CEP152

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0007099 centriole replication	IBA GO_REF:0000033	ACCEPT	Summary: PAINT node PTN000818649 is CEP152-specific, and centriole replication is independently established by human depletion and PLK4-recruitment experiments. Reason: The phylogenetic inference has coherent scope, although O94986 itself is one of its experimental seeds.
GO:0005813 centrosome	IBA GO_REF:0000033	ACCEPT	Summary: The CEP152-specific PAINT node and extensive human microscopy/biochemistry both place active CEP152 at the centrosome. Reason: The propagated location agrees with multiple independent direct human studies.
GO:0005813 centrosome	IEA GO_REF:0000044	ACCEPT	Summary: UniProt's centrosome location is supported by numerous direct human localization and loss-of-function studies.
GO:0005814 centriole	IEA GO_REF:0000044	ACCEPT	Summary: UniProt's centriole location agrees with super-resolution placement of CEP152 around the proximal parent/daughter centriole region.
GO:0005515 protein binding	IPI PMID:21059844 Cep152 acts as a scaffold for recruitment of Plk4 and CPAP t...	MODIFY	Summary: Direct CEP152-PLK4 interaction is well supported, but generic protein binding obscures the informative kinase-binding relationship. Reason: Replace the uninformative generic interaction term with protein kinase binding. Proposed replacements: protein kinase binding
GO:0005515 protein binding	IPI PMID:24997597 Molecular basis for unidirectional scaffold switching of hum...	MODIFY	Summary: Structural and cellular experiments establish PLK4 binding by CEP152, for which protein kinase binding is the more informative term. Reason: Replace generic protein binding with the specific supported kinase-binding activity. Proposed replacements: protein kinase binding
GO:0005515 protein binding	IPI PMID:26188084 STIL binding to Polo-box 3 of PLK4 regulates centriole dupli...	MODIFY	Summary: The reported interactor is the protein kinase PLK4, so the broad binding term can be made functionally specific. Reason: Use protein kinase binding for the experimentally detected PLK4 interaction. Proposed replacements: protein kinase binding
GO:0005515 protein binding	IPI PMID:26297806 Centriolar satellites assemble centrosomal microcephaly prot...	MARK AS OVER ANNOTATED	Summary: CEP131 association is plausible within the centrosomal microcephaly-protein network, but generic protein binding does not describe a biological activity. Reason: Retain the interaction as evidence in the literature record, not as an informative GO molecular function.
GO:0005515 protein binding	IPI PMID:26496610 A human interactome in three quantitative dimensions organiz...	MODIFY	Summary: The quantitative interactome detects CEP152 with PLK4; kinase binding is more informative than generic protein binding. Reason: Replace the generic interaction term with protein kinase binding. Proposed replacements: protein kinase binding
GO:0005515 protein binding	IPI PMID:26638075 A Dynamic Protein Interaction Landscape of the Human Centros...	MODIFY	Summary: The centrosome-cilium interaction map supports CEP152 association with PLK4, a relationship captured more precisely as kinase binding. Reason: Replace generic protein binding with the specific PLK4-binding molecular function. Proposed replacements: protein kinase binding
GO:0005515 protein binding	IPI PMID:35709258 Spatial centrosome proteome of human neural cells uncovers d...	MARK AS OVER ANNOTATED	Summary: MOV10 was recovered in a neural-cell centrosome proximity/interactome study, but the generic binding term supplies no mechanistic function for CEP152. Reason: The high-throughput association is contextual evidence and does not warrant an uninformative binding-function annotation.
GO:0007099 centriole replication	IEA GO_REF:0000107	ACCEPT	Summary: Mouse-to-human orthology transfer is appropriate because direct human depletion studies independently show a requirement for centriole replication.
GO:0098535 de novo centriole assembly involved in multi-ciliated epithelial cell differentiation	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: Mouse multiciliated-cell studies support a conserved CEP152 role in deuterosome-associated centriole amplification, but direct human evidence is limited. Reason: Retain as a credible specialized epithelial context rather than the universal core function.
GO:0098536 deuterosome	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: Mouse Cep152 localizes to DEUP1-positive deuterosomes during multiciliated-cell differentiation; the human call is an orthology transfer. Reason: Retain as a specialized multiciliated-cell location, distinct from the core parent-centriole/PCM site.
GO:0030674 protein-macromolecule adaptor activity	IDA PMID:20852615 Asterless is a scaffold for the onset of centriole assembly.	ACCEPT	Summary: CEP152 uses separated regions to engage PLK4 and CPAP/CENPJ, directly supporting a non-catalytic adaptor/scaffold activity. Supporting Evidence: file:human/CEP152/CEP152-deep-research-manual.md CEP152 is a non-enzymatic, coiled-coil scaffold of the pericentriolar material and proximal parent-centriole region.
GO:0005813 centrosome	IPI PMID:30858376 Molecular architecture of a cylindrical self-assembly at hum...	ACCEPT	Summary: Biochemical assembly with the centrosomal scaffold CEP63 and cell imaging support centrosomal localization of the complex.
GO:0007099 centriole replication	NAS PMID:30858376 Molecular architecture of a cylindrical self-assembly at hum...	ACCEPT	Summary: The cited study shows that disrupting CEP63-CEP152 self-assembly abrogates PLK4-mediated centriole duplication.
GO:0051298 centrosome duplication	NAS PMID:30858376 Molecular architecture of a cylindrical self-assembly at hum...	ACCEPT	Summary: Higher-order CEP63-CEP152 assembly is required for the PLK4-dependent centriole pathway that underlies centrosome duplication.
GO:0005813 centrosome	IDA GO_REF:0000052	ACCEPT	Summary: Human Protein Atlas immunofluorescence supports centrosome localization, consistent with gene-specific microscopy studies.
GO:0036064 ciliary basal body	IDA GO_REF:0000052	KEEP AS NON CORE	Summary: Immunofluorescence supports basal-body localization, consistent with the centriole-derived nature of basal bodies. Reason: Basal-body localization is a differentiated ciliary context rather than CEP152's defining centriole-duplication site.
GO:0007099 centriole replication	IDA PMID:21059844 Cep152 acts as a scaffold for recruitment of Plk4 and CPAP t...	ACCEPT	Summary: CEP152 depletion prevents centrosomal PLK4 recruitment and normal or PLK4-induced centriole duplication.
GO:0120098 procentriole	IDA PMID:24997597 Molecular basis for unidirectional scaffold switching of hum...	ACCEPT	Summary: Super-resolution imaging places CEP152 in the outer ring assembled around the daughter centriole/procentriole site.
GO:0120099 procentriole replication complex	IPI PMID:24997597 Molecular basis for unidirectional scaffold switching of hum...	ACCEPT	Summary: CEP152 is a structural member of the PLK4-recruiting procentriole replication scaffold demonstrated by interaction and localization experiments.
GO:0005829 cytosol	TAS Reactome:R-HSA-2574840	KEEP AS NON CORE	Summary: Reactome places CEP152 in a soluble cytosolic pool for this event; the location is plausible but broad and is not an independent localization experiment.
GO:0005829 cytosol	TAS Reactome:R-HSA-2574845	KEEP AS NON CORE	Summary: Reactome places CEP152 in a soluble cytosolic pool for this event; the location is plausible but broad and is not an independent localization experiment.
GO:0005829 cytosol	TAS Reactome:R-HSA-3000310	KEEP AS NON CORE	Summary: Reactome places CEP152 in a soluble cytosolic pool for this event; the location is plausible but broad and is not an independent localization experiment.
GO:0005829 cytosol	TAS Reactome:R-HSA-3000319	KEEP AS NON CORE	Summary: Reactome places CEP152 in a soluble cytosolic pool for this event; the location is plausible but broad and is not an independent localization experiment.
GO:0005829 cytosol	TAS Reactome:R-HSA-380272	KEEP AS NON CORE	Summary: Reactome places CEP152 in a soluble cytosolic pool for this event; the location is plausible but broad and is not an independent localization experiment.
GO:0005829 cytosol	TAS Reactome:R-HSA-380283	KEEP AS NON CORE	Summary: Reactome places CEP152 in a soluble cytosolic pool for this event; the location is plausible but broad and is not an independent localization experiment.
GO:0005829 cytosol	TAS Reactome:R-HSA-380294	KEEP AS NON CORE	Summary: Reactome places CEP152 in a soluble cytosolic pool for this event; the location is plausible but broad and is not an independent localization experiment.
GO:0005829 cytosol	TAS Reactome:R-HSA-380303	KEEP AS NON CORE	Summary: Reactome places CEP152 in a soluble cytosolic pool for this event; the location is plausible but broad and is not an independent localization experiment.
GO:0005829 cytosol	TAS Reactome:R-HSA-380311	KEEP AS NON CORE	Summary: Reactome places CEP152 in a soluble cytosolic pool for this event; the location is plausible but broad and is not an independent localization experiment.
GO:0005829 cytosol	TAS Reactome:R-HSA-380316	KEEP AS NON CORE	Summary: Reactome places CEP152 in a soluble cytosolic pool for this event; the location is plausible but broad and is not an independent localization experiment.
GO:0005829 cytosol	TAS Reactome:R-HSA-380455	KEEP AS NON CORE	Summary: Reactome places CEP152 in a soluble cytosolic pool for this event; the location is plausible but broad and is not an independent localization experiment.
GO:0005829 cytosol	TAS Reactome:R-HSA-380508	KEEP AS NON CORE	Summary: Reactome places CEP152 in a soluble cytosolic pool for this event; the location is plausible but broad and is not an independent localization experiment.
GO:0005829 cytosol	TAS Reactome:R-HSA-5617816	KEEP AS NON CORE	Summary: Reactome places CEP152 in a soluble cytosolic pool for this event; the location is plausible but broad and is not an independent localization experiment.
GO:0005829 cytosol	TAS Reactome:R-HSA-5626220	KEEP AS NON CORE	Summary: Reactome places CEP152 in a soluble cytosolic pool for this event; the location is plausible but broad and is not an independent localization experiment.
GO:0005829 cytosol	TAS Reactome:R-HSA-5626223	KEEP AS NON CORE	Summary: Reactome places CEP152 in a soluble cytosolic pool for this event; the location is plausible but broad and is not an independent localization experiment.
GO:0005829 cytosol	TAS Reactome:R-HSA-5626227	KEEP AS NON CORE	Summary: Reactome places CEP152 in a soluble cytosolic pool for this event; the location is plausible but broad and is not an independent localization experiment.
GO:0005829 cytosol	TAS Reactome:R-HSA-5626228	KEEP AS NON CORE	Summary: Reactome places CEP152 in a soluble cytosolic pool for this event; the location is plausible but broad and is not an independent localization experiment.
GO:0005829 cytosol	TAS Reactome:R-HSA-5626681	KEEP AS NON CORE	Summary: Reactome places CEP152 in a soluble cytosolic pool for this event; the location is plausible but broad and is not an independent localization experiment.
GO:0005829 cytosol	TAS Reactome:R-HSA-5626699	KEEP AS NON CORE	Summary: Reactome places CEP152 in a soluble cytosolic pool for this event; the location is plausible but broad and is not an independent localization experiment.
GO:0005829 cytosol	TAS Reactome:R-HSA-5638009	KEEP AS NON CORE	Summary: Reactome places CEP152 in a soluble cytosolic pool for this event; the location is plausible but broad and is not an independent localization experiment.
GO:0005829 cytosol	TAS Reactome:R-HSA-8853405	KEEP AS NON CORE	Summary: Reactome places CEP152 in a soluble cytosolic pool for this event; the location is plausible but broad and is not an independent localization experiment.
GO:0005829 cytosol	TAS Reactome:R-HSA-8853419	KEEP AS NON CORE	Summary: Reactome places CEP152 in a soluble cytosolic pool for this event; the location is plausible but broad and is not an independent localization experiment.
GO:0005515 protein binding	IPI PMID:35849559 The ciliopathy protein CCDC66 controls mitotic progression a...	MARK AS OVER ANNOTATED	Summary: Overexpression co-immunoprecipitation supports association with CCDC66, but generic protein binding is not an informative CEP152 activity. Reason: Preserve the interaction as supporting context rather than a generic GO molecular function.
GO:0005813 centrosome	IDA PMID:35849559 The ciliopathy protein CCDC66 controls mitotic progression a...	ACCEPT	Summary: The CCDC66 study directly visualizes CEP152 at centrosomes, consistent with extensive independent localization evidence.
GO:0005814 centriole	IDA PMID:32060285 CEP44 ensures the formation of bona fide centriole wall, a r...	ACCEPT	Summary: The CEP44 study uses CEP152 as the PLK4-recruiting centriolar adaptor and directly supports centriolar localization.
GO:0005814 centriole	IDA PMID:22020124 The human microcephaly protein STIL interacts with CPAP and ...	ACCEPT	Summary: The cached abstract foregrounds STIL/CPAP rather than the CEP152 assay, but the curator's full-text annotation is strongly corroborated by independent CEP152 localization studies. Reason: Do not overrule an experimental curator annotation from an abstract-only cache when the biological conclusion is independently secure.
GO:0000242 pericentriolar material	IDA PMID:26337392 MDM1 is a microtubule-binding protein that negatively regula...	ACCEPT	Summary: Microscopy resolves CEP152 as a ring in the pericentriolar material around centrioles, matching its structural scaffold role.
GO:0005814 centriole	IDA PMID:26337392 MDM1 is a microtubule-binding protein that negatively regula...	ACCEPT	Summary: Direct imaging in the cited study supports CEP152 localization at the centriole/pericentriolar interface.
GO:0007099 centriole replication	ISS GO_REF:0000024	ACCEPT	Summary: Curator sequence-similarity transfer is consistent with conserved vertebrate CEP152 architecture and direct human duplication phenotypes.
GO:0098535 de novo centriole assembly involved in multi-ciliated epithelial cell differentiation	ISS GO_REF:0000024	KEEP AS NON CORE	Summary: Sequence-similarity transfer from vertebrate CEP152 is consistent with direct mouse multiciliated-cell evidence. Reason: Retain as a specialized multiciliated epithelial role, not the ubiquitous core process.
GO:0098536 deuterosome	ISS GO_REF:0000024	KEEP AS NON CORE	Summary: The inferred deuterosome localization agrees with direct mouse Cep152 localization during centriole amplification. Reason: Retain as a specialized multiciliated-cell location.
GO:0005813 centrosome	IDA PMID:21399614 Novel asymmetrically localizing components of human centroso...	ACCEPT	Summary: Complementary centrosome proteomics and localization experiments identify CEP152 as a human centrosomal component.
GO:0005515 protein binding	IPI PMID:20852615 Asterless is a scaffold for the onset of centriole assembly.	MODIFY	Summary: CEP152 binds CENPJ/CPAP through a region distinct from its PLK4-binding region, establishing a two-ended molecular scaffold. Reason: The mechanistic result is better represented by the existing protein-macromolecule adaptor activity annotation. Proposed replacements: protein-macromolecule adaptor activity
GO:0005515 protein binding	IPI PMID:21131973 CEP152 is a genome maintenance protein disrupted in Seckel s...	MARK AS OVER ANNOTATED	Summary: The CINP interaction may contribute to the reported genome-maintenance phenotype, but generic protein binding is not an informative molecular function. Reason: The accessible abstract does not establish a more specific CEP152 activity for this contact; do not overinterpret it.
GO:0005813 centrosome	IDA PMID:21131973 CEP152 is a genome maintenance protein disrupted in Seckel s...	ACCEPT	Summary: The Seckel-syndrome study identifies CEP152 as a centrosomal protein, consistent with direct localization and disease-allele mislocalization evidence.
GO:0019901 protein kinase binding	IPI PMID:20852615 Asterless is a scaffold for the onset of centriole assembly.	ACCEPT	Summary: The CEP152 N-terminal region binds the cryptic Polo-box of the protein kinase PLK4, directly supporting kinase binding.
GO:0019901 protein kinase binding	IPI PMID:21059844 Cep152 acts as a scaffold for recruitment of Plk4 and CPAP t...	ACCEPT	Summary: Direct interaction mapping establishes binding between CEP152's N terminus and the PLK4 cryptic Polo-box.
GO:0051298 centrosome duplication	IMP PMID:20852615 Asterless is a scaffold for the onset of centriole assembly.	ACCEPT	Summary: CEP152 depletion causes centrosome-duplication failure and overexpression drives amplification, directly supporting the process annotation.
GO:0090307 mitotic spindle assembly	IMP PMID:34878135 The APC/C targets the Cep152-Cep63 complex at the centrosome...	NEW	Summary: APC/C-dependent turnover of centrosomal CEP152 releases CEP57 for pericentrin recruitment; stabilizing an APC/C-resistant CEP152 mutant reduces microtubule nucleation and increases chromosome mis-segregation. Reason: This direct human-cell perturbation study establishes a CEP152-dependent mitotic spindle-assembly mechanism that is absent from the current GOA. Supporting Evidence: PMID:34878135 This negative feedback loop controlling APC/C localization is required for proper assembly of the mitotic spindle.

Gene Description

Existing Annotations Review

Core Functions

During mitosis, centrosomal CEP152 organizes a temporally regulated complex with CEP63 and CEP57 and recruits APC/C. APC/C-dependent CEP152 ubiquitylation releases CEP57 so that it can bind pericentrin, promoting microtubule nucleation and proper mitotic spindle assembly.

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