CHMP7 encodes an ESCRT-III-like / ESCRT-II-III hybrid protein that acts most specifically as an upstream adaptor for nuclear-envelope ESCRT recruitment. During late anaphase, LEMD2/LEM2 recruits CHMP7 to the reforming nuclear envelope and chromatin-disk periphery, where CHMP7 helps recruit downstream ESCRT-III components such as CHMP2A and IST1/CHMP8 to seal nuclear-envelope holes and coordinate spindle microtubule disassembly. CHMP7 also has evidence for CHMP4B-associated endosomal sorting, but this appears secondary to its better-defined nuclear-envelope repair/sealing role.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005635 nuclear envelope | IBA GO_REF:0000033 | ACCEPT | Summary: Supported core CHMP7 nuclear-envelope function: nuclear envelope. Reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for nuclear membrane closure/sealing. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery PMID:28242692 CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope UniProt:Q8WUX9 required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase file:human/CHMP7/CHMP7-notes.md the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure |
| GO:0031468 nuclear membrane reassembly | IBA GO_REF:0000033 | ACCEPT | Summary: Supported core CHMP7 nuclear-envelope function: nuclear membrane reassembly. Reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for nuclear membrane closure/sealing. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery PMID:28242692 CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope UniProt:Q8WUX9 required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase file:human/CHMP7/CHMP7-notes.md the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure |
| GO:0000815 ESCRT III complex | IBA GO_REF:0000033 | ACCEPT | Summary: Supported CHMP7 ESCRT-III-associated complex annotation: ESCRT III complex. Reason: CHMP7 is an ESCRT-III-like factor that recruits and associates with downstream ESCRT-III components, especially at the reforming nuclear envelope. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway file:human/CHMP7/CHMP7-notes.md CHMP7 is an ESCRT-III-like / ESCRT-II-III hybrid protein with two literature-supported contexts |
| GO:0009898 cytoplasmic side of plasma membrane | IBA GO_REF:0000033 | MARK AS OVER ANNOTATED | Summary: Plasma-membrane location/context is over-extended for CHMP7: cytoplasmic side of plasma membrane. Reason: CHMP7-specific evidence localizes its core recruitment function to the reforming nuclear envelope; plasma membrane context comes from broader ESCRT/viral-budding pathway inference. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Treat viral budding annotations as over-annotated for CHMP7. file:human/CHMP7/CHMP7-notes.md the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure |
| GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway | IBA GO_REF:0000033 | MODIFY | Summary: The endosomal-sorting evidence is real, but late endosome to vacuole transport via multivesicular body sorting pathway should be expressed with human MVB/endolysosomal terms. Reason: CHMP7 has an older supported endosomal-sorting phenotype, but vacuole wording is less appropriate for human curation than MVB sorting / late endosome-to-lysosome transport. Proposed replacements: multivesicular body sorting pathway late endosome to lysosome transport Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. |
| GO:0000776 kinetochore | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: kinetochore. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0001778 plasma membrane repair | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: plasma membrane repair. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0005635 nuclear envelope | IEA GO_REF:0000044 | ACCEPT | Summary: Supported core CHMP7 nuclear-envelope function: nuclear envelope. Reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for nuclear membrane closure/sealing. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery PMID:28242692 CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope UniProt:Q8WUX9 required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase file:human/CHMP7/CHMP7-notes.md the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure |
| GO:0005643 nuclear pore | IEA GO_REF:0000117 | MODIFY | Summary: The localization essence is nuclear-envelope recruitment, not stable membership in nuclear pore. Reason: CHMP7 localizes to reforming nuclear-envelope/chromatin-disk sites; nuclear envelope is the more accurate cellular-component term. Proposed replacements: nuclear envelope Supporting Evidence: PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope file:human/CHMP7/CHMP7-notes.md exit from mitosis, nuclear pore, chromatin location, and spindle-assembly wording should be interpreted through the specific CHMP7 role in nuclear membrane reassembly and nuclear-envelope sealing |
| GO:0005737 cytoplasm | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: cytoplasm. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0005765 lysosomal membrane | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: lysosomal membrane. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0005828 kinetochore microtubule | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: kinetochore microtubule. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0007034 vacuolar transport | IEA GO_REF:0000002 | MODIFY | Summary: The endosomal-sorting evidence is real, but vacuolar transport should be expressed with human MVB/endolysosomal terms. Reason: CHMP7 has an older supported endosomal-sorting phenotype, but vacuole wording is less appropriate for human curation than MVB sorting / late endosome-to-lysosome transport. Proposed replacements: multivesicular body sorting pathway late endosome to lysosome transport Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. |
| GO:0007080 mitotic metaphase chromosome alignment | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: mitotic metaphase chromosome alignment. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0030496 midbody | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: midbody. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0031468 nuclear membrane reassembly | IEA GO_REF:0000117 | ACCEPT | Summary: Supported core CHMP7 nuclear-envelope function: nuclear membrane reassembly. Reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for nuclear membrane closure/sealing. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery PMID:28242692 CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope UniProt:Q8WUX9 required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase file:human/CHMP7/CHMP7-notes.md the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure |
| GO:0032585 multivesicular body membrane | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Supported but secondary CHMP7 endosomal/MVB ESCRT context: multivesicular body membrane. Reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. |
| GO:0039702 viral budding via host ESCRT complex | IEA GO_REF:0000117 | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP7 viral-budding term: viral budding via host ESCRT complex. Reason: The CHMP7 viral evidence is a dominant-negative overexpression effect on virus-like particle release, not an endogenous core CHMP7 viral-budding function. Supporting Evidence: PMID:16856878 dominant-negative effect of overexpressed GFP-CHMP7 file:human/CHMP7/CHMP7-notes.md The direct evidence is dominant-negative overexpression affecting virus-like particle release, not a demonstrated endogenous core viral-budding function. |
| GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Supported but secondary CHMP7 endosomal/MVB ESCRT context: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway. Reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. |
| GO:0046761 viral budding from plasma membrane | IEA GO_REF:0000117 | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP7 viral-budding term: viral budding from plasma membrane. Reason: The CHMP7 viral evidence is a dominant-negative overexpression effect on virus-like particle release, not an endogenous core CHMP7 viral-budding function. Supporting Evidence: PMID:16856878 dominant-negative effect of overexpressed GFP-CHMP7 file:human/CHMP7/CHMP7-notes.md The direct evidence is dominant-negative overexpression affecting virus-like particle release, not a demonstrated endogenous core viral-budding function. |
| GO:0061952 midbody abscission | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: midbody abscission. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0071985 multivesicular body sorting pathway | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Supported but secondary CHMP7 endosomal/MVB ESCRT context: multivesicular body sorting pathway. Reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. |
| GO:0097352 autophagosome maturation | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: autophagosome maturation. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:1901673 regulation of mitotic spindle assembly | IEA GO_REF:0000117 | MODIFY | Summary: The cell-cycle/spindle wording is too broad for CHMP7: regulation of mitotic spindle assembly. Reason: CHMP7 contributes specifically to nuclear membrane reassembly and NE sealing during mitotic exit rather than to broad mitotic progression or spindle assembly. Proposed replacements: nuclear membrane reassembly Supporting Evidence: PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope file:human/CHMP7/CHMP7-notes.md exit from mitosis, nuclear pore, chromatin location, and spindle-assembly wording should be interpreted through the specific CHMP7 role in nuclear membrane reassembly and nuclear-envelope sealing |
| GO:1902774 late endosome to lysosome transport | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Supported but secondary CHMP7 endosomal/MVB ESCRT context: late endosome to lysosome transport. Reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. |
| GO:1904930 amphisome membrane | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: amphisome membrane. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0005515 protein binding | IPI PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CH... | MARK AS OVER ANNOTATED | Summary: Generic protein binding is over-annotated for CHMP7: protein binding. Reason: The meaningful interactions are CHMP7-LEMD2 and CHMP7-CHMP4B; GO:0005515 is too generic to represent CHMP7 function. Supporting Evidence: PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b PMID:28242692 CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro file:human/CHMP7/CHMP7-notes.md The meaningful molecular interactions are CHMP7-LEMD2 and CHMP7-CHMP4B, not undifferentiated protein binding. |
| GO:0005515 protein binding | IPI PMID:30194290 Interrogating the protein interactomes of RAS isoforms ident... | MARK AS OVER ANNOTATED | Summary: Generic protein binding is over-annotated for CHMP7: protein binding. Reason: The meaningful interactions are CHMP7-LEMD2 and CHMP7-CHMP4B; GO:0005515 is too generic to represent CHMP7 function. Supporting Evidence: PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b PMID:28242692 CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro file:human/CHMP7/CHMP7-notes.md The meaningful molecular interactions are CHMP7-LEMD2 and CHMP7-CHMP4B, not undifferentiated protein binding. |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | MARK AS OVER ANNOTATED | Summary: Generic protein binding is over-annotated for CHMP7: protein binding. Reason: The meaningful interactions are CHMP7-LEMD2 and CHMP7-CHMP4B; GO:0005515 is too generic to represent CHMP7 function. Supporting Evidence: PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b PMID:28242692 CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro file:human/CHMP7/CHMP7-notes.md The meaningful molecular interactions are CHMP7-LEMD2 and CHMP7-CHMP4B, not undifferentiated protein binding. |
| GO:0005654 nucleoplasm | IDA GO_REF:0000052 | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: nucleoplasm. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0005829 cytosol | IDA GO_REF:0000052 | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0000421 autophagosome membrane | IDA PMID:17984323 Functional multivesicular bodies are required for autophagic... | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: autophagosome membrane. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0000776 kinetochore | IDA PMID:26040712 Spastin and ESCRT-III coordinate mitotic spindle disassembly... | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: kinetochore. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0000815 ESCRT III complex | NAS PMID:36107470 Comprehensive analysis of the human ESCRT-III-MIT domain int... | ACCEPT | Summary: Supported CHMP7 ESCRT-III-associated complex annotation: ESCRT III complex. Reason: CHMP7 is an ESCRT-III-like factor that recruits and associates with downstream ESCRT-III components, especially at the reforming nuclear envelope. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway file:human/CHMP7/CHMP7-notes.md CHMP7 is an ESCRT-III-like / ESCRT-II-III hybrid protein with two literature-supported contexts |
| GO:0001778 plasma membrane repair | IDA PMID:24482116 ESCRT machinery is required for plasma membrane repair. | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: plasma membrane repair. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0005643 nuclear pore | IDA PMID:26040713 ESCRT-III controls nuclear envelope reformation. | MODIFY | Summary: The localization essence is nuclear-envelope recruitment, not stable membership in nuclear pore. Reason: CHMP7 localizes to reforming nuclear-envelope/chromatin-disk sites; nuclear envelope is the more accurate cellular-component term. Proposed replacements: nuclear envelope Supporting Evidence: PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope file:human/CHMP7/CHMP7-notes.md exit from mitosis, nuclear pore, chromatin location, and spindle-assembly wording should be interpreted through the specific CHMP7 role in nuclear membrane reassembly and nuclear-envelope sealing |
| GO:0005765 lysosomal membrane | IDA PMID:17984323 Functional multivesicular bodies are required for autophagic... | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: lysosomal membrane. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0005828 kinetochore microtubule | IDA PMID:26040712 Spastin and ESCRT-III coordinate mitotic spindle disassembly... | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: kinetochore microtubule. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0005886 plasma membrane | IDA PMID:24878737 Structure of cellular ESCRT-III spirals and their relationsh... | MARK AS OVER ANNOTATED | Summary: Plasma-membrane location/context is over-extended for CHMP7: plasma membrane. Reason: CHMP7-specific evidence localizes its core recruitment function to the reforming nuclear envelope; plasma membrane context comes from broader ESCRT/viral-budding pathway inference. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Treat viral budding annotations as over-annotated for CHMP7. file:human/CHMP7/CHMP7-notes.md the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure |
| GO:0006914 autophagy | IMP PMID:17984323 Functional multivesicular bodies are required for autophagic... | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: autophagy. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0006997 nucleus organization | IMP PMID:20616062 Human ESCRT-III and VPS4 proteins are required for centrosom... | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: nucleus organization. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0007080 mitotic metaphase chromosome alignment | IMP PMID:20616062 Human ESCRT-III and VPS4 proteins are required for centrosom... | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: mitotic metaphase chromosome alignment. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0030496 midbody | IDA PMID:26040712 Spastin and ESCRT-III coordinate mitotic spindle disassembly... | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: midbody. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0031468 nuclear membrane reassembly | IMP PMID:26040713 ESCRT-III controls nuclear envelope reformation. | ACCEPT | Summary: Supported core CHMP7 nuclear-envelope function: nuclear membrane reassembly. Reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for nuclear membrane closure/sealing. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery PMID:28242692 CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope UniProt:Q8WUX9 required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase file:human/CHMP7/CHMP7-notes.md the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure |
| GO:0032585 multivesicular body membrane | IDA PMID:16554368 The ESCRT-III subunit hVps24 is required for degradation but... | KEEP AS NON CORE | Summary: Supported but secondary CHMP7 endosomal/MVB ESCRT context: multivesicular body membrane. Reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. |
| GO:0036258 multivesicular body assembly | NAS PMID:16505166 Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a... | KEEP AS NON CORE | Summary: Supported but secondary CHMP7 endosomal/MVB ESCRT context: multivesicular body assembly. Reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. |
| GO:0039702 viral budding via host ESCRT complex | IDA PMID:24878737 Structure of cellular ESCRT-III spirals and their relationsh... | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP7 viral-budding term: viral budding via host ESCRT complex. Reason: The CHMP7 viral evidence is a dominant-negative overexpression effect on virus-like particle release, not an endogenous core CHMP7 viral-budding function. Supporting Evidence: PMID:16856878 dominant-negative effect of overexpressed GFP-CHMP7 file:human/CHMP7/CHMP7-notes.md The direct evidence is dominant-negative overexpression affecting virus-like particle release, not a demonstrated endogenous core viral-budding function. |
| GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway | IDA PMID:17984323 Functional multivesicular bodies are required for autophagic... | KEEP AS NON CORE | Summary: Supported but secondary CHMP7 endosomal/MVB ESCRT context: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway. Reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. |
| GO:0046761 viral budding from plasma membrane | IDA PMID:24878737 Structure of cellular ESCRT-III spirals and their relationsh... | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP7 viral-budding term: viral budding from plasma membrane. Reason: The CHMP7 viral evidence is a dominant-negative overexpression effect on virus-like particle release, not an endogenous core CHMP7 viral-budding function. Supporting Evidence: PMID:16856878 dominant-negative effect of overexpressed GFP-CHMP7 file:human/CHMP7/CHMP7-notes.md The direct evidence is dominant-negative overexpression affecting virus-like particle release, not a demonstrated endogenous core viral-budding function. |
| GO:0051469 vesicle fusion with vacuole | NAS PMID:16505166 Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a... | MODIFY | Summary: This fusion/vacuole annotation should be generalized to the better-supported endolysosomal route: vesicle fusion with vacuole. Reason: The available CHMP7 evidence supports endosomal sorting pathway involvement, not a specific vesicle-fusion activity. Proposed replacements: late endosome to lysosome transport Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. |
| GO:0061763 multivesicular body-lysosome fusion | NAS PMID:16505166 Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a... | MODIFY | Summary: This fusion/vacuole annotation should be generalized to the better-supported endolysosomal route: multivesicular body-lysosome fusion. Reason: The available CHMP7 evidence supports endosomal sorting pathway involvement, not a specific vesicle-fusion activity. Proposed replacements: late endosome to lysosome transport Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. |
| GO:0061952 midbody abscission | IMP PMID:20616062 Human ESCRT-III and VPS4 proteins are required for centrosom... | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: midbody abscission. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0071985 multivesicular body sorting pathway | IDA PMID:16554368 The ESCRT-III subunit hVps24 is required for degradation but... | KEEP AS NON CORE | Summary: Supported but secondary CHMP7 endosomal/MVB ESCRT context: multivesicular body sorting pathway. Reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. |
| GO:0090148 membrane fission | NAS PMID:19234443 Membrane scission by the ESCRT-III complex. | ACCEPT | Summary: Supported ESCRT membrane-remodeling output for CHMP7 nuclear-envelope closure: membrane fission. Reason: Nuclear-envelope sealing is an ESCRT membrane-closure/fission-like process and is the best-supported CHMP7-specific core function. Supporting Evidence: PMID:26040712 ESCRT-III, VPS4 and spastin cooperate to coordinate nuclear envelope sealing and spindle disassembly PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope file:human/CHMP7/CHMP7-notes.md Accept as core: nuclear envelope, nuclear membrane reassembly, ESCRT III complex, membrane fission |
| GO:0097352 autophagosome maturation | IMP PMID:17984323 Functional multivesicular bodies are required for autophagic... | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: autophagosome maturation. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:1901673 regulation of mitotic spindle assembly | IMP PMID:20616062 Human ESCRT-III and VPS4 proteins are required for centrosom... | MODIFY | Summary: The cell-cycle/spindle wording is too broad for CHMP7: regulation of mitotic spindle assembly. Reason: CHMP7 contributes specifically to nuclear membrane reassembly and NE sealing during mitotic exit rather than to broad mitotic progression or spindle assembly. Proposed replacements: nuclear membrane reassembly Supporting Evidence: PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope file:human/CHMP7/CHMP7-notes.md exit from mitosis, nuclear pore, chromatin location, and spindle-assembly wording should be interpreted through the specific CHMP7 role in nuclear membrane reassembly and nuclear-envelope sealing |
| GO:1902774 late endosome to lysosome transport | IMP PMID:17984323 Functional multivesicular bodies are required for autophagic... | KEEP AS NON CORE | Summary: Supported but secondary CHMP7 endosomal/MVB ESCRT context: late endosome to lysosome transport. Reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. |
| GO:1904930 amphisome membrane | IDA PMID:17984323 Functional multivesicular bodies are required for autophagic... | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: amphisome membrane. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0005635 nuclear envelope | EXP PMID:28242692 LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope clos... | ACCEPT | Summary: Supported core CHMP7 nuclear-envelope function: nuclear envelope. Reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for nuclear membrane closure/sealing. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery PMID:28242692 CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope UniProt:Q8WUX9 required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase file:human/CHMP7/CHMP7-notes.md the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure |
| GO:0005737 cytoplasm | EXP PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CH... | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: cytoplasm. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0000785 chromatin | IDA PMID:28242692 LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope clos... | MODIFY | Summary: The localization essence is nuclear-envelope recruitment, not stable membership in chromatin. Reason: CHMP7 localizes to reforming nuclear-envelope/chromatin-disk sites; nuclear envelope is the more accurate cellular-component term. Proposed replacements: nuclear envelope Supporting Evidence: PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope file:human/CHMP7/CHMP7-notes.md exit from mitosis, nuclear pore, chromatin location, and spindle-assembly wording should be interpreted through the specific CHMP7 role in nuclear membrane reassembly and nuclear-envelope sealing |
| GO:0031468 nuclear membrane reassembly | TAS PMID:28242692 LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope clos... | ACCEPT | Summary: Supported core CHMP7 nuclear-envelope function: nuclear membrane reassembly. Reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for nuclear membrane closure/sealing. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery PMID:28242692 CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope UniProt:Q8WUX9 required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase file:human/CHMP7/CHMP7-notes.md the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure |
| GO:0071168 protein localization to chromatin | IMP PMID:28242692 LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope clos... | ACCEPT | Summary: Supported CHMP7-dependent recruitment of ESCRT factors to chromatin disks/reforming NE: protein localization to chromatin. Reason: CHMP7-dependent recruitment of IST1/CHMP8 and CHMP2A to chromatin disks is part of its nuclear-envelope ESCRT recruitment function. Supporting Evidence: PMID:28242692 CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope file:human/CHMP7/CHMP7-notes.md protein localization to chromatin / chromatin disk periphery where this represents LEMD2-dependent recruitment of CHMP7 and downstream ESCRT factors to the reforming nuclear envelope |
| GO:0000815 ESCRT III complex | NAS PMID:28242692 LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope clos... | ACCEPT | Summary: Supported CHMP7 ESCRT-III-associated complex annotation: ESCRT III complex. Reason: CHMP7 is an ESCRT-III-like factor that recruits and associates with downstream ESCRT-III components, especially at the reforming nuclear envelope. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway file:human/CHMP7/CHMP7-notes.md CHMP7 is an ESCRT-III-like / ESCRT-II-III hybrid protein with two literature-supported contexts |
| GO:0005515 protein binding | IPI PMID:28242692 LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope clos... | MARK AS OVER ANNOTATED | Summary: Generic protein binding is over-annotated for CHMP7: protein binding. Reason: The meaningful interactions are CHMP7-LEMD2 and CHMP7-CHMP4B; GO:0005515 is too generic to represent CHMP7 function. Supporting Evidence: PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b PMID:28242692 CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro file:human/CHMP7/CHMP7-notes.md The meaningful molecular interactions are CHMP7-LEMD2 and CHMP7-CHMP4B, not undifferentiated protein binding. |
| GO:0000815 ESCRT III complex | IDA PMID:26040712 Spastin and ESCRT-III coordinate mitotic spindle disassembly... | ACCEPT | Summary: Supported CHMP7 ESCRT-III-associated complex annotation: ESCRT III complex. Reason: CHMP7 is an ESCRT-III-like factor that recruits and associates with downstream ESCRT-III components, especially at the reforming nuclear envelope. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway file:human/CHMP7/CHMP7-notes.md CHMP7 is an ESCRT-III-like / ESCRT-II-III hybrid protein with two literature-supported contexts |
| GO:0005635 nuclear envelope | IDA PMID:26040712 Spastin and ESCRT-III coordinate mitotic spindle disassembly... | ACCEPT | Summary: Supported core CHMP7 nuclear-envelope function: nuclear envelope. Reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for nuclear membrane closure/sealing. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery PMID:28242692 CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope UniProt:Q8WUX9 required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase file:human/CHMP7/CHMP7-notes.md the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure |
| GO:0010458 exit from mitosis | IMP PMID:26040712 Spastin and ESCRT-III coordinate mitotic spindle disassembly... | MODIFY | Summary: The cell-cycle/spindle wording is too broad for CHMP7: exit from mitosis. Reason: CHMP7 contributes specifically to nuclear membrane reassembly and NE sealing during mitotic exit rather than to broad mitotic progression or spindle assembly. Proposed replacements: nuclear membrane reassembly Supporting Evidence: PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope file:human/CHMP7/CHMP7-notes.md exit from mitosis, nuclear pore, chromatin location, and spindle-assembly wording should be interpreted through the specific CHMP7 role in nuclear membrane reassembly and nuclear-envelope sealing |
| GO:0031468 nuclear membrane reassembly | IMP PMID:26040712 Spastin and ESCRT-III coordinate mitotic spindle disassembly... | ACCEPT | Summary: Supported core CHMP7 nuclear-envelope function: nuclear membrane reassembly. Reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for nuclear membrane closure/sealing. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:26040712 coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites PMID:28242692 CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery PMID:28242692 CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope UniProt:Q8WUX9 required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase file:human/CHMP7/CHMP7-notes.md the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure |
| GO:0036258 multivesicular body assembly | NAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a... | KEEP AS NON CORE | Summary: Supported but secondary CHMP7 endosomal/MVB ESCRT context: multivesicular body assembly. Reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. |
| GO:0039702 viral budding via host ESCRT complex | NAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a... | MARK AS OVER ANNOTATED | Summary: Over-annotated CHMP7 viral-budding term: viral budding via host ESCRT complex. Reason: The CHMP7 viral evidence is a dominant-negative overexpression effect on virus-like particle release, not an endogenous core CHMP7 viral-budding function. Supporting Evidence: PMID:16856878 dominant-negative effect of overexpressed GFP-CHMP7 file:human/CHMP7/CHMP7-notes.md The direct evidence is dominant-negative overexpression affecting virus-like particle release, not a demonstrated endogenous core viral-budding function. |
| GO:1904903 ESCRT III complex disassembly | NAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a... | KEEP AS NON CORE | Summary: Supported but secondary CHMP7 endosomal/MVB ESCRT context: ESCRT III complex disassembly. Reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-3159232 | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0005829 cytosol | TAS Reactome:R-HSA-917693 | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0005829 cytosol | TAS Reactome:R-HSA-917700 | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9668335 | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9668389 | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9668395 | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9668398 | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9668405 | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9668415 | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9668419 | KEEP AS NON CORE | Summary: Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol. Reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. Supporting Evidence: file:human/CHMP7/CHMP7-notes.md Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts |
| GO:0000815 ESCRT III complex | IDA PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CH... | ACCEPT | Summary: Supported CHMP7 ESCRT-III-associated complex annotation: ESCRT III complex. Reason: CHMP7 is an ESCRT-III-like factor that recruits and associates with downstream ESCRT-III components, especially at the reforming nuclear envelope. Supporting Evidence: PMID:26040712 ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules PMID:28242692 recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway file:human/CHMP7/CHMP7-notes.md CHMP7 is an ESCRT-III-like / ESCRT-II-III hybrid protein with two literature-supported contexts |
| GO:0045324 late endosome to vacuole transport | IMP PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CH... | MODIFY | Summary: The endosomal-sorting evidence is real, but late endosome to vacuole transport should be expressed with human MVB/endolysosomal terms. Reason: CHMP7 has an older supported endosomal-sorting phenotype, but vacuole wording is less appropriate for human curation than MVB sorting / late endosome-to-lysosome transport. Proposed replacements: multivesicular body sorting pathway late endosome to lysosome transport Supporting Evidence: PMID:16856878 CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway PMID:16856878 positive interaction between the C-terminal half of CHMP7 and CHMP4b file:human/CHMP7/CHMP7-notes.md PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. |
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Download this section (compressed HTML)Q: Can endogenous CHMP7 rescue assays separate LEMD2 binding, CHMP4B recruitment, and VPS4-dependent ESCRT turnover during nuclear-envelope sealing?
Q: Should CHMP7 endosomal-sorting annotations remain non-core unless supported by endogenous depletion/rescue assays distinct from dominant-negative overexpression phenotypes?
Q: Is there a more specific GO process term needed for ESCRT-mediated nuclear-envelope hole sealing during anaphase?
Experiment: Rescue CHMP7-depleted cells with wild-type CHMP7 and mutants separating LEMD2 binding, CHMP4B binding, and ESCRT-III recruitment while measuring CHMP2A/IST1 recruitment, NE sealing reporters, and post-mitotic nuclear leakage.
Hypothesis: CHMP7 nuclear-envelope sealing will require LEMD2-dependent recruitment and downstream ESCRT-III assembly more directly than its endosomal-sorting phenotype.
Experiment: Compare acute endogenous CHMP7 depletion/rescue in EGFR or ubiquitinated-cargo MVB sorting assays versus nuclear-envelope sealing assays under matched expression levels.
Hypothesis: The nuclear-envelope phenotype will be the clearest CHMP7-specific core function, whereas endosomal sorting will be weaker or context-dependent.
Experiment: Test whether CHMP7 perturbation affects virus-like particle release only through overexpression/dominant-negative ESCRT disruption or through a reproducible endogenous requirement.
Hypothesis: Viral-budding effects will reflect pathway-level ESCRT perturbation rather than a direct core CHMP7 function.
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