CHMP4B encodes hSnf7-2, a Snf7-family core subunit of ESCRT-III. Its best-supported function is non-enzymatic ESCRT-III polymerization on endosomal/MVB and related membranes, where CHMP4B-containing filaments bend membranes and support reverse-topology budding/fission for intraluminal vesicle formation, MVB cargo sorting, and endolysosomal degradation. CHMP4B is also an important paralog for human neuronal cargo turnover/autophagosome accumulation, cytokinetic abscission, viral budding, nuclear envelope sealing, plasma membrane repair, and lens transparency, but these contexts should be interpreted through the core ESCRT-III membrane-remodeling mechanism.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005635 nuclear envelope | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: Supported non-core CHMP4B nuclear-envelope/mitotic-exit context: nuclear envelope. Reason: Nuclear envelope sealing/reassembly is a supported ESCRT-III context but is secondary to CHMP4B core ESCRT-III MVB/endosomal membrane remodeling. Supporting Evidence: UniProt:Q9H444 promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase PMID:26040712 ESCRT-III, VPS4 and spastin cooperate to coordinate nuclear envelope sealing and spindle disassembly PMID:26040713 GFP-CHMP4B transiently localises to telophase nuclei |
| GO:0031468 nuclear membrane reassembly | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: Supported non-core CHMP4B nuclear-envelope/mitotic-exit context: nuclear membrane reassembly. Reason: Nuclear envelope sealing/reassembly is a supported ESCRT-III context but is secondary to CHMP4B core ESCRT-III MVB/endosomal membrane remodeling. Supporting Evidence: UniProt:Q9H444 promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase PMID:26040712 ESCRT-III, VPS4 and spastin cooperate to coordinate nuclear envelope sealing and spindle disassembly PMID:26040713 GFP-CHMP4B transiently localises to telophase nuclei |
| GO:0000815 ESCRT III complex | IBA GO_REF:0000033 | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: ESCRT III complex. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0009898 cytoplasmic side of plasma membrane | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: True or plausible broad CHMP4B location: cytoplasmic side of plasma membrane. Reason: This location is compatible with CHMP4B cycling between cytosol and recruited membrane-associated ESCRT-III assemblies, but it is too broad to represent the core function by itself. Supporting Evidence: UniProt:Q9H444 Cytoplasm. Cytosol. Late endosome membrane UniProt:Q9H444 Membrane-associated |
| GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway | IBA GO_REF:0000033 | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: late endosome to vacuole transport via multivesicular body sorting pathway. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0000776 kinetochore | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Supported non-core CHMP4B centrosome/spindle/cell-cycle context: kinetochore. Reason: ESCRT-III/VPS4 perturbation affects centrosome and spindle maintenance and chromosome segregation, but these cell-cycle annotations are secondary to CHMP4B core ESCRT-III membrane-remodeling function. Supporting Evidence: PMID:20616062 depletion of individual ESCRT-III and VPS4 proteins also altered centrosome and spindle pole numbers PMID:20616062 ESCRT-III/VPS4 proteins function at centrosomes to help regulate their maintenance or proliferation and then at midbodies during abscission UniProt:Q9H444 promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase |
| GO:0001778 plasma membrane repair | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Supported non-core CHMP4B plasma-membrane repair context. Reason: Plasma membrane repair is a valid topologically related ESCRT output, but it is secondary to the core MVB/endosomal ESCRT-III function. Supporting Evidence: PMID:24482116 ESCRT proteins were recruited within seconds to plasma membrane wounds PMID:24482116 repair of certain wounds is ensured by ESCRT-mediated extracellular shedding of wounded portions UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities |
| GO:0005635 nuclear envelope | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: Supported non-core CHMP4B nuclear-envelope/mitotic-exit context: nuclear envelope. Reason: Nuclear envelope sealing/reassembly is a supported ESCRT-III context but is secondary to CHMP4B core ESCRT-III MVB/endosomal membrane remodeling. Supporting Evidence: UniProt:Q9H444 promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase PMID:26040712 ESCRT-III, VPS4 and spastin cooperate to coordinate nuclear envelope sealing and spindle disassembly PMID:26040713 GFP-CHMP4B transiently localises to telophase nuclei |
| GO:0005643 nuclear pore | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Supported non-core CHMP4B nuclear-envelope/mitotic-exit context: nuclear pore. Reason: Nuclear envelope sealing/reassembly is a supported ESCRT-III context but is secondary to CHMP4B core ESCRT-III MVB/endosomal membrane remodeling. Supporting Evidence: UniProt:Q9H444 promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase PMID:26040712 ESCRT-III, VPS4 and spastin cooperate to coordinate nuclear envelope sealing and spindle disassembly PMID:26040713 GFP-CHMP4B transiently localises to telophase nuclei |
| GO:0005765 lysosomal membrane | IEA GO_REF:0000117 | ACCEPT | Summary: Supported PN-relevant CHMP4B autophagy/endolysosomal annotation: lysosomal membrane. Reason: Human-neuron hSnf7-2/CHMP4B knockdown causes autophagosome accumulation, and ESCRT/MVB function is required for autophagic clearance; these annotations are retained as PN-relevant endolysosomal/autophagy context. Supporting Evidence: PMID:21975012 SiRNA knockdown of hSnf7-2 in human neurons leads to autophagosome accumulation PMID:21975012 hSnf7-2 and CHMP2B were more abundant than those containing CHMP2A PMID:17984323 Functional multivesicular bodies are required for autophagic clearance PMID:17984323 autophagic degradation is inhibited in cells depleted of ESCRT subunits UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0005828 kinetochore microtubule | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Supported non-core CHMP4B centrosome/spindle/cell-cycle context: kinetochore microtubule. Reason: ESCRT-III/VPS4 perturbation affects centrosome and spindle maintenance and chromosome segregation, but these cell-cycle annotations are secondary to CHMP4B core ESCRT-III membrane-remodeling function. Supporting Evidence: PMID:20616062 depletion of individual ESCRT-III and VPS4 proteins also altered centrosome and spindle pole numbers PMID:20616062 ESCRT-III/VPS4 proteins function at centrosomes to help regulate their maintenance or proliferation and then at midbodies during abscission UniProt:Q9H444 promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase |
| GO:0005829 cytosol | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: True or plausible broad CHMP4B location: cytosol. Reason: This location is compatible with CHMP4B cycling between cytosol and recruited membrane-associated ESCRT-III assemblies, but it is too broad to represent the core function by itself. Supporting Evidence: UniProt:Q9H444 Cytoplasm. Cytosol. Late endosome membrane UniProt:Q9H444 Membrane-associated |
| GO:0007034 vacuolar transport | IEA GO_REF:0000002 | MODIFY | Summary: CHMP4B annotation should be replaced with a more accurate ESCRT-III term: vacuolar transport. Reason: Multivesicular body sorting is the informative ESCRT-III process; vacuolar transport is too broad for human CHMP4B. Proposed replacements: multivesicular body sorting pathway Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting |
| GO:0007080 mitotic metaphase chromosome alignment | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Supported non-core CHMP4B centrosome/spindle/cell-cycle context: mitotic metaphase chromosome alignment. Reason: ESCRT-III/VPS4 perturbation affects centrosome and spindle maintenance and chromosome segregation, but these cell-cycle annotations are secondary to CHMP4B core ESCRT-III membrane-remodeling function. Supporting Evidence: PMID:20616062 depletion of individual ESCRT-III and VPS4 proteins also altered centrosome and spindle pole numbers PMID:20616062 ESCRT-III/VPS4 proteins function at centrosomes to help regulate their maintenance or proliferation and then at midbodies during abscission UniProt:Q9H444 promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase |
| GO:0030496 midbody | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: Supported non-core CHMP4B cytokinesis/abscission context: midbody. Reason: CHMP4B has strong evidence in ESCRT-dependent cytokinetic abscission, but this is a secondary topologically related ESCRT output rather than the central endosomal/proteostasis function emphasized here. Supporting Evidence: PMID:22422861 CHMP4B was again the sole paralog required PMID:22422861 GFP-CHMP4B localized transiently to the midbody arms immediately PMID:21310966 ESCRT-III co-localized with constriction zones and was required for assembly of 17-nanometer-diameter filaments UniProt:Q9H444 functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis |
| GO:0031468 nuclear membrane reassembly | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Supported non-core CHMP4B nuclear-envelope/mitotic-exit context: nuclear membrane reassembly. Reason: Nuclear envelope sealing/reassembly is a supported ESCRT-III context but is secondary to CHMP4B core ESCRT-III MVB/endosomal membrane remodeling. Supporting Evidence: UniProt:Q9H444 promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase PMID:26040712 ESCRT-III, VPS4 and spastin cooperate to coordinate nuclear envelope sealing and spindle disassembly PMID:26040713 GFP-CHMP4B transiently localises to telophase nuclei |
| GO:0031902 late endosome membrane | IEA GO_REF:0000044 | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: late endosome membrane. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0032585 multivesicular body membrane | IEA GO_REF:0000117 | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: multivesicular body membrane. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0036258 multivesicular body assembly | IEA GO_REF:0000117 | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: multivesicular body assembly. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0039702 viral budding via host ESCRT complex | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Supported non-core CHMP4B viral budding context: viral budding via host ESCRT complex. Reason: CHMP4B is specifically required for ESCRT-dependent HIV/viral budding, but viral budding is a pathogen-hijacked ESCRT output rather than a core host proteostasis function. Supporting Evidence: UniProt:Q9H444 Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release PMID:22422861 only depletion of CHMP4B inhibited this ESCRT-dependent process PMID:24878737 ESCRT machinery along with the AAA+ ATPase Vps4 drive membrane scission |
| GO:0042803 protein homodimerization activity | IEA GO_REF:0000117 | MODIFY | Summary: CHMP4B annotation should be replaced with a more accurate ESCRT-III term: protein homodimerization activity. Reason: Homodimerization is less informative than CHMP4B/Snf7-family polymerization and membrane bending. Proposed replacements: protein polymerization membrane bending activity Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting |
| GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway | IEA GO_REF:0000117 | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0046761 viral budding from plasma membrane | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Supported non-core CHMP4B viral budding context: viral budding from plasma membrane. Reason: CHMP4B is specifically required for ESCRT-dependent HIV/viral budding, but viral budding is a pathogen-hijacked ESCRT output rather than a core host proteostasis function. Supporting Evidence: UniProt:Q9H444 Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release PMID:22422861 only depletion of CHMP4B inhibited this ESCRT-dependent process PMID:24878737 ESCRT machinery along with the AAA+ ATPase Vps4 drive membrane scission |
| GO:0061952 midbody abscission | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Supported non-core CHMP4B cytokinesis/abscission context: midbody abscission. Reason: CHMP4B has strong evidence in ESCRT-dependent cytokinetic abscission, but this is a secondary topologically related ESCRT output rather than the central endosomal/proteostasis function emphasized here. Supporting Evidence: PMID:22422861 CHMP4B was again the sole paralog required PMID:22422861 GFP-CHMP4B localized transiently to the midbody arms immediately PMID:21310966 ESCRT-III co-localized with constriction zones and was required for assembly of 17-nanometer-diameter filaments UniProt:Q9H444 functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis |
| GO:0071985 multivesicular body sorting pathway | IEA GO_REF:0000117 | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: multivesicular body sorting pathway. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0097352 autophagosome maturation | IEA GO_REF:0000117 | ACCEPT | Summary: Supported PN-relevant CHMP4B autophagy/endolysosomal annotation: autophagosome maturation. Reason: Human-neuron hSnf7-2/CHMP4B knockdown causes autophagosome accumulation, and ESCRT/MVB function is required for autophagic clearance; these annotations are retained as PN-relevant endolysosomal/autophagy context. Supporting Evidence: PMID:21975012 SiRNA knockdown of hSnf7-2 in human neurons leads to autophagosome accumulation PMID:21975012 hSnf7-2 and CHMP2B were more abundant than those containing CHMP2A PMID:17984323 Functional multivesicular bodies are required for autophagic clearance PMID:17984323 autophagic degradation is inhibited in cells depleted of ESCRT subunits UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:1901673 regulation of mitotic spindle assembly | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Supported non-core CHMP4B centrosome/spindle/cell-cycle context: regulation of mitotic spindle assembly. Reason: ESCRT-III/VPS4 perturbation affects centrosome and spindle maintenance and chromosome segregation, but these cell-cycle annotations are secondary to CHMP4B core ESCRT-III membrane-remodeling function. Supporting Evidence: PMID:20616062 depletion of individual ESCRT-III and VPS4 proteins also altered centrosome and spindle pole numbers PMID:20616062 ESCRT-III/VPS4 proteins function at centrosomes to help regulate their maintenance or proliferation and then at midbodies during abscission UniProt:Q9H444 promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase |
| GO:1902774 late endosome to lysosome transport | IEA GO_REF:0000117 | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: late endosome to lysosome transport. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:1904930 amphisome membrane | IEA GO_REF:0000117 | ACCEPT | Summary: Supported PN-relevant CHMP4B autophagy/endolysosomal annotation: amphisome membrane. Reason: Human-neuron hSnf7-2/CHMP4B knockdown causes autophagosome accumulation, and ESCRT/MVB function is required for autophagic clearance; these annotations are retained as PN-relevant endolysosomal/autophagy context. Supporting Evidence: PMID:21975012 SiRNA knockdown of hSnf7-2 in human neurons leads to autophagosome accumulation PMID:21975012 hSnf7-2 and CHMP2B were more abundant than those containing CHMP2A PMID:17984323 Functional multivesicular bodies are required for autophagic clearance PMID:17984323 autophagic degradation is inhibited in cells depleted of ESCRT subunits UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0005515 protein binding | IPI PMID:16189514 Towards a proteome-scale map of the human protein-protein in... | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP4B molecular-function annotation: protein binding. Reason: The cited interaction may be real, but protein binding is not informative for CHMP4B; the supported molecular function is ESCRT-III polymerization and membrane bending/scission on endosomal and related membranes. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-notes.md Generic protein binding rows were treated as over-annotated because they do not capture the ESCRT-III polymer/membrane-remodeling function. |
| GO:0005515 protein binding | IPI PMID:16730941 A systematic analysis of human CHMP protein interactions: ad... | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP4B molecular-function annotation: protein binding. Reason: The cited interaction may be real, but protein binding is not informative for CHMP4B; the supported molecular function is ESCRT-III polymerization and membrane bending/scission on endosomal and related membranes. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-notes.md Generic protein binding rows were treated as over-annotated because they do not capture the ESCRT-III polymer/membrane-remodeling function. |
| GO:0005515 protein binding | IPI PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CH... | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP4B molecular-function annotation: protein binding. Reason: The cited interaction may be real, but protein binding is not informative for CHMP4B; the supported molecular function is ESCRT-III polymerization and membrane bending/scission on endosomal and related membranes. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-notes.md Generic protein binding rows were treated as over-annotated because they do not capture the ESCRT-III polymer/membrane-remodeling function. |
| GO:0005515 protein binding | IPI PMID:17174262 HD-PTP and Alix share some membrane-traffic related proteins... | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP4B molecular-function annotation: protein binding. Reason: The cited interaction may be real, but protein binding is not informative for CHMP4B; the supported molecular function is ESCRT-III polymerization and membrane bending/scission on endosomal and related membranes. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-notes.md Generic protein binding rows were treated as over-annotated because they do not capture the ESCRT-III polymer/membrane-remodeling function. |
| GO:0005515 protein binding | IPI PMID:17500595 Huntingtin interacting proteins are genetic modifiers of neu... | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP4B molecular-function annotation: protein binding. Reason: The cited interaction may be real, but protein binding is not informative for CHMP4B; the supported molecular function is ESCRT-III polymerization and membrane bending/scission on endosomal and related membranes. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-notes.md Generic protein binding rows were treated as over-annotated because they do not capture the ESCRT-III polymer/membrane-remodeling function. |
| GO:0005515 protein binding | IPI PMID:17711858 The MIT domain of UBPY constitutes a CHMP binding and endoso... | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP4B molecular-function annotation: protein binding. Reason: The cited interaction may be real, but protein binding is not informative for CHMP4B; the supported molecular function is ESCRT-III polymerization and membrane bending/scission on endosomal and related membranes. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-notes.md Generic protein binding rows were treated as over-annotated because they do not capture the ESCRT-III polymer/membrane-remodeling function. |
| GO:0005515 protein binding | IPI PMID:18434552 The Bro1-related protein HD-PTP/PTPN23 is required for endos... | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP4B molecular-function annotation: protein binding. Reason: The cited interaction may be real, but protein binding is not informative for CHMP4B; the supported molecular function is ESCRT-III polymerization and membrane bending/scission on endosomal and related membranes. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-notes.md Generic protein binding rows were treated as over-annotated because they do not capture the ESCRT-III polymer/membrane-remodeling function. |
| GO:0005515 protein binding | IPI PMID:18511562 ALIX-CHMP4 interactions in the human ESCRT pathway. | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP4B molecular-function annotation: protein binding. Reason: The cited interaction may be real, but protein binding is not informative for CHMP4B; the supported molecular function is ESCRT-III polymerization and membrane bending/scission on endosomal and related membranes. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-notes.md Generic protein binding rows were treated as over-annotated because they do not capture the ESCRT-III polymer/membrane-remodeling function. |
| GO:0005515 protein binding | IPI PMID:19523902 A crescent-shaped ALIX dimer targets ESCRT-III CHMP4 filamen... | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP4B molecular-function annotation: protein binding. Reason: The cited interaction may be real, but protein binding is not informative for CHMP4B; the supported molecular function is ESCRT-III polymerization and membrane bending/scission on endosomal and related membranes. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-notes.md Generic protein binding rows were treated as over-annotated because they do not capture the ESCRT-III polymer/membrane-remodeling function. |
| GO:0005515 protein binding | IPI PMID:21889351 The Phe105 loop of Alix Bro1 domain plays a key role in HIV-... | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP4B molecular-function annotation: protein binding. Reason: The cited interaction may be real, but protein binding is not informative for CHMP4B; the supported molecular function is ESCRT-III polymerization and membrane bending/scission on endosomal and related membranes. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-notes.md Generic protein binding rows were treated as over-annotated because they do not capture the ESCRT-III polymer/membrane-remodeling function. |
| GO:0005515 protein binding | IPI PMID:22484091 Two distinct binding modes define the interaction of Brox wi... | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP4B molecular-function annotation: protein binding. Reason: The cited interaction may be real, but protein binding is not informative for CHMP4B; the supported molecular function is ESCRT-III polymerization and membrane bending/scission on endosomal and related membranes. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-notes.md Generic protein binding rows were treated as over-annotated because they do not capture the ESCRT-III polymer/membrane-remodeling function. |
| GO:0005515 protein binding | IPI PMID:23051622 ESCRT-III CHMP2A and CHMP3 form variable helical polymers in... | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP4B molecular-function annotation: protein binding. Reason: The cited interaction may be real, but protein binding is not informative for CHMP4B; the supported molecular function is ESCRT-III polymerization and membrane bending/scission on endosomal and related membranes. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-notes.md Generic protein binding rows were treated as over-annotated because they do not capture the ESCRT-III polymer/membrane-remodeling function. |
| GO:0005515 protein binding | IPI PMID:26496610 A human interactome in three quantitative dimensions organiz... | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP4B molecular-function annotation: protein binding. Reason: The cited interaction may be real, but protein binding is not informative for CHMP4B; the supported molecular function is ESCRT-III polymerization and membrane bending/scission on endosomal and related membranes. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-notes.md Generic protein binding rows were treated as over-annotated because they do not capture the ESCRT-III polymer/membrane-remodeling function. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP4B molecular-function annotation: protein binding. Reason: The cited interaction may be real, but protein binding is not informative for CHMP4B; the supported molecular function is ESCRT-III polymerization and membrane bending/scission on endosomal and related membranes. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-notes.md Generic protein binding rows were treated as over-annotated because they do not capture the ESCRT-III polymer/membrane-remodeling function. |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP4B molecular-function annotation: protein binding. Reason: The cited interaction may be real, but protein binding is not informative for CHMP4B; the supported molecular function is ESCRT-III polymerization and membrane bending/scission on endosomal and related membranes. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-notes.md Generic protein binding rows were treated as over-annotated because they do not capture the ESCRT-III polymer/membrane-remodeling function. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP4B molecular-function annotation: protein binding. Reason: The cited interaction may be real, but protein binding is not informative for CHMP4B; the supported molecular function is ESCRT-III polymerization and membrane bending/scission on endosomal and related membranes. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-notes.md Generic protein binding rows were treated as over-annotated because they do not capture the ESCRT-III polymer/membrane-remodeling function. |
| GO:0042802 identical protein binding | IPI PMID:16730941 A systematic analysis of human CHMP protein interactions: ad... | MODIFY | Summary: CHMP4B annotation should be replaced with a more accurate ESCRT-III term: identical protein binding. Reason: Identical protein binding is less informative than CHMP4B/Snf7-family polymerization and membrane bending. Proposed replacements: protein polymerization membrane bending activity Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting |
| GO:0042802 identical protein binding | IPI PMID:19523902 A crescent-shaped ALIX dimer targets ESCRT-III CHMP4 filamen... | MODIFY | Summary: CHMP4B annotation should be replaced with a more accurate ESCRT-III term: identical protein binding. Reason: Identical protein binding is less informative than CHMP4B/Snf7-family polymerization and membrane bending. Proposed replacements: protein polymerization membrane bending activity Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting |
| GO:0000421 autophagosome membrane | IDA PMID:17984323 Functional multivesicular bodies are required for autophagic... | ACCEPT | Summary: Supported PN-relevant CHMP4B autophagy/endolysosomal annotation: autophagosome membrane. Reason: Human-neuron hSnf7-2/CHMP4B knockdown causes autophagosome accumulation, and ESCRT/MVB function is required for autophagic clearance; these annotations are retained as PN-relevant endolysosomal/autophagy context. Supporting Evidence: PMID:21975012 SiRNA knockdown of hSnf7-2 in human neurons leads to autophagosome accumulation PMID:21975012 hSnf7-2 and CHMP2B were more abundant than those containing CHMP2A PMID:17984323 Functional multivesicular bodies are required for autophagic clearance PMID:17984323 autophagic degradation is inhibited in cells depleted of ESCRT subunits UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0000776 kinetochore | IDA PMID:26040712 Spastin and ESCRT-III coordinate mitotic spindle disassembly... | KEEP AS NON CORE | Summary: Supported non-core CHMP4B centrosome/spindle/cell-cycle context: kinetochore. Reason: ESCRT-III/VPS4 perturbation affects centrosome and spindle maintenance and chromosome segregation, but these cell-cycle annotations are secondary to CHMP4B core ESCRT-III membrane-remodeling function. Supporting Evidence: PMID:20616062 depletion of individual ESCRT-III and VPS4 proteins also altered centrosome and spindle pole numbers PMID:20616062 ESCRT-III/VPS4 proteins function at centrosomes to help regulate their maintenance or proliferation and then at midbodies during abscission UniProt:Q9H444 promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase |
| GO:0000815 ESCRT III complex | NAS PMID:36107470 Comprehensive analysis of the human ESCRT-III-MIT domain int... | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: ESCRT III complex. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0001778 plasma membrane repair | IDA PMID:24482116 ESCRT machinery is required for plasma membrane repair. | KEEP AS NON CORE | Summary: Supported non-core CHMP4B plasma-membrane repair context. Reason: Plasma membrane repair is a valid topologically related ESCRT output, but it is secondary to the core MVB/endosomal ESCRT-III function. Supporting Evidence: PMID:24482116 ESCRT proteins were recruited within seconds to plasma membrane wounds PMID:24482116 repair of certain wounds is ensured by ESCRT-mediated extracellular shedding of wounded portions UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities |
| GO:0005643 nuclear pore | IDA PMID:26040713 ESCRT-III controls nuclear envelope reformation. | KEEP AS NON CORE | Summary: Supported non-core CHMP4B nuclear-envelope/mitotic-exit context: nuclear pore. Reason: Nuclear envelope sealing/reassembly is a supported ESCRT-III context but is secondary to CHMP4B core ESCRT-III MVB/endosomal membrane remodeling. Supporting Evidence: UniProt:Q9H444 promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase PMID:26040712 ESCRT-III, VPS4 and spastin cooperate to coordinate nuclear envelope sealing and spindle disassembly PMID:26040713 GFP-CHMP4B transiently localises to telophase nuclei |
| GO:0005765 lysosomal membrane | IDA PMID:17984323 Functional multivesicular bodies are required for autophagic... | ACCEPT | Summary: Supported PN-relevant CHMP4B autophagy/endolysosomal annotation: lysosomal membrane. Reason: Human-neuron hSnf7-2/CHMP4B knockdown causes autophagosome accumulation, and ESCRT/MVB function is required for autophagic clearance; these annotations are retained as PN-relevant endolysosomal/autophagy context. Supporting Evidence: PMID:21975012 SiRNA knockdown of hSnf7-2 in human neurons leads to autophagosome accumulation PMID:21975012 hSnf7-2 and CHMP2B were more abundant than those containing CHMP2A PMID:17984323 Functional multivesicular bodies are required for autophagic clearance PMID:17984323 autophagic degradation is inhibited in cells depleted of ESCRT subunits UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0005828 kinetochore microtubule | IDA PMID:26040712 Spastin and ESCRT-III coordinate mitotic spindle disassembly... | KEEP AS NON CORE | Summary: Supported non-core CHMP4B centrosome/spindle/cell-cycle context: kinetochore microtubule. Reason: ESCRT-III/VPS4 perturbation affects centrosome and spindle maintenance and chromosome segregation, but these cell-cycle annotations are secondary to CHMP4B core ESCRT-III membrane-remodeling function. Supporting Evidence: PMID:20616062 depletion of individual ESCRT-III and VPS4 proteins also altered centrosome and spindle pole numbers PMID:20616062 ESCRT-III/VPS4 proteins function at centrosomes to help regulate their maintenance or proliferation and then at midbodies during abscission UniProt:Q9H444 promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase |
| GO:0005886 plasma membrane | IDA PMID:24878737 Structure of cellular ESCRT-III spirals and their relationsh... | KEEP AS NON CORE | Summary: True or plausible broad CHMP4B location: plasma membrane. Reason: This location is compatible with CHMP4B cycling between cytosol and recruited membrane-associated ESCRT-III assemblies, but it is too broad to represent the core function by itself. Supporting Evidence: UniProt:Q9H444 Cytoplasm. Cytosol. Late endosome membrane UniProt:Q9H444 Membrane-associated |
| GO:0006914 autophagy | IMP PMID:17984323 Functional multivesicular bodies are required for autophagic... | ACCEPT | Summary: Supported PN-relevant CHMP4B autophagy/endolysosomal annotation: autophagy. Reason: Human-neuron hSnf7-2/CHMP4B knockdown causes autophagosome accumulation, and ESCRT/MVB function is required for autophagic clearance; these annotations are retained as PN-relevant endolysosomal/autophagy context. Supporting Evidence: PMID:21975012 SiRNA knockdown of hSnf7-2 in human neurons leads to autophagosome accumulation PMID:21975012 hSnf7-2 and CHMP2B were more abundant than those containing CHMP2A PMID:17984323 Functional multivesicular bodies are required for autophagic clearance PMID:17984323 autophagic degradation is inhibited in cells depleted of ESCRT subunits UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0006997 nucleus organization | IMP PMID:20616062 Human ESCRT-III and VPS4 proteins are required for centrosom... | KEEP AS NON CORE | Summary: Supported non-core CHMP4B centrosome/spindle/cell-cycle context: nucleus organization. Reason: ESCRT-III/VPS4 perturbation affects centrosome and spindle maintenance and chromosome segregation, but these cell-cycle annotations are secondary to CHMP4B core ESCRT-III membrane-remodeling function. Supporting Evidence: PMID:20616062 depletion of individual ESCRT-III and VPS4 proteins also altered centrosome and spindle pole numbers PMID:20616062 ESCRT-III/VPS4 proteins function at centrosomes to help regulate their maintenance or proliferation and then at midbodies during abscission UniProt:Q9H444 promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase |
| GO:0007080 mitotic metaphase chromosome alignment | IMP PMID:20616062 Human ESCRT-III and VPS4 proteins are required for centrosom... | KEEP AS NON CORE | Summary: Supported non-core CHMP4B centrosome/spindle/cell-cycle context: mitotic metaphase chromosome alignment. Reason: ESCRT-III/VPS4 perturbation affects centrosome and spindle maintenance and chromosome segregation, but these cell-cycle annotations are secondary to CHMP4B core ESCRT-III membrane-remodeling function. Supporting Evidence: PMID:20616062 depletion of individual ESCRT-III and VPS4 proteins also altered centrosome and spindle pole numbers PMID:20616062 ESCRT-III/VPS4 proteins function at centrosomes to help regulate their maintenance or proliferation and then at midbodies during abscission UniProt:Q9H444 promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase |
| GO:0030496 midbody | IDA PMID:26040712 Spastin and ESCRT-III coordinate mitotic spindle disassembly... | KEEP AS NON CORE | Summary: Supported non-core CHMP4B cytokinesis/abscission context: midbody. Reason: CHMP4B has strong evidence in ESCRT-dependent cytokinetic abscission, but this is a secondary topologically related ESCRT output rather than the central endosomal/proteostasis function emphasized here. Supporting Evidence: PMID:22422861 CHMP4B was again the sole paralog required PMID:22422861 GFP-CHMP4B localized transiently to the midbody arms immediately PMID:21310966 ESCRT-III co-localized with constriction zones and was required for assembly of 17-nanometer-diameter filaments UniProt:Q9H444 functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis |
| GO:0031468 nuclear membrane reassembly | IMP PMID:26040713 ESCRT-III controls nuclear envelope reformation. | KEEP AS NON CORE | Summary: Supported non-core CHMP4B nuclear-envelope/mitotic-exit context: nuclear membrane reassembly. Reason: Nuclear envelope sealing/reassembly is a supported ESCRT-III context but is secondary to CHMP4B core ESCRT-III MVB/endosomal membrane remodeling. Supporting Evidence: UniProt:Q9H444 promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase PMID:26040712 ESCRT-III, VPS4 and spastin cooperate to coordinate nuclear envelope sealing and spindle disassembly PMID:26040713 GFP-CHMP4B transiently localises to telophase nuclei |
| GO:0032585 multivesicular body membrane | IDA PMID:16554368 The ESCRT-III subunit hVps24 is required for degradation but... | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: multivesicular body membrane. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0036258 multivesicular body assembly | NAS PMID:16505166 Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a... | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: multivesicular body assembly. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0039702 viral budding via host ESCRT complex | IDA PMID:24878737 Structure of cellular ESCRT-III spirals and their relationsh... | KEEP AS NON CORE | Summary: Supported non-core CHMP4B viral budding context: viral budding via host ESCRT complex. Reason: CHMP4B is specifically required for ESCRT-dependent HIV/viral budding, but viral budding is a pathogen-hijacked ESCRT output rather than a core host proteostasis function. Supporting Evidence: UniProt:Q9H444 Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release PMID:22422861 only depletion of CHMP4B inhibited this ESCRT-dependent process PMID:24878737 ESCRT machinery along with the AAA+ ATPase Vps4 drive membrane scission |
| GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway | IDA PMID:17984323 Functional multivesicular bodies are required for autophagic... | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0046761 viral budding from plasma membrane | IDA PMID:24878737 Structure of cellular ESCRT-III spirals and their relationsh... | KEEP AS NON CORE | Summary: Supported non-core CHMP4B viral budding context: viral budding from plasma membrane. Reason: CHMP4B is specifically required for ESCRT-dependent HIV/viral budding, but viral budding is a pathogen-hijacked ESCRT output rather than a core host proteostasis function. Supporting Evidence: UniProt:Q9H444 Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release PMID:22422861 only depletion of CHMP4B inhibited this ESCRT-dependent process PMID:24878737 ESCRT machinery along with the AAA+ ATPase Vps4 drive membrane scission |
| GO:0051469 vesicle fusion with vacuole | NAS PMID:16505166 Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a... | MODIFY | Summary: CHMP4B annotation should be replaced with a more accurate ESCRT-III term: vesicle fusion with vacuole. Reason: The evidence supports ESCRT/MVB delivery toward lysosomes rather than CHMP4B acting as a vacuole-fusion SNARE. Proposed replacements: late endosome to lysosome transport Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting |
| GO:0061763 multivesicular body-lysosome fusion | NAS PMID:16505166 Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a... | MODIFY | Summary: CHMP4B annotation should be replaced with a more accurate ESCRT-III term: multivesicular body-lysosome fusion. Reason: The evidence supports late endosome-to-lysosome/endolysosomal transport rather than CHMP4B as a direct MVB-lysosome fusion factor. Proposed replacements: late endosome to lysosome transport Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting |
| GO:0061952 midbody abscission | IMP PMID:20616062 Human ESCRT-III and VPS4 proteins are required for centrosom... | KEEP AS NON CORE | Summary: Supported non-core CHMP4B cytokinesis/abscission context: midbody abscission. Reason: CHMP4B has strong evidence in ESCRT-dependent cytokinetic abscission, but this is a secondary topologically related ESCRT output rather than the central endosomal/proteostasis function emphasized here. Supporting Evidence: PMID:22422861 CHMP4B was again the sole paralog required PMID:22422861 GFP-CHMP4B localized transiently to the midbody arms immediately PMID:21310966 ESCRT-III co-localized with constriction zones and was required for assembly of 17-nanometer-diameter filaments UniProt:Q9H444 functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis |
| GO:0071985 multivesicular body sorting pathway | IDA PMID:16554368 The ESCRT-III subunit hVps24 is required for degradation but... | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: multivesicular body sorting pathway. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0090148 membrane fission | NAS PMID:19234443 Membrane scission by the ESCRT-III complex. | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: membrane fission. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0097352 autophagosome maturation | IMP PMID:17984323 Functional multivesicular bodies are required for autophagic... | ACCEPT | Summary: Supported PN-relevant CHMP4B autophagy/endolysosomal annotation: autophagosome maturation. Reason: Human-neuron hSnf7-2/CHMP4B knockdown causes autophagosome accumulation, and ESCRT/MVB function is required for autophagic clearance; these annotations are retained as PN-relevant endolysosomal/autophagy context. Supporting Evidence: PMID:21975012 SiRNA knockdown of hSnf7-2 in human neurons leads to autophagosome accumulation PMID:21975012 hSnf7-2 and CHMP2B were more abundant than those containing CHMP2A PMID:17984323 Functional multivesicular bodies are required for autophagic clearance PMID:17984323 autophagic degradation is inhibited in cells depleted of ESCRT subunits UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:1901673 regulation of mitotic spindle assembly | IMP PMID:20616062 Human ESCRT-III and VPS4 proteins are required for centrosom... | KEEP AS NON CORE | Summary: Supported non-core CHMP4B centrosome/spindle/cell-cycle context: regulation of mitotic spindle assembly. Reason: ESCRT-III/VPS4 perturbation affects centrosome and spindle maintenance and chromosome segregation, but these cell-cycle annotations are secondary to CHMP4B core ESCRT-III membrane-remodeling function. Supporting Evidence: PMID:20616062 depletion of individual ESCRT-III and VPS4 proteins also altered centrosome and spindle pole numbers PMID:20616062 ESCRT-III/VPS4 proteins function at centrosomes to help regulate their maintenance or proliferation and then at midbodies during abscission UniProt:Q9H444 promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase |
| GO:1902774 late endosome to lysosome transport | IMP PMID:17984323 Functional multivesicular bodies are required for autophagic... | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: late endosome to lysosome transport. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:1904930 amphisome membrane | IDA PMID:17984323 Functional multivesicular bodies are required for autophagic... | ACCEPT | Summary: Supported PN-relevant CHMP4B autophagy/endolysosomal annotation: amphisome membrane. Reason: Human-neuron hSnf7-2/CHMP4B knockdown causes autophagosome accumulation, and ESCRT/MVB function is required for autophagic clearance; these annotations are retained as PN-relevant endolysosomal/autophagy context. Supporting Evidence: PMID:21975012 SiRNA knockdown of hSnf7-2 in human neurons leads to autophagosome accumulation PMID:21975012 hSnf7-2 and CHMP2B were more abundant than those containing CHMP2A PMID:17984323 Functional multivesicular bodies are required for autophagic clearance PMID:17984323 autophagic degradation is inhibited in cells depleted of ESCRT subunits UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0030496 midbody | EXP PMID:22422861 ESCRT-III governs the Aurora B-mediated abscission checkpoin... | KEEP AS NON CORE | Summary: Supported non-core CHMP4B cytokinesis/abscission context: midbody. Reason: CHMP4B has strong evidence in ESCRT-dependent cytokinetic abscission, but this is a secondary topologically related ESCRT output rather than the central endosomal/proteostasis function emphasized here. Supporting Evidence: PMID:22422861 CHMP4B was again the sole paralog required PMID:22422861 GFP-CHMP4B localized transiently to the midbody arms immediately PMID:21310966 ESCRT-III co-localized with constriction zones and was required for assembly of 17-nanometer-diameter filaments UniProt:Q9H444 functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis |
| GO:0031902 late endosome membrane | EXP PMID:15511219 Human CHMP6, a myristoylated ESCRT-III protein, interacts di... | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: late endosome membrane. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0005515 protein binding | IPI PMID:33349255 Lethal (2) giant discs (Lgd)/CC2D1 is required for the full ... | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP4B molecular-function annotation: protein binding. Reason: The cited interaction may be real, but protein binding is not informative for CHMP4B; the supported molecular function is ESCRT-III polymerization and membrane bending/scission on endosomal and related membranes. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-notes.md Generic protein binding rows were treated as over-annotated because they do not capture the ESCRT-III polymer/membrane-remodeling function. |
| GO:0050877 nervous system process | IMP PMID:21975012 ESCRT-III subunits Snf7-1 and Snf7-2 differentially regulate... | KEEP AS NON CORE | Summary: Supported but broad non-core CHMP4B neuronal context. Reason: hSnf7-2/CHMP4B is required for human neuronal survival and cargo turnover, but nervous system process is broad compared with the underlying ESCRT/MVB cargo-sorting mechanism. Supporting Evidence: PMID:21975012 hSnf7-1 and hSnf7-2 are required for the survival of human neurons PMID:21975012 hSnf7-1- or hSnf7-2-contaning ESCRT-III regulates the trafficking of different transmembrane cargos |
| GO:0071985 multivesicular body sorting pathway | IMP PMID:21975012 ESCRT-III subunits Snf7-1 and Snf7-2 differentially regulate... | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: multivesicular body sorting pathway. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0005515 protein binding | IPI PMID:34818527 The ESCRT machinery counteracts Nesprin-2G-mediated mechanic... | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP4B molecular-function annotation: protein binding. Reason: The cited interaction may be real, but protein binding is not informative for CHMP4B; the supported molecular function is ESCRT-III polymerization and membrane bending/scission on endosomal and related membranes. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-notes.md Generic protein binding rows were treated as over-annotated because they do not capture the ESCRT-III polymer/membrane-remodeling function. |
| GO:0005515 protein binding | IPI PMID:22162750 Structure of the Bro1 domain protein BROX and functional ana... | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP4B molecular-function annotation: protein binding. Reason: The cited interaction may be real, but protein binding is not informative for CHMP4B; the supported molecular function is ESCRT-III polymerization and membrane bending/scission on endosomal and related membranes. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-notes.md Generic protein binding rows were treated as over-annotated because they do not capture the ESCRT-III polymer/membrane-remodeling function. |
| GO:0046761 viral budding from plasma membrane | IMP PMID:14505570 The protein network of HIV budding. | KEEP AS NON CORE | Summary: Supported non-core CHMP4B viral budding context: viral budding from plasma membrane. Reason: CHMP4B is specifically required for ESCRT-dependent HIV/viral budding, but viral budding is a pathogen-hijacked ESCRT output rather than a core host proteostasis function. Supporting Evidence: UniProt:Q9H444 Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release PMID:22422861 only depletion of CHMP4B inhibited this ESCRT-dependent process PMID:24878737 ESCRT machinery along with the AAA+ ATPase Vps4 drive membrane scission |
| GO:0000815 ESCRT III complex | IDA PMID:18209100 Plasma membrane deformation by circular arrays of ESCRT-III ... | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: ESCRT III complex. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0042802 identical protein binding | IPI PMID:18209100 Plasma membrane deformation by circular arrays of ESCRT-III ... | MODIFY | Summary: CHMP4B annotation should be replaced with a more accurate ESCRT-III term: identical protein binding. Reason: Identical protein binding is less informative than CHMP4B/Snf7-family polymerization and membrane bending. Proposed replacements: protein polymerization membrane bending activity Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting |
| GO:0051258 protein polymerization | IDA PMID:18209100 Plasma membrane deformation by circular arrays of ESCRT-III ... | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: protein polymerization. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0045296 cadherin binding | HDA PMID:25468996 E-cadherin interactome complexity and robustness resolved by... | MARK AS OVER ANNOTATED | Summary: Broad high-throughput/proximity annotation is not a core CHMP4B function: cadherin binding. Reason: The E-cadherin proteomics evidence is broad proximity/localization evidence and does not establish cadherin binding or generic vesicle localization as an informative CHMP4B function. Supporting Evidence: PMID:25468996 proximity biotinylation and quantitative proteomics to identify 561 proteins in the vicinity file:human/CHMP4B/CHMP4B-notes.md The E-cadherin proximity proteomics evidence is broad high-throughput context, not a core CHMP4B molecular function. |
| GO:0005515 protein binding | IPI PMID:18641129 Differential requirements for Alix and ESCRT-III in cytokine... | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP4B molecular-function annotation: protein binding. Reason: The cited interaction may be real, but protein binding is not informative for CHMP4B; the supported molecular function is ESCRT-III polymerization and membrane bending/scission on endosomal and related membranes. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-notes.md Generic protein binding rows were treated as over-annotated because they do not capture the ESCRT-III polymer/membrane-remodeling function. |
| GO:0016236 macroautophagy | TAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a... | ACCEPT | Summary: Supported PN-relevant CHMP4B autophagy/endolysosomal annotation: macroautophagy. Reason: Human-neuron hSnf7-2/CHMP4B knockdown causes autophagosome accumulation, and ESCRT/MVB function is required for autophagic clearance; these annotations are retained as PN-relevant endolysosomal/autophagy context. Supporting Evidence: PMID:21975012 SiRNA knockdown of hSnf7-2 in human neurons leads to autophagosome accumulation PMID:21975012 hSnf7-2 and CHMP2B were more abundant than those containing CHMP2A PMID:17984323 Functional multivesicular bodies are required for autophagic clearance PMID:17984323 autophagic degradation is inhibited in cells depleted of ESCRT subunits UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0031982 vesicle | IDA PMID:25468996 E-cadherin interactome complexity and robustness resolved by... | MARK AS OVER ANNOTATED | Summary: Broad high-throughput/proximity annotation is not a core CHMP4B function: vesicle. Reason: The E-cadherin proteomics evidence is broad proximity/localization evidence and does not establish cadherin binding or generic vesicle localization as an informative CHMP4B function. Supporting Evidence: PMID:25468996 proximity biotinylation and quantitative proteomics to identify 561 proteins in the vicinity file:human/CHMP4B/CHMP4B-notes.md The E-cadherin proximity proteomics evidence is broad high-throughput context, not a core CHMP4B molecular function. |
| GO:0000281 mitotic cytokinesis | IMP PMID:21310966 Cortical constriction during abscission involves helices of ... | MODIFY | Summary: CHMP4B annotation should be replaced with a more accurate ESCRT-III term: mitotic cytokinesis. Reason: CHMP4B is specifically implicated in ESCRT-dependent midbody abscission rather than all of mitotic cytokinesis. Proposed replacements: midbody abscission Supporting Evidence: PMID:22422861 CHMP4B was again the sole paralog required PMID:22422861 GFP-CHMP4B localized transiently to the midbody arms immediately PMID:21310966 ESCRT-III co-localized with constriction zones and was required for assembly of 17-nanometer-diameter filaments UniProt:Q9H444 functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis |
| GO:0000815 ESCRT III complex | IDA PMID:21310966 Cortical constriction during abscission involves helices of ... | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: ESCRT III complex. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0000815 ESCRT III complex | IDA PMID:26040712 Spastin and ESCRT-III coordinate mitotic spindle disassembly... | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: ESCRT III complex. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0005635 nuclear envelope | IDA PMID:26040712 Spastin and ESCRT-III coordinate mitotic spindle disassembly... | KEEP AS NON CORE | Summary: Supported non-core CHMP4B nuclear-envelope/mitotic-exit context: nuclear envelope. Reason: Nuclear envelope sealing/reassembly is a supported ESCRT-III context but is secondary to CHMP4B core ESCRT-III MVB/endosomal membrane remodeling. Supporting Evidence: UniProt:Q9H444 promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase PMID:26040712 ESCRT-III, VPS4 and spastin cooperate to coordinate nuclear envelope sealing and spindle disassembly PMID:26040713 GFP-CHMP4B transiently localises to telophase nuclei |
| GO:0010458 exit from mitosis | IMP PMID:26040712 Spastin and ESCRT-III coordinate mitotic spindle disassembly... | KEEP AS NON CORE | Summary: Supported non-core CHMP4B nuclear-envelope/mitotic-exit context: exit from mitosis. Reason: Nuclear envelope sealing/reassembly is a supported ESCRT-III context but is secondary to CHMP4B core ESCRT-III MVB/endosomal membrane remodeling. Supporting Evidence: UniProt:Q9H444 promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase PMID:26040712 ESCRT-III, VPS4 and spastin cooperate to coordinate nuclear envelope sealing and spindle disassembly PMID:26040713 GFP-CHMP4B transiently localises to telophase nuclei |
| GO:0030496 midbody | IDA PMID:21310966 Cortical constriction during abscission involves helices of ... | KEEP AS NON CORE | Summary: Supported non-core CHMP4B cytokinesis/abscission context: midbody. Reason: CHMP4B has strong evidence in ESCRT-dependent cytokinetic abscission, but this is a secondary topologically related ESCRT output rather than the central endosomal/proteostasis function emphasized here. Supporting Evidence: PMID:22422861 CHMP4B was again the sole paralog required PMID:22422861 GFP-CHMP4B localized transiently to the midbody arms immediately PMID:21310966 ESCRT-III co-localized with constriction zones and was required for assembly of 17-nanometer-diameter filaments UniProt:Q9H444 functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis |
| GO:0031468 nuclear membrane reassembly | IMP PMID:26040712 Spastin and ESCRT-III coordinate mitotic spindle disassembly... | KEEP AS NON CORE | Summary: Supported non-core CHMP4B nuclear-envelope/mitotic-exit context: nuclear membrane reassembly. Reason: Nuclear envelope sealing/reassembly is a supported ESCRT-III context but is secondary to CHMP4B core ESCRT-III MVB/endosomal membrane remodeling. Supporting Evidence: UniProt:Q9H444 promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase PMID:26040712 ESCRT-III, VPS4 and spastin cooperate to coordinate nuclear envelope sealing and spindle disassembly PMID:26040713 GFP-CHMP4B transiently localises to telophase nuclei |
| GO:0090148 membrane fission | IMP PMID:21310966 Cortical constriction during abscission involves helices of ... | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: membrane fission. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0006914 autophagy | IMP PMID:24095276 Syntaxin 13, a genetic modifier of mutant CHMP2B in frontote... | ACCEPT | Summary: Supported PN-relevant CHMP4B autophagy/endolysosomal annotation: autophagy. Reason: Human-neuron hSnf7-2/CHMP4B knockdown causes autophagosome accumulation, and ESCRT/MVB function is required for autophagic clearance; these annotations are retained as PN-relevant endolysosomal/autophagy context. Supporting Evidence: PMID:21975012 SiRNA knockdown of hSnf7-2 in human neurons leads to autophagosome accumulation PMID:21975012 hSnf7-2 and CHMP2B were more abundant than those containing CHMP2A PMID:17984323 Functional multivesicular bodies are required for autophagic clearance PMID:17984323 autophagic degradation is inhibited in cells depleted of ESCRT subunits UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0000815 ESCRT III complex | TAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a... | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: ESCRT III complex. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0036258 multivesicular body assembly | TAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a... | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: multivesicular body assembly. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0039702 viral budding via host ESCRT complex | TAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a... | KEEP AS NON CORE | Summary: Supported non-core CHMP4B viral budding context: viral budding via host ESCRT complex. Reason: CHMP4B is specifically required for ESCRT-dependent HIV/viral budding, but viral budding is a pathogen-hijacked ESCRT output rather than a core host proteostasis function. Supporting Evidence: UniProt:Q9H444 Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release PMID:22422861 only depletion of CHMP4B inhibited this ESCRT-dependent process PMID:24878737 ESCRT machinery along with the AAA+ ATPase Vps4 drive membrane scission |
| GO:0006914 autophagy | TAS PMID:21118109 The role of ESCRT proteins in fusion events involving lysoso... | ACCEPT | Summary: Supported PN-relevant CHMP4B autophagy/endolysosomal annotation: autophagy. Reason: Human-neuron hSnf7-2/CHMP4B knockdown causes autophagosome accumulation, and ESCRT/MVB function is required for autophagic clearance; these annotations are retained as PN-relevant endolysosomal/autophagy context. Supporting Evidence: PMID:21975012 SiRNA knockdown of hSnf7-2 in human neurons leads to autophagosome accumulation PMID:21975012 hSnf7-2 and CHMP2B were more abundant than those containing CHMP2A PMID:17984323 Functional multivesicular bodies are required for autophagic clearance PMID:17984323 autophagic degradation is inhibited in cells depleted of ESCRT subunits UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0000815 ESCRT III complex | IDA PMID:24878737 Structure of cellular ESCRT-III spirals and their relationsh... | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: ESCRT III complex. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0030117 membrane coat | IDA PMID:24878737 Structure of cellular ESCRT-III spirals and their relationsh... | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: membrane coat. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0005737 cytoplasm | IDA PMID:17701905 CHMP4B, a novel gene for autosomal dominant cataracts linked... | KEEP AS NON CORE | Summary: True or plausible broad CHMP4B location: cytoplasm. Reason: This location is compatible with CHMP4B cycling between cytosol and recruited membrane-associated ESCRT-III assemblies, but it is too broad to represent the core function by itself. Supporting Evidence: UniProt:Q9H444 Cytoplasm. Cytosol. Late endosome membrane UniProt:Q9H444 Membrane-associated |
| GO:0005768 endosome | IDA PMID:17701905 CHMP4B, a novel gene for autosomal dominant cataracts linked... | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: endosome. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0036438 maintenance of lens transparency | IMP PMID:17701905 CHMP4B, a novel gene for autosomal dominant cataracts linked... | KEEP AS NON CORE | Summary: Supported non-core CHMP4B lens-transparency disease/physiology context. Reason: CHMP4B variants cause dominant cataracts and support a lens-transparency phenotype, but this phenotype does not redefine the core ESCRT-III molecular function. Supporting Evidence: PMID:17701905 plays a vital role in the maintenance of lens transparency PMID:17701905 key component of the endosome sorting complex required |
| GO:0046755 viral budding | IMP PMID:17701905 CHMP4B, a novel gene for autosomal dominant cataracts linked... | MODIFY | Summary: CHMP4B annotation should be replaced with a more accurate ESCRT-III term: viral budding. Reason: The viral-budding evidence is specifically host ESCRT-mediated budding, not generic viral budding. Proposed replacements: viral budding via host ESCRT complex Supporting Evidence: UniProt:Q9H444 Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release PMID:22422861 only depletion of CHMP4B inhibited this ESCRT-dependent process PMID:24878737 ESCRT machinery along with the AAA+ ATPase Vps4 drive membrane scission |
| GO:0010824 regulation of centrosome duplication | IMP PMID:20616062 Human ESCRT-III and VPS4 proteins are required for centrosom... | KEEP AS NON CORE | Summary: Supported non-core CHMP4B centrosome/spindle/cell-cycle context: regulation of centrosome duplication. Reason: ESCRT-III/VPS4 perturbation affects centrosome and spindle maintenance and chromosome segregation, but these cell-cycle annotations are secondary to CHMP4B core ESCRT-III membrane-remodeling function. Supporting Evidence: PMID:20616062 depletion of individual ESCRT-III and VPS4 proteins also altered centrosome and spindle pole numbers PMID:20616062 ESCRT-III/VPS4 proteins function at centrosomes to help regulate their maintenance or proliferation and then at midbodies during abscission UniProt:Q9H444 promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase |
| GO:0090611 obsolete ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway | IMP PMID:22547407 ALIX binds a YPX(3)L motif of the GPCR PAR1 and mediates ubi... | MODIFY | Summary: CHMP4B annotation should be replaced with a more accurate ESCRT-III term: obsolete ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway. Reason: This obsolete ubiquitin-independent catabolic process term should be replaced by the non-obsolete MVB sorting pathway term. Proposed replacements: multivesicular body sorting pathway Supporting Evidence: PMID:22547407 PAR1 sorted to ILVs of MVBs through an ESCRT-III-dependent pathway independent of ubiquitination PMID:22547407 ALIX binds to a YPX3L motif of PAR1 and recruits ESCRT-III to mediate MVB/lysosomal sorting |
| GO:0039702 viral budding via host ESCRT complex | IGI PMID:24107264 ESCRT requirements for EIAV budding. | KEEP AS NON CORE | Summary: Supported non-core CHMP4B viral budding context: viral budding via host ESCRT complex. Reason: CHMP4B is specifically required for ESCRT-dependent HIV/viral budding, but viral budding is a pathogen-hijacked ESCRT output rather than a core host proteostasis function. Supporting Evidence: UniProt:Q9H444 Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release PMID:22422861 only depletion of CHMP4B inhibited this ESCRT-dependent process PMID:24878737 ESCRT machinery along with the AAA+ ATPase Vps4 drive membrane scission |
| GO:0070062 extracellular exosome | HDA PMID:23533145 In-depth proteomic analyses of exosomes isolated from expres... | KEEP AS NON CORE | Summary: Plausible but broad non-core CHMP4B extracellular-exosome context. Reason: CHMP4B/ESCRT biology supports exosome release, but high-throughput extracellular exosome localization is broad and secondary to the core ESCRT-III MVB membrane-remodeling function. Supporting Evidence: UniProt:Q9H444 CHMP4A/B/C are required for the exosomal release of SDCBP, CD63 and syndecan PMID:18209100 MVBs also fuse with the plasma membrane to secrete their ILVs as entities called exosomes |
| GO:0005515 protein binding | IPI PMID:14519844 Divergent retroviral late-budding domains recruit vacuolar p... | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP4B molecular-function annotation: protein binding. Reason: The cited interaction may be real, but protein binding is not informative for CHMP4B; the supported molecular function is ESCRT-III polymerization and membrane bending/scission on endosomal and related membranes. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-notes.md Generic protein binding rows were treated as over-annotated because they do not capture the ESCRT-III polymer/membrane-remodeling function. |
| GO:0042803 protein homodimerization activity | IPI PMID:14519844 Divergent retroviral late-budding domains recruit vacuolar p... | MODIFY | Summary: CHMP4B annotation should be replaced with a more accurate ESCRT-III term: protein homodimerization activity. Reason: Homodimerization is less informative than CHMP4B/Snf7-family polymerization and membrane bending. Proposed replacements: protein polymerization membrane bending activity Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting |
| GO:0005515 protein binding | IPI PMID:14505570 The protein network of HIV budding. | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP4B molecular-function annotation: protein binding. Reason: The cited interaction may be real, but protein binding is not informative for CHMP4B; the supported molecular function is ESCRT-III polymerization and membrane bending/scission on endosomal and related membranes. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-notes.md Generic protein binding rows were treated as over-annotated because they do not capture the ESCRT-III polymer/membrane-remodeling function. |
| GO:0000815 ESCRT III complex | IDA PMID:21975012 ESCRT-III subunits Snf7-1 and Snf7-2 differentially regulate... | ACCEPT | Summary: Supported core CHMP4B ESCRT-III/MVB membrane-remodeling annotation: ESCRT III complex. Reason: CHMP4B is a Snf7-family ESCRT-III core subunit whose polymerization on endosomal/MVB membranes supports cargo sorting, membrane bending, and reverse-topology membrane fission. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks PMID:12860994 CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting PMID:15511219 CHMP6 acts as an acceptor for ESCRT-II on endosomal membranes and regulates cargo sorting file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
| GO:0005515 protein binding | IPI PMID:21975012 ESCRT-III subunits Snf7-1 and Snf7-2 differentially regulate... | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP4B molecular-function annotation: protein binding. Reason: The cited interaction may be real, but protein binding is not informative for CHMP4B; the supported molecular function is ESCRT-III polymerization and membrane bending/scission on endosomal and related membranes. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-notes.md Generic protein binding rows were treated as over-annotated because they do not capture the ESCRT-III polymer/membrane-remodeling function. |
| GO:0005634 nucleus | IDA PMID:21975012 ESCRT-III subunits Snf7-1 and Snf7-2 differentially regulate... | KEEP AS NON CORE | Summary: True or plausible broad CHMP4B location: nucleus. Reason: This location is compatible with CHMP4B cycling between cytosol and recruited membrane-associated ESCRT-III assemblies, but it is too broad to represent the core function by itself. Supporting Evidence: UniProt:Q9H444 Cytoplasm. Cytosol. Late endosome membrane UniProt:Q9H444 Membrane-associated |
| GO:0005737 cytoplasm | IDA PMID:21975012 ESCRT-III subunits Snf7-1 and Snf7-2 differentially regulate... | KEEP AS NON CORE | Summary: True or plausible broad CHMP4B location: cytoplasm. Reason: This location is compatible with CHMP4B cycling between cytosol and recruited membrane-associated ESCRT-III assemblies, but it is too broad to represent the core function by itself. Supporting Evidence: UniProt:Q9H444 Cytoplasm. Cytosol. Late endosome membrane UniProt:Q9H444 Membrane-associated |
| GO:0006620 post-translational protein targeting to endoplasmic reticulum membrane | IMP PMID:21975012 ESCRT-III subunits Snf7-1 and Snf7-2 differentially regulate... | REMOVE | Summary: Unsupported CHMP4B annotation: post-translational protein targeting to ER membrane. Reason: PMID:21975012 supports hSnf7-2/CHMP4B in neuronal endosomal cargo turnover and autophagosome accumulation, but does not support a role in post-translational targeting to the ER membrane. Supporting Evidence: PMID:21975012 hSnf7-2-containing ESCRT-III differentially regulates the turnover of distinct transmembrane cargos in human neurons PMID:21975012 SiRNA knockdown of hSnf7-2 in human neurons leads to autophagosome accumulation file:human/CHMP4B/CHMP4B-notes.md PMID:21975012 supports neuronal cargo turnover and autophagosome accumulation, not post-translational targeting to the ER membrane. |
| GO:0009898 cytoplasmic side of plasma membrane | IDA PMID:18209100 Plasma membrane deformation by circular arrays of ESCRT-III ... | KEEP AS NON CORE | Summary: True or plausible broad CHMP4B location: cytoplasmic side of plasma membrane. Reason: This location is compatible with CHMP4B cycling between cytosol and recruited membrane-associated ESCRT-III assemblies, but it is too broad to represent the core function by itself. Supporting Evidence: UniProt:Q9H444 Cytoplasm. Cytosol. Late endosome membrane UniProt:Q9H444 Membrane-associated |
| GO:0070062 extracellular exosome | HDA PMID:19056867 Large-scale proteomics and phosphoproteomics of urinary exos... | KEEP AS NON CORE | Summary: Plausible but broad non-core CHMP4B extracellular-exosome context. Reason: CHMP4B/ESCRT biology supports exosome release, but high-throughput extracellular exosome localization is broad and secondary to the core ESCRT-III MVB membrane-remodeling function. Supporting Evidence: UniProt:Q9H444 CHMP4A/B/C are required for the exosomal release of SDCBP, CD63 and syndecan PMID:18209100 MVBs also fuse with the plasma membrane to secrete their ILVs as entities called exosomes |
| GO:0005515 protein binding | IPI PMID:12860994 The ALG-2-interacting protein Alix associates with CHMP4b, a... | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP4B molecular-function annotation: protein binding. Reason: The cited interaction may be real, but protein binding is not informative for CHMP4B; the supported molecular function is ESCRT-III polymerization and membrane bending/scission on endosomal and related membranes. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-notes.md Generic protein binding rows were treated as over-annotated because they do not capture the ESCRT-III polymer/membrane-remodeling function. |
| GO:0070062 extracellular exosome | HDA PMID:20458337 MHC class II-associated proteins in B-cell exosomes and pote... | KEEP AS NON CORE | Summary: Plausible but broad non-core CHMP4B extracellular-exosome context. Reason: CHMP4B/ESCRT biology supports exosome release, but high-throughput extracellular exosome localization is broad and secondary to the core ESCRT-III MVB membrane-remodeling function. Supporting Evidence: UniProt:Q9H444 CHMP4A/B/C are required for the exosomal release of SDCBP, CD63 and syndecan PMID:18209100 MVBs also fuse with the plasma membrane to secrete their ILVs as entities called exosomes |
| GO:0005515 protein binding | IPI PMID:21543490 Mechanism of inhibition of retrovirus release from cells by ... | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP4B molecular-function annotation: protein binding. Reason: The cited interaction may be real, but protein binding is not informative for CHMP4B; the supported molecular function is ESCRT-III polymerization and membrane bending/scission on endosomal and related membranes. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-notes.md Generic protein binding rows were treated as over-annotated because they do not capture the ESCRT-III polymer/membrane-remodeling function. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-3159232 | KEEP AS NON CORE | Summary: True or plausible broad CHMP4B location: cytosol. Reason: This location is compatible with CHMP4B cycling between cytosol and recruited membrane-associated ESCRT-III assemblies, but it is too broad to represent the core function by itself. Supporting Evidence: UniProt:Q9H444 Cytoplasm. Cytosol. Late endosome membrane UniProt:Q9H444 Membrane-associated |
| GO:0005829 cytosol | TAS Reactome:R-HSA-917693 | KEEP AS NON CORE | Summary: True or plausible broad CHMP4B location: cytosol. Reason: This location is compatible with CHMP4B cycling between cytosol and recruited membrane-associated ESCRT-III assemblies, but it is too broad to represent the core function by itself. Supporting Evidence: UniProt:Q9H444 Cytoplasm. Cytosol. Late endosome membrane UniProt:Q9H444 Membrane-associated |
| GO:0005829 cytosol | TAS Reactome:R-HSA-917700 | KEEP AS NON CORE | Summary: True or plausible broad CHMP4B location: cytosol. Reason: This location is compatible with CHMP4B cycling between cytosol and recruited membrane-associated ESCRT-III assemblies, but it is too broad to represent the core function by itself. Supporting Evidence: UniProt:Q9H444 Cytoplasm. Cytosol. Late endosome membrane UniProt:Q9H444 Membrane-associated |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9668389 | KEEP AS NON CORE | Summary: True or plausible broad CHMP4B location: cytosol. Reason: This location is compatible with CHMP4B cycling between cytosol and recruited membrane-associated ESCRT-III assemblies, but it is too broad to represent the core function by itself. Supporting Evidence: UniProt:Q9H444 Cytoplasm. Cytosol. Late endosome membrane UniProt:Q9H444 Membrane-associated |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9668395 | KEEP AS NON CORE | Summary: True or plausible broad CHMP4B location: cytosol. Reason: This location is compatible with CHMP4B cycling between cytosol and recruited membrane-associated ESCRT-III assemblies, but it is too broad to represent the core function by itself. Supporting Evidence: UniProt:Q9H444 Cytoplasm. Cytosol. Late endosome membrane UniProt:Q9H444 Membrane-associated |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9668398 | KEEP AS NON CORE | Summary: True or plausible broad CHMP4B location: cytosol. Reason: This location is compatible with CHMP4B cycling between cytosol and recruited membrane-associated ESCRT-III assemblies, but it is too broad to represent the core function by itself. Supporting Evidence: UniProt:Q9H444 Cytoplasm. Cytosol. Late endosome membrane UniProt:Q9H444 Membrane-associated |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9668405 | KEEP AS NON CORE | Summary: True or plausible broad CHMP4B location: cytosol. Reason: This location is compatible with CHMP4B cycling between cytosol and recruited membrane-associated ESCRT-III assemblies, but it is too broad to represent the core function by itself. Supporting Evidence: UniProt:Q9H444 Cytoplasm. Cytosol. Late endosome membrane UniProt:Q9H444 Membrane-associated |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9668415 | KEEP AS NON CORE | Summary: True or plausible broad CHMP4B location: cytosol. Reason: This location is compatible with CHMP4B cycling between cytosol and recruited membrane-associated ESCRT-III assemblies, but it is too broad to represent the core function by itself. Supporting Evidence: UniProt:Q9H444 Cytoplasm. Cytosol. Late endosome membrane UniProt:Q9H444 Membrane-associated |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9668419 | KEEP AS NON CORE | Summary: True or plausible broad CHMP4B location: cytosol. Reason: This location is compatible with CHMP4B cycling between cytosol and recruited membrane-associated ESCRT-III assemblies, but it is too broad to represent the core function by itself. Supporting Evidence: UniProt:Q9H444 Cytoplasm. Cytosol. Late endosome membrane UniProt:Q9H444 Membrane-associated |
| GO:0030496 midbody | IDA PMID:22724069 The chromosomal passenger complex controls the function of e... | KEEP AS NON CORE | Summary: Supported non-core CHMP4B cytokinesis/abscission context: midbody. Reason: CHMP4B has strong evidence in ESCRT-dependent cytokinetic abscission, but this is a secondary topologically related ESCRT output rather than the central endosomal/proteostasis function emphasized here. Supporting Evidence: PMID:22422861 CHMP4B was again the sole paralog required PMID:22422861 GFP-CHMP4B localized transiently to the midbody arms immediately PMID:21310966 ESCRT-III co-localized with constriction zones and was required for assembly of 17-nanometer-diameter filaments UniProt:Q9H444 functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis |
| GO:0005515 protein binding | IPI PMID:20208530 PtdIns(3)P controls cytokinesis through KIF13A-mediated recr... | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP4B molecular-function annotation: protein binding. Reason: The cited interaction may be real, but protein binding is not informative for CHMP4B; the supported molecular function is ESCRT-III polymerization and membrane bending/scission on endosomal and related membranes. Supporting Evidence: UniProt:Q9H444 Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) UniProt:Q9H444 involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs file:human/CHMP4B/CHMP4B-notes.md Generic protein binding rows were treated as over-annotated because they do not capture the ESCRT-III polymer/membrane-remodeling function. |
| GO:0180020 membrane bending activity | IDA PMID:18209100 Plasma membrane deformation by circular arrays of ESCRT-III ... | NEW | Summary: New CHMP4B molecular-function annotation supported by direct hSnf7-2 filament/membrane curvature evidence. Reason: PMID:18209100 directly includes hSnf7-2/CHMP4B and shows that CHMP4-containing ESCRT-III filaments promote or stabilize negative membrane curvature and outward budding, making membrane bending activity more informative than generic binding terms. Supporting Evidence: UniProt:Q9H444 believed to mediate the necessary vesicle extrusion and/or membrane fission activities PMID:18209100 hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B) PMID:18209100 form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding PMID:18209100 assemble into regular approximately 5-nm filaments that curve and self-associate PMID:19234443 ESCRT-III has the instrinsic ability to drive the scission of membrane necks file:human/CHMP4B/CHMP4B-deep-research-falcon.md polymerizes on membranes as part of **ESCRT-III** to drive **reverse-topology membrane constriction and scission** |
Loading supporting content…
Download this section (compressed HTML)Q: Which CHMP4 paralog is the dominant Snf7-family subunit during human phagophore closure and autophagosome maturation in endogenous cells?
Q: Should GO annotations distinguish CHMP4B direct membrane-bending/polymer function from broader ESCRT output processes such as viral budding, plasma membrane repair, nuclear envelope sealing, and cytokinetic abscission?
Q: How do CHMP4B-CHMP2B-enriched ESCRT-III assemblies differ from CHMP4A-CHMP2A assemblies in neuronal endosomal cargo turnover and proteostasis?
Experiment: Use endogenous CHMP4B tagging plus acute depletion/rescue during starvation-induced autophagy and selective cargo turnover assays to image recruitment to phagophores, autophagosomes, amphisomes, and endosomes.
Hypothesis: If CHMP4B directly participates in autophagosome maturation or closure, it should be transiently recruited to autophagy membranes and rescue should require its polymerization/membrane-binding region.
Experiment: Compare wild-type CHMP4B with ALIX-binding, CHMP2B-interaction, and C-terminal autoinhibition mutants in MVB cargo sorting, HIV budding, cytokinetic abscission, and neuronal receptor turnover assays.
Hypothesis: CHMP4B uses the same Snf7-family filament core across ESCRT outputs, but partner and recruitment requirements differ by membrane context.
Experiment: Biochemically reconstitute CHMP4B-containing human ESCRT-III polymers with CHMP2B, CHMP3, CHMP6, and VPS4 and test membrane bending/scission on defined liposomes.
Hypothesis: CHMP4B contributes Snf7-family filament curvature that can drive membrane bending and support reverse-topology scission in a minimal human ESCRT-III module.
Loading supporting content…
Download this section (compressed HTML)Loading supporting content…
Download this section (compressed HTML)Loading supporting content…
Download this section (compressed HTML)Loading supporting content…
Download this section (compressed HTML)