id: Q8WUX9 gene_symbol: CHMP7 product_type: PROTEIN status: COMPLETE taxon: id: NCBITaxon:9606 label: Homo sapiens description: >- CHMP7 encodes an ESCRT-III-like / ESCRT-II-III hybrid protein that acts most specifically as an upstream adaptor for nuclear-envelope ESCRT recruitment. During late anaphase, LEMD2/LEM2 recruits CHMP7 to the reforming nuclear envelope and chromatin-disk periphery, where CHMP7 helps recruit downstream ESCRT-III components such as CHMP2A and IST1/CHMP8 to seal nuclear-envelope holes and coordinate spindle microtubule disassembly. CHMP7 also has evidence for CHMP4B-associated endosomal sorting, but this appears secondary to its better-defined nuclear-envelope repair/sealing role. alternative_products: - name: '1' id: Q8WUX9-1 - name: '2' id: Q8WUX9-2 sequence_note: VSP_056945, VSP_056946 existing_annotations: - term: id: GO:0005635 label: nuclear envelope evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: is_active_in review: summary: 'Supported core CHMP7 nuclear-envelope function: nuclear envelope.' action: ACCEPT reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for nuclear membrane closure/sealing. supported_by: - reference_id: PMID:26040712 supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules - reference_id: PMID:26040712 supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites - reference_id: PMID:28242692 supporting_text: CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery - reference_id: PMID:28242692 supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro - reference_id: PMID:28242692 supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope - reference_id: UniProt:Q8WUX9 supporting_text: required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure - term: id: GO:0031468 label: nuclear membrane reassembly evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: involved_in review: summary: 'Supported core CHMP7 nuclear-envelope function: nuclear membrane reassembly.' action: ACCEPT reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for nuclear membrane closure/sealing. supported_by: - reference_id: PMID:26040712 supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules - reference_id: PMID:26040712 supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites - reference_id: PMID:28242692 supporting_text: CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery - reference_id: PMID:28242692 supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro - reference_id: PMID:28242692 supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope - reference_id: UniProt:Q8WUX9 supporting_text: required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure - term: id: GO:0000815 label: ESCRT III complex evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: part_of review: summary: 'Supported CHMP7 ESCRT-III-associated complex annotation: ESCRT III complex.' action: ACCEPT reason: CHMP7 is an ESCRT-III-like factor that recruits and associates with downstream ESCRT-III components, especially at the reforming nuclear envelope. supported_by: - reference_id: PMID:26040712 supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules - reference_id: PMID:28242692 supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope - reference_id: PMID:16856878 supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: CHMP7 is an ESCRT-III-like / ESCRT-II-III hybrid protein with two literature-supported contexts - term: id: GO:0009898 label: cytoplasmic side of plasma membrane evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: is_active_in review: summary: 'Plasma-membrane location/context is over-extended for CHMP7: cytoplasmic side of plasma membrane.' action: MARK_AS_OVER_ANNOTATED reason: CHMP7-specific evidence localizes its core recruitment function to the reforming nuclear envelope; plasma membrane context comes from broader ESCRT/viral-budding pathway inference. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Treat viral budding annotations as over-annotated for CHMP7. - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure - term: id: GO:0032511 label: late endosome to vacuole transport via multivesicular body sorting pathway evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: involved_in review: summary: The endosomal-sorting evidence is real, but late endosome to vacuole transport via multivesicular body sorting pathway should be expressed with human MVB/endolysosomal terms. action: MODIFY reason: CHMP7 has an older supported endosomal-sorting phenotype, but vacuole wording is less appropriate for human curation than MVB sorting / late endosome-to-lysosome transport. supported_by: - reference_id: PMID:16856878 supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway - reference_id: PMID:16856878 supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. proposed_replacement_terms: - id: GO:0071985 label: multivesicular body sorting pathway - id: GO:1902774 label: late endosome to lysosome transport - term: id: GO:0000776 label: kinetochore evidence_type: IEA original_reference_id: GO_REF:0000117 qualifier: located_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: kinetochore.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0001778 label: plasma membrane repair evidence_type: IEA original_reference_id: GO_REF:0000117 qualifier: involved_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: plasma membrane repair.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0005635 label: nuclear envelope evidence_type: IEA original_reference_id: GO_REF:0000044 qualifier: located_in review: summary: 'Supported core CHMP7 nuclear-envelope function: nuclear envelope.' action: ACCEPT reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for nuclear membrane closure/sealing. supported_by: - reference_id: PMID:26040712 supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules - reference_id: PMID:26040712 supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites - reference_id: PMID:28242692 supporting_text: CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery - reference_id: PMID:28242692 supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro - reference_id: PMID:28242692 supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope - reference_id: UniProt:Q8WUX9 supporting_text: required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure - term: id: GO:0005643 label: nuclear pore evidence_type: IEA original_reference_id: GO_REF:0000117 qualifier: part_of review: summary: The localization essence is nuclear-envelope recruitment, not stable membership in nuclear pore. action: MODIFY reason: CHMP7 localizes to reforming nuclear-envelope/chromatin-disk sites; nuclear envelope is the more accurate cellular-component term. supported_by: - reference_id: PMID:26040712 supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites - reference_id: PMID:28242692 supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: exit from mitosis, nuclear pore, chromatin location, and spindle-assembly wording should be interpreted through the specific CHMP7 role in nuclear membrane reassembly and nuclear-envelope sealing proposed_replacement_terms: - id: GO:0005635 label: nuclear envelope - term: id: GO:0005737 label: cytoplasm evidence_type: IEA original_reference_id: GO_REF:0000044 qualifier: located_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: cytoplasm.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0005765 label: lysosomal membrane evidence_type: IEA original_reference_id: GO_REF:0000117 qualifier: located_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: lysosomal membrane.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0005828 label: kinetochore microtubule evidence_type: IEA original_reference_id: GO_REF:0000117 qualifier: located_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: kinetochore microtubule.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0007034 label: vacuolar transport evidence_type: IEA original_reference_id: GO_REF:0000002 qualifier: involved_in review: summary: The endosomal-sorting evidence is real, but vacuolar transport should be expressed with human MVB/endolysosomal terms. action: MODIFY reason: CHMP7 has an older supported endosomal-sorting phenotype, but vacuole wording is less appropriate for human curation than MVB sorting / late endosome-to-lysosome transport. supported_by: - reference_id: PMID:16856878 supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway - reference_id: PMID:16856878 supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. proposed_replacement_terms: - id: GO:0071985 label: multivesicular body sorting pathway - id: GO:1902774 label: late endosome to lysosome transport - term: id: GO:0007080 label: mitotic metaphase chromosome alignment evidence_type: IEA original_reference_id: GO_REF:0000117 qualifier: involved_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: mitotic metaphase chromosome alignment.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0030496 label: midbody evidence_type: IEA original_reference_id: GO_REF:0000117 qualifier: located_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: midbody.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0031468 label: nuclear membrane reassembly evidence_type: IEA original_reference_id: GO_REF:0000117 qualifier: involved_in review: summary: 'Supported core CHMP7 nuclear-envelope function: nuclear membrane reassembly.' action: ACCEPT reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for nuclear membrane closure/sealing. supported_by: - reference_id: PMID:26040712 supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules - reference_id: PMID:26040712 supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites - reference_id: PMID:28242692 supporting_text: CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery - reference_id: PMID:28242692 supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro - reference_id: PMID:28242692 supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope - reference_id: UniProt:Q8WUX9 supporting_text: required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure - term: id: GO:0032585 label: multivesicular body membrane evidence_type: IEA original_reference_id: GO_REF:0000117 qualifier: located_in review: summary: 'Supported but secondary CHMP7 endosomal/MVB ESCRT context: multivesicular body membrane.' action: KEEP_AS_NON_CORE reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. supported_by: - reference_id: PMID:16856878 supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway - reference_id: PMID:16856878 supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. - term: id: GO:0039702 label: viral budding via host ESCRT complex evidence_type: IEA original_reference_id: GO_REF:0000117 qualifier: involved_in review: summary: 'Over-annotated CHMP7 viral-budding term: viral budding via host ESCRT complex.' action: MARK_AS_OVER_ANNOTATED reason: The CHMP7 viral evidence is a dominant-negative overexpression effect on virus-like particle release, not an endogenous core CHMP7 viral-budding function. supported_by: - reference_id: PMID:16856878 supporting_text: dominant-negative effect of overexpressed GFP-CHMP7 - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: The direct evidence is dominant-negative overexpression affecting virus-like particle release, not a demonstrated endogenous core viral-budding function. - term: id: GO:0043162 label: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway evidence_type: IEA original_reference_id: GO_REF:0000117 qualifier: involved_in review: summary: 'Supported but secondary CHMP7 endosomal/MVB ESCRT context: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway.' action: KEEP_AS_NON_CORE reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. supported_by: - reference_id: PMID:16856878 supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway - reference_id: PMID:16856878 supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. - term: id: GO:0046761 label: viral budding from plasma membrane evidence_type: IEA original_reference_id: GO_REF:0000117 qualifier: involved_in review: summary: 'Over-annotated CHMP7 viral-budding term: viral budding from plasma membrane.' action: MARK_AS_OVER_ANNOTATED reason: The CHMP7 viral evidence is a dominant-negative overexpression effect on virus-like particle release, not an endogenous core CHMP7 viral-budding function. supported_by: - reference_id: PMID:16856878 supporting_text: dominant-negative effect of overexpressed GFP-CHMP7 - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: The direct evidence is dominant-negative overexpression affecting virus-like particle release, not a demonstrated endogenous core viral-budding function. - term: id: GO:0061952 label: midbody abscission evidence_type: IEA original_reference_id: GO_REF:0000117 qualifier: involved_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: midbody abscission.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0071985 label: multivesicular body sorting pathway evidence_type: IEA original_reference_id: GO_REF:0000117 qualifier: involved_in review: summary: 'Supported but secondary CHMP7 endosomal/MVB ESCRT context: multivesicular body sorting pathway.' action: KEEP_AS_NON_CORE reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. supported_by: - reference_id: PMID:16856878 supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway - reference_id: PMID:16856878 supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. - term: id: GO:0097352 label: autophagosome maturation evidence_type: IEA original_reference_id: GO_REF:0000117 qualifier: involved_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: autophagosome maturation.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:1901673 label: regulation of mitotic spindle assembly evidence_type: IEA original_reference_id: GO_REF:0000117 qualifier: involved_in review: summary: 'The cell-cycle/spindle wording is too broad for CHMP7: regulation of mitotic spindle assembly.' action: MODIFY reason: CHMP7 contributes specifically to nuclear membrane reassembly and NE sealing during mitotic exit rather than to broad mitotic progression or spindle assembly. supported_by: - reference_id: PMID:26040712 supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites - reference_id: PMID:28242692 supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: exit from mitosis, nuclear pore, chromatin location, and spindle-assembly wording should be interpreted through the specific CHMP7 role in nuclear membrane reassembly and nuclear-envelope sealing proposed_replacement_terms: - id: GO:0031468 label: nuclear membrane reassembly - term: id: GO:1902774 label: late endosome to lysosome transport evidence_type: IEA original_reference_id: GO_REF:0000117 qualifier: involved_in review: summary: 'Supported but secondary CHMP7 endosomal/MVB ESCRT context: late endosome to lysosome transport.' action: KEEP_AS_NON_CORE reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. supported_by: - reference_id: PMID:16856878 supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway - reference_id: PMID:16856878 supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. - term: id: GO:1904930 label: amphisome membrane evidence_type: IEA original_reference_id: GO_REF:0000117 qualifier: located_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: amphisome membrane.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:16856878 qualifier: enables review: summary: 'Generic protein binding is over-annotated for CHMP7: protein binding.' action: MARK_AS_OVER_ANNOTATED reason: The meaningful interactions are CHMP7-LEMD2 and CHMP7-CHMP4B; GO:0005515 is too generic to represent CHMP7 function. supported_by: - reference_id: PMID:16856878 supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b - reference_id: PMID:28242692 supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: The meaningful molecular interactions are CHMP7-LEMD2 and CHMP7-CHMP4B, not undifferentiated protein binding. - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:30194290 qualifier: enables review: summary: 'Generic protein binding is over-annotated for CHMP7: protein binding.' action: MARK_AS_OVER_ANNOTATED reason: The meaningful interactions are CHMP7-LEMD2 and CHMP7-CHMP4B; GO:0005515 is too generic to represent CHMP7 function. supported_by: - reference_id: PMID:16856878 supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b - reference_id: PMID:28242692 supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: The meaningful molecular interactions are CHMP7-LEMD2 and CHMP7-CHMP4B, not undifferentiated protein binding. - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:32814053 qualifier: enables review: summary: 'Generic protein binding is over-annotated for CHMP7: protein binding.' action: MARK_AS_OVER_ANNOTATED reason: The meaningful interactions are CHMP7-LEMD2 and CHMP7-CHMP4B; GO:0005515 is too generic to represent CHMP7 function. supported_by: - reference_id: PMID:16856878 supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b - reference_id: PMID:28242692 supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: The meaningful molecular interactions are CHMP7-LEMD2 and CHMP7-CHMP4B, not undifferentiated protein binding. - term: id: GO:0005654 label: nucleoplasm evidence_type: IDA original_reference_id: GO_REF:0000052 qualifier: located_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: nucleoplasm.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0005829 label: cytosol evidence_type: IDA original_reference_id: GO_REF:0000052 qualifier: located_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0000421 label: autophagosome membrane evidence_type: IDA original_reference_id: PMID:17984323 qualifier: located_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: autophagosome membrane.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0000776 label: kinetochore evidence_type: IDA original_reference_id: PMID:26040712 qualifier: located_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: kinetochore.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0000815 label: ESCRT III complex evidence_type: NAS original_reference_id: PMID:36107470 qualifier: part_of review: summary: 'Supported CHMP7 ESCRT-III-associated complex annotation: ESCRT III complex.' action: ACCEPT reason: CHMP7 is an ESCRT-III-like factor that recruits and associates with downstream ESCRT-III components, especially at the reforming nuclear envelope. supported_by: - reference_id: PMID:26040712 supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules - reference_id: PMID:28242692 supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope - reference_id: PMID:16856878 supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: CHMP7 is an ESCRT-III-like / ESCRT-II-III hybrid protein with two literature-supported contexts - term: id: GO:0001778 label: plasma membrane repair evidence_type: IDA original_reference_id: PMID:24482116 qualifier: involved_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: plasma membrane repair.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0005643 label: nuclear pore evidence_type: IDA original_reference_id: PMID:26040713 qualifier: part_of review: summary: The localization essence is nuclear-envelope recruitment, not stable membership in nuclear pore. action: MODIFY reason: CHMP7 localizes to reforming nuclear-envelope/chromatin-disk sites; nuclear envelope is the more accurate cellular-component term. supported_by: - reference_id: PMID:26040712 supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites - reference_id: PMID:28242692 supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: exit from mitosis, nuclear pore, chromatin location, and spindle-assembly wording should be interpreted through the specific CHMP7 role in nuclear membrane reassembly and nuclear-envelope sealing proposed_replacement_terms: - id: GO:0005635 label: nuclear envelope - term: id: GO:0005765 label: lysosomal membrane evidence_type: IDA original_reference_id: PMID:17984323 qualifier: located_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: lysosomal membrane.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0005828 label: kinetochore microtubule evidence_type: IDA original_reference_id: PMID:26040712 qualifier: located_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: kinetochore microtubule.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0005886 label: plasma membrane evidence_type: IDA original_reference_id: PMID:24878737 qualifier: located_in review: summary: 'Plasma-membrane location/context is over-extended for CHMP7: plasma membrane.' action: MARK_AS_OVER_ANNOTATED reason: CHMP7-specific evidence localizes its core recruitment function to the reforming nuclear envelope; plasma membrane context comes from broader ESCRT/viral-budding pathway inference. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Treat viral budding annotations as over-annotated for CHMP7. - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure - term: id: GO:0006914 label: autophagy evidence_type: IMP original_reference_id: PMID:17984323 qualifier: involved_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: autophagy.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0006997 label: nucleus organization evidence_type: IMP original_reference_id: PMID:20616062 qualifier: involved_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: nucleus organization.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0007080 label: mitotic metaphase chromosome alignment evidence_type: IMP original_reference_id: PMID:20616062 qualifier: involved_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: mitotic metaphase chromosome alignment.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0030496 label: midbody evidence_type: IDA original_reference_id: PMID:26040712 qualifier: located_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: midbody.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0031468 label: nuclear membrane reassembly evidence_type: IMP original_reference_id: PMID:26040713 qualifier: involved_in review: summary: 'Supported core CHMP7 nuclear-envelope function: nuclear membrane reassembly.' action: ACCEPT reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for nuclear membrane closure/sealing. supported_by: - reference_id: PMID:26040712 supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules - reference_id: PMID:26040712 supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites - reference_id: PMID:28242692 supporting_text: CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery - reference_id: PMID:28242692 supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro - reference_id: PMID:28242692 supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope - reference_id: UniProt:Q8WUX9 supporting_text: required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure - term: id: GO:0032585 label: multivesicular body membrane evidence_type: IDA original_reference_id: PMID:16554368 qualifier: located_in review: summary: 'Supported but secondary CHMP7 endosomal/MVB ESCRT context: multivesicular body membrane.' action: KEEP_AS_NON_CORE reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. supported_by: - reference_id: PMID:16856878 supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway - reference_id: PMID:16856878 supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. - term: id: GO:0036258 label: multivesicular body assembly evidence_type: NAS original_reference_id: PMID:16505166 qualifier: involved_in review: summary: 'Supported but secondary CHMP7 endosomal/MVB ESCRT context: multivesicular body assembly.' action: KEEP_AS_NON_CORE reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. supported_by: - reference_id: PMID:16856878 supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway - reference_id: PMID:16856878 supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. - term: id: GO:0039702 label: viral budding via host ESCRT complex evidence_type: IDA original_reference_id: PMID:24878737 qualifier: involved_in review: summary: 'Over-annotated CHMP7 viral-budding term: viral budding via host ESCRT complex.' action: MARK_AS_OVER_ANNOTATED reason: The CHMP7 viral evidence is a dominant-negative overexpression effect on virus-like particle release, not an endogenous core CHMP7 viral-budding function. supported_by: - reference_id: PMID:16856878 supporting_text: dominant-negative effect of overexpressed GFP-CHMP7 - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: The direct evidence is dominant-negative overexpression affecting virus-like particle release, not a demonstrated endogenous core viral-budding function. - term: id: GO:0043162 label: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway evidence_type: IDA original_reference_id: PMID:17984323 qualifier: involved_in review: summary: 'Supported but secondary CHMP7 endosomal/MVB ESCRT context: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway.' action: KEEP_AS_NON_CORE reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. supported_by: - reference_id: PMID:16856878 supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway - reference_id: PMID:16856878 supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. - term: id: GO:0046761 label: viral budding from plasma membrane evidence_type: IDA original_reference_id: PMID:24878737 qualifier: involved_in review: summary: 'Over-annotated CHMP7 viral-budding term: viral budding from plasma membrane.' action: MARK_AS_OVER_ANNOTATED reason: The CHMP7 viral evidence is a dominant-negative overexpression effect on virus-like particle release, not an endogenous core CHMP7 viral-budding function. supported_by: - reference_id: PMID:16856878 supporting_text: dominant-negative effect of overexpressed GFP-CHMP7 - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: The direct evidence is dominant-negative overexpression affecting virus-like particle release, not a demonstrated endogenous core viral-budding function. - term: id: GO:0051469 label: vesicle fusion with vacuole evidence_type: NAS original_reference_id: PMID:16505166 qualifier: involved_in review: summary: 'This fusion/vacuole annotation should be generalized to the better-supported endolysosomal route: vesicle fusion with vacuole.' action: MODIFY reason: The available CHMP7 evidence supports endosomal sorting pathway involvement, not a specific vesicle-fusion activity. supported_by: - reference_id: PMID:16856878 supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway - reference_id: PMID:16856878 supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. proposed_replacement_terms: - id: GO:1902774 label: late endosome to lysosome transport - term: id: GO:0061763 label: multivesicular body-lysosome fusion evidence_type: NAS original_reference_id: PMID:16505166 qualifier: involved_in review: summary: 'This fusion/vacuole annotation should be generalized to the better-supported endolysosomal route: multivesicular body-lysosome fusion.' action: MODIFY reason: The available CHMP7 evidence supports endosomal sorting pathway involvement, not a specific vesicle-fusion activity. supported_by: - reference_id: PMID:16856878 supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway - reference_id: PMID:16856878 supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. proposed_replacement_terms: - id: GO:1902774 label: late endosome to lysosome transport - term: id: GO:0061952 label: midbody abscission evidence_type: IMP original_reference_id: PMID:20616062 qualifier: involved_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: midbody abscission.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0071985 label: multivesicular body sorting pathway evidence_type: IDA original_reference_id: PMID:16554368 qualifier: involved_in review: summary: 'Supported but secondary CHMP7 endosomal/MVB ESCRT context: multivesicular body sorting pathway.' action: KEEP_AS_NON_CORE reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. supported_by: - reference_id: PMID:16856878 supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway - reference_id: PMID:16856878 supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. - term: id: GO:0090148 label: membrane fission evidence_type: NAS original_reference_id: PMID:19234443 qualifier: involved_in review: summary: 'Supported ESCRT membrane-remodeling output for CHMP7 nuclear-envelope closure: membrane fission.' action: ACCEPT reason: Nuclear-envelope sealing is an ESCRT membrane-closure/fission-like process and is the best-supported CHMP7-specific core function. supported_by: - reference_id: PMID:26040712 supporting_text: ESCRT-III, VPS4 and spastin cooperate to coordinate nuclear envelope sealing and spindle disassembly - reference_id: PMID:28242692 supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: 'Accept as core: nuclear envelope, nuclear membrane reassembly, ESCRT III complex, membrane fission' - term: id: GO:0097352 label: autophagosome maturation evidence_type: IMP original_reference_id: PMID:17984323 qualifier: involved_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: autophagosome maturation.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:1901673 label: regulation of mitotic spindle assembly evidence_type: IMP original_reference_id: PMID:20616062 qualifier: involved_in review: summary: 'The cell-cycle/spindle wording is too broad for CHMP7: regulation of mitotic spindle assembly.' action: MODIFY reason: CHMP7 contributes specifically to nuclear membrane reassembly and NE sealing during mitotic exit rather than to broad mitotic progression or spindle assembly. supported_by: - reference_id: PMID:26040712 supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites - reference_id: PMID:28242692 supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: exit from mitosis, nuclear pore, chromatin location, and spindle-assembly wording should be interpreted through the specific CHMP7 role in nuclear membrane reassembly and nuclear-envelope sealing proposed_replacement_terms: - id: GO:0031468 label: nuclear membrane reassembly - term: id: GO:1902774 label: late endosome to lysosome transport evidence_type: IMP original_reference_id: PMID:17984323 qualifier: involved_in review: summary: 'Supported but secondary CHMP7 endosomal/MVB ESCRT context: late endosome to lysosome transport.' action: KEEP_AS_NON_CORE reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. supported_by: - reference_id: PMID:16856878 supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway - reference_id: PMID:16856878 supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. - term: id: GO:1904930 label: amphisome membrane evidence_type: IDA original_reference_id: PMID:17984323 qualifier: located_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: amphisome membrane.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0005635 label: nuclear envelope evidence_type: EXP original_reference_id: PMID:28242692 qualifier: located_in review: summary: 'Supported core CHMP7 nuclear-envelope function: nuclear envelope.' action: ACCEPT reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for nuclear membrane closure/sealing. supported_by: - reference_id: PMID:26040712 supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules - reference_id: PMID:26040712 supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites - reference_id: PMID:28242692 supporting_text: CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery - reference_id: PMID:28242692 supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro - reference_id: PMID:28242692 supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope - reference_id: UniProt:Q8WUX9 supporting_text: required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure - term: id: GO:0005737 label: cytoplasm evidence_type: EXP original_reference_id: PMID:16856878 qualifier: located_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: cytoplasm.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0000785 label: chromatin evidence_type: IDA original_reference_id: PMID:28242692 qualifier: located_in review: summary: The localization essence is nuclear-envelope recruitment, not stable membership in chromatin. action: MODIFY reason: CHMP7 localizes to reforming nuclear-envelope/chromatin-disk sites; nuclear envelope is the more accurate cellular-component term. supported_by: - reference_id: PMID:26040712 supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites - reference_id: PMID:28242692 supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: exit from mitosis, nuclear pore, chromatin location, and spindle-assembly wording should be interpreted through the specific CHMP7 role in nuclear membrane reassembly and nuclear-envelope sealing proposed_replacement_terms: - id: GO:0005635 label: nuclear envelope - term: id: GO:0031468 label: nuclear membrane reassembly evidence_type: TAS original_reference_id: PMID:28242692 qualifier: involved_in review: summary: 'Supported core CHMP7 nuclear-envelope function: nuclear membrane reassembly.' action: ACCEPT reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for nuclear membrane closure/sealing. supported_by: - reference_id: PMID:26040712 supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules - reference_id: PMID:26040712 supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites - reference_id: PMID:28242692 supporting_text: CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery - reference_id: PMID:28242692 supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro - reference_id: PMID:28242692 supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope - reference_id: UniProt:Q8WUX9 supporting_text: required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure - term: id: GO:0071168 label: protein localization to chromatin evidence_type: IMP original_reference_id: PMID:28242692 qualifier: involved_in review: summary: 'Supported CHMP7-dependent recruitment of ESCRT factors to chromatin disks/reforming NE: protein localization to chromatin.' action: ACCEPT reason: CHMP7-dependent recruitment of IST1/CHMP8 and CHMP2A to chromatin disks is part of its nuclear-envelope ESCRT recruitment function. supported_by: - reference_id: PMID:28242692 supporting_text: CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery - reference_id: PMID:28242692 supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: protein localization to chromatin / chromatin disk periphery where this represents LEMD2-dependent recruitment of CHMP7 and downstream ESCRT factors to the reforming nuclear envelope - term: id: GO:0000815 label: ESCRT III complex evidence_type: NAS original_reference_id: PMID:28242692 qualifier: part_of review: summary: 'Supported CHMP7 ESCRT-III-associated complex annotation: ESCRT III complex.' action: ACCEPT reason: CHMP7 is an ESCRT-III-like factor that recruits and associates with downstream ESCRT-III components, especially at the reforming nuclear envelope. supported_by: - reference_id: PMID:26040712 supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules - reference_id: PMID:28242692 supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope - reference_id: PMID:16856878 supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: CHMP7 is an ESCRT-III-like / ESCRT-II-III hybrid protein with two literature-supported contexts - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:28242692 qualifier: enables review: summary: 'Generic protein binding is over-annotated for CHMP7: protein binding.' action: MARK_AS_OVER_ANNOTATED reason: The meaningful interactions are CHMP7-LEMD2 and CHMP7-CHMP4B; GO:0005515 is too generic to represent CHMP7 function. supported_by: - reference_id: PMID:16856878 supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b - reference_id: PMID:28242692 supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: The meaningful molecular interactions are CHMP7-LEMD2 and CHMP7-CHMP4B, not undifferentiated protein binding. - term: id: GO:0000815 label: ESCRT III complex evidence_type: IDA original_reference_id: PMID:26040712 qualifier: part_of review: summary: 'Supported CHMP7 ESCRT-III-associated complex annotation: ESCRT III complex.' action: ACCEPT reason: CHMP7 is an ESCRT-III-like factor that recruits and associates with downstream ESCRT-III components, especially at the reforming nuclear envelope. supported_by: - reference_id: PMID:26040712 supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules - reference_id: PMID:28242692 supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope - reference_id: PMID:16856878 supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: CHMP7 is an ESCRT-III-like / ESCRT-II-III hybrid protein with two literature-supported contexts - term: id: GO:0005635 label: nuclear envelope evidence_type: IDA original_reference_id: PMID:26040712 qualifier: located_in review: summary: 'Supported core CHMP7 nuclear-envelope function: nuclear envelope.' action: ACCEPT reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for nuclear membrane closure/sealing. supported_by: - reference_id: PMID:26040712 supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules - reference_id: PMID:26040712 supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites - reference_id: PMID:28242692 supporting_text: CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery - reference_id: PMID:28242692 supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro - reference_id: PMID:28242692 supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope - reference_id: UniProt:Q8WUX9 supporting_text: required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure - term: id: GO:0010458 label: exit from mitosis evidence_type: IMP original_reference_id: PMID:26040712 qualifier: involved_in review: summary: 'The cell-cycle/spindle wording is too broad for CHMP7: exit from mitosis.' action: MODIFY reason: CHMP7 contributes specifically to nuclear membrane reassembly and NE sealing during mitotic exit rather than to broad mitotic progression or spindle assembly. supported_by: - reference_id: PMID:26040712 supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites - reference_id: PMID:28242692 supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: exit from mitosis, nuclear pore, chromatin location, and spindle-assembly wording should be interpreted through the specific CHMP7 role in nuclear membrane reassembly and nuclear-envelope sealing proposed_replacement_terms: - id: GO:0031468 label: nuclear membrane reassembly - term: id: GO:0031468 label: nuclear membrane reassembly evidence_type: IMP original_reference_id: PMID:26040712 qualifier: involved_in review: summary: 'Supported core CHMP7 nuclear-envelope function: nuclear membrane reassembly.' action: ACCEPT reason: CHMP7 is recruited by LEMD2/LEM2 to the reforming nuclear envelope and recruits downstream ESCRT-III factors for nuclear membrane closure/sealing. supported_by: - reference_id: PMID:26040712 supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules - reference_id: PMID:26040712 supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites - reference_id: PMID:28242692 supporting_text: CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery - reference_id: PMID:28242692 supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro - reference_id: PMID:28242692 supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope - reference_id: UniProt:Q8WUX9 supporting_text: required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure - term: id: GO:0036258 label: multivesicular body assembly evidence_type: NAS original_reference_id: PMID:20588296 qualifier: involved_in review: summary: 'Supported but secondary CHMP7 endosomal/MVB ESCRT context: multivesicular body assembly.' action: KEEP_AS_NON_CORE reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. supported_by: - reference_id: PMID:16856878 supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway - reference_id: PMID:16856878 supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. - term: id: GO:0039702 label: viral budding via host ESCRT complex evidence_type: NAS original_reference_id: PMID:20588296 qualifier: involved_in review: summary: 'Over-annotated CHMP7 viral-budding term: viral budding via host ESCRT complex.' action: MARK_AS_OVER_ANNOTATED reason: The CHMP7 viral evidence is a dominant-negative overexpression effect on virus-like particle release, not an endogenous core CHMP7 viral-budding function. supported_by: - reference_id: PMID:16856878 supporting_text: dominant-negative effect of overexpressed GFP-CHMP7 - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: The direct evidence is dominant-negative overexpression affecting virus-like particle release, not a demonstrated endogenous core viral-budding function. - term: id: GO:1904903 label: ESCRT III complex disassembly evidence_type: NAS original_reference_id: PMID:20588296 qualifier: involved_in review: summary: 'Supported but secondary CHMP7 endosomal/MVB ESCRT context: ESCRT III complex disassembly.' action: KEEP_AS_NON_CORE reason: CHMP7 has a reported endosomal-sorting phenotype and CHMP4B association, but its strongest CHMP7-specific mechanism is LEMD2-dependent ESCRT recruitment to the reforming nuclear envelope. supported_by: - reference_id: PMID:16856878 supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway - reference_id: PMID:16856878 supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. - term: id: GO:0005829 label: cytosol evidence_type: TAS original_reference_id: Reactome:R-HSA-3159232 qualifier: located_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0005829 label: cytosol evidence_type: TAS original_reference_id: Reactome:R-HSA-917693 qualifier: located_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0005829 label: cytosol evidence_type: TAS original_reference_id: Reactome:R-HSA-917700 qualifier: located_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0005829 label: cytosol evidence_type: TAS original_reference_id: Reactome:R-HSA-9668335 qualifier: located_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0005829 label: cytosol evidence_type: TAS original_reference_id: Reactome:R-HSA-9668389 qualifier: located_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0005829 label: cytosol evidence_type: TAS original_reference_id: Reactome:R-HSA-9668395 qualifier: located_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0005829 label: cytosol evidence_type: TAS original_reference_id: Reactome:R-HSA-9668398 qualifier: located_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0005829 label: cytosol evidence_type: TAS original_reference_id: Reactome:R-HSA-9668405 qualifier: located_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0005829 label: cytosol evidence_type: TAS original_reference_id: Reactome:R-HSA-9668415 qualifier: located_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0005829 label: cytosol evidence_type: TAS original_reference_id: Reactome:R-HSA-9668419 qualifier: located_in review: summary: 'Plausible secondary ESCRT pathway or broad location context for CHMP7: cytosol.' action: KEEP_AS_NON_CORE reason: This annotation is compatible with broader ESCRT biology or observed broad localization, but it is not as specific or central as CHMP7-dependent nuclear-envelope ESCRT recruitment and sealing. supported_by: - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: Keep autophagy/amphisome/lysosomal membrane, plasma membrane repair, cytoplasm/cytosol/nucleoplasm, kinetochore/midbody, and broad ESCRT cell-cycle annotations as non-core pathway contexts - term: id: GO:0000815 label: ESCRT III complex evidence_type: IDA original_reference_id: PMID:16856878 qualifier: part_of review: summary: 'Supported CHMP7 ESCRT-III-associated complex annotation: ESCRT III complex.' action: ACCEPT reason: CHMP7 is an ESCRT-III-like factor that recruits and associates with downstream ESCRT-III components, especially at the reforming nuclear envelope. supported_by: - reference_id: PMID:26040712 supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules - reference_id: PMID:28242692 supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope - reference_id: PMID:16856878 supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: CHMP7 is an ESCRT-III-like / ESCRT-II-III hybrid protein with two literature-supported contexts - term: id: GO:0045324 label: late endosome to vacuole transport evidence_type: IMP original_reference_id: PMID:16856878 qualifier: involved_in review: summary: The endosomal-sorting evidence is real, but late endosome to vacuole transport should be expressed with human MVB/endolysosomal terms. action: MODIFY reason: CHMP7 has an older supported endosomal-sorting phenotype, but vacuole wording is less appropriate for human curation than MVB sorting / late endosome-to-lysosome transport. supported_by: - reference_id: PMID:16856878 supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway - reference_id: PMID:16856878 supporting_text: positive interaction between the C-terminal half of CHMP7 and CHMP4b - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: PMID:16856878 supports an endosomal-sorting phenotype, but the stronger CHMP7-specific mechanism is nuclear-envelope ESCRT recruitment. proposed_replacement_terms: - id: GO:0071985 label: multivesicular body sorting pathway - id: GO:1902774 label: late endosome to lysosome transport references: - id: GO_REF:0000002 title: Gene Ontology annotation through association of InterPro records with GO terms findings: [] - id: GO_REF:0000033 title: Annotation inferences using phylogenetic trees findings: [] - id: GO_REF:0000044 title: Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt findings: [] - id: GO_REF:0000052 title: Gene Ontology annotation based on curation of immunofluorescence data findings: [] - id: GO_REF:0000117 title: Electronic Gene Ontology annotations created by ARBA machine learning models findings: [] - id: PMID:16505166 title: Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a conserved VSL region in Vta1. findings: [] - id: PMID:16554368 title: The ESCRT-III subunit hVps24 is required for degradation but not silencing of the epidermal growth factor receptor. findings: [] - id: PMID:16856878 title: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway. findings: [] - id: PMID:17984323 title: Functional multivesicular bodies are required for autophagic clearance of protein aggregates associated with neurodegenerative disease. findings: [] - id: PMID:19234443 title: Membrane scission by the ESCRT-III complex. findings: [] - id: PMID:20588296 title: 'Membrane budding and scission by the ESCRT machinery: it''s all in the neck.' findings: [] - id: PMID:20616062 title: Human ESCRT-III and VPS4 proteins are required for centrosome and spindle maintenance. findings: [] - id: PMID:24482116 title: ESCRT machinery is required for plasma membrane repair. findings: [] - id: PMID:24878737 title: Structure of cellular ESCRT-III spirals and their relationship to HIV budding. findings: [] - id: PMID:26040712 title: Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing. findings: [] - id: PMID:26040713 title: ESCRT-III controls nuclear envelope reformation. findings: [] - id: PMID:28242692 title: LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells. findings: [] - id: PMID:30194290 title: Interrogating the protein interactomes of RAS isoforms identifies PIP5K1A as a KRAS-specific vulnerability. findings: [] - id: PMID:32814053 title: Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains. findings: [] - id: PMID:36107470 title: Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission. findings: [] - id: Reactome:R-HSA-3159232 title: Recruitment Of HIV Virion Budding Machinery findings: [] - id: Reactome:R-HSA-917693 title: ESCRT Disassembly findings: [] - id: Reactome:R-HSA-917700 title: MVB Vesicle Formation findings: [] - id: Reactome:R-HSA-9668335 title: CHMP7 binds LEMD2 findings: [] - id: Reactome:R-HSA-9668389 title: VPS4 binds ESCRT-III assemblies at nuclear envelope (NE) fenestrations findings: [] - id: Reactome:R-HSA-9668395 title: CHMP7 binds CC2D1B findings: [] - id: Reactome:R-HSA-9668398 title: CHMP7 binds CHMP4B, which recruits other subunits of the ESCRT-III complex findings: [] - id: Reactome:R-HSA-9668405 title: SPAST (spastin) binds the IST1 subunit of ESCRT-III at the sites of microtubule attachment to chromatin findings: [] - id: Reactome:R-HSA-9668415 title: VPS4 mediates disassembly of ESCRTIII subunits to promote sealing of holes in the nuclear envelope findings: [] - id: Reactome:R-HSA-9668419 title: SPAST (spastin) mediates the severing of microtubules at chromosome attachment sites findings: [] - id: UniProt:Q8WUX9 title: UniProtKB entry Q8WUX9 (CHMP7) findings: - statement: CHMP7 recruits ESCRT-III to the nuclear envelope during late anaphase and is recruited there by LEMD2. supporting_text: required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase - id: file:human/CHMP7/CHMP7-notes.md title: CHMP7 review notes findings: - statement: CHMP7 core function is LEMD2-dependent recruitment of ESCRT factors to the reforming nuclear envelope; endosomal sorting is supported but secondary. supporting_text: the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure core_functions: - description: CHMP7 is a LEMD2/LEM2-recruited ESCRT-III-like adaptor that localizes to reforming nuclear-envelope/chromatin-disk sites during late anaphase. It recruits downstream ESCRT-III factors to nuclear-envelope holes, supporting membrane closure/fission-like sealing of the nuclear envelope and coordination with spindle microtubule disassembly. in_complex: id: GO:0000815 label: ESCRT III complex directly_involved_in: - id: GO:0031468 label: nuclear membrane reassembly - id: GO:0071168 label: protein localization to chromatin - id: GO:0090148 label: membrane fission locations: - id: GO:0005635 label: nuclear envelope supported_by: - reference_id: PMID:26040712 supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules - reference_id: PMID:26040712 supporting_text: coordinate nuclear envelope sealing and spindle disassembly at nuclear envelope-microtubule intersection sites - reference_id: PMID:28242692 supporting_text: CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery - reference_id: PMID:28242692 supporting_text: CHMP7 can bind directly to the C-terminal domain of LEM2 in vitro - reference_id: PMID:28242692 supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope - reference_id: UniProt:Q8WUX9 supporting_text: required to recruit the ESCRT-III complex to the nuclear envelope (NE) during late anaphase - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: the core function should emphasize LEMD2/LEM2-dependent recruitment of CHMP7 and downstream ESCRT-III factors to the nuclear envelope for nuclear membrane closure - reference_id: PMID:26040712 supporting_text: ESCRT-III-like protein CHMP7 to sites where the reforming nuclear envelope engulfs spindle microtubules - reference_id: PMID:28242692 supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope - reference_id: PMID:16856878 supporting_text: CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: CHMP7 is an ESCRT-III-like / ESCRT-II-III hybrid protein with two literature-supported contexts - reference_id: PMID:28242692 supporting_text: CHMP7 and LEM2 enrich at the same region of the chromatin disk periphery - reference_id: PMID:28242692 supporting_text: recruits Cmp7p/CHMP7 and downstream ESCRT factors to the nuclear envelope - reference_id: file:human/CHMP7/CHMP7-notes.md supporting_text: protein localization to chromatin / chromatin disk periphery where this represents LEMD2-dependent recruitment of CHMP7 and downstream ESCRT factors to the reforming nuclear envelope proposed_new_terms: [] suggested_questions: - question: Can endogenous CHMP7 rescue assays separate LEMD2 binding, CHMP4B recruitment, and VPS4-dependent ESCRT turnover during nuclear-envelope sealing? - question: Should CHMP7 endosomal-sorting annotations remain non-core unless supported by endogenous depletion/rescue assays distinct from dominant-negative overexpression phenotypes? - question: Is there a more specific GO process term needed for ESCRT-mediated nuclear-envelope hole sealing during anaphase? suggested_experiments: - description: Rescue CHMP7-depleted cells with wild-type CHMP7 and mutants separating LEMD2 binding, CHMP4B binding, and ESCRT-III recruitment while measuring CHMP2A/IST1 recruitment, NE sealing reporters, and post-mitotic nuclear leakage. hypothesis: CHMP7 nuclear-envelope sealing will require LEMD2-dependent recruitment and downstream ESCRT-III assembly more directly than its endosomal-sorting phenotype. - description: Compare acute endogenous CHMP7 depletion/rescue in EGFR or ubiquitinated-cargo MVB sorting assays versus nuclear-envelope sealing assays under matched expression levels. hypothesis: The nuclear-envelope phenotype will be the clearest CHMP7-specific core function, whereas endosomal sorting will be weaker or context-dependent. - description: Test whether CHMP7 perturbation affects virus-like particle release only through overexpression/dominant-negative ESCRT disruption or through a reproducible endogenous requirement. hypothesis: Viral-budding effects will reflect pathway-level ESCRT perturbation rather than a direct core CHMP7 function.