CHAT

direct transfer of the acetyl group

Authored source-scope assessment: Exact positive records intact:EBI-25837694, intact:EBI-25994476, intact:EBI-26148623 match human CHAT P28329-3 and P01106 in the array, pooling and validated yeast two-hybrid stages of PMID:32814053. No partner fragment boundary is supplied in these exact records. These are related stages of one study, not three independent physiological replications. Official partner identity and functional annotation establish the DNA-binding transcription-factor class, permitting this specific binding refinement. Neither DNA binding by CHAT nor transcriptional coactivation, repression or regulation of that partner is established by the screen. The original Methods systematically excluded autoactivating bait strains, selected HIS3/URA3 growth and lacZ reporter positives, and retested pooled hits pairwise. These controls support the reported positive association but do not eliminate all assay artifacts. The three recorded stages are related tests within one study, not independent physiological replications. No CHAT-specific mammalian confirmation, endogenous coexpression or native compartmental encounter is established. This non-core refinement identifies the bound partner class; it does not increase the evidence strength or assign that partner's activity to CHAT.

Authored source-scope assessment: Exact positive records intact:EBI-25837637, intact:EBI-25994419, intact:EBI-26148566 match human CHAT P28329-3 and P10809 in the array, pooling and validated yeast two-hybrid stages of PMID:32814053. No partner fragment boundary is supplied in these exact records. These are related stages of one study, not three independent physiological replications. The verified HSPD1 chaperonin identity supports the narrower partner-class term. The assay does not make CHAT a chaperone or cochaperone, establish folding assistance, or demonstrate a native mitochondrial encounter. The original Methods systematically excluded autoactivating bait strains, selected HIS3/URA3 growth and lacZ reporter positives, and retested pooled hits pairwise. These controls support the reported positive association but do not eliminate all assay artifacts. The three recorded stages are related tests within one study, not independent physiological replications. No CHAT-specific mammalian confirmation, endogenous coexpression or native compartmental encounter is established. This non-core refinement identifies the bound partner class; it does not increase the evidence strength or assign that partner's activity to CHAT.

Authored source-scope assessment: Exact positive records intact:EBI-25837808, intact:EBI-25994590, intact:EBI-26148737 match human CHAT P28329-3 and P15407 in the array, pooling and validated yeast two-hybrid stages of PMID:32814053. No partner fragment boundary is supplied in these exact records. These are related stages of one study, not three independent physiological replications. Official partner identity and functional annotation establish the DNA-binding transcription-factor class, permitting this specific binding refinement. Neither DNA binding by CHAT nor transcriptional coactivation, repression or regulation of that partner is established by the screen. The original Methods systematically excluded autoactivating bait strains, selected HIS3/URA3 growth and lacZ reporter positives, and retested pooled hits pairwise. These controls support the reported positive association but do not eliminate all assay artifacts. The three recorded stages are related tests within one study, not independent physiological replications. No CHAT-specific mammalian confirmation, endogenous coexpression or native compartmental encounter is established. This non-core refinement identifies the bound partner class; it does not increase the evidence strength or assign that partner's activity to CHAT.

Authored source-scope assessment: Exact positive records intact:EBI-25837653, intact:EBI-25994435, intact:EBI-26148582 match human CHAT P28329-3 and P17275 in the array, pooling and validated yeast two-hybrid stages of PMID:32814053. No partner fragment boundary is supplied in these exact records. These are related stages of one study, not three independent physiological replications. Official partner identity and functional annotation establish the DNA-binding transcription-factor class, permitting this specific binding refinement. Neither DNA binding by CHAT nor transcriptional coactivation, repression or regulation of that partner is established by the screen. The original Methods systematically excluded autoactivating bait strains, selected HIS3/URA3 growth and lacZ reporter positives, and retested pooled hits pairwise. These controls support the reported positive association but do not eliminate all assay artifacts. The three recorded stages are related tests within one study, not independent physiological replications. No CHAT-specific mammalian confirmation, endogenous coexpression or native compartmental encounter is established. This non-core refinement identifies the bound partner class; it does not increase the evidence strength or assign that partner's activity to CHAT.

Authored source-scope assessment: Exact positive records intact:EBI-25837613, intact:EBI-25994395, intact:EBI-26148542 match human CHAT P28329-3 and P23771 in the array, pooling and validated yeast two-hybrid stages of PMID:32814053. No partner fragment boundary is supplied in these exact records. These are related stages of one study, not three independent physiological replications. Official partner identity and functional annotation establish the DNA-binding transcription-factor class, permitting this specific binding refinement. Neither DNA binding by CHAT nor transcriptional coactivation, repression or regulation of that partner is established by the screen. The original Methods systematically excluded autoactivating bait strains, selected HIS3/URA3 growth and lacZ reporter positives, and retested pooled hits pairwise. These controls support the reported positive association but do not eliminate all assay artifacts. The three recorded stages are related tests within one study, not independent physiological replications. No CHAT-specific mammalian confirmation, endogenous coexpression or native compartmental encounter is established. This non-core refinement identifies the bound partner class; it does not increase the evidence strength or assign that partner's activity to CHAT.

Authored source-scope assessment: Exact positive records intact:EBI-25837621, intact:EBI-25994403, intact:EBI-26148550 match human CHAT P28329-3 and P55318 in the array, pooling and validated yeast two-hybrid stages of PMID:32814053. No partner fragment boundary is supplied in these exact records. These are related stages of one study, not three independent physiological replications. Official partner identity and functional annotation establish the DNA-binding transcription-factor class, permitting this specific binding refinement. Neither DNA binding by CHAT nor transcriptional coactivation, repression or regulation of that partner is established by the screen. The original Methods systematically excluded autoactivating bait strains, selected HIS3/URA3 growth and lacZ reporter positives, and retested pooled hits pairwise. These controls support the reported positive association but do not eliminate all assay artifacts. The three recorded stages are related tests within one study, not independent physiological replications. No CHAT-specific mammalian confirmation, endogenous coexpression or native compartmental encounter is established. This non-core refinement identifies the bound partner class; it does not increase the evidence strength or assign that partner's activity to CHAT.

Authored source-scope assessment: Exact positive records intact:EBI-25837736, intact:EBI-25994518, intact:EBI-26148665 match human CHAT P28329-3 and Q04206 in the array, pooling and validated yeast two-hybrid stages of PMID:32814053. No partner fragment boundary is supplied in these exact records. These are related stages of one study, not three independent physiological replications. Official partner identity and functional annotation establish the DNA-binding transcription-factor class, permitting this specific binding refinement. Neither DNA binding by CHAT nor transcriptional coactivation, repression or regulation of that partner is established by the screen. The original Methods systematically excluded autoactivating bait strains, selected HIS3/URA3 growth and lacZ reporter positives, and retested pooled hits pairwise. These controls support the reported positive association but do not eliminate all assay artifacts. The three recorded stages are related tests within one study, not independent physiological replications. No CHAT-specific mammalian confirmation, endogenous coexpression or native compartmental encounter is established. This non-core refinement identifies the bound partner class; it does not increase the evidence strength or assign that partner's activity to CHAT.

Authored source-scope assessment: Exact positive records intact:EBI-25838445, intact:EBI-25995199, intact:EBI-26149346 match human CHAT P28329-3 and Q3KNS6-3 in the array, pooling and validated yeast two-hybrid stages of PMID:32814053. The partner record specifies sufficient binding region:82-513. The official Q3KNS6 record assigns DNA-binding transcription-factor and sequence-specific regulatory-region DNA-binding activities by IBA. Reconstruction using VSP_057606 shows that isoform-3 residues 82–513 are exactly the entire 432-residue displayed sequence, including the KRAB domain and all ten annotated zinc fingers. Thus the recorded sufficient-binding region preserves the curated partner class. This is inferred class evidence, not experimental validation of every transcriptional property of the partner. The original Methods systematically excluded autoactivating bait strains, selected HIS3/URA3 growth and lacZ reporter positives, and retested pooled hits pairwise. These controls support the reported positive association but do not eliminate all assay artifacts. The three recorded stages are related tests within one study, not independent physiological replications. No CHAT-specific mammalian confirmation, endogenous coexpression or native compartmental encounter is established. This non-core refinement identifies the bound partner class; it does not increase the evidence strength or assign that partner's activity to CHAT.

Authored source-scope assessment: Exact positive records intact:EBI-25838298, intact:EBI-25995052, intact:EBI-26149199 match human CHAT P28329-3 and Q6XD76 in the array, pooling and validated yeast two-hybrid stages of PMID:32814053. No partner fragment boundary is supplied in these exact records. The official Q6XD76 record positively assigns RNA polymerase II DNA-binding transcription-factor activity by ISA and sequence-specific DNA binding by IBA, with a bHLH domain at residues 72–124. Its tentative biological-function prose does not invalidate these positive curated class inferences. This refinement uses the inferred partner class, without claiming that the CHAT screen measured DNA recognition or transcriptional regulation. The original Methods systematically excluded autoactivating bait strains, selected HIS3/URA3 growth and lacZ reporter positives, and retested pooled hits pairwise. These controls support the reported positive association but do not eliminate all assay artifacts. The three recorded stages are related tests within one study, not independent physiological replications. No CHAT-specific mammalian confirmation, endogenous coexpression or native compartmental encounter is established. This non-core refinement identifies the bound partner class; it does not increase the evidence strength or assign that partner's activity to CHAT.

82-kDa form was only present in nucleus

Authored source-scope assessment: R-HSA-1483191 genuinely includes R-HSA-264622 among PC-synthesis, lipid-transport and choline-recycling events. Its exact CHAT catalyst264613 consumes choline and acetyl-CoA and produces acetylcholine and CoA in the cytosol. GO:0006656 denotes formation of phosphatidylcholine, whereas this inspected step makes acetylcholine. The pathway membership is real, but it does not establish CHAT performing PC-forming chemistry, a structural/cofactor step in that synthesis, or regulation of that process. Remove this source-scoped involved_in GO projection, while preserving the genuine pathway membership, while retaining the independently supported acetylcholine-biosynthesis assertion already present. This does not assert that CHAT can never influence choline or lipid metabolism.

Authored source-scope assessment: The original source explicitly asserts involved_in GO:0006836. Its definition concerns directed neurotransmitter movement, whereas official R-HSA-264642 includes CHAT reaction R-HSA-264622 as the synthesis step alongside distinct uptake, vesicle loading, acidification, docking and release events. Catalyst 264613 consumes choline and acetyl-CoA and forms acetylcholine plus CoA in the cytosol. No CHAT-performed transport, transport scaffold or transport-cofactor step is demonstrated by this inspected source. Remove this source-scoped process projection while retaining the genuine pathway membership and existing acetylcholine synthesis and synaptic-transmission assertions. This does not claim that CHAT can never regulate transport, nor does the separate SLC5A7 expression phenotype make the inspected synthesis reaction a transport step.

defect in ACh resynthesis or vesicular filling