CHMP7

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0005635 nuclear envelope	IBA GO_REF:0000033	ACCEPT	Summary: Supported core CHMP7 nuclear-envelope function: nuclear envelope. Reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for nuclear membrane closure/sealing. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery PMID:28242692 CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope UniProt:Q8WUX9 required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase file:human/CHMP7/CHMP7-notes.md the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure
GO:0031468 nuclear membrane reassembly	IBA GO_REF:0000033	ACCEPT	Summary: Supported core CHMP7 nuclear-envelope function: nuclear membrane reassembly. Reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for nuclear membrane closure/sealing. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery PMID:28242692 CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope UniProt:Q8WUX9 required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase file:human/CHMP7/CHMP7-notes.md the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure
GO:0000815 ESCRT III complex	IBA GO_REF:0000033	ACCEPT	Summary: Supported CHMP7 ESCRT-III-associated complex annotation: ESCRT III complex. Reason: CHMP7 is an ESCRT-III-like factor that recruits and associates with downstream ESCRT-III components, especially at the reforming nuclear envelope. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway file:human/CHMP7/CHMP7-notes.md CHMP7 is an ESCRT-III-like / ESCRT-II-III hybrid protein with two literature-supported contexts
GO:0009898 cytoplasmic side of plasma membrane	IBA GO_REF:0000033	MARK AS OVER ANNOTATED	Summary: Plasma-membrane location/context is over-extended for CHMP7: cytoplasmic side of plasma membrane. Reason: CHMP7-specific evidence localizes its core recruitment function to the reforming nuclear envelope; plasma membrane context comes from broader ESCRT/viral-budding pathway inference. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Treat viral budding annotations as over-annotated for CHMP7. file:human/CHMP7/CHMP7-notes.md the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure
GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway	IBA GO_REF:0000033	MODIFY	Summary: The endosomal-sorting evidence is real, but late endosome to vacuole transport via multivesicular body sorting pathway should be expressed with human MVB/endolysosomal terms. Reason: CHMP7 has an older supported endosomal-sorting phenotype, but vacuole wording is less appropriate for human curation than MVB sorting / late endosome-to-lysosome transport. Proposed replacements: multivesicular body sorting pathway late endosome to lysosome transport Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment.
GO:0000776 kinetochore	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: kinetochore. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0001778 plasma membrane repair	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: plasma membrane repair. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0005635 nuclear envelope	IEA GO_REF:0000044	ACCEPT	Summary: Supported core CHMP7 nuclear-envelope function: nuclear envelope. Reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for nuclear membrane closure/sealing. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery PMID:28242692 CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope UniProt:Q8WUX9 required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase file:human/CHMP7/CHMP7-notes.md the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure
GO:0005643 nuclear pore	IEA GO_REF:0000117	MODIFY	Summary: The localization essence is nuclear-envelope recruitment, not stable membership in nuclear pore. Reason: CHMP7 localizes to reforming nuclear-envelope/chromatin-disk sites; nuclear envelope is the more accurate cellular-component term. Proposed replacements: nuclear envelope Supporting Evidence: PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope file:human/CHMP7/CHMP7-notes.md exit from mitosis, nuclear pore, chromatin location, and spindle-assembly wording should be interpreted through the specific CHMP7 role in nuclear membrane reassembly and nuclear-envelope sealing
GO:0005737 cytoplasm	IEA GO_REF:0000044	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: cytoplasm. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0005765 lysosomal membrane	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: lysosomal membrane. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0005828 kinetochore microtubule	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: kinetochore microtubule. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0007034 vacuolar transport	IEA GO_REF:0000002	MODIFY	Summary: The endosomal-sorting evidence is real, but vacuolar transport should be expressed with human MVB/endolysosomal terms. Reason: CHMP7 has an older supported endosomal-sorting phenotype, but vacuole wording is less appropriate for human curation than MVB sorting / late endosome-to-lysosome transport. Proposed replacements: multivesicular body sorting pathway late endosome to lysosome transport Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment.
GO:0007080 mitotic metaphase chromosome alignment	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: mitotic metaphase chromosome alignment. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0030496 midbody	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: midbody. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0031468 nuclear membrane reassembly	IEA GO_REF:0000117	ACCEPT	Summary: Supported core CHMP7 nuclear-envelope function: nuclear membrane reassembly. Reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for nuclear membrane closure/sealing. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery PMID:28242692 CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope UniProt:Q8WUX9 required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase file:human/CHMP7/CHMP7-notes.md the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure
GO:0032585 multivesicular body membrane	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: Supported but secondary CHMP7 endosomal/MVB ESCRT context: multivesicular body membrane. Reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment.
GO:0039702 viral budding via host ESCRT complex	IEA GO_REF:0000117	MARK AS OVER ANNOTATED	Summary: Over-annotated CHMP7 viral-budding term: viral budding via host ESCRT complex. Reason: The CHMP7 viral evidence is a dominant-negative overexpression effect on virus-like particle release, not an endogenous core CHMP7 viral-budding function. Supporting Evidence: PMID:16856878 dominant-negative effect of overexpressed GFP-CHMP7 file:human/CHMP7/CHMP7-notes.md The direct evidence is dominant-negative overexpression affecting virus-like particle release, not a demonstrated endogenous core viral-budding function.
GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: Supported but secondary CHMP7 endosomal/MVB ESCRT context: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway. Reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment.
GO:0046761 viral budding from plasma membrane	IEA GO_REF:0000117	MARK AS OVER ANNOTATED	Summary: Over-annotated CHMP7 viral-budding term: viral budding from plasma membrane. Reason: The CHMP7 viral evidence is a dominant-negative overexpression effect on virus-like particle release, not an endogenous core CHMP7 viral-budding function. Supporting Evidence: PMID:16856878 dominant-negative effect of overexpressed GFP-CHMP7 file:human/CHMP7/CHMP7-notes.md The direct evidence is dominant-negative overexpression affecting virus-like particle release, not a demonstrated endogenous core viral-budding function.
GO:0061952 midbody abscission	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: midbody abscission. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0071985 multivesicular body sorting pathway	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: Supported but secondary CHMP7 endosomal/MVB ESCRT context: multivesicular body sorting pathway. Reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment.
GO:0097352 autophagosome maturation	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: autophagosome maturation. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:1901673 regulation of mitotic spindle assembly	IEA GO_REF:0000117	MODIFY	Summary: The cell-cycle/spindle wording is too broad for CHMP7: regulation of mitotic spindle assembly. Reason: CHMP7 contributes specifically to nuclear membrane reassembly and NE sealing during mitotic exit rather than to broad mitotic progression or spindle assembly. Proposed replacements: nuclear membrane reassembly Supporting Evidence: PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope file:human/CHMP7/CHMP7-notes.md exit from mitosis, nuclear pore, chromatin location, and spindle-assembly wording should be interpreted through the specific CHMP7 role in nuclear membrane reassembly and nuclear-envelope sealing
GO:1902774 late endosome to lysosome transport	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: Supported but secondary CHMP7 endosomal/MVB ESCRT context: late endosome to lysosome transport. Reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment.
GO:1904930 amphisome membrane	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: amphisome membrane. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0005515 protein binding	IPI PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CH...	MARK AS OVER ANNOTATED	Summary: Generic protein binding is over-annotated for CHMP7: protein binding. Reason: The meaningful interactions are CHMP7-LEMD2 and CHMP7-CHMP4B; GO:0005515 is too generic to represent CHMP7 function. Supporting Evidence: PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b PMID:28242692 CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro file:human/CHMP7/CHMP7-notes.md The meaningful molecular interactions are CHMP7-LEMD2 and CHMP7-CHMP4B, not undifferentiated protein binding.
GO:0005515 protein binding	IPI PMID:30194290 Interrogating the protein interactomes of RAS isoforms ident...	MARK AS OVER ANNOTATED	Summary: Generic protein binding is over-annotated for CHMP7: protein binding. Reason: The meaningful interactions are CHMP7-LEMD2 and CHMP7-CHMP4B; GO:0005515 is too generic to represent CHMP7 function. Supporting Evidence: PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b PMID:28242692 CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro file:human/CHMP7/CHMP7-notes.md The meaningful molecular interactions are CHMP7-LEMD2 and CHMP7-CHMP4B, not undifferentiated protein binding.
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	MARK AS OVER ANNOTATED	Summary: Generic protein binding is over-annotated for CHMP7: protein binding. Reason: The meaningful interactions are CHMP7-LEMD2 and CHMP7-CHMP4B; GO:0005515 is too generic to represent CHMP7 function. Supporting Evidence: PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b PMID:28242692 CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro file:human/CHMP7/CHMP7-notes.md The meaningful molecular interactions are CHMP7-LEMD2 and CHMP7-CHMP4B, not undifferentiated protein binding.
GO:0005654 nucleoplasm	IDA GO_REF:0000052	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: nucleoplasm. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0005829 cytosol	IDA GO_REF:0000052	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0000421 autophagosome membrane	IDA PMID:17984323 Functional multivesicular bodies are required for autophagic...	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: autophagosome membrane. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0000776 kinetochore	IDA PMID:26040712 Spastin and ESCRT-III coordinate mitotic spindle disassembly...	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: kinetochore. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0000815 ESCRT III complex	NAS PMID:36107470 Comprehensive analysis of the human ESCRT-III-MIT domain int...	ACCEPT	Summary: Supported CHMP7 ESCRT-III-associated complex annotation: ESCRT III complex. Reason: CHMP7 is an ESCRT-III-like factor that recruits and associates with downstream ESCRT-III components, especially at the reforming nuclear envelope. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway file:human/CHMP7/CHMP7-notes.md CHMP7 is an ESCRT-III-like / ESCRT-II-III hybrid protein with two literature-supported contexts
GO:0001778 plasma membrane repair	IDA PMID:24482116 ESCRT machinery is required for plasma membrane repair.	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: plasma membrane repair. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0005643 nuclear pore	IDA PMID:26040713 ESCRT-III controls nuclear envelope reformation.	MODIFY	Summary: The localization essence is nuclear-envelope recruitment, not stable membership in nuclear pore. Reason: CHMP7 localizes to reforming nuclear-envelope/chromatin-disk sites; nuclear envelope is the more accurate cellular-component term. Proposed replacements: nuclear envelope Supporting Evidence: PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope file:human/CHMP7/CHMP7-notes.md exit from mitosis, nuclear pore, chromatin location, and spindle-assembly wording should be interpreted through the specific CHMP7 role in nuclear membrane reassembly and nuclear-envelope sealing
GO:0005765 lysosomal membrane	IDA PMID:17984323 Functional multivesicular bodies are required for autophagic...	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: lysosomal membrane. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0005828 kinetochore microtubule	IDA PMID:26040712 Spastin and ESCRT-III coordinate mitotic spindle disassembly...	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: kinetochore microtubule. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0005886 plasma membrane	IDA PMID:24878737 Structure of cellular ESCRT-III spirals and their relationsh...	MARK AS OVER ANNOTATED	Summary: Plasma-membrane location/context is over-extended for CHMP7: plasma membrane. Reason: CHMP7-specific evidence localizes its core recruitment function to the reforming nuclear envelope; plasma membrane context comes from broader ESCRT/viral-budding pathway inference. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Treat viral budding annotations as over-annotated for CHMP7. file:human/CHMP7/CHMP7-notes.md the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure
GO:0006914 autophagy	IMP PMID:17984323 Functional multivesicular bodies are required for autophagic...	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: autophagy. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0006997 nucleus organization	IMP PMID:20616062 Human ESCRT-III and VPS4 proteins are required for centrosom...	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: nucleus organization. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0007080 mitotic metaphase chromosome alignment	IMP PMID:20616062 Human ESCRT-III and VPS4 proteins are required for centrosom...	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: mitotic metaphase chromosome alignment. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0030496 midbody	IDA PMID:26040712 Spastin and ESCRT-III coordinate mitotic spindle disassembly...	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: midbody. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0031468 nuclear membrane reassembly	IMP PMID:26040713 ESCRT-III controls nuclear envelope reformation.	ACCEPT	Summary: Supported core CHMP7 nuclear-envelope function: nuclear membrane reassembly. Reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for nuclear membrane closure/sealing. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery PMID:28242692 CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope UniProt:Q8WUX9 required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase file:human/CHMP7/CHMP7-notes.md the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure
GO:0032585 multivesicular body membrane	IDA PMID:16554368 The ESCRT-III subunit hVps24 is required for degradation but...	KEEP AS NON CORE	Summary: Supported but secondary CHMP7 endosomal/MVB ESCRT context: multivesicular body membrane. Reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment.
GO:0036258 multivesicular body assembly	NAS PMID:16505166 Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a...	KEEP AS NON CORE	Summary: Supported but secondary CHMP7 endosomal/MVB ESCRT context: multivesicular body assembly. Reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment.
GO:0039702 viral budding via host ESCRT complex	IDA PMID:24878737 Structure of cellular ESCRT-III spirals and their relationsh...	MARK AS OVER ANNOTATED	Summary: Over-annotated CHMP7 viral-budding term: viral budding via host ESCRT complex. Reason: The CHMP7 viral evidence is a dominant-negative overexpression effect on virus-like particle release, not an endogenous core CHMP7 viral-budding function. Supporting Evidence: PMID:16856878 dominant-negative effect of overexpressed GFP-CHMP7 file:human/CHMP7/CHMP7-notes.md The direct evidence is dominant-negative overexpression affecting virus-like particle release, not a demonstrated endogenous core viral-budding function.
GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	IDA PMID:17984323 Functional multivesicular bodies are required for autophagic...	KEEP AS NON CORE	Summary: Supported but secondary CHMP7 endosomal/MVB ESCRT context: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway. Reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment.
GO:0046761 viral budding from plasma membrane	IDA PMID:24878737 Structure of cellular ESCRT-III spirals and their relationsh...	MARK AS OVER ANNOTATED	Summary: Over-annotated CHMP7 viral-budding term: viral budding from plasma membrane. Reason: The CHMP7 viral evidence is a dominant-negative overexpression effect on virus-like particle release, not an endogenous core CHMP7 viral-budding function. Supporting Evidence: PMID:16856878 dominant-negative effect of overexpressed GFP-CHMP7 file:human/CHMP7/CHMP7-notes.md The direct evidence is dominant-negative overexpression affecting virus-like particle release, not a demonstrated endogenous core viral-budding function.
GO:0051469 vesicle fusion with vacuole	NAS PMID:16505166 Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a...	MODIFY	Summary: This fusion/vacuole annotation should be generalized to the better-supported endolysosomal route: vesicle fusion with vacuole. Reason: The available CHMP7 evidence supports endosomal sorting pathway involvement, not a specific vesicle-fusion activity. Proposed replacements: late endosome to lysosome transport Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment.
GO:0061763 multivesicular body-lysosome fusion	NAS PMID:16505166 Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a...	MODIFY	Summary: This fusion/vacuole annotation should be generalized to the better-supported endolysosomal route: multivesicular body-lysosome fusion. Reason: The available CHMP7 evidence supports endosomal sorting pathway involvement, not a specific vesicle-fusion activity. Proposed replacements: late endosome to lysosome transport Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment.
GO:0061952 midbody abscission	IMP PMID:20616062 Human ESCRT-III and VPS4 proteins are required for centrosom...	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: midbody abscission. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0071985 multivesicular body sorting pathway	IDA PMID:16554368 The ESCRT-III subunit hVps24 is required for degradation but...	KEEP AS NON CORE	Summary: Supported but secondary CHMP7 endosomal/MVB ESCRT context: multivesicular body sorting pathway. Reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment.
GO:0090148 membrane fission	NAS PMID:19234443 Membrane scission by the ESCRT-III complex.	ACCEPT	Summary: Supported ESCRT membrane-remodeling output for CHMP7 nuclear-envelope closure: membrane fission. Reason: Nuclear-envelope sealing is an ESCRT membrane-closure/fission-like process and is the best-supported CHMP7-specific core function. Supporting Evidence: PMID:26040712 ESCRT-III, VPS4 and spastin cooperate to coordinate nuclear envelope sealing and spindle disassembly PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope file:human/CHMP7/CHMP7-notes.md Accept as core: nuclear envelope, nuclear membrane reassembly, ESCRT III complex, membrane fission
GO:0097352 autophagosome maturation	IMP PMID:17984323 Functional multivesicular bodies are required for autophagic...	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: autophagosome maturation. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:1901673 regulation of mitotic spindle assembly	IMP PMID:20616062 Human ESCRT-III and VPS4 proteins are required for centrosom...	MODIFY	Summary: The cell-cycle/spindle wording is too broad for CHMP7: regulation of mitotic spindle assembly. Reason: CHMP7 contributes specifically to nuclear membrane reassembly and NE sealing during mitotic exit rather than to broad mitotic progression or spindle assembly. Proposed replacements: nuclear membrane reassembly Supporting Evidence: PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope file:human/CHMP7/CHMP7-notes.md exit from mitosis, nuclear pore, chromatin location, and spindle-assembly wording should be interpreted through the specific CHMP7 role in nuclear membrane reassembly and nuclear-envelope sealing
GO:1902774 late endosome to lysosome transport	IMP PMID:17984323 Functional multivesicular bodies are required for autophagic...	KEEP AS NON CORE	Summary: Supported but secondary CHMP7 endosomal/MVB ESCRT context: late endosome to lysosome transport. Reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment.
GO:1904930 amphisome membrane	IDA PMID:17984323 Functional multivesicular bodies are required for autophagic...	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: amphisome membrane. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0005635 nuclear envelope	EXP PMID:28242692 LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope clos...	ACCEPT	Summary: Supported core CHMP7 nuclear-envelope function: nuclear envelope. Reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for nuclear membrane closure/sealing. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery PMID:28242692 CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope UniProt:Q8WUX9 required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase file:human/CHMP7/CHMP7-notes.md the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure
GO:0005737 cytoplasm	EXP PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CH...	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: cytoplasm. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0000785 chromatin	IDA PMID:28242692 LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope clos...	MODIFY	Summary: The localization essence is nuclear-envelope recruitment, not stable membership in chromatin. Reason: CHMP7 localizes to reforming nuclear-envelope/chromatin-disk sites; nuclear envelope is the more accurate cellular-component term. Proposed replacements: nuclear envelope Supporting Evidence: PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope file:human/CHMP7/CHMP7-notes.md exit from mitosis, nuclear pore, chromatin location, and spindle-assembly wording should be interpreted through the specific CHMP7 role in nuclear membrane reassembly and nuclear-envelope sealing
GO:0031468 nuclear membrane reassembly	TAS PMID:28242692 LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope clos...	ACCEPT	Summary: Supported core CHMP7 nuclear-envelope function: nuclear membrane reassembly. Reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for nuclear membrane closure/sealing. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery PMID:28242692 CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope UniProt:Q8WUX9 required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase file:human/CHMP7/CHMP7-notes.md the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure
GO:0071168 protein localization to chromatin	IMP PMID:28242692 LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope clos...	ACCEPT	Summary: Supported CHMP7-dependent recruitment of ESCRT factors to chromatin disks/reforming NE: protein localization to chromatin. Reason: CHMP7-dependent recruitment of IST1/CHMP8 and CHMP2A to chromatin disks is part of its nuclear-envelope ESCRT recruitment function. Supporting Evidence: PMID:28242692 CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope file:human/CHMP7/CHMP7-notes.md protein localization to chromatin / chromatin disk periphery where this represents LEMD2-dependent recruitment of CHMP7 and downstream ESCRT factors to the reforming nuclear envelope
GO:0000815 ESCRT III complex	NAS PMID:28242692 LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope clos...	ACCEPT	Summary: Supported CHMP7 ESCRT-III-associated complex annotation: ESCRT III complex. Reason: CHMP7 is an ESCRT-III-like factor that recruits and associates with downstream ESCRT-III components, especially at the reforming nuclear envelope. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway file:human/CHMP7/CHMP7-notes.md CHMP7 is an ESCRT-III-like / ESCRT-II-III hybrid protein with two literature-supported contexts
GO:0005515 protein binding	IPI PMID:28242692 LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope clos...	MARK AS OVER ANNOTATED	Summary: Generic protein binding is over-annotated for CHMP7: protein binding. Reason: The meaningful interactions are CHMP7-LEMD2 and CHMP7-CHMP4B; GO:0005515 is too generic to represent CHMP7 function. Supporting Evidence: PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b PMID:28242692 CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro file:human/CHMP7/CHMP7-notes.md The meaningful molecular interactions are CHMP7-LEMD2 and CHMP7-CHMP4B, not undifferentiated protein binding.
GO:0000815 ESCRT III complex	IDA PMID:26040712 Spastin and ESCRT-III coordinate mitotic spindle disassembly...	ACCEPT	Summary: Supported CHMP7 ESCRT-III-associated complex annotation: ESCRT III complex. Reason: CHMP7 is an ESCRT-III-like factor that recruits and associates with downstream ESCRT-III components, especially at the reforming nuclear envelope. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway file:human/CHMP7/CHMP7-notes.md CHMP7 is an ESCRT-III-like / ESCRT-II-III hybrid protein with two literature-supported contexts
GO:0005635 nuclear envelope	IDA PMID:26040712 Spastin and ESCRT-III coordinate mitotic spindle disassembly...	ACCEPT	Summary: Supported core CHMP7 nuclear-envelope function: nuclear envelope. Reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for nuclear membrane closure/sealing. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery PMID:28242692 CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope UniProt:Q8WUX9 required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase file:human/CHMP7/CHMP7-notes.md the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure
GO:0010458 exit from mitosis	IMP PMID:26040712 Spastin and ESCRT-III coordinate mitotic spindle disassembly...	MODIFY	Summary: The cell-cycle/spindle wording is too broad for CHMP7: exit from mitosis. Reason: CHMP7 contributes specifically to nuclear membrane reassembly and NE sealing during mitotic exit rather than to broad mitotic progression or spindle assembly. Proposed replacements: nuclear membrane reassembly Supporting Evidence: PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope file:human/CHMP7/CHMP7-notes.md exit from mitosis, nuclear pore, chromatin location, and spindle-assembly wording should be interpreted through the specific CHMP7 role in nuclear membrane reassembly and nuclear-envelope sealing
GO:0031468 nuclear membrane reassembly	IMP PMID:26040712 Spastin and ESCRT-III coordinate mitotic spindle disassembly...	ACCEPT	Summary: Supported core CHMP7 nuclear-envelope function: nuclear membrane reassembly. Reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for nuclear membrane closure/sealing. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery PMID:28242692 CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope UniProt:Q8WUX9 required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase file:human/CHMP7/CHMP7-notes.md the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure
GO:0036258 multivesicular body assembly	NAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a...	KEEP AS NON CORE	Summary: Supported but secondary CHMP7 endosomal/MVB ESCRT context: multivesicular body assembly. Reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment.
GO:0039702 viral budding via host ESCRT complex	NAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a...	MARK AS OVER ANNOTATED	Summary: Over-annotated CHMP7 viral-budding term: viral budding via host ESCRT complex. Reason: The CHMP7 viral evidence is a dominant-negative overexpression effect on virus-like particle release, not an endogenous core CHMP7 viral-budding function. Supporting Evidence: PMID:16856878 dominant-negative effect of overexpressed GFP-CHMP7 file:human/CHMP7/CHMP7-notes.md The direct evidence is dominant-negative overexpression affecting virus-like particle release, not a demonstrated endogenous core viral-budding function.
GO:1904903 ESCRT III complex disassembly	NAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a...	KEEP AS NON CORE	Summary: Supported but secondary CHMP7 endosomal/MVB ESCRT context: ESCRT III complex disassembly. Reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment.
GO:0005829 cytosol	TAS Reactome:R-HSA-3159232	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0005829 cytosol	TAS Reactome:R-HSA-917693	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0005829 cytosol	TAS Reactome:R-HSA-917700	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0005829 cytosol	TAS Reactome:R-HSA-9668335	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0005829 cytosol	TAS Reactome:R-HSA-9668389	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0005829 cytosol	TAS Reactome:R-HSA-9668395	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0005829 cytosol	TAS Reactome:R-HSA-9668398	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0005829 cytosol	TAS Reactome:R-HSA-9668405	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0005829 cytosol	TAS Reactome:R-HSA-9668415	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0005829 cytosol	TAS Reactome:R-HSA-9668419	KEEP AS NON CORE	Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
GO:0000815 ESCRT III complex	IDA PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CH...	ACCEPT	Summary: Supported CHMP7 ESCRT-III-associated complex annotation: ESCRT III complex. Reason: CHMP7 is an ESCRT-III-like factor that recruits and associates with downstream ESCRT-III components, especially at the reforming nuclear envelope. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway file:human/CHMP7/CHMP7-notes.md CHMP7 is an ESCRT-III-like / ESCRT-II-III hybrid protein with two literature-supported contexts
GO:0045324 late endosome to vacuole transport	IMP PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CH...	MODIFY	Summary: The endosomal-sorting evidence is real, but late endosome to vacuole transport should be expressed with human MVB/endolysosomal terms. Reason: CHMP7 has an older supported endosomal-sorting phenotype, but vacuole wording is less appropriate for human curation than MVB sorting / late endosome-to-lysosome transport. Proposed replacements: multivesicular body sorting pathway late endosome to lysosome transport Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment.

📄 View Raw YAML

id: Q8WUX9
gene_symbol: CHMP7
product_type: PROTEIN
status: COMPLETE
taxon:
  id: NCBITaxon:9606
  label: Homo sapiens
description: >-
  CHMP7 encodes an ESCRT-III-like / ESCRT-II-III hybrid protein that acts most specifically as an
  upstream adaptor for nuclear-envelope ESCRT recruitment. During late anaphase, LEMD2/LEM2 recruits
  CHMP7 to the reforming nuclear envelope and chromatin-disk periphery, where CHMP7 helps recruit
  downstream ESCRT-III components such as CHMP2A and IST1/CHMP8 to seal nuclear-envelope holes and
  coordinate spindle microtubule disassembly. CHMP7 also has evidence for CHMP4B-associated endosomal
  sorting, but this appears secondary to its better-defined nuclear-envelope repair/sealing role.
alternative_products:
- name: '1'
  id: Q8WUX9-1
- name: '2'
  id: Q8WUX9-2
  sequence_note: VSP_056945, VSP_056946
existing_annotations:
- term:
    id: GO:0005635
    label: nuclear envelope
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: is_active_in
  review:
    summary: 'Supported core CHMP7 nuclear-envelope function: nuclear envelope.'
    action: ACCEPT
    reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for
      nuclear membrane closure/sealing.
    supported_by:
    - reference_id: PMID:26040712
      supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules
    - reference_id: PMID:26040712
      supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection
        sites
    - reference_id: PMID:28242692
      supporting_text: CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery
    - reference_id: PMID:28242692
      supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro
    - reference_id: PMID:28242692
      supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope
    - reference_id: UniProt:Q8WUX9
      supporting_text: required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III
        factors to the nuclear envelope for nuclear membrane closure
- term:
    id: GO:0031468
    label: nuclear membrane reassembly
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: involved_in
  review:
    summary: 'Supported core CHMP7 nuclear-envelope function: nuclear membrane reassembly.'
    action: ACCEPT
    reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for
      nuclear membrane closure/sealing.
    supported_by:
    - reference_id: PMID:26040712
      supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules
    - reference_id: PMID:26040712
      supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection
        sites
    - reference_id: PMID:28242692
      supporting_text: CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery
    - reference_id: PMID:28242692
      supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro
    - reference_id: PMID:28242692
      supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope
    - reference_id: UniProt:Q8WUX9
      supporting_text: required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III
        factors to the nuclear envelope for nuclear membrane closure
- term:
    id: GO:0000815
    label: ESCRT III complex
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: part_of
  review:
    summary: 'Supported CHMP7 ESCRT-III-associated complex annotation: ESCRT III complex.'
    action: ACCEPT
    reason: CHMP7 is an ESCRT-III-like factor that recruits and associates with downstream ESCRT-III components, especially
      at the reforming nuclear envelope.
    supported_by:
    - reference_id: PMID:26040712
      supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules
    - reference_id: PMID:28242692
      supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope
    - reference_id: PMID:16856878
      supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting
        pathway
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: CHMP7 is an ESCRT-III-like / ESCRT-II-III hybrid protein with two literature-supported contexts
- term:
    id: GO:0009898
    label: cytoplasmic side of plasma membrane
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: is_active_in
  review:
    summary: 'Plasma-membrane location/context is over-extended for CHMP7: cytoplasmic side of plasma membrane.'
    action: MARK_AS_OVER_ANNOTATED
    reason: CHMP7-specific evidence localizes its core recruitment function to the reforming nuclear envelope; plasma membrane
      context comes from broader ESCRT/viral-budding pathway inference.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Treat viral budding annotations as over-annotated for CHMP7.
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III
        factors to the nuclear envelope for nuclear membrane closure
- term:
    id: GO:0032511
    label: late endosome to vacuole transport via multivesicular body sorting pathway
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: involved_in
  review:
    summary: The endosomal-sorting evidence is real, but late endosome to vacuole transport via multivesicular body sorting
      pathway should be expressed with human MVB/endolysosomal terms.
    action: MODIFY
    reason: CHMP7 has an older supported endosomal-sorting phenotype, but vacuole wording is less appropriate for human curation
      than MVB sorting / late endosome-to-lysosome transport.
    supported_by:
    - reference_id: PMID:16856878
      supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting
        pathway
    - reference_id: PMID:16856878
      supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is
        nuclear-envelope ESCRT recruitment.
    proposed_replacement_terms:
    - id: GO:0071985
      label: multivesicular body sorting pathway
    - id: GO:1902774
      label: late endosome to lysosome transport
- term:
    id: GO:0000776
    label: kinetochore
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  qualifier: located_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: kinetochore.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0001778
    label: plasma membrane repair
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  qualifier: involved_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: plasma membrane repair.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0005635
    label: nuclear envelope
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  qualifier: located_in
  review:
    summary: 'Supported core CHMP7 nuclear-envelope function: nuclear envelope.'
    action: ACCEPT
    reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for
      nuclear membrane closure/sealing.
    supported_by:
    - reference_id: PMID:26040712
      supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules
    - reference_id: PMID:26040712
      supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection
        sites
    - reference_id: PMID:28242692
      supporting_text: CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery
    - reference_id: PMID:28242692
      supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro
    - reference_id: PMID:28242692
      supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope
    - reference_id: UniProt:Q8WUX9
      supporting_text: required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III
        factors to the nuclear envelope for nuclear membrane closure
- term:
    id: GO:0005643
    label: nuclear pore
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  qualifier: part_of
  review:
    summary: The localization essence is nuclear-envelope recruitment, not stable membership in nuclear pore.
    action: MODIFY
    reason: CHMP7 localizes to reforming nuclear-envelope/chromatin-disk sites; nuclear envelope is the more accurate cellular-component
      term.
    supported_by:
    - reference_id: PMID:26040712
      supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection
        sites
    - reference_id: PMID:28242692
      supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: exit from mitosis, nuclear pore, chromatin location, and spindle-assembly wording should be interpreted
        through the specific CHMP7 role in nuclear membrane reassembly and nuclear-envelope sealing
    proposed_replacement_terms:
    - id: GO:0005635
      label: nuclear envelope
- term:
    id: GO:0005737
    label: cytoplasm
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  qualifier: located_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: cytoplasm.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0005765
    label: lysosomal membrane
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  qualifier: located_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: lysosomal membrane.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0005828
    label: kinetochore microtubule
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  qualifier: located_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: kinetochore microtubule.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0007034
    label: vacuolar transport
  evidence_type: IEA
  original_reference_id: GO_REF:0000002
  qualifier: involved_in
  review:
    summary: The endosomal-sorting evidence is real, but vacuolar transport should be expressed with human MVB/endolysosomal
      terms.
    action: MODIFY
    reason: CHMP7 has an older supported endosomal-sorting phenotype, but vacuole wording is less appropriate for human curation
      than MVB sorting / late endosome-to-lysosome transport.
    supported_by:
    - reference_id: PMID:16856878
      supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting
        pathway
    - reference_id: PMID:16856878
      supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is
        nuclear-envelope ESCRT recruitment.
    proposed_replacement_terms:
    - id: GO:0071985
      label: multivesicular body sorting pathway
    - id: GO:1902774
      label: late endosome to lysosome transport
- term:
    id: GO:0007080
    label: mitotic metaphase chromosome alignment
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  qualifier: involved_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: mitotic metaphase chromosome alignment.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0030496
    label: midbody
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  qualifier: located_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: midbody.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0031468
    label: nuclear membrane reassembly
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  qualifier: involved_in
  review:
    summary: 'Supported core CHMP7 nuclear-envelope function: nuclear membrane reassembly.'
    action: ACCEPT
    reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for
      nuclear membrane closure/sealing.
    supported_by:
    - reference_id: PMID:26040712
      supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules
    - reference_id: PMID:26040712
      supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection
        sites
    - reference_id: PMID:28242692
      supporting_text: CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery
    - reference_id: PMID:28242692
      supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro
    - reference_id: PMID:28242692
      supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope
    - reference_id: UniProt:Q8WUX9
      supporting_text: required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III
        factors to the nuclear envelope for nuclear membrane closure
- term:
    id: GO:0032585
    label: multivesicular body membrane
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  qualifier: located_in
  review:
    summary: 'Supported but secondary CHMP7 endosomal/MVB ESCRT context: multivesicular body membrane.'
    action: KEEP_AS_NON_CORE
    reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism
      is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope.
    supported_by:
    - reference_id: PMID:16856878
      supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting
        pathway
    - reference_id: PMID:16856878
      supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is
        nuclear-envelope ESCRT recruitment.
- term:
    id: GO:0039702
    label: viral budding via host ESCRT complex
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  qualifier: involved_in
  review:
    summary: 'Over-annotated CHMP7 viral-budding term: viral budding via host ESCRT complex.'
    action: MARK_AS_OVER_ANNOTATED
    reason: The CHMP7 viral evidence is a dominant-negative overexpression effect on virus-like particle release, not an endogenous
      core CHMP7 viral-budding function.
    supported_by:
    - reference_id: PMID:16856878
      supporting_text: dominant-negative effect of overexpressed GFP-CHMP7
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: The direct evidence is dominant-negative overexpression affecting virus-like particle release, not
        a demonstrated endogenous core viral-budding function.
- term:
    id: GO:0043162
    label: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  qualifier: involved_in
  review:
    summary: 'Supported but secondary CHMP7 endosomal/MVB ESCRT context: ubiquitin-dependent protein catabolic process via
      the multivesicular body sorting pathway.'
    action: KEEP_AS_NON_CORE
    reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism
      is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope.
    supported_by:
    - reference_id: PMID:16856878
      supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting
        pathway
    - reference_id: PMID:16856878
      supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is
        nuclear-envelope ESCRT recruitment.
- term:
    id: GO:0046761
    label: viral budding from plasma membrane
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  qualifier: involved_in
  review:
    summary: 'Over-annotated CHMP7 viral-budding term: viral budding from plasma membrane.'
    action: MARK_AS_OVER_ANNOTATED
    reason: The CHMP7 viral evidence is a dominant-negative overexpression effect on virus-like particle release, not an endogenous
      core CHMP7 viral-budding function.
    supported_by:
    - reference_id: PMID:16856878
      supporting_text: dominant-negative effect of overexpressed GFP-CHMP7
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: The direct evidence is dominant-negative overexpression affecting virus-like particle release, not
        a demonstrated endogenous core viral-budding function.
- term:
    id: GO:0061952
    label: midbody abscission
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  qualifier: involved_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: midbody abscission.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0071985
    label: multivesicular body sorting pathway
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  qualifier: involved_in
  review:
    summary: 'Supported but secondary CHMP7 endosomal/MVB ESCRT context: multivesicular body sorting pathway.'
    action: KEEP_AS_NON_CORE
    reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism
      is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope.
    supported_by:
    - reference_id: PMID:16856878
      supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting
        pathway
    - reference_id: PMID:16856878
      supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is
        nuclear-envelope ESCRT recruitment.
- term:
    id: GO:0097352
    label: autophagosome maturation
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  qualifier: involved_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: autophagosome maturation.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:1901673
    label: regulation of mitotic spindle assembly
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  qualifier: involved_in
  review:
    summary: 'The cell-cycle/spindle wording is too broad for CHMP7: regulation of mitotic spindle assembly.'
    action: MODIFY
    reason: CHMP7 contributes specifically to nuclear membrane reassembly and NE sealing during mitotic exit rather than to
      broad mitotic progression or spindle assembly.
    supported_by:
    - reference_id: PMID:26040712
      supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection
        sites
    - reference_id: PMID:28242692
      supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: exit from mitosis, nuclear pore, chromatin location, and spindle-assembly wording should be interpreted
        through the specific CHMP7 role in nuclear membrane reassembly and nuclear-envelope sealing
    proposed_replacement_terms:
    - id: GO:0031468
      label: nuclear membrane reassembly
- term:
    id: GO:1902774
    label: late endosome to lysosome transport
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  qualifier: involved_in
  review:
    summary: 'Supported but secondary CHMP7 endosomal/MVB ESCRT context: late endosome to lysosome transport.'
    action: KEEP_AS_NON_CORE
    reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism
      is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope.
    supported_by:
    - reference_id: PMID:16856878
      supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting
        pathway
    - reference_id: PMID:16856878
      supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is
        nuclear-envelope ESCRT recruitment.
- term:
    id: GO:1904930
    label: amphisome membrane
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  qualifier: located_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: amphisome membrane.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:16856878
  qualifier: enables
  review:
    summary: 'Generic protein binding is over-annotated for CHMP7: protein binding.'
    action: MARK_AS_OVER_ANNOTATED
    reason: The meaningful interactions are CHMP7-LEMD2 and CHMP7-CHMP4B; GO:0005515 is too generic to represent CHMP7 function.
    supported_by:
    - reference_id: PMID:16856878
      supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b
    - reference_id: PMID:28242692
      supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: The meaningful molecular interactions are CHMP7-LEMD2 and CHMP7-CHMP4B, not undifferentiated protein
        binding.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:30194290
  qualifier: enables
  review:
    summary: 'Generic protein binding is over-annotated for CHMP7: protein binding.'
    action: MARK_AS_OVER_ANNOTATED
    reason: The meaningful interactions are CHMP7-LEMD2 and CHMP7-CHMP4B; GO:0005515 is too generic to represent CHMP7 function.
    supported_by:
    - reference_id: PMID:16856878
      supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b
    - reference_id: PMID:28242692
      supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: The meaningful molecular interactions are CHMP7-LEMD2 and CHMP7-CHMP4B, not undifferentiated protein
        binding.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:32814053
  qualifier: enables
  review:
    summary: 'Generic protein binding is over-annotated for CHMP7: protein binding.'
    action: MARK_AS_OVER_ANNOTATED
    reason: The meaningful interactions are CHMP7-LEMD2 and CHMP7-CHMP4B; GO:0005515 is too generic to represent CHMP7 function.
    supported_by:
    - reference_id: PMID:16856878
      supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b
    - reference_id: PMID:28242692
      supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: The meaningful molecular interactions are CHMP7-LEMD2 and CHMP7-CHMP4B, not undifferentiated protein
        binding.
- term:
    id: GO:0005654
    label: nucleoplasm
  evidence_type: IDA
  original_reference_id: GO_REF:0000052
  qualifier: located_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: nucleoplasm.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: IDA
  original_reference_id: GO_REF:0000052
  qualifier: located_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0000421
    label: autophagosome membrane
  evidence_type: IDA
  original_reference_id: PMID:17984323
  qualifier: located_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: autophagosome membrane.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0000776
    label: kinetochore
  evidence_type: IDA
  original_reference_id: PMID:26040712
  qualifier: located_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: kinetochore.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0000815
    label: ESCRT III complex
  evidence_type: NAS
  original_reference_id: PMID:36107470
  qualifier: part_of
  review:
    summary: 'Supported CHMP7 ESCRT-III-associated complex annotation: ESCRT III complex.'
    action: ACCEPT
    reason: CHMP7 is an ESCRT-III-like factor that recruits and associates with downstream ESCRT-III components, especially
      at the reforming nuclear envelope.
    supported_by:
    - reference_id: PMID:26040712
      supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules
    - reference_id: PMID:28242692
      supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope
    - reference_id: PMID:16856878
      supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting
        pathway
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: CHMP7 is an ESCRT-III-like / ESCRT-II-III hybrid protein with two literature-supported contexts
- term:
    id: GO:0001778
    label: plasma membrane repair
  evidence_type: IDA
  original_reference_id: PMID:24482116
  qualifier: involved_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: plasma membrane repair.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0005643
    label: nuclear pore
  evidence_type: IDA
  original_reference_id: PMID:26040713
  qualifier: part_of
  review:
    summary: The localization essence is nuclear-envelope recruitment, not stable membership in nuclear pore.
    action: MODIFY
    reason: CHMP7 localizes to reforming nuclear-envelope/chromatin-disk sites; nuclear envelope is the more accurate cellular-component
      term.
    supported_by:
    - reference_id: PMID:26040712
      supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection
        sites
    - reference_id: PMID:28242692
      supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: exit from mitosis, nuclear pore, chromatin location, and spindle-assembly wording should be interpreted
        through the specific CHMP7 role in nuclear membrane reassembly and nuclear-envelope sealing
    proposed_replacement_terms:
    - id: GO:0005635
      label: nuclear envelope
- term:
    id: GO:0005765
    label: lysosomal membrane
  evidence_type: IDA
  original_reference_id: PMID:17984323
  qualifier: located_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: lysosomal membrane.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0005828
    label: kinetochore microtubule
  evidence_type: IDA
  original_reference_id: PMID:26040712
  qualifier: located_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: kinetochore microtubule.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0005886
    label: plasma membrane
  evidence_type: IDA
  original_reference_id: PMID:24878737
  qualifier: located_in
  review:
    summary: 'Plasma-membrane location/context is over-extended for CHMP7: plasma membrane.'
    action: MARK_AS_OVER_ANNOTATED
    reason: CHMP7-specific evidence localizes its core recruitment function to the reforming nuclear envelope; plasma membrane
      context comes from broader ESCRT/viral-budding pathway inference.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Treat viral budding annotations as over-annotated for CHMP7.
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III
        factors to the nuclear envelope for nuclear membrane closure
- term:
    id: GO:0006914
    label: autophagy
  evidence_type: IMP
  original_reference_id: PMID:17984323
  qualifier: involved_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: autophagy.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0006997
    label: nucleus organization
  evidence_type: IMP
  original_reference_id: PMID:20616062
  qualifier: involved_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: nucleus organization.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0007080
    label: mitotic metaphase chromosome alignment
  evidence_type: IMP
  original_reference_id: PMID:20616062
  qualifier: involved_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: mitotic metaphase chromosome alignment.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0030496
    label: midbody
  evidence_type: IDA
  original_reference_id: PMID:26040712
  qualifier: located_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: midbody.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0031468
    label: nuclear membrane reassembly
  evidence_type: IMP
  original_reference_id: PMID:26040713
  qualifier: involved_in
  review:
    summary: 'Supported core CHMP7 nuclear-envelope function: nuclear membrane reassembly.'
    action: ACCEPT
    reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for
      nuclear membrane closure/sealing.
    supported_by:
    - reference_id: PMID:26040712
      supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules
    - reference_id: PMID:26040712
      supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection
        sites
    - reference_id: PMID:28242692
      supporting_text: CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery
    - reference_id: PMID:28242692
      supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro
    - reference_id: PMID:28242692
      supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope
    - reference_id: UniProt:Q8WUX9
      supporting_text: required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III
        factors to the nuclear envelope for nuclear membrane closure
- term:
    id: GO:0032585
    label: multivesicular body membrane
  evidence_type: IDA
  original_reference_id: PMID:16554368
  qualifier: located_in
  review:
    summary: 'Supported but secondary CHMP7 endosomal/MVB ESCRT context: multivesicular body membrane.'
    action: KEEP_AS_NON_CORE
    reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism
      is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope.
    supported_by:
    - reference_id: PMID:16856878
      supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting
        pathway
    - reference_id: PMID:16856878
      supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is
        nuclear-envelope ESCRT recruitment.
- term:
    id: GO:0036258
    label: multivesicular body assembly
  evidence_type: NAS
  original_reference_id: PMID:16505166
  qualifier: involved_in
  review:
    summary: 'Supported but secondary CHMP7 endosomal/MVB ESCRT context: multivesicular body assembly.'
    action: KEEP_AS_NON_CORE
    reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism
      is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope.
    supported_by:
    - reference_id: PMID:16856878
      supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting
        pathway
    - reference_id: PMID:16856878
      supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is
        nuclear-envelope ESCRT recruitment.
- term:
    id: GO:0039702
    label: viral budding via host ESCRT complex
  evidence_type: IDA
  original_reference_id: PMID:24878737
  qualifier: involved_in
  review:
    summary: 'Over-annotated CHMP7 viral-budding term: viral budding via host ESCRT complex.'
    action: MARK_AS_OVER_ANNOTATED
    reason: The CHMP7 viral evidence is a dominant-negative overexpression effect on virus-like particle release, not an endogenous
      core CHMP7 viral-budding function.
    supported_by:
    - reference_id: PMID:16856878
      supporting_text: dominant-negative effect of overexpressed GFP-CHMP7
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: The direct evidence is dominant-negative overexpression affecting virus-like particle release, not
        a demonstrated endogenous core viral-budding function.
- term:
    id: GO:0043162
    label: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
  evidence_type: IDA
  original_reference_id: PMID:17984323
  qualifier: involved_in
  review:
    summary: 'Supported but secondary CHMP7 endosomal/MVB ESCRT context: ubiquitin-dependent protein catabolic process via
      the multivesicular body sorting pathway.'
    action: KEEP_AS_NON_CORE
    reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism
      is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope.
    supported_by:
    - reference_id: PMID:16856878
      supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting
        pathway
    - reference_id: PMID:16856878
      supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is
        nuclear-envelope ESCRT recruitment.
- term:
    id: GO:0046761
    label: viral budding from plasma membrane
  evidence_type: IDA
  original_reference_id: PMID:24878737
  qualifier: involved_in
  review:
    summary: 'Over-annotated CHMP7 viral-budding term: viral budding from plasma membrane.'
    action: MARK_AS_OVER_ANNOTATED
    reason: The CHMP7 viral evidence is a dominant-negative overexpression effect on virus-like particle release, not an endogenous
      core CHMP7 viral-budding function.
    supported_by:
    - reference_id: PMID:16856878
      supporting_text: dominant-negative effect of overexpressed GFP-CHMP7
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: The direct evidence is dominant-negative overexpression affecting virus-like particle release, not
        a demonstrated endogenous core viral-budding function.
- term:
    id: GO:0051469
    label: vesicle fusion with vacuole
  evidence_type: NAS
  original_reference_id: PMID:16505166
  qualifier: involved_in
  review:
    summary: 'This fusion/vacuole annotation should be generalized to the better-supported endolysosomal route: vesicle fusion
      with vacuole.'
    action: MODIFY
    reason: The available CHMP7 evidence supports endosomal sorting pathway involvement, not a specific vesicle-fusion activity.
    supported_by:
    - reference_id: PMID:16856878
      supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting
        pathway
    - reference_id: PMID:16856878
      supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is
        nuclear-envelope ESCRT recruitment.
    proposed_replacement_terms:
    - id: GO:1902774
      label: late endosome to lysosome transport
- term:
    id: GO:0061763
    label: multivesicular body-lysosome fusion
  evidence_type: NAS
  original_reference_id: PMID:16505166
  qualifier: involved_in
  review:
    summary: 'This fusion/vacuole annotation should be generalized to the better-supported endolysosomal route: multivesicular
      body-lysosome fusion.'
    action: MODIFY
    reason: The available CHMP7 evidence supports endosomal sorting pathway involvement, not a specific vesicle-fusion activity.
    supported_by:
    - reference_id: PMID:16856878
      supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting
        pathway
    - reference_id: PMID:16856878
      supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is
        nuclear-envelope ESCRT recruitment.
    proposed_replacement_terms:
    - id: GO:1902774
      label: late endosome to lysosome transport
- term:
    id: GO:0061952
    label: midbody abscission
  evidence_type: IMP
  original_reference_id: PMID:20616062
  qualifier: involved_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: midbody abscission.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0071985
    label: multivesicular body sorting pathway
  evidence_type: IDA
  original_reference_id: PMID:16554368
  qualifier: involved_in
  review:
    summary: 'Supported but secondary CHMP7 endosomal/MVB ESCRT context: multivesicular body sorting pathway.'
    action: KEEP_AS_NON_CORE
    reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism
      is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope.
    supported_by:
    - reference_id: PMID:16856878
      supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting
        pathway
    - reference_id: PMID:16856878
      supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is
        nuclear-envelope ESCRT recruitment.
- term:
    id: GO:0090148
    label: membrane fission
  evidence_type: NAS
  original_reference_id: PMID:19234443
  qualifier: involved_in
  review:
    summary: 'Supported ESCRT membrane-remodeling output for CHMP7 nuclear-envelope closure: membrane fission.'
    action: ACCEPT
    reason: Nuclear-envelope sealing is an ESCRT membrane-closure/fission-like process and is the best-supported CHMP7-specific
      core function.
    supported_by:
    - reference_id: PMID:26040712
      supporting_text: ESCRT-III, VPS4 and spastin cooperate to coordinate nuclear envelope sealing and spindle disassembly
    - reference_id: PMID:28242692
      supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: 'Accept as core: nuclear envelope, nuclear membrane reassembly, ESCRT III complex, membrane fission'
- term:
    id: GO:0097352
    label: autophagosome maturation
  evidence_type: IMP
  original_reference_id: PMID:17984323
  qualifier: involved_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: autophagosome maturation.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:1901673
    label: regulation of mitotic spindle assembly
  evidence_type: IMP
  original_reference_id: PMID:20616062
  qualifier: involved_in
  review:
    summary: 'The cell-cycle/spindle wording is too broad for CHMP7: regulation of mitotic spindle assembly.'
    action: MODIFY
    reason: CHMP7 contributes specifically to nuclear membrane reassembly and NE sealing during mitotic exit rather than to
      broad mitotic progression or spindle assembly.
    supported_by:
    - reference_id: PMID:26040712
      supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection
        sites
    - reference_id: PMID:28242692
      supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: exit from mitosis, nuclear pore, chromatin location, and spindle-assembly wording should be interpreted
        through the specific CHMP7 role in nuclear membrane reassembly and nuclear-envelope sealing
    proposed_replacement_terms:
    - id: GO:0031468
      label: nuclear membrane reassembly
- term:
    id: GO:1902774
    label: late endosome to lysosome transport
  evidence_type: IMP
  original_reference_id: PMID:17984323
  qualifier: involved_in
  review:
    summary: 'Supported but secondary CHMP7 endosomal/MVB ESCRT context: late endosome to lysosome transport.'
    action: KEEP_AS_NON_CORE
    reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism
      is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope.
    supported_by:
    - reference_id: PMID:16856878
      supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting
        pathway
    - reference_id: PMID:16856878
      supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is
        nuclear-envelope ESCRT recruitment.
- term:
    id: GO:1904930
    label: amphisome membrane
  evidence_type: IDA
  original_reference_id: PMID:17984323
  qualifier: located_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: amphisome membrane.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0005635
    label: nuclear envelope
  evidence_type: EXP
  original_reference_id: PMID:28242692
  qualifier: located_in
  review:
    summary: 'Supported core CHMP7 nuclear-envelope function: nuclear envelope.'
    action: ACCEPT
    reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for
      nuclear membrane closure/sealing.
    supported_by:
    - reference_id: PMID:26040712
      supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules
    - reference_id: PMID:26040712
      supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection
        sites
    - reference_id: PMID:28242692
      supporting_text: CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery
    - reference_id: PMID:28242692
      supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro
    - reference_id: PMID:28242692
      supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope
    - reference_id: UniProt:Q8WUX9
      supporting_text: required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III
        factors to the nuclear envelope for nuclear membrane closure
- term:
    id: GO:0005737
    label: cytoplasm
  evidence_type: EXP
  original_reference_id: PMID:16856878
  qualifier: located_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: cytoplasm.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0000785
    label: chromatin
  evidence_type: IDA
  original_reference_id: PMID:28242692
  qualifier: located_in
  review:
    summary: The localization essence is nuclear-envelope recruitment, not stable membership in chromatin.
    action: MODIFY
    reason: CHMP7 localizes to reforming nuclear-envelope/chromatin-disk sites; nuclear envelope is the more accurate cellular-component
      term.
    supported_by:
    - reference_id: PMID:26040712
      supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection
        sites
    - reference_id: PMID:28242692
      supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: exit from mitosis, nuclear pore, chromatin location, and spindle-assembly wording should be interpreted
        through the specific CHMP7 role in nuclear membrane reassembly and nuclear-envelope sealing
    proposed_replacement_terms:
    - id: GO:0005635
      label: nuclear envelope
- term:
    id: GO:0031468
    label: nuclear membrane reassembly
  evidence_type: TAS
  original_reference_id: PMID:28242692
  qualifier: involved_in
  review:
    summary: 'Supported core CHMP7 nuclear-envelope function: nuclear membrane reassembly.'
    action: ACCEPT
    reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for
      nuclear membrane closure/sealing.
    supported_by:
    - reference_id: PMID:26040712
      supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules
    - reference_id: PMID:26040712
      supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection
        sites
    - reference_id: PMID:28242692
      supporting_text: CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery
    - reference_id: PMID:28242692
      supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro
    - reference_id: PMID:28242692
      supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope
    - reference_id: UniProt:Q8WUX9
      supporting_text: required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III
        factors to the nuclear envelope for nuclear membrane closure
- term:
    id: GO:0071168
    label: protein localization to chromatin
  evidence_type: IMP
  original_reference_id: PMID:28242692
  qualifier: involved_in
  review:
    summary: 'Supported CHMP7-dependent recruitment of ESCRT factors to chromatin disks/reforming NE: protein localization
      to chromatin.'
    action: ACCEPT
    reason: CHMP7-dependent recruitment of IST1/CHMP8 and CHMP2A to chromatin disks is part of its nuclear-envelope ESCRT
      recruitment function.
    supported_by:
    - reference_id: PMID:28242692
      supporting_text: CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery
    - reference_id: PMID:28242692
      supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: protein localization to chromatin / chromatin disk periphery where this represents LEMD2-dependent
        recruitment of CHMP7 and downstream ESCRT factors to the reforming nuclear envelope
- term:
    id: GO:0000815
    label: ESCRT III complex
  evidence_type: NAS
  original_reference_id: PMID:28242692
  qualifier: part_of
  review:
    summary: 'Supported CHMP7 ESCRT-III-associated complex annotation: ESCRT III complex.'
    action: ACCEPT
    reason: CHMP7 is an ESCRT-III-like factor that recruits and associates with downstream ESCRT-III components, especially
      at the reforming nuclear envelope.
    supported_by:
    - reference_id: PMID:26040712
      supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules
    - reference_id: PMID:28242692
      supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope
    - reference_id: PMID:16856878
      supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting
        pathway
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: CHMP7 is an ESCRT-III-like / ESCRT-II-III hybrid protein with two literature-supported contexts
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:28242692
  qualifier: enables
  review:
    summary: 'Generic protein binding is over-annotated for CHMP7: protein binding.'
    action: MARK_AS_OVER_ANNOTATED
    reason: The meaningful interactions are CHMP7-LEMD2 and CHMP7-CHMP4B; GO:0005515 is too generic to represent CHMP7 function.
    supported_by:
    - reference_id: PMID:16856878
      supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b
    - reference_id: PMID:28242692
      supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: The meaningful molecular interactions are CHMP7-LEMD2 and CHMP7-CHMP4B, not undifferentiated protein
        binding.
- term:
    id: GO:0000815
    label: ESCRT III complex
  evidence_type: IDA
  original_reference_id: PMID:26040712
  qualifier: part_of
  review:
    summary: 'Supported CHMP7 ESCRT-III-associated complex annotation: ESCRT III complex.'
    action: ACCEPT
    reason: CHMP7 is an ESCRT-III-like factor that recruits and associates with downstream ESCRT-III components, especially
      at the reforming nuclear envelope.
    supported_by:
    - reference_id: PMID:26040712
      supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules
    - reference_id: PMID:28242692
      supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope
    - reference_id: PMID:16856878
      supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting
        pathway
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: CHMP7 is an ESCRT-III-like / ESCRT-II-III hybrid protein with two literature-supported contexts
- term:
    id: GO:0005635
    label: nuclear envelope
  evidence_type: IDA
  original_reference_id: PMID:26040712
  qualifier: located_in
  review:
    summary: 'Supported core CHMP7 nuclear-envelope function: nuclear envelope.'
    action: ACCEPT
    reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for
      nuclear membrane closure/sealing.
    supported_by:
    - reference_id: PMID:26040712
      supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules
    - reference_id: PMID:26040712
      supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection
        sites
    - reference_id: PMID:28242692
      supporting_text: CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery
    - reference_id: PMID:28242692
      supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro
    - reference_id: PMID:28242692
      supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope
    - reference_id: UniProt:Q8WUX9
      supporting_text: required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III
        factors to the nuclear envelope for nuclear membrane closure
- term:
    id: GO:0010458
    label: exit from mitosis
  evidence_type: IMP
  original_reference_id: PMID:26040712
  qualifier: involved_in
  review:
    summary: 'The cell-cycle/spindle wording is too broad for CHMP7: exit from mitosis.'
    action: MODIFY
    reason: CHMP7 contributes specifically to nuclear membrane reassembly and NE sealing during mitotic exit rather than to
      broad mitotic progression or spindle assembly.
    supported_by:
    - reference_id: PMID:26040712
      supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection
        sites
    - reference_id: PMID:28242692
      supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: exit from mitosis, nuclear pore, chromatin location, and spindle-assembly wording should be interpreted
        through the specific CHMP7 role in nuclear membrane reassembly and nuclear-envelope sealing
    proposed_replacement_terms:
    - id: GO:0031468
      label: nuclear membrane reassembly
- term:
    id: GO:0031468
    label: nuclear membrane reassembly
  evidence_type: IMP
  original_reference_id: PMID:26040712
  qualifier: involved_in
  review:
    summary: 'Supported core CHMP7 nuclear-envelope function: nuclear membrane reassembly.'
    action: ACCEPT
    reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for
      nuclear membrane closure/sealing.
    supported_by:
    - reference_id: PMID:26040712
      supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules
    - reference_id: PMID:26040712
      supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection
        sites
    - reference_id: PMID:28242692
      supporting_text: CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery
    - reference_id: PMID:28242692
      supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro
    - reference_id: PMID:28242692
      supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope
    - reference_id: UniProt:Q8WUX9
      supporting_text: required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III
        factors to the nuclear envelope for nuclear membrane closure
- term:
    id: GO:0036258
    label: multivesicular body assembly
  evidence_type: NAS
  original_reference_id: PMID:20588296
  qualifier: involved_in
  review:
    summary: 'Supported but secondary CHMP7 endosomal/MVB ESCRT context: multivesicular body assembly.'
    action: KEEP_AS_NON_CORE
    reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism
      is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope.
    supported_by:
    - reference_id: PMID:16856878
      supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting
        pathway
    - reference_id: PMID:16856878
      supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is
        nuclear-envelope ESCRT recruitment.
- term:
    id: GO:0039702
    label: viral budding via host ESCRT complex
  evidence_type: NAS
  original_reference_id: PMID:20588296
  qualifier: involved_in
  review:
    summary: 'Over-annotated CHMP7 viral-budding term: viral budding via host ESCRT complex.'
    action: MARK_AS_OVER_ANNOTATED
    reason: The CHMP7 viral evidence is a dominant-negative overexpression effect on virus-like particle release, not an endogenous
      core CHMP7 viral-budding function.
    supported_by:
    - reference_id: PMID:16856878
      supporting_text: dominant-negative effect of overexpressed GFP-CHMP7
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: The direct evidence is dominant-negative overexpression affecting virus-like particle release, not
        a demonstrated endogenous core viral-budding function.
- term:
    id: GO:1904903
    label: ESCRT III complex disassembly
  evidence_type: NAS
  original_reference_id: PMID:20588296
  qualifier: involved_in
  review:
    summary: 'Supported but secondary CHMP7 endosomal/MVB ESCRT context: ESCRT III complex disassembly.'
    action: KEEP_AS_NON_CORE
    reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism
      is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope.
    supported_by:
    - reference_id: PMID:16856878
      supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting
        pathway
    - reference_id: PMID:16856878
      supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is
        nuclear-envelope ESCRT recruitment.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-3159232
  qualifier: located_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-917693
  qualifier: located_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-917700
  qualifier: located_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9668335
  qualifier: located_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9668389
  qualifier: located_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9668395
  qualifier: located_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9668398
  qualifier: located_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9668405
  qualifier: located_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9668415
  qualifier: located_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9668419
  qualifier: located_in
  review:
    summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol.'
    action: KEEP_AS_NON_CORE
    reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific
      or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing.
    supported_by:
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm,
        kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts
- term:
    id: GO:0000815
    label: ESCRT III complex
  evidence_type: IDA
  original_reference_id: PMID:16856878
  qualifier: part_of
  review:
    summary: 'Supported CHMP7 ESCRT-III-associated complex annotation: ESCRT III complex.'
    action: ACCEPT
    reason: CHMP7 is an ESCRT-III-like factor that recruits and associates with downstream ESCRT-III components, especially
      at the reforming nuclear envelope.
    supported_by:
    - reference_id: PMID:26040712
      supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules
    - reference_id: PMID:28242692
      supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope
    - reference_id: PMID:16856878
      supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting
        pathway
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: CHMP7 is an ESCRT-III-like / ESCRT-II-III hybrid protein with two literature-supported contexts
- term:
    id: GO:0045324
    label: late endosome to vacuole transport
  evidence_type: IMP
  original_reference_id: PMID:16856878
  qualifier: involved_in
  review:
    summary: The endosomal-sorting evidence is real, but late endosome to vacuole transport should be expressed with human
      MVB/endolysosomal terms.
    action: MODIFY
    reason: CHMP7 has an older supported endosomal-sorting phenotype, but vacuole wording is less appropriate for human curation
      than MVB sorting / late endosome-to-lysosome transport.
    supported_by:
    - reference_id: PMID:16856878
      supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting
        pathway
    - reference_id: PMID:16856878
      supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b
    - reference_id: file:human/CHMP7/CHMP7-notes.md
      supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is
        nuclear-envelope ESCRT recruitment.
    proposed_replacement_terms:
    - id: GO:0071985
      label: multivesicular body sorting pathway
    - id: GO:1902774
      label: late endosome to lysosome transport
references:
- id: GO_REF:0000002
  title: Gene Ontology annotation through association of InterPro records with GO terms
  findings: []
- id: GO_REF:0000033
  title: Annotation inferences using phylogenetic trees
  findings: []
- id: GO_REF:0000044
  title: Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative
    changes to GO terms applied by UniProt
  findings: []
- id: GO_REF:0000052
  title: Gene Ontology annotation based on curation of immunofluorescence data
  findings: []
- id: GO_REF:0000117
  title: Electronic Gene Ontology annotations created by ARBA machine learning models
  findings: []
- id: PMID:16505166
  title: Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a conserved VSL region in Vta1.
  findings: []
- id: PMID:16554368
  title: The ESCRT-III subunit hVps24 is required for degradation but not silencing of the epidermal growth factor receptor.
  findings: []
- id: PMID:16856878
  title: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway.
  findings: []
- id: PMID:17984323
  title: Functional multivesicular bodies are required for autophagic clearance of protein aggregates associated with neurodegenerative
    disease.
  findings: []
- id: PMID:19234443
  title: Membrane scission by the ESCRT-III complex.
  findings: []
- id: PMID:20588296
  title: 'Membrane budding and scission by the ESCRT machinery: it''s all in the neck.'
  findings: []
- id: PMID:20616062
  title: Human ESCRT-III and VPS4 proteins are required for centrosome and spindle maintenance.
  findings: []
- id: PMID:24482116
  title: ESCRT machinery is required for plasma membrane repair.
  findings: []
- id: PMID:24878737
  title: Structure of cellular ESCRT-III spirals and their relationship to HIV budding.
  findings: []
- id: PMID:26040712
  title: Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing.
  findings: []
- id: PMID:26040713
  title: ESCRT-III controls nuclear envelope reformation.
  findings: []
- id: PMID:28242692
  title: LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells.
  findings: []
- id: PMID:30194290
  title: Interrogating the protein interactomes of RAS isoforms identifies PIP5K1A as a KRAS-specific vulnerability.
  findings: []
- id: PMID:32814053
  title: Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation
    in Affected Brains.
  findings: []
- id: PMID:36107470
  title: Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission.
  findings: []
- id: Reactome:R-HSA-3159232
  title: Recruitment Of HIV Virion Budding Machinery
  findings: []
- id: Reactome:R-HSA-917693
  title: ESCRT Disassembly
  findings: []
- id: Reactome:R-HSA-917700
  title: MVB Vesicle Formation
  findings: []
- id: Reactome:R-HSA-9668335
  title: CHMP7 binds LEMD2
  findings: []
- id: Reactome:R-HSA-9668389
  title: VPS4 binds ESCRT-III assemblies at nuclear envelope (NE) fenestrations
  findings: []
- id: Reactome:R-HSA-9668395
  title: CHMP7 binds CC2D1B
  findings: []
- id: Reactome:R-HSA-9668398
  title: CHMP7 binds CHMP4B, which recruits other subunits of the ESCRT-III complex
  findings: []
- id: Reactome:R-HSA-9668405
  title: SPAST (spastin) binds the IST1 subunit of ESCRT-III at the sites of microtubule attachment to chromatin
  findings: []
- id: Reactome:R-HSA-9668415
  title: VPS4 mediates disassembly of ESCRTIII subunits to promote sealing of holes in the nuclear envelope
  findings: []
- id: Reactome:R-HSA-9668419
  title: SPAST (spastin) mediates the severing of microtubules at chromosome attachment sites
  findings: []
- id: UniProt:Q8WUX9
  title: UniProtKB entry Q8WUX9 (CHMP7)
  findings:
  - statement: CHMP7 recruits ESCRT-III to the nuclear envelope during late anaphase and is recruited there by LEMD2.
    supporting_text: required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase
- id: file:human/CHMP7/CHMP7-notes.md
  title: CHMP7 review notes
  findings:
  - statement: CHMP7 core function is LEMD2-dependent recruitment of ESCRT factors to the reforming nuclear envelope; endosomal
      sorting is supported but secondary.
    supporting_text: the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III
      factors to the nuclear envelope for nuclear membrane closure
core_functions:
- description: CHMP7 is a LEMD2/LEM2-recruited ESCRT-III-like adaptor that localizes to reforming nuclear-envelope/chromatin-disk
    sites during late anaphase. It recruits downstream ESCRT-III factors to nuclear-envelope holes, supporting membrane closure/fission-like
    sealing of the nuclear envelope and coordination with spindle microtubule disassembly.
  in_complex:
    id: GO:0000815
    label: ESCRT III complex
  directly_involved_in:
  - id: GO:0031468
    label: nuclear membrane reassembly
  - id: GO:0071168
    label: protein localization to chromatin
  - id: GO:0090148
    label: membrane fission
  locations:
  - id: GO:0005635
    label: nuclear envelope
  supported_by:
  - reference_id: PMID:26040712
    supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules
  - reference_id: PMID:26040712
    supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection
      sites
  - reference_id: PMID:28242692
    supporting_text: CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery
  - reference_id: PMID:28242692
    supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro
  - reference_id: PMID:28242692
    supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope
  - reference_id: UniProt:Q8WUX9
    supporting_text: required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase
  - reference_id: file:human/CHMP7/CHMP7-notes.md
    supporting_text: the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III
      factors to the nuclear envelope for nuclear membrane closure
  - reference_id: PMID:26040712
    supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules
  - reference_id: PMID:28242692
    supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope
  - reference_id: PMID:16856878
    supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting
      pathway
  - reference_id: file:human/CHMP7/CHMP7-notes.md
    supporting_text: CHMP7 is an ESCRT-III-like / ESCRT-II-III hybrid protein with two literature-supported contexts
  - reference_id: PMID:28242692
    supporting_text: CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery
  - reference_id: PMID:28242692
    supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope
  - reference_id: file:human/CHMP7/CHMP7-notes.md
    supporting_text: protein localization to chromatin / chromatin disk periphery where this represents LEMD2-dependent recruitment
      of CHMP7 and downstream ESCRT factors to the reforming nuclear envelope
proposed_new_terms: []
suggested_questions:
- question: Can endogenous CHMP7 rescue assays separate LEMD2 binding, CHMP4B recruitment, and VPS4-dependent ESCRT turnover
    during nuclear-envelope sealing?
- question: Should CHMP7 endosomal-sorting annotations remain non-core unless supported by endogenous depletion/rescue assays
    distinct from dominant-negative overexpression phenotypes?
- question: Is there a more specific GO process term needed for ESCRT-mediated nuclear-envelope hole sealing during anaphase?
suggested_experiments:
- description: Rescue CHMP7-depleted cells with wild-type CHMP7 and mutants separating LEMD2 binding, CHMP4B binding, and
    ESCRT-III recruitment while measuring CHMP2A/IST1 recruitment, NE sealing reporters, and post-mitotic nuclear leakage.
  hypothesis: CHMP7 nuclear-envelope sealing will require LEMD2-dependent recruitment and downstream ESCRT-III assembly more
    directly than its endosomal-sorting phenotype.
- description: Compare acute endogenous CHMP7 depletion/rescue in EGFR or ubiquitinated-cargo MVB sorting assays versus nuclear-envelope
    sealing assays under matched expression levels.
  hypothesis: The nuclear-envelope phenotype will be the clearest CHMP7-specific core function, whereas endosomal sorting
    will be weaker or context-dependent.
- description: Test whether CHMP7 perturbation affects virus-like particle release only through overexpression/dominant-negative
    ESCRT disruption or through a reproducible endogenous requirement.
  hypothesis: Viral-budding effects will reflect pathway-level ESCRT perturbation rather than a direct core CHMP7 function.

Gene Description

Existing Annotations Review

Core Functions

References

Suggested Questions for Experts

Suggested Experiments

📚 Additional Documentation

Notes

CHMP7 notes

Review focus

Direct evidence

Annotation decisions

Description cleanup note

Pn Notes

CHMP7 PN Consistency Notes

Source Files Checked

Deep Research Files

AIGR Review Snapshot

PN Consistency Summary

Full Consistency Review

PN Dossier Context

PN row 1: Autophagy-Lysosome Pathway | Autophagosome closure maturation and lysosome fusion | Sealing of autophagophore membrane | ESCRT-III complex component

PN row 2: Autophagy-Lysosome Pathway | Microautophagy | General microautophagy machinery | ESCRT-III complex component

Projected GO annotations (3)

Note

📄 View Raw YAML