CD247

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0002250 adaptive immune response	IEA GO_REF:0000043	KEEP AS NON CORE	Summary: Broad immune-process context is retained. Reason: The Swiss-Prot keyword mapping agrees with the direct receptor-proximal signaling role established in PMID:7509083. Adaptive immunity is a broad organismal outcome rather than an additional molecular activity.
GO:0002250 adaptive immune response	NAS PMID:29789755 Regulatory mechanisms in T cell receptor signalling.	KEEP AS NON CORE	Summary: Broad immune-process context is retained. Reason: The complete abstract of the T-cell development review supports the TCR-dependent immune context. It is secondary synthesis, not a new CD247 perturbation experiment.
GO:0002250 adaptive immune response	NAS PMID:30976362 T cell receptor signaling for γδT cell development.	KEEP AS NON CORE	Summary: Broad immune-process context is retained. Reason: The available review discusses gamma-delta TCR signaling and its developmental and effector contexts. This supports the broad immune role while leaving subset-specific receptor composition explicit.
GO:0002376 immune system process	IEA GO_REF:0000043	KEEP AS NON CORE	Summary: Broad immune-process context is retained. Reason: The immune-system keyword is correct but much broader than the established TCR and Fc-receptor assembly/signaling mechanisms.
GO:0004888 transmembrane signaling receptor activity	IC PMID:9485181 Assembly of the TCR/CD3 complex: CD3 epsilon/delta and CD3 e...	ACCEPT	Summary: CD247 transduces receptor signals as a membrane-spanning TCR subunit. Reason: The IC explicitly derives from membership in GO:0042105. The current GO-CAM 65c57c3400002666 likewise assigns this activity to CD247; the primary assembly and ITAM-recruitment evidence supports signal transmission. This does not assert autonomous peptide-MHC recognition by the short extracellular CD247 segment. The historical double-TCR stoichiometry proposed in PMID:9485181 is not adopted.
GO:0004888 transmembrane signaling receptor activity	IEA GO_REF:0000002	ACCEPT	Summary: The receptor-signaling family mapping is consistent with CD247 function. Reason: IPR003110 maps the immunoreceptor signaling chain to transmembrane receptor activity. Human complex assembly and phosphorylation-dependent recruitment independently substantiate the signaling role (PMIDs:31461748 and 7509083). The individual InterPro mapping rule was not rederived; ligand specificity belongs to the assembled receptor.
GO:0005515 protein binding	IPI PMID:10704231 Activation of Zap-70 tyrosine kinase due to a structural rea...	MODIFY	Summary: Refine the supported ZAP70 interaction. Reason: The recombinant ZAP70/ITAM study supports engagement of the kinase by phosphorylated receptor motifs; the exact peptide construct was not reread in full Methods. Independent direct zeta-tail/tandem-SH2 binding in PMID:7528772 and the exact reviewed UniProt pair corroborate the target. Refine to the kinase class without assigning kinase catalysis to CD247 or assuming every bound peptide activates ZAP70. Proposed replacements: protein tyrosine kinase binding Supporting Evidence: PMID:10704231 Binding of ZAP-70 to the phosphorylated
GO:0005515 protein binding	IPI PMID:10752619 Alternative modes of binding of proteins with tandem SH2 dom...	MODIFY	Summary: Refine the supported ZAP70 interaction. Reason: The complete abstract reports isothermal titration of tandem-SH2 ZAP70 with its phospholigand, but does not identify the exact receptor peptide. The curated P43403 pair and independently read CD247 phosphopeptide assays in PMID:7528772 support the kinase-class refinement. This is not a claim to have inspected the original target peptide sequence or all controls. Proposed replacements: protein tyrosine kinase binding Supporting Evidence: PMID:10752619 Binding studies using isothermal titration calorimetry
GO:0005515 protein binding	IPI PMID:11891219 A novel Src homology 2 domain-containing molecule, Src-like ...	KEEP AS NON CORE	Summary: Retain the supported SLA2 association at generic specificity. Reason: PMID:11891219 reports activation-dependent SLAP2 association with TCR zeta and ZAP70. SLA2 lacks a kinase domain; its SH2-dependent inhibitory phenotype does not by itself map the CD247 interface. Retain the association at generic specificity under the standing project policy.
GO:0005515 protein binding	IPI PMID:11978774 BY55/CD160 acts as a co-receptor in TCR signal transduction ...	KEEP AS NON CORE	Summary: Retain the supported CD160 association at generic specificity. Reason: The human PBL/T-cell-clone abstract reports CD160 coprecipitation with phosphorylated zeta and LCK. This supports a complex association, not purified binary contact or transfer of CD160 costimulatory/ligand-recognition activity to CD247. Retain generic binding as non-core.
GO:0005515 protein binding	IPI PMID:15832366 Peptide microarrays for the detection of molecular interacti...	MODIFY	Summary: Refine the supported ZAP70 interaction. Reason: The peptide-array study explicitly assays ZAP70 tandem-SH2 recognition of a bis-phosphotyrosine TCR motif. This and the exact P43403 pair support protein tyrosine kinase binding; the microarray does not measure an intrinsic catalytic activity of CD247. Proposed replacements: protein tyrosine kinase binding Supporting Evidence: PMID:15832366 binding to a bis-phosphotyrosine-motif
GO:0005515 protein binding	IPI PMID:16461343 Identification of substrates of human protein-tyrosine phosp...	KEEP AS NON CORE	Summary: Retain the supported LCK association at generic specificity. Reason: Selected primary prose from PMID:16461343 shows LCK phosphorylation of recombinant zeta and LCK-Y505F cotransfection, but the original stable-interface control for this IPI row was not independently recovered. The LCK-zeta association is independently supported by the direct SH2/SH3 experiment in PMID:8626561. Retain generic binding without treating kinase-substrate engagement alone as a mapped docking mechanism.
GO:0005515 protein binding	IPI PMID:16461343 Identification of substrates of human protein-tyrosine phosp...	MODIFY	Summary: Refine the supported PTPN22 interaction. Reason: Purified phosphorylated zeta interacts with the PTPN22 D195A/C227S substrate trap, with nonphosphorylated/wild-type controls in the selected primary Results; cellular co-IP used cotransfected 293T proteins. Protein phosphatase binding captures this substrate interaction. CD247 phosphatase-regulator activity is not inferred. Proposed replacements: protein phosphatase binding Supporting Evidence: PMID:16461343 PTPN22-D195A/C227S interacted directly
GO:0005515 protein binding	IPI PMID:18320063 T cell receptor engagement triggers its CD3epsilon and CD3ze...	KEEP AS NON CORE	Summary: Retain the supported NCK1 association at generic specificity. Reason: The primary NCK SH3 interface is mapped to the proline-rich region of CD3epsilon, not CD247. Zeta constructs and complex-level readouts plus the reviewed NCK1 edge support TCR-complex coassociation. Retain generic binding as non-core, without transferring the epsilon SH3-binding mechanism to zeta.
GO:0005515 protein binding	IPI PMID:21957439 The transmembrane adaptor protein SIT inhibits TCR-mediated ...	MODIFY	Summary: Refine the supported ZAP70 interaction. Reason: The actual Jurkat co-IP controls compare SIT RNAi with control and show enhanced ZAP70 recovery with CD3zeta after stimulation, with zeta loading controls. Refine the exact kinase pair while distinguishing cellular complex association from purified contact and from SIT regulatory activity. Proposed replacements: protein tyrosine kinase binding Supporting Evidence: PMID:21957439 analysis of immunoprecipitated TCRζ showed that the association with ZAP-70 was also increased
GO:0005515 protein binding	IPI PMID:22912825 The adaptor protein SAP directly associates with CD3ζ chain ...	MODIFY	Summary: Refine the supported SH2D1A interaction. Reason: Jurkat GST/GST-SAP far-Western and co-IP controls establish direct association; HeLa zeta ITAM tyrosine mutants localize binding to the first ITAM. SAP is essentially an SH2-domain protein. Refine to binding of the partner SH2 domain, not to SAP adaptor activity or CD247 phosphotyrosine recognition. Proposed replacements: SH2 domain binding Supporting Evidence: PMID:22912825 SAP is a direct interactor of the CD3ζ chain
GO:0005515 protein binding	IPI PMID:22922463 End-binding protein 1 controls signal propagation from the T...	UNDECIDED	Summary: The exact mouse Ccr7 partner assertion requires source-specific adjudication. Reason: The immutable P47774 partner is mouse Ccr7, and reviewed UniProt positively records the same Xeno edge. Newly inspected primary Results, Methods and Fig.1 establish CD3 zeta–EB1 assays but do not expose this particular Ccr7 construct or assay. The matching database edge may share the original IntAct provenance, so it is not independent recovery of that experiment. Preserve the positive curated record and source fields while leaving the exact cross-species assertion unresolved; do not substitute EB1 or claim the interaction is false.
GO:0005515 protein binding	IPI PMID:23858057 CD81 controls sustained T cell activation signaling and defi...	KEEP AS NON CORE	Summary: The source directly tests CD3 zeta–CD81 proximity during immune-synapse formation. Reason: The zeta-specific construct and control details come from selected external original Methods, Results and the Figure 4C caption at https://pmc.ncbi.nlm.nih.gov/articles/PMC3753866/, not from the partial canonical HTML cache. Those passages identify CD3 zeta-mEGFP and CD81-mCherry in human J77 cells and phasorFLIM-FRET comparisons with donor-only, ICAM3 and nonspecific-contact controls. They support increasing zeta-CD81 proximity during immune-synapse maturation. The canonical cache independently supplies the abstract and portions of the introduction/discussion; its separate CD3-delta yeast-two-hybrid account is not substituted for the externally inspected zeta experiment. Retain the qualitative association without inferring a purified binary interface or catalytic function; no complete-paper, supplement or figure-pixel inspection is claimed.
GO:0005515 protein binding	IPI PMID:24502978 β-Arrestin-1 mediates the TCR-triggered re-routing of distal...	MODIFY	Summary: Refine the supported ARRB1 interaction. Reason: The available primary Introduction/Discussion identifies beta-arrestin-1 as a direct ligand of nonphosphorylated TCR ITAMs, and reviewed UniProt corroborates the exact P49407 pair. Refine to arrestin-family binding. The partial HTML cache lacks the complete Results/Methods and individual zeta peptide controls; phosphorylation of ARRB1 is catalyzed by PKC, not CD247. Proposed replacements: arrestin family protein binding Supporting Evidence: PMID:24502978 a novel direct ligand of the TCR
GO:0005515 protein binding	IPI PMID:2532305 Co-association of CD3 zeta with a receptor (CD16) for IgG Fc...	KEEP AS NON CORE	Summary: Retain the supported FCGR3A association at generic specificity. Reason: Human NK-cell coassociation and COS7 cotransfection support CD16/CD247 complex assembly. Retain this receptor-complex interaction as generic non-core binding; IgG recognition is not assigned to CD247 from the FCGR3A partner.
GO:0005515 protein binding	IPI PMID:26783323 A novel human autoimmune syndrome caused by combined hypomor...	MODIFY	Summary: Refine the supported ZAP70 interaction. Reason: The selected 2016 primary Results compare WT ZAP70 with R192W and SH2-defective controls in 293T cells with matched input and FLAG-zeta recovery. The zeta construct uses a CD2 transmembrane segment for expression without native TCR. Refine kinase binding without describing the engineered assay as a native patient-T-cell interaction experiment. Proposed replacements: protein tyrosine kinase binding Supporting Evidence: PMID:26783323 both WT and R360P were comparably coimmunoprecipitated with the FLAG-ζ chain
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	UNDECIDED	Summary: The exact ATXN2 isoform-5 interaction remains source-specific uncertainty. Reason: PMID:32814053 is the Haenig neurodegeneration interactome, not HuRI. Reviewed UniProt positively lists Q99700-5 with three experiments, but the original pair table and isoform-specific construct/assay were not independently recovered. That curated record is preserved without describing it as an independently inspected primary experiment. Leave the exact source assertion unresolved; this does not reject the reported interaction or imply a neuronal molecular function for CD247.
GO:0005515 protein binding	IPI PMID:7509083 Sequential interactions of the TCR with two distinct cytopla...	MODIFY	Summary: Refine the supported ZAP70 interaction. Reason: The source reports sequential LCK-dependent ITAM phosphorylation and recruitment of ZAP70 through both SH2 domains. Selected author-reproduction Fig.1 and Methods identify human Jurkat and LCK-deficient J.CaM1 controls. Refine to protein tyrosine kinase binding; phosphorylation chemistry belongs to LCK/ZAP70, while CD247 supplies the docking motifs. Proposed replacements: protein tyrosine kinase binding Supporting Evidence: PMID:7509083 recruitment of a second cytoplasmic PTK, ZAP-70
GO:0005515 protein binding	IPI PMID:7528772 ZAP-70 binding specificity to T cell receptor tyrosine-based...	MODIFY	Summary: Refine the supported ZAP70 interaction. Reason: The detailed abstract describes purified ZAP70 single/tandem SH2 domains and zeta cytoplasmic/TAM peptides, with nonphosphorylated and singly phosphorylated controls. Both phosphotyrosines support high-affinity tandem-SH2 binding. Kinase-class binding is the informative refinement; neither intrinsic CD247 catalysis nor a requirement for every ITAM to have identical affinity follows. Proposed replacements: protein tyrosine kinase binding Supporting Evidence: PMID:7528772 bind phosphorylated, but not nonphosphorylated, TCR zeta cyt
GO:0005515 protein binding	IPI PMID:8626561 Association between mitogen-activated protein kinase and the...	MODIFY	Summary: Refine the supported LCK interaction. Reason: GST-LCK SH2/SH3 assays directly bind phosphorylated zeta; both domains are required, with PLCgamma1 and FYN negative controls. Refine to protein tyrosine kinase binding without claiming an exclusively SH2 interface or transferring the separate LCK-MAPK interaction to CD247. Proposed replacements: protein tyrosine kinase binding Supporting Evidence: PMID:8626561 required both the SH3 and SH2 domains
GO:0005515 protein binding	IPI PMID:8648092 Human and mouse killer-cell inhibitory receptors recruit PTP...	MODIFY	Summary: Independent direct evidence supports a protein tyrosine kinase binding refinement for ZAP70. Reason: The source partner P43403 is ZAP70. The original KIR-focused PMID:8648092 target control remains uninspected, but independent PMID:7528772 directly tests phosphorylated zeta peptides against tandem ZAP70 SH2 domains, with nonphosphorylated and singly phosphorylated controls; PMID:7509083 separately supports recruitment. These same-edge primary data justify the kinase-partner class without claiming new access to the original KIR assay or assigning kinase catalysis to CD247. Proposed replacements: protein tyrosine kinase binding Supporting Evidence: PMID:7528772 ZAP-70 SH2 domains bind phosphorylated, but not nonphosphorylated, TCR zeta cyt.
GO:0005515 protein binding	IPI PMID:8901551 Mechanism of activation for Zap-70 catalytic activity.	MODIFY	Summary: Refine the supported ZAP70 interaction. Reason: Purified ZAP70 interacts with phosphorylated GST-zeta constructs. Dimeric GST presentation increased catalytic output, whereas free phosphorylated zeta/ITAM fragments did not. Refine the kinase-binding relation and preserve the presentation-dependent activation limit; CD247 is not itself a kinase. Proposed replacements: protein tyrosine kinase binding Supporting Evidence: PMID:8901551 Zap-70 is activated upon binding a dimeric form
GO:0005515 protein binding	IPI PMID:9185620 Interaction between the SH2 domains of ZAP-70 and the tyrosi...	MODIFY	Summary: Refine the supported ZAP70 interaction. Reason: The source uses Biacore and WT/single-site ZAP70 SH2 mutants with zeta ITAM1. This directly supports the kinase-binding relation through the partner SH2 domains. Protein conformational changes were measured in the kinase-domain construct, not an intrinsic enzymatic activity of CD247. Proposed replacements: protein tyrosine kinase binding Supporting Evidence: PMID:9185620 binding between TCR zeta ITAM1 and the ZAP-70 SH2
GO:0005737 cytoplasm	IDA PMID:11390434 The transmembrane adaptor protein TRIM regulates T cell rece...	KEEP AS NON CORE	Summary: An intracellular CD247 pool is experimentally observed. Reason: Actual human Jurkat confocal Results in PMID:11390434 distinguish plasma-membrane and intracellular zeta pools. Cytoplasm includes intracellular structures and is not equivalent to freely soluble cytosol. Retain this trafficking context outside the membrane-signaling core; no organelle identity is inferred from the broad term.
GO:0005794 Golgi apparatus	IEA GO_REF:0000107	UNDECIDED	Summary: The specific Golgi localization transfer remains unresolved. Reason: The source is an electronic Ensembl Compara transfer from mouse P24161. The human intracellular pool in PMID:11390434 does not by itself identify Golgi, and the exact mouse donor localization assay was not inspected. General passage of a membrane protein through the secretory pathway is not sufficient evidence for this specific compartment assertion.
GO:0005886 plasma membrane	IDA PMID:11390434 The transmembrane adaptor protein TRIM regulates T cell rece...	ACCEPT	Summary: Human CD247 is present at the plasma membrane. Reason: Human Jurkat imaging in the actual Results of PMID:11390434 detects a plasma-membrane zeta pool as well as intracellular protein; TRIM overexpression shifts its distribution. The original is_active_in qualifier is retained separately, without claiming exclusive plasma-membrane residence. Supporting Evidence: PMID:11390434 only half of endogenous ζ localizes at the plasma membrane
GO:0005886 plasma membrane	IDA PMID:11390434 The transmembrane adaptor protein TRIM regulates T cell rece...	ACCEPT	Summary: Human CD247 is present at the plasma membrane. Reason: Human Jurkat imaging in the actual Results of PMID:11390434 detects a plasma-membrane zeta pool as well as intracellular protein; TRIM overexpression shifts its distribution. The original located_in qualifier is retained separately, without claiming exclusive plasma-membrane residence. Supporting Evidence: PMID:11390434 only half of endogenous ζ localizes at the plasma membrane
GO:0005886 plasma membrane	IDA PMID:1390434 Five novel antigens illustrate shared phenotype between mous...	ACCEPT	Summary: The curated plasma-membrane localization is independently supported. Reason: The identifier, title and complete abstract of PMID:1390434 have been checked. The abstract describes mouse thymic-cell antigens, but it does not expose the source-specific human CD247 localization assay. Publisher access attempts did not recover the original full Methods or Results. This is an unresolved evidence-to-annotation link, not positive evidence that the full paper contains no relevant experiment. Preserve the original IDA identifier and request the original curator/reference mapping; neither MISCITED nor a replacement PMID is established from the available abstract alone. Independent human experiments in PMID:2532305 and PMID:11390434 support plasma-membrane localization, so the annotation remains ACCEPT without relying on the unresolved citation. Database display dates are not used to adjudicate the original experiment.
GO:0005886 plasma membrane	IDA PMID:2532305 Co-association of CD3 zeta with a receptor (CD16) for IgG Fc...	ACCEPT	Summary: Human NK-cell CD247 is membrane-associated with CD16. Reason: PMID:2532305 explicitly describes a zeta homodimer associated with CD16 on human NK-cell membranes, plus COS7 cotransfection. The distinct is_active_in source assertion is preserved; IgG binding is not attributed to zeta.
GO:0005886 plasma membrane	IDA PMID:2532305 Co-association of CD3 zeta with a receptor (CD16) for IgG Fc...	ACCEPT	Summary: Human NK-cell CD247 is membrane-associated with CD16. Reason: PMID:2532305 explicitly describes a zeta homodimer associated with CD16 on human NK-cell membranes, plus COS7 cotransfection. The distinct located_in source assertion is preserved; IgG binding is not attributed to zeta.
GO:0005886 plasma membrane	IDA PMID:31461748 Structural basis of assembly of the human T cell receptor-CD...	ACCEPT	Summary: The human TCR-CD3 structure places CD247 in the membrane-spanning complex. Reason: PMID:31461748 resolves the human transmembrane helices and zeta-zeta module. The complete official 2021 correction (https://doi.org/10.1038/s41586-021-03245-4) adds omitted subunit sequences and construct information; it does not state a reversal of this assembly/localization result. The cache is abstract-only and external access covered selected publisher captions, not full Methods. Supporting Evidence: PMID:31461748 all the transmembrane helices of TCR-CD3
GO:0005886 plasma membrane	IEA GO_REF:0000120	ACCEPT	Summary: Combined electronic plasma-membrane inference agrees with direct human evidence. Reason: The immutable source combines the mouse ortholog and Swiss-Prot subcellular-location mapping. Human membrane localization is directly supported by PMIDs:2532305 and 11390434; the individual combined-method contribution was not reconstructed.
GO:0005886 plasma membrane	ISS GO_REF:0000114	ACCEPT	Summary: The homologous receptor-complex localization transfer is sound. Reason: The CPX-6482 ISS assertion is consistent with the human TCR-CD3 structure and membrane localization. The complete Complex Portal transfer record was not independently inspected; direct human evidence supports the same compartment without equating complex membership with catalytic activity.
GO:0005886 plasma membrane	NAS PMID:30976362 T cell receptor signaling for γδT cell development.	ACCEPT	Summary: The gamma-delta TCR review supports membrane receptor context. Reason: The available review describes membrane TCR signaling. Direct human CD247 membrane evidence independently establishes the location; this NAS is retained as secondary support rather than treated as a new localization assay. Supporting Evidence: PMID:30976362 express γδTCR/CD3 complex on the plasma membrane
GO:0005886 plasma membrane	TAS PMID:3785426 A new subunit of the human T-cell antigen receptor complex.	ACCEPT	Summary: The original human zeta-chain identification supports receptor-associated membrane localization. Reason: The complete abstract reports identification of human zeta through reactivity with antisera against mouse zeta. Its TCR association and independent later human complex evidence support the plasma-membrane location; no claim to have read the full 1986 paper is made.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-164943	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Nef mediated activation of the T-cell receptor. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-198955	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes TCR complex interacts with peptide antigen-presenting MHC Class I. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-202165	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Phosphorylation of ITAM motifs in CD3 complexes. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-202168	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Phosphorylation of ZAP-70 by Lck. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-202174	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Activation of ZAP-70. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-202214	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Dephosphorylation of Lck-pY505 by CD45. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-202216	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Phosphorylation of SLP-76. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-202233	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Inactivation of Lck by Csk. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-202245	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Phosphorylation of TBSMs in LAT. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-202248	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Phosphorylation of PLC-gamma1. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-202291	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Activation of Lck. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-202307	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Change of PKC theta conformation. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-202344	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Recruitment of ZAP-70 to phosphorylated ITAMs. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-2029268	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Phosphorylation and activation of PLCG. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-2029270	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Recruitment of PLCgamma to membrane. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-2029271	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes PI3K phosphorylates PIP2 to PIP3. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-2029272	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Release of PLCG from FCGR. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-2029273	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes PI3K binds to p-6Y-SYK. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-2029451	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Activation of RAC1 by VAV. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-2029452	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Recruitment of SYK to phosphorylated ITAMs. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-2029453	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Phosphorylation of VAV. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-2029457	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Cross-linking of FCGRIIIA with IgG-coated Ag. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-2029458	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Recruitment of VAV1 to p-6Y-SYK. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-2029459	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Sequestering and phosphorylation Fc gamma receptors in the lipid rafts. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-2029467	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Activation of RAC1 by CRKII:DOCK180:ELMO. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-2029476	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Role of myosins in phagosome formation. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-2197697	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Recruitment of CRKII:DOCK180:ELMO complex to FCGR. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-389758	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Dephosphorylation of CD3-zeta by PD-1 bound phosphatases. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-5685600	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes viral HA binds NCRI. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-5685602	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes NCR3LG1 binds NCR3. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-6793275	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes CMVPP65 binds NCR3. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-8850326	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes TCR binds self-lipid-based antigen via CD1. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-8850356	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes TCR binds microbial lipid-based antigen via CD1. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-8852200	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Inactivation of LCK by PTPN22. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-8855381	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes PTPN22 dephosphorylates ZAP70. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-9664261	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Src phosphorylate SYK in IgG:Leishmania surface:p-FCGR3A:SYK. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-9664268	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Opsonized leishmania amastigote binds FCGR3. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-9664270	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Recruitment of PLCgamma to membrane due to FCGR3A effect. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-9664271	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Release of PLCG from FCGR3A. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-9664273	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes SYK binds IgG:Lma antigens:FCGR3A:p-CD3 dimers. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-9664275	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Src phosphorylates CD3 dimers in IgG:Lma antigens:FCGR3A:CD3 dimers. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-9664278	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes Phosphorylation and activation of PLCG due to FCGR3A effect. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-9664406	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes IgG:Lma antigens binds FCGR3A:CD3 dimers. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-9666425	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes p-6Y-SYK phosphorylates VAV1,2,3. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-9666426	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes CRKII:DOCK180:ELMO binds FCGR3A. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-9666428	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes DOCK180 exchanges GTP for GDP on RAC1:GDP. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-9666430	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes p-VAV1,2,3 exchanges GTP for GDP on RAC1:GDP. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-9666435	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes VAV1,2,3:PI(3,4,5)P3 binds p-6Y-SYK. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0005886 plasma membrane	TAS Reactome:R-HSA-9666458	ACCEPT	Summary: Retain plasma-membrane localization in the curated receptor pathway. Reason: The complete cached Reactome entry describes IgG:Leishmania surface:FCGR3A translocates from plasma membrane to the parasitophorous vacuole. This TAS asserts CD247 location, not that CD247 catalyzes every reaction named by the pathway. Direct human zeta localization and receptor-complex structures independently substantiate the compartment. The cached narrative does not enumerate every component-level link, so no uninspected detailed event membership is claimed.
GO:0007166 cell surface receptor signaling pathway	IC PMID:9485181 Assembly of the TCR/CD3 complex: CD3 epsilon/delta and CD3 e...	ACCEPT	Summary: CD247 directly participates in cell-surface receptor signal transmission. Reason: The IC derives from TCR complex membership. CD247 contributes its transmembrane assembly interface and cytoplasmic docking motifs to signaling; this is molecular participation, not merely a downstream knockout phenotype. The historical stoichiometric model in PMID:9485181 is not adopted.
GO:0007166 cell surface receptor signaling pathway	IEA GO_REF:0000002	ACCEPT	Summary: The electronic receptor-signaling pathway assignment is biologically supported. Reason: The InterPro family mapping agrees with direct receptor assembly and ITAM-mediated recruitment. It does not imply CD247 supplies the antigen-binding specificity or kinase chemistry.
GO:0016020 membrane	IEA GO_REF:0000002	KEEP AS NON CORE	Summary: The broad membrane parent is correct but nonspecific. Reason: CD247 is a single-pass receptor-associated membrane protein, with plasma membrane independently established. Retain this InterPro-derived parent outside the more informative location synthesis.
GO:0030674 protein-macromolecule adaptor activity	IDA PMID:7509083 Sequential interactions of the TCR with two distinct cytopla...	MODIFY	Summary: Specify the receptor-complex adaptor activity. Reason: LCK-dependent phosphorylation of zeta ITAMs creates docking sites for ZAP70, physically assembling a receptor-proximal signaling complex (PMID:7509083). GO:0030159 describes the signaling-receptor-complex adaptor role more precisely than the broad macromolecule-adaptor parent. Zeta is the scaffold/ligand, not the kinase; GO:0005068 would incorrectly restrict the role to an intrinsically tyrosine-kinase receptor. Proposed replacements: signaling receptor complex adaptor activity Supporting Evidence: PMID:7509083 through both of the ZAP-70 Src homology 2 domains
GO:0032395 MHC class II receptor activity	IDA PMID:1323144 Activation-induced ubiquitination of the T cell antigen rece...	ACCEPT	Summary: Preserve contribution to the assembled MHC-II receptor activity. Reason: The restored source qualifier is contributes_to, not enables. CD247 supplies signaling capacity to the TCR complex; it does not directly recognize peptide-MHC II. Five cached human GO-CAM models explicitly assign GO:0032395 to the alpha-beta TCR complex with CD247 as a part, consistent with this interpretation. The original PMID:1323144 cache exposes receptor ubiquitination but not the complete ligand-specific assay, so that source-level detail remains unverified; the existing curator judgment is retained with this boundary.
GO:0033001 Fc-gamma receptor III complex	IDA PMID:1825220 Analysis of Fc gamma RIII (CD16) membrane expression and ass...	ACCEPT	Summary: CD247 is a signaling component of the CD16 receptor complex. Reason: The PMID:1825220 abstract reports COS7 cotransfection, cytoplasmic truncations and transmembrane mutations supporting CD16-zeta association. Later human CD16A reconstitution in PMID:28652325 independently confirms the assembly. The complex term does not assign Fc recognition to CD247.
GO:0033001 Fc-gamma receptor III complex	IDA PMID:28652325 Transmembrane features governing Fc receptor CD16A assembly ...	ACCEPT	Summary: Human CD247 assembles with CD16A signaling complexes. Reason: The canonical PMID:28652325 cache contains abstract/discussion; independently inspected official PMC Results/captions (https://pmc.ncbi.nlm.nih.gov/articles/PMC5514760/) show human adaptor dimers in cotranslation/pull-down and cellular surface-expression assays. Post-mixing and receptor-mutant controls distinguish assembly from detergent-induced association. Murine CD247 is not assumed interchangeable with the human CD16A adaptor. Supporting Evidence: PMID:28652325 CD16A associates equally well with human CD247 and FcεR1γ homodimers
GO:0038094 Fc-gamma receptor signaling pathway	IDA PMID:8478617 Physical and functional association of p56lck with Fc gamma ...	ACCEPT	Summary: The zeta signaling module participates directly in Fc-gamma receptor signaling. Reason: PMID:8478617 reports NK-cell CD16 signaling and physical/functional interaction among LCK, CD16 and zeta in a heterologous reconstitution. Human CD16-zeta assembly is independently supported. CD247 provides signaling motifs, while LCK performs phosphorylation; no Fc ligand-binding or intrinsic kinase activity is inferred.
GO:0042101 T cell receptor complex	IDA PMID:8176201 Differential expression of ZAP-70 and Syk protein tyrosine k...	ACCEPT	Summary: Retain CD247 membership in the TCR complex. Reason: The PMID:8176201 abstract focuses on human/mouse Syk-family kinase expression and heterologous zeta association. Although the original subunit-membership control was not reread in full, independent human TCR-CD3 structure and assembly establish the same membership. Retain the experimental annotation with curator deference.
GO:0042101 T cell receptor complex	TAS PMID:3785426 A new subunit of the human T-cell antigen receptor complex.	ACCEPT	Summary: Human zeta is a TCR-associated chain. Reason: The complete 1986 abstract identifies the human counterpart of the murine zeta chain in the TCR complex. Modern human structure independently supports membership; this TAS is not an isoform-specific assembly assertion.
GO:0042105 alpha-beta T cell receptor complex	IBA GO_REF:0000033	ACCEPT	Summary: Retain the curated inherited alpha-beta TCR membership. Reason: The PTN008492834 IBA reflects a PAINT ancestral-node judgment and includes experimentally supported mouse and human members. Target self-inclusion is legitimate descendant evidence, not circularity. The full tree/MSA and IBD placement were not reconstructed; direct human cryo-EM independently establishes this complex membership.
GO:0042105 alpha-beta T cell receptor complex	IDA PMID:31461748 Structural basis of assembly of the human T cell receptor-CD...	ACCEPT	Summary: Human CD247 is the zeta-zeta module of the alpha-beta TCR-CD3 complex. Reason: PMID:31461748 describes one TCRalpha-beta, one CD3gamma-epsilon, one CD3delta-epsilon and one zeta-zeta module in the reconstituted human structure. Retain the separate IDA assertion. The official correction adds omitted construct/sequence information (https://doi.org/10.1038/s41586-021-03245-4); a universal native stoichiometry across all receptor states is not inferred.
GO:0042105 alpha-beta T cell receptor complex	IDA PMID:9485181 Assembly of the TCR/CD3 complex: CD3 epsilon/delta and CD3 e...	ACCEPT	Summary: The older human assembly study supports zeta membership. Reason: PMID:9485181 studies human Jurkat cells and thymocytes and directly addresses CD3-zeta-dependent receptor assembly. Retain membership while rejecting use of its proposed double-TCR stoichiometry as the current structural model; the later human structure in PMID:31461748 gives different stoichiometry.
GO:0042105 alpha-beta T cell receptor complex	IEA GO_REF:0000107	ACCEPT	Summary: The ortholog transfer agrees with directly observed human TCR membership. Reason: The source derives from mouse P24161 through Ensembl Compara. Human structure independently establishes membership, so the annotation does not depend on assuming every mouse receptor context or complex stoichiometry transfers unchanged.
GO:0042105 alpha-beta T cell receptor complex	IPI PMID:31461748 Structural basis of assembly of the human T cell receptor-CD...	ACCEPT	Summary: Human CD247 is the zeta-zeta module of the alpha-beta TCR-CD3 complex. Reason: PMID:31461748 describes one TCRalpha-beta, one CD3gamma-epsilon, one CD3delta-epsilon and one zeta-zeta module in the reconstituted human structure. Retain the separate IPI assertion. The official correction adds omitted construct/sequence information (https://doi.org/10.1038/s41586-021-03245-4); a universal native stoichiometry across all receptor states is not inferred.
GO:0042105 alpha-beta T cell receptor complex	ISS GO_REF:0000114	ACCEPT	Summary: Homologous-complex inference supports alpha-beta TCR membership. Reason: The CPX-6482 transfer is independently corroborated by the actual human TCR-CD3 structure. The transfer record was not fully reconstructed; its composition inference is retained without creating an extra enzymatic function.
GO:0042106 gamma-delta T cell receptor complex	NAS PMID:16418397 Stoichiometry of the murine gammadelta T cell receptor.	ACCEPT	Summary: Retain gamma-delta TCR membership with subset and species limits. Reason: The original PMID:16418397 experiments are explicitly murine and compare CD3gamma/delta stoichiometry; zeta-zeta is part of the assembly models rather than a new human structure. The reviewed human UniProt record and gamma-delta TCR synthesis in PMID:30976362 independently support CD247 membership. Some activated or tissue-specific gamma-delta receptors can use FcRgamma instead, so this is not an obligatory composition claim for every subset. Supporting Evidence: PMID:16418397 one TCRγδ heterodimer, two CD3γɛ dimers, and one TCRζ homodimer
GO:0042802 identical protein binding	IDA PMID:14967045 Homooligomerization of the cytoplasmic domain of the T cell ...	KEEP AS NON CORE	Summary: Retain broad self-association of isolated cytoplasmic domains. Reason: PMID:14967045 reports purified zeta cytoplasmic-domain monomer-dimer-tetramer equilibrium at approximately 10 micromolar and 1 millimolar dissociation scales. This establishes self-association but does not make a physiological cytoplasmic tetramer a core mechanism. The native transmembrane homodimer has distinct direct structural support. Supporting Evidence: PMID:14967045 two-step monomer-dimer-tetramer fast dynamic equilibrium
GO:0042802 identical protein binding	IPI PMID:17055436 The structure of the zetazeta transmembrane dimer reveals fe...	MODIFY	Summary: Refine self-binding to the directly characterized transmembrane homodimer. Reason: PMID:17055436 reports an NMR transmembrane dimer and mutational tests of dimerization/assembly. Selected external primary Results/Methods (https://pmc.ncbi.nlm.nih.gov/articles/PMC3466601/) distinguish the E. coli-expressed 33-residue NMR peptide from full-length human zeta in ER-microsome assembly assays. The canonical HTML cache is partial. The informative activity is homodimerization, not an inferred tetramer or channel. Proposed replacements: protein homodimerization activity Supporting Evidence: PMID:17055436 three polar positions are critical for zetazeta dimerization
GO:0042802 identical protein binding	IPI PMID:24502978 β-Arrestin-1 mediates the TCR-triggered re-routing of distal...	KEEP AS NON CORE	Summary: Retain the self-association assertion without assigning a source-specific assembly mechanism. Reason: PMID:24502978 available prose concerns ARRB1-dependent TCR trafficking; its exact CD247 self-interaction control was not recovered. Independent direct zeta homodimer structures substantiate self-binding, but neither the source-specific interface nor a higher-order oligomer is established by this partial cache.
GO:0042803 protein homodimerization activity	IDA PMID:1825220 Analysis of Fc gamma RIII (CD16) membrane expression and ass...	ACCEPT	Summary: Retain CD247 homodimer formation in receptor assembly. Reason: The original PMID:1825220 abstract supports zeta-dependent receptor assembly but does not expose all dimer controls. Independent primary NMR/mutational evidence in PMID:17055436 and human CD16A reconstitution in PMID:28652325 establish the homodimer. The source assay limitation is kept distinct from the well-supported molecular activity.
GO:0042803 protein homodimerization activity	IDA PMID:2532305 Co-association of CD3 zeta with a receptor (CD16) for IgG Fc...	ACCEPT	Summary: Human NK-cell CD16 associates with the zeta homodimer. Reason: PMID:2532305 explicitly reports a zeta homodimer coassociated with CD16 on human NK-cell membranes. This supports the dimeric receptor signaling module, without inferring that all CD247 complexes have identical composition. Supporting Evidence: PMID:2532305 specifically associates with the CD3 zeta homodimer
GO:0042803 protein homodimerization activity	IDA PMID:28652325 Transmembrane features governing Fc receptor CD16A assembly ...	ACCEPT	Summary: Human CD247 homodimers participate in CD16A assembly. Reason: Selected official PMC Results/captions for PMID:28652325 compare zeta-zeta, gamma-gamma and gamma-zeta signaling dimers in reconstituted receptor assembly (https://pmc.ncbi.nlm.nih.gov/articles/PMC5514760/). The experiment establishes alternative modules, not a requirement for every human CD16A complex to contain zeta. Supporting Evidence: PMID:28652325 form disulphide-linked homodimers
GO:0046629 gamma-delta T cell activation	NAS PMID:30976362 T cell receptor signaling for γδT cell development.	KEEP AS NON CORE	Summary: Retain the gamma-delta T-cell activation context. Reason: The review PMID:30976362 supports receptor-mediated activation, with substantial species/subset variation and possible FcRgamma substitution. This cellular outcome is downstream of the defined assembly/adaptor mechanism and does not warrant a separate core activity.
GO:0046631 alpha-beta T cell activation	NAS PMID:29789755 Regulatory mechanisms in T cell receptor signalling.	KEEP AS NON CORE	Summary: Retain alpha-beta T-cell activation as the cellular outcome. Reason: PMID:29789755 synthesizes T-cell development and receptor activation. CD247 molecular participation is captured more precisely by receptor assembly and TCR signaling; this broad activation outcome is retained outside the core process list.
GO:0046982 protein heterodimerization activity	IDA PMID:1825220 Analysis of Fc gamma RIII (CD16) membrane expression and ass...	ACCEPT	Summary: CD247 can form a mixed signaling dimer with FcRgamma. Reason: The source concerns CD16 association with zeta and FcRgamma. The exact mixed-dimer control is not exposed by the 1991 abstract, but actual human reconstitution in PMID:28652325 independently demonstrates gamma-zeta as well as homodimer modules. Retain heterodimerization without generalizing interchangeability to mouse CD247.
GO:0050852 T cell receptor signaling pathway	IBA GO_REF:0000033	ACCEPT	Summary: Retain the PAINT-supported inherited TCR signaling role. Reason: PTN008492834 is a curated ancestral assertion, not a simple donor-count vote. Human self-inclusion is expected because direct target evidence helps ground the node. The full tree/MSA was not reconstructed; human ITAM recruitment and receptor structure independently support the function.
GO:0050852 T cell receptor signaling pathway	IDA PMID:1323144 Activation-induced ubiquitination of the T cell antigen rece...	ACCEPT	Summary: Receptor-triggered CD247 events occur in the TCR pathway. Reason: PMID:1323144 directly reports activation-induced TCR-zeta ubiquitination and phosphorylation context. CD247 is not assigned ubiquitin-ligase activity. Its direct signaling participation is independently established by the ITAM recruitment assays in PMID:7509083; source-level ligand-specific controls were not inspected in the abstract-only cache.
GO:0050852 T cell receptor signaling pathway	IDA PMID:35271814 Cholesterol inhibits TCR signaling by directly restricting T...	ACCEPT	Summary: Human CD247 transmembrane conformation couples receptor assembly to signaling. Reason: The complete abstract of PMID:35271814 describes cholesterol/CS-bound structures and mutations that move the proximal CD3zeta transmembrane C terminus and activate TCR-CD3 in human cells. This supports direct receptor signaling participation; the complete Methods and all mutant controls were not inspected, and no separate intrinsic lipid-regulator activity is asserted. Supporting Evidence: PMID:35271814 thereby activating the TCR-CD3 complex in human cells
GO:0050852 T cell receptor signaling pathway	IDA PMID:7509083 Sequential interactions of the TCR with two distinct cytopla...	ACCEPT	Summary: Phosphorylated CD247 ITAMs recruit the proximal TCR signaling kinase. Reason: The source establishes sequential LCK-dependent phosphorylation and ZAP70 SH2-mediated recruitment. Selected reproduced primary controls distinguish human Jurkat from LCK-deficient J.CaM1 cells (author copy at https://www.researchgate.net/publication/267042930_Pillars_Article_Sequential_Interactions_of_the_TCR_with_Two_Distinct_Cytoplasmic_Tyrosine_kinases_Science_1994_263_1136-1139). CD247 supplies the docking platform rather than performing the kinase reaction.
GO:0050852 T cell receptor signaling pathway	IEA GO_REF:0000107	ACCEPT	Summary: The electronic ortholog inference matches direct human TCR signaling evidence. Reason: The source preserves mouse P24161. Direct human phosphorylation/recruitment and structural perturbation experiments independently establish the same process; no mouse-only effector mechanism is transferred by this decision.
GO:0050852 T cell receptor signaling pathway	NAS PMID:29789755 Regulatory mechanisms in T cell receptor signalling.	ACCEPT	Summary: Retain the reviewed receptor-signaling process. Reason: PMID:29789755 provides secondary synthesis of TCR biology. Direct CD247 ITAM recruitment and membrane assembly independently establish TCR signaling, while the source retains its NAS code and is not represented as a new human primary assay.
GO:0050852 T cell receptor signaling pathway	NAS PMID:30976362 T cell receptor signaling for γδT cell development.	ACCEPT	Summary: Retain the reviewed receptor-signaling process. Reason: PMID:30976362 provides secondary synthesis of TCR biology. Direct CD247 ITAM recruitment and membrane assembly independently establish TCR signaling, while the source retains its NAS code and is not represented as a new human primary assay. Supporting Evidence: PMID:30976362 recruitment of Zap70 to the αβTCR-CD3 complexes
GO:0051259 protein complex oligomerization	EXP PMID:14967045 Homooligomerization of the cytoplasmic domain of the T cell ...	KEEP AS NON CORE	Summary: Retain the experimentally observed isolated-domain oligomerization. Reason: PMID:14967045 describes concentration-dependent purified cytoplasmic-domain association, including a high-concentration tetrameric state, without an ordered fold. This EXP assertion is retained as an in-vitro self-association result; it does not establish a native tetrameric signaling unit. Physiological receptor assembly is supported separately by transmembrane-dimer experiments.
GO:0051259 protein complex oligomerization	IPI PMID:14967045 Homooligomerization of the cytoplasmic domain of the T cell ...	KEEP AS NON CORE	Summary: Retain the experimentally observed isolated-domain oligomerization. Reason: PMID:14967045 describes concentration-dependent purified cytoplasmic-domain association, including a high-concentration tetrameric state, without an ordered fold. This IPI assertion is retained as an in-vitro self-association result; it does not establish a native tetrameric signaling unit. Physiological receptor assembly is supported separately by transmembrane-dimer experiments.
GO:0065003 protein-containing complex assembly	IDA PMID:9485181 Assembly of the TCR/CD3 complex: CD3 epsilon/delta and CD3 e...	ACCEPT	Summary: CD247 contributes physical contacts to receptor-complex assembly. Reason: The human assembly experiments in PMID:9485181 establish a role for the zeta module, although their double-TCR stoichiometric model is not adopted. Independent transmembrane mutagenesis in PMID:17055436 and human structural evidence in PMID:31461748 support direct assembly work by CD247 rather than merely a requirement inferred from depletion. Supporting Evidence: PMID:9485181 the CD3 zeta homodimer is added to allow export
GO:0098797 plasma membrane protein complex	IEA GO_REF:0000117	ACCEPT	Summary: The electronic plasma-membrane complex assignment is independently established. Reason: ARBA00028841 provides the source inference; its detailed decision rule was not inspected. Human TCR-CD3 and CD16A complexes directly establish CD247 as a membrane-complex subunit, so acceptance does not require assigning it the activity of every partner.
GO:1990782 protein tyrosine kinase binding	IPI PMID:8681956 Phosphorylated T cell receptor zeta-chain and ZAP70 tandem S...	ACCEPT	Summary: Retain binding to the cytoplasmic tyrosine kinase LCK. Reason: The source PMID:8681956 focuses on ZAP70-SH2 binding and uses LCK-phosphorylated recombinant zeta; its exact original P06239 interaction control was not inspected. Independent direct LCK SH2/SH3-zeta binding in PMID:8626561 supports this same partner/class relation. Preserve the immutable LCK supporting entity rather than silently substituting ZAP70.
GO:2000010 positive regulation of protein localization to cell surface	IEA GO_REF:0000107	ACCEPT	Summary: Receptor assembly by CD247 promotes partner surface expression. Reason: Although this electronic source transfers from mouse, human CD16A cotransfection and reconstitution independently show CD247-mediated receptor assembly and surface expression (PMIDs:2532305, 1825220 and 28652325). This is direct physical assembly work, not a generic expression correlation. Certain CD16A mutants escape retention without an adaptor, so the effect is not universal across constructs; the original acts_upstream_of_or_within qualifier is preserved.

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Perplexity

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