CHMP4A encodes a Snf7-family core subunit of ESCRT-III. Its best-supported function is non-enzymatic ESCRT-III polymerization on endosomal/MVB and related membranes, where CHMP4A-containing filaments bend membranes and support reverse-topology budding/fission for intraluminal vesicle formation, MVB cargo sorting, and downstream endolysosomal degradation. CHMP4A also participates in autophagy/autophagosome contexts, plasma membrane repair, cytokinetic abscission, nuclear envelope repair, and viral budding as topologically related ESCRT output contexts, but those should not obscure the core ESCRT-III membrane-remodeling role.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005635 nuclear envelope | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: nuclear envelope. Reason: Nuclear envelope or nuclear pore repair/reassembly is a supported ESCRT-III context but is secondary to CHMP4A core ESCRT-III membrane-remodeling function. Supporting Evidence: PMID:26040713 ESCRT-III controls nuclear envelope reformation UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0031468 nuclear membrane reassembly | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: nuclear membrane reassembly. Reason: Nuclear envelope or nuclear pore repair/reassembly is a supported ESCRT-III context but is secondary to CHMP4A core ESCRT-III membrane-remodeling function. Supporting Evidence: PMID:26040713 ESCRT-III controls nuclear envelope reformation UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0000815 ESCRT III complex | IBA GO_REF:0000033 | ACCEPT | Summary: Supported core CHMP4A ESCRT-III/MVB membrane-remodeling annotation: ESCRT III complex. Reason: CHMP4A is a core ESCRT-III/Snf7-family subunit that polymerizes on endosomal and MVB membranes to promote membrane budding/fission and MVB cargo sorting. Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:18209100 Multivesicular bodies (MVBs) are mid-stage endosomes that contain intraluminal vesicles UniProt:Q9BY43 Cytoplasmic vesicle membrane. Late endosome membrane UniProt:Q9BY43 Membrane-associated |
| GO:0009898 cytoplasmic side of plasma membrane | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: cytoplasmic side of plasma membrane. Reason: This location or output context is supported or plausible for CHMP4A but is broad or secondary relative to its core ESCRT-III MVB/endosomal membrane-remodeling role. Supporting Evidence: UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks UniProt:Q9BY43 Cytoplasmic vesicle membrane. Late endosome membrane UniProt:Q9BY43 Membrane-associated |
| GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway | IBA GO_REF:0000033 | ACCEPT | Summary: Supported core CHMP4A ESCRT-III/MVB membrane-remodeling annotation: late endosome to vacuole transport via multivesicular body sorting pathway. Reason: CHMP4A is a core ESCRT-III/Snf7-family subunit that polymerizes on endosomal and MVB membranes to promote membrane budding/fission and MVB cargo sorting. Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:18209100 Multivesicular bodies (MVBs) are mid-stage endosomes that contain intraluminal vesicles UniProt:Q9BY43 Cytoplasmic vesicle membrane. Late endosome membrane UniProt:Q9BY43 Membrane-associated |
| GO:0000776 kinetochore | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: kinetochore. Reason: Cytokinesis/midbody and spindle annotations are supported ESCRT output contexts, but they are not the central endolysosomal/proteostasis function emphasized for CHMP4A. Supporting Evidence: PMID:22724069 Borealin interacts directly with the Snf7 components of ESCRT-III PMID:22724069 controls abscission timing through inhibition of ESCRT-III Snf7 polymerization UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0001778 plasma membrane repair | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: plasma membrane repair. Reason: Plasma membrane repair is a valid ESCRT output context, but the core CHMP4A function remains ESCRT-III membrane remodeling in endosomal/MVB and related compartments. Supporting Evidence: PMID:24482116 ESCRT proteins were recruited within seconds to plasma membrane wounds PMID:24482116 repair of certain wounds is ensured by ESCRT-mediated extracellular shedding of wounded portions UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0005643 nuclear pore | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: nuclear pore. Reason: Nuclear envelope or nuclear pore repair/reassembly is a supported ESCRT-III context but is secondary to CHMP4A core ESCRT-III membrane-remodeling function. Supporting Evidence: PMID:26040713 ESCRT-III controls nuclear envelope reformation UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0005765 lysosomal membrane | IEA GO_REF:0000117 | ACCEPT | Summary: Supported PN-relevant autophagy/endolysosomal CHMP4A context: lysosomal membrane. Reason: CHMP4A/Snf7-1 depletion causes autophagosome accumulation in human neurons, and ESCRT/MVB function is required for autophagic degradation; this supports retaining autophagy and autophagosome-context annotations without claiming CHMP4A alone was directly assayed as the phagophore-sealing effector. Supporting Evidence: PMID:21975012 SiRNA knockdown of hSnf7-1 in human neurons leads to autophagosome accumulation PMID:17984323 Functional multivesicular bodies are required for autophagic clearance UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0005828 kinetochore microtubule | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: kinetochore microtubule. Reason: Cytokinesis/midbody and spindle annotations are supported ESCRT output contexts, but they are not the central endolysosomal/proteostasis function emphasized for CHMP4A. Supporting Evidence: PMID:22724069 Borealin interacts directly with the Snf7 components of ESCRT-III PMID:22724069 controls abscission timing through inhibition of ESCRT-III Snf7 polymerization UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0007034 vacuolar transport | IEA GO_REF:0000002 | MODIFY | Summary: The broad vacuolar transport label is less precise than CHMP4A MVB/endosomal sorting function. Reason: CHMP4A is best represented by ESCRT-III-mediated MVB sorting and ILV/membrane fission, not generic vacuolar transport. Proposed replacements: multivesicular body sorting pathway Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks |
| GO:0007080 mitotic metaphase chromosome alignment | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: mitotic metaphase chromosome alignment. Reason: Cytokinesis/midbody and spindle annotations are supported ESCRT output contexts, but they are not the central endolysosomal/proteostasis function emphasized for CHMP4A. Supporting Evidence: PMID:22724069 Borealin interacts directly with the Snf7 components of ESCRT-III PMID:22724069 controls abscission timing through inhibition of ESCRT-III Snf7 polymerization UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0030496 midbody | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: midbody. Reason: Cytokinesis/midbody and spindle annotations are supported ESCRT output contexts, but they are not the central endolysosomal/proteostasis function emphasized for CHMP4A. Supporting Evidence: PMID:22724069 Borealin interacts directly with the Snf7 components of ESCRT-III PMID:22724069 controls abscission timing through inhibition of ESCRT-III Snf7 polymerization UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0030659 cytoplasmic vesicle membrane | IEA GO_REF:0000044 | ACCEPT | Summary: Supported core CHMP4A ESCRT-III/MVB membrane-remodeling annotation: cytoplasmic vesicle membrane. Reason: CHMP4A is a core ESCRT-III/Snf7-family subunit that polymerizes on endosomal and MVB membranes to promote membrane budding/fission and MVB cargo sorting. Supporting Evidence: UniProt:Q9BY43 Cytoplasmic vesicle membrane. Late endosome membrane UniProt:Q9BY43 Membrane-associated PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:18209100 Multivesicular bodies (MVBs) are mid-stage endosomes that contain intraluminal vesicles |
| GO:0031468 nuclear membrane reassembly | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: nuclear membrane reassembly. Reason: Nuclear envelope or nuclear pore repair/reassembly is a supported ESCRT-III context but is secondary to CHMP4A core ESCRT-III membrane-remodeling function. Supporting Evidence: PMID:26040713 ESCRT-III controls nuclear envelope reformation UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0031902 late endosome membrane | IEA GO_REF:0000044 | ACCEPT | Summary: Supported core CHMP4A ESCRT-III/MVB membrane-remodeling annotation: late endosome membrane. Reason: CHMP4A is a core ESCRT-III/Snf7-family subunit that polymerizes on endosomal and MVB membranes to promote membrane budding/fission and MVB cargo sorting. Supporting Evidence: UniProt:Q9BY43 Cytoplasmic vesicle membrane. Late endosome membrane UniProt:Q9BY43 Membrane-associated PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:18209100 Multivesicular bodies (MVBs) are mid-stage endosomes that contain intraluminal vesicles |
| GO:0032585 multivesicular body membrane | IEA GO_REF:0000117 | ACCEPT | Summary: Supported core CHMP4A ESCRT-III/MVB membrane-remodeling annotation: multivesicular body membrane. Reason: CHMP4A is a core ESCRT-III/Snf7-family subunit that polymerizes on endosomal and MVB membranes to promote membrane budding/fission and MVB cargo sorting. Supporting Evidence: UniProt:Q9BY43 Cytoplasmic vesicle membrane. Late endosome membrane UniProt:Q9BY43 Membrane-associated PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:18209100 Multivesicular bodies (MVBs) are mid-stage endosomes that contain intraluminal vesicles |
| GO:0036258 multivesicular body assembly | IEA GO_REF:0000117 | ACCEPT | Summary: Supported core CHMP4A ESCRT-III/MVB membrane-remodeling annotation: multivesicular body assembly. Reason: CHMP4A is a core ESCRT-III/Snf7-family subunit that polymerizes on endosomal and MVB membranes to promote membrane budding/fission and MVB cargo sorting. Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:18209100 Multivesicular bodies (MVBs) are mid-stage endosomes that contain intraluminal vesicles UniProt:Q9BY43 Cytoplasmic vesicle membrane. Late endosome membrane UniProt:Q9BY43 Membrane-associated |
| GO:0039702 viral budding via host ESCRT complex | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: viral budding via host ESCRT complex. Reason: Viral budding is directly supported as ESCRT hijacking but is not an endogenous core CHMP4A proteostasis function. Supporting Evidence: PMID:18511562 ALIX protein must bind and recruit CHMP4 subunits of the ESCRT-III complex PMID:24878737 Structure of cellular ESCRT-III spirals and their relationship to HIV budding UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0042803 protein homodimerization activity | IEA GO_REF:0000117 | MODIFY | Summary: protein homodimerization activity captures CHMP4A self-association but is less accurate than ESCRT-III polymerization and membrane bending. Reason: CHMP4A forms curved ESCRT-III filaments/polymers that bend membranes; homodimer or identical-protein binding terms are weaker molecular descriptions. Proposed replacements: protein polymerization membrane bending activity Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks |
| GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway | IEA GO_REF:0000117 | ACCEPT | Summary: Supported core CHMP4A ESCRT-III/MVB membrane-remodeling annotation: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway. Reason: CHMP4A is a core ESCRT-III/Snf7-family subunit that polymerizes on endosomal and MVB membranes to promote membrane budding/fission and MVB cargo sorting. Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:18209100 Multivesicular bodies (MVBs) are mid-stage endosomes that contain intraluminal vesicles UniProt:Q9BY43 Cytoplasmic vesicle membrane. Late endosome membrane UniProt:Q9BY43 Membrane-associated |
| GO:0046761 viral budding from plasma membrane | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: viral budding from plasma membrane. Reason: Viral budding is directly supported as ESCRT hijacking but is not an endogenous core CHMP4A proteostasis function. Supporting Evidence: PMID:18511562 ALIX protein must bind and recruit CHMP4 subunits of the ESCRT-III complex PMID:24878737 Structure of cellular ESCRT-III spirals and their relationship to HIV budding UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0061952 midbody abscission | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: midbody abscission. Reason: Cytokinesis/midbody and spindle annotations are supported ESCRT output contexts, but they are not the central endolysosomal/proteostasis function emphasized for CHMP4A. Supporting Evidence: PMID:22724069 Borealin interacts directly with the Snf7 components of ESCRT-III PMID:22724069 controls abscission timing through inhibition of ESCRT-III Snf7 polymerization UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0071985 multivesicular body sorting pathway | IEA GO_REF:0000117 | ACCEPT | Summary: Supported core CHMP4A ESCRT-III/MVB membrane-remodeling annotation: multivesicular body sorting pathway. Reason: CHMP4A is a core ESCRT-III/Snf7-family subunit that polymerizes on endosomal and MVB membranes to promote membrane budding/fission and MVB cargo sorting. Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:18209100 Multivesicular bodies (MVBs) are mid-stage endosomes that contain intraluminal vesicles UniProt:Q9BY43 Cytoplasmic vesicle membrane. Late endosome membrane UniProt:Q9BY43 Membrane-associated |
| GO:0097352 autophagosome maturation | IEA GO_REF:0000117 | ACCEPT | Summary: Supported PN-relevant autophagy/endolysosomal CHMP4A context: autophagosome maturation. Reason: CHMP4A/Snf7-1 depletion causes autophagosome accumulation in human neurons, and ESCRT/MVB function is required for autophagic degradation; this supports retaining autophagy and autophagosome-context annotations without claiming CHMP4A alone was directly assayed as the phagophore-sealing effector. Supporting Evidence: PMID:21975012 SiRNA knockdown of hSnf7-1 in human neurons leads to autophagosome accumulation PMID:17984323 Functional multivesicular bodies are required for autophagic clearance UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:1901673 regulation of mitotic spindle assembly | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: regulation of mitotic spindle assembly. Reason: Cytokinesis/midbody and spindle annotations are supported ESCRT output contexts, but they are not the central endolysosomal/proteostasis function emphasized for CHMP4A. Supporting Evidence: PMID:22724069 Borealin interacts directly with the Snf7 components of ESCRT-III PMID:22724069 controls abscission timing through inhibition of ESCRT-III Snf7 polymerization UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:1902774 late endosome to lysosome transport | IEA GO_REF:0000117 | ACCEPT | Summary: Supported PN-relevant autophagy/endolysosomal CHMP4A context: late endosome to lysosome transport. Reason: CHMP4A/Snf7-1 depletion causes autophagosome accumulation in human neurons, and ESCRT/MVB function is required for autophagic degradation; this supports retaining autophagy and autophagosome-context annotations without claiming CHMP4A alone was directly assayed as the phagophore-sealing effector. Supporting Evidence: PMID:21975012 SiRNA knockdown of hSnf7-1 in human neurons leads to autophagosome accumulation PMID:17984323 Functional multivesicular bodies are required for autophagic clearance UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:1904930 amphisome membrane | IEA GO_REF:0000117 | ACCEPT | Summary: Supported PN-relevant autophagy/endolysosomal CHMP4A context: amphisome membrane. Reason: CHMP4A/Snf7-1 depletion causes autophagosome accumulation in human neurons, and ESCRT/MVB function is required for autophagic degradation; this supports retaining autophagy and autophagosome-context annotations without claiming CHMP4A alone was directly assayed as the phagophore-sealing effector. Supporting Evidence: PMID:21975012 SiRNA knockdown of hSnf7-1 in human neurons leads to autophagosome accumulation PMID:17984323 Functional multivesicular bodies are required for autophagic clearance UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0005515 protein binding | IPI PMID:16189514 Towards a proteome-scale map of the human protein-protein in... | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for CHMP4A: protein binding. Reason: CHMP4A has real ESCRT, ALIX, Borealin, VPS4, and high-throughput interactions, but the curated function should describe ESCRT-III polymerization, membrane bending/fission, and specific ESCRT-complex contexts rather than plain protein binding. Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs PMID:18511562 Bro1 domain of ALIX binds specifically to C-terminal residues of the human CHMP4 proteins (CHMP4A-C) PMID:18209100 assemble into regular approximately 5-nm filaments that curve and self-associate PMID:18209100 proteins hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) |
| GO:0005515 protein binding | IPI PMID:16730941 A systematic analysis of human CHMP protein interactions: ad... | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for CHMP4A: protein binding. Reason: CHMP4A has real ESCRT, ALIX, Borealin, VPS4, and high-throughput interactions, but the curated function should describe ESCRT-III polymerization, membrane bending/fission, and specific ESCRT-complex contexts rather than plain protein binding. Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs PMID:18511562 Bro1 domain of ALIX binds specifically to C-terminal residues of the human CHMP4 proteins (CHMP4A-C) PMID:18209100 assemble into regular approximately 5-nm filaments that curve and self-associate PMID:18209100 proteins hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) |
| GO:0005515 protein binding | IPI PMID:17350572 Structural and biochemical studies of ALIX/AIP1 and its role... | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for CHMP4A: protein binding. Reason: CHMP4A has real ESCRT, ALIX, Borealin, VPS4, and high-throughput interactions, but the curated function should describe ESCRT-III polymerization, membrane bending/fission, and specific ESCRT-complex contexts rather than plain protein binding. Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs PMID:18511562 Bro1 domain of ALIX binds specifically to C-terminal residues of the human CHMP4 proteins (CHMP4A-C) PMID:18209100 assemble into regular approximately 5-nm filaments that curve and self-associate PMID:18209100 proteins hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) |
| GO:0005515 protein binding | IPI PMID:18511562 ALIX-CHMP4 interactions in the human ESCRT pathway. | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for CHMP4A: protein binding. Reason: CHMP4A has real ESCRT, ALIX, Borealin, VPS4, and high-throughput interactions, but the curated function should describe ESCRT-III polymerization, membrane bending/fission, and specific ESCRT-complex contexts rather than plain protein binding. Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs PMID:18511562 Bro1 domain of ALIX binds specifically to C-terminal residues of the human CHMP4 proteins (CHMP4A-C) PMID:18209100 assemble into regular approximately 5-nm filaments that curve and self-associate PMID:18209100 proteins hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for CHMP4A: protein binding. Reason: CHMP4A has real ESCRT, ALIX, Borealin, VPS4, and high-throughput interactions, but the curated function should describe ESCRT-III polymerization, membrane bending/fission, and specific ESCRT-complex contexts rather than plain protein binding. Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs PMID:18511562 Bro1 domain of ALIX binds specifically to C-terminal residues of the human CHMP4 proteins (CHMP4A-C) PMID:18209100 assemble into regular approximately 5-nm filaments that curve and self-associate PMID:18209100 proteins hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) |
| GO:0005515 protein binding | IPI PMID:31515488 Extensive disruption of protein interactions by genetic vari... | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for CHMP4A: protein binding. Reason: CHMP4A has real ESCRT, ALIX, Borealin, VPS4, and high-throughput interactions, but the curated function should describe ESCRT-III polymerization, membrane bending/fission, and specific ESCRT-complex contexts rather than plain protein binding. Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs PMID:18511562 Bro1 domain of ALIX binds specifically to C-terminal residues of the human CHMP4 proteins (CHMP4A-C) PMID:18209100 assemble into regular approximately 5-nm filaments that curve and self-associate PMID:18209100 proteins hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) |
| GO:0000421 autophagosome membrane | IDA PMID:17984323 Functional multivesicular bodies are required for autophagic... | ACCEPT | Summary: Supported PN-relevant autophagy/endolysosomal CHMP4A context: autophagosome membrane. Reason: CHMP4A/Snf7-1 depletion causes autophagosome accumulation in human neurons, and ESCRT/MVB function is required for autophagic degradation; this supports retaining autophagy and autophagosome-context annotations without claiming CHMP4A alone was directly assayed as the phagophore-sealing effector. Supporting Evidence: PMID:21975012 SiRNA knockdown of hSnf7-1 in human neurons leads to autophagosome accumulation PMID:17984323 Functional multivesicular bodies are required for autophagic clearance UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0000776 kinetochore | IDA PMID:26040712 Spastin and ESCRT-III coordinate mitotic spindle disassembly... | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: kinetochore. Reason: Cytokinesis/midbody and spindle annotations are supported ESCRT output contexts, but they are not the central endolysosomal/proteostasis function emphasized for CHMP4A. Supporting Evidence: PMID:22724069 Borealin interacts directly with the Snf7 components of ESCRT-III PMID:22724069 controls abscission timing through inhibition of ESCRT-III Snf7 polymerization UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0000815 ESCRT III complex | NAS PMID:36107470 Comprehensive analysis of the human ESCRT-III-MIT domain int... | ACCEPT | Summary: Supported core CHMP4A ESCRT-III/MVB membrane-remodeling annotation: ESCRT III complex. Reason: CHMP4A is a core ESCRT-III/Snf7-family subunit that polymerizes on endosomal and MVB membranes to promote membrane budding/fission and MVB cargo sorting. Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:18209100 Multivesicular bodies (MVBs) are mid-stage endosomes that contain intraluminal vesicles UniProt:Q9BY43 Cytoplasmic vesicle membrane. Late endosome membrane UniProt:Q9BY43 Membrane-associated |
| GO:0001778 plasma membrane repair | IDA PMID:24482116 ESCRT machinery is required for plasma membrane repair. | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: plasma membrane repair. Reason: Plasma membrane repair is a valid ESCRT output context, but the core CHMP4A function remains ESCRT-III membrane remodeling in endosomal/MVB and related compartments. Supporting Evidence: PMID:24482116 ESCRT proteins were recruited within seconds to plasma membrane wounds PMID:24482116 repair of certain wounds is ensured by ESCRT-mediated extracellular shedding of wounded portions UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0005643 nuclear pore | IDA PMID:26040713 ESCRT-III controls nuclear envelope reformation. | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: nuclear pore. Reason: Nuclear envelope or nuclear pore repair/reassembly is a supported ESCRT-III context but is secondary to CHMP4A core ESCRT-III membrane-remodeling function. Supporting Evidence: PMID:26040713 ESCRT-III controls nuclear envelope reformation UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0005765 lysosomal membrane | IDA PMID:17984323 Functional multivesicular bodies are required for autophagic... | ACCEPT | Summary: Supported PN-relevant autophagy/endolysosomal CHMP4A context: lysosomal membrane. Reason: CHMP4A/Snf7-1 depletion causes autophagosome accumulation in human neurons, and ESCRT/MVB function is required for autophagic degradation; this supports retaining autophagy and autophagosome-context annotations without claiming CHMP4A alone was directly assayed as the phagophore-sealing effector. Supporting Evidence: PMID:21975012 SiRNA knockdown of hSnf7-1 in human neurons leads to autophagosome accumulation PMID:17984323 Functional multivesicular bodies are required for autophagic clearance UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0005828 kinetochore microtubule | IDA PMID:26040712 Spastin and ESCRT-III coordinate mitotic spindle disassembly... | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: kinetochore microtubule. Reason: Cytokinesis/midbody and spindle annotations are supported ESCRT output contexts, but they are not the central endolysosomal/proteostasis function emphasized for CHMP4A. Supporting Evidence: PMID:22724069 Borealin interacts directly with the Snf7 components of ESCRT-III PMID:22724069 controls abscission timing through inhibition of ESCRT-III Snf7 polymerization UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0005886 plasma membrane | IDA PMID:24878737 Structure of cellular ESCRT-III spirals and their relationsh... | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: plasma membrane. Reason: This location or output context is supported or plausible for CHMP4A but is broad or secondary relative to its core ESCRT-III MVB/endosomal membrane-remodeling role. Supporting Evidence: UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks UniProt:Q9BY43 Cytoplasmic vesicle membrane. Late endosome membrane UniProt:Q9BY43 Membrane-associated |
| GO:0006914 autophagy | IMP PMID:17984323 Functional multivesicular bodies are required for autophagic... | ACCEPT | Summary: Supported PN-relevant autophagy/endolysosomal CHMP4A context: autophagy. Reason: CHMP4A/Snf7-1 depletion causes autophagosome accumulation in human neurons, and ESCRT/MVB function is required for autophagic degradation; this supports retaining autophagy and autophagosome-context annotations without claiming CHMP4A alone was directly assayed as the phagophore-sealing effector. Supporting Evidence: PMID:21975012 SiRNA knockdown of hSnf7-1 in human neurons leads to autophagosome accumulation PMID:17984323 Functional multivesicular bodies are required for autophagic clearance UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0006997 nucleus organization | IMP PMID:20616062 Human ESCRT-III and VPS4 proteins are required for centrosom... | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: nucleus organization. Reason: Nuclear envelope or nuclear pore repair/reassembly is a supported ESCRT-III context but is secondary to CHMP4A core ESCRT-III membrane-remodeling function. Supporting Evidence: PMID:26040713 ESCRT-III controls nuclear envelope reformation UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0007080 mitotic metaphase chromosome alignment | IMP PMID:20616062 Human ESCRT-III and VPS4 proteins are required for centrosom... | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: mitotic metaphase chromosome alignment. Reason: Cytokinesis/midbody and spindle annotations are supported ESCRT output contexts, but they are not the central endolysosomal/proteostasis function emphasized for CHMP4A. Supporting Evidence: PMID:22724069 Borealin interacts directly with the Snf7 components of ESCRT-III PMID:22724069 controls abscission timing through inhibition of ESCRT-III Snf7 polymerization UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0030496 midbody | IDA PMID:26040712 Spastin and ESCRT-III coordinate mitotic spindle disassembly... | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: midbody. Reason: Cytokinesis/midbody and spindle annotations are supported ESCRT output contexts, but they are not the central endolysosomal/proteostasis function emphasized for CHMP4A. Supporting Evidence: PMID:22724069 Borealin interacts directly with the Snf7 components of ESCRT-III PMID:22724069 controls abscission timing through inhibition of ESCRT-III Snf7 polymerization UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0031468 nuclear membrane reassembly | IMP PMID:26040713 ESCRT-III controls nuclear envelope reformation. | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: nuclear membrane reassembly. Reason: Nuclear envelope or nuclear pore repair/reassembly is a supported ESCRT-III context but is secondary to CHMP4A core ESCRT-III membrane-remodeling function. Supporting Evidence: PMID:26040713 ESCRT-III controls nuclear envelope reformation UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0032585 multivesicular body membrane | IDA PMID:16554368 The ESCRT-III subunit hVps24 is required for degradation but... | ACCEPT | Summary: Supported core CHMP4A ESCRT-III/MVB membrane-remodeling annotation: multivesicular body membrane. Reason: CHMP4A is a core ESCRT-III/Snf7-family subunit that polymerizes on endosomal and MVB membranes to promote membrane budding/fission and MVB cargo sorting. Supporting Evidence: UniProt:Q9BY43 Cytoplasmic vesicle membrane. Late endosome membrane UniProt:Q9BY43 Membrane-associated PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:18209100 Multivesicular bodies (MVBs) are mid-stage endosomes that contain intraluminal vesicles |
| GO:0036258 multivesicular body assembly | NAS PMID:16505166 Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a... | ACCEPT | Summary: Supported core CHMP4A ESCRT-III/MVB membrane-remodeling annotation: multivesicular body assembly. Reason: CHMP4A is a core ESCRT-III/Snf7-family subunit that polymerizes on endosomal and MVB membranes to promote membrane budding/fission and MVB cargo sorting. Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:18209100 Multivesicular bodies (MVBs) are mid-stage endosomes that contain intraluminal vesicles UniProt:Q9BY43 Cytoplasmic vesicle membrane. Late endosome membrane UniProt:Q9BY43 Membrane-associated |
| GO:0039702 viral budding via host ESCRT complex | IDA PMID:24878737 Structure of cellular ESCRT-III spirals and their relationsh... | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: viral budding via host ESCRT complex. Reason: Viral budding is directly supported as ESCRT hijacking but is not an endogenous core CHMP4A proteostasis function. Supporting Evidence: PMID:18511562 ALIX protein must bind and recruit CHMP4 subunits of the ESCRT-III complex PMID:24878737 Structure of cellular ESCRT-III spirals and their relationship to HIV budding UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway | IDA PMID:17984323 Functional multivesicular bodies are required for autophagic... | ACCEPT | Summary: Supported core CHMP4A ESCRT-III/MVB membrane-remodeling annotation: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway. Reason: CHMP4A is a core ESCRT-III/Snf7-family subunit that polymerizes on endosomal and MVB membranes to promote membrane budding/fission and MVB cargo sorting. Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:18209100 Multivesicular bodies (MVBs) are mid-stage endosomes that contain intraluminal vesicles UniProt:Q9BY43 Cytoplasmic vesicle membrane. Late endosome membrane UniProt:Q9BY43 Membrane-associated |
| GO:0046761 viral budding from plasma membrane | IDA PMID:24878737 Structure of cellular ESCRT-III spirals and their relationsh... | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: viral budding from plasma membrane. Reason: Viral budding is directly supported as ESCRT hijacking but is not an endogenous core CHMP4A proteostasis function. Supporting Evidence: PMID:18511562 ALIX protein must bind and recruit CHMP4 subunits of the ESCRT-III complex PMID:24878737 Structure of cellular ESCRT-III spirals and their relationship to HIV budding UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0051469 vesicle fusion with vacuole | NAS PMID:16505166 Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a... | MODIFY | Summary: vesicle fusion with vacuole overstates a direct vesicle-fusion role for CHMP4A. Reason: CHMP4A is an ESCRT-III membrane-remodeling subunit in MVB/endolysosomal trafficking; cached evidence supports late endosome/MVB to lysosome traffic more directly than CHMP4A as a fusion effector. Proposed replacements: late endosome to lysosome transport Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks |
| GO:0061763 multivesicular body-lysosome fusion | NAS PMID:16505166 Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a... | MODIFY | Summary: multivesicular body-lysosome fusion overstates a direct vesicle-fusion role for CHMP4A. Reason: CHMP4A is an ESCRT-III membrane-remodeling subunit in MVB/endolysosomal trafficking; cached evidence supports late endosome/MVB to lysosome traffic more directly than CHMP4A as a fusion effector. Proposed replacements: late endosome to lysosome transport Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks |
| GO:0061952 midbody abscission | IMP PMID:20616062 Human ESCRT-III and VPS4 proteins are required for centrosom... | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: midbody abscission. Reason: Cytokinesis/midbody and spindle annotations are supported ESCRT output contexts, but they are not the central endolysosomal/proteostasis function emphasized for CHMP4A. Supporting Evidence: PMID:22724069 Borealin interacts directly with the Snf7 components of ESCRT-III PMID:22724069 controls abscission timing through inhibition of ESCRT-III Snf7 polymerization UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0071985 multivesicular body sorting pathway | IDA PMID:16554368 The ESCRT-III subunit hVps24 is required for degradation but... | ACCEPT | Summary: Supported core CHMP4A ESCRT-III/MVB membrane-remodeling annotation: multivesicular body sorting pathway. Reason: CHMP4A is a core ESCRT-III/Snf7-family subunit that polymerizes on endosomal and MVB membranes to promote membrane budding/fission and MVB cargo sorting. Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:18209100 Multivesicular bodies (MVBs) are mid-stage endosomes that contain intraluminal vesicles UniProt:Q9BY43 Cytoplasmic vesicle membrane. Late endosome membrane UniProt:Q9BY43 Membrane-associated |
| GO:0090148 membrane fission | NAS PMID:19234443 Membrane scission by the ESCRT-III complex. | ACCEPT | Summary: Supported core CHMP4A ESCRT-III/MVB membrane-remodeling annotation: membrane fission. Reason: CHMP4A is a core ESCRT-III/Snf7-family subunit that polymerizes on endosomal and MVB membranes to promote membrane budding/fission and MVB cargo sorting. Supporting Evidence: UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:18209100 assemble into regular approximately 5-nm filaments that curve and self-associate PMID:18209100 proteins hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) |
| GO:0097352 autophagosome maturation | IMP PMID:17984323 Functional multivesicular bodies are required for autophagic... | ACCEPT | Summary: Supported PN-relevant autophagy/endolysosomal CHMP4A context: autophagosome maturation. Reason: CHMP4A/Snf7-1 depletion causes autophagosome accumulation in human neurons, and ESCRT/MVB function is required for autophagic degradation; this supports retaining autophagy and autophagosome-context annotations without claiming CHMP4A alone was directly assayed as the phagophore-sealing effector. Supporting Evidence: PMID:21975012 SiRNA knockdown of hSnf7-1 in human neurons leads to autophagosome accumulation PMID:17984323 Functional multivesicular bodies are required for autophagic clearance UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:1901673 regulation of mitotic spindle assembly | IMP PMID:20616062 Human ESCRT-III and VPS4 proteins are required for centrosom... | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: regulation of mitotic spindle assembly. Reason: Cytokinesis/midbody and spindle annotations are supported ESCRT output contexts, but they are not the central endolysosomal/proteostasis function emphasized for CHMP4A. Supporting Evidence: PMID:22724069 Borealin interacts directly with the Snf7 components of ESCRT-III PMID:22724069 controls abscission timing through inhibition of ESCRT-III Snf7 polymerization UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:1902774 late endosome to lysosome transport | IMP PMID:17984323 Functional multivesicular bodies are required for autophagic... | ACCEPT | Summary: Supported PN-relevant autophagy/endolysosomal CHMP4A context: late endosome to lysosome transport. Reason: CHMP4A/Snf7-1 depletion causes autophagosome accumulation in human neurons, and ESCRT/MVB function is required for autophagic degradation; this supports retaining autophagy and autophagosome-context annotations without claiming CHMP4A alone was directly assayed as the phagophore-sealing effector. Supporting Evidence: PMID:21975012 SiRNA knockdown of hSnf7-1 in human neurons leads to autophagosome accumulation PMID:17984323 Functional multivesicular bodies are required for autophagic clearance UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:1904930 amphisome membrane | IDA PMID:17984323 Functional multivesicular bodies are required for autophagic... | ACCEPT | Summary: Supported PN-relevant autophagy/endolysosomal CHMP4A context: amphisome membrane. Reason: CHMP4A/Snf7-1 depletion causes autophagosome accumulation in human neurons, and ESCRT/MVB function is required for autophagic degradation; this supports retaining autophagy and autophagosome-context annotations without claiming CHMP4A alone was directly assayed as the phagophore-sealing effector. Supporting Evidence: PMID:21975012 SiRNA knockdown of hSnf7-1 in human neurons leads to autophagosome accumulation PMID:17984323 Functional multivesicular bodies are required for autophagic clearance UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0050877 nervous system process | IMP PMID:21975012 ESCRT-III subunits Snf7-1 and Snf7-2 differentially regulate... | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: nervous system process. Reason: The neuron paper supports CHMP4A-dependent neuronal survival and cargo turnover, but nervous system process is a broad phenotype rather than CHMP4A core molecular/cellular function. Supporting Evidence: PMID:21975012 hSnf7-1 and hSnf7-2 are required for the survival of human neurons PMID:21975012 hSnf7-1 and hSnf7-2 may have preferred interacting partners to form distinct ESCRT-III with different cellular functions |
| GO:0071985 multivesicular body sorting pathway | IMP PMID:21975012 ESCRT-III subunits Snf7-1 and Snf7-2 differentially regulate... | ACCEPT | Summary: Supported core CHMP4A ESCRT-III/MVB membrane-remodeling annotation: multivesicular body sorting pathway. Reason: CHMP4A is a core ESCRT-III/Snf7-family subunit that polymerizes on endosomal and MVB membranes to promote membrane budding/fission and MVB cargo sorting. Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:18209100 Multivesicular bodies (MVBs) are mid-stage endosomes that contain intraluminal vesicles UniProt:Q9BY43 Cytoplasmic vesicle membrane. Late endosome membrane UniProt:Q9BY43 Membrane-associated |
| GO:0000815 ESCRT III complex | IDA PMID:18209100 Plasma membrane deformation by circular arrays of ESCRT-III ... | ACCEPT | Summary: Supported core CHMP4A ESCRT-III/MVB membrane-remodeling annotation: ESCRT III complex. Reason: CHMP4A is a core ESCRT-III/Snf7-family subunit that polymerizes on endosomal and MVB membranes to promote membrane budding/fission and MVB cargo sorting. Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:18209100 Multivesicular bodies (MVBs) are mid-stage endosomes that contain intraluminal vesicles UniProt:Q9BY43 Cytoplasmic vesicle membrane. Late endosome membrane UniProt:Q9BY43 Membrane-associated |
| GO:0042802 identical protein binding | IPI PMID:18209100 Plasma membrane deformation by circular arrays of ESCRT-III ... | MODIFY | Summary: identical protein binding captures CHMP4A self-association but is less accurate than ESCRT-III polymerization and membrane bending. Reason: CHMP4A forms curved ESCRT-III filaments/polymers that bend membranes; homodimer or identical-protein binding terms are weaker molecular descriptions. Proposed replacements: protein polymerization membrane bending activity Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks |
| GO:0051258 protein polymerization | IMP PMID:18209100 Plasma membrane deformation by circular arrays of ESCRT-III ... | ACCEPT | Summary: Supported core CHMP4A ESCRT-III/MVB membrane-remodeling annotation: protein polymerization. Reason: CHMP4A is a core ESCRT-III/Snf7-family subunit that polymerizes on endosomal and MVB membranes to promote membrane budding/fission and MVB cargo sorting. Supporting Evidence: PMID:18209100 assemble into regular approximately 5-nm filaments that curve and self-associate PMID:18209100 proteins hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks |
| GO:0005515 protein binding | IPI PMID:18641129 Differential requirements for Alix and ESCRT-III in cytokine... | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for CHMP4A: protein binding. Reason: CHMP4A has real ESCRT, ALIX, Borealin, VPS4, and high-throughput interactions, but the curated function should describe ESCRT-III polymerization, membrane bending/fission, and specific ESCRT-complex contexts rather than plain protein binding. Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs PMID:18511562 Bro1 domain of ALIX binds specifically to C-terminal residues of the human CHMP4 proteins (CHMP4A-C) PMID:18209100 assemble into regular approximately 5-nm filaments that curve and self-associate PMID:18209100 proteins hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) |
| GO:0016236 macroautophagy | TAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a... | ACCEPT | Summary: Supported PN-relevant autophagy/endolysosomal CHMP4A context: macroautophagy. Reason: CHMP4A/Snf7-1 depletion causes autophagosome accumulation in human neurons, and ESCRT/MVB function is required for autophagic degradation; this supports retaining autophagy and autophagosome-context annotations without claiming CHMP4A alone was directly assayed as the phagophore-sealing effector. Supporting Evidence: PMID:21975012 SiRNA knockdown of hSnf7-1 in human neurons leads to autophagosome accumulation PMID:17984323 Functional multivesicular bodies are required for autophagic clearance UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0000815 ESCRT III complex | TAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a... | ACCEPT | Summary: Supported core CHMP4A ESCRT-III/MVB membrane-remodeling annotation: ESCRT III complex. Reason: CHMP4A is a core ESCRT-III/Snf7-family subunit that polymerizes on endosomal and MVB membranes to promote membrane budding/fission and MVB cargo sorting. Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:18209100 Multivesicular bodies (MVBs) are mid-stage endosomes that contain intraluminal vesicles UniProt:Q9BY43 Cytoplasmic vesicle membrane. Late endosome membrane UniProt:Q9BY43 Membrane-associated |
| GO:0036258 multivesicular body assembly | TAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a... | ACCEPT | Summary: Supported core CHMP4A ESCRT-III/MVB membrane-remodeling annotation: multivesicular body assembly. Reason: CHMP4A is a core ESCRT-III/Snf7-family subunit that polymerizes on endosomal and MVB membranes to promote membrane budding/fission and MVB cargo sorting. Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:18209100 Multivesicular bodies (MVBs) are mid-stage endosomes that contain intraluminal vesicles UniProt:Q9BY43 Cytoplasmic vesicle membrane. Late endosome membrane UniProt:Q9BY43 Membrane-associated |
| GO:0039702 viral budding via host ESCRT complex | TAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a... | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: viral budding via host ESCRT complex. Reason: Viral budding is directly supported as ESCRT hijacking but is not an endogenous core CHMP4A proteostasis function. Supporting Evidence: PMID:18511562 ALIX protein must bind and recruit CHMP4 subunits of the ESCRT-III complex PMID:24878737 Structure of cellular ESCRT-III spirals and their relationship to HIV budding UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0000815 ESCRT III complex | IDA PMID:24878737 Structure of cellular ESCRT-III spirals and their relationsh... | ACCEPT | Summary: Supported core CHMP4A ESCRT-III/MVB membrane-remodeling annotation: ESCRT III complex. Reason: CHMP4A is a core ESCRT-III/Snf7-family subunit that polymerizes on endosomal and MVB membranes to promote membrane budding/fission and MVB cargo sorting. Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:18209100 Multivesicular bodies (MVBs) are mid-stage endosomes that contain intraluminal vesicles UniProt:Q9BY43 Cytoplasmic vesicle membrane. Late endosome membrane UniProt:Q9BY43 Membrane-associated |
| GO:0005737 cytoplasm | IDA PMID:24878737 Structure of cellular ESCRT-III spirals and their relationsh... | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: cytoplasm. Reason: This location or output context is supported or plausible for CHMP4A but is broad or secondary relative to its core ESCRT-III MVB/endosomal membrane-remodeling role. Supporting Evidence: PMID:21975012 Both hSnf7-1 and hSnf7-2 were expressed in human postmitotic neurons and seemed to be present in both the nucleus and cytoplasm |
| GO:0005768 endosome | IDA PMID:24878737 Structure of cellular ESCRT-III spirals and their relationsh... | ACCEPT | Summary: Supported core CHMP4A ESCRT-III/MVB membrane-remodeling annotation: endosome. Reason: CHMP4A is a core ESCRT-III/Snf7-family subunit that polymerizes on endosomal and MVB membranes to promote membrane budding/fission and MVB cargo sorting. Supporting Evidence: UniProt:Q9BY43 Cytoplasmic vesicle membrane. Late endosome membrane UniProt:Q9BY43 Membrane-associated PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:18209100 Multivesicular bodies (MVBs) are mid-stage endosomes that contain intraluminal vesicles |
| GO:0005886 plasma membrane | IDA PMID:24878737 Structure of cellular ESCRT-III spirals and their relationsh... | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: plasma membrane. Reason: This location or output context is supported or plausible for CHMP4A but is broad or secondary relative to its core ESCRT-III MVB/endosomal membrane-remodeling role. Supporting Evidence: UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks UniProt:Q9BY43 Cytoplasmic vesicle membrane. Late endosome membrane UniProt:Q9BY43 Membrane-associated |
| GO:0010324 membrane invagination | IMP PMID:24878737 Structure of cellular ESCRT-III spirals and their relationsh... | ACCEPT | Summary: Supported core CHMP4A ESCRT-III/MVB membrane-remodeling annotation: membrane invagination. Reason: CHMP4A is a core ESCRT-III/Snf7-family subunit that polymerizes on endosomal and MVB membranes to promote membrane budding/fission and MVB cargo sorting. Supporting Evidence: UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:18209100 assemble into regular approximately 5-nm filaments that curve and self-associate PMID:18209100 proteins hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) |
| GO:0030117 membrane coat | IMP PMID:24878737 Structure of cellular ESCRT-III spirals and their relationsh... | ACCEPT | Summary: Supported core CHMP4A ESCRT-III/MVB membrane-remodeling annotation: membrane coat. Reason: CHMP4A is a core ESCRT-III/Snf7-family subunit that polymerizes on endosomal and MVB membranes to promote membrane budding/fission and MVB cargo sorting. Supporting Evidence: UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:18209100 assemble into regular approximately 5-nm filaments that curve and self-associate PMID:18209100 proteins hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) |
| GO:0051258 protein polymerization | IMP PMID:24878737 Structure of cellular ESCRT-III spirals and their relationsh... | ACCEPT | Summary: Supported core CHMP4A ESCRT-III/MVB membrane-remodeling annotation: protein polymerization. Reason: CHMP4A is a core ESCRT-III/Snf7-family subunit that polymerizes on endosomal and MVB membranes to promote membrane budding/fission and MVB cargo sorting. Supporting Evidence: PMID:18209100 assemble into regular approximately 5-nm filaments that curve and self-associate PMID:18209100 proteins hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks |
| GO:0039702 viral budding via host ESCRT complex | IGI PMID:24107264 ESCRT requirements for EIAV budding. | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: viral budding via host ESCRT complex. Reason: Viral budding is directly supported as ESCRT hijacking but is not an endogenous core CHMP4A proteostasis function. Supporting Evidence: PMID:18511562 ALIX protein must bind and recruit CHMP4 subunits of the ESCRT-III complex PMID:24878737 Structure of cellular ESCRT-III spirals and their relationship to HIV budding UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0005515 protein binding | IPI PMID:14519844 Divergent retroviral late-budding domains recruit vacuolar p... | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for CHMP4A: protein binding. Reason: CHMP4A has real ESCRT, ALIX, Borealin, VPS4, and high-throughput interactions, but the curated function should describe ESCRT-III polymerization, membrane bending/fission, and specific ESCRT-complex contexts rather than plain protein binding. Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs PMID:18511562 Bro1 domain of ALIX binds specifically to C-terminal residues of the human CHMP4 proteins (CHMP4A-C) PMID:18209100 assemble into regular approximately 5-nm filaments that curve and self-associate PMID:18209100 proteins hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) |
| GO:0042803 protein homodimerization activity | IPI PMID:14519844 Divergent retroviral late-budding domains recruit vacuolar p... | MODIFY | Summary: protein homodimerization activity captures CHMP4A self-association but is less accurate than ESCRT-III polymerization and membrane bending. Reason: CHMP4A forms curved ESCRT-III filaments/polymers that bend membranes; homodimer or identical-protein binding terms are weaker molecular descriptions. Proposed replacements: protein polymerization membrane bending activity Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks |
| GO:0005515 protein binding | IPI PMID:14505570 The protein network of HIV budding. | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for CHMP4A: protein binding. Reason: CHMP4A has real ESCRT, ALIX, Borealin, VPS4, and high-throughput interactions, but the curated function should describe ESCRT-III polymerization, membrane bending/fission, and specific ESCRT-complex contexts rather than plain protein binding. Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs PMID:18511562 Bro1 domain of ALIX binds specifically to C-terminal residues of the human CHMP4 proteins (CHMP4A-C) PMID:18209100 assemble into regular approximately 5-nm filaments that curve and self-associate PMID:18209100 proteins hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) |
| GO:0000815 ESCRT III complex | IDA PMID:21975012 ESCRT-III subunits Snf7-1 and Snf7-2 differentially regulate... | ACCEPT | Summary: Supported core CHMP4A ESCRT-III/MVB membrane-remodeling annotation: ESCRT III complex. Reason: CHMP4A is a core ESCRT-III/Snf7-family subunit that polymerizes on endosomal and MVB membranes to promote membrane budding/fission and MVB cargo sorting. Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:18209100 Multivesicular bodies (MVBs) are mid-stage endosomes that contain intraluminal vesicles UniProt:Q9BY43 Cytoplasmic vesicle membrane. Late endosome membrane UniProt:Q9BY43 Membrane-associated |
| GO:0005515 protein binding | IPI PMID:21975012 ESCRT-III subunits Snf7-1 and Snf7-2 differentially regulate... | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for CHMP4A: protein binding. Reason: CHMP4A has real ESCRT, ALIX, Borealin, VPS4, and high-throughput interactions, but the curated function should describe ESCRT-III polymerization, membrane bending/fission, and specific ESCRT-complex contexts rather than plain protein binding. Supporting Evidence: UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs PMID:18511562 Bro1 domain of ALIX binds specifically to C-terminal residues of the human CHMP4 proteins (CHMP4A-C) PMID:18209100 assemble into regular approximately 5-nm filaments that curve and self-associate PMID:18209100 proteins hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) |
| GO:0005634 nucleus | IDA PMID:21975012 ESCRT-III subunits Snf7-1 and Snf7-2 differentially regulate... | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: nucleus. Reason: This location or output context is supported or plausible for CHMP4A but is broad or secondary relative to its core ESCRT-III MVB/endosomal membrane-remodeling role. Supporting Evidence: PMID:21975012 Both hSnf7-1 and hSnf7-2 were expressed in human postmitotic neurons and seemed to be present in both the nucleus and cytoplasm |
| GO:0005737 cytoplasm | IDA PMID:21975012 ESCRT-III subunits Snf7-1 and Snf7-2 differentially regulate... | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: cytoplasm. Reason: This location or output context is supported or plausible for CHMP4A but is broad or secondary relative to its core ESCRT-III MVB/endosomal membrane-remodeling role. Supporting Evidence: PMID:21975012 Both hSnf7-1 and hSnf7-2 were expressed in human postmitotic neurons and seemed to be present in both the nucleus and cytoplasm |
| GO:0006620 post-translational protein targeting to endoplasmic reticulum membrane | IMP PMID:21975012 ESCRT-III subunits Snf7-1 and Snf7-2 differentially regulate... | REMOVE | Summary: The cited CHMP4A neuron paper does not support post-translational targeting to the ER membrane. Reason: PMID:21975012 supports CHMP4A/Snf7-1 in neuron survival, ESCRT-III cargo turnover, and autophagosome accumulation; it does not provide evidence that CHMP4A mediates ER membrane targeting. Supporting Evidence: PMID:21975012 hSnf7-1 and hSnf7-2 are required for the survival of human neurons PMID:21975012 hSnf7-1 and hSnf7-2 may have preferred interacting partners to form distinct ESCRT-III with different cellular functions |
| GO:0006900 vesicle budding from membrane | IMP PMID:18209100 Plasma membrane deformation by circular arrays of ESCRT-III ... | ACCEPT | Summary: Supported core CHMP4A ESCRT-III/MVB membrane-remodeling annotation: vesicle budding from membrane. Reason: CHMP4A is a core ESCRT-III/Snf7-family subunit that polymerizes on endosomal and MVB membranes to promote membrane budding/fission and MVB cargo sorting. Supporting Evidence: UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:18209100 assemble into regular approximately 5-nm filaments that curve and self-associate PMID:18209100 proteins hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) |
| GO:0006900 vesicle budding from membrane | IGI PMID:18209100 Plasma membrane deformation by circular arrays of ESCRT-III ... | ACCEPT | Summary: Supported core CHMP4A ESCRT-III/MVB membrane-remodeling annotation: vesicle budding from membrane. Reason: CHMP4A is a core ESCRT-III/Snf7-family subunit that polymerizes on endosomal and MVB membranes to promote membrane budding/fission and MVB cargo sorting. Supporting Evidence: UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:18209100 assemble into regular approximately 5-nm filaments that curve and self-associate PMID:18209100 proteins hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) |
| GO:0009898 cytoplasmic side of plasma membrane | IDA PMID:18209100 Plasma membrane deformation by circular arrays of ESCRT-III ... | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: cytoplasmic side of plasma membrane. Reason: This location or output context is supported or plausible for CHMP4A but is broad or secondary relative to its core ESCRT-III MVB/endosomal membrane-remodeling role. Supporting Evidence: UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks UniProt:Q9BY43 Cytoplasmic vesicle membrane. Late endosome membrane UniProt:Q9BY43 Membrane-associated |
| GO:0051117 ATPase binding | IPI PMID:18209100 Plasma membrane deformation by circular arrays of ESCRT-III ... | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: ATPase binding. Reason: ATPase binding reflects VPS4 engagement with CHMP4A filaments and is useful context, but CHMP4A itself is the ESCRT-III polymerizing membrane-remodeling subunit. Supporting Evidence: PMID:18209100 Binding to a coexpressed adenosine triphosphate hydrolysis-deficient mutant of VPS4B draws these filaments together UniProt:Q9BY43 Interacts with VPS4A |
| GO:0097320 plasma membrane tubulation | IMP PMID:18209100 Plasma membrane deformation by circular arrays of ESCRT-III ... | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: plasma membrane tubulation. Reason: Plasma membrane tubulation is a useful overexpression/model phenotype for CHMP4A filament curvature, not the normal core biological process. Supporting Evidence: UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks UniProt:Q9BY43 Cytoplasmic vesicle membrane. Late endosome membrane UniProt:Q9BY43 Membrane-associated |
| GO:0097320 plasma membrane tubulation | IGI PMID:18209100 Plasma membrane deformation by circular arrays of ESCRT-III ... | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: plasma membrane tubulation. Reason: Plasma membrane tubulation is a useful overexpression/model phenotype for CHMP4A filament curvature, not the normal core biological process. Supporting Evidence: UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks UniProt:Q9BY43 Cytoplasmic vesicle membrane. Late endosome membrane UniProt:Q9BY43 Membrane-associated |
| GO:0005829 cytosol | TAS Reactome:R-HSA-3159232 | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: cytosol. Reason: This location or output context is supported or plausible for CHMP4A but is broad or secondary relative to its core ESCRT-III MVB/endosomal membrane-remodeling role. Supporting Evidence: PMID:21975012 Both hSnf7-1 and hSnf7-2 were expressed in human postmitotic neurons and seemed to be present in both the nucleus and cytoplasm |
| GO:0005829 cytosol | TAS Reactome:R-HSA-917693 | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: cytosol. Reason: This location or output context is supported or plausible for CHMP4A but is broad or secondary relative to its core ESCRT-III MVB/endosomal membrane-remodeling role. Supporting Evidence: PMID:21975012 Both hSnf7-1 and hSnf7-2 were expressed in human postmitotic neurons and seemed to be present in both the nucleus and cytoplasm |
| GO:0005829 cytosol | TAS Reactome:R-HSA-917700 | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: cytosol. Reason: This location or output context is supported or plausible for CHMP4A but is broad or secondary relative to its core ESCRT-III MVB/endosomal membrane-remodeling role. Supporting Evidence: PMID:21975012 Both hSnf7-1 and hSnf7-2 were expressed in human postmitotic neurons and seemed to be present in both the nucleus and cytoplasm |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9668389 | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: cytosol. Reason: This location or output context is supported or plausible for CHMP4A but is broad or secondary relative to its core ESCRT-III MVB/endosomal membrane-remodeling role. Supporting Evidence: PMID:21975012 Both hSnf7-1 and hSnf7-2 were expressed in human postmitotic neurons and seemed to be present in both the nucleus and cytoplasm |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9668395 | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: cytosol. Reason: This location or output context is supported or plausible for CHMP4A but is broad or secondary relative to its core ESCRT-III MVB/endosomal membrane-remodeling role. Supporting Evidence: PMID:21975012 Both hSnf7-1 and hSnf7-2 were expressed in human postmitotic neurons and seemed to be present in both the nucleus and cytoplasm |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9668398 | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: cytosol. Reason: This location or output context is supported or plausible for CHMP4A but is broad or secondary relative to its core ESCRT-III MVB/endosomal membrane-remodeling role. Supporting Evidence: PMID:21975012 Both hSnf7-1 and hSnf7-2 were expressed in human postmitotic neurons and seemed to be present in both the nucleus and cytoplasm |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9668405 | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: cytosol. Reason: This location or output context is supported or plausible for CHMP4A but is broad or secondary relative to its core ESCRT-III MVB/endosomal membrane-remodeling role. Supporting Evidence: PMID:21975012 Both hSnf7-1 and hSnf7-2 were expressed in human postmitotic neurons and seemed to be present in both the nucleus and cytoplasm |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9668415 | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: cytosol. Reason: This location or output context is supported or plausible for CHMP4A but is broad or secondary relative to its core ESCRT-III MVB/endosomal membrane-remodeling role. Supporting Evidence: PMID:21975012 Both hSnf7-1 and hSnf7-2 were expressed in human postmitotic neurons and seemed to be present in both the nucleus and cytoplasm |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9668419 | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: cytosol. Reason: This location or output context is supported or plausible for CHMP4A but is broad or secondary relative to its core ESCRT-III MVB/endosomal membrane-remodeling role. Supporting Evidence: PMID:21975012 Both hSnf7-1 and hSnf7-2 were expressed in human postmitotic neurons and seemed to be present in both the nucleus and cytoplasm |
| GO:0030496 midbody | IDA PMID:22724069 The chromosomal passenger complex controls the function of e... | KEEP AS NON CORE | Summary: True or plausible non-core CHMP4A ESCRT context: midbody. Reason: Cytokinesis/midbody and spindle annotations are supported ESCRT output contexts, but they are not the central endolysosomal/proteostasis function emphasized for CHMP4A. Supporting Evidence: PMID:22724069 Borealin interacts directly with the Snf7 components of ESCRT-III PMID:22724069 controls abscission timing through inhibition of ESCRT-III Snf7 polymerization UniProt:Q9BY43 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9BY43 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs |
| GO:0180020 membrane bending activity | IDA PMID:18209100 Plasma membrane deformation by circular arrays of ESCRT-III ... | NEW | Summary: New CHMP4A molecular-function annotation supported by direct ESCRT-III filament/membrane curvature evidence. Reason: PMID:18209100 directly shows CHMP4A/hSnf7-1-containing ESCRT-III filaments promote or stabilize negative membrane curvature and outward budding, making membrane bending activity more informative than generic protein binding. Supporting Evidence: UniProt:Q9BY43 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:18209100 assemble into regular approximately 5-nm filaments that curve and self-associate PMID:18209100 proteins hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) |
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Download this section (compressed HTML)Q: Which CHMP4 paralog, CHMP4A, CHMP4B, or CHMP4C, is the dominant Snf7-family subunit during human phagophore closure and autophagosome maturation?
Q: Should GO annotations distinguish direct CHMP4A membrane-bending/polymer function from broader ESCRT output processes such as viral budding, plasma membrane repair, and nuclear envelope sealing?
Q: Does CHMP4A preferentially pair with CHMP2A in autophagy and endosomal cargo sorting, as suggested by human-neuron cargo turnover assays?
Experiment: Use endogenous CHMP4A tagging and acute CHMP4A depletion/rescue during starvation-induced autophagy to image recruitment to phagophores, autophagosomes, and amphisomes.
Hypothesis: If CHMP4A directly participates in autophagosome maturation or closure, it should be transiently recruited to autophagy membranes and rescue should require its polymerization/membrane-binding region.
Experiment: Compare wild-type CHMP4A with ALIX-binding and C-terminal autoinhibition mutants in MVB cargo sorting, ESCRT spiral formation, plasma membrane repair, and viral budding assays.
Hypothesis: CHMP4A polymerization and partner recruitment requirements differ across endogenous ESCRT outputs and virus-hijacked budding.
Experiment: Biochemically reconstitute CHMP4A-containing human ESCRT-III polymers with CHMP2A/CHMP3/VPS4 and test membrane bending/scission on defined liposomes.
Hypothesis: CHMP4A contributes Snf7-family filament curvature that can drive membrane bending and support reverse-topology scission in a minimal human ESCRT-III module.
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