CCP110 (CP110) is a coiled-coil centriolar protein that caps the distal ends of centrioles. CP110 binds microtubule plus ends directly, associates with their luminal side, blocks their growth and suppresses depolymerization; together with CPAP it controls the slow, limited elongation of centriolar microtubules. CP110 is recruited early to nascent procentrioles and is required for centriole duplication, including PLK4-driven centriole amplification; its levels are set by CDK phosphorylation, SCF(cyclin F)-mediated ubiquitination and USP33 deubiquitination. With its partner CEP97, and with KIF24 and MPHOSPH9, CP110 forms a cap on the mother centriole that suppresses primary cilium formation in proliferating cells, partly by restraining CEP290; TTBK2 activity at the distal appendages triggers loss of the cap at cilium initiation. In mice CP110 is also required in vivo for subdistal appendage assembly, ciliary vesicle docking and cilium formation, so its role in ciliogenesis is context dependent. CP110 also binds calmodulin and centrin and is required for completion of cytokinesis.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005515 protein binding | IPI PMID:12361598 CP110, a cell cycle-dependent CDK substrate, regulates centr... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:16760425 CP110 cooperates with two calcium-binding proteins to regula... | MODIFY | Summary: Protein binding (IPI) with calmodulin, from the study showing that CP110 binds calmodulin and centrin. Reason: The interaction partner is calmodulin, and the study shows a functional calmodulin-binding region in CP110 whose mutation causes cytokinesis failure; the informative molecular function term is calmodulin binding. Proposed replacements: calmodulin binding Supporting Evidence: PMID:16760425 we report that CP110 interacts with two different Ca2+-binding proteins, calmodulin (CaM) and centrin, in vivo PMID:16760425 Importantly, expression of a CP110 mutant unable to bind CaM also promotes cytokinesis failure and binucleate cell formation. file:human/CCP110/CCP110-deep-research-falcon.md CP110 also binds calmodulin directly through multiple regions; a CaM-binding-defective CP110 mutant still localized to centrosomes but increased binucleate cells, linking this interaction to cytokinesis and genome stability |
| GO:0005515 protein binding | IPI PMID:16760425 CP110 cooperates with two calcium-binding proteins to regula... | MODIFY | Summary: Protein binding (IPI) with calmodulin, from the study showing that CP110 binds calmodulin and centrin. Reason: The interaction partner is calmodulin, and the study shows a functional calmodulin-binding region in CP110 whose mutation causes cytokinesis failure; the informative molecular function term is calmodulin binding. Proposed replacements: calmodulin binding Supporting Evidence: PMID:16760425 we report that CP110 interacts with two different Ca2+-binding proteins, calmodulin (CaM) and centrin, in vivo PMID:16760425 Importantly, expression of a CP110 mutant unable to bind CaM also promotes cytokinesis failure and binucleate cell formation. file:human/CCP110/CCP110-deep-research-falcon.md CP110 also binds calmodulin directly through multiple regions; a CaM-binding-defective CP110 mutant still localized to centrosomes but increased binucleate cells, linking this interaction to cytokinesis and genome stability |
| GO:0005515 protein binding | IPI PMID:16760425 CP110 cooperates with two calcium-binding proteins to regula... | MODIFY | Summary: Protein binding (IPI) with calmodulin, from the study showing that CP110 binds calmodulin and centrin. Reason: The interaction partner is calmodulin, and the study shows a functional calmodulin-binding region in CP110 whose mutation causes cytokinesis failure; the informative molecular function term is calmodulin binding. Proposed replacements: calmodulin binding Supporting Evidence: PMID:16760425 we report that CP110 interacts with two different Ca2+-binding proteins, calmodulin (CaM) and centrin, in vivo PMID:16760425 Importantly, expression of a CP110 mutant unable to bind CaM also promotes cytokinesis failure and binucleate cell formation. file:human/CCP110/CCP110-deep-research-falcon.md CP110 also binds calmodulin directly through multiple regions; a CaM-binding-defective CP110 mutant still localized to centrosomes but increased binucleate cells, linking this interaction to cytokinesis and genome stability |
| GO:0005515 protein binding | IPI PMID:16760425 CP110 cooperates with two calcium-binding proteins to regula... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:17719545 Cep97 and CP110 suppress a cilia assembly program. | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:18694559 CP110 suppresses primary cilia formation through its interac... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:18694559 CP110 suppresses primary cilia formation through its interac... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:19460342 Cep76, a centrosomal protein that specifically restrains cen... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:20596027 SCF(Cyclin F) controls centrosome homeostasis and mitotic fi... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:21620453 Centriolar kinesin Kif24 interacts with CP110 to remodel mic... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:21620453 Centriolar kinesin Kif24 interacts with CP110 to remodel mic... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:21620453 Centriolar kinesin Kif24 interacts with CP110 to remodel mic... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:22441691 Neurl4, a novel daughter centriole protein, prevents formati... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:22441691 Neurl4, a novel daughter centriole protein, prevents formati... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:22441691 Neurl4, a novel daughter centriole protein, prevents formati... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:22441691 Neurl4, a novel daughter centriole protein, prevents formati... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:22632967 Cyclin F-mediated degradation of ribonucleotide reductase M2... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:23486064 USP33 regulates centrosome biogenesis via deubiquitination o... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:23486064 USP33 regulates centrosome biogenesis via deubiquitination o... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:24421332 The CP110-interacting proteins Talpid3 and Cep290 play overl... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:24421332 The CP110-interacting proteins Talpid3 and Cep290 play overl... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:24421332 The CP110-interacting proteins Talpid3 and Cep290 play overl... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:24421332 The CP110-interacting proteins Talpid3 and Cep290 play overl... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:24981860 Human-chromatin-related protein interactions identify a deme... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:26638075 A Dynamic Protein Interaction Landscape of the Human Centros... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:26638075 A Dynamic Protein Interaction Landscape of the Human Centros... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:27173435 An organelle-specific protein landscape identifies novel dis... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:30375385 M-Phase Phosphoprotein 9 regulates ciliogenesis by modulatin... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:35709258 Spatial centrosome proteome of human neural cells uncovers d... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:35709258 Spatial centrosome proteome of human neural cells uncovers d... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:35709258 Spatial centrosome proteome of human neural cells uncovers d... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005515 protein binding | IPI PMID:35709258 Spatial centrosome proteome of human neural cells uncovers d... | REMOVE | Summary: Generic protein binding (IPI) for CCP110. Partners include CEP97, CEP290, KIF24, CEP76, cyclin F, CDK2, Neurl4, USP33, centrin, Talpid3 and MPHOSPH9; their functional significance is captured by the process and location terms. Reason: GO:0005515 protein binding is uninformative about the molecular activity of the gene product. Removal does not dispute that the reported physical interaction occurs; the biologically meaningful consequences of the interaction are captured by the process, location and more specific function terms reviewed elsewhere in this document. |
| GO:0005813 centrosome | IBA GO_REF:0000033 | ACCEPT | Summary: Centrosome localization (IBA). Reason: CP110 is a well-established centrosomal protein located at the distal ends of both mother and daughter centrioles (lost from the mother centriole at ciliogenesis). Supporting Evidence: PMID:12361598 Human CP110 localizes to centrosomes. |
| GO:0005813 centrosome | IDA PMID:12361598 CP110, a cell cycle-dependent CDK substrate, regulates centr... | ACCEPT | Summary: Centrosome localization (IDA). Reason: CP110 is a well-established centrosomal protein located at the distal ends of both mother and daughter centrioles (lost from the mother centriole at ciliogenesis). Supporting Evidence: PMID:12361598 Human CP110 localizes to centrosomes. |
| GO:0005813 centrosome | IDA PMID:16760425 CP110 cooperates with two calcium-binding proteins to regula... | ACCEPT | Summary: Centrosome localization (IDA). Reason: CP110 is a well-established centrosomal protein located at the distal ends of both mother and daughter centrioles (lost from the mother centriole at ciliogenesis). Supporting Evidence: PMID:12361598 Human CP110 localizes to centrosomes. |
| GO:0005813 centrosome | IDA PMID:21399614 Novel asymmetrically localizing components of human centroso... | ACCEPT | Summary: Centrosome localization (IDA). Reason: CP110 is a well-established centrosomal protein located at the distal ends of both mother and daughter centrioles (lost from the mother centriole at ciliogenesis). Supporting Evidence: PMID:12361598 Human CP110 localizes to centrosomes. |
| GO:0005813 centrosome | IDA PMID:22684256 miR-129-3p controls cilia assembly by regulating CP110 and a... | ACCEPT | Summary: Centrosome localization (IDA). Reason: CP110 is a well-established centrosomal protein located at the distal ends of both mother and daughter centrioles (lost from the mother centriole at ciliogenesis). Supporting Evidence: PMID:12361598 Human CP110 localizes to centrosomes. |
| GO:0005813 centrosome | IDA PMID:30375385 M-Phase Phosphoprotein 9 regulates ciliogenesis by modulatin... | ACCEPT | Summary: Centrosome localization (IDA). Reason: CP110 is a well-established centrosomal protein located at the distal ends of both mother and daughter centrioles (lost from the mother centriole at ciliogenesis). Supporting Evidence: PMID:12361598 Human CP110 localizes to centrosomes. |
| GO:0005813 centrosome | IEA GO_REF:0000120 | ACCEPT | Summary: Centrosome localization (IEA). Reason: CP110 is a well-established centrosomal protein located at the distal ends of both mother and daughter centrioles (lost from the mother centriole at ciliogenesis). Supporting Evidence: PMID:12361598 Human CP110 localizes to centrosomes. |
| GO:0005814 centriole | IBA GO_REF:0000033 | ACCEPT | Summary: Centriole localization (IBA). Reason: CP110 associates with the growing distal tips of centrioles and caps the distal end of the mature mother centriole. Supporting Evidence: PMID:17681131 CP110 was recruited early and then associated with the growing distal tips, indicating that centrioles elongate through insertion of alpha-/beta-tubulin underneath a CP110 cap. |
| GO:0005814 centriole | IDA PMID:17681131 Plk4-induced centriole biogenesis in human cells. | ACCEPT | Summary: Centriole localization (IDA). Reason: CP110 associates with the growing distal tips of centrioles and caps the distal end of the mature mother centriole. Supporting Evidence: PMID:17681131 CP110 was recruited early and then associated with the growing distal tips, indicating that centrioles elongate through insertion of alpha-/beta-tubulin underneath a CP110 cap. |
| GO:0005814 centriole | IDA PMID:20596027 SCF(Cyclin F) controls centrosome homeostasis and mitotic fi... | ACCEPT | Summary: Centriole localization (IDA). Reason: CP110 associates with the growing distal tips of centrioles and caps the distal end of the mature mother centriole. Supporting Evidence: PMID:17681131 CP110 was recruited early and then associated with the growing distal tips, indicating that centrioles elongate through insertion of alpha-/beta-tubulin underneath a CP110 cap. |
| GO:0005814 centriole | IDA PMID:30375385 M-Phase Phosphoprotein 9 regulates ciliogenesis by modulatin... | ACCEPT | Summary: Centriole localization (IDA). Reason: CP110 associates with the growing distal tips of centrioles and caps the distal end of the mature mother centriole. Supporting Evidence: PMID:17681131 CP110 was recruited early and then associated with the growing distal tips, indicating that centrioles elongate through insertion of alpha-/beta-tubulin underneath a CP110 cap. |
| GO:0005814 centriole | IEA GO_REF:0000120 | ACCEPT | Summary: Centriole localization (IEA). Reason: CP110 associates with the growing distal tips of centrioles and caps the distal end of the mature mother centriole. Supporting Evidence: PMID:17681131 CP110 was recruited early and then associated with the growing distal tips, indicating that centrioles elongate through insertion of alpha-/beta-tubulin underneath a CP110 cap. |
| GO:0005815 microtubule organizing center | IEA GO_REF:0000107 | ACCEPT | Summary: Microtubule organizing center (IEA, UniProt subcellular location mapping). Reason: Correct but general; centrosome and centriole are more informative and are also annotated. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-2574840 | KEEP AS NON CORE | Summary: Reactome-derived cytosol localization (TAS) for CCP110, from pathway reactions in which CCP110 participates. Reason: Reactome places soluble, non-membrane participants of its reactions in the cytosol compartment. This is not wrong in the broad sense, but the informative location of CCP110 is the distal end of centrioles; cytosol is retained as a non-core, low-information location. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-2574845 | KEEP AS NON CORE | Summary: Reactome-derived cytosol localization (TAS) for CCP110, from pathway reactions in which CCP110 participates. Reason: Reactome places soluble, non-membrane participants of its reactions in the cytosol compartment. This is not wrong in the broad sense, but the informative location of CCP110 is the distal end of centrioles; cytosol is retained as a non-core, low-information location. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-3000310 | KEEP AS NON CORE | Summary: Reactome-derived cytosol localization (TAS) for CCP110, from pathway reactions in which CCP110 participates. Reason: Reactome places soluble, non-membrane participants of its reactions in the cytosol compartment. This is not wrong in the broad sense, but the informative location of CCP110 is the distal end of centrioles; cytosol is retained as a non-core, low-information location. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-3000319 | KEEP AS NON CORE | Summary: Reactome-derived cytosol localization (TAS) for CCP110, from pathway reactions in which CCP110 participates. Reason: Reactome places soluble, non-membrane participants of its reactions in the cytosol compartment. This is not wrong in the broad sense, but the informative location of CCP110 is the distal end of centrioles; cytosol is retained as a non-core, low-information location. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-380272 | KEEP AS NON CORE | Summary: Reactome-derived cytosol localization (TAS) for CCP110, from pathway reactions in which CCP110 participates. Reason: Reactome places soluble, non-membrane participants of its reactions in the cytosol compartment. This is not wrong in the broad sense, but the informative location of CCP110 is the distal end of centrioles; cytosol is retained as a non-core, low-information location. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-380283 | KEEP AS NON CORE | Summary: Reactome-derived cytosol localization (TAS) for CCP110, from pathway reactions in which CCP110 participates. Reason: Reactome places soluble, non-membrane participants of its reactions in the cytosol compartment. This is not wrong in the broad sense, but the informative location of CCP110 is the distal end of centrioles; cytosol is retained as a non-core, low-information location. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-380294 | KEEP AS NON CORE | Summary: Reactome-derived cytosol localization (TAS) for CCP110, from pathway reactions in which CCP110 participates. Reason: Reactome places soluble, non-membrane participants of its reactions in the cytosol compartment. This is not wrong in the broad sense, but the informative location of CCP110 is the distal end of centrioles; cytosol is retained as a non-core, low-information location. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-380303 | KEEP AS NON CORE | Summary: Reactome-derived cytosol localization (TAS) for CCP110, from pathway reactions in which CCP110 participates. Reason: Reactome places soluble, non-membrane participants of its reactions in the cytosol compartment. This is not wrong in the broad sense, but the informative location of CCP110 is the distal end of centrioles; cytosol is retained as a non-core, low-information location. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-380311 | KEEP AS NON CORE | Summary: Reactome-derived cytosol localization (TAS) for CCP110, from pathway reactions in which CCP110 participates. Reason: Reactome places soluble, non-membrane participants of its reactions in the cytosol compartment. This is not wrong in the broad sense, but the informative location of CCP110 is the distal end of centrioles; cytosol is retained as a non-core, low-information location. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-380316 | KEEP AS NON CORE | Summary: Reactome-derived cytosol localization (TAS) for CCP110, from pathway reactions in which CCP110 participates. Reason: Reactome places soluble, non-membrane participants of its reactions in the cytosol compartment. This is not wrong in the broad sense, but the informative location of CCP110 is the distal end of centrioles; cytosol is retained as a non-core, low-information location. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-380455 | KEEP AS NON CORE | Summary: Reactome-derived cytosol localization (TAS) for CCP110, from pathway reactions in which CCP110 participates. Reason: Reactome places soluble, non-membrane participants of its reactions in the cytosol compartment. This is not wrong in the broad sense, but the informative location of CCP110 is the distal end of centrioles; cytosol is retained as a non-core, low-information location. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-380508 | KEEP AS NON CORE | Summary: Reactome-derived cytosol localization (TAS) for CCP110, from pathway reactions in which CCP110 participates. Reason: Reactome places soluble, non-membrane participants of its reactions in the cytosol compartment. This is not wrong in the broad sense, but the informative location of CCP110 is the distal end of centrioles; cytosol is retained as a non-core, low-information location. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5626220 | KEEP AS NON CORE | Summary: Reactome-derived cytosol localization (TAS) for CCP110, from pathway reactions in which CCP110 participates. Reason: Reactome places soluble, non-membrane participants of its reactions in the cytosol compartment. This is not wrong in the broad sense, but the informative location of CCP110 is the distal end of centrioles; cytosol is retained as a non-core, low-information location. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5626223 | KEEP AS NON CORE | Summary: Reactome-derived cytosol localization (TAS) for CCP110, from pathway reactions in which CCP110 participates. Reason: Reactome places soluble, non-membrane participants of its reactions in the cytosol compartment. This is not wrong in the broad sense, but the informative location of CCP110 is the distal end of centrioles; cytosol is retained as a non-core, low-information location. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5626227 | KEEP AS NON CORE | Summary: Reactome-derived cytosol localization (TAS) for CCP110, from pathway reactions in which CCP110 participates. Reason: Reactome places soluble, non-membrane participants of its reactions in the cytosol compartment. This is not wrong in the broad sense, but the informative location of CCP110 is the distal end of centrioles; cytosol is retained as a non-core, low-information location. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5626228 | KEEP AS NON CORE | Summary: Reactome-derived cytosol localization (TAS) for CCP110, from pathway reactions in which CCP110 participates. Reason: Reactome places soluble, non-membrane participants of its reactions in the cytosol compartment. This is not wrong in the broad sense, but the informative location of CCP110 is the distal end of centrioles; cytosol is retained as a non-core, low-information location. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5626699 | KEEP AS NON CORE | Summary: Reactome-derived cytosol localization (TAS) for CCP110, from pathway reactions in which CCP110 participates. Reason: Reactome places soluble, non-membrane participants of its reactions in the cytosol compartment. This is not wrong in the broad sense, but the informative location of CCP110 is the distal end of centrioles; cytosol is retained as a non-core, low-information location. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5696945 | KEEP AS NON CORE | Summary: Reactome-derived cytosol localization (TAS) for CCP110, from pathway reactions in which CCP110 participates. Reason: Reactome places soluble, non-membrane participants of its reactions in the cytosol compartment. This is not wrong in the broad sense, but the informative location of CCP110 is the distal end of centrioles; cytosol is retained as a non-core, low-information location. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8853405 | KEEP AS NON CORE | Summary: Reactome-derived cytosol localization (TAS) for CCP110, from pathway reactions in which CCP110 participates. Reason: Reactome places soluble, non-membrane participants of its reactions in the cytosol compartment. This is not wrong in the broad sense, but the informative location of CCP110 is the distal end of centrioles; cytosol is retained as a non-core, low-information location. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8853419 | KEEP AS NON CORE | Summary: Reactome-derived cytosol localization (TAS) for CCP110, from pathway reactions in which CCP110 participates. Reason: Reactome places soluble, non-membrane participants of its reactions in the cytosol compartment. This is not wrong in the broad sense, but the informative location of CCP110 is the distal end of centrioles; cytosol is retained as a non-core, low-information location. |
| GO:0007099 centriole replication | IBA GO_REF:0000033 | ACCEPT | Summary: Centriole replication (IBA). Reason: CP110 is required for procentriole formation, including PLK4-induced centriole amplification, and associates with nascent procentriole tips. This is one of the two core roles of CP110. Supporting Evidence: PMID:16244668 Plk4 is required--in cooperation with Cdk2, CP110 and Hs-SAS6--for the precise reproduction of centrosomes during the cell cycle PMID:17681131 Plk4, hSas-6, CPAP, Cep135, gamma-tubulin, and CP110 were required at different stages of procentriole formation |
| GO:0007099 centriole replication | IEA GO_REF:0000002 | ACCEPT | Summary: Centriole replication (IEA). Reason: CP110 is required for procentriole formation, including PLK4-induced centriole amplification, and associates with nascent procentriole tips. This is one of the two core roles of CP110. Supporting Evidence: PMID:16244668 Plk4 is required--in cooperation with Cdk2, CP110 and Hs-SAS6--for the precise reproduction of centrosomes during the cell cycle PMID:17681131 Plk4, hSas-6, CPAP, Cep135, gamma-tubulin, and CP110 were required at different stages of procentriole formation |
| GO:0007099 centriole replication | IMP PMID:16244668 The Polo kinase Plk4 functions in centriole duplication. | ACCEPT | Summary: Centriole replication (IMP). Reason: CP110 is required for procentriole formation, including PLK4-induced centriole amplification, and associates with nascent procentriole tips. This is one of the two core roles of CP110. Supporting Evidence: PMID:16244668 Plk4 is required--in cooperation with Cdk2, CP110 and Hs-SAS6--for the precise reproduction of centrosomes during the cell cycle PMID:17681131 Plk4, hSas-6, CPAP, Cep135, gamma-tubulin, and CP110 were required at different stages of procentriole formation |
| GO:0007099 centriole replication | IMP PMID:17681131 Plk4-induced centriole biogenesis in human cells. | ACCEPT | Summary: Centriole replication (IMP). Reason: CP110 is required for procentriole formation, including PLK4-induced centriole amplification, and associates with nascent procentriole tips. This is one of the two core roles of CP110. Supporting Evidence: PMID:16244668 Plk4 is required--in cooperation with Cdk2, CP110 and Hs-SAS6--for the precise reproduction of centrosomes during the cell cycle PMID:17681131 Plk4, hSas-6, CPAP, Cep135, gamma-tubulin, and CP110 were required at different stages of procentriole formation |
| GO:0008017 microtubule binding | IDA PMID:39847124 Centriolar cap proteins CP110 and CPAP control slow elongati... | ACCEPT | Summary: Microtubule binding (IDA) from in vitro reconstitution with purified CP110. Reason: CP110 autonomously binds microtubule plus ends, blocks their growth and inhibits depolymerization; this direct activity underlies its capping function. The more specific microtubule plus-end binding term would also be appropriate. Supporting Evidence: PMID:39847124 We found that whereas CEP97 does not bind to microtubules directly, CP110 autonomously binds microtubule plus ends, blocks their growth, and inhibits depolymerization. |
| GO:0032053 ciliary basal body organization | IBA GO_REF:0000033 | ACCEPT | Summary: Ciliary basal body organization (IBA). Reason: Mouse Cp110 is required for maturation of the mother centriole into a basal body, including subdistal appendage assembly and membrane anchoring; in human cells CP110 removal is part of the mother centriole-to-basal body conversion. The ISS from mouse Q7TSH4 is well founded. Supporting Evidence: PMID:26965371 Our data implicate CP110 in SDA assembly and ciliary vesicle docking, two requisite early steps in cilia formation. |
| GO:0032053 ciliary basal body organization | IEA GO_REF:0000107 | ACCEPT | Summary: Ciliary basal body organization (IEA). Reason: Mouse Cp110 is required for maturation of the mother centriole into a basal body, including subdistal appendage assembly and membrane anchoring; in human cells CP110 removal is part of the mother centriole-to-basal body conversion. The ISS from mouse Q7TSH4 is well founded. Supporting Evidence: PMID:26965371 Our data implicate CP110 in SDA assembly and ciliary vesicle docking, two requisite early steps in cilia formation. |
| GO:0032053 ciliary basal body organization | ISS GO_REF:0000024 | ACCEPT | Summary: Ciliary basal body organization (ISS). Reason: Mouse Cp110 is required for maturation of the mother centriole into a basal body, including subdistal appendage assembly and membrane anchoring; in human cells CP110 removal is part of the mother centriole-to-basal body conversion. The ISS from mouse Q7TSH4 is well founded. Supporting Evidence: PMID:26965371 Our data implicate CP110 in SDA assembly and ciliary vesicle docking, two requisite early steps in cilia formation. |
| GO:0032465 regulation of cytokinesis | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: Regulation of cytokinesis (IEA). Reason: CP110 depletion, or a calmodulin-binding-deficient CP110 mutant, causes late cytokinesis failure and binucleate cells. The evidence is direct, but this is a secondary role relative to centriole duplication/capping. Supporting Evidence: PMID:16760425 its depletion leads to a failure at a late stage of cytokinesis and the formation of binucleate cells |
| GO:0032465 regulation of cytokinesis | IMP PMID:16760425 CP110 cooperates with two calcium-binding proteins to regula... | KEEP AS NON CORE | Summary: Regulation of cytokinesis (IMP). Reason: CP110 depletion, or a calmodulin-binding-deficient CP110 mutant, causes late cytokinesis failure and binucleate cells. The evidence is direct, but this is a secondary role relative to centriole duplication/capping. Supporting Evidence: PMID:16760425 its depletion leads to a failure at a late stage of cytokinesis and the formation of binucleate cells |
| GO:0032991 protein-containing complex | IDA PMID:24421332 The CP110-interacting proteins Talpid3 and Cep290 play overl... | KEEP AS NON CORE | Summary: Protein-containing complex (IDA), CP110-containing complexes with CEP97, KIF24, Talpid3 and CEP290. Reason: Correct but uninformative generic complex term; no specific GO complex term exists for the CP110-CEP97 distal cap. Supporting Evidence: PMID:24421332 We have identified Talpid3/KIAA0586 as a component of a CP110-containing protein complex important for centrosome and cilia function. |
| GO:0045724 positive regulation of cilium assembly | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Positive regulation of cilium assembly (IEA), transferred from mouse Cp110. Reason: Cp110-null mice fail to form cilia in several tissues, showing an in vivo requirement for CP110 in early ciliogenesis (subdistal appendages, ciliary vesicle docking). This coexists with the better-established suppressive role of the cap in cycling cultured cells; retained as a genuine but context-dependent, non-core annotation. Supporting Evidence: PMID:26965371 Here, we demonstrate that CP110 promotes cilia formation in vivo, in contrast to findings in cultured cells. PMID:26965371 We suggest that CP110 has unique context-dependent functions, acting as both a suppressor and a promoter of ciliogenesis. |
| GO:0045724 positive regulation of cilium assembly | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Positive regulation of cilium assembly (ISS), transferred from mouse Cp110. Reason: Cp110-null mice fail to form cilia in several tissues, showing an in vivo requirement for CP110 in early ciliogenesis (subdistal appendages, ciliary vesicle docking). This coexists with the better-established suppressive role of the cap in cycling cultured cells; retained as a genuine but context-dependent, non-core annotation. Supporting Evidence: PMID:26965371 Here, we demonstrate that CP110 promotes cilia formation in vivo, in contrast to findings in cultured cells. PMID:26965371 We suggest that CP110 has unique context-dependent functions, acting as both a suppressor and a promoter of ciliogenesis. |
| GO:0051298 centrosome duplication | IDA PMID:20596027 SCF(Cyclin F) controls centrosome homeostasis and mitotic fi... | ACCEPT | Summary: Centrosome duplication (IDA). Reason: Depletion of CP110 blocks centrosome duplication/amplification, and stabilized CP110 (non-degradable by SCF-cyclin F, or excess due to USP33) drives centrosome over-duplication. Supporting Evidence: PMID:12361598 RNAi-mediated depletion of CP110 indicates that this protein plays an essential role in centrosome duplication. PMID:23486064 excessive CP110 drives centrosome over-duplication and suppresses ciliogenesis, whereas its depletion inhibits centriole amplification |
| GO:0051298 centrosome duplication | IEA GO_REF:0000002 | ACCEPT | Summary: Centrosome duplication (IEA). Reason: Depletion of CP110 blocks centrosome duplication/amplification, and stabilized CP110 (non-degradable by SCF-cyclin F, or excess due to USP33) drives centrosome over-duplication. Supporting Evidence: PMID:12361598 RNAi-mediated depletion of CP110 indicates that this protein plays an essential role in centrosome duplication. PMID:23486064 excessive CP110 drives centrosome over-duplication and suppresses ciliogenesis, whereas its depletion inhibits centriole amplification |
| GO:0051298 centrosome duplication | IMP PMID:12361598 CP110, a cell cycle-dependent CDK substrate, regulates centr... | ACCEPT | Summary: Centrosome duplication (IMP). Reason: Depletion of CP110 blocks centrosome duplication/amplification, and stabilized CP110 (non-degradable by SCF-cyclin F, or excess due to USP33) drives centrosome over-duplication. Supporting Evidence: PMID:12361598 RNAi-mediated depletion of CP110 indicates that this protein plays an essential role in centrosome duplication. PMID:23486064 excessive CP110 drives centrosome over-duplication and suppresses ciliogenesis, whereas its depletion inhibits centriole amplification |
| GO:0051298 centrosome duplication | IMP PMID:16760425 CP110 cooperates with two calcium-binding proteins to regula... | ACCEPT | Summary: Centrosome duplication (IMP). Reason: Depletion of CP110 blocks centrosome duplication/amplification, and stabilized CP110 (non-degradable by SCF-cyclin F, or excess due to USP33) drives centrosome over-duplication. Supporting Evidence: PMID:12361598 RNAi-mediated depletion of CP110 indicates that this protein plays an essential role in centrosome duplication. PMID:23486064 excessive CP110 drives centrosome over-duplication and suppresses ciliogenesis, whereas its depletion inhibits centriole amplification |
| GO:0051298 centrosome duplication | IMP PMID:23486064 USP33 regulates centrosome biogenesis via deubiquitination o... | ACCEPT | Summary: Centrosome duplication (IMP). Reason: Depletion of CP110 blocks centrosome duplication/amplification, and stabilized CP110 (non-degradable by SCF-cyclin F, or excess due to USP33) drives centrosome over-duplication. Supporting Evidence: PMID:12361598 RNAi-mediated depletion of CP110 indicates that this protein plays an essential role in centrosome duplication. PMID:23486064 excessive CP110 drives centrosome over-duplication and suppresses ciliogenesis, whereas its depletion inhibits centriole amplification |
| GO:1902018 negative regulation of cilium assembly | IEA GO_REF:0000107 | ACCEPT | Summary: Negative regulation of cilium assembly (IEA). Reason: Core role: CP110 with CEP97 caps the mother centriole and suppresses ciliogenesis in growing cells; depletion promotes ciliation and enforced expression suppresses ciliation in quiescent cells. Cap removal (downstream of TTBK2 and MPHOSPH9 degradation) is required to initiate ciliogenesis. Supporting Evidence: PMID:17719545 loss of Cep97 or CP110 promotes primary cilia formation in growing cells, and enforced expression of CP110 in quiescent cells suppresses their ability to assemble cilia PMID:30375385 The CP110-CEP97 complex functions as a negative regulator of ciliogenesis |
| GO:1902018 negative regulation of cilium assembly | IMP PMID:17719545 Cep97 and CP110 suppress a cilia assembly program. | ACCEPT | Summary: Negative regulation of cilium assembly (IMP). Reason: Core role: CP110 with CEP97 caps the mother centriole and suppresses ciliogenesis in growing cells; depletion promotes ciliation and enforced expression suppresses ciliation in quiescent cells. Cap removal (downstream of TTBK2 and MPHOSPH9 degradation) is required to initiate ciliogenesis. Supporting Evidence: PMID:17719545 loss of Cep97 or CP110 promotes primary cilia formation in growing cells, and enforced expression of CP110 in quiescent cells suppresses their ability to assemble cilia PMID:30375385 The CP110-CEP97 complex functions as a negative regulator of ciliogenesis |
| GO:1902018 negative regulation of cilium assembly | IMP PMID:30375385 M-Phase Phosphoprotein 9 regulates ciliogenesis by modulatin... | ACCEPT | Summary: Negative regulation of cilium assembly (IMP). Reason: Core role: CP110 with CEP97 caps the mother centriole and suppresses ciliogenesis in growing cells; depletion promotes ciliation and enforced expression suppresses ciliation in quiescent cells. Cap removal (downstream of TTBK2 and MPHOSPH9 degradation) is required to initiate ciliogenesis. Supporting Evidence: PMID:17719545 loss of Cep97 or CP110 promotes primary cilia formation in growing cells, and enforced expression of CP110 in quiescent cells suppresses their ability to assemble cilia PMID:30375385 The CP110-CEP97 complex functions as a negative regulator of ciliogenesis |
| GO:0051010 microtubule plus-end binding | IDA PMID:39847124 Centriolar cap proteins CP110 and CPAP control slow elongati... | NEW | Summary: Proposed: CP110 binds microtubule plus ends directly (in vitro reconstitution and cryo-ET). Reason: The existing IDA microtubule binding annotation from the same study is correct but less specific; the study shows plus-end-specific binding at the luminal side of the plus end, which is the basis of the capping activity. Supporting Evidence: PMID:39847124 We found that CP110 can specifically bind to MT plus ends and block their growth, whereas CEP97 does not interact with MTs directly. PMID:39847124 Cryo-electron tomography revealed that CP110 associates with the luminal side of microtubule plus ends and suppresses protofilament flaring. |
| GO:1903722 regulation of centriole elongation | IDA PMID:39847124 Centriolar cap proteins CP110 and CPAP control slow elongati... | NEW | Summary: Proposed: CP110 controls centriole elongation by capping microtubule plus ends. Reason: CP110 itself performs the step: it binds centriolar microtubule plus ends, blocks their growth and is counteracted by CPAP, and disrupting the CP110-CPAP interaction alters centriole elongation. Centrioles elongate underneath the CP110 cap. Orthologous CCP110 proteins in other species already carry the child term negative regulation of centriole elongation; the parent regulation term is proposed for human because CP110 can both restrain and, with CPAP, permit elongation. Supporting Evidence: PMID:39847124 CP110 directly interacts with CPAP, which acts as a microtubule polymerase that overcomes CP110-induced growth inhibition. PMID:39847124 Disruption of CP110-CPAP interaction in cells inhibits centriole elongation and increases incidence of centriole defects. PMID:17681131 centrioles elongate through insertion of alpha-/beta-tubulin underneath a CP110 cap |
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Download this section (compressed HTML)Q: HPA detects CCP110 only at centriolar satellites (Approved) and not at the centriole/basal body, which contrasts with the extensive literature placing CP110 at distal centriole ends; is this an antibody/epitope accessibility issue or a genuine satellite pool?
Q: How are the suppressive (cultured cycling cells) and promoting (Cp110-null mouse tissues) roles of CP110 in ciliogenesis reconciled mechanistically, e.g. via subdistal appendage assembly versus cap removal timing?
Experiment: Separation-of-function CP110 mutants defective in microtubule plus-end binding but competent for CEP97 binding, tested for centriole length control, cap retention and ciliogenesis suppression in CP110-null RPE1 cells.
Experiment: Validate the HPA centriolar-satellite signal with an independent CP110 antibody and endogenous tagging, co-staining for PCM1 and distal centriole markers.
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