CSNK2A1 encodes the catalytic alpha subunit of protein kinase CK2, a broadly expressed protein serine/threonine kinase that often recognizes acidic substrate sequences. It phosphorylates nuclear and cytosolic proteins, both as a catalytic subunit of alpha/beta CK2 assemblies and in alpha-subunit assays. Substrate phosphorylation regulates protein stability, DNA-damage signaling and repair, transcription-associated complexes, chaperone systems and translation. The outcome depends on the substrate and cellular context: CK2 can stabilize one protein while promoting degradation or inhibiting a regulatory interaction in another. The shorter N-terminally truncated product lacks part of the annotated kinase domain and has not been established as catalytically equivalent to the canonical product.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0000785 chromatin | NAS PMID:22325352 PCGF homologs, CBX proteins, and RYBP define functionally di... | KEEP AS NON CORE | Summary: Retain the supported chromatin context as non-core. Reason: The cited PRC1 proteomic study supports a chromatin-associated complex context, rather than a dedicated chromatin structural role for CK2 alpha. Its main text was read but the target-bearing supplemental row was not independently recovered; separate CBX/PRC1 studies establish the positive associated pool. Retain the NAS location as non-core with this access limitation. |
| GO:0004672 protein kinase activity | IEA GO_REF:0000002 | MODIFY | Summary: Refine the supported annotation to protein serine/threonine kinase activity. Reason: The InterPro protein-kinase assertion is sound but broad. Target-level Ser/Thr specificity is established by the human CK2 holoenzyme assay in PMID:11035045 and the official catalytic record; this refinement does not claim that IPR008271 itself establishes a particular substrate or cellular pathway. Propagation Review Root cause: TERM SCOPING PROBLEM Failure modes: GRANULARITY MISMATCH Sources checked: InterPro:IPR008271 SUPPORTS TRANSFER The actual kinase domain supports broad protein kinase activity; the independently established target Ser/Thr class supplies the refinement. Proposed replacements: protein serine/threonine kinase activity |
| GO:0004674 protein serine/threonine kinase activity | IBA GO_REF:0000033 | ACCEPT | Summary: Retain the supported protein serine/threonine kinase activity annotation. Reason: The actual Ser/Thr kinase IBD at PTN000621946 is on the verified 391-aa human target path in the 540-node family. Experimental descendant records, including the target itself, support this catalytic class, which agrees with direct human CK2 chemistry. Target-self evidence is legitimate ancestral grounding, not circularity. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000621946 SUPPORTS TRANSFER The exact term IBD at this proximate ancestor is on the verified target path. Extant source records and sequence identity are retained separately; no donor-count or self-circularity objection applies. |
| GO:0004674 protein serine/threonine kinase activity | IDA PMID:12700239 CK2 phosphorylation of the armadillo repeat region of beta-c... | ACCEPT | Summary: Retain the supported protein serine/threonine kinase activity annotation. Reason: The original PMID:12700239 directly assays recombinant CK2 phosphorylation of beta-catenin, including the Thr393 site. Its cellular inhibition combines alpha and alpha-prime kinase-inactive constructs; the paper does not identify the recombinant kinase insert species or establish an isolated human-alpha perturbation. |
| GO:0004674 protein serine/threonine kinase activity | IDA PMID:15723517 Interactions of the HIV-1 Tat and RAP74 proteins with the RN... | ACCEPT | Summary: Retain the supported protein serine/threonine kinase activity annotation. Reason: The complete PMID:15723517 abstract explicitly reports CK2 phosphorylation of FCP1 and its inhibition by Tat. Full original Methods were not recovered. Retain this established catalytic assertion with curator deference, without inventing the enzyme construct, species or autonomous-alpha assay. |
| GO:0004674 protein serine/threonine kinase activity | IDA PMID:16193064 Caspase-2 primes cancer cells for TRAIL-mediated apoptosis b... | ACCEPT | Summary: Retain the supported protein serine/threonine kinase activity annotation. Reason: Recombinant human CK2 alpha/beta phosphorylates caspase-2 at Ser157; phosphorylation-site mutants and TRAIL-response experiments connect this reaction with caspase activation. Supporting Evidence: PMID:16193064 CK2 phosphorylates procaspase-2 directly at serine-157 |
| GO:0004674 protein serine/threonine kinase activity | IDA PMID:18411307 Phosphorylation of SDT repeats in the MDC1 N terminus trigge... | ACCEPT | Summary: Retain the supported protein serine/threonine kinase activity annotation. Reason: The original MDC1 SDTD-repeat experiments combine CK2-dependent phosphorylation, phosphopeptide recognition and NBS1/MRN recruitment. The performed kinase step is distinct from the downstream DNA-repair machinery. Supporting Evidence: PMID:18411307 CK2 efficiently phosphorylated the WT SDT fragment in vitro |
| GO:0004674 protein serine/threonine kinase activity | IDA PMID:18583988 Phospho-dependent interactions between NBS1 and MDC1 mediate... | ACCEPT | Summary: Retain the supported protein serine/threonine kinase activity annotation. Reason: CK2-dependent phosphorylation of MDC1 SDTD repeats permits NBS1/MRN association. The read main Methods and Results support CK2 chemistry, without claiming that every assay isolates the alpha paralog or that all supplementary experiments were inspected. Supporting Evidence: |
| GO:0004674 protein serine/threonine kinase activity | IDA PMID:18678890 MDC1 regulates intra-S-phase checkpoint by targeting NBS1 to... | ACCEPT | Summary: Retain the supported protein serine/threonine kinase activity annotation. Reason: MDC1 phosphosite mass spectrometry, inhibition and phosphomimetic/binding experiments support the established CK2 catalytic class. The read original main text does not itself supply a newly performed recombinant CK2 assay; that distinction does not invalidate the curator-supported activity. |
| GO:0004674 protein serine/threonine kinase activity | IDA PMID:20545769 Casein kinase 2-dependent phosphorylation of human Rad9 medi... | ACCEPT | Summary: Retain the supported protein serine/threonine kinase activity annotation. Reason: The complete abstract reports CK2 phosphorylation of RAD9 Ser387 and recruitment of TOPBP1; the current GO-CAM explicitly models the CK2 reaction on RAD9. Retain the sound catalytic assertion with curator deference because detailed original assay conditions were not recovered. Supporting Evidence: PMID:20545769 casein kinase 2 (CK2) specifically interacts with human 9-1-1 and phosphorylates serines 341 and 387 |
| GO:0004674 protein serine/threonine kinase activity | IDA PMID:20625391 Functional polymorphism of the CK2alpha intronless gene play... | ACCEPT | Summary: Retain the supported protein serine/threonine kinase activity annotation. Reason: The full original compares CSNK2A1P with authentic human CSNK2A1, including recombinant/cellular kinase controls and PML-related experiments. The title foregrounds the related locus but the annotated human kinase is actually assayed. Supporting Evidence: |
| GO:0004674 protein serine/threonine kinase activity | IDA PMID:20864032 CK2 phospho-dependent binding of R2TP complex to TEL2 is ess... | ACCEPT | Summary: Retain the supported protein serine/threonine kinase activity annotation. Reason: The recovered author manuscript explicitly specifies recombinant CK2 alpha 1 in the kinase assay. TEL2 Ser487/Ser491 peptide and mutant experiments demonstrate substrate phosphorylation; these are not merely an inferred association with the TTT complex. Supporting Evidence: PMID:20864032 on conserved serines 487 and 491 by casein kinase 2 (CK2) |
| GO:0004674 protein serine/threonine kinase activity | IDA PMID:21482717 MDC1 collaborates with TopBP1 in DNA replication checkpoint ... | ACCEPT | Summary: Retain the supported protein serine/threonine kinase activity annotation. Reason: The original RAD9/TOPBP1/MDC1 study uses established CK2-dependent phosphosite recognition to analyze complex assembly. The read Results attribute CK2 phosphorylation to preceding work rather than presenting a new isolated-alpha reaction; the activity remains independently well established and curator-supported. Supporting Evidence: |
| GO:0004674 protein serine/threonine kinase activity | IDA PMID:22017874 Serine 403 phosphorylation of p62/SQSTM1 regulates selective... | ACCEPT | Summary: Retain the supported protein serine/threonine kinase activity annotation. Reason: The complete abstract reports CK2-mediated SQSTM1/p62 Ser403 phosphorylation, enhanced ubiquitin recognition and aggregate clearance. The detailed original Methods remained inaccessible; retain the well-supported kinase class without inventing the construct or assay species. Supporting Evidence: PMID:22017874 Casein kinase 2 (CK2) phosphorylates S403 of p62 directly. |
| GO:0004674 protein serine/threonine kinase activity | IDA PMID:22184066 Phosphorylation of the transcription factor YY1 by CK2Ξ± prev... | ACCEPT | Summary: Retain the supported protein serine/threonine kinase activity annotation. Reason: The complete abstract reports CK2 phosphorylation of YY1 at Ser118, which protects YY1 from caspase-7 cleavage; the authentic GO-CAM preserves this kinase-to-substrate reaction. Full original assay details were not recovered. |
| GO:0004674 protein serine/threonine kinase activity | IDA PMID:22325354 Plk1 and CK2 act in concert to regulate Rad51 during DNA dou... | ACCEPT | Summary: Retain the supported protein serine/threonine kinase activity annotation. Reason: The actual original tests CK2 phosphorylation of RAD51 Thr13 after PLK1-dependent Ser14 priming. Substrate mutations and binding/repair assays distinguish the two kinases and support performed Ser/Thr chemistry. Supporting Evidence: PMID:22325354 S14 phosphorylation licenses subsequent Rad51 phosphorylation at threonine 13 |
| GO:0004674 protein serine/threonine kinase activity | IDA PMID:23123191 Functional interaction of protein kinase CK2 and activating ... | ACCEPT | Summary: Retain the supported protein serine/threonine kinase activity annotation. Reason: The complete abstract reports CK2 interaction with ATF4, nuclear bimolecular fluorescence complementation and ATF4 Ser215 phosphorylation. Full original Methods were not recovered, so construct-level details remain curator-grounded. |
| GO:0004674 protein serine/threonine kinase activity | IDA PMID:24657168 Inositol pyrophosphates mediate the DNA-PK/ATM-p53 cell deat... | ACCEPT | Summary: Retain the supported protein serine/threonine kinase activity annotation. Reason: The recovered normal-fetch full text reports CK2 phosphorylation of the TTT-complex machinery and regulation by IP6. The main plasmid list names CK2 alpha-prime while Results use CK2 alpha, so the construct identity is explicitly unresolved; independent CSNK2A1 assays support the catalytic class. The later correction replaces misassembled figure panels and does not retract the conclusions. |
| GO:0004674 protein serine/threonine kinase activity | IDA PMID:28512243 Plk1 Phosphorylation of Mre11 Antagonizes the DNA Damage Res... | ACCEPT | Summary: Retain the supported protein serine/threonine kinase activity annotation. Reason: The original measures sequential PLK1/CK2 phosphorylation of MRE11, with CK2 Ser688 and PLK1 Ser649 distinguished in Results, and tests DNA binding and repair consequences. A Discussion numbering discrepancy is not silently corrected in the source. Supporting Evidence: |
| GO:0004674 protein serine/threonine kinase activity | IDA PMID:30699359 Post-translational Regulation of FNIP1 Creates a Rheostat fo... | ACCEPT | Summary: Retain the supported protein serine/threonine kinase activity annotation. Reason: The original uses baculovirus-produced CK2 alpha to phosphorylate the FNIP1-D substrate and maps a multisite serine relay with phosphatase and mutation controls. Human-cell association and recombinant chemistry are distinct positive assays. Supporting Evidence: |
| GO:0004674 protein serine/threonine kinase activity | IDA PMID:30765518 Phosphoregulated FMRP phase separation models activity-depen... | ACCEPT | Summary: Retain the supported protein serine/threonine kinase activity annotation. Reason: The original uses CK2 phosphorylation of recombinant FMRP low-complexity fragments before condensate and translation assays, with CK2 subsequently removed. This directly supports kinase chemistry but is not an endogenous human neuronal CK2 knockout experiment. Supporting Evidence: |
| GO:0004674 protein serine/threonine kinase activity | IDA PMID:30898438 MDC1 Interacts with TOPBP1 to Maintain Chromosomal Stability... | ACCEPT | Summary: Retain the supported protein serine/threonine kinase activity annotation. Reason: The original tests constitutive CK2-dependent MDC1 Ser168/Ser196 phosphorylation and TOPBP1 recruitment during mitosis. The phosphorylation is not described as newly induced by DNA damage. Supporting Evidence: |
| GO:0004674 protein serine/threonine kinase activity | IDA PMID:31439799 Phospho-dependent phase separation of FMRP and CAPRIN1 recap... | ACCEPT | Summary: Retain the supported protein serine/threonine kinase activity annotation. Reason: The recovered author paper assays phosphorylated FMRP/CAPRIN1 low-complexity constructs, phase separation, reticulocyte translation and deadenylation. The kinase treatment is positive, with reconstituted substrate and heterologous translation contexts retained. |
| GO:0004674 protein serine/threonine kinase activity | IDA PMID:35597237 A PARylation-phosphorylation cascade promotes TOPBP1 loading... | ACCEPT | Summary: Retain the supported protein serine/threonine kinase activity annotation. Reason: The complete abstract reports CK2 phosphorylation of HTATSF1 Ser748 and TOPBP1-dependent RPA-to-RAD51 exchange; the authentic GO-CAM records this performed reaction. Full primary assay methods were not recovered. |
| GO:0004674 protein serine/threonine kinase activity | IEA GO_REF:0000120 | ACCEPT | Summary: Retain the supported protein serine/threonine kinase activity annotation. Reason: The literal IPR045216/EC2.7.11.1 combined mapping agrees with the official CK2-alpha class and performed Ser/Thr chemistry. Both source identifiers are preserved, without attributing a particular substrate or pathway to the broad mapping. Propagation Review Root cause: NO FAILURE CORE Sources checked: InterPro:IPR045216 SUPPORTS TRANSFER The genuine combined source supports the catalytic class; no new substrate specificity is inferred from this identifier alone. EC:2.7.11.1 SUPPORTS TRANSFER The genuine combined source supports the catalytic class; no new substrate specificity is inferred from this identifier alone. |
| GO:0004674 protein serine/threonine kinase activity | IGI PMID:28031292 Protein kinase CK2 modulates HSJ1 function through phosphory... | ACCEPT | Summary: Retain the supported protein serine/threonine kinase activity annotation. Reason: The original tests CK2 phosphorylation of both HSJ1/DNAJB2 products, including Ser250 priming of Ser247, with purified kinase, cellular association and mutation controls. Monomeric CK2 alpha and holoenzyme assays are distinguished. Supporting Evidence: |
| GO:0004674 protein serine/threonine kinase activity | IMP PMID:11035045 The tumor suppressor PTEN is phosphorylated by the protein k... | ACCEPT | Summary: Retain the supported protein serine/threonine kinase activity annotation. Reason: PMID:11035045 uses commercial human recombinant CK2 holoenzyme with bacterially expressed human PTEN substrates. Mutants and inhibitor experiments support the target catalytic class, with human MCF7 and monkey COS7 cellular contexts distinguished; no isolated-alpha or isoform 2 equivalence is asserted. |
| GO:0004674 protein serine/threonine kinase activity | TAS PMID:2174700 Isolation and characterization of human cDNA clones encoding... | ACCEPT | Summary: Retain the supported protein serine/threonine kinase activity annotation. Reason: The PMID:2174700 abstract establishes human alpha and alpha-prime cDNAs and comparison with bovine subunit peptides, within the stated Ser/Thr kinase family. Full original access remains unavailable; retain this sound TAS class without turning the cDNA comparison into a newly read catalytic assay. Alpha-prime is a separate gene product, not P68400-2. |
| GO:0005515 protein binding | IPI PMID:10094392 Interactions of protein kinase CK2beta subunit within the ho... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:10094392 interaction records identify CSNK2B (P67870) using two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. The beta 180β193 binding-region experiment supports subunit association without defining a new adaptor activity. |
| GO:0005515 protein binding | IPI PMID:11574463 Crystal structure of human protein kinase CK2: insights into... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:11574463 interaction records identify CSNK2B (P67870) using x-ray crystallography. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. The structural record uses alpha 1β337 and beta 1β215; it does not test the complete 391-aa alpha or the 255-aa product. |
| GO:0005515 protein binding | IPI PMID:11972058 Association and regulation of casein kinase 2 activity by ad... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The original PMID:11972058 assertion identifies APC (P25054). No exact pair was recovered in the current MITAB snapshot; that access difference is not evidence that the curator-supported positive interaction is absent. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:12191471 Phosphorylation by protein kinase CK2: a signaling switch fo... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The original PMID:12191471 assertion identifies Nol3 (Q62881). No exact pair was recovered in the current MITAB snapshot; that access difference is not evidence that the curator-supported positive interaction is absent. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. The partner is rat Nol3/ARC; preserve the actual cross-species construct scope. |
| GO:0005515 protein binding | IPI PMID:12769847 Phosphorylation of the WASP-VCA domain increases its affinit... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:12769847 interaction records identify CSNK2B (P67870) using protein kinase assay. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:14667819 Analysis of a high-throughput yeast two-hybrid system and it... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:14667819 interaction records identify CSNK2B (P67870) using two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:16873060 A CK2-dependent mechanism for degradation of the PML tumor s... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:16873060 interaction records identify PML (P29590) using protein kinase assay. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:17309103 Proteomic analysis of SET-binding proteins. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:17309103 interaction records identify SET (Q01105) using protein kinase assay. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:18548200 Transcription factor TAFII250 phosphorylates the acidic doma... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The original PMID:18548200 assertion identifies TAF1 (P21675). No exact pair was recovered in the current MITAB snapshot; that access difference is not evidence that the curator-supported positive interaction is absent. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. TAF1 has several activities and the positive association is retained without choosing one of those activities as the interaction mechanism. |
| GO:0005515 protein binding | IPI PMID:19011756 KIF5C: a new binding partner for protein kinase CK2 with a p... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:19011756 interaction records identify KIF5C (O60282) using anti bait coimmunoprecipitation, cosedimentation through density gradient, fluorescence microscopy, two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. The KIF5C construct is the 1β335 motor fragment. Retain the positive fragment interaction without assuming whole-motor transport activity is mediated by CK2. |
| GO:0005515 protein binding | IPI PMID:19011756 KIF5C: a new binding partner for protein kinase CK2 with a p... | MODIFY | Summary: Refine the supported annotation to protein serine/threonine kinase binding. Reason: The literal PMID:19011756 interaction records identify CSNK2A2 (P19784) using cosedimentation through density gradient, two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The partner is the independently established Ser/Thr kinase CK2 alpha-prime, not the regulatory beta subunit. The more specific term classifies the bound partner; it does not transfer alpha-prime activity to alpha 1 or establish a fixed native heterodimer. Proposed replacements: protein serine/threonine kinase binding |
| GO:0005515 protein binding | IPI PMID:19011756 KIF5C: a new binding partner for protein kinase CK2 with a p... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:19011756 interaction records identify CSNK2B (P67870) using cosedimentation through density gradient, two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:19236849 Carboxy-terminal phosphorylation of SIRT1 by protein kinase ... | MODIFY | Summary: Refine the supported annotation to histone deacetylase binding. Reason: The original PMID:19236849 assertion identifies SIRT1 (Q96EB6). No exact pair was recovered in the current MITAB snapshot; that access difference is not evidence that the curator-supported positive interaction is absent. The named SIRT1/HDAC partner is an established histone deacetylase. Retain the positive interaction with an informative partner-class term, without assigning deacetylase activity to CK2 or upgrading co-complex recovery to purified binary binding. The accessible abstract reports phosphorylation/interaction; absence of a matching current MITAB record is not negative evidence. Proposed replacements: histone deacetylase binding |
| GO:0005515 protein binding | IPI PMID:19294699 Proteomic analysis of GTP cyclohydrolase 1 multiprotein comp... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The original PMID:19294699 assertion identifies GCH1 (P30793). No exact pair was recovered in the current MITAB snapshot; that access difference is not evidence that the curator-supported positive interaction is absent. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:19680552 CK2 is the regulator of SIRT1 substrate-binding affinity, de... | MODIFY | Summary: Refine the supported annotation to histone deacetylase binding. Reason: The original PMID:19680552 assertion identifies SIRT1 (Q96EB6). No exact pair was recovered in the current MITAB snapshot; that access difference is not evidence that the curator-supported positive interaction is absent. The named SIRT1/HDAC partner is an established histone deacetylase. Retain the positive interaction with an informative partner-class term, without assigning deacetylase activity to CK2 or upgrading co-complex recovery to purified binary binding. Proposed replacements: histone deacetylase binding |
| GO:0005515 protein binding | IPI PMID:20439735 SIRT1 regulates Dishevelled proteins and promotes transient ... | MODIFY | Summary: Refine the supported annotation to histone deacetylase binding. Reason: The original PMID:20439735 assertion identifies SIRT1 (Q96EB6). No exact pair was recovered in the current MITAB snapshot; that access difference is not evidence that the curator-supported positive interaction is absent. The named SIRT1/HDAC partner is an established histone deacetylase. Retain the positive interaction with an informative partner-class term, without assigning deacetylase activity to CK2 or upgrading co-complex recovery to purified binary binding. Proposed replacements: histone deacetylase binding |
| GO:0005515 protein binding | IPI PMID:20625391 Functional polymorphism of the CK2alpha intronless gene play... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The original PMID:20625391 assertion identifies PML (P29590). No exact pair was recovered in the current MITAB snapshot; that access difference is not evidence that the curator-supported positive interaction is absent. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:21075308 Recombinant human erythropoietin antagonizes trastuzumab tre... | MODIFY | Summary: Refine the supported annotation to protein tyrosine kinase binding. Reason: The literal PMID:21075308 interaction records identify SRC (P12931) using anti bait coimmunoprecipitation. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. SRC is an independently established protein tyrosine kinase. This refinement identifies the bound partner class without implying tyrosine-kinase activity for CK2 alpha or a unique substrate mechanism. Proposed replacements: protein tyrosine kinase binding |
| GO:0005515 protein binding | IPI PMID:21282530 Interaction proteomics analysis of polycomb proteins defines... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The original PMID:21282530 assertion identifies CBX4 (O00257-3). No exact pair was recovered in the current MITAB snapshot; that access difference is not evidence that the curator-supported positive interaction is absent. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. The full CBX study demonstrates direct CBX4 binding to beta, while alpha is present in nuclear-extract assemblies. Retain the alpha association without a binary-contact claim. |
| GO:0005515 protein binding | IPI PMID:21282530 Interaction proteomics analysis of polycomb proteins defines... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The original PMID:21282530 assertion identifies BMI1 (P35226). No exact pair was recovered in the current MITAB snapshot; that access difference is not evidence that the curator-supported positive interaction is absent. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. The full CBX study detects alpha-associated BMI1 in nuclear-extract pulldowns; this is not proof of purified binary contact. |
| GO:0005515 protein binding | IPI PMID:21282530 Interaction proteomics analysis of polycomb proteins defines... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The original PMID:21282530 assertion identifies CSNK2B (P67870). No exact pair was recovered in the current MITAB snapshot; that access difference is not evidence that the curator-supported positive interaction is absent. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:21282530 Interaction proteomics analysis of polycomb proteins defines... | MODIFY | Summary: Refine the supported annotation to ubiquitin protein ligase binding. Reason: The original PMID:21282530 assertion identifies RNF2 (Q99496). No exact pair was recovered in the current MITAB snapshot; that access difference is not evidence that the curator-supported positive interaction is absent. The named RNF2/RNF111 partner is an established ubiquitin E3 ligase. The term records partner class, not a claim that CK2 ubiquitinates substrates or that every recovered complex represents a direct catalytic-site contact. The full CBX study detects alpha-associated RNF2 in nuclear-extract pulldowns; this is not proof of purified binary contact. Proposed replacements: ubiquitin protein ligase binding |
| GO:0005515 protein binding | IPI PMID:21383183 Casein kinase II-mediated phosphorylation of general repress... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The original PMID:21383183 assertion identifies MAF1 (Q9H063). No exact pair was recovered in the current MITAB snapshot; that access difference is not evidence that the curator-supported positive interaction is absent. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:21516116 Next-generation sequencing to generate interactome datasets. | MODIFY | Summary: Refine the supported annotation to ubiquitin protein ligase binding. Reason: The literal PMID:21516116 interaction records identify RNF111 (Q6ZNA4) using two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The named RNF2/RNF111 partner is an established ubiquitin E3 ligase. The term records partner class, not a claim that CK2 ubiquitinates substrates or that every recovered complex represents a direct catalytic-site contact. Proposed replacements: ubiquitin protein ligase binding |
| GO:0005515 protein binding | IPI PMID:21968188 p53 deacetylation by SIRT1 decreases during protein kinase C... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:21968188 interaction records identify CSNK2B (P67870) using anti bait coimmunoprecipitation. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:21968188 p53 deacetylation by SIRT1 decreases during protein kinase C... | MODIFY | Summary: Refine the supported annotation to histone deacetylase binding. Reason: The literal PMID:21968188 interaction records identify SIRT1 (Q96EB6) using anti bait coimmunoprecipitation. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The named SIRT1/HDAC partner is an established histone deacetylase. Retain the positive interaction with an informative partner-class term, without assigning deacetylase activity to CK2 or upgrading co-complex recovery to purified binary binding. The source distinguishes direct SIRT1 binding through CK2 beta from alpha-containing co-immunoprecipitation; the refinement preserves association scope. Proposed replacements: histone deacetylase binding |
| GO:0005515 protein binding | IPI PMID:21988832 Toward an understanding of the protein interaction network o... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:21988832 interaction records identify CSNK2B (P67870) using two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:22113938 A bead-based approach for large-scale identification of in v... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:22113938 record identifies AIP (O00170) as a target in a protein kinase assay, with interaction type phosphorylation reaction. The original abstract describes cell-lysate proteins immobilized on beads. This is a positive substrate encounter, not an independently measured stable binary-binding affinity; retain the existing broad interaction without adding a specialized binding mechanism from this assay alone. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:22113938 A bead-based approach for large-scale identification of in v... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:22113938 record identifies PRPF40A (O75400-2) as a target in a protein kinase assay, with interaction type phosphorylation reaction. The original abstract describes cell-lysate proteins immobilized on beads. This is a positive substrate encounter, not an independently measured stable binary-binding affinity; retain the existing broad interaction without adding a specialized binding mechanism from this assay alone. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:22113938 A bead-based approach for large-scale identification of in v... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:22113938 record identifies SURF6 (O75683) as a target in a protein kinase assay, with interaction type phosphorylation reaction. The original abstract describes cell-lysate proteins immobilized on beads. This is a positive substrate encounter, not an independently measured stable binary-binding affinity; retain the existing broad interaction without adding a specialized binding mechanism from this assay alone. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:22113938 A bead-based approach for large-scale identification of in v... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:22113938 record identifies EIF3J (O75822) as a target in a protein kinase assay, with interaction type phosphorylation reaction. The original abstract describes cell-lysate proteins immobilized on beads. This is a positive substrate encounter, not an independently measured stable binary-binding affinity; retain the existing broad interaction without adding a specialized binding mechanism from this assay alone. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:22113938 A bead-based approach for large-scale identification of in v... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:22113938 record identifies RPLP1 (P05386) as a target in a protein kinase assay, with interaction type phosphorylation reaction. The original abstract describes cell-lysate proteins immobilized on beads. This is a positive substrate encounter, not an independently measured stable binary-binding affinity; retain the existing broad interaction without adding a specialized binding mechanism from this assay alone. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:22113938 A bead-based approach for large-scale identification of in v... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:22113938 record identifies HSP90AB1 (P08238) as a target in a protein kinase assay, with interaction type phosphorylation reaction. The original abstract describes cell-lysate proteins immobilized on beads. This is a positive substrate encounter, not an independently measured stable binary-binding affinity; retain the existing broad interaction without adding a specialized binding mechanism from this assay alone. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:22113938 A bead-based approach for large-scale identification of in v... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:22113938 record identifies NCL (P19338) as a target in a protein kinase assay, with interaction type phosphorylation reaction. The original abstract describes cell-lysate proteins immobilized on beads. This is a positive substrate encounter, not an independently measured stable binary-binding affinity; retain the existing broad interaction without adding a specialized binding mechanism from this assay alone. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:22113938 A bead-based approach for large-scale identification of in v... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:22113938 record identifies EIF2S2 (P20042) as a target in a protein kinase assay, with interaction type phosphorylation reaction. The original abstract describes cell-lysate proteins immobilized on beads. This is a positive substrate encounter, not an independently measured stable binary-binding affinity; retain the existing broad interaction without adding a specialized binding mechanism from this assay alone. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:22113938 A bead-based approach for large-scale identification of in v... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:22113938 record identifies RPS3 (P23396) as a target in a protein kinase assay, with interaction type phosphorylation reaction. The original abstract describes cell-lysate proteins immobilized on beads. This is a positive substrate encounter, not an independently measured stable binary-binding affinity; retain the existing broad interaction without adding a specialized binding mechanism from this assay alone. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:22113938 A bead-based approach for large-scale identification of in v... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:22113938 record identifies DEK (P35659) as a target in a protein kinase assay, with interaction type phosphorylation reaction. The original abstract describes cell-lysate proteins immobilized on beads. This is a positive substrate encounter, not an independently measured stable binary-binding affinity; retain the existing broad interaction without adding a specialized binding mechanism from this assay alone. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:22113938 A bead-based approach for large-scale identification of in v... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:22113938 record identifies RPS18 (P62269) as a target in a protein kinase assay, with interaction type phosphorylation reaction. The original abstract describes cell-lysate proteins immobilized on beads. This is a positive substrate encounter, not an independently measured stable binary-binding affinity; retain the existing broad interaction without adding a specialized binding mechanism from this assay alone. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:22113938 A bead-based approach for large-scale identification of in v... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:22113938 record identifies SSRP1 (Q08945) as a target in a protein kinase assay, with interaction type phosphorylation reaction. The original abstract describes cell-lysate proteins immobilized on beads. This is a positive substrate encounter, not an independently measured stable binary-binding affinity; retain the existing broad interaction without adding a specialized binding mechanism from this assay alone. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:22113938 A bead-based approach for large-scale identification of in v... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:22113938 record identifies SF3B2 (Q13435) as a target in a protein kinase assay, with interaction type phosphorylation reaction. The original abstract describes cell-lysate proteins immobilized on beads. This is a positive substrate encounter, not an independently measured stable binary-binding affinity; retain the existing broad interaction without adding a specialized binding mechanism from this assay alone. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:22113938 A bead-based approach for large-scale identification of in v... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:22113938 record identifies HDAC1 (Q13547) as a target in a protein kinase assay, with interaction type phosphorylation reaction. The original abstract describes cell-lysate proteins immobilized on beads. This is a positive substrate encounter, not an independently measured stable binary-binding affinity; retain the existing broad interaction without adding a specialized binding mechanism from this assay alone. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:22113938 A bead-based approach for large-scale identification of in v... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:22113938 record identifies HDAC2 (Q92769) as a target in a protein kinase assay, with interaction type phosphorylation reaction. The original abstract describes cell-lysate proteins immobilized on beads. This is a positive substrate encounter, not an independently measured stable binary-binding affinity; retain the existing broad interaction without adding a specialized binding mechanism from this assay alone. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:22113938 A bead-based approach for large-scale identification of in v... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:22113938 record identifies LTV1 (Q96GA3) as a target in a protein kinase assay, with interaction type phosphorylation reaction. The original abstract describes cell-lysate proteins immobilized on beads. This is a positive substrate encounter, not an independently measured stable binary-binding affinity; retain the existing broad interaction without adding a specialized binding mechanism from this assay alone. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:22113938 A bead-based approach for large-scale identification of in v... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:22113938 record identifies DDX24 (Q9GZR7) as a target in a protein kinase assay, with interaction type phosphorylation reaction. The original abstract describes cell-lysate proteins immobilized on beads. This is a positive substrate encounter, not an independently measured stable binary-binding affinity; retain the existing broad interaction without adding a specialized binding mechanism from this assay alone. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:22113938 A bead-based approach for large-scale identification of in v... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:22113938 record identifies PNN (Q9H307) as a target in a protein kinase assay, with interaction type phosphorylation reaction. The original abstract describes cell-lysate proteins immobilized on beads. This is a positive substrate encounter, not an independently measured stable binary-binding affinity; retain the existing broad interaction without adding a specialized binding mechanism from this assay alone. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:22113938 A bead-based approach for large-scale identification of in v... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:22113938 record identifies SUDS3 (Q9H7L9) as a target in a protein kinase assay, with interaction type phosphorylation reaction. The original abstract describes cell-lysate proteins immobilized on beads. This is a positive substrate encounter, not an independently measured stable binary-binding affinity; retain the existing broad interaction without adding a specialized binding mechanism from this assay alone. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:22113938 A bead-based approach for large-scale identification of in v... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:22113938 record identifies THRAP3 (Q9Y2W1) as a target in a protein kinase assay, with interaction type phosphorylation reaction. The original abstract describes cell-lysate proteins immobilized on beads. This is a positive substrate encounter, not an independently measured stable binary-binding affinity; retain the existing broad interaction without adding a specialized binding mechanism from this assay alone. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:22317921 Protein kinase CK2 triggers cytosolic zinc signaling pathway... | MODIFY | Summary: Refine the supported annotation to transmembrane transporter binding. Reason: The literal PMID:22317921 interaction records identify SLC39A7 (Q92504) using anti bait coimmunoprecipitation, anti tag coimmunoprecipitation, fluorescence microscopy, proximity ligation assay. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. ZIP7/SLC39A7 is an experimentally characterized zinc-ion transmembrane transporter. Its interaction with CK2 supports a partner-class refinement; this does not imply that CK2 itself transports zinc or spans the membrane. Proposed replacements: transmembrane transporter binding |
| GO:0005515 protein binding | IPI PMID:22406621 The SUMO E3-ligase PIAS1 regulates the tumor suppressor PML ... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The original PMID:22406621 assertion identifies PML (P29590-12). No exact pair was recovered in the current MITAB snapshot; that access difference is not evidence that the curator-supported positive interaction is absent. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. The source specifies PML isoform 12; the original product-specific assertion remains intact. |
| GO:0005515 protein binding | IPI PMID:23123191 Functional interaction of protein kinase CK2 and activating ... | MODIFY | Summary: Refine the supported annotation to RNA polymerase II-specific DNA-binding transcription factor binding. Reason: The original PMID:23123191 assertion identifies ATF4 (P18848). No exact pair was recovered in the current MITAB snapshot; that access difference is not evidence that the curator-supported positive interaction is absent. The official partner record supports sequence-specific RNA-polymerase-II transcription-factor class. The interaction and partner classification are separate premises: no transcription-factor activity, DNA binding or transcriptional regulation is transferred to CK2. Where the class is phylogenetic, that provenance remains explicit in the evidence index. Proposed replacements: RNA polymerase II-specific DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:23455922 Interlaboratory reproducibility of large-scale human protein... | MODIFY | Summary: Refine the supported annotation to protein serine/threonine kinase binding. Reason: The literal PMID:23455922 interaction records identify CSNK2A2 (P19784) using tandem affinity purification. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The partner is the independently established Ser/Thr kinase CK2 alpha-prime, not the regulatory beta subunit. The more specific term classifies the bound partner; it does not transfer alpha-prime activity to alpha 1 or establish a fixed native heterodimer. Proposed replacements: protein serine/threonine kinase binding |
| GO:0005515 protein binding | IPI PMID:23555304 Dynamic circadian protein-protein interaction networks predi... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:23555304 interaction records identify CSNK2B (P67870) using two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:23752268 The functional interactome landscape of the human histone de... | MODIFY | Summary: Refine the supported annotation to histone deacetylase binding. Reason: The literal PMID:23752268 interaction records identify HDAC1 (Q13547) using anti tag coimmunoprecipitation. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The named SIRT1/HDAC partner is an established histone deacetylase. Retain the positive interaction with an informative partner-class term, without assigning deacetylase activity to CK2 or upgrading co-complex recovery to purified binary binding. Proposed replacements: histone deacetylase binding |
| GO:0005515 protein binding | IPI PMID:24535599 Phosphorylation of Grb14 BPS domain by GSK-3 correlates with... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:24535599 interaction records identify CSNK2B (P67870) using protein kinase assay. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MODIFY | Summary: Refine the supported annotation to RNA polymerase II-specific DNA-binding transcription factor binding. Reason: The literal PMID:25416956 interaction records identify NFYA (P23511) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The official partner record supports sequence-specific RNA-polymerase-II transcription-factor class. The interaction and partner classification are separate premises: no transcription-factor activity, DNA binding or transcriptional regulation is transferred to CK2. Where the class is phylogenetic, that provenance remains explicit in the evidence index. Proposed replacements: RNA polymerase II-specific DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:25416956 interaction records identify MAGEA11 (P43364-2) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. The tested MAGEA11 isoform 2 construct covers 76β394; its fragment interaction is preserved without assigning whole-product coregulator mechanism. |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MODIFY | Summary: Refine the supported annotation to ubiquitin protein ligase binding. Reason: The literal PMID:25416956 interaction records identify RNF111 (Q6ZNA4) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The named RNF2/RNF111 partner is an established ubiquitin E3 ligase. The term records partner class, not a claim that CK2 ubiquitinates substrates or that every recovered complex represents a direct catalytic-site contact. Proposed replacements: ubiquitin protein ligase binding |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:25416956 interaction records identify STAC3 (Q96MF2) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MODIFY | Summary: Refine the supported annotation to RNA polymerase II-specific DNA-binding transcription factor binding. Reason: The literal PMID:25416956 interaction records identify THAP1 (Q9NVV9) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The official partner record supports sequence-specific RNA-polymerase-II transcription-factor class. The interaction and partner classification are separate premises: no transcription-factor activity, DNA binding or transcriptional regulation is transferred to CK2. Where the class is phylogenetic, that provenance remains explicit in the evidence index. Proposed replacements: RNA polymerase II-specific DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:25519132 An AUTS2-Polycomb complex activates gene expression in the C... | MODIFY | Summary: Refine the supported annotation to protein serine/threonine kinase binding. Reason: The original PMID:25519132 assertion identifies CSNK2A2 (P19784). No exact pair was recovered in the current MITAB snapshot; that access difference is not evidence that the curator-supported positive interaction is absent. The partner is the independently established Ser/Thr kinase CK2 alpha-prime, not the regulatory beta subunit. The more specific term classifies the bound partner; it does not transfer alpha-prime activity to alpha 1 or establish a fixed native heterodimer. Proposed replacements: protein serine/threonine kinase binding |
| GO:0005515 protein binding | IPI PMID:25519132 An AUTS2-Polycomb complex activates gene expression in the C... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The original PMID:25519132 assertion identifies CSNK2B (P67870). No exact pair was recovered in the current MITAB snapshot; that access difference is not evidence that the curator-supported positive interaction is absent. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:25852190 Integrative analysis of kinase networks in TRAIL-induced apo... | MODIFY | Summary: Refine the supported annotation to translation initiation factor binding. Reason: The literal PMID:25852190 interaction records identify EIF3J (O75822) using anti tag coimmunoprecipitation. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. EIF3J is a translation-initiation-factor subunit. The polypeptide-binding term fits the verified partner identity; binding to a complete initiation-factor complex or regulation of translation is not inferred from this interaction alone. Proposed replacements: translation initiation factor binding |
| GO:0005515 protein binding | IPI PMID:25852190 Integrative analysis of kinase networks in TRAIL-induced apo... | MODIFY | Summary: Refine the supported annotation to protein serine/threonine kinase binding. Reason: The literal PMID:25852190 interaction records identify CSNK2A2 (P19784) using anti tag coimmunoprecipitation. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The partner is the independently established Ser/Thr kinase CK2 alpha-prime, not the regulatory beta subunit. The more specific term classifies the bound partner; it does not transfer alpha-prime activity to alpha 1 or establish a fixed native heterodimer. Proposed replacements: protein serine/threonine kinase binding |
| GO:0005515 protein binding | IPI PMID:25852190 Integrative analysis of kinase networks in TRAIL-induced apo... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:25852190 interaction records identify CSNK2B (P67870) using anti tag coimmunoprecipitation. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:25852190 Integrative analysis of kinase networks in TRAIL-induced apo... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:25852190 interaction records identify SSRP1 (Q08945) using anti tag coimmunoprecipitation. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:26711270 A Novel Interaction of Ecdysoneless (ECD) Protein with R2TP ... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The original PMID:26711270 assertion identifies CSNK2B (P67870). No exact pair was recovered in the current MITAB snapshot; that access difference is not evidence that the curator-supported positive interaction is absent. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:27705803 A High-Density Map for Navigating the Human Polycomb Complex... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:27705803 interaction records identify SURF6 (O75683) using tandem affinity purification. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:27705803 A High-Density Map for Navigating the Human Polycomb Complex... | MODIFY | Summary: Refine the supported annotation to protein serine/threonine kinase binding. Reason: The literal PMID:27705803 interaction records identify CSNK2A2 (P19784) using tandem affinity purification. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The partner is the independently established Ser/Thr kinase CK2 alpha-prime, not the regulatory beta subunit. The more specific term classifies the bound partner; it does not transfer alpha-prime activity to alpha 1 or establish a fixed native heterodimer. Proposed replacements: protein serine/threonine kinase binding |
| GO:0005515 protein binding | IPI PMID:27705803 A High-Density Map for Navigating the Human Polycomb Complex... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:27705803 interaction records identify CSNK2B (P67870) using tandem affinity purification. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:27705803 A High-Density Map for Navigating the Human Polycomb Complex... | MODIFY | Summary: Refine the supported annotation to ubiquitin protein ligase binding. Reason: The literal PMID:27705803 interaction records identify RNF2 (Q99496) using tandem affinity purification. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The named RNF2/RNF111 partner is an established ubiquitin E3 ligase. The term records partner class, not a claim that CK2 ubiquitinates substrates or that every recovered complex represents a direct catalytic-site contact. Proposed replacements: ubiquitin protein ligase binding |
| GO:0005515 protein binding | IPI PMID:28031292 Protein kinase CK2 modulates HSJ1 function through phosphory... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The original PMID:28031292 assertion identifies DNAJB2 (P25686). No exact pair was recovered in the current MITAB snapshot; that access difference is not evidence that the curator-supported positive interaction is absent. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. Actual DNAJB2 phosphorylation/client-handling experiments support this positive substrate association; a dedicated kinase chaperone-binding core is not inferred. |
| GO:0005515 protein binding | IPI PMID:28031292 Protein kinase CK2 modulates HSJ1 function through phosphory... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The original PMID:28031292 assertion identifies DNAJB2 (P25686-1). No exact pair was recovered in the current MITAB snapshot; that access difference is not evidence that the curator-supported positive interaction is absent. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. The tested DNAJB2 product is explicitly retained, with actual phosphorylation and client-binding context. |
| GO:0005515 protein binding | IPI PMID:28031292 Protein kinase CK2 modulates HSJ1 function through phosphory... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The original PMID:28031292 assertion identifies DNAJB2 (P25686-2). No exact pair was recovered in the current MITAB snapshot; that access difference is not evidence that the curator-supported positive interaction is absent. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. The tested DNAJB2 product is explicitly retained, with actual phosphorylation and client-binding context. |
| GO:0005515 protein binding | IPI PMID:28514442 Architecture of the human interactome defines protein commun... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:28514442 interaction records identify PRPF40A (O75400-2) using anti tag coimmunoprecipitation. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:28514442 Architecture of the human interactome defines protein commun... | MODIFY | Summary: Refine the supported annotation to protein serine/threonine kinase binding. Reason: The literal PMID:28514442 interaction records identify CSNK2A2 (P19784) using anti tag coimmunoprecipitation. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The partner is the independently established Ser/Thr kinase CK2 alpha-prime, not the regulatory beta subunit. The more specific term classifies the bound partner; it does not transfer alpha-prime activity to alpha 1 or establish a fixed native heterodimer. Proposed replacements: protein serine/threonine kinase binding |
| GO:0005515 protein binding | IPI PMID:28514442 Architecture of the human interactome defines protein commun... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:28514442 interaction records identify SSRP1 (Q08945) using anti tag coimmunoprecipitation. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:28514442 Architecture of the human interactome defines protein commun... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:28514442 interaction records identify DVL3 (Q92997) using anti tag coimmunoprecipitation. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:29892012 An interactome perturbation framework prioritizes damaging m... | MODIFY | Summary: Refine the supported annotation to RNA polymerase II-specific DNA-binding transcription factor binding. Reason: The literal PMID:29892012 interaction records identify NFYA (P23511) using two hybrid array. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The official partner record supports sequence-specific RNA-polymerase-II transcription-factor class. The interaction and partner classification are separate premises: no transcription-factor activity, DNA binding or transcriptional regulation is transferred to CK2. Where the class is phylogenetic, that provenance remains explicit in the evidence index. Proposed replacements: RNA polymerase II-specific DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:29892012 An interactome perturbation framework prioritizes damaging m... | MODIFY | Summary: Refine the supported annotation to ubiquitin protein ligase binding. Reason: The literal PMID:29892012 interaction records identify RNF111 (Q6ZNA4) using two hybrid array. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The named RNF2/RNF111 partner is an established ubiquitin E3 ligase. The term records partner class, not a claim that CK2 ubiquitinates substrates or that every recovered complex represents a direct catalytic-site contact. Proposed replacements: ubiquitin protein ligase binding |
| GO:0005515 protein binding | IPI PMID:29892012 An interactome perturbation framework prioritizes damaging m... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:29892012 interaction records identify STAC3 (Q96MF2) using two hybrid array. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:29892012 An interactome perturbation framework prioritizes damaging m... | MODIFY | Summary: Refine the supported annotation to RNA polymerase II-specific DNA-binding transcription factor binding. Reason: The literal PMID:29892012 interaction records identify THAP1 (Q9NVV9) using two hybrid array. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The official partner record supports sequence-specific RNA-polymerase-II transcription-factor class. The interaction and partner classification are separate premises: no transcription-factor activity, DNA binding or transcriptional regulation is transferred to CK2. Where the class is phylogenetic, that provenance remains explicit in the evidence index. Proposed replacements: RNA polymerase II-specific DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:29997244 LuTHy: a double-readout bioluminescence-based two-hybrid tec... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:29997244 interaction records identify CSNK2B (P67870) using bioluminescence resonance energy transfer, luminescence based mammalian interactome mapping. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:30699359 Post-translational Regulation of FNIP1 Creates a Rheostat fo... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The original PMID:30699359 assertion identifies FNIP1 (Q8TF40). No exact pair was recovered in the current MITAB snapshot; that access difference is not evidence that the curator-supported positive interaction is absent. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:31980649 Extensive rewiring of the EGFR network in colorectal cancer ... | MODIFY | Summary: Refine the supported annotation to protein tyrosine kinase binding. Reason: The literal PMID:31980649 interaction records identify SRC (P12931) using anti tag coimmunoprecipitation. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. SRC is an independently established protein tyrosine kinase. This refinement identifies the bound partner class without implying tyrosine-kinase activity for CK2 alpha or a unique substrate mechanism. Proposed replacements: protein tyrosine kinase binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify FCHO1 (A0A0C3SFZ9) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify JADE2 (A0A0C4DFT8) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the supported annotation to RNA polymerase II-specific DNA-binding transcription factor binding. Reason: The literal PMID:32296183 interaction records identify GSC2 (O15499) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The official partner record supports sequence-specific RNA-polymerase-II transcription-factor class. The interaction and partner classification are separate premises: no transcription-factor activity, DNA binding or transcriptional regulation is transferred to CK2. Where the class is phylogenetic, that provenance remains explicit in the evidence index. Proposed replacements: RNA polymerase II-specific DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the supported annotation to RNA polymerase II-specific DNA-binding transcription factor binding. Reason: The literal PMID:32296183 interaction records identify SNAI2 (O43623) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The official partner record supports sequence-specific RNA-polymerase-II transcription-factor class. The interaction and partner classification are separate premises: no transcription-factor activity, DNA binding or transcriptional regulation is transferred to CK2. Where the class is phylogenetic, that provenance remains explicit in the evidence index. Proposed replacements: RNA polymerase II-specific DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the supported annotation to RNA polymerase II-specific DNA-binding transcription factor binding. Reason: The literal PMID:32296183 interaction records identify HOXC4 (P09017) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The official partner record supports sequence-specific RNA-polymerase-II transcription-factor class. The interaction and partner classification are separate premises: no transcription-factor activity, DNA binding or transcriptional regulation is transferred to CK2. Where the class is phylogenetic, that provenance remains explicit in the evidence index. Proposed replacements: RNA polymerase II-specific DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify NFYA (P23511-2) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. The partner is NFYA isoform 2; no independent isoform-specific transcription-factor assay is claimed in this screen, so retain the original broad positive interaction. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the supported annotation to RNA polymerase II-specific DNA-binding transcription factor binding. Reason: The literal PMID:32296183 interaction records identify PAX6 (P26367) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The official partner record supports sequence-specific RNA-polymerase-II transcription-factor class. The interaction and partner classification are separate premises: no transcription-factor activity, DNA binding or transcriptional regulation is transferred to CK2. Where the class is phylogenetic, that provenance remains explicit in the evidence index. Proposed replacements: RNA polymerase II-specific DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the supported annotation to RNA polymerase II-specific DNA-binding transcription factor binding. Reason: The literal PMID:32296183 interaction records identify MEOX1 (P50221) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The official partner record supports sequence-specific RNA-polymerase-II transcription-factor class. The interaction and partner classification are separate premises: no transcription-factor activity, DNA binding or transcriptional regulation is transferred to CK2. Where the class is phylogenetic, that provenance remains explicit in the evidence index. Proposed replacements: RNA polymerase II-specific DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify CSNK2B (P67870) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify PIP4K2B (P78356-2) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. The partner is PIP4K2B isoform 2; the screen alone does not resolve which functional class applies to this exact product. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive POU6F2 interaction as non-core with the tested product limit. Reason: Three positive HuRI records identify the P78424 backbone and explicitly test residues 9β691 of displayed POU6F2 isoform 1. The official product record distinguishes this product, which does not bind DNA, from isoform 2 lacking residues 524β559. The tested fragment retains that insertion. Thus the physical association is supported, but gene-level DNA-binding transcription-factor annotations do not establish that class for this assayed product. Preserve generic binding as non-core; neither a native transcriptional mechanism nor isoform 2 activity is inferred. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the supported annotation to RNA polymerase II-specific DNA-binding transcription factor binding. Reason: The literal PMID:32296183 interaction records identify PAX5 (Q02548) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The official partner record supports sequence-specific RNA-polymerase-II transcription-factor class. The interaction and partner classification are separate premises: no transcription-factor activity, DNA binding or transcriptional regulation is transferred to CK2. Where the class is phylogenetic, that provenance remains explicit in the evidence index. Proposed replacements: RNA polymerase II-specific DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify REL (Q04864-2) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. The partner is REL isoform 2; the exact product interaction is positive, while its product-specific functional class is not newly inferred here. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify TLE5 (Q08117-2) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify ZNF177 (Q13360-2) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. The partner is ZNF177 isoform 2; a zinc-finger name alone is not used to manufacture a DNA-binding-transcription-factor refinement. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify KEAP1 (Q14145) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify RBM39 (Q14498) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify CT45A1 (Q5HYN5) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify PHF19 (Q5T6S3) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify KIF24 (Q5T7B8-2) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. The KIF24 isoform 2 construct covers 385β1234; retain the fragment interaction without an intact motor-activity assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the supported annotation to RNA polymerase II-specific DNA-binding transcription factor binding. Reason: The literal PMID:32296183 interaction records identify LHX8 (Q68G74) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The official partner record supports sequence-specific RNA-polymerase-II transcription-factor class. The interaction and partner classification are separate premises: no transcription-factor activity, DNA binding or transcriptional regulation is transferred to CK2. Where the class is phylogenetic, that provenance remains explicit in the evidence index. Proposed replacements: RNA polymerase II-specific DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify MEOX2 (Q6FHY5) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. The exact MEOX2 accession is an unreviewed record; preserve the positive interaction without treating a domain-only electronic class as an independently verified product function. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify ZSCAN25 (Q6NSZ9-2) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. The tested ZSCAN25 isoform 2 fragment covers 8β472; broad positive binding is retained without an unsupported class-specific mechanism. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify ATN1 (Q86V38) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the supported annotation to RNA polymerase II-specific DNA-binding transcription factor binding. Reason: The literal PMID:32296183 interaction records identify ZNF440 (Q8IYI8) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The official partner record supports sequence-specific RNA-polymerase-II transcription-factor class. The interaction and partner classification are separate premises: no transcription-factor activity, DNA binding or transcriptional regulation is transferred to CK2. For ZNF440 (Q8IYI8), the Pol II DNA-binding transcription-factor class is specifically a reviewed UniProt GO:0000981 IBA:GO_Central assertion, not a direct DNA-binding experiment in this interaction paper. Proposed replacements: RNA polymerase II-specific DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify CCDC116 (Q8IYX3) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify BEND7 (Q8N7W2-2) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. The tested BEND7 isoform 2 fragment covers 10β374; broad positive binding is retained without an unsupported class-specific mechanism. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify FAM133A (Q8N9E0) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify TRIM41 (Q8WV44) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. The TRIM41 construct begins at 86, so the positive screen is retained without assigning E3-ligase binding from a full-protein function when the tested fragment omits its N-terminal RING region. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify DVL3 (Q92997) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify STAC3 (Q96MF2) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. The tested STAC3 construct covers 40β364; retain the association without inferring a calcium-channel or muscle function for CK2. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify TEPSIN (Q96N21) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the supported annotation to RNA polymerase II-specific DNA-binding transcription factor binding. Reason: The literal PMID:32296183 interaction records identify ZNF558 (Q96NG5) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The official partner record supports sequence-specific RNA-polymerase-II transcription-factor class. The interaction and partner classification are separate premises: no transcription-factor activity, DNA binding or transcriptional regulation is transferred to CK2. For ZNF558 (Q96NG5), the Pol II DNA-binding transcription-factor class is specifically a reviewed UniProt GO:0000981 IBA:GO_Central assertion, not a direct DNA-binding experiment in this interaction paper. Proposed replacements: RNA polymerase II-specific DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the supported annotation to RNA polymerase II-specific DNA-binding transcription factor binding. Reason: The literal PMID:32296183 interaction records identify ZNF670 (Q9BS34) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The official partner record supports sequence-specific RNA-polymerase-II transcription-factor class. The interaction and partner classification are separate premises: no transcription-factor activity, DNA binding or transcriptional regulation is transferred to CK2. For ZNF670 (Q9BS34), the Pol II DNA-binding transcription-factor class is specifically a reviewed UniProt GO:0000981 IBA:GO_Central assertion, not a direct DNA-binding experiment in this interaction paper. Proposed replacements: RNA polymerase II-specific DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify MMTAG2 (Q9BU76) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify TSPYL2 (Q9H2G4) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the supported annotation to RNA polymerase II-specific DNA-binding transcription factor binding. Reason: The literal PMID:32296183 interaction records identify LHX9 (Q9NQ69) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The official partner record supports sequence-specific RNA-polymerase-II transcription-factor class. The interaction and partner classification are separate premises: no transcription-factor activity, DNA binding or transcriptional regulation is transferred to CK2. Where the class is phylogenetic, that provenance remains explicit in the evidence index. Proposed replacements: RNA polymerase II-specific DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the supported annotation to RNA polymerase II-specific DNA-binding transcription factor binding. Reason: The literal PMID:32296183 interaction records identify THAP1 (Q9NVV9) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The official partner record supports sequence-specific RNA-polymerase-II transcription-factor class. The interaction and partner classification are separate premises: no transcription-factor activity, DNA binding or transcriptional regulation is transferred to CK2. Where the class is phylogenetic, that provenance remains explicit in the evidence index. Proposed replacements: RNA polymerase II-specific DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify GPATCH2 (Q9NW75-2) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify GPATCH2L (Q9NWQ4-1) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32296183 interaction records identify LHX3 (Q9UBR4-2) using two hybrid array, two hybrid prey pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. The partner is LHX3 isoform 2; retain the exact product interaction without a new product-specific functional-equivalence claim. |
| GO:0005515 protein binding | IPI PMID:32707033 Kinase Interaction Network Expands Functional and Disease Ro... | MODIFY | Summary: Refine the supported annotation to translation initiation factor binding. Reason: The literal PMID:32707033 interaction records identify EIF3J (O75822) using pull down. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. EIF3J is a translation-initiation-factor subunit. The polypeptide-binding term fits the verified partner identity; binding to a complete initiation-factor complex or regulation of translation is not inferred from this interaction alone. Proposed replacements: translation initiation factor binding |
| GO:0005515 protein binding | IPI PMID:32707033 Kinase Interaction Network Expands Functional and Disease Ro... | MODIFY | Summary: Refine the supported annotation to protein serine/threonine kinase binding. Reason: The literal PMID:32707033 interaction records identify CSNK2A2 (P19784) using pull down. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The partner is the independently established Ser/Thr kinase CK2 alpha-prime, not the regulatory beta subunit. The more specific term classifies the bound partner; it does not transfer alpha-prime activity to alpha 1 or establish a fixed native heterodimer. Proposed replacements: protein serine/threonine kinase binding |
| GO:0005515 protein binding | IPI PMID:32707033 Kinase Interaction Network Expands Functional and Disease Ro... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32707033 interaction records identify DEK (P35659) using pull down. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:32707033 Kinase Interaction Network Expands Functional and Disease Ro... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32707033 interaction records identify CSNK2B (P67870) using pull down. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:32707033 Kinase Interaction Network Expands Functional and Disease Ro... | MODIFY | Summary: Refine the supported annotation to histone deacetylase binding. Reason: The literal PMID:32707033 interaction records identify HDAC1 (Q13547) using pull down. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The named SIRT1/HDAC partner is an established histone deacetylase. Retain the positive interaction with an informative partner-class term, without assigning deacetylase activity to CK2 or upgrading co-complex recovery to purified binary binding. Proposed replacements: histone deacetylase binding |
| GO:0005515 protein binding | IPI PMID:32707033 Kinase Interaction Network Expands Functional and Disease Ro... | MODIFY | Summary: Refine the supported annotation to ubiquitin protein ligase binding. Reason: The literal PMID:32707033 interaction records identify RNF111 (Q6ZNA4) using pull down. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The named RNF2/RNF111 partner is an established ubiquitin E3 ligase. The term records partner class, not a claim that CK2 ubiquitinates substrates or that every recovered complex represents a direct catalytic-site contact. Proposed replacements: ubiquitin protein ligase binding |
| GO:0005515 protein binding | IPI PMID:32707033 Kinase Interaction Network Expands Functional and Disease Ro... | MODIFY | Summary: Refine the supported annotation to ubiquitin protein ligase binding. Reason: The literal PMID:32707033 interaction records identify RNF2 (Q99496) using pull down. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The named RNF2/RNF111 partner is an established ubiquitin E3 ligase. The term records partner class, not a claim that CK2 ubiquitinates substrates or that every recovered complex represents a direct catalytic-site contact. Proposed replacements: ubiquitin protein ligase binding |
| GO:0005515 protein binding | IPI PMID:32707033 Kinase Interaction Network Expands Functional and Disease Ro... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32707033 interaction records identify MMTAG2 (Q9BU76) using pull down. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:32707033 Kinase Interaction Network Expands Functional and Disease Ro... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32707033 interaction records identify DDX24 (Q9GZR7) using pull down. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:32707033 Kinase Interaction Network Expands Functional and Disease Ro... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32707033 interaction records identify TSPYL2 (Q9H2G4) using pull down. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:32707033 Kinase Interaction Network Expands Functional and Disease Ro... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32707033 interaction records identify PNN (Q9H307) using pull down. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:32707033 Kinase Interaction Network Expands Functional and Disease Ro... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32707033 interaction records identify THRAP3 (Q9Y2W1) using pull down. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32814053 interaction records identify APP (P05067) using two hybrid array, two hybrid pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. The APP record specifies the 670β671 mutation; preserve the tested mutant association rather than silently calling it wild-type APP. |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32814053 interaction records identify CSNK2B (P67870) using luminescence based mammalian interactome mapping. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32814053 interaction records identify ADARB1 (P78563) using two hybrid array, two hybrid pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32814053 interaction records identify ATN1 (Q86V38) using two hybrid array, two hybrid pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:32814053 interaction records identify KLK6 (Q92876) using two hybrid array, two hybrid pooling approach, validated two hybrid. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | MODIFY | Summary: Refine the supported annotation to translation initiation factor binding. Reason: The literal PMID:33961781 interaction records identify EIF3J (O75822) using anti tag coimmunoprecipitation. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. EIF3J is a translation-initiation-factor subunit. The polypeptide-binding term fits the verified partner identity; binding to a complete initiation-factor complex or regulation of translation is not inferred from this interaction alone. Proposed replacements: translation initiation factor binding |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | MODIFY | Summary: Refine the supported annotation to protein serine/threonine kinase binding. Reason: The literal PMID:33961781 interaction records identify CSNK2A2 (P19784) using anti tag coimmunoprecipitation. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The partner is the independently established Ser/Thr kinase CK2 alpha-prime, not the regulatory beta subunit. The more specific term classifies the bound partner; it does not transfer alpha-prime activity to alpha 1 or establish a fixed native heterodimer. Proposed replacements: protein serine/threonine kinase binding |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:33961781 interaction records identify CSNK2B (P67870) using anti tag coimmunoprecipitation. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | MODIFY | Summary: Refine the supported annotation to ubiquitin protein ligase binding. Reason: The literal PMID:33961781 interaction records identify RNF111 (Q6ZNA4) using anti tag coimmunoprecipitation. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The named RNF2/RNF111 partner is an established ubiquitin E3 ligase. The term records partner class, not a claim that CK2 ubiquitinates substrates or that every recovered complex represents a direct catalytic-site contact. Proposed replacements: ubiquitin protein ligase binding |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:33961781 interaction records identify FAM133A (Q8N9E0) using anti tag coimmunoprecipitation. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:33961781 interaction records identify DVL3 (Q92997) using anti tag coimmunoprecipitation. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | MODIFY | Summary: Refine the supported annotation to ubiquitin protein ligase binding. Reason: The literal PMID:33961781 interaction records identify RNF2 (Q99496) using anti tag coimmunoprecipitation. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. The named RNF2/RNF111 partner is an established ubiquitin E3 ligase. The term records partner class, not a claim that CK2 ubiquitinates substrates or that every recovered complex represents a direct catalytic-site contact. Proposed replacements: ubiquitin protein ligase binding |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:33961781 interaction records identify TSPYL2 (Q9H2G4) using anti tag coimmunoprecipitation. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:34316702 Elucidation of the BMI1 interactome identifies novel regulat... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:34316702 interaction records identify BMI1 (P35226) using anti bait coimmunoprecipitation. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The authentic OpenCell source supports co-enrichment with AIP (O00170), with CSNK2A1/CSNK2A3 peptide-group ambiguity retained. This is association evidence, not purified binary affinity or an alpha 1-specific native stoichiometry. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The authentic OpenCell source supports co-enrichment with EIF3J (O75822), with CSNK2A1/CSNK2A3 peptide-group ambiguity retained. This is association evidence, not purified binary affinity or an alpha 1-specific native stoichiometry. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The authentic OpenCell source supports co-enrichment with RPLP1 (P05386), with CSNK2A1/CSNK2A3 peptide-group ambiguity retained. This is association evidence, not purified binary affinity or an alpha 1-specific native stoichiometry. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The authentic OpenCell source supports co-enrichment with HSP90AB1 (P08238), with CSNK2A1/CSNK2A3 peptide-group ambiguity retained. This is association evidence, not purified binary affinity or an alpha 1-specific native stoichiometry. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The authentic OpenCell source supports co-enrichment with NCL (P19338), with CSNK2A1/CSNK2A3 peptide-group ambiguity retained. This is association evidence, not purified binary affinity or an alpha 1-specific native stoichiometry. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The authentic OpenCell source supports co-enrichment with CSNK2A2 (P19784), with CSNK2A1/CSNK2A3 peptide-group ambiguity retained. This is association evidence, not purified binary affinity or an alpha 1-specific native stoichiometry. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The authentic OpenCell source supports co-enrichment with EIF2S2 (P20042), with CSNK2A1/CSNK2A3 peptide-group ambiguity retained. This is association evidence, not purified binary affinity or an alpha 1-specific native stoichiometry. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The authentic OpenCell source supports co-enrichment with RPS3 (P23396), with CSNK2A1/CSNK2A3 peptide-group ambiguity retained. This is association evidence, not purified binary affinity or an alpha 1-specific native stoichiometry. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The authentic OpenCell source supports co-enrichment with DEK (P35659), with CSNK2A1/CSNK2A3 peptide-group ambiguity retained. This is association evidence, not purified binary affinity or an alpha 1-specific native stoichiometry. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The authentic OpenCell source supports co-enrichment with RPS18 (P62269), with CSNK2A1/CSNK2A3 peptide-group ambiguity retained. This is association evidence, not purified binary affinity or an alpha 1-specific native stoichiometry. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The authentic OpenCell source supports co-enrichment with CSNK2B (P67870), with CSNK2A1/CSNK2A3 peptide-group ambiguity retained. This is association evidence, not purified binary affinity or an alpha 1-specific native stoichiometry. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The authentic OpenCell source supports co-enrichment with SET (Q01105), with CSNK2A1/CSNK2A3 peptide-group ambiguity retained. This is association evidence, not purified binary affinity or an alpha 1-specific native stoichiometry. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The authentic OpenCell source supports co-enrichment with SSRP1 (Q08945), with CSNK2A1/CSNK2A3 peptide-group ambiguity retained. This is association evidence, not purified binary affinity or an alpha 1-specific native stoichiometry. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The authentic OpenCell source supports co-enrichment with SF3B2 (Q13435), with CSNK2A1/CSNK2A3 peptide-group ambiguity retained. This is association evidence, not purified binary affinity or an alpha 1-specific native stoichiometry. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The authentic OpenCell source supports co-enrichment with HDAC1 (Q13547), with CSNK2A1/CSNK2A3 peptide-group ambiguity retained. This is association evidence, not purified binary affinity or an alpha 1-specific native stoichiometry. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The authentic OpenCell source supports co-enrichment with HDAC2 (Q92769), with CSNK2A1/CSNK2A3 peptide-group ambiguity retained. This is association evidence, not purified binary affinity or an alpha 1-specific native stoichiometry. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The authentic OpenCell source supports co-enrichment with LTV1 (Q96GA3), with CSNK2A1/CSNK2A3 peptide-group ambiguity retained. This is association evidence, not purified binary affinity or an alpha 1-specific native stoichiometry. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The authentic OpenCell source supports co-enrichment with RNF2 (Q99496), with CSNK2A1/CSNK2A3 peptide-group ambiguity retained. This is association evidence, not purified binary affinity or an alpha 1-specific native stoichiometry. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The authentic OpenCell source supports co-enrichment with DDX24 (Q9GZR7), with CSNK2A1/CSNK2A3 peptide-group ambiguity retained. This is association evidence, not purified binary affinity or an alpha 1-specific native stoichiometry. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The authentic OpenCell source supports co-enrichment with MAF1 (Q9H063), with CSNK2A1/CSNK2A3 peptide-group ambiguity retained. This is association evidence, not purified binary affinity or an alpha 1-specific native stoichiometry. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The authentic OpenCell source supports co-enrichment with SUDS3 (Q9H7L9), with CSNK2A1/CSNK2A3 peptide-group ambiguity retained. This is association evidence, not purified binary affinity or an alpha 1-specific native stoichiometry. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. |
| GO:0005515 protein binding | IPI PMID:36833176 Haploinsufficiency as a Foreground Pathomechanism of Poirer-... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:36833176 interaction records identify CSNK2B (P67870) using anti tag coimmunoprecipitation. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. The source records beta mutations/truncation and HA tagging; assembly conclusions are limited to the tested constructs. |
| GO:0005515 protein binding | IPI PMID:38225382 Systematic discovery of protein interaction interfaces using... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The literal PMID:38225382 interaction records identify CSNK2B (P67870) using bioluminescence resonance energy transfer. Preserve the actual construct and assay scope; tagged or complex-based evidence is not silently promoted to native direct binding. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. This source includes an actual BRET experiment despite its structure-prediction focus; it is not treated as prediction-only evidence. |
| GO:0005515 protein binding | IPI PMID:40205054 Multimodal cell maps as a foundation for structural and func... | KEEP AS NON CORE | Summary: Retain the supported protein binding context as non-core. Reason: The original PMID:40205054 assertion identifies CSNK2B (P67870). No exact pair was recovered in the current MITAB snapshot; that access difference is not evidence that the curator-supported positive interaction is absent. Retain the supported interaction as non-core under the project binding policy; the available assay does not establish a more informative target molecular activity. The positive assembly-focused source is preserved even though a matching pair is absent from the current interaction feed. |
| GO:0005524 ATP binding | IEA GO_REF:0000002 | ACCEPT | Summary: Retain the supported ATP binding annotation. Reason: ATP binding is a genuine component of CK2 catalysis and agrees with the official canonical ATP-binding features. The 255-aa product deletes these annotated sites; this source is retained as a gene-product-level annotation, without asserting that every numbered product binds ATP. Propagation Review Root cause: NO FAILURE CORE Sources checked: InterPro:IPR000719 SUPPORTS TRANSFER The actual kinase domains and canonical ATP-site features support ATP binding; product 2 equivalence is not presumed. InterPro:IPR017441 SUPPORTS TRANSFER The actual kinase domains and canonical ATP-site features support ATP binding; product 2 equivalence is not presumed. |
| GO:0005634 nucleus | EXP PMID:24962073 CK2Ξ± phosphorylates DBC1 and is involved in the progression ... | ACCEPT | Summary: Retain the supported nucleus annotation. Reason: The source studies CK2 alpha and DBC1 in human gastric carcinomas and cancer cells, reporting immunofluorescence alongside association and kinase assays. Its detailed nuclear-localization experiment was not independently recovered; nuclear CK2 alpha is corroborated by direct localization work and current HPA nucleoplasm data, so retain the experimental location with curator deference. |
| GO:0005634 nucleus | HDA PMID:21630459 Proteomic characterization of the human sperm nucleus. | ACCEPT | Summary: Retain the supported nucleus annotation. Reason: The cited human sperm-nuclear proteomics study supports a nuclear protein pool. The complete abstract was read, but the target-specific table was not inspected; independently observed human nuclear CK2 alpha supports retention without inferring sperm-specific activity. |
| GO:0005634 nucleus | IBA GO_REF:0000033 | ACCEPT | Summary: Retain the supported nucleus annotation. Reason: The actual GO:0005634 IBD at PTN000621946 lies on the verified human target path in the current 540-node family. Human experimental nuclear localization is also present among the descendant evidence. Target-self evidence is legitimate grounding, not circularity. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000621946 SUPPORTS TRANSFER The exact term IBD at this proximate ancestor is on the verified target path. Extant source records and sequence identity are retained separately; no donor-count or self-circularity objection applies. |
| GO:0005634 nucleus | IDA PMID:21282530 Interaction proteomics analysis of polycomb proteins defines... | ACCEPT | Summary: Retain the supported nucleus annotation. Reason: The original human-cell CBX study identifies nuclear CK2 alpha-containing assemblies and distinguishes alpha association from direct CBX binding through beta. The nuclear location is supported without equating it with exclusive Polycomb-focus localization. |
| GO:0005634 nucleus | IDA PMID:23123191 Functional interaction of protein kinase CK2 and activating ... | ACCEPT | Summary: Retain the supported nucleus annotation. Reason: The PMID:23123191 abstract explicitly places CK2βATF4 bimolecular fluorescence complementation in nuclei. Retain the nuclear location, with full original Methods unavailable and no inference of an exclusive nuclear pool. |
| GO:0005634 nucleus | IEA GO_REF:0000044 | ACCEPT | Summary: Retain the supported nucleus annotation. Reason: The original SL-0191 UniProt localization mapping is concordant with independently observed nuclear CK2 alpha and HPA staining. A broad nuclear assertion is valid even though nucleoplasm is documented for particular assays. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB-SubCell:SL-0191 SUPPORTS TRANSFER The official SL-0191 nuclear location is corroborated by HPA and original localization evidence. |
| GO:0005634 nucleus | TAS PMID:10799509 Identification and characterization of CKIP-1, a novel pleck... | ACCEPT | Summary: Retain the supported nucleus annotation. Reason: The original indexed CKIP-1 paper describes endogenous CK2 as predominantly nuclear and tests redistribution in transfected cells. Preserve this nuclear TAS without converting the inconclusive membrane codistribution image into exclusive localization. |
| GO:0005654 nucleoplasm | IDA GO_REF:0000052 | ACCEPT | Summary: Retain the supported nucleoplasm annotation. Reason: Current HPA ICC/IF records nucleoplasm for CAB020680 in A-431/U-251MG and HPA059206 in A-431/Hep-G2/U2OS/KOLF2.1J. The XML and antibody/cell-line metadata were read; no independent image-pixel assessment or new cilium annotation is claimed. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6805059 | ACCEPT | Summary: Retain the supported nucleoplasm annotation. Reason: The actual R-HSA-6805059 event models CK2:FACT phosphorylation of TP53 at Ser392. Its catalyst/component/member path reaches UniProt P68400 in nucleoplasm, including the alpha-containing CK2 alternative where applicable. Retain this event-specific location; it is not a claim that every underlying experiment used isolated human alpha or that all cellular CK2 occupies this compartment. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6805061 | ACCEPT | Summary: Retain the supported nucleoplasm annotation. Reason: The actual R-HSA-6805061 event models UV-associated CK2βFACT assembly. Its catalyst/component/member path reaches UniProt P68400 in nucleoplasm, including the alpha-containing CK2 alternative where applicable. Retain this event-specific location; it is not a claim that every underlying experiment used isolated human alpha or that all cellular CK2 occupies this compartment. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9831712 | ACCEPT | Summary: Retain the supported nucleoplasm annotation. Reason: The actual R-HSA-9831712 event models respiratory-syncytial-virus matrix-protein phosphorylation. Its catalyst/component/member path reaches UniProt P68400 in nucleoplasm, including the alpha-containing CK2 alternative where applicable. Retain this event-specific location; it is not a claim that every underlying experiment used isolated human alpha or that all cellular CK2 occupies this compartment. |
| GO:0005829 cytosol | IBA GO_REF:0000033 | ACCEPT | Summary: Retain the supported cytosol annotation. Reason: The actual cytosol IBD at PTN000621946 lies on the human target path and is supported by experimentally annotated descendants. Independent current Reactome component paths explicitly place alpha-containing CK2 in cytosol; no absent-node or donor-count objection applies. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000621946 SUPPORTS TRANSFER The exact term IBD at this proximate ancestor is on the verified target path. Extant source records and sequence identity are retained separately; no donor-count or self-circularity objection applies. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-201717 | ACCEPT | Summary: Retain the supported cytosol annotation. Reason: The actual R-HSA-201717 event models DVL phosphorylation. Its catalyst/component/member path reaches UniProt P68400 in cytosol, including the alpha-containing CK2 alternative where applicable. Retain this event-specific location; it is not a claim that every underlying experiment used isolated human alpha or that all cellular CK2 occupies this compartment. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-2529020 | ACCEPT | Summary: Retain the supported cytosol annotation. Reason: The actual R-HSA-2529020 event models condensin-I phosphorylation. Its catalyst/component/member path reaches UniProt P68400 in cytosol, including the alpha-containing CK2 alternative where applicable. Retain this event-specific location; it is not a claim that every underlying experiment used isolated human alpha or that all cellular CK2 occupies this compartment. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-392752 | ACCEPT | Summary: Retain the supported cytosol annotation. Reason: The actual R-HSA-392752 event models L1CAM phosphorylation. Its catalyst/component/member path reaches UniProt P68400 in cytosol, including the alpha-containing CK2 alternative where applicable. Retain this event-specific location; it is not a claim that every underlying experiment used isolated human alpha or that all cellular CK2 occupies this compartment. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-6814409 | ACCEPT | Summary: Retain the supported cytosol annotation. Reason: The actual R-HSA-6814409 event models PDCL phosphorylation. Its catalyst/component/member path reaches UniProt P68400 in cytosol, including the alpha-containing CK2 alternative where applicable. Retain this event-specific location; it is not a claim that every underlying experiment used isolated human alpha or that all cellular CK2 occupies this compartment. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8850945 | ACCEPT | Summary: Retain the supported cytosol annotation. Reason: The actual R-HSA-8850945 event models PTEN phosphorylation. Its catalyst/component/member path reaches UniProt P68400 in cytosol, including the alpha-containing CK2 alternative where applicable. Retain this event-specific location; it is not a claim that every underlying experiment used isolated human alpha or that all cellular CK2 occupies this compartment. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8873929 | ACCEPT | Summary: Retain the supported cytosol annotation. Reason: The actual R-HSA-8873929 event models STARD10 phosphorylation. Its catalyst/component/member path reaches UniProt P68400 in cytosol, including the alpha-containing CK2 alternative where applicable. Retain this event-specific location; it is not a claim that every underlying experiment used isolated human alpha or that all cellular CK2 occupies this compartment. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8948039 | ACCEPT | Summary: Retain the supported cytosol annotation. Reason: The actual R-HSA-8948039 event models FUNDC1 phosphorylation. Its catalyst/component/member path reaches UniProt P68400 in cytosol, including the alpha-containing CK2 alternative where applicable. Retain this event-specific location; it is not a claim that every underlying experiment used isolated human alpha or that all cellular CK2 occupies this compartment. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9831514 | ACCEPT | Summary: Retain the supported cytosol annotation. Reason: The actual R-HSA-9831514 event models respiratory-syncytial-virus P-protein phosphorylation. Its catalyst/component/member path reaches UniProt P68400 in cytosol, including the alpha-containing CK2 alternative where applicable. Retain this event-specific location; it is not a claim that every underlying experiment used isolated human alpha or that all cellular CK2 occupies this compartment. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9931418 | ACCEPT | Summary: Retain the supported cytosol annotation. Reason: The actual R-HSA-9931418 event models CD274 phosphorylation. Its catalyst/component/member path reaches UniProt P68400 in cytosol, including the alpha-containing CK2 alternative where applicable. Retain this event-specific location; it is not a claim that every underlying experiment used isolated human alpha or that all cellular CK2 occupies this compartment. |
| GO:0005886 plasma membrane | TAS PMID:10799509 Identification and characterization of CKIP-1, a novel pleck... | KEEP AS NON CORE | Summary: Retain the supported plasma membrane context as non-core. Reason: The original CKIP-1 study discusses prior peripheral membrane CK2 and tests a membrane-localized CKIP-1 interactor, but explicitly finds CK2 codistribution difficult to resolve. Retain the original TAS as a context-dependent membrane-associated pool; do not infer an integral membrane kinase or claim decisive membrane imaging from this experiment. |
| GO:0005956 protein kinase CK2 complex | IBA GO_REF:0000033 | ACCEPT | Summary: Retain the supported protein kinase CK2 complex annotation. Reason: The actual CK2-complex IBD at PTN000621946 lies on the verified human target path. Human alpha/beta structural evidence independently corroborates membership, while free-alpha catalysis and shorter-product assembly remain separate questions. Neither donor count nor legitimate target-self support is a failure. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000621946 SUPPORTS TRANSFER The exact term IBD at this proximate ancestor is on the verified target path. Extant source records and sequence identity are retained separately; no donor-count or self-circularity objection applies. |
| GO:0005956 protein kinase CK2 complex | IDA PMID:15723517 Interactions of the HIV-1 Tat and RAP74 proteins with the RN... | ACCEPT | Summary: Retain the supported protein kinase CK2 complex annotation. Reason: CK2 complex membership is well established for this gene. PMID:15723517 remains abstract-only at the selected original-access boundary, so its precise complex assay is retained with curator deference rather than asserted to have been independently inspected or rejected from its FCP1-focused title. |
| GO:0005956 protein kinase CK2 complex | IPI PMID:22506723 Structural determinants of protein kinase CK2 regulation by ... | ACCEPT | Summary: Retain the supported protein kinase CK2 complex annotation. Reason: PMID:22506723 reports the CK2 holoenzyme crystal structure. Its primary deposition 4DGL directly identifies two human CSNK2A1 and two CSNK2B chains, supporting this complex assertion. The alpha construct is 335 aa and the deposition sequence/declared mutation disagree at 125; retain those construct limits and do not claim this establishes universal physiological polymerization. |
| GO:0006302 double-strand break repair | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: Retain the supported double-strand break repair context as non-core. Reason: The actual GO:0006302 IBD at PTN002779318 is on the human path, with human CSNK2A1 itself among the experimental sources. Direct RAD51/MDC1 phosphorylation provides performed repair-regulatory work. Retain this positive pathway role as non-core; self-grounding and a recent ancestor are not propagation failures. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: PANTHER:PTN002779318 SUPPORTS TRANSFER The exact term IBD at this proximate ancestor is on the verified target path. Extant source records and sequence identity are retained separately; no donor-count or self-circularity objection applies. |
| GO:0006302 double-strand break repair | IDA PMID:22325354 Plk1 and CK2 act in concert to regulate Rad51 during DNA dou... | KEEP AS NON CORE | Summary: Retain the supported double-strand break repair context as non-core. Reason: The original CK2/PLK1 RAD51 experiments demonstrate phosphorylation-dependent recruitment and repair effects. CK2 performs a regulatory step in this repair context; retain the process as non-core rather than turning every substrate-specific pathway into a separate catalytic core. |
| GO:0006457 protein folding | TAS PMID:19387550 Protein kinase CK2 in health and disease: CK2: the kinase co... | KEEP AS NON CORE | Summary: Retain the supported protein folding context as non-core. Reason: The cited review abstract describes CK2 regulation of chaperone systems, and independently read FNIP1/DNAJB2 work gives concrete phosphorylation-dependent effects. GO:0006457 includes assistance in protein/complex assembly; preserve this contextual role without assigning intrinsic chaperone activity to CK2. |
| GO:0006974 DNA damage response | IDA PMID:18411307 Phosphorylation of SDT repeats in the MDC1 N terminus trigge... | KEEP AS NON CORE | Summary: Retain the supported DNA damage response context as non-core. Reason: The original MDC1 SDTD-repeat experiments combine CK2-dependent phosphorylation, phosphopeptide recognition and NBS1/MRN recruitment. The performed kinase step is distinct from the downstream DNA-repair machinery. Retain the performed DNA-damage-response contribution as a context-specific pathway role rather than a distinct molecular core. |
| GO:0006974 DNA damage response | IDA PMID:18583988 Phospho-dependent interactions between NBS1 and MDC1 mediate... | KEEP AS NON CORE | Summary: Retain the supported DNA damage response context as non-core. Reason: CK2-dependent phosphorylation of MDC1 SDTD repeats permits NBS1/MRN association. The read main Methods and Results support CK2 chemistry, without claiming that every assay isolates the alpha paralog or that all supplementary experiments were inspected. Retain the performed DNA-damage-response contribution as a context-specific pathway role rather than a distinct molecular core. |
| GO:0006974 DNA damage response | IDA PMID:20545769 Casein kinase 2-dependent phosphorylation of human Rad9 medi... | KEEP AS NON CORE | Summary: Retain the supported DNA damage response context as non-core. Reason: The complete abstract reports CK2 phosphorylation of RAD9 Ser387 and recruitment of TOPBP1; the current GO-CAM explicitly models the CK2 reaction on RAD9. Retain the sound catalytic assertion with curator deference because detailed original assay conditions were not recovered. Retain the performed DNA-damage-response contribution as a context-specific pathway role rather than a distinct molecular core. |
| GO:0006974 DNA damage response | IDA PMID:30898438 MDC1 Interacts with TOPBP1 to Maintain Chromosomal Stability... | KEEP AS NON CORE | Summary: Retain the supported DNA damage response context as non-core. Reason: The original tests constitutive CK2-dependent MDC1 Ser168/Ser196 phosphorylation and TOPBP1 recruitment during mitosis. The phosphorylation is not described as newly induced by DNA damage. Retain the performed DNA-damage-response contribution as a context-specific pathway role rather than a distinct molecular core. |
| GO:0006974 DNA damage response | IDA PMID:35597237 A PARylation-phosphorylation cascade promotes TOPBP1 loading... | KEEP AS NON CORE | Summary: Retain the supported DNA damage response context as non-core. Reason: The complete abstract reports CK2 phosphorylation of HTATSF1 Ser748 and TOPBP1-dependent RPA-to-RAD51 exchange; the authentic GO-CAM records this performed reaction. Full primary assay methods were not recovered. Retain the performed DNA-damage-response contribution as a context-specific pathway role rather than a distinct molecular core. |
| GO:0007165 signal transduction | TAS PMID:2174700 Isolation and characterization of human cDNA clones encoding... | KEEP AS NON CORE | Summary: Retain the supported signal transduction context as non-core. Reason: The broad signaling role is biologically coherent with direct phosphorylation of signaling proteins, including the independently read Wnt and PTEN contexts. The original PMID:2174700 access is limited to a cDNA/sequence abstract; retain its TAS as broad non-core context without attributing a pathway perturbation to that unread full paper. |
| GO:0008284 positive regulation of cell population proliferation | IDA PMID:20625391 Functional polymorphism of the CK2alpha intronless gene play... | KEEP AS NON CORE | Summary: Retain the supported positive regulation of cell population proliferation context as non-core. Reason: The original compares authentic CSNK2A1 and CSNK2A1P in proliferation assays, including kinase controls. Positive growth regulation by the annotated target is retained at the tested overexpression context, including mouse NIH3T3 colony and soft-agar assays; the related-gene title does not justify exclusion. |
| GO:0016301 kinase activity | IDA PMID:18548200 Transcription factor TAFII250 phosphorylates the acidic doma... | MODIFY | Summary: Refine the supported annotation to protein serine/threonine kinase activity. Reason: The actual PMID:18548200 study detects human CK2 alpha in reciprocal TAFII250 co-immunoprecipitates and a TBB-sensitive Mdm2 kinase activity. Broad kinase activity can refine to the established target Ser/Thr class. The paper does not resolve the exact Mdm2 phosphosite in these complexes and also permits a residual non-CK2 activity. Proposed replacements: protein serine/threonine kinase activity Supporting Evidence: PMID:18548200 CK2 is tightly associated with TAFII250 |
| GO:0016605 PML body | IDA PMID:20719947 Epstein-Barr virus nuclear antigen 1 Hijacks the host kinase... | KEEP AS NON CORE | Summary: Retain the supported PML body context as non-core. Reason: The original EBNA1 study identifies PML-associated CK2 and shows CK2-alpha depletion affecting PML-body disruption, but states that pannuclear alpha/beta staining complicates their focal localization; quantified recruitment emphasizes beta. Retain the curator-supported PML-associated context without claiming direct alphaβPML contact or exclusive residence in PML bodies. |
| GO:0017148 negative regulation of translation | IDA PMID:30765518 Phosphoregulated FMRP phase separation models activity-depen... | KEEP AS NON CORE | Summary: Retain the supported negative regulation of translation context as non-core. Reason: The original uses CK2 phosphorylation of recombinant FMRP low-complexity fragments before condensate and translation assays, with CK2 subsequently removed. This directly supports kinase chemistry but is not an endogenous human neuronal CK2 knockout experiment. Preserve the phosphorylation-dependent translation effect as a reconstituted, substrate-specific non-core role, not a universal repression mechanism. |
| GO:0017148 negative regulation of translation | IDA PMID:31439799 Phospho-dependent phase separation of FMRP and CAPRIN1 recap... | KEEP AS NON CORE | Summary: Retain the supported negative regulation of translation context as non-core. Reason: The recovered author paper assays phosphorylated FMRP/CAPRIN1 low-complexity constructs, phase separation, reticulocyte translation and deadenylation. The kinase treatment is positive, with reconstituted substrate and heterologous translation contexts retained. Preserve the phosphorylation-dependent translation effect as a reconstituted, substrate-specific non-core role, not a universal repression mechanism. |
| GO:0018105 peptidyl-serine phosphorylation | IDA PMID:24657168 Inositol pyrophosphates mediate the DNA-PK/ATM-p53 cell deat... | ACCEPT | Summary: Retain the supported peptidyl-serine phosphorylation annotation. Reason: The source reports serine phosphorylation of the TTT machinery, a performed reaction compatible with CK2 chemistry. Preserve its source-specific Ser process while noting the original main-text alpha/alpha-prime construct naming ambiguity and the later corrected panels; separate human alpha studies independently establish this chemistry. |
| GO:0030177 positive regulation of Wnt signaling pathway | IMP PMID:12700239 CK2 phosphorylation of the armadillo repeat region of beta-c... | KEEP AS NON CORE | Summary: Retain the supported positive regulation of Wnt signaling pathway context as non-core. Reason: The full original PMID:12700239 supports positive Wnt regulation through beta-catenin phosphorylation and altered reporter output. Preserve this contextual signaling role with the joint alpha/alpha-prime inhibition and separate Xenopus substrate-stability assays explicit; it need not become an additional molecular core. Supporting Evidence: PMID:12700239 CK2 is a positive regulator of Wnt signaling |
| GO:0030307 positive regulation of cell growth | IDA PMID:20625391 Functional polymorphism of the CK2alpha intronless gene play... | KEEP AS NON CORE | Summary: Retain the supported positive regulation of cell growth context as non-core. Reason: The original includes authentic CSNK2A1 as well as the related CSNK2A1P product in growth experiments. Retain the observed positive growth context without conflating the separate loci or claiming every physiological setting has this outcome. |
| GO:0031507 heterochromatin formation | NAS PMID:22325352 PCGF homologs, CBX proteins, and RYBP define functionally di... | UNDECIDED | Summary: The source-specific heterochromatin formation assertion remains unresolved. Reason: The current definition of GO:0031507 describes heterochromatin nucleation, spreading and boundary formation. The cited PRC1 main text was read, but its CSNK2A1-specific supplemental provenance was not independently located and the recovered CK2βPolycomb assays do not directly resolve this process. Retain uncertainty for this NAS assertion rather than declaring the gene incapable of heterochromatin regulation. |
| GO:0031519 PcG protein complex | IDA PMID:21282530 Interaction proteomics analysis of polycomb proteins defines... | KEEP AS NON CORE | Summary: Retain the supported PcG protein complex context as non-core. Reason: The actual definition is PcG protein complex, not a nuclear body. Human CBX affinity purifications and CK2-alpha GST pulldowns from nuclear extracts support a PcG-associated pool; direct CBX4 binding is demonstrated for beta, not alpha. Retain non-core complex membership with this assembly limit, not a false negative based on focal imaging. |
| GO:0032435 negative regulation of proteasomal ubiquitin-dependent protein catabolic process | IGI PMID:28031292 Protein kinase CK2 modulates HSJ1 function through phosphory... | KEEP AS NON CORE | Summary: Retain the supported negative regulation of proteasomal ubiquitin-dependent protein catabolic process context as non-core. Reason: The actual HSJ1/DNAJB2 study shows CK2-dependent phosphorylation reduces ubiquitin-client binding and degradation, with phosphorylation-site controls. Preserve this measured negative proteasomal-regulation context without giving CK2 an intrinsic ubiquitin-reader or chaperone activity. Supporting Evidence: PMID:28031292 phosphorylates HSJ1 within its second UIM |
| GO:0035102 PRC1 complex | NAS PMID:22325352 PCGF homologs, CBX proteins, and RYBP define functionally di... | KEEP AS NON CORE | Summary: Retain the supported PRC1 complex context as non-core. Reason: The cited PRC1 study provides the original complex assertion, with target-specific supplemental coverage uninspected. Independent human CBX and AUTS2βPRC1.5 studies demonstrate CK2-associated pools. Retain contextual membership; the AUTS2 complex has altered ubiquitination/transcriptional behavior and is not evidence for universal canonical PRC1 repression. |
| GO:0042176 regulation of protein catabolic process | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Retain the supported regulation of protein catabolic process context as non-core. Reason: The current ARBA00027460 rule has one verified matching branch: FunFams 1.10.510.10:FF:000059 and 3.30.200.20:FF:000088 with Haplorrhini. Its literal output is this broad regulation term. Substrate-specific studies show both stabilization and degradation effects, so preserve positive context-dependent regulation without alleging a rule mismatch. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: ARBA:ARBA00027460 SUPPORTS TRANSFER Actual ARBA00027460 branch 4 matches both official FunFams and Haplorrhini, with literal output GO:0042176; retain contextual regulation. |
| GO:0042802 identical protein binding | IPI PMID:24013921 The CK2 kinase stabilizes CLOCK and represses its activity i... | UNDECIDED | Summary: The source-specific identical protein binding assertion remains unresolved. Reason: The original circadian study uses Drosophila CLK/cellular/animal contexts and commercial human CK2 alpha in kinase assays. The read main text does not resolve a target-specific alphaβalpha binding experiment; uninspected supplementary scope prevents a confident rejection. Keep this citation-specific identical-binding assertion undecided, distinct from the independently positive human screening assertion. |
| GO:0042802 identical protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the supported identical protein binding context as non-core. Reason: The actual HuRI record supports a positive tagged human CK2-alpha self-pair and both participant sequences reconstruct exactly. Retain identical-protein binding as non-core; this screen does not by itself establish native homodimer stoichiometry or exclude beta-mediated cellular assembly. |
| GO:0042802 identical protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | KEEP AS NON CORE | Summary: Retain the supported identical protein binding context as non-core. Reason: The authentic OpenCell dataset contains the target in grouped CSNK2A1/CSNK2A3 prey assignments. Preserve the positive co-enrichment context as non-core, while peptide-group ambiguity prevents a clean alpha 1-only homomer claim or native stoichiometry inference. |
| GO:0045732 positive regulation of protein catabolic process | IDA PMID:20625391 Functional polymorphism of the CK2alpha intronless gene play... | KEEP AS NON CORE | Summary: Retain the supported positive regulation of protein catabolic process context as non-core. Reason: The original authentic CSNK2A1 control participates in PML phosphorylation/degradation experiments alongside CSNK2A1P. Retain the positive substrate-specific protein-catabolic effect as non-core without generalizing it to all CK2 substrates. |
| GO:0045893 positive regulation of DNA-templated transcription | NAS PMID:22325352 PCGF homologs, CBX proteins, and RYBP define functionally di... | KEEP AS NON CORE | Summary: Retain the supported positive regulation of DNA-templated transcription context as non-core. Reason: The cited PRC1 proteomic source alone does not resolve the target transcription assay in the main text read. Independent AUTS2βPRC1.5 work shows CK2-dependent RING1B phosphorylation and reduced H2A ubiquitination in an activating complex, with P300 recruitment a separate AUTS2 contribution. Preserve the contextual positive transcription role without attributing P300 activity or an autonomous transcriptional activator MF to CK2. |
| GO:0050821 protein stabilization | IDA PMID:22184066 Phosphorylation of the transcription factor YY1 by CK2Ξ± prev... | KEEP AS NON CORE | Summary: Retain the supported protein stabilization context as non-core. Reason: The complete PMID:22184066 abstract and current GO-CAM support CK2 phosphorylation protecting YY1 from caspase-7 cleavage. Retain the performed substrate-stabilization role as non-core, with detailed original assay conditions unavailable and without implying that CK2 stabilizes every substrate. |
| GO:0051726 regulation of cell cycle | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: Retain the supported regulation of cell cycle context as non-core. Reason: The actual cell-cycle IBD at PTN000621946 is inherited by the verified human target and has experimentally annotated descendants. The human checkpoint literature independently supports a regulatory role. Retain it as broad context-dependent non-core biology; an extant target lacking an exact current experimental row is not a phylogenetic failure. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: PANTHER:PTN000621946 SUPPORTS TRANSFER The exact term IBD at this proximate ancestor is on the verified target path. Extant source records and sequence identity are retained separately; no donor-count or self-circularity objection applies. |
| GO:0051879 Hsp90 protein binding | TAS PMID:19387550 Protein kinase CK2 in health and disease: CK2: the kinase co... | KEEP AS NON CORE | Summary: Retain the supported Hsp90 protein binding context as non-core. Reason: The cited chaperone review remains abstract-only, but the positive Hsp90-associated CK2 interaction is independently corroborated by the actual interaction records. Retain specific Hsp90 binding as non-core without assigning CK2 the folding activity of its partner. |
| GO:0070822 Sin3-type complex | IDA PMID:17827154 Differential distribution of unmodified and phosphorylated h... | KEEP AS NON CORE | Summary: Retain the supported Sin3-type complex context as non-core. Reason: The original HDAC2 study reports CK2 in human Sin3/Mi2-associated immunoprecipitates and reciprocal alpha-directed experiments. This supports a Sin3-associated regulatory pool, not universal stoichiometric membership in every Sin3 complex or direct contact with each component. |
| GO:0075342 symbiont-mediated disruption of host cell PML body | IDA PMID:20719947 Epstein-Barr virus nuclear antigen 1 Hijacks the host kinase... | KEEP AS NON CORE | Summary: Retain the supported symbiont-mediated disruption of host cell PML body context as non-core. Reason: The original human EBV study shows alpha depletion and CK2-dependent PML phosphorylation/degradation in EBNA1-mediated PML-body disruption. CK2 performs a host kinase step exploited by the virus. Retain this positive infection-specific process as non-core; direct EBNA1 binding is to beta and is not silently assigned to alpha. |
| GO:0106310 protein serine kinase activity | EXP PMID:18411307 Phosphorylation of SDT repeats in the MDC1 N terminus trigge... | MODIFY | Summary: Refine the supported annotation to protein serine/threonine kinase activity. Reason: The original MDC1 SDTD-repeat experiments combine CK2-dependent phosphorylation, phosphopeptide recognition and NBS1/MRN recruitment. The performed kinase step is distinct from the downstream DNA-repair machinery. The source-specific serine phosphorylation remains positive. The GO enzyme-class convention documented in issue 20114 (https://github.com/geneontology/go-ontology/issues/20114#issuecomment-909198642) places established Ser/Thr kinases such as CK2 in GO:0004674, while retaining GO:0106310 for the exceptional serine-specific class. This is class scoping, not rejection of the residue assay or an is_a generalization. The EXP source remains distinct from the same-PMID GO:0004674 IDA row; both original source objects are preserved. Proposed replacements: protein serine/threonine kinase activity |
| GO:0106310 protein serine kinase activity | EXP PMID:18583988 Phospho-dependent interactions between NBS1 and MDC1 mediate... | MODIFY | Summary: Refine the supported annotation to protein serine/threonine kinase activity. Reason: CK2-dependent phosphorylation of MDC1 SDTD repeats permits NBS1/MRN association. The read main Methods and Results support CK2 chemistry, without claiming that every assay isolates the alpha paralog or that all supplementary experiments were inspected. The source-specific serine phosphorylation remains positive. The GO enzyme-class convention documented in issue 20114 (https://github.com/geneontology/go-ontology/issues/20114#issuecomment-909198642) places established Ser/Thr kinases such as CK2 in GO:0004674, while retaining GO:0106310 for the exceptional serine-specific class. This is class scoping, not rejection of the residue assay or an is_a generalization. The EXP source remains distinct from the same-PMID GO:0004674 IDA row; both original source objects are preserved. Proposed replacements: protein serine/threonine kinase activity |
| GO:0106310 protein serine kinase activity | EXP PMID:18678890 MDC1 regulates intra-S-phase checkpoint by targeting NBS1 to... | MODIFY | Summary: Refine the supported annotation to protein serine/threonine kinase activity. Reason: MDC1 phosphosite mass spectrometry, inhibition and phosphomimetic/binding experiments support the established CK2 catalytic class. The read original main text does not itself supply a newly performed recombinant CK2 assay; that distinction does not invalidate the curator-supported activity. The source-specific serine phosphorylation remains positive. The GO enzyme-class convention documented in issue 20114 (https://github.com/geneontology/go-ontology/issues/20114#issuecomment-909198642) places established Ser/Thr kinases such as CK2 in GO:0004674, while retaining GO:0106310 for the exceptional serine-specific class. This is class scoping, not rejection of the residue assay or an is_a generalization. The EXP source remains distinct from the same-PMID GO:0004674 IDA row; both original source objects are preserved. Proposed replacements: protein serine/threonine kinase activity |
| GO:0106310 protein serine kinase activity | EXP PMID:20545769 Casein kinase 2-dependent phosphorylation of human Rad9 medi... | MODIFY | Summary: Refine the supported annotation to protein serine/threonine kinase activity. Reason: The complete abstract reports CK2 phosphorylation of RAD9 Ser387 and recruitment of TOPBP1; the current GO-CAM explicitly models the CK2 reaction on RAD9. Retain the sound catalytic assertion with curator deference because detailed original assay conditions were not recovered. The source-specific serine phosphorylation remains positive. The GO enzyme-class convention documented in issue 20114 (https://github.com/geneontology/go-ontology/issues/20114#issuecomment-909198642) places established Ser/Thr kinases such as CK2 in GO:0004674, while retaining GO:0106310 for the exceptional serine-specific class. This is class scoping, not rejection of the residue assay or an is_a generalization. The EXP source remains distinct from the same-PMID GO:0004674 IDA row; both original source objects are preserved. Proposed replacements: protein serine/threonine kinase activity |
| GO:0106310 protein serine kinase activity | EXP PMID:20625391 Functional polymorphism of the CK2alpha intronless gene play... | MODIFY | Summary: Refine the supported annotation to protein serine/threonine kinase activity. Reason: The full original compares CSNK2A1P with authentic human CSNK2A1, including recombinant/cellular kinase controls and PML-related experiments. The title foregrounds the related locus but the annotated human kinase is actually assayed. The source-specific serine phosphorylation remains positive. The GO enzyme-class convention documented in issue 20114 (https://github.com/geneontology/go-ontology/issues/20114#issuecomment-909198642) places established Ser/Thr kinases such as CK2 in GO:0004674, while retaining GO:0106310 for the exceptional serine-specific class. This is class scoping, not rejection of the residue assay or an is_a generalization. The EXP source remains distinct from the same-PMID GO:0004674 IDA row; both original source objects are preserved. Proposed replacements: protein serine/threonine kinase activity |
| GO:0106310 protein serine kinase activity | EXP PMID:21482717 MDC1 collaborates with TopBP1 in DNA replication checkpoint ... | MODIFY | Summary: Refine the supported annotation to protein serine/threonine kinase activity. Reason: The original RAD9/TOPBP1/MDC1 study uses established CK2-dependent phosphosite recognition to analyze complex assembly. The read Results attribute CK2 phosphorylation to preceding work rather than presenting a new isolated-alpha reaction; the activity remains independently well established and curator-supported. The source-specific serine phosphorylation remains positive. The GO enzyme-class convention documented in issue 20114 (https://github.com/geneontology/go-ontology/issues/20114#issuecomment-909198642) places established Ser/Thr kinases such as CK2 in GO:0004674, while retaining GO:0106310 for the exceptional serine-specific class. This is class scoping, not rejection of the residue assay or an is_a generalization. The EXP source remains distinct from the same-PMID GO:0004674 IDA row; both original source objects are preserved. Proposed replacements: protein serine/threonine kinase activity |
| GO:0106310 protein serine kinase activity | EXP PMID:22017874 Serine 403 phosphorylation of p62/SQSTM1 regulates selective... | MODIFY | Summary: Refine the supported annotation to protein serine/threonine kinase activity. Reason: The complete abstract reports CK2-mediated SQSTM1/p62 Ser403 phosphorylation, enhanced ubiquitin recognition and aggregate clearance. The detailed original Methods remained inaccessible; retain the well-supported kinase class without inventing the construct or assay species. The source-specific serine phosphorylation remains positive. The GO enzyme-class convention documented in issue 20114 (https://github.com/geneontology/go-ontology/issues/20114#issuecomment-909198642) places established Ser/Thr kinases such as CK2 in GO:0004674, while retaining GO:0106310 for the exceptional serine-specific class. This is class scoping, not rejection of the residue assay or an is_a generalization. The EXP source remains distinct from the same-PMID GO:0004674 IDA row; both original source objects are preserved. Proposed replacements: protein serine/threonine kinase activity |
| GO:0106310 protein serine kinase activity | EXP PMID:22184066 Phosphorylation of the transcription factor YY1 by CK2Ξ± prev... | MODIFY | Summary: Refine the supported annotation to protein serine/threonine kinase activity. Reason: The complete abstract reports CK2 phosphorylation of YY1 at Ser118, which protects YY1 from caspase-7 cleavage; the authentic GO-CAM preserves this kinase-to-substrate reaction. Full original assay details were not recovered. The source-specific serine phosphorylation remains positive. The GO enzyme-class convention documented in issue 20114 (https://github.com/geneontology/go-ontology/issues/20114#issuecomment-909198642) places established Ser/Thr kinases such as CK2 in GO:0004674, while retaining GO:0106310 for the exceptional serine-specific class. This is class scoping, not rejection of the residue assay or an is_a generalization. The EXP source remains distinct from the same-PMID GO:0004674 IDA row; both original source objects are preserved. Proposed replacements: protein serine/threonine kinase activity |
| GO:0106310 protein serine kinase activity | EXP PMID:23123191 Functional interaction of protein kinase CK2 and activating ... | MODIFY | Summary: Refine the supported annotation to protein serine/threonine kinase activity. Reason: The complete abstract reports CK2 interaction with ATF4, nuclear bimolecular fluorescence complementation and ATF4 Ser215 phosphorylation. Full original Methods were not recovered, so construct-level details remain curator-grounded. The source-specific serine phosphorylation remains positive. The GO enzyme-class convention documented in issue 20114 (https://github.com/geneontology/go-ontology/issues/20114#issuecomment-909198642) places established Ser/Thr kinases such as CK2 in GO:0004674, while retaining GO:0106310 for the exceptional serine-specific class. This is class scoping, not rejection of the residue assay or an is_a generalization. The EXP source remains distinct from the same-PMID GO:0004674 IDA row; both original source objects are preserved. Proposed replacements: protein serine/threonine kinase activity |
| GO:0106310 protein serine kinase activity | EXP PMID:28512243 Plk1 Phosphorylation of Mre11 Antagonizes the DNA Damage Res... | MODIFY | Summary: Refine the supported annotation to protein serine/threonine kinase activity. Reason: The original measures sequential PLK1/CK2 phosphorylation of MRE11, with CK2 Ser688 and PLK1 Ser649 distinguished in Results, and tests DNA binding and repair consequences. A Discussion numbering discrepancy is not silently corrected in the source. The source-specific serine phosphorylation remains positive. The GO enzyme-class convention documented in issue 20114 (https://github.com/geneontology/go-ontology/issues/20114#issuecomment-909198642) places established Ser/Thr kinases such as CK2 in GO:0004674, while retaining GO:0106310 for the exceptional serine-specific class. This is class scoping, not rejection of the residue assay or an is_a generalization. The EXP source remains distinct from the same-PMID GO:0004674 IDA row; both original source objects are preserved. Proposed replacements: protein serine/threonine kinase activity |
| GO:0106310 protein serine kinase activity | EXP PMID:30699359 Post-translational Regulation of FNIP1 Creates a Rheostat fo... | MODIFY | Summary: Refine the supported annotation to protein serine/threonine kinase activity. Reason: The original uses baculovirus-produced CK2 alpha to phosphorylate the FNIP1-D substrate and maps a multisite serine relay with phosphatase and mutation controls. Human-cell association and recombinant chemistry are distinct positive assays. The source-specific serine phosphorylation remains positive. The GO enzyme-class convention documented in issue 20114 (https://github.com/geneontology/go-ontology/issues/20114#issuecomment-909198642) places established Ser/Thr kinases such as CK2 in GO:0004674, while retaining GO:0106310 for the exceptional serine-specific class. This is class scoping, not rejection of the residue assay or an is_a generalization. The EXP source remains distinct from the same-PMID GO:0004674 IDA row; both original source objects are preserved. Proposed replacements: protein serine/threonine kinase activity |
| GO:0106310 protein serine kinase activity | EXP PMID:30898438 MDC1 Interacts with TOPBP1 to Maintain Chromosomal Stability... | MODIFY | Summary: Refine the supported annotation to protein serine/threonine kinase activity. Reason: The original tests constitutive CK2-dependent MDC1 Ser168/Ser196 phosphorylation and TOPBP1 recruitment during mitosis. The phosphorylation is not described as newly induced by DNA damage. The source-specific serine phosphorylation remains positive. The GO enzyme-class convention documented in issue 20114 (https://github.com/geneontology/go-ontology/issues/20114#issuecomment-909198642) places established Ser/Thr kinases such as CK2 in GO:0004674, while retaining GO:0106310 for the exceptional serine-specific class. This is class scoping, not rejection of the residue assay or an is_a generalization. The EXP source remains distinct from the same-PMID GO:0004674 IDA row; both original source objects are preserved. Proposed replacements: protein serine/threonine kinase activity |
| GO:0106310 protein serine kinase activity | EXP PMID:31439799 Phospho-dependent phase separation of FMRP and CAPRIN1 recap... | MODIFY | Summary: Refine the supported annotation to protein serine/threonine kinase activity. Reason: The recovered author paper assays phosphorylated FMRP/CAPRIN1 low-complexity constructs, phase separation, reticulocyte translation and deadenylation. The kinase treatment is positive, with reconstituted substrate and heterologous translation contexts retained. The source-specific serine phosphorylation remains positive. The GO enzyme-class convention documented in issue 20114 (https://github.com/geneontology/go-ontology/issues/20114#issuecomment-909198642) places established Ser/Thr kinases such as CK2 in GO:0004674, while retaining GO:0106310 for the exceptional serine-specific class. This is class scoping, not rejection of the residue assay or an is_a generalization. The EXP source remains distinct from the same-PMID GO:0004674 IDA row; both original source objects are preserved. Proposed replacements: protein serine/threonine kinase activity |
| GO:0106310 protein serine kinase activity | IEA GO_REF:0000116 | MODIFY | Summary: Refine the supported annotation to protein serine/threonine kinase activity. Reason: RHEA:17989 genuinely describes serine phosphotransfer and the source mapping is not false. The GO enzyme-class convention documented in issue 20114 (https://github.com/geneontology/go-ontology/issues/20114#issuecomment-909198642) places known Ser/Thr enzymes such as CK2 in GO:0004674; the substrate-specific RHEA reaction remains recorded separately. GO:0106310 and GO:0004674 are siblings under protein kinase activity in the current is_a graph, not a parent/child pair. The GO_REF:0000116/RHEA source is distinct from the broader GO_REF:0000120 source and is preserved. The current comment has an inconsistent label/GO:0004712 cross-reference, but the actual curator discussion clarifies the intended enzyme-class scope. Propagation Review Root cause: TERM SCOPING PROBLEM Failure modes: GRANULARITY MISMATCH Sources checked: RHEA:17989 SUPPORTS TRANSFER RHEA:17989 positively records serine phosphotransfer. GO issue 20114 distinguishes substrate-specific reaction records from the known enzyme class; established CK2 Ser/Thr chemistry supports GO:0004674. The RHEA source is preserved and not judged false. Proposed replacements: protein serine/threonine kinase activity |
| GO:1901797 negative regulation of signal transduction by p53 class mediator | IDA PMID:18548200 Transcription factor TAFII250 phosphorylates the acidic doma... | UNDECIDED | Summary: The source-specific negative regulation of signal transduction by p53 class mediator assertion remains unresolved. Reason: Full PMID:18548200 supports CK2 alpha association and Mdm2 phosphorylation, but presents the consequence for p53 turnover as a mechanistic model requiring subsequent molecular/cell-based tests. The biochemical contribution is positive; whether this original directly establishes reduced p53 signal transduction remains unresolved. This is source-specific uncertainty, not a claim that CK2 cannot negatively regulate p53, a missing-full-text rationale, or a wrong-paper verdict. |
| GO:1905337 positive regulation of aggrephagy | IDA PMID:22017874 Serine 403 phosphorylation of p62/SQSTM1 regulates selective... | KEEP AS NON CORE | Summary: Retain the supported positive regulation of aggrephagy context as non-core. Reason: The complete abstract reports SQSTM1/p62 Ser403 phosphorylation by CK2, enhanced ubiquitin affinity and aggregate clearance; the GO-CAM records the kinase-to-receptor step. Retain this performed aggrephagy regulation as non-core, with the unrecovered original Methods explicitly limited. |
| GO:1905818 regulation of chromosome separation | IMP PMID:11704824 Protein kinase CK2 is involved in G2 arrest and apoptosis fo... | KEEP AS NON CORE | Summary: Retain the supported regulation of chromosome separation context as non-core. Reason: The human-cell study reports a p53-dependent mitotic-spindle-checkpoint response after antisense suppression of CK2 alpha/alpha-prime, with inhibitor comparisons. The original main remains abstract-only here; its actual publisher correction supplies omitted figure-caption endings rather than retracting the experiments. Retain the context-dependent chromosome-separation regulation without claiming an alpha-only perturbation. |
| GO:2000042 negative regulation of double-strand break repair via homologous recombination | IDA PMID:28512243 Plk1 Phosphorylation of Mre11 Antagonizes the DNA Damage Res... | KEEP AS NON CORE | Summary: Retain the supported negative regulation of double-strand break repair via homologous recombination context as non-core. Reason: The original assays CK2/PLK1 phosphorylation of MRE11 and reduced DNA-binding/repair activity, preserving the individual phosphosite and priming distinctions. This is a performed negative regulatory step in homologous recombination, compatible with positive effects on other repair substrates and non-core as a specific context. Supporting Evidence: PMID:28512243 subsequent CK2-mediated phosphorylation at Mre11-serine 688 (S688) |
| GO:2001234 negative regulation of apoptotic signaling pathway | IMP PMID:16193064 Caspase-2 primes cancer cells for TRAIL-mediated apoptosis b... | KEEP AS NON CORE | Summary: Retain the supported negative regulation of apoptotic signaling pathway context as non-core. Reason: Recombinant/cellular caspase-2 Ser157 phosphorylation and mutant/TRAIL experiments support suppression of apoptotic signaling. Preserve this mechanistic substrate context as non-core without generalizing an anti-apoptotic outcome to every CK2 substrate. |
| GO:2001234 negative regulation of apoptotic signaling pathway | TAS PMID:28031292 Protein kinase CK2 modulates HSJ1 function through phosphory... | KEEP AS NON CORE | Summary: Retain the supported negative regulation of apoptotic signaling pathway context as non-core. Reason: The original HSJ1/DNAJB2 experiments directly address phosphorylation, ubiquitin-client handling and degradation; their anti-apoptotic interpretation is not a new direct caspase assay. Retain the broad TAS non-core role with this source limit and independent caspase-2 evidence, rather than inventing a specific apoptotic mechanism for the HSJ1 experiment. |
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Download this section (compressed HTML)Q: Does the endogenous 255-aa product retain ATP-dependent kinase activity or CK2-beta assembly after deletion of the N-terminal 136 residues?
Q: Which target-selective cellular experiments distinguish CK2 alpha from alpha-prime in the substrate reactions currently studied with holoenzyme or shared inhibitors?
Q: Does TAFII250-associated CK2 phosphorylation of Mdm2 directly reduce p53 signaling in the original physiological context?
Q: Can the target-specific supplemental evidence behind the PRC1 heterochromatin assertion and the circadian self-binding assertion be resolved?
Q: How should the GO enzyme-class convention in issue 20114 be harmonized with residue-specific UniProt/RHEA assertions, and should the GO:0106310 usage comment point to GO:0004674 rather than its inconsistent GO:0004712 cross-reference? GO:0106311 is obsolete and replaced by GO:0004674, so it is not an active missing threonine-kinase term.
Experiment: Compare sequence-verified full-length and 255-aa products in ATP-dependent substrate phosphorylation and beta-subunit assembly assays, with expression/folding controls.
Experiment: Use paralog-selective depletion and rescue with catalytically active/inactive CSNK2A1 to distinguish direct substrate phosphorylation from inhibitor-sensitive family-wide effects.
Experiment: Test the proposed TAFII250βCK2βMdm2 consequence using phosphosite-resolved Mdm2 constructs and direct p53-turnover/signaling measurements in matched cells.
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