's role as a ubiquitination substrate. In vitro evidence for an obscure E3; peripheral to core function, retained as non-core. Supporting Evidence: PMID:15749827 bind cyclin B and ubiquitinylate cyclin B in vitro |
| GO:0044772 mitotic cell cycle phase transition | IEA GO_REF:0000002 | ACCEPT | Summary: Mitotic cell cycle phase transition (InterPro IEA); broad but correct. Reason: Correct broad process for a mitotic cyclin; consistent with the G2/M and mitotic annotations. |
| GO:0045931 positive regulation of mitotic cell cycle | IMP PMID:18195732 Cyclin B1 is localized to unattached kinetochores and contri... | ACCEPT | Summary: Cyclin B1 positively regulates mitotic cell cycle progression (Chen et al. 2008). Reason: Experimental IMP: cyclin B1 depletion delays anaphase onset and slows mitotic progression. Supporting Evidence: PMID:18195732 delays the onset of anaphase |
| GO:0046680 response to DDT | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Response to DDT (rat ortholog IEA); expression response, not participation. Reason: Downstream abundance readout transferred electronically; not participation of cyclin B1 in the process. |
| GO:0048565 digestive tract development | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Digestive tract development (rat ortholog IEA); pleiotropic proliferation effect. Reason: Reflects general proliferation during development, not a specific cyclin B1 role in gut morphogenesis. Over-annotation. |
| GO:0051987 positive regulation of attachment of spindle microtubules to kinetochore | IMP PMID:18195732 Cyclin B1 is localized to unattached kinetochores and contri... | ACCEPT | Summary: Cyclin B1 promotes efficient spindle-microtubule attachment to kinetochores (Chen et al. 2008). Reason: Experimental IMP; kinetochore-localized cyclin B1-CDK1 contributes to correct microtubule attachment. Supporting Evidence: PMID:18195732 contributes to the correct attachment of microtubules to kinetochores |
| GO:0055015 ventricular cardiac muscle cell development | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Ventricular cardiac muscle cell development (rat ortholog IEA); pleiotropic. Reason: Reflects general proliferation, not a specific cyclin B1 participation. Over-annotation from ortholog transfer. |
| GO:0060045 positive regulation of cardiac muscle cell proliferation | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Positive regulation of cardiac muscle cell proliferation (rat ortholog IEA); pleiotropic. Reason: A generic consequence of driving the cell cycle in a particular tissue rather than a distinct cyclin B1 function; over-annotation. |
| GO:0060623 regulation of chromosome condensation | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Cyclin B1-CDK1 phosphorylates condensin subunits, contributing to mitotic chromosome condensation. Reason: A genuine downstream mitotic role (e.g. condensin II NCAPD3 phosphorylation) but electronically transferred here and peripheral to the core CDK-activation function; retained as non-core. |
| GO:0061575 cyclin-dependent protein serine/threonine kinase activator activity | IDA PMID:24746669 Cyclin B1/Cdk1 coordinates mitochondrial respiration for cel... | ACCEPT | Summary: Core molecular function: cyclin-dependent protein serine/threonine kinase ACTIVATOR activity (binds and activates CDK1). Reason: The most specific correct MF for cyclin B1 and the term used in the CCNB1 GO-CAM. Direct evidence that cyclin B1 binds and activates CDK1. Supporting Evidence: PMID:24746669 cyclin B1/Cdk1 |
| GO:0065003 protein-containing complex assembly | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Protein-containing complex assembly (rat ortholog IEA); over-broad. Reason: While cyclin B1 forms the cyclin B1-CDK1 complex, this generic term (any complex assembly) is an over-broad electronic transfer; complex membership is captured by GO:0097125. |
| GO:0071283 cellular response to iron(III) ion | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Cellular response to iron(III) ion (rat ortholog IEA); expression response. Reason: Abundance/expression readout, not participation; over-annotation from automated ortholog transfer. |
| GO:0071398 cellular response to fatty acid | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Cellular response to fatty acid (rat ortholog IEA); expression response. Reason: Abundance/expression readout, not participation; over-annotation. |
| GO:0071456 cellular response to hypoxia | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Cellular response to hypoxia (rat ortholog IEA); expression response. Reason: Cyclin B1 abundance changes with hypoxia-linked cell-cycle arrest, but the protein does not execute a hypoxia-response step; over-annotation. |
| GO:0090266 regulation of mitotic cell cycle spindle assembly checkpoint | IMP PMID:18195732 Cyclin B1 is localized to unattached kinetochores and contri... | ACCEPT | Summary: Cyclin B1-CDK1 regulates the spindle assembly checkpoint (Chen et al. 2008; Hayward et al. 2019). Reason: Experimental IMP: relocalization of cyclin B1 from kinetochores upon attachment contributes to SAC silencing, and cyclin B1-CDK1 creates a checkpoint-permissive state by enabling MPS1 kinetochore localization. Supporting Evidence: PMID:18195732 contribute to inactivation of the spindle assembly checkpoint |
| GO:0097125 cyclin B1-CDK1 complex | IBA GO_REF:0000033 | ACCEPT | Summary: Cyclin B1-CDK1 complex membership; is part_of. IBA at the correct level. Reason: Core complex; CCNB1 is among its own WITH/FROM sources (experimental grounding on the target). |
| GO:0097125 cyclin B1-CDK1 complex | IDA PMID:24746669 Cyclin B1/Cdk1 coordinates mitochondrial respiration for cel... | ACCEPT | Summary: Direct evidence of the cyclin B1-CDK1 complex (Wang et al. 2014). Reason: The cyclin B1-CDK1 holoenzyme (MPF) is directly assayed. Supporting Evidence: PMID:24746669 cyclin B1/Cdk1 |
| GO:0097125 cyclin B1-CDK1 complex | IPI PMID:17495531 Cyclin B and cyclin A confer different substrate recognition... | ACCEPT | Summary: Structural characterization of the cyclin B-CDK complex (Brown et al. 2007). Reason: Crystal structure of phospho-CDK2/cyclin B directly demonstrates the complex and the substrate-recognition role of the cyclin. Supporting Evidence: PMID:17495531 We report the structure of phospho-CDK2/cyclin B |
| GO:1905448 positive regulation of mitochondrial ATP synthesis coupled electron transport | IDA PMID:24746669 Cyclin B1/Cdk1 coordinates mitochondrial respiration for cel... | KEEP AS NON CORE | Summary: Mitochondrial cyclin B1-CDK1 phosphorylates complex I subunits and enhances respiration (Wang et al. 2014). Reason: A genuine, directly demonstrated moonlighting function that supports G2/M energetics, but distinct from and peripheral to the core mitotic CDK-activation role; retained as non-core. Supporting Evidence: PMID:24746669 Cyclin B1/Cdk1-mediated CI phosphorylation enhances CI activity |
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Download this section (compressed HTML)Q: Which mitotic substrates strictly require kinetochore- or centrosome-localized cyclin B1-CDK1 (versus the nuclear or cytoplasmic pool), and how does this spatial partitioning order the events of mitosis?
Q: To what extent is the mitochondrial-matrix cyclin B1-CDK1 pool that phosphorylates complex I a physiologically dedicated function versus a low-level consequence of high mitotic CDK1 activity?
Experiment: Combine analog-sensitive CDK1 with rapid, degron-controlled removal of cyclin B1 and quantitative phosphoproteomics across synchronized mitosis to map the cyclin B1-dependent CDK1 substrate set and separate direct targets from indirect effects.
Experiment: Use endogenously tagged cyclin B1 with live super-resolution imaging plus localization-signal mutants (chromatin arginine anchor, NES/CRS, hydrophobic patch) to dissect how each subcellular pool contributes to distinct mitotic events and to APC/C-mediated destruction timing.
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