CD3G encodes CD3 gamma, a single-pass signaling subunit of the T cell antigen receptor. Its extracellular immunoglobulin-like domain pairs noncovalently with CD3 epsilon, and extracellular and transmembrane contacts help organize the receptor complex. Human alpha-beta and reconstituted gamma-delta TCR complexes contain this gamma-epsilon module. The cytoplasmic tail carries one ITAM whose phosphorylation permits signaling-effector recruitment, including ZAP70, while a separate dileucine sorting signal engages endocytic adaptors and helps control receptor internalization. CD3 gamma promotes normal surface receptor abundance and efficient ligand- or PKC-stimulated trafficking; some mature human T cells retain surface receptors and constitutive internalization without it. Biallelic defects cause variable immunodeficiency, sometimes accompanied by autoimmunity and altered regulatory-T-cell function.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0002250 adaptive immune response | NAS PMID:29789755 Regulatory mechanisms in T cell receptor signalling. | ACCEPT | Summary: CD3G participates in adaptive T cell responses as a receptor signaling subunit. Reason: The cited review concerns TCR signaling; the human CD3G assembly and deficiency evidence independently establishes the subunit role. This accepts the author-statement process without claiming that the review performs a CD3G-specific experiment. |
| GO:0002250 adaptive immune response | NAS PMID:30976362 T cell receptor signaling for Ξ³Ξ΄T cell development. | ACCEPT | Summary: CD3G contributes to adaptive responses of gamma-delta T cells. Reason: The gamma-delta signaling review is consistent with a physical CD3G-containing receptor complex. Reconstituted human gamma-delta structures provide separate human composition evidence; mouse-only stoichiometry is not generalized to all human receptors. |
| GO:0004888 transmembrane signaling receptor activity | IBA GO_REF:0000033 | ACCEPT | Summary: CD3G supplies a signal-transducing component of the transmembrane TCR complex. Reason: Retain the curated PAINT inheritance, corroborated by human assembly and ITAM evidence. The actual PTN000060280 IBD placement was not reconstructed; donor count and the target itself in WITH/FROM do not undermine it. Ligand specificity resides in the clonotypic TCR chains; the core states the complex contribution explicitly. |
| GO:0004888 transmembrane signaling receptor activity | IC PMID:9485181 Assembly of the TCR/CD3 complex: CD3 epsilon/delta and CD3 e... | ACCEPT | Summary: CD3G contributes to the assembled transmembrane signaling receptor. Reason: The IC inference from alpha-beta TCR membership agrees with human CD3 gamma-epsilon association. The historical double-TCR model in the cited paper is not adopted: the later human structure resolves one alpha-beta pair with the signaling dimers. No intrinsic peptide-MHC recognition by CD3G is asserted. |
| GO:0004888 transmembrane signaling receptor activity | IEA GO_REF:0000002 | ACCEPT | Summary: The CD3-family domain mapping is consistent with receptor signal transduction. Reason: InterPro supplies an electronic association; human complex structure and phosphorylated ITAM binding provide target evidence for the signaling-subunit role. Preserve enables in the source object, while distinguishing the complex contribution in the core. |
| GO:0004888 transmembrane signaling receptor activity | IMP PMID:12794121 TCR dynamics in human mature T lymphocytes lacking CD3 gamma... | ACCEPT | Summary: Human CD3G supports normal surface TCR assembly and signaling competence. Reason: The human deficiency and retroviral-restoration study supports a functional receptor subunit. Residual surface TCR persists without CD3G, so the evidence does not establish an absolute assembly requirement in every mature T cell or direct antigen binding by CD3G. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: The curated human interaction with TMEM262 is retained as a non-core association. Reason: The exact E9PQX1 edge is recorded in GOA and the reviewed UniProt interaction list. The complete HuRI abstract establishes the screen context, but its original target construct/table was not independently inspected. No evidence supports calling this edge false or merely membrane-protein background. Retain the qualitative binding under the project instruction; partner identity alone does not establish a more specific interface or adaptor function. Supporting Evidence: |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: The curated human interaction with LAT isoform 2 is retained as a non-core association. Reason: The exact O43561-2 edge is recorded in GOA and the reviewed UniProt interaction list. The complete HuRI abstract establishes the screen context, but its original target construct/table was not independently inspected. No evidence supports calling this edge false or merely membrane-protein background. Retain the qualitative binding under the project instruction; partner identity alone does not establish a more specific interface or adaptor function. Supporting Evidence: |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: The curated human interaction with MAL is retained as a non-core association. Reason: The exact P21145 edge is recorded in GOA and the reviewed UniProt interaction list. The complete HuRI abstract establishes the screen context, but its original target construct/table was not independently inspected. No evidence supports calling this edge false or merely membrane-protein background. Retain the qualitative binding under the project instruction; partner identity alone does not establish a more specific interface or adaptor function. Supporting Evidence: |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: The curated human interaction with EMP1 is retained as a non-core association. Reason: The exact P54849 edge is recorded in GOA and the reviewed UniProt interaction list. The complete HuRI abstract establishes the screen context, but its original target construct/table was not independently inspected. No evidence supports calling this edge false or merely membrane-protein background. Retain the qualitative binding under the project instruction; partner identity alone does not establish a more specific interface or adaptor function. Supporting Evidence: |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: The curated human interaction with PMP22 is retained as a non-core association. Reason: The exact Q01453 edge is recorded in GOA and the reviewed UniProt interaction list. The complete HuRI abstract establishes the screen context, but its original target construct/table was not independently inspected. No evidence supports calling this edge false or merely membrane-protein background. Retain the qualitative binding under the project instruction; partner identity alone does not establish a more specific interface or adaptor function. Supporting Evidence: |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: The curated human interaction with KTN1 isoform 3 is retained as a non-core association. Reason: The exact Q86UP2-3 edge is recorded in GOA and the reviewed UniProt interaction list. The complete HuRI abstract establishes the screen context, but its original target construct/table was not independently inspected. No evidence supports calling this edge false or merely membrane-protein background. Retain the qualitative binding under the project instruction; partner identity alone does not establish a more specific interface or adaptor function. Supporting Evidence: |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: The curated human interaction with TMEM54 is retained as a non-core association. Reason: The exact Q969K7 edge is recorded in GOA and the reviewed UniProt interaction list. The complete HuRI abstract establishes the screen context, but its original target construct/table was not independently inspected. No evidence supports calling this edge false or merely membrane-protein background. Retain the qualitative binding under the project instruction; partner identity alone does not establish a more specific interface or adaptor function. Supporting Evidence: |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: The curated human interaction with TMEM203 is retained as a non-core association. Reason: The exact Q969S6 edge is recorded in GOA and the reviewed UniProt interaction list. The complete HuRI abstract establishes the screen context, but its original target construct/table was not independently inspected. No evidence supports calling this edge false or merely membrane-protein background. Retain the qualitative binding under the project instruction; partner identity alone does not establish a more specific interface or adaptor function. Supporting Evidence: |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: The curated human interaction with TMEM19 is retained as a non-core association. Reason: The exact Q96HH6 edge is recorded in GOA and the reviewed UniProt interaction list. The complete HuRI abstract establishes the screen context, but its original target construct/table was not independently inspected. No evidence supports calling this edge false or merely membrane-protein background. Retain the qualitative binding under the project instruction; partner identity alone does not establish a more specific interface or adaptor function. Supporting Evidence: |
| GO:0005886 plasma membrane | EXP PMID:8636209 Role of CD3 gamma in T cell receptor assembly. | ACCEPT | Summary: Human CD3G reaches the cell surface within assembled TCR complexes. Reason: The human CD3G mutational assembly study supports the curated membrane localization. Its abstract directly tests CD3G extracellular and transmembrane regions; this is not an inference from an unrelated paralog title. |
| GO:0005886 plasma membrane | IDA GO_REF:0000052 | ACCEPT | Summary: Plasma-membrane localization is supported independently of the imaging curation. Reason: The GO_REF identifies immunofluorescence curation, without exposing its target image here. Human receptor assembly and surface-labeling evidence separately supports this location, so no original image inspection is implied. |
| GO:0005886 plasma membrane | IDA PMID:11390434 The transmembrane adaptor protein TRIM regulates T cell rece... | ACCEPT | Summary: CD3G is present in surface-labeled human TCR complexes. Reason: Selected original Methods and Results include surface biotinylation of Jurkat cells, anti-CD3 immunoprecipitation and CD3 gamma reprecipitation. The TRIM-focused title does not exclude the assayed target. Preserve the source is_active_in qualifier without assigning TRIM-dependent calcium effects to CD3G. Supporting Evidence: |
| GO:0005886 plasma membrane | IDA PMID:31461748 Structural basis of assembly of the human T cell receptor-CD... | ACCEPT | Summary: The human TCR structure locates CD3G across the membrane. Reason: The directly characterized complex includes the CD3 gamma-epsilon pair with transmembrane contacts. It supports membrane localization and assembly, without assigning the antigen-binding surface to CD3G. Supporting Evidence: |
| GO:0005886 plasma membrane | IEA GO_REF:0000044 | ACCEPT | Summary: The UniProt subcellular-location mapping correctly places CD3G at the plasma membrane. Reason: The reviewed single-pass topology and human surface receptor data agree with the conservative SL-0039 mapping. |
| GO:0005886 plasma membrane | NAS PMID:30976362 T cell receptor signaling for Ξ³Ξ΄T cell development. | ACCEPT | Summary: CD3G is a membrane component of gamma-delta TCR complexes. Reason: The review attribution is corroborated by separate human gamma-delta TCR-CD3 structures. This is a location assertion, not a claim that every species uses the same dimer stoichiometry. |
| GO:0005886 plasma membrane | NAS PMID:9485181 Assembly of the TCR/CD3 complex: CD3 epsilon/delta and CD3 e... | ACCEPT | Summary: The associated CD3 gamma-epsilon module is part of the surface TCR. Reason: Human Jurkat/thymocyte association data and the later human structural complex support the location. The earlier proposed double-TCR stoichiometry is not required for it. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-198955 | ACCEPT | Summary: The Reactome antigen-presenting MHC class I engagement context retains plasma-membrane CD3G. Reason: This source row asserts compartment membership of the receptor-containing assembly. Human surface and structural evidence supports that compartment; the event does not make CD3G the kinase, phosphatase, ligand-binding TCR chain or the other protein named in its title. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-202165 | ACCEPT | Summary: The Reactome ITAM phosphorylation context retains plasma-membrane CD3G. Reason: This source row asserts compartment membership of the receptor-containing assembly. Human surface and structural evidence supports that compartment; the event does not make CD3G the kinase, phosphatase, ligand-binding TCR chain or the other protein named in its title. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-202168 | ACCEPT | Summary: The Reactome LCK-dependent ZAP70 phosphorylation context retains plasma-membrane CD3G. Reason: This source row asserts compartment membership of the receptor-containing assembly. Human surface and structural evidence supports that compartment; the event does not make CD3G the kinase, phosphatase, ligand-binding TCR chain or the other protein named in its title. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-202174 | ACCEPT | Summary: The Reactome ZAP70 activation context retains plasma-membrane CD3G. Reason: This source row asserts compartment membership of the receptor-containing assembly. Human surface and structural evidence supports that compartment; the event does not make CD3G the kinase, phosphatase, ligand-binding TCR chain or the other protein named in its title. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-202214 | ACCEPT | Summary: The Reactome CD45-dependent LCK dephosphorylation context retains plasma-membrane CD3G. Reason: This source row asserts compartment membership of the receptor-containing assembly. Human surface and structural evidence supports that compartment; the event does not make CD3G the kinase, phosphatase, ligand-binding TCR chain or the other protein named in its title. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-202216 | ACCEPT | Summary: The Reactome SLP76 phosphorylation context retains plasma-membrane CD3G. Reason: This source row asserts compartment membership of the receptor-containing assembly. Human surface and structural evidence supports that compartment; the event does not make CD3G the kinase, phosphatase, ligand-binding TCR chain or the other protein named in its title. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-202233 | ACCEPT | Summary: The Reactome CSK-dependent LCK inactivation context retains plasma-membrane CD3G. Reason: This source row asserts compartment membership of the receptor-containing assembly. Human surface and structural evidence supports that compartment; the event does not make CD3G the kinase, phosphatase, ligand-binding TCR chain or the other protein named in its title. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-202245 | ACCEPT | Summary: The Reactome LAT phosphorylation context retains plasma-membrane CD3G. Reason: This source row asserts compartment membership of the receptor-containing assembly. Human surface and structural evidence supports that compartment; the event does not make CD3G the kinase, phosphatase, ligand-binding TCR chain or the other protein named in its title. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-202248 | ACCEPT | Summary: The Reactome PLCG1 phosphorylation context retains plasma-membrane CD3G. Reason: This source row asserts compartment membership of the receptor-containing assembly. Human surface and structural evidence supports that compartment; the event does not make CD3G the kinase, phosphatase, ligand-binding TCR chain or the other protein named in its title. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-202291 | ACCEPT | Summary: The Reactome LCK activation context retains plasma-membrane CD3G. Reason: This source row asserts compartment membership of the receptor-containing assembly. Human surface and structural evidence supports that compartment; the event does not make CD3G the kinase, phosphatase, ligand-binding TCR chain or the other protein named in its title. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-202307 | ACCEPT | Summary: The Reactome PKC-theta conformational activation context retains plasma-membrane CD3G. Reason: This source row asserts compartment membership of the receptor-containing assembly. Human surface and structural evidence supports that compartment; the event does not make CD3G the kinase, phosphatase, ligand-binding TCR chain or the other protein named in its title. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-202344 | ACCEPT | Summary: The Reactome ZAP70 recruitment context retains plasma-membrane CD3G. Reason: This source row asserts compartment membership of the receptor-containing assembly. Human surface and structural evidence supports that compartment; the event does not make CD3G the kinase, phosphatase, ligand-binding TCR chain or the other protein named in its title. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-2029268 | ACCEPT | Summary: The plasma-membrane assertion is retained; the PLCG activation context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-2029270 | ACCEPT | Summary: The plasma-membrane assertion is retained; the PLCG membrane recruitment context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-2029271 | ACCEPT | Summary: The plasma-membrane assertion is retained; the PI3K lipid phosphorylation context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-2029272 | ACCEPT | Summary: The plasma-membrane assertion is retained; the PLCG release context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-2029273 | ACCEPT | Summary: The plasma-membrane assertion is retained; the PI3K-SYK association context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-2029451 | ACCEPT | Summary: The plasma-membrane assertion is retained; the VAV-dependent RAC1 activation context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-2029452 | ACCEPT | Summary: The plasma-membrane assertion is retained; the SYK recruitment context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-2029453 | ACCEPT | Summary: The plasma-membrane assertion is retained; the VAV phosphorylation context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-2029455 | ACCEPT | Summary: The plasma-membrane assertion is retained; the FCGRIA cross-linking context is bounded. Reason: Official Reactome R-HSA-2029455 describes the Fc receptor gamma chain, yet its input chain R-HSA-2029093 to R-HSA-1861690 to R-HSA-197917 explicitly links CD3G/P09693. Official FCER1G identity is instead P30273. This is a concrete source-mapping inconsistency, not evidence against independently established CD3G plasma-membrane localization. Preserve the original TAS source and retain the location using the human TCR evidence, without adopting Fc-receptor participation. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-2029457 | ACCEPT | Summary: The plasma-membrane assertion is retained; the FCGRIIIA cross-linking context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-2029458 | ACCEPT | Summary: The plasma-membrane assertion is retained; the VAV1 recruitment context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-2029459 | ACCEPT | Summary: The plasma-membrane assertion is retained; the Fc receptor partitioning/phosphorylation context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-2029467 | ACCEPT | Summary: The plasma-membrane assertion is retained; the CRKII-DOCK180-ELMO-dependent RAC1 activation context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-2029476 | ACCEPT | Summary: The plasma-membrane assertion is retained; the myosin-associated phagosome formation context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-2197697 | ACCEPT | Summary: The plasma-membrane assertion is retained; the CRKII-DOCK180-ELMO recruitment context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-389758 | ACCEPT | Summary: The Reactome PD-1-associated phosphatase action on CD3-zeta context retains plasma-membrane CD3G. Reason: This source row asserts compartment membership of the receptor-containing assembly. Human surface and structural evidence supports that compartment; the event does not make CD3G the kinase, phosphatase, ligand-binding TCR chain or the other protein named in its title. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-8850326 | ACCEPT | Summary: The Reactome CD1-presented self-lipid recognition context retains plasma-membrane CD3G. Reason: This source row asserts compartment membership of the receptor-containing assembly. Human surface and structural evidence supports that compartment; the event does not make CD3G the kinase, phosphatase, ligand-binding TCR chain or the other protein named in its title. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-8850356 | ACCEPT | Summary: The Reactome CD1-presented microbial-lipid recognition context retains plasma-membrane CD3G. Reason: This source row asserts compartment membership of the receptor-containing assembly. Human surface and structural evidence supports that compartment; the event does not make CD3G the kinase, phosphatase, ligand-binding TCR chain or the other protein named in its title. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-8852200 | ACCEPT | Summary: The Reactome PTPN22-dependent LCK inactivation context retains plasma-membrane CD3G. Reason: This source row asserts compartment membership of the receptor-containing assembly. Human surface and structural evidence supports that compartment; the event does not make CD3G the kinase, phosphatase, ligand-binding TCR chain or the other protein named in its title. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-8855381 | ACCEPT | Summary: The Reactome PTPN22-dependent ZAP70 dephosphorylation context retains plasma-membrane CD3G. Reason: This source row asserts compartment membership of the receptor-containing assembly. Human surface and structural evidence supports that compartment; the event does not make CD3G the kinase, phosphatase, ligand-binding TCR chain or the other protein named in its title. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-8866277 | ACCEPT | Summary: CD3G is a membrane cargo component in the AP-2 cargo binding context. Reason: The Reactome endocytic context is compatible with a surface TCR entering coated pits. It does not assign the named coat-remodeling or catalytic step to CD3G. Its sorting tail and human internalization data independently support the membrane itinerary. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-8867754 | ACCEPT | Summary: CD3G is a membrane cargo component in the BAR-protein recruitment context. Reason: The Reactome endocytic context is compatible with a surface TCR entering coated pits. It does not assign the named coat-remodeling or catalytic step to CD3G. Its sorting tail and human internalization data independently support the membrane itinerary. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-8867756 | ACCEPT | Summary: CD3G is a membrane cargo component in the cargo/CLASP recruitment context. Reason: The Reactome endocytic context is compatible with a surface TCR entering coated pits. It does not assign the named coat-remodeling or catalytic step to CD3G. Its sorting tail and human internalization data independently support the membrane itinerary. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-8868071 | ACCEPT | Summary: CD3G is a membrane cargo component in the PIK3C2A recruitment context. Reason: The Reactome endocytic context is compatible with a surface TCR entering coated pits. It does not assign the named coat-remodeling or catalytic step to CD3G. Its sorting tail and human internalization data independently support the membrane itinerary. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-8868072 | ACCEPT | Summary: CD3G is a membrane cargo component in the phosphoinositide phosphorylation context. Reason: The Reactome endocytic context is compatible with a surface TCR entering coated pits. It does not assign the named coat-remodeling or catalytic step to CD3G. Its sorting tail and human internalization data independently support the membrane itinerary. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-8868230 | ACCEPT | Summary: CD3G is a membrane cargo component in the SNX9-associated actin machinery recruitment context. Reason: The Reactome endocytic context is compatible with a surface TCR entering coated pits. It does not assign the named coat-remodeling or catalytic step to CD3G. Its sorting tail and human internalization data independently support the membrane itinerary. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-8868236 | ACCEPT | Summary: CD3G is a membrane cargo component in the dynamin recruitment context. Reason: The Reactome endocytic context is compatible with a surface TCR entering coated pits. It does not assign the named coat-remodeling or catalytic step to CD3G. Its sorting tail and human internalization data independently support the membrane itinerary. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-8868648 | ACCEPT | Summary: CD3G is a membrane cargo component in the synaptojanin-dependent lipid hydrolysis context. Reason: The Reactome endocytic context is compatible with a surface TCR entering coated pits. It does not assign the named coat-remodeling or catalytic step to CD3G. Its sorting tail and human internalization data independently support the membrane itinerary. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-8868651 | ACCEPT | Summary: CD3G is a membrane cargo component in the endophilin-synaptojanin recruitment context. Reason: The Reactome endocytic context is compatible with a surface TCR entering coated pits. It does not assign the named coat-remodeling or catalytic step to CD3G. Its sorting tail and human internalization data independently support the membrane itinerary. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-8868661 | ACCEPT | Summary: CD3G is a membrane cargo component in the dynamin-mediated vesicle scission context. Reason: The Reactome endocytic context is compatible with a surface TCR entering coated pits. It does not assign the named coat-remodeling or catalytic step to CD3G. Its sorting tail and human internalization data independently support the membrane itinerary. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-9664261 | ACCEPT | Summary: The plasma-membrane assertion is retained; the SYK phosphorylation in a Leishmania-associated receptor complex context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-9664268 | ACCEPT | Summary: The plasma-membrane assertion is retained; the opsonized Leishmania recognition context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-9664270 | ACCEPT | Summary: The plasma-membrane assertion is retained; the FCGR3A-associated PLCG recruitment context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-9664271 | ACCEPT | Summary: The plasma-membrane assertion is retained; the PLCG release from FCGR3A context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-9664273 | ACCEPT | Summary: The plasma-membrane assertion is retained; the SYK recruitment to a pathogen-associated receptor context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-9664275 | ACCEPT | Summary: The plasma-membrane assertion is retained; the phosphorylation of receptor-associated signaling dimers context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-9664278 | ACCEPT | Summary: The plasma-membrane assertion is retained; the PLCG activation downstream of FCGR3A context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-9664406 | ACCEPT | Summary: The plasma-membrane assertion is retained; the IgG-pathogen binding to FCGR3A context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-9666425 | ACCEPT | Summary: The plasma-membrane assertion is retained; the VAV phosphorylation context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-9666426 | ACCEPT | Summary: The plasma-membrane assertion is retained; the CRKII-DOCK180-ELMO binding context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-9666428 | ACCEPT | Summary: The plasma-membrane assertion is retained; the DOCK180-mediated nucleotide exchange context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-9666430 | ACCEPT | Summary: The plasma-membrane assertion is retained; the VAV-mediated nucleotide exchange context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-9666435 | ACCEPT | Summary: The plasma-membrane assertion is retained; the VAV recruitment to SYK context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-9666458 | ACCEPT | Summary: The plasma-membrane assertion is retained; the receptor movement toward the parasitophorous vacuole context is bounded. Reason: Independent human TCR evidence establishes CD3G at the plasma membrane. The source Fc-receptor/CD3-dimer context does not independently resolve a CD3G-specific molecular role, and is not used to infer phagocytosis or enzyme activity. Preserve the location and original source while keeping that pathway attribution separate. |
| GO:0007163 establishment or maintenance of cell polarity | IMP PMID:12407027 Contribution of CD3 gamma to TCR regulation and signaling in... | KEEP AS NON CORE | Summary: CD3G deficiency perturbs polarization of activated human T cells. Reason: The complete source abstract explicitly reports defective adhesion and polarization on ICAM1-coated dishes in human CD3G-deficient cells. Retain this cellular consequence outside the molecular core; it does not show that CD3G directly reorganizes actin or binds ICAM1. Supporting Evidence: |
| GO:0007166 cell surface receptor signaling pathway | IBA GO_REF:0000033 | ACCEPT | Summary: Inherited cell-surface receptor signaling is consistent with the CD3G ITAM role. Reason: This PAINT assertion is retained as curated inheritance with independent human target support. The original IBD node was not reconstructed and the target appearing among descendant evidence is not circularity. |
| GO:0007166 cell surface receptor signaling pathway | IC PMID:9485181 Assembly of the TCR/CD3 complex: CD3 epsilon/delta and CD3 e... | ACCEPT | Summary: TCR complex membership supports CD3G participation in receptor signaling. Reason: The IC inference is consistent with human receptor assembly and CD3G-tail effector binding; it does not attribute a catalytic kinase activity to CD3G. |
| GO:0007166 cell surface receptor signaling pathway | IEA GO_REF:0000002 | ACCEPT | Summary: The electronic CD3-family signaling association is biologically supported. Reason: The target ITAM supplies phosphorylation-dependent effector docking within the membrane receptor. That target evidence corroborates the domain-derived process assignment. |
| GO:0007166 cell surface receptor signaling pathway | IMP PMID:12407027 Contribution of CD3 gamma to TCR regulation and signaling in... | ACCEPT | Summary: Human CD3G contributes to selected mature-T-cell signaling responses. Reason: The source abstract reports that several responses remain normal or nearly normal while adhesion/polarization and activation-induced death differ. Retain participation without claiming universal loss of TCR signaling after CD3G deficiency. Supporting Evidence: |
| GO:0009897 external side of plasma membrane | IBA GO_REF:0000033 | ACCEPT | Summary: The CD3G extracellular domain lies on the external face of the plasma membrane. Reason: The inherited location agrees with the reviewed type-I topology and the human extracellular CD3 gamma-epsilon structure. The PAINT IBD itself was not inspected; its short source list is not a reason to reject the compatible location. |
| GO:0015031 protein transport | IMP PMID:12794121 TCR dynamics in human mature T lymphocytes lacking CD3 gamma... | MODIFY | Summary: CD3G supports receptor internalization through its cytoplasmic sorting tail. Reason: Human deficiency slows early ligand-induced uptake, while constitutive internalization and degradation remain largely preserved. The tail motif supplies sorting-adaptor recognition, established separately by mutational and peptide-binding work; this is physical participation rather than necessity alone. Refine the broad transport assertion to receptor internalization, without claiming CD3G itself catalyzes vesicle scission. Proposed replacements: receptor internalization Supporting Evidence: PMID:12794121 early ligand-induced endocytosis was impaired |
| GO:0016020 membrane | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: The broad membrane term is correct but less informative than the established plasma-membrane role. Reason: The InterPro mapping agrees with single-pass topology. Retain this broad compartment as non-core; the specific plasma-membrane and TCR-complex locations are already represented. |
| GO:0030159 signaling receptor complex adaptor activity | NAS PMID:12794121 TCR dynamics in human mature T lymphocytes lacking CD3 gamma... | ACCEPT | Summary: CD3G physically supports the assembly and signaling capacity of the TCR complex. Reason: The human deficiency study supports this adaptor role, with independent mutational assembly, extracellular interface and ITAM-effector evidence. CD3G contributes contacts and a docking tail; it is not merely a downstream response marker. |
| GO:0030669 clathrin-coated endocytic vesicle membrane | TAS Reactome:R-HSA-8868658 | KEEP AS NON CORE | Summary: CD3G can occupy the coated-vesicle membrane during HSPA8-mediated uncoating. Reason: Retain the Reactome cargo-location annotation as a trafficking context. The sorting tail helps recruit the endocytic apparatus, but CD3G is not thereby the coat, chaperone, phosphatase or GTPase executing this event. |
| GO:0030669 clathrin-coated endocytic vesicle membrane | TAS Reactome:R-HSA-8868659 | KEEP AS NON CORE | Summary: CD3G can occupy the coated-vesicle membrane during auxilin recruitment. Reason: Retain the Reactome cargo-location annotation as a trafficking context. The sorting tail helps recruit the endocytic apparatus, but CD3G is not thereby the coat, chaperone, phosphatase or GTPase executing this event. |
| GO:0030669 clathrin-coated endocytic vesicle membrane | TAS Reactome:R-HSA-8868660 | KEEP AS NON CORE | Summary: CD3G can occupy the coated-vesicle membrane during HSPA8 recruitment. Reason: Retain the Reactome cargo-location annotation as a trafficking context. The sorting tail helps recruit the endocytic apparatus, but CD3G is not thereby the coat, chaperone, phosphatase or GTPase executing this event. |
| GO:0030669 clathrin-coated endocytic vesicle membrane | TAS Reactome:R-HSA-8868661 | KEEP AS NON CORE | Summary: CD3G can occupy the coated-vesicle membrane during dynamin-dependent scission. Reason: Retain the Reactome cargo-location annotation as a trafficking context. The sorting tail helps recruit the endocytic apparatus, but CD3G is not thereby the coat, chaperone, phosphatase or GTPase executing this event. |
| GO:0030669 clathrin-coated endocytic vesicle membrane | TAS Reactome:R-HSA-8869438 | KEEP AS NON CORE | Summary: CD3G can occupy the coated-vesicle membrane during coat-associated protein dissociation. Reason: Retain the Reactome cargo-location annotation as a trafficking context. The sorting tail helps recruit the endocytic apparatus, but CD3G is not thereby the coat, chaperone, phosphatase or GTPase executing this event. |
| GO:0030669 clathrin-coated endocytic vesicle membrane | TAS Reactome:R-HSA-8871193 | KEEP AS NON CORE | Summary: CD3G can occupy the coated-vesicle membrane during AAK1 dissociation/AP-2 dephosphorylation. Reason: Retain the Reactome cargo-location annotation as a trafficking context. The sorting tail helps recruit the endocytic apparatus, but CD3G is not thereby the coat, chaperone, phosphatase or GTPase executing this event. |
| GO:0030669 clathrin-coated endocytic vesicle membrane | TAS Reactome:R-HSA-8871194 | KEEP AS NON CORE | Summary: CD3G can occupy the coated-vesicle membrane during RAB5/GAPVD1 association. Reason: Retain the Reactome cargo-location annotation as a trafficking context. The sorting tail helps recruit the endocytic apparatus, but CD3G is not thereby the coat, chaperone, phosphatase or GTPase executing this event. |
| GO:0032395 MHC class II receptor activity | IDA PMID:1323144 Activation-induced ubiquitination of the T cell antigen rece... | ACCEPT | Summary: CD3G contributes to the activity of an appropriately MHC-II-restricted TCR complex. Reason: The preserved contributes_to qualifier makes this a complex-level claim, not direct MHC-II binding by CD3G. Eleven inspected human production GO-CAM activities include P09693 in the alpha-beta TCR complex enabling this activity, using shared PMID:1323144/PMID:31461748 provenance. The original paper cache alone does not reveal the clone-specific gamma assay; the positive curated complex model and human composition evidence are distinguished from a new verification of that experiment. |
| GO:0042105 alpha-beta T cell receptor complex | IBA GO_REF:0000033 | ACCEPT | Summary: CD3G is a conserved component of the alpha-beta TCR complex. Reason: Retain the curated PAINT inference, directly corroborated by human structural composition. The underlying ancestral placement remains uninspected rather than being reconstructed from the donor list. |
| GO:0042105 alpha-beta T cell receptor complex | IDA PMID:9485181 Assembly of the TCR/CD3 complex: CD3 epsilon/delta and CD3 e... | ACCEPT | Summary: Human CD3 gamma-epsilon dimers associate within the alpha-beta receptor complex. Reason: The cited human association study supports membership. Its historical double-receptor interpretation is superseded for the described human structural assembly by the later one-alpha-beta-pair complex. |
| GO:0042105 alpha-beta T cell receptor complex | IEA GO_REF:0000120 | ACCEPT | Summary: The combined electronic assignment to the alpha-beta TCR complex is independently supported. Reason: Preserve the ARBA and mouse orthology source entities. The actual human complex structure confirms CD3G membership without requiring an uninspected rule or donor assay to carry the conclusion. |
| GO:0042105 alpha-beta T cell receptor complex | IPI PMID:31461748 Structural basis of assembly of the human T cell receptor-CD... | ACCEPT | Summary: The human structural complex contains one CD3 gamma-epsilon pair. Reason: The cryo-EM assembly establishes direct physical membership in the alpha-beta receptor complex, rather than merely a deficiency phenotype. Supporting Evidence: |
| GO:0042106 gamma-delta T cell receptor complex | NAS PMID:16418397 Stoichiometry of the murine gammadelta T cell receptor. | ACCEPT | Summary: CD3G is also a component of human gamma-delta TCR complexes. Reason: The original NAS source measures murine stoichiometry; independent 2024 human structures establish gamma-epsilon membership. Their inclusion of delta-epsilon is kept separate from the older mouse architecture. |
| GO:0042110 T cell activation | NAS PMID:12407027 Contribution of CD3 gamma to TCR regulation and signaling in... | ACCEPT | Summary: CD3G contributes to T cell activation through the TCR-CD3 receptor. Reason: The human study reports specialized activation responses despite residual signaling. Retain the broad activation process without treating CD3G as independently sufficient to activate a T cell. Supporting Evidence: |
| GO:0042608 T cell receptor binding | NAS PMID:11186279 Fourteen years' follow-up of an autoimmune patient lacking t... | KEEP AS NON CORE | Summary: The TCR association is retained, with the original letter-specific evidence unresolved. Reason: The cached PMID:11186279 letter contains no scientific abstract or accessible assay description. Independent human CD3G mutational and receptor-structure evidence establishes the same TCR association; this supports qualified non-core binding without asserting that the original letter directly measured it. |
| GO:0042802 identical protein binding | IDA PMID:14967045 Homooligomerization of the cytoplasmic domain of the T cell ... | KEEP AS NON CORE | Summary: The isolated CD3G cytoplasmic domain oligomerizes in solution. Reason: The complete abstract explicitly includes CD3 gamma among the recombinant cytoplasmic ITAM domains. Retain that in-vitro binding observation without converting it into a native full-length CD3G homodimer or a second physiological receptor architecture. Supporting Evidence: |
| GO:0045059 positive thymic T cell selection | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: CD3G contributes to thymic selection in the context of receptor signaling. Reason: The curated phylogenetic assertion is compatible with TCR-dependent selection and human repertoire abnormalities. Keep the developmental outcome outside the molecular core. The original PAINT IBD and its complete donor experiments were not independently reconstructed. |
| GO:0046629 gamma-delta T cell activation | NAS PMID:30976362 T cell receptor signaling for Ξ³Ξ΄T cell development. | ACCEPT | Summary: The CD3G signaling module participates in gamma-delta T cell activation. Reason: The cited review and independent human reconstituted receptor/activation evidence support the conserved signaling role. This does not mean that CD3G determines gamma-delta antigen specificity. |
| GO:0046631 alpha-beta T cell activation | NAS PMID:29789755 Regulatory mechanisms in T cell receptor signalling. | ACCEPT | Summary: The CD3G signaling module participates in alpha-beta T cell activation. Reason: The review attribution agrees with target human receptor assembly and deficiency evidence. CD3G supports the receptor signal rather than recognizing peptide-MHC independently. |
| GO:0050852 T cell receptor signaling pathway | IDA PMID:1323144 Activation-induced ubiquitination of the T cell antigen rece... | ACCEPT | Summary: CD3G participates in TCR signaling as a receptor subunit with an ITAM. Reason: The original ubiquitination abstract names zeta and delta, so it is not quoted as a gamma-specific ubiquitination assay. Human CD3G-tail binding and deficiency evidence independently support the signaling process; the original source-specific assay remains unverified. |
| GO:0050852 T cell receptor signaling pathway | NAS PMID:29789755 Regulatory mechanisms in T cell receptor signalling. | ACCEPT | Summary: CD3G contributes to TCR signal transmission in adaptive T cells. Reason: This author-statement review is consistent with the independently established human receptor-adaptor and ITAM roles. |
| GO:0050852 T cell receptor signaling pathway | NAS PMID:30976362 T cell receptor signaling for Ξ³Ξ΄T cell development. | ACCEPT | Summary: CD3G supports gamma-delta TCR signal transmission. Reason: The review attribution is consistent with human receptor composition and functional reconstitution; species-specific stoichiometry is not collapsed. |
| GO:0065003 protein-containing complex assembly | NAS PMID:12794121 TCR dynamics in human mature T lymphocytes lacking CD3 gamma... | ACCEPT | Summary: CD3G makes physical contacts that support assembly of the receptor complex. Reason: Human site-directed mutagenesis and structural interfaces identify work done by CD3G in assembly. Residual surface receptors in deficient mature T cells do not erase that contribution, but rule out an absolute requirement in every setting. |
| GO:0070228 regulation of lymphocyte apoptotic process | IMP PMID:12407027 Contribution of CD3 gamma to TCR regulation and signaling in... | KEEP AS NON CORE | Summary: CD3G deficiency alters activation-induced death in human T cells. Reason: The source abstract directly reports increased activation-induced cell death. Retain the downstream cellular outcome as non-core without assigning intrinsic apoptosis-enzyme activity or a universal death phenotype to CD3G. Supporting Evidence: |
| GO:0035612 AP-2 adaptor complex binding | IPI PMID:9230070 Regulation and function of the CD3gamma DxxxLL motif: a bind... | NEW | Summary: The CD3 gamma cytoplasmic DxxxLL motif associates with AP-2 adaptor complexes. Reason: PMID:9230070 recovered AP-2 adaptins from human Jurkat cytosol with immobilized human CD3 gamma tail peptides. Replacing the dileucines with alanines abolished alpha- and beta- adaptin recovery; D127 substitution and altered D-to-LL spacing reduced binding. This supports motif-dependent AP-2 complex association with IPI evidence, without a purified binary interaction or identification of the contacting subunit; the authors could not exclude mediation by another molecule. PMID:8187769 independently links the CD3 gamma dileucine motif to PKC-induced down-regulation of the intact receptor in human JGN transfectants, providing functional context rather than a second AP-2-binding assay. AP-1 was also captured in the peptide assay. AP-2 is selected for the surface-receptor internalization role; AP-1 motif recognition is acknowledged without asserting an additional TGN-trafficking function. Supporting Evidence: PMID:9230070 the CD3gamma D127xxxLL131/132 sequence represents one united motif for binding of both AP-1 and AP-2 |
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Download this section (compressed HTML)Q: How much do CD3G-dependent assembly, ITAM signaling and regulated trafficking separately explain the variable immunodeficiency and regulatory-T-cell phenotypes in patients?
Q: How closely do the CD3 dimer composition and stoichiometry of endogenous human gamma-delta TCR subsets match the reconstituted Vgamma9Vdelta2 and Vgamma5Vdelta1 structures?
Q: Should the preserved enables qualifiers on the four transmembrane signaling receptor activity annotations be curated as contributes_to for CD3 gamma, given its assembly and ITAM signaling role within the antigen-recognizing TCR complex?
Experiment: Compare wild-type CD3G with separate ITAM, phosphoserine and dileucine-motif mutants in a matched human T-cell background, measuring receptor assembly, surface abundance, ligand-triggered internalization and recycling alongside signaling. Report precursor versus mature-chain numbering and receptor-expression controls.
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