COL5A2 encodes the alpha-2 chain of type-V collagen, a secreted fibrillar extracellular-matrix protein. Its principal assembly contains two alpha-1(V) chains and one alpha-2(V) chain. Type-V collagen participates in heterotypic fibrils with type-I collagen and helps organize their architecture. The alpha-2 chain supports efficient incorporation of type-V collagen into the matrix; disruption of its structure alters fibril assembly and connective-tissue integrity, causing classical Ehlers-Danlos syndrome. Its structural contribution is particularly important in skin and corneal connective tissue. The precursor passes through the secretory pathway before extracellular processing and matrix incorporation.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005201 extracellular matrix structural constituent | IEA GO_REF:0000002 | MODIFY | Summary: Specify the collagen tensile-strength structural class. Reason: The original InterPro IPR000885 collagen C-terminal-domain mapping supports matrix structural activity. Human COL5A2 variants impair type-V assembly, and actual mouse Col5a2 pN-mutant matrix experiments show defective heterotrimer incorporation and abnormal type-I/V fibril organization. The current GO:0030020 comment explicitly permits extracellular-matrix collagens. This refinement does not present proteomic detection as an isolated-chain tensile assay. Propagation Review Root cause: TERM SCOPING PROBLEM Sources checked: InterPro:IPR000885 SUPPORTS TRANSFER Official IPR000885 response maps collagen C-terminal domain to the structural parent. The specific replacement is independently supported by COL5A2 assembly and the current collagen convention. Proposed replacements: extracellular matrix structural constituent conferring tensile strength |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-1474266 | ACCEPT | Summary: Retain the modeled extracellular region location. Reason: The complete event R-HSA-1474266 (Formation of collagen fibrils) has 48 enumerated target routes. A representative input route resolves 3,4-Hyp 5-Gal-Hyl collagen alpha-2(V) chain [extracellular region] to UniProt:P05997. This supports the annotated compartment. Supporting Evidence: Reactome:R-HSA-1474266 |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-1564164 | ACCEPT | Summary: Retain the modeled extracellular region location. Reason: The complete event R-HSA-1564164 (Collagen type V degradation by MMP2,9,10) has 72 enumerated target routes. A representative input route resolves 3,4-Hyp 5-Gal-Hyl collagen alpha-2(V) chain [extracellular region] to UniProt:P05997. This supports the annotated compartment. Supporting Evidence: Reactome:R-HSA-1564164 |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-2002428 | ACCEPT | Summary: Retain the modeled extracellular region location. Reason: The complete event R-HSA-2002428 (Removal of fibrillar collagen N-propeptides) has 48 enumerated target routes. A representative input route resolves 3x4Hyp-3Hyp-GalHyl-COL5A2(27-1499) [extracellular region] to UniProt:P05997. This supports the annotated compartment. Supporting Evidence: Reactome:R-HSA-2002428 |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-2002440 | ACCEPT | Summary: Retain the modeled extracellular region location. Reason: The complete event R-HSA-2002440 (Removal of fibrillar collagen C-propeptides) has 48 enumerated target routes. A representative input route resolves 3x4Hyp-3Hyp-GalHyl-COL5A2(?-1499) [extracellular region] to UniProt:P05997. This supports the annotated compartment. Supporting Evidence: Reactome:R-HSA-2002440 |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-2089971 | ACCEPT | Summary: Retain the modeled extracellular region location. Reason: The complete event R-HSA-2089971 (Secretion of collagens) has 48 enumerated target routes. A representative output route resolves 3x4Hyp-3Hyp-GalHyl-COL5A2(27-1499) [extracellular region] to UniProt:P05997. This supports the annotated compartment. In this secretion event the selected route is the extracellular output, distinct from the ER-lumen input. Supporting Evidence: Reactome:R-HSA-2089971 |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-2213201 | ACCEPT | Summary: Retain the modeled extracellular region location. Reason: The complete event R-HSA-2213201 (Formation of collagen fibres) has 48 enumerated target routes. A representative input route resolves 3,4-Hyp 5-Gal-Hyl collagen alpha-2(V) chain [extracellular region] to UniProt:P05997. This supports the annotated compartment. Supporting Evidence: Reactome:R-HSA-2213201 |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-2327695 | ACCEPT | Summary: Retain the modeled extracellular region location. Reason: The complete event R-HSA-2327695 (Collagen types III, IV, V, VI, VIII, IX, XVI bind integrins alpha1beta1 and alpha2beta1) has 48 enumerated target routes. A representative input route resolves 3,4-Hyp 5-Gal-Hyl collagen alpha-2(V) chain [extracellular region] to UniProt:P05997. This supports the annotated compartment. Supporting Evidence: Reactome:R-HSA-2327695 |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-2327733 | ACCEPT | Summary: Retain the modeled extracellular region location. Reason: The complete event R-HSA-2327733 (FN1 binds Collagen types I-V, VII) has 48 enumerated target routes. A representative input route resolves 3,4-Hyp 5-Gal-Hyl collagen alpha-2(V) chain [extracellular region] to UniProt:P05997. This supports the annotated compartment. Supporting Evidence: Reactome:R-HSA-2327733 |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-2327738 | ACCEPT | Summary: Retain the modeled extracellular region location. Reason: The complete event R-HSA-2327738 (DDR1 binds collagen type I, II, III, IV, V, XI fibrils) has 48 enumerated target routes. A representative input route resolves 3,4-Hyp 5-Gal-Hyl collagen alpha-2(V) chain [extracellular region] to UniProt:P05997. This supports the annotated compartment. Supporting Evidence: Reactome:R-HSA-2327738 |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-2396370 | ACCEPT | Summary: Retain the modeled extracellular region location. Reason: The complete event R-HSA-2396370 (VTN binds collagens II, III and V) has 48 enumerated target routes. A representative input route resolves 3,4-Hyp 5-Gal-Hyl collagen alpha-2(V) chain [extracellular region] to UniProt:P05997. This supports the annotated compartment. Supporting Evidence: Reactome:R-HSA-2396370 |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-2465890 | ACCEPT | Summary: Retain the modeled extracellular region location. Reason: The complete event R-HSA-2465890 (DDR2 binds collagen type I, II, III, V, X fibrils) has 48 enumerated target routes. A representative input route resolves 3,4-Hyp 5-Gal-Hyl collagen alpha-2(V) chain [extracellular region] to UniProt:P05997. This supports the annotated compartment. Supporting Evidence: Reactome:R-HSA-2465890 |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-375151 | ACCEPT | Summary: Retain the modeled extracellular region location. Reason: The complete event R-HSA-375151 (Interaction of NCAM1 with collagens) has 48 enumerated target routes. A representative input route resolves 3,4-Hyp 5-Gal-Hyl collagen alpha-2(V) chain [extracellular region] to UniProt:P05997. This supports the annotated compartment. The event narrative emphasizes other collagen types; the authenticated member route, rather than a newly demonstrated COL5A2-specific NCAM binding assay, supports this location. Supporting Evidence: Reactome:R-HSA-375151 |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-382054 | ACCEPT | Summary: Retain the modeled extracellular region location. Reason: The complete event R-HSA-382054 (PDGF binds to extracellular matrix proteins) has 48 enumerated target routes. A representative input route resolves 3,4-Hyp 5-Gal-Hyl collagen alpha-2(V) chain [extracellular region] to UniProt:P05997. This supports the annotated compartment. Supporting Evidence: Reactome:R-HSA-382054 |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-9638561 | ACCEPT | Summary: Retain the modeled extracellular region location. Reason: The complete event R-HSA-9638561 (MrkD binds Collagen V) has 48 enumerated target routes. A representative input route resolves 3,4-Hyp 5-Gal-Hyl collagen alpha-2(V) chain [extracellular region] to UniProt:P05997. This supports the annotated compartment. Supporting Evidence: Reactome:R-HSA-9638561 |
| GO:0005576 extracellular region | TAS Reactome:R-NUL-2731094 | ACCEPT | Summary: Retain the modeled extracellular region location. Reason: The complete event R-NUL-2731094 (Syndecan-1 binds collagen types I, III, V) has 48 enumerated target routes. A representative input route resolves 3,4-Hyp 5-Gal-Hyl collagen alpha-2(V) chain [extracellular region] to UniProt:P05997. This supports the annotated compartment. This R-NUL event contains a route to the human target but is not itself a new all-human binding experiment. Supporting Evidence: Reactome:R-NUL-2731094 |
| GO:0005581 collagen trimer | IEA GO_REF:0000107 | MODIFY | Summary: Specify the type-V collagen trimer. Reason: The exact mouse Q3U962 and Ensembl protein source resolve to Col5a2; the current same-term record includes IDA evidence from PMID:7704020, whose original experiment was not newly read. Independent human variant evidence and the type-V composition in PMID:21421911 support the more precise alpha1(V)2-alpha2(V) complex. A type-V alpha1 homotrimer observed when alpha2 incorporation fails is not a COL5A2 homotrimer. Propagation Review Root cause: TERM SCOPING PROBLEM Sources checked: UniProtKB:Q3U962 SUPPORTS TRANSFER Official identity and complete exact-term query give 1 current record(s), with evidence IDA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. ensembl:ENSMUSP00000083620 SUPPORTS TRANSFER Official Q3U962 cross-reference resolves ENSMUSP00000083620.5 to mouse Col5a2. The original unversioned source remains unchanged; this identity link is not a new alignment or primary assay. Proposed replacements: collagen type V trimer |
| GO:0005583 fibrillar collagen trimer | IEA GO_REF:0000117 | MODIFY | Summary: Specify the type-V collagen trimer. Reason: The current ARBA00086943 rule includes the target collagen FunFam and Primates branch, supporting a fibrillar collagen class. Independent human variant evidence and the type-V composition in PMID:21421911 support the more precise alpha1(V)2-alpha2(V) complex. A type-V alpha1 homotrimer observed when alpha2 incorporation fails is not a COL5A2 homotrimer. Propagation Review Root cause: TERM SCOPING PROBLEM Sources checked: ARBA:ARBA00086943 SUPPORTS TRANSFER Actual current ARBA00086943 rule was read. Its target collagen-domain/FunFam and primate condition is compatible with the official human record; the rule is not itself an experimental assay. Independent target evidence controls the biological judgment. Proposed replacements: collagen type V trimer |
| GO:0005588 collagen type V trimer | IBA GO_REF:0000033 | ACCEPT | Summary: Retain the inherited type-V trimer assignment. Reason: The target itself has independent experimental type-V records, so its appearance in the PAINT source list is legitimate descendant grounding, not circularity. Human mutation and mouse matrix evidence support its alpha2(V) complex role. Current source records are distinct from the historical PTN002770842 tree, alignment and IBD placement, which were not reconstructed. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN002770842 UNRESOLVED Original ancestral node retained. The historical PAINT tree, alignment and IBD placement were not reconstructed. Current descendant evidence and independent target biology are assessed separately; this is not a donor-count or target-self-evidence objection. UniProtKB:P05997 SUPPORTS TRANSFER Official identity and complete exact-term query give 4 current record(s), with evidence IBA, IMP, NAS. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. The target is a legitimate descendant source; its presence is not circularity. |
| GO:0005588 collagen type V trimer | IMP PMID:9425231 Mutations of the alpha2(V) chain of type V collagen impair m... | ACCEPT | Summary: Retain the experimentally grounded type-V complex assignment. Reason: The complete available original abstract explicitly reports human alpha2(V) mutations, impaired matrix assembly and Ehlers-Danlos syndrome. The original full Methods were not recovered, so no additional reagent or assay detail is attributed to that paper. Independently read mouse Col5a2 matrix and media experiments corroborate the alpha2 chain contribution to type-V heterotrimer incorporation. Supporting Evidence: PMID:9425231 Cultured dermal fibroblasts from both probands produced about equal amounts of the normal and mutant alpha2(V) mRNAs and protein chains. |
| GO:0005588 collagen type V trimer | IMP PMID:9783710 A single base mutation in COL5A2 causes Ehlers-Danlos syndro... | ACCEPT | Summary: Retain the alpha2(V) type-V complex identity. Reason: The original complete abstract identifies the human COL5A2 mutation and abnormal type-V collagen in Ehlers-Danlos syndrome. Its full Methods were not recovered. The explicit type-V composition in the independently read collagen review and actual mouse matrix work support the known complex identity without an all-variant assembly claim. Supporting Evidence: PMID:9783710 Cultured dermal fibroblasts obtained from an affected subject synthesised abnormal type V collagen. |
| GO:0005588 collagen type V trimer | NAS PMID:37158802 The Chemistry and Biology of Collagen Hybridization. | ACCEPT | Summary: Retain the established type-V complex class. Reason: The available full review describes collagen assembly and fibrillar collagen classes; it is supporting synthesis rather than a new isolated human alpha2(V) experiment. The exact type-V identity is independently supported by human mutation evidence and the composition table in PMID:21421911. |
| GO:0005592 collagen type XI trimer | NAS PMID:17876790 The collagen family members as cell adhesion proteins. | UNDECIDED | Summary: The exact type-XI trimer assertion remains unresolved for this source. Reason: GO:0005592 defines alpha1(XI), alpha2(XI) and alpha1(II), whereas COL5A2 encodes alpha2(V). The full PMID:17876790 passage remains unavailable, so its precise NAS assignment cannot be resolved against that definition. V/XI hybrid biology is real: the original PMID:16431952 Introduction describes alpha2(V) with alpha1(XI) in vitreous hybrids, without establishing the strict GO:0005592 composition. Keep this source-specific question UNDECIDED; the available PMID:21421911 Table 1 independently supports a type-V correction for its separate TAS row. |
| GO:0005592 collagen type XI trimer | TAS PMID:21421911 The collagen family. | MODIFY | Summary: Correct this review-supported complex to type V. Reason: Original PMID:21421911 Table 1, accessed in the indexed PMC article, lists alpha2(V) under type V and lists the V/XI hybrid as alpha1(XI), alpha1(V), alpha3(XI). Its accompanying body text likewise identifies alpha1(V) in those hybrids. Current GO:0005592 specifies alpha1(XI), alpha2(XI), alpha1(II); GO:0005588 explicitly includes alpha2(V)-containing type-V heterotrimers. This available source therefore supports MODIFY to type V. The correction does not deny other tissue-specific hybrid assemblies. Proposed replacements: collagen type V trimer Supporting Evidence: PMID:21421911 the Ξ±(XI) chain forms type V/XI hybrid collagen molecules by assembling with the Ξ±1(V) chain |
| GO:0005788 endoplasmic reticulum lumen | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Retain the precursor ER-lumen location. Reason: Current ARBA00028691 includes a Catarrhini/IPR000885 branch concordant with the human target lineage and collagen C-terminal domain. The authenticated Reactome precursor routes independently place COL5A2 in the ER lumen during association and triple-helix formation. This is a biosynthetic compartment, not its final extracellular structural site. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: ARBA:ARBA00028691 SUPPORTS TRANSFER Actual current ARBA00028691 rule was read. Its target collagen-domain/FunFam and primate condition is compatible with the official human record; the rule is not itself an experimental assay. Independent target evidence controls the biological judgment. Supporting Evidence: Reactome:R-HSA-8944223 Reactome:R-HSA-2022073 |
| GO:0005788 endoplasmic reticulum lumen | TAS Reactome:R-HSA-2022073 | KEEP AS NON CORE | Summary: Retain the modeled endoplasmic reticulum lumen location. Reason: The complete event R-HSA-2022073 (Procollagen triple helix formation) has 48 enumerated target routes. A representative input route resolves 3x4Hyp-3Hyp-GalHyl-COL5A2(27-1499) [endoplasmic reticulum lumen] to UniProt:P05997. This supports the annotated compartment. The ER location is a biosynthetic stage rather than the final structural site. Supporting Evidence: Reactome:R-HSA-2022073 |
| GO:0005788 endoplasmic reticulum lumen | TAS Reactome:R-HSA-2089971 | KEEP AS NON CORE | Summary: Retain the modeled endoplasmic reticulum lumen location. Reason: The complete event R-HSA-2089971 (Secretion of collagens) has 48 enumerated target routes. A representative input route resolves 3x4Hyp-3Hyp-GalHyl-COL5A2(27-1499) [endoplasmic reticulum lumen] to UniProt:P05997. This supports the annotated compartment. Here the selected route is the ER-lumen precursor input, distinct from the extracellular secretion output. The ER location is a biosynthetic stage rather than the final structural site. Supporting Evidence: Reactome:R-HSA-2089971 |
| GO:0005788 endoplasmic reticulum lumen | TAS Reactome:R-HSA-8944223 | KEEP AS NON CORE | Summary: Retain the modeled endoplasmic reticulum lumen location. Reason: The complete event R-HSA-8944223 (Association of procollagen type V) has 48 enumerated target routes. A representative input route resolves 3x4Hyp-3Hyp-GalHyl-COL5A2(27-1499) [endoplasmic reticulum lumen] to UniProt:P05997. This supports the annotated compartment. The ER location is a biosynthetic stage rather than the final structural site. Supporting Evidence: Reactome:R-HSA-8944223 |
| GO:0030020 extracellular matrix structural constituent conferring tensile strength | HDA PMID:28344315 Proteomic characterization of human multiple myeloma bone ma... | ACCEPT | Summary: Retain the collagen tensile-strength structural class. Reason: The original bone-marrow ECM S2 row48 and five S3 target rows identify COL5A2/P05997. S2 threshold classifications and S3 individual detections differ: single new-diagnosis and relapsed detections remain positive despite S2 nd. These are not independent isoform or force measurements. Proteomic detection is not a tensile experiment. The current GO:0030020 collagen comment and independent alpha2(V)-dependent matrix assembly support the class without inferring isolated-chain mechanics. |
| GO:0030020 extracellular matrix structural constituent conferring tensile strength | IBA GO_REF:0000033 | ACCEPT | Summary: Retain the inherited matrix structural class. Reason: The current exact P02462 and worm source identities/term records were inspected. Their conserved collagen matrix role is consistent with target-specific type-V structural evidence and the current collagen-term convention. Historical PTN001166351 placement was not reconstructed; neither donor count nor the difference between network and fibrillar collagen architecture is used to invalidate the broader structural class. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN001166351 UNRESOLVED Original ancestral node retained. The historical PAINT tree, alignment and IBD placement were not reconstructed. Current descendant evidence and independent target biology are assessed separately; this is not a donor-count or target-self-evidence objection. UniProtKB:P02462 SUPPORTS TRANSFER Official identity and complete exact-term query give 10 current record(s), with evidence HDA, IBA, IMP, RCA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. WB:WBGene00002280 SUPPORTS TRANSFER Official identity and complete exact-term query give 2 current record(s), with evidence IBA, IMP. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. |
| GO:0030020 extracellular matrix structural constituent conferring tensile strength | RCA PMID:25037231 Extracellular matrix signatures of human primary metastatic ... | ACCEPT | Summary: Retain the collagen tensile-strength structural class. Reason: Both original human colon/liver ECM workbook views identify the P05997-containing COL5A2 protein group at row101, with 95 total unique peptides. Grouped accession assignments do not distinguish separate splice products, and the two workbook views are not independent experiments. Proteomic detection is not a tensile experiment. The current GO:0030020 collagen comment and independent alpha2(V)-dependent matrix assembly support the class without inferring isolated-chain mechanics. |
| GO:0030020 extracellular matrix structural constituent conferring tensile strength | RCA PMID:27068509 Extracellular matrix remodelling in response to venous hyper... | ACCEPT | Summary: Retain the collagen tensile-strength structural class. Reason: Actual human vein extraction/MS Methods and the original Figure 3 caption explicitly identify CO5A2 in the GuHCl comparison. The target workbook was not recovered, and figure pixels or numerical bar values were not inspected. The main-text caption supplies positive target evidence. Proteomic detection is not a tensile experiment. The current GO:0030020 collagen comment and independent alpha2(V)-dependent matrix assembly support the class without inferring isolated-chain mechanics. |
| GO:0030020 extracellular matrix structural constituent conferring tensile strength | RCA PMID:27559042 Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin... | ACCEPT | Summary: Retain the collagen tensile-strength structural class. Reason: Actual original human-heart Table I identifies collagen alpha-2(V) in the GuHCl input. The alpha2(V) row has no positive mark in the enriched glycoprotein or glycopeptide columns; the positive input detection must not be relabeled as those enriched fractions. Proteomic detection is not a tensile experiment. The current GO:0030020 collagen comment and independent alpha2(V)-dependent matrix assembly support the class without inferring isolated-chain mechanics. |
| GO:0030020 extracellular matrix structural constituent conferring tensile strength | RCA PMID:28327460 Comprehensive proteomic characterization of stem cell-derive... | ACCEPT | Summary: Retain the collagen tensile-strength structural class. Reason: Actual original Table I contains the COL5A1/2 collagen-V group in matrices produced by human bone-marrow/adipose stromal cells and neonatal dermal fibroblasts. The group does not uniquely resolve alpha2 peptides or a particular matrix condition. The authors assigned shared human/bovine peptides to human in serum-containing cultures. Proteomic detection is not a tensile experiment. The current GO:0030020 collagen comment and independent alpha2(V)-dependent matrix assembly support the class without inferring isolated-chain mechanics. |
| GO:0030020 extracellular matrix structural constituent conferring tensile strength | RCA PMID:28675934 Characterization of the Extracellular Matrix of Normal and D... | ACCEPT | Summary: Retain the collagen tensile-strength structural class. Reason: The selected source provides human tissue ECM proteomics context, but the exact COL5A2 target supplement was not recovered; attempted workbook and article routes returned HTML challenges. The source-to-target table link remains unverified. Independent original human COL5A2 matrix detections and assembly evidence support the retained biological assertion; no missing table is claimed as read. Proteomic detection is not a tensile experiment. The current GO:0030020 collagen comment and independent alpha2(V)-dependent matrix assembly support the class without inferring isolated-chain mechanics. |
| GO:0030199 collagen fibril organization | IBA GO_REF:0000033 | ACCEPT | Summary: Retain collagen fibril organization through structural assembly. Reason: Current exact descendant records span several collagen chains and include target experimental grounding. The historical PAINT node/MSA/IBD is uninspected. In actual mouse matrix experiments the alpha2(V) defect changes type-V incorporation and type-I/V fibril architecture. COL5A2 contributes chain interfaces and matrix structure to this process rather than merely being a proteolytic substrate. The pN allele retains sparse alpha2 material and does not establish complete absence of secretion in every assay. Propagation Review Root cause: NO FAILURE CORE Sources checked: MGI:MGI:88452 SUPPORTS TRANSFER Official identity and complete exact-term query give 12 current record(s), with evidence IBA, IDA, IEA, IMP, ISO. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. MGI:MGI:88453 SUPPORTS TRANSFER Official identity and complete exact-term query give 11 current record(s), with evidence IBA, IEA, IMP, ISO, NAS. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. MGI:MGI:88458 SUPPORTS TRANSFER Official identity and complete exact-term query give 5 current record(s), with evidence IBA, IEA, IMP, ISO, NAS. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. PANTHER:PTN009074843 UNRESOLVED Original ancestral node retained. The historical PAINT tree, alignment and IBD placement were not reconstructed. Current descendant evidence and independent target biology are assessed separately; this is not a donor-count or target-self-evidence objection. RGD:61817 SUPPORTS TRANSFER Official identity and complete exact-term query give 4 current record(s), with evidence IBA, IDA, IEA, ISO. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. RGD:621351 SUPPORTS TRANSFER Official identity and complete exact-term query give 3 current record(s), with evidence IBA, IDA, ISO. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. UniProtKB:P02458 SUPPORTS TRANSFER Official identity and complete exact-term query give 4 current record(s), with evidence IBA, IDA, IEA, IMP. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. UniProtKB:P02461 SUPPORTS TRANSFER Official identity and complete exact-term query give 6 current record(s), with evidence IBA, IEA, IMP, NAS. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. UniProtKB:P05997 SUPPORTS TRANSFER Official identity and complete exact-term query give 5 current record(s), with evidence IBA, IEA, IMP, ISS, NAS. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. The target is a legitimate descendant source; its presence is not circularity. UniProtKB:P08123 SUPPORTS TRANSFER Official identity and complete exact-term query give 5 current record(s), with evidence IBA, IEA, IMP, NAS. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. ZFIN:ZDB-GENE-030131-9102 SUPPORTS TRANSFER Official identity and complete exact-term query give 2 current record(s), with evidence IBA, IMP. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. |
| GO:0030199 collagen fibril organization | IEA GO_REF:0000120 | ACCEPT | Summary: Retain collagen fibril organization through structural assembly. Reason: Current ARBA00085945 has a matching target FunFam/Primates condition; the mouse Q3U962 and official Ensembl source resolve to Col5a2 with experimental fibril evidence. In actual mouse matrix experiments the alpha2(V) defect changes type-V incorporation and type-I/V fibril architecture. COL5A2 contributes chain interfaces and matrix structure to this process rather than merely being a proteolytic substrate. The pN allele retains sparse alpha2 material and does not establish complete absence of secretion in every assay. Propagation Review Root cause: NO FAILURE CORE Sources checked: ARBA:ARBA00085945 SUPPORTS TRANSFER Actual current ARBA00085945 rule was read. Its target collagen-domain/FunFam and primate condition is compatible with the official human record; the rule is not itself an experimental assay. Independent target evidence controls the biological judgment. UniProtKB:Q3U962 SUPPORTS TRANSFER Official identity and complete exact-term query give 5 current record(s), with evidence IBA, IEA, IMP, ISO, NAS. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. ensembl:ENSMUSP00000083620 SUPPORTS TRANSFER Official Q3U962 cross-reference resolves ENSMUSP00000083620.5 to mouse Col5a2. The original unversioned source remains unchanged; this identity link is not a new alignment or primary assay. |
| GO:0030199 collagen fibril organization | IMP PMID:9425231 Mutations of the alpha2(V) chain of type V collagen impair m... | ACCEPT | Summary: Retain the human matrix-assembly/fibril organization assertion. Reason: The complete original abstract reports alpha2(V) mutations impairing matrix assembly. The full original Methods were not recovered, so its exact fibril measurements are not claimed as newly inspected. Actual independent mouse Col5a2 matrix composition and fibril studies support the structural participation underlying this curated IMP assertion. |
| GO:0030199 collagen fibril organization | ISS GO_REF:0000024 | ACCEPT | Summary: Retain collagen fibril organization through structural assembly. Reason: The exact mouse Col5a2 source has IMP evidence from PMID:15199158, whose selected original Methods/Results were read. In actual mouse matrix experiments the alpha2(V) defect changes type-V incorporation and type-I/V fibril architecture. COL5A2 contributes chain interfaces and matrix structure to this process rather than merely being a proteolytic substrate. The pN allele retains sparse alpha2 material and does not establish complete absence of secretion in every assay. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:Q3U962 SUPPORTS TRANSFER Official identity and complete exact-term query give 5 current record(s), with evidence IBA, IEA, IMP, ISO, NAS. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. |
| GO:0030199 collagen fibril organization | NAS PMID:37158802 The Chemistry and Biology of Collagen Hybridization. | ACCEPT | Summary: Retain the fibril-organization synthesis. Reason: The cited full review describes collagen assembly and fibril architecture, but is not a target-specific perturbation experiment. Actual alpha2(V)-dependent matrix incorporation and heterotypic fibril findings independently establish that COL5A2 supplies structural work in this process. No protease or intrinsic chaperone activity is inferred. |
| GO:0031012 extracellular matrix | HDA PMID:25037231 Extracellular matrix signatures of human primary metastatic ... | ACCEPT | Summary: Retain extracellular-matrix localization. Reason: Both original human colon/liver ECM workbook views identify the P05997-containing COL5A2 protein group at row101, with 95 total unique peptides. Grouped accession assignments do not distinguish separate splice products, and the two workbook views are not independent experiments. Supporting Evidence: |
| GO:0031012 extracellular matrix | HDA PMID:27068509 Extracellular matrix remodelling in response to venous hyper... | ACCEPT | Summary: Retain extracellular-matrix localization. Reason: Actual human vein extraction/MS Methods and the original Figure 3 caption explicitly identify CO5A2 in the GuHCl comparison. The target workbook was not recovered, and figure pixels or numerical bar values were not inspected. The main-text caption supplies positive target evidence. Supporting Evidence: |
| GO:0031012 extracellular matrix | HDA PMID:27559042 Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin... | ACCEPT | Summary: Retain extracellular-matrix localization. Reason: Actual original human-heart Table I identifies collagen alpha-2(V) in the GuHCl input. The alpha2(V) row has no positive mark in the enriched glycoprotein or glycopeptide columns; the positive input detection must not be relabeled as those enriched fractions. Supporting Evidence: |
| GO:0031012 extracellular matrix | HDA PMID:28327460 Comprehensive proteomic characterization of stem cell-derive... | ACCEPT | Summary: Retain extracellular-matrix localization. Reason: Actual original Table I contains the COL5A1/2 collagen-V group in matrices produced by human bone-marrow/adipose stromal cells and neonatal dermal fibroblasts. The group does not uniquely resolve alpha2 peptides or a particular matrix condition. The authors assigned shared human/bovine peptides to human in serum-containing cultures. Supporting Evidence: |
| GO:0031012 extracellular matrix | HDA PMID:28344315 Proteomic characterization of human multiple myeloma bone ma... | ACCEPT | Summary: Retain extracellular-matrix localization. Reason: The original bone-marrow ECM S2 row48 and five S3 target rows identify COL5A2/P05997. S2 threshold classifications and S3 individual detections differ: single new-diagnosis and relapsed detections remain positive despite S2 nd. These are not independent isoform or force measurements. Supporting Evidence: |
| GO:0031012 extracellular matrix | HDA PMID:28675934 Characterization of the Extracellular Matrix of Normal and D... | ACCEPT | Summary: Retain extracellular-matrix localization. Reason: The selected source provides human tissue ECM proteomics context, but the exact COL5A2 target supplement was not recovered; attempted workbook and article routes returned HTML challenges. The source-to-target table link remains unverified. Independent original human COL5A2 matrix detections and assembly evidence support the retained biological assertion; no missing table is claimed as read. |
| GO:0031012 extracellular matrix | IBA GO_REF:0000033 | ACCEPT | Summary: Retain the conserved matrix location. Reason: The complete current source/term joins include experimental extracellular-matrix records on the human target and other collagen products. Target self-evidence is expected descendant grounding, not circularity. The PAINT historical node, alignment and IBD were not inspected. Direct human COL5A2 matrix detections independently support the location while all original source qualifiers remain unchanged. Propagation Review Root cause: NO FAILURE CORE Sources checked: FB:FBgn0000299 SUPPORTS TRANSFER Official identity and complete exact-term query give 1 current record(s), with evidence IBA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. FB:FBgn0016075 SUPPORTS TRANSFER Official identity and complete exact-term query give 4 current record(s), with evidence HDA, IBA, IEA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. MGI:MGI:104687 SUPPORTS TRANSFER Official identity and complete exact-term query give 3 current record(s), with evidence HDA, IBA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. MGI:MGI:104688 SUPPORTS TRANSFER Official identity and complete exact-term query give 4 current record(s), with evidence HDA, IBA, IEA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. MGI:MGI:1858212 SUPPORTS TRANSFER Official identity and complete exact-term query give 5 current record(s), with evidence HDA, IBA, IEA, ISO. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. MGI:MGI:2152695 SUPPORTS TRANSFER Official identity and complete exact-term query give 5 current record(s), with evidence HDA, IBA, IEA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. MGI:MGI:2672118 SUPPORTS TRANSFER Official identity and complete exact-term query give 3 current record(s), with evidence HDA, IBA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. MGI:MGI:2685312 SUPPORTS TRANSFER Official identity and complete exact-term query give 4 current record(s), with evidence HDA, IBA, IEA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. MGI:MGI:88445 SUPPORTS TRANSFER Official identity and complete exact-term query give 7 current record(s), with evidence HDA, IBA, IDA, ISO, NAS. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. MGI:MGI:88446 SUPPORTS TRANSFER Official identity and complete exact-term query give 11 current record(s), with evidence HDA, IBA, IDA, ISO, NAS. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. MGI:MGI:88449 SUPPORTS TRANSFER Official identity and complete exact-term query give 2 current record(s), with evidence HDA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. MGI:MGI:88451 SUPPORTS TRANSFER Official identity and complete exact-term query give 3 current record(s), with evidence HDA, IBA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. MGI:MGI:88452 SUPPORTS TRANSFER Official identity and complete exact-term query give 12 current record(s), with evidence IBA, IDA, IEA, ISO, NAS. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. MGI:MGI:88453 SUPPORTS TRANSFER Official identity and complete exact-term query give 11 current record(s), with evidence HDA, IBA, IDA, IEA, ISO. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. MGI:MGI:88454 SUPPORTS TRANSFER Official identity and complete exact-term query give 6 current record(s), with evidence HDA, IBA, IEA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. MGI:MGI:88455 SUPPORTS TRANSFER Official identity and complete exact-term query give 5 current record(s), with evidence HDA, IBA, IEA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. MGI:MGI:88456 SUPPORTS TRANSFER Official identity and complete exact-term query give 8 current record(s), with evidence HDA, IBA, IEA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. MGI:MGI:88457 SUPPORTS TRANSFER Official identity and complete exact-term query give 11 current record(s), with evidence HDA, IBA, IDA, IEA, ISO, NAS. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. MGI:MGI:88462 SUPPORTS TRANSFER Official identity and complete exact-term query give 2 current record(s), with evidence HDA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. MGI:MGI:88463 SUPPORTS TRANSFER Official identity and complete exact-term query give 2 current record(s), with evidence HDA, IBA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. MGI:MGI:88467 SUPPORTS TRANSFER Official identity and complete exact-term query give 17 current record(s), with evidence HDA, IBA, IDA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. MGI:MGI:88468 SUPPORTS TRANSFER Official identity and complete exact-term query give 5 current record(s), with evidence HDA, IBA, IDA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. PANTHER:PTN008390299 UNRESOLVED Original ancestral node retained. The historical PAINT tree, alignment and IBD placement were not reconstructed. Current descendant evidence and independent target biology are assessed separately; this is not a donor-count or target-self-evidence objection. RGD:2372 SUPPORTS TRANSFER Official identity and complete exact-term query give 3 current record(s), with evidence IBA, IDA, ISO. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. RGD:2374 SUPPORTS TRANSFER Official identity and complete exact-term query give 1 current record(s), with evidence IBA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. RGD:61817 SUPPORTS TRANSFER Official identity and complete exact-term query give 3 current record(s), with evidence IBA, IEA, ISO. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. RGD:621351 SUPPORTS TRANSFER Official identity and complete exact-term query give 2 current record(s), with evidence IBA, ISO. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. RGD:70922 SUPPORTS TRANSFER Official identity and complete exact-term query give 2 current record(s), with evidence IBA, IDA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. RGD:71029 SUPPORTS TRANSFER Official identity and complete exact-term query give 4 current record(s), with evidence IBA, IDA, ISO. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. UniProtKB:P02458 SUPPORTS TRANSFER Official identity and complete exact-term query give 9 current record(s), with evidence HDA, IBA, IDA, IEA, NAS. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. UniProtKB:P02461 SUPPORTS TRANSFER Official identity and complete exact-term query give 11 current record(s), with evidence HDA, IBA, IEA, IMP, TAS. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. UniProtKB:P02462 SUPPORTS TRANSFER Official identity and complete exact-term query give 11 current record(s), with evidence HDA, IBA, ISS. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. UniProtKB:P02467 SUPPORTS TRANSFER Official identity and complete exact-term query give 1 current record(s), with evidence IBA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. UniProtKB:P05997 SUPPORTS TRANSFER Official identity and complete exact-term query give 10 current record(s), with evidence HDA, IBA, NAS. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. The target is a legitimate descendant source; its presence is not circularity. UniProtKB:P08123 SUPPORTS TRANSFER Official identity and complete exact-term query give 10 current record(s), with evidence HDA, IBA, IEA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. UniProtKB:P08572 SUPPORTS TRANSFER Official identity and complete exact-term query give 13 current record(s), with evidence HDA, IBA, ISS, TAS. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. UniProtKB:P12107 SUPPORTS TRANSFER Official identity and complete exact-term query give 5 current record(s), with evidence HDA, IBA, IEA, NAS. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. UniProtKB:P20849 SUPPORTS TRANSFER Official identity and complete exact-term query give 2 current record(s), with evidence HDA, IBA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. UniProtKB:P20908 SUPPORTS TRANSFER Official identity and complete exact-term query give 10 current record(s), with evidence HDA, IBA, IEA, IMP. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. UniProtKB:P29400 SUPPORTS TRANSFER Official identity and complete exact-term query give 3 current record(s), with evidence HDA, IBA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. UniProtKB:P53420 SUPPORTS TRANSFER Official identity and complete exact-term query give 2 current record(s), with evidence HDA, IBA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. UniProtKB:Q01955 SUPPORTS TRANSFER Official identity and complete exact-term query give 5 current record(s), with evidence HDA, IBA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. UniProtKB:Q03692 SUPPORTS TRANSFER Official identity and complete exact-term query give 5 current record(s), with evidence EXP, HDA, IBA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. UniProtKB:Q14031 SUPPORTS TRANSFER Official identity and complete exact-term query give 4 current record(s), with evidence HDA, IBA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. UniProtKB:Q14050 SUPPORTS TRANSFER Official identity and complete exact-term query give 1 current record(s), with evidence HDA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. UniProtKB:Q14055 SUPPORTS TRANSFER Official identity and complete exact-term query give 2 current record(s), with evidence HDA, IBA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. UniProtKB:Q7SIB2 SUPPORTS TRANSFER Official identity and complete exact-term query give 1 current record(s), with evidence IBA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. WB:WBGene00000527 SUPPORTS TRANSFER Official identity and complete exact-term query give 1 current record(s), with evidence IBA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. WB:WBGene00001263 SUPPORTS TRANSFER Official identity and complete exact-term query give 1 current record(s), with evidence IBA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. WB:WBGene00002280 SUPPORTS TRANSFER Official identity and complete exact-term query give 1 current record(s), with evidence IBA. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. |
| GO:0031012 extracellular matrix | NAS PMID:17666000 Downregulation of extracellular matrix-related gene clusters... | ACCEPT | Summary: Retain the independently established matrix location. Reason: The original complete abstract reports downregulation of COL5A2 among extracellular-matrix genes during human stromal-cell differentiation. That expression observation is not itself a protein-localization assay, and the original full Methods were not recovered. Independent direct human ECM detections establish the target location, permitting retention of the curated NAS assertion with this source-specific limit. |
| GO:0031012 extracellular matrix | NAS PMID:17876790 The collagen family members as cell adhesion proteins. | ACCEPT | Summary: Retain the established collagen matrix location. Reason: The complete available review abstract provides collagen cell-adhesion context but does not expose its precise COL5A2 passage; full text was not recovered. Unlike the unresolved type-XI composition assertion from the same source, the broad matrix location is directly corroborated by human COL5A2 proteomics and matrix-assembly evidence. No wrong-gene claim is inferred from the abstract. |
| GO:0043588 skin development | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: Retain skin development as a structural tissue context. Reason: Current exact target and mouse source records support the tissue context; the historical PTN000615820 node was not reconstructed. The alpha2(V)-containing matrix supplies structure during skin development, consistent with abnormal dermal matrix and fibrils. Retain this contextual process without turning disease necessity into a separate signaling or enzymatic activity; the specific fibril-organization role remains the core. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: MGI:MGI:88458 SUPPORTS TRANSFER Official identity and complete exact-term query give 4 current record(s), with evidence IBA, IEA, IMP, ISO. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. PANTHER:PTN000615820 UNRESOLVED Original ancestral node retained. The historical PAINT tree, alignment and IBD placement were not reconstructed. Current descendant evidence and independent target biology are assessed separately; this is not a donor-count or target-self-evidence objection. UniProtKB:P05997 SUPPORTS TRANSFER Official identity and complete exact-term query give 4 current record(s), with evidence IBA, IEA, IMP, ISS. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. The target is a legitimate descendant source; its presence is not circularity. |
| GO:0043588 skin development | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Retain skin development as a structural tissue context. Reason: The mouse Q3U962/Ensembl identity and its direct PMID:15199158 IMP record are verified. The alpha2(V)-containing matrix supplies structure during skin development, consistent with abnormal dermal matrix and fibrils. Retain this contextual process without turning disease necessity into a separate signaling or enzymatic activity; the specific fibril-organization role remains the core. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: UniProtKB:Q3U962 SUPPORTS TRANSFER Official identity and complete exact-term query give 4 current record(s), with evidence IBA, IEA, IMP, ISO. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. ensembl:ENSMUSP00000083620 SUPPORTS TRANSFER Official Q3U962 cross-reference resolves ENSMUSP00000083620.5 to mouse Col5a2. The original unversioned source remains unchanged; this identity link is not a new alignment or primary assay. |
| GO:0043588 skin development | IMP PMID:9425231 Mutations of the alpha2(V) chain of type V collagen impair m... | KEEP AS NON CORE | Summary: Retain skin development as a structural tissue context. Reason: The complete human mutation abstract describes connective-tissue disease and impaired matrix assembly; full original Methods were not recovered. The alpha2(V)-containing matrix supplies structure during skin development, consistent with abnormal dermal matrix and fibrils. Retain this contextual process without turning disease necessity into a separate signaling or enzymatic activity; the specific fibril-organization role remains the core. |
| GO:0043588 skin development | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Retain skin development as a structural tissue context. Reason: The mouse source is Col5a2, with actual neonatal skin and fibroblast matrix experiments in PMID:15199158. The alpha2(V)-containing matrix supplies structure during skin development, consistent with abnormal dermal matrix and fibrils. Retain this contextual process without turning disease necessity into a separate signaling or enzymatic activity; the specific fibril-organization role remains the core. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: UniProtKB:Q3U962 SUPPORTS TRANSFER Official identity and complete exact-term query give 4 current record(s), with evidence IBA, IEA, IMP, ISO. Literal records retain original references, WITH/FROM and qualifiers in the source index. Current records are not a claim that all donor primary papers or historical PAINT placement were read. |
| GO:0046332 SMAD binding | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Retain the ortholog-supported SMAD interaction with assay limits. Reason: GO:0046332 requires binding a SMAD signaling protein; it does not require a demonstrated native signaling pathway. Three current mouse Col5a2 Q3U962 IPI records cite PMID:14559231, and the official Ensembl protein link resolves to that ortholog. The primary abstract reports fetal-mouse-library yeast two-hybrid and GST pull-down binding of collagen V to SMADs. Alpha2(V) attribution rests on those curated donor records because the sequence-defined full assay constructs were not recovered. Retain this positive transferred interaction as non-core, without asserting native human intracellular feedback or rejecting binding solely because mature collagen is extracellular. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: UniProtKB:Q3U962 SUPPORTS TRANSFER Mouse Col5a2 Q3U962 has three exact GO:0046332 IPI records, all citing PMID:14559231 (MGI assignment). This is the documented ortholog evidence behind the Compara transfer. The paper abstract independently reports collagen-V/SMAD binding; alpha2 construct boundaries remain inaccessible. ensembl:ENSMUSP00000083620 SUPPORTS TRANSFER Official Q3U962 cross-reference resolves ENSMUSP00000083620.5 to mouse Col5a2. The original unversioned source remains unchanged; this identity link is not a new alignment or primary assay. Supporting Evidence: PMID:14559231 are capable of binding to the MH2 domain of Smad 3 |
| GO:0048513 animal organ development | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Retain the broad organ-development context. Reason: Current ARBA00029247 includes the target collagen FunFam/Primates branch. Human tissue phenotypes and matrix-assembly biology are compatible with a structural contribution to organ development. The term is broad and does not define a separate molecular activity; no specific developmental signaling pathway is inferred from the rule or phenotype. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: ARBA:ARBA00029247 SUPPORTS TRANSFER Actual current ARBA00029247 rule was read. Its target collagen-domain/FunFam and primate condition is compatible with the official human record; the rule is not itself an experimental assay. Independent target evidence controls the biological judgment. |
| GO:0048592 eye morphogenesis | IMP PMID:16431952 Structural abnormalities of the cornea and lid resulting fro... | KEEP AS NON CORE | Summary: Retain the source-scoped structural eye context. Reason: Actual indexed original human Methods, case table and Results distinguish seven COL5A1-haploinsufficient patients from one patient with a COL5A2 exon-skipping mutation. The latter has corneal/lid abnormalities, supporting contextual structural participation in eye morphology. The mouse corneal experiments in this paper concern Col5a1, not Col5a2. Retain the curated IMP assertion without copying those mouse assays onto alpha2(V), claiming isolated human developmental signaling, or treating the clinical anatomy as a phototransduction experiment. Supporting Evidence: PMID:16431952 one with an exon-skipping mutation in COL5A2 |
| GO:1903225 negative regulation of endodermal cell differentiation | IDA PMID:23154389 Regulation of endodermal differentiation of human embryonic ... | KEEP AS NON CORE | Summary: Retain the measured matrix-dependent differentiation effect. Reason: GO:1903225 covers reduction of endodermal differentiation. Human embryonic-stem-cell microarrays in PMID:23154389 tested commercial whole human collagen V during Wnt3a/Activin-A induction and normalized SOX17 to DNA. The factorial analysis reports a negative collagen-V effect. This is an experimentally applied matrix effect, supporting the existing contextual annotation to its alpha2(V) constituent; it is not an isolated alpha2-chain assay. The receptor and endogenous developmental mechanism remain unresolved, and the separate fibronectin/vitronectin receptor perturbations are not COL5A2 experiments. Supporting Evidence: PMID:23154389 negative effects (e.g. COL V) on DE differentiation |
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Download this section (compressed HTML)Q: Which alpha2(V) interfaces determine incorporation of type-V heterotrimers into type-I/V fibrils, separately from effects on intracellular stability and secretion?
Q: Does the alpha2(V) chain participate in a native SMAD-binding mechanism, and which sequence-defined product and cellular compartment support that interaction?
Q: Which chain and receptor mediate the collagen-V-dependent reduction of endoderm differentiation observed in human stem-cell matrix assays?
Experiment: Compare sequence-defined wild-type and disease-associated human alpha2(V) chains in matched heterotrimer assembly, secretion and matrix-incorporation assays, with chain-resolved mass spectrometry and fibril imaging.
Experiment: Test defined type-V chain compositions in the human stem-cell differentiation assay with receptor perturbations and matched matrix presentation, separating alpha2(V) contribution from whole-collagen effects.
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