CDK7 is a CMGC-family serine/threonine protein kinase that, with cyclin H and the RING-finger assembly factor MAT1, forms the trimeric CDK-activating kinase (CAK). In this complex it phosphorylates the activation-loop (T-loop) threonine of cell-cycle CDKs, including CDK1, CDK2, CDK4 and CDK6, and is the principal CAK of metazoan cells, required to establish CDK1/CDK2 activity and to maintain CDK4/CDK6 activity during G1. The same trimer is the TFIIK kinase module of the general transcription factor TFIIH, where CDK7 phosphorylates Ser5 and Ser7 of the RNA polymerase II CTD heptad repeats at promoters during initiation and promoter escape, promoting capping-enzyme recruitment, promoter-proximal pausing and, through T-loop phosphorylation of CDK9 and phosphorylation of SPT5, the transition to productive elongation. Other substrates include p53 (Ser33). CDK7 is predominantly nuclear, with a minor cytoplasmic pool. It is a conserved single-copy ortholog across eukaryotes (Kin28 in budding yeast, Mcs6 in fission yeast).
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0000307 cyclin-dependent protein kinase holoenzyme complex | IDA PMID:23622515 A Cdk7-Cdk4 T-loop phosphorylation cascade promotes G1 progr... | ACCEPT | Summary: ComplexPortal annotation of CDK7 to the cyclin-dependent kinase holoenzyme (the CDK7-cyclin H-MAT1 CAK trimer). Reason: CDK7 is the catalytic subunit of the trimeric CAK, a cyclin-CDK holoenzyme; the cited paper states this directly and uses the trimer as the physiological CAK for CDK4/6. Supporting Evidence: PMID:23622515 The sole CAK identified in metazoans is itself a CDK, Cdk7, which forms trimeric complexes with cyclin H and the RING-finger protein Mat1 file:human/CDK7/CDK7-uniprot.txt Catalytic subunit of the CDK-activating kinase (CAK) complex, a master |
| GO:0000439 transcription factor TFIIH core complex | IDA PMID:8692841 Isolation and characterization of two human transcription fa... | MODIFY | Summary: CDK7 annotated to the TFIIH core complex based on fractionation of TFIIH-related complexes. Reason: The cited abstract shows the opposite: the core TFIIH* subcomplex is devoid of CAK, whereas CDK7 co-purifies with XPD in ERCC2/CAK, which joins TFIIH* to form the holoenzyme. CDK7 belongs to the CAK/TFIIK module of holo-TFIIH, not to core TFIIH. The location claim is better expressed by the holo complex and CAK-ERCC2 complex terms, both of which the gene already carries. Proposed replacements: transcription factor TFIIH holo complex CAK-ERCC2 complex Supporting Evidence: PMID:8692841 TFIIH* consists of a subset of the TFIIH complex proteins including ERCC3 (XPB), p62, p44, p41, and p34 but is devoid of detectable levels of ERCC2 (XPD) and CAK PMID:8692841 ERCC2/CAK was isolated as a complex that exhibits CAK activity that cosediments with the three CAK components (cdk7, cyclin H, and p36/MAT1) as well as the ERCC2 (XPD) protein |
| GO:0001650 fibrillar center | IDA GO_REF:0000052 | KEEP AS NON CORE | Summary: Human Protein Atlas immunofluorescence places CDK7 in nucleolar fibrillar centers. Reason: Plausible because TFIIH also functions in RNA polymerase I transcription (Reactome lists CDK7-containing TFIIH in Pol I promoter-escape and elongation events), but this is a minor, antibody-based localization and peripheral to the core nucleoplasmic roles. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, |
| GO:0004672 protein kinase activity | IEA GO_REF:0000120 | MODIFY | Summary: Electronic protein kinase activity for CDK7. Reason: Correct but less specific than the evidence: CDK7 is a cyclin-dependent protein kinase that phosphorylates CDK T-loops and the Pol II CTD. Replace with the specific activities already carried by the gene. Proposed replacements: cyclin-dependent protein serine/threonine kinase activity RNA polymerase II CTD heptapeptide repeat kinase activity Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt Catalytic subunit of the CDK-activating kinase (CAK) complex, a master file:human/CDK7/CDK7-uniprot.txt II by mediating phosphorylation of POLR2A at 'Ser-5' of the repetitive |
| GO:0004672 protein kinase activity | TAS PMID:7533895 Cdk-activating kinase complex is a component of human transc... | MODIFY | Summary: Protein kinase activity based on the identification of CAK as the TFIIH CTD kinase. Reason: The paper shows CTD phosphorylation by Cdk7-cyclin H in the preinitiation complex and Cdc2/Cdk2 phosphorylation by TFIIH, which support the more specific CTD kinase and cyclin-dependent kinase terms. Proposed replacements: cyclin-dependent protein serine/threonine kinase activity RNA polymerase II CTD heptapeptide repeat kinase activity Supporting Evidence: PMID:7533895 both cyclin H and Cdk7 antibodies inhibit phosphorylation of the CTD of the largest subunit of the RNAPII in the preinitiation complex PMID:7533895 TFIIH was shown to phosphorylate both Cdc2 and Cdk2 |
| GO:0004674 protein serine/threonine kinase activity | IEA GO_REF:0000107 | MODIFY | Summary: Electronic (ortholog-transfer) protein serine/threonine kinase activity. Reason: True but general; CDK7's serine/threonine kinase activity is specifically cyclin-dependent and directed at CDK T-loops and the Pol II CTD. Proposed replacements: cyclin-dependent protein serine/threonine kinase activity RNA polymerase II CTD heptapeptide repeat kinase activity Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt Catalytic subunit of the CDK-activating kinase (CAK) complex, a master file:human/CDK7/CDK7-uniprot.txt II by mediating phosphorylation of POLR2A at 'Ser-5' of the repetitive |
| GO:0004674 protein serine/threonine kinase activity | TAS Reactome:R-HSA-6810233 | MODIFY | Summary: Reactome reaction in which CDK7 phosphorylates CTD Ser5 and Ser7 at snRNA promoters. Reason: The reaction describes CTD Ser5 and Ser7 kinase activity, for which specific GO terms exist. Proposed replacements: RNA polymerase II CTD heptapeptide repeat S5 kinase activity RNA polymerase II CTD heptapeptide repeat S7 kinase activity Supporting Evidence: Reactome:R-HSA-6810233 CDK7 phosphorylates serine-5 residues of heptad repeats (consensus YSPTSPS) in the C-terminal domain (CTD) of the large subunit (POLR2A) of RNA polymerase II Reactome:R-HSA-6810233 CDK7 is required for phosphorylation of serine-7 in vivo |
| GO:0004674 protein serine/threonine kinase activity | TAS Reactome:R-HSA-77071 | MODIFY | Summary: Reactome reaction 'Phosphorylation (Ser5) of RNA pol II CTD'. Reason: The reaction is Ser5 CTD phosphorylation, which maps to the specific CTD S5 kinase term. Proposed replacements: RNA polymerase II CTD heptapeptide repeat S5 kinase activity Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt II by mediating phosphorylation of POLR2A at 'Ser-5' of the repetitive PMID:26257281 Cdk7 phosphorylates Ser5 and Ser7 residues, the first of several CTD phosphorylation and dephosphorylation events during the transcription cycle |
| GO:0004693 cyclin-dependent protein serine/threonine kinase activity | IBA GO_REF:0000033 | ACCEPT | Summary: Phylogenetic inference of cyclin-dependent protein serine/threonine kinase activity. Reason: The IBA derives from PAINT node PANTHER:PTN000624096, the eukaryote-wide CDK7 ortholog clade of PTHR24056 (members include S. cerevisiae Kin28, S. pombe Mcs6 [SPBC19F8.07], Drosophila Cdk7 and rat Cdk7); human CDK7 sits inside this clade (UniProt PTHR24056:SF0) and has its own experimental support, so its appearance among the donors is expected, not circular. CDK7 requires cyclin H (and MAT1) for activity and phosphorylates CDK T-loops, matching the term. Supporting Evidence: PMID:15530371 For maximum activity and stability, CDK7 requires phosphorylation, association with cyclin H, and association with a third protein, MAT1 PMID:23622515 Here we analyze activation of Cdk4 and Cdk6 in Cdk7as cells, and show that Cdk7 is a physiologic CAK for both cyclin D-dependent kinases |
| GO:0004693 cyclin-dependent protein serine/threonine kinase activity | IEA GO_REF:0000003 | ACCEPT | Summary: EC-mapping-based cyclin-dependent kinase activity. Reason: Consistent with the experimental CAK activity of CDK7-cyclin H-MAT1. Supporting Evidence: PMID:15530371 For maximum activity and stability, CDK7 requires phosphorylation, association with cyclin H, and association with a third protein, MAT1 file:human/CDK7/CDK7-uniprot.txt mediators of cell cycle control, including CDK1, CDK2, CDK4 and CDK6, |
| GO:0004693 cyclin-dependent protein serine/threonine kinase activity | TAS PMID:8208544 Two novel human serine/threonine kinases with homologies to ... | ACCEPT | Summary: Cyclin-dependent kinase activity traced to the cloning of human MO15/STK1. Reason: The cloning paper itself infers function from 86% identity to Xenopus MO15, the known CAK; the activity is independently established for human CDK7, so the term stands. Supporting Evidence: PMID:8208544 STK1 is 86% identical to the Xenopus p40mo15, a cdc2-related serine/threonine kinase recently found to be the activating kinase for p34cdc2 and p33cdk2 PMID:23622515 Here we analyze activation of Cdk4 and Cdk6 in Cdk7as cells, and show that Cdk7 is a physiologic CAK for both cyclin D-dependent kinases |
| GO:0005515 protein binding | IPI PMID:10801852 Distinct regions of MAT1 regulate cdk7 kinase and TFIIH tran... | REMOVE | Summary: Protein binding (IPI) with MAT1 (MNAT1). Reason: MAT1 is a CAK subunit; the association is captured by the TFIIK/CAK complex annotations. Bare protein binding is uninformative, so under the repository protein-binding policy the row is removed; this does not dispute that the interaction was observed. Supporting Evidence: PMID:10801852 the C terminus of MAT1 binds to the cdk7 x cyclin H complex and activates the cdk7 kinase activity |
| GO:0005515 protein binding | IPI PMID:10958787 Interactions of Cdk7 and Kin28 with Hint/PKCI-1 and Hnt1 his... | REMOVE | Summary: Protein binding (IPI) with HINT1. Reason: The HINT1 association has no defined CDK7 activity attached to it. Bare protein binding is uninformative, so under the repository protein-binding policy the row is removed; this does not dispute that the interaction was observed. |
| GO:0005515 protein binding | IPI PMID:15530371 The crystal structure of human CDK7 and its protein recognit... | REMOVE | Summary: Protein binding (IPI) with CDK2. Reason: CDK2 is a CAK substrate; the relationship is captured by the cyclin-dependent kinase activity annotation. Bare protein binding is uninformative, so under the repository protein-binding policy the row is removed; this does not dispute that the interaction was observed. Supporting Evidence: PMID:15530371 For maximum activity and stability, CDK7 requires phosphorylation, association with cyclin H, and association with a third protein, MAT1 |
| GO:0005515 protein binding | IPI PMID:16327805 Dichotomous but stringent substrate selection by the dual-fu... | REMOVE | Summary: Protein binding (IPI) with a substrate (SUPT5H, CDK4 or CDK2) from the analog-sensitive kinase screen. Reason: The partners are substrates, captured by the kinase activity annotations. Bare protein binding is uninformative, so under the repository protein-binding policy the row is removed; this does not dispute that the interaction was observed. Supporting Evidence: PMID:16327805 proteins such as Pol II and transcription elongation factor Spt5, recognized efficiently only by the fully activated Cdk7 complex |
| GO:0005515 protein binding | IPI PMID:16327805 Dichotomous but stringent substrate selection by the dual-fu... | REMOVE | Summary: Protein binding (IPI) with a substrate (SUPT5H, CDK4 or CDK2) from the analog-sensitive kinase screen. Reason: The partners are substrates, captured by the kinase activity annotations. Bare protein binding is uninformative, so under the repository protein-binding policy the row is removed; this does not dispute that the interaction was observed. Supporting Evidence: PMID:16327805 proteins such as Pol II and transcription elongation factor Spt5, recognized efficiently only by the fully activated Cdk7 complex |
| GO:0005515 protein binding | IPI PMID:16327805 Dichotomous but stringent substrate selection by the dual-fu... | REMOVE | Summary: Protein binding (IPI) with a substrate (SUPT5H, CDK4 or CDK2) from the analog-sensitive kinase screen. Reason: The partners are substrates, captured by the kinase activity annotations. Bare protein binding is uninformative, so under the repository protein-binding policy the row is removed; this does not dispute that the interaction was observed. Supporting Evidence: PMID:16327805 proteins such as Pol II and transcription elongation factor Spt5, recognized efficiently only by the fully activated Cdk7 complex |
| GO:0005515 protein binding | IPI PMID:22939624 Quantitative analysis of HSP90-client interactions reveals p... | REMOVE | Summary: Protein binding (IPI) with HSP90AA1. Reason: This is a chaperone-client interaction from a proteome-scale HSP90 study. Bare protein binding is uninformative, so under the repository protein-binding policy the row is removed; this does not dispute that the interaction was observed. |
| GO:0005515 protein binding | IPI PMID:23393140 Interaction with cyclin H/cyclin-dependent kinase 7 (CCNH/CD... | MODIFY | Summary: Protein binding (IPI) with CtBP2 and cyclin H (CCNH). Reason: The interaction set includes cyclin H, the activating cyclin of CDK7; the informative molecular function is cyclin binding. The CtBP2 association is recorded under the protein stabilization row. Proposed replacements: cyclin binding Supporting Evidence: PMID:23393140 the cyclin H/cyclin-dependent kinase 7 (CCNH/CDK7) complex interacted with CtBP2 in vivo and in vitro PMID:15530371 For maximum activity and stability, CDK7 requires phosphorylation, association with cyclin H, and association with a third protein, MAT1 |
| GO:0005515 protein binding | IPI PMID:23455922 Interlaboratory reproducibility of large-scale human protein... | REMOVE | Summary: Protein binding (IPI) with CDK2. Reason: High-throughput AP-MS of kinases; CDK2 is a CAK substrate. Bare protein binding is uninformative, so under the repository protein-binding policy the row is removed; this does not dispute that the interaction was observed. |
| GO:0005515 protein binding | IPI PMID:23602568 The protein interaction landscape of the human CMGC kinase g... | REMOVE | Summary: Protein binding (IPI) with SRPK2 or SRPK1. Reason: High-throughput AP-MS of the CMGC kinase group; no CDK7 function follows from it. Bare protein binding is uninformative, so under the repository protein-binding policy the row is removed; this does not dispute that the interaction was observed. |
| GO:0005515 protein binding | IPI PMID:23602568 The protein interaction landscape of the human CMGC kinase g... | REMOVE | Summary: Protein binding (IPI) with SRPK2 or SRPK1. Reason: High-throughput AP-MS of the CMGC kinase group; no CDK7 function follows from it. Bare protein binding is uninformative, so under the repository protein-binding policy the row is removed; this does not dispute that the interaction was observed. |
| GO:0005515 protein binding | IPI PMID:28514442 Architecture of the human interactome defines protein commun... | REMOVE | Summary: Protein binding (IPI) with HSP90AA1. Reason: Proteome-scale interactome data (BioPlex). Bare protein binding is uninformative, so under the repository protein-binding policy the row is removed; this does not dispute that the interaction was observed. |
| GO:0005515 protein binding | IPI PMID:32707033 Kinase Interaction Network Expands Functional and Disease Ro... | REMOVE | Summary: Protein binding (IPI) with SRPK2 or SRPK1. Reason: Proteome-scale kinase interaction network. Bare protein binding is uninformative, so under the repository protein-binding policy the row is removed; this does not dispute that the interaction was observed. |
| GO:0005515 protein binding | IPI PMID:32707033 Kinase Interaction Network Expands Functional and Disease Ro... | REMOVE | Summary: Protein binding (IPI) with SRPK2 or SRPK1. Reason: Proteome-scale kinase interaction network. Bare protein binding is uninformative, so under the repository protein-binding policy the row is removed; this does not dispute that the interaction was observed. |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | REMOVE | Summary: Protein binding (IPI) with HSP90AA1. Reason: Proteome-scale interactome data. Bare protein binding is uninformative, so under the repository protein-binding policy the row is removed; this does not dispute that the interaction was observed. |
| GO:0005524 ATP binding | IEA GO_REF:0000002 | ACCEPT | Summary: ATP binding inferred from the protein kinase domain. Reason: CDK7 is an active protein kinase; the crystal structure was solved with bound ATP and UniProt maps the ATP-binding residues in the glycine-rich loop. Supporting Evidence: PMID:15530371 We have determined the crystal structure of human CDK7 in complex with ATP at 3 A resolution |
| GO:0005634 nucleus | EXP PMID:15695176 Cyclin-dependent kinase activating kinase/Cdk7 co-localizes ... | ACCEPT | Summary: Nuclear localization by confocal co-localization with PKC-iota in glioma cells. Reason: CDK7 functions in the nucleus as part of TFIIH and the CAK complex; the C-terminal KRKR motif targets it there. Supporting Evidence: PMID:15695176 Confocal microscopy revealed the co-localization of PKC-iota with CAK/cdk7 in both the cytoplasm and nucleus of U-373 MG cells PMID:7936635 the wild-type protein is detected exclusively in the nucleus |
| GO:0005634 nucleus | EXP PMID:19071173 DNA-Bound peptides control the mRNA transcription through CD... | ACCEPT | Summary: Nuclear localization reported in a study of barley DNA-bound peptides acting via CDK7. Reason: The paper's main claim (peptide-induced translocation) is weak and condition-specific, but the nuclear location of CDK7 itself is well established, so the location annotation stands. Supporting Evidence: PMID:19071173 The DNA-Bound peptides also significantly inhibited in vitro and in vivo DNA transcription activity by regulating the expression and the corresponding functions of CDK7 PMID:7936635 the wild-type protein is detected exclusively in the nucleus |
| GO:0005634 nucleus | IBA GO_REF:0000033 | ACCEPT | Summary: Phylogenetic inference that CDK7 is active in the nucleus. Reason: Nuclear activity is inferred from a deep CDK node (PTN000623091) and is consistent with direct evidence for human CDK7. Supporting Evidence: PMID:7936635 the wild-type protein is detected exclusively in the nucleus |
| GO:0005634 nucleus | IDA PMID:10958787 Interactions of Cdk7 and Kin28 with Hint/PKCI-1 and Hnt1 his... | ACCEPT | Summary: Nuclear localization of CDK7 (Hint partner study). Reason: CDK7 functions in the nucleus as part of TFIIH and the CAK complex; the C-terminal KRKR motif targets it there. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7936635 the wild-type protein is detected exclusively in the nucleus |
| GO:0005634 nucleus | IDA PMID:16109376 The bromodomain protein Brd4 is a positive regulatory compon... | ACCEPT | Summary: Nuclear localization of CDK7 (MGI-curated, Brd4/P-TEFb study). Reason: CDK7 functions in the nucleus as part of TFIIH and the CAK complex; the C-terminal KRKR motif targets it there. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7936635 the wild-type protein is detected exclusively in the nucleus |
| GO:0005634 nucleus | IEA GO_REF:0000120 | ACCEPT | Summary: Electronic nucleus annotation. Reason: CDK7 functions in the nucleus as part of TFIIH and the CAK complex; the C-terminal KRKR motif targets it there. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, |
| GO:0005634 nucleus | TAS PMID:7936635 Cloning, expression and subcellular localization of the huma... | ACCEPT | Summary: Nuclear localization of tagged human MO15/CDK7. Reason: Wild-type CDK7 is detected exclusively in the nucleus and nuclear targeting is required for CAK activity. Supporting Evidence: PMID:7936635 the wild-type protein is detected exclusively in the nucleus |
| GO:0005654 nucleoplasm | IDA GO_REF:0000052 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | IEA GO_REF:0000107 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-109639 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-111264 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-112379 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-112383 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-112385 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-113430 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-170076 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-170087 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-187949 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-188350 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-5689861 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-5691000 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6781818 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6781824 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6781833 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6781840 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6781867 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6782004 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6782069 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6782131 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6782138 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6782141 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6782204 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6782208 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6782211 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6782224 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6782227 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6782234 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6797606 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6797616 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6810233 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6810234 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6810235 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6810238 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6814549 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6814555 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6814559 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6814885 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-73758 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-73769 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-73946 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-74986 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-74992 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-74993 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-74994 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75850 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75856 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75861 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75862 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75864 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75866 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75869 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75873 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75891 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75949 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-76576 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77068 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77069 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77071 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77073 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77077 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77078 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77081 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77083 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77085 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77090 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-8942836 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9613494 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9613497 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9684118 | ACCEPT | Summary: CDK7 located in the nucleoplasm. Reason: CDK7 acts in the nucleoplasm as the TFIIH/TFIIK kinase on promoter-bound RNA polymerase II and as the CAK. The many Reactome reactions place TFIIH (and therefore CDK7) in the nucleoplasm across Pol II, snRNA, Pol I and TC-NER events; the location is correct irrespective of whether CDK7 is catalytic in each individual reaction. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10958787, PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005675 transcription factor TFIIH holo complex | IDA PMID:9852112 Immunoaffinity purification and functional characterization ... | ACCEPT | Summary: CDK7 in the TFIIH holo complex (immunopurified FLAG-TFIIH). Reason: CAK (CDK7-cyclin H-MAT1) is the kinase module of holo-TFIIH. Supporting Evidence: PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography PMID:9852112 The purified complexes are functional in mediating basal and activated transcription |
| GO:0005675 transcription factor TFIIH holo complex | IEA GO_REF:0000002 | ACCEPT | Summary: Electronic TFIIH holo complex annotation. Reason: CDK7 is a stoichiometric TFIIH component via the CAK module. Supporting Evidence: PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005675 transcription factor TFIIH holo complex | IPI PMID:15220921 A new, tenth subunit of TFIIH is responsible for the DNA rep... | ACCEPT | Summary: ComplexPortal TFIIH holo complex membership. Reason: Consistent with purification of CAK within TFIIH. Supporting Evidence: PMID:15220921 Here, we describe the identification of the human TFB5 ortholog and its association with human TFIIH PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography |
| GO:0005737 cytoplasm | EXP PMID:15695176 Cyclin-dependent kinase activating kinase/Cdk7 co-localizes ... | KEEP AS NON CORE | Summary: Cytoplasmic CDK7 in glioma cells co-localizing with PKC-iota. Reason: A fraction of CDK7 is cytoplasmic (free CAK); the functional pool is nuclear, so this is a minor location. Supporting Evidence: PMID:15695176 Confocal microscopy revealed the co-localization of PKC-iota with CAK/cdk7 in both the cytoplasm and nucleus of U-373 MG cells |
| GO:0005737 cytoplasm | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: Phylogenetic inference of cytoplasmic activity. Reason: The IBA derives from PAINT node PANTHER:PTN000624096, the eukaryote-wide CDK7 ortholog clade of PTHR24056 (members include S. cerevisiae Kin28, S. pombe Mcs6 [SPBC19F8.07], Drosophila Cdk7 and rat Cdk7); human CDK7 sits inside this clade (UniProt PTHR24056:SF0) and has its own experimental support, so its appearance among the donors is expected, not circular. A fraction of CDK7 is cytoplasmic (free CAK); the functional pool is nuclear, so this is a minor location. Supporting Evidence: PMID:15695176 Confocal microscopy revealed the co-localization of PKC-iota with CAK/cdk7 in both the cytoplasm and nucleus of U-373 MG cells |
| GO:0005737 cytoplasm | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: Cytoplasm from UniProt subcellular location mapping. Reason: A fraction of CDK7 is cytoplasmic (free CAK); the functional pool is nuclear, so this is a minor location. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt ECO:0000269|PubMed:15695176, ECO:0000269|PubMed:19071173}. Cytoplasm |
| GO:0005829 cytosol | IDA GO_REF:0000052 | KEEP AS NON CORE | Summary: HPA immunofluorescence cytosol signal. Reason: A fraction of CDK7 is cytoplasmic (free CAK); the functional pool is nuclear, so this is a minor location. Supporting Evidence: PMID:15695176 Confocal microscopy revealed the co-localization of PKC-iota with CAK/cdk7 in both the cytoplasm and nucleus of U-373 MG cells |
| GO:0006289 nucleotide-excision repair | NAS PMID:22572993 TFIIH: when transcription met DNA repair. | MARK AS OVER ANNOTATED | Summary: Nucleotide-excision repair attributed to CDK7 from a TFIIH review. Reason: TFIIH participates in NER through its core helicases XPB/XPD, but the CAK subcomplex is released from TFIIH when XPA binds the lesion (Reactome R-HSA-5689861), and the cited review is about TFIIH as a whole. CDK7 does not perform an NER step; the annotation transfers a complex-level process to the kinase module. Supporting Evidence: PMID:22572993 The transcription initiation factor TFIIH is a remarkable protein complex that has a fundamental role in the transcription of protein-coding genes as well as during the DNA nucleotide excision repair pathway Reactome:R-HSA-5689861 Binding of XPA is accompanied by the release of the CAK subcomplex from the TFIIH complex |
| GO:0006366 transcription by RNA polymerase II | TAS Reactome:R-HSA-73857 | MODIFY | Summary: Reactome 'RNA Polymerase II Transcription' for CDK7. Reason: Correct but general. CDK7's direct role is CTD Ser5/Ser7 phosphorylation at promoters during initiation and promoter escape; the specific process term is already carried by the gene. Proposed replacements: transcription initiation at RNA polymerase II promoter Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt II by mediating phosphorylation of POLR2A at 'Ser-5' of the repetitive PMID:26257281 Inhibition of Cdk7 blocks Pol II CTD phosphorylation during initiation |
| GO:0006367 transcription initiation at RNA polymerase II promoter | IBA GO_REF:0000033 | ACCEPT | Summary: Phylogenetic inference of Pol II transcription initiation. Reason: The IBA derives from PAINT node PANTHER:PTN000624096, the eukaryote-wide CDK7 ortholog clade of PTHR24056 (members include S. cerevisiae Kin28, S. pombe Mcs6 [SPBC19F8.07], Drosophila Cdk7 and rat Cdk7); human CDK7 sits inside this clade (UniProt PTHR24056:SF0) and has its own experimental support, so its appearance among the donors is expected, not circular. CDK7, as the TFIIH kinase, phosphorylates the CTD of promoter-bound Pol II during initiation; THZ1 inhibition blocks this initiation-associated phosphorylation. Supporting Evidence: PMID:26257281 Inhibition of Cdk7 blocks Pol II CTD phosphorylation during initiation |
| GO:0006367 transcription initiation at RNA polymerase II promoter | IDA PMID:26257281 THZ1 Reveals Roles for Cdk7 in Co-transcriptional Capping an... | ACCEPT | Summary: Transcription initiation: THZ1 inhibition of CDK7 blocks CTD phosphorylation during initiation. Reason: CDK7, as the TFIIH kinase, phosphorylates the CTD of promoter-bound Pol II during initiation; THZ1 inhibition blocks this initiation-associated phosphorylation. Supporting Evidence: PMID:26257281 Inhibition of Cdk7 blocks Pol II CTD phosphorylation during initiation |
| GO:0006367 transcription initiation at RNA polymerase II promoter | IDA PMID:7533895 Cdk-activating kinase complex is a component of human transc... | ACCEPT | Summary: Transcription initiation: anti-CDK7 antibodies block CTD phosphorylation in the preinitiation complex. Reason: CDK7, as the TFIIH kinase, phosphorylates the CTD of promoter-bound Pol II during initiation; THZ1 inhibition blocks this initiation-associated phosphorylation. Supporting Evidence: PMID:7533895 both cyclin H and Cdk7 antibodies inhibit phosphorylation of the CTD of the largest subunit of the RNAPII in the preinitiation complex |
| GO:0006367 transcription initiation at RNA polymerase II promoter | IDA PMID:8692841 Isolation and characterization of two human transcription fa... | ACCEPT | Summary: Transcription initiation: ERCC2/CAK stimulates TFIIH*-dependent transcription. Reason: CDK7, as the TFIIH kinase, phosphorylates the CTD of promoter-bound Pol II during initiation; THZ1 inhibition blocks this initiation-associated phosphorylation. Supporting Evidence: PMID:8692841 This TFIIH*-dependent transcription reaction was stimulated by ERCC2/CAK |
| GO:0006367 transcription initiation at RNA polymerase II promoter | NAS PMID:22572993 TFIIH: when transcription met DNA repair. | ACCEPT | Summary: Transcription initiation (TFIIH review). Reason: CDK7, as the TFIIH kinase, phosphorylates the CTD of promoter-bound Pol II during initiation; THZ1 inhibition blocks this initiation-associated phosphorylation. Supporting Evidence: PMID:22572993 The transcription initiation factor TFIIH is a remarkable protein complex that has a fundamental role in the transcription of protein-coding genes as well as during the DNA nucleotide excision repair pathway PMID:26257281 Inhibition of Cdk7 blocks Pol II CTD phosphorylation during initiation |
| GO:0006367 transcription initiation at RNA polymerase II promoter | TAS PMID:7533895 Cdk-activating kinase complex is a component of human transc... | ACCEPT | Summary: Transcription initiation (TAS, CAK in TFIIH). Reason: CDK7, as the TFIIH kinase, phosphorylates the CTD of promoter-bound Pol II during initiation; THZ1 inhibition blocks this initiation-associated phosphorylation. Supporting Evidence: PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography PMID:7533895 both cyclin H and Cdk7 antibodies inhibit phosphorylation of the CTD of the largest subunit of the RNAPII in the preinitiation complex |
| GO:0008094 ATP-dependent activity, acting on DNA | IDA PMID:9852112 Immunoaffinity purification and functional characterization ... | REMOVE | Summary: ATP-dependent activity acting on DNA, from functional characterization of immunopurified TFIIH. Reason: CDK7 is a protein kinase (single kinase domain 12-295, no helicase or DNA-translocase motifs); the DNA-dependent ATPase/helicase activities of TFIIH reside in XPB (ERCC3) and XPD (ERCC2). The cited abstract characterizes the purified TFIIH complex, and the activity was attributed to every subunit. This is a complex-activity transfer to a non-catalytic subunit for this activity. Supporting Evidence: PMID:9852112 The purified complexes are functional in mediating basal and activated transcription PMID:8692841 TFIIH* consists of a subset of the TFIIH complex proteins including ERCC3 (XPB), p62, p44, p41, and p34 but is devoid of detectable levels of ERCC2 (XPD) and CAK |
| GO:0008353 RNA polymerase II CTD heptapeptide repeat kinase activity | EXP PMID:26257281 THZ1 Reveals Roles for Cdk7 in Co-transcriptional Capping an... | ACCEPT | Summary: CTD kinase activity (THZ1 study). Reason: CDK7 is the TFIIH CTD kinase; chemical-genetic and THZ1 inhibition abolish initiation-associated CTD Ser5/Ser7 phosphorylation. Supporting Evidence: PMID:26257281 Inhibition of Cdk7 blocks Pol II CTD phosphorylation during initiation PMID:26257281 Cdk7 phosphorylates Ser5 and Ser7 residues, the first of several CTD phosphorylation and dephosphorylation events during the transcription cycle |
| GO:0008353 RNA polymerase II CTD heptapeptide repeat kinase activity | EXP PMID:28768201 Human TFIIH Kinase CDK7 Regulates Transcription-Associated C... | ACCEPT | Summary: CTD kinase activity (CDK7as cells). Reason: CDK7 is the TFIIH CTD kinase; chemical-genetic and THZ1 inhibition abolish initiation-associated CTD Ser5/Ser7 phosphorylation. Supporting Evidence: PMID:28768201 During transcription initiation, CDK7 phosphorylates the C-terminal domain (CTD) of the pol II subunit RPB1. PMID:28768201 CDK7 phosphorylates the CTD on Ser5 and Ser7 |
| GO:0008353 RNA polymerase II CTD heptapeptide repeat kinase activity | IBA GO_REF:0000033 | ACCEPT | Summary: Phylogenetic inference of CTD kinase activity. Reason: The IBA derives from PAINT node PANTHER:PTN000624096, the eukaryote-wide CDK7 ortholog clade of PTHR24056 (members include S. cerevisiae Kin28, S. pombe Mcs6 [SPBC19F8.07], Drosophila Cdk7 and rat Cdk7); human CDK7 sits inside this clade (UniProt PTHR24056:SF0) and has its own experimental support, so its appearance among the donors is expected, not circular. CDK7 is the TFIIH CTD kinase; chemical-genetic and THZ1 inhibition abolish initiation-associated CTD Ser5/Ser7 phosphorylation. Supporting Evidence: PMID:28768201 During transcription initiation, CDK7 phosphorylates the C-terminal domain (CTD) of the pol II subunit RPB1. |
| GO:0008353 RNA polymerase II CTD heptapeptide repeat kinase activity | IDA PMID:12721286 A novel RNA polymerase II C-terminal domain phosphatase that... | ACCEPT | Summary: CTD kinase activity: TFIIH-phosphorylated Pol II used as substrate for SCP1. Reason: TFIIH (via CDK7) phosphorylates CTD Ser5, generating the preferred SCP1 substrate. Supporting Evidence: PMID:12721286 The preferred substrate for SCP1 is RNAP II phosphorylated by TFIIH file:human/CDK7/CDK7-uniprot.txt II by mediating phosphorylation of POLR2A at 'Ser-5' of the repetitive |
| GO:0008353 RNA polymerase II CTD heptapeptide repeat kinase activity | IDA PMID:9852112 Immunoaffinity purification and functional characterization ... | ACCEPT | Summary: CTD kinase activity of immunopurified TFIIH. Reason: CDK7 is the TFIIH CTD kinase; chemical-genetic and THZ1 inhibition abolish initiation-associated CTD Ser5/Ser7 phosphorylation. Supporting Evidence: PMID:7533895 both cyclin H and Cdk7 antibodies inhibit phosphorylation of the CTD of the largest subunit of the RNAPII in the preinitiation complex file:human/CDK7/CDK7-uniprot.txt II by mediating phosphorylation of POLR2A at 'Ser-5' of the repetitive |
| GO:0008353 RNA polymerase II CTD heptapeptide repeat kinase activity | IEA GO_REF:0000120 | ACCEPT | Summary: Electronic CTD kinase activity. Reason: CDK7 is the TFIIH CTD kinase; chemical-genetic and THZ1 inhibition abolish initiation-associated CTD Ser5/Ser7 phosphorylation. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt II by mediating phosphorylation of POLR2A at 'Ser-5' of the repetitive |
| GO:0016301 kinase activity | IEA GO_REF:0000107 | MODIFY | Summary: Electronic kinase activity. Reason: Too general; CDK7 is a cyclin-dependent protein kinase acting on CDKs and the Pol II CTD. Proposed replacements: cyclin-dependent protein serine/threonine kinase activity RNA polymerase II CTD heptapeptide repeat kinase activity Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt Catalytic subunit of the CDK-activating kinase (CAK) complex, a master file:human/CDK7/CDK7-uniprot.txt II by mediating phosphorylation of POLR2A at 'Ser-5' of the repetitive |
| GO:0042795 snRNA transcription by RNA polymerase II | TAS Reactome:R-HSA-6807505 | KEEP AS NON CORE | Summary: Reactome snRNA transcription by RNA polymerase II. Reason: CDK7/TFIIH phosphorylates CTD Ser5 and Ser7 at snRNA promoters, but Reactome notes that loss of CTD phosphorylation impairs pre-snRNA 3' processing rather than snRNA transcription itself. A valid but gene-class-specific context of the core initiation role. Supporting Evidence: Reactome:R-HSA-6810233 CDK7 phosphorylates serine-5 residues of heptad repeats (consensus YSPTSPS) in the C-terminal domain (CTD) of the large subunit (POLR2A) of RNA polymerase II Reactome:R-HSA-6810233 Impairment of CTD phosphorylation does not appear to affect transcription of snRNA genes but rather impairs 3' processing of the pre-snRNA |
| GO:0045944 positive regulation of transcription by RNA polymerase II | IDA PMID:9852112 Immunoaffinity purification and functional characterization ... | MARK AS OVER ANNOTATED | Summary: Positive regulation of Pol II transcription from purified TFIIH supporting basal and activated transcription. Reason: The cited work shows that purified TFIIH complexes support basal and activated transcription; that is participation of a general transcription factor in transcription initiation (already annotated), not a CDK7-specific regulatory activity at particular genes. Supporting Evidence: PMID:9852112 The purified complexes are functional in mediating basal and activated transcription |
| GO:0048471 perinuclear region of cytoplasm | IEA GO_REF:0000044 | MARK AS OVER ANNOTATED | Summary: Perinuclear region from UniProt subcellular location. Reason: The source observation is relocation of CDK7 after treatment of HeLa cells with barley DNA-bound peptides, a non-physiological condition in a single study; it does not describe where CDK7 normally acts. Supporting Evidence: file:human/CDK7/CDK7-uniprot.txt to cytoplasm and perinuclear region in response to DNA-bound peptides PMID:19071173 The DNA-Bound peptides also significantly inhibited in vitro and in vivo DNA transcription activity by regulating the expression and the corresponding functions of CDK7 |
| GO:0050821 protein stabilization | IMP PMID:23393140 Interaction with cyclin H/cyclin-dependent kinase 7 (CCNH/CD... | KEEP AS NON CORE | Summary: Protein stabilization: CCNH/CDK7 binding protects CtBP2 from proteasomal clearance. Reason: CCNH/CDK7 binding competes with HIPK2 and blocks CtBP2 phosphorylation and degradation, so the complex does contribute to CtBP2 stabilization. Single-study, cancer-cell context, and peripheral to the CAK and CTD-kinase roles. Supporting Evidence: PMID:23393140 Depletion of either CCNH or CDK7 decreased CtBP2 protein levels by accelerating proteasome-dependent CtBP2 clearance PMID:23393140 CCNH/CDK7 competed with the tumor repressor HIPK2 for CtBP2 binding and consequently inhibited phosphorylation and dimerization of CtBP2 |
| GO:0051726 regulation of cell cycle | IBA GO_REF:0000033 | MODIFY | Summary: Phylogenetic inference of regulation of cell cycle. Reason: The IBA derives from PAINT node PANTHER:PTN000624096, the eukaryote-wide CDK7 ortholog clade of PTHR24056 (members include S. cerevisiae Kin28, S. pombe Mcs6 [SPBC19F8.07], Drosophila Cdk7 and rat Cdk7); human CDK7 sits inside this clade (UniProt PTHR24056:SF0) and has its own experimental support, so its appearance among the donors is expected, not circular. CDK7 regulates the cell cycle as the CAK, phosphorylating the T-loops of CDK4/6 and CDK2 (G1/S) and CDK1 (G2/M). The general term is true and also entailed by GO:0004693; the evidence supports the specific transitions. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000624096 · PTN000624096 SUPPORTS TRANSFER CDK7 ortholog clade node seeded by human CDK7 and C9K505; regulation of cell cycle is correct for the clade, and the MODIFY only refines it to the G1/S and G2/M transitions supported for human CDK7. Proposed replacements: regulation of G1/S transition of mitotic cell cycle regulation of cell cycle G2/M phase transition Supporting Evidence: PMID:23622515 Here we analyze activation of Cdk4 and Cdk6 in Cdk7as cells, and show that Cdk7 is a physiologic CAK for both cyclin D-dependent kinases PMID:20360007 we find that Cdc25A overexpression leads to an activation of Cdk7 and increase in Thr(161) phosphorylation of Cdk1 |
| GO:0051726 regulation of cell cycle | TAS PMID:15876871 CAK-Cyclin-dependent Activating Kinase: a key kinase in cell... | MODIFY | Summary: Regulation of cell cycle (review of CAK). Reason: CDK7 regulates the cell cycle as the CAK, phosphorylating the T-loops of CDK4/6 and CDK2 (G1/S) and CDK1 (G2/M). The general term is true and also entailed by GO:0004693; the evidence supports the specific transitions. Proposed replacements: regulation of G1/S transition of mitotic cell cycle regulation of cell cycle G2/M phase transition Supporting Evidence: PMID:15876871 The Cyclin-dependent kinase (CDK) Activating Kinase (CAK) is responsible for the activating phosphorylation of CDK1, CDK2, CDK4 and CDK6 and regulation of the cell cycle PMID:23622515 this suggests that Cdk7 is a universal CAK for CDKs involved in human cell division |
| GO:0070516 CAK-ERCC2 complex | IDA PMID:8692841 Isolation and characterization of two human transcription fa... | ACCEPT | Summary: CAK-ERCC2 complex isolated from HeLa cells. Reason: The ERCC2/CAK complex cosediments CDK7, cyclin H, MAT1 and XPD. Supporting Evidence: PMID:8692841 ERCC2/CAK was isolated as a complex that exhibits CAK activity that cosediments with the three CAK components (cdk7, cyclin H, and p36/MAT1) as well as the ERCC2 (XPD) protein |
| GO:0070985 transcription factor TFIIK complex | IBA GO_REF:0000033 | ACCEPT | Summary: Phylogenetic inference of TFIIK complex membership. Reason: The IBA derives from PAINT node PANTHER:PTN000624096, the eukaryote-wide CDK7 ortholog clade of PTHR24056 (members include S. cerevisiae Kin28, S. pombe Mcs6 [SPBC19F8.07], Drosophila Cdk7 and rat Cdk7); human CDK7 sits inside this clade (UniProt PTHR24056:SF0) and has its own experimental support, so its appearance among the donors is expected, not circular. TFIIK is the CDK7-cyclin H-MAT1 kinase module of TFIIH, which also exists free as CAK. Supporting Evidence: PMID:7533895 Here we report the identification of the Cdk-activating kinase (Cak) complex (Cdk7 and cyclin H) as a component of TFIIH after extensive purification of TFIIH by chromatography PMID:10801852 the C terminus of MAT1 binds to the cdk7 x cyclin H complex and activates the cdk7 kinase activity |
| GO:0070985 transcription factor TFIIK complex | IDA PMID:8692841 Isolation and characterization of two human transcription fa... | ACCEPT | Summary: TFIIK complex membership from isolation of ERCC2/CAK. Reason: TFIIK is the CDK7-cyclin H-MAT1 kinase module of TFIIH, which also exists free as CAK. Supporting Evidence: PMID:8692841 ERCC2/CAK was isolated as a complex that exhibits CAK activity that cosediments with the three CAK components (cdk7, cyclin H, and p36/MAT1) as well as the ERCC2 (XPD) protein |
| GO:0070985 transcription factor TFIIK complex | IEA GO_REF:0000002 | ACCEPT | Summary: Electronic TFIIK complex membership. Reason: TFIIK is the CDK7-cyclin H-MAT1 kinase module of TFIIH, which also exists free as CAK. Supporting Evidence: PMID:10801852 the C terminus of MAT1 binds to the cdk7 x cyclin H complex and activates the cdk7 kinase activity |
| GO:0070985 transcription factor TFIIK complex | IMP PMID:23393140 Interaction with cyclin H/cyclin-dependent kinase 7 (CCNH/CD... | ACCEPT | Summary: TFIIK complex membership supported in the CtBP2 study by CCNH/CDK7 co-dependence. Reason: The evidence type (IMP) is unusual for complex membership, but the claim that CDK7 is in the CCNH/CDK7 (TFIIK) complex is well established and consistent with the paper. Supporting Evidence: PMID:23393140 the cyclin H/cyclin-dependent kinase 7 (CCNH/CDK7) complex interacted with CtBP2 in vivo and in vitro PMID:10801852 the C terminus of MAT1 binds to the cdk7 x cyclin H complex and activates the cdk7 kinase activity |
| GO:0106310 protein serine kinase activity | EXP PMID:20360007 Cdc25 phosphatases are required for timely assembly of CDK1-... | ACCEPT | Summary: Protein serine kinase activity (Cdk1 activation study). Reason: CDK7 phosphorylates serine residues on protein substrates, including p53 Ser33 and the Pol II CTD Ser5/Ser7. Supporting Evidence: PMID:20360007 we find that Cdc25A overexpression leads to an activation of Cdk7 and increase in Thr(161) phosphorylation of Cdk1 PMID:9372954 We have mapped a major site of phosphorylation by CAK to Ser-33 of p53 and have demonstrated as well that p53 is phosphorylated at this site in vivo |
| GO:0106310 protein serine kinase activity | EXP PMID:9372954 p53 is phosphorylated by CDK7-cyclin H in a p36MAT1-dependen... | ACCEPT | Summary: Protein serine kinase activity: CAK phosphorylates p53 at Ser33. Reason: CDK7 phosphorylates serine residues on protein substrates, including p53 Ser33 and the Pol II CTD Ser5/Ser7. Supporting Evidence: PMID:9372954 We have mapped a major site of phosphorylation by CAK to Ser-33 of p53 and have demonstrated as well that p53 is phosphorylated at this site in vivo |
| GO:0106310 protein serine kinase activity | EXP PMID:9840937 Regulation of CAK kinase activity by p53. | ACCEPT | Summary: Protein serine kinase activity in CAK assays regulated by p53. Reason: CDK7 phosphorylates serine residues on protein substrates, including p53 Ser33 and the Pol II CTD Ser5/Ser7. Supporting Evidence: PMID:9840937 The addition of wild type p53 to the kinase reactions resulted in a significant downregulation of CDK2 phosphorylation and CTD phosphorylation by the CDK activating kinase PMID:9372954 We have mapped a major site of phosphorylation by CAK to Ser-33 of p53 and have demonstrated as well that p53 is phosphorylated at this site in vivo |
| GO:0106310 protein serine kinase activity | IEA GO_REF:0000116 | ACCEPT | Summary: Rhea-based protein serine kinase activity. Reason: CDK7 phosphorylates serine residues on protein substrates, including p53 Ser33 and the Pol II CTD Ser5/Ser7. Supporting Evidence: PMID:9372954 We have mapped a major site of phosphorylation by CAK to Ser-33 of p53 and have demonstrated as well that p53 is phosphorylated at this site in vivo |
| GO:0140836 RNA polymerase II CTD heptapeptide repeat S5 kinase activity | IDA PMID:26257281 THZ1 Reveals Roles for Cdk7 in Co-transcriptional Capping an... | ACCEPT | Summary: CTD S5 kinase activity: THZ1 abolishes initiation-associated Ser5 phosphorylation. Reason: CDK7 is the TFIIH CTD kinase; chemical-genetic and THZ1 inhibition abolish initiation-associated CTD Ser5/Ser7 phosphorylation. Supporting Evidence: PMID:26257281 Inhibition of Cdk7 blocks Pol II CTD phosphorylation during initiation PMID:26257281 Cdk7 phosphorylates Ser5 and Ser7 residues, the first of several CTD phosphorylation and dephosphorylation events during the transcription cycle |
| GO:2000045 regulation of G1/S transition of mitotic cell cycle | IDA PMID:23622515 A Cdk7-Cdk4 T-loop phosphorylation cascade promotes G1 progr... | ACCEPT | Summary: Regulation of G1/S transition: CDK7 is the physiological CAK for CDK4/6 and promotes G1 progression. Reason: Analog-sensitive CDK7 inhibition rapidly abolishes CDK4/CDK6 T-loop phosphorylation, so CDK7 performs the activating step for the G1 CDKs. Supporting Evidence: PMID:23622515 Here we analyze activation of Cdk4 and Cdk6 in Cdk7as cells, and show that Cdk7 is a physiologic CAK for both cyclin D-dependent kinases PMID:23622515 this suggests that Cdk7 is a universal CAK for CDKs involved in human cell division |
| GO:0032968 positive regulation of transcription elongation by RNA polymerase II | IDA PMID:28768201 Human TFIIH Kinase CDK7 Regulates Transcription-Associated C... | NEW | Summary: CDK7 promotes the transition to productive elongation by activating CDK9 (P-TEFb) and phosphorylating DSIF/SPT5. Reason: CDK7 itself does the work: it phosphorylates the CDK9 T-loop and SPT5, and its inhibition increases promoter-proximal pausing and blocks DSIF loading. Comparator check: the CDK7 ortholog Mcs6 in S. pombe carries this process in its review, and CDK9 itself carries it. Not a substrate or necessity-only relationship. Supporting Evidence: PMID:28768201 CDK7 phosphorylates the RNA polymerase II (pol II) C-terminal domain CTD and activates the P-TEFb-associated kinase CDK9 PMID:16327805 proteins such as Pol II and transcription elongation factor Spt5, recognized efficiently only by the fully activated Cdk7 complex PMID:26257281 we conclude that THZ1 leads to reduced pausing and capping both in vitro and in cells |
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Download this section (compressed HTML)Q: GO has no specific molecular function for CDK-activating kinase activity (T-loop phosphorylation of another CDK); should a child of GO:0004672 be created so that CAK activity of CDK7, budding-yeast Cak1 and S. pombe Csk1 can be captured distinctly from the cyclin-dependent activity of the substrate CDKs?
Q: Should the GO:0008094 and GO:0000439 rows derived from purified TFIIH be redistributed to the XPB/XPD and core subunits rather than every co-purifying subunit?
Experiment: Use analog-sensitive CDK7 with phosphoproteomics in G1-synchronised cells to separate the contributions of CAK (CDK4/6/2 T-loop) and TFIIH CTD kinase activity to S-phase entry.
Type: chemical genetics / phosphoproteomics
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