| Substrate name | Binding motif/domain | Type of ubiquitination | Biological function | Key references |
|---|---|---|---|---|
| NRF2/NFE2L2 | DIDLID sequence within the Neh2 domain; distinct from KEAP1-binding DLG/ETGE motifs | Proteolytic; DCAF11/WDR23 promotes NRF2 turnover and suppresses NRF2-dependent transcription | Negative regulation of oxidative stress and xenobiotic-response programs; controls cytoprotective gene expression independently of KEAP1-CUL3 | (pqac-00000004, pqac-00000006, pqac-00000009) |
| p21Cip1 (CDKN1A) | Direct interaction reported; specific DCAF11-binding degron not defined in the cited study | Proteolytic; CRL4B^DCAF11 ubiquitinates p21 at K16, K154, K161, and K163 | Promotes cell-cycle progression, especially S-phase progression, in osteosarcoma cells by reducing the CDK inhibitor p21 | (pqac-00000002, pqac-00000007) |
| SLBP (Stem-loop binding protein) | Phosphorylation-dependent recognition of the N-terminal region containing Thr60/Thr61; Thr61 phosphorylation is critical | Proteolytic; CRL4-DCAF11 mediates end-of-S-phase degradation | Terminates histone mRNA metabolism/histone synthesis at the S/G2 transition and supports cell viability | (pqac-00000003, pqac-00000008) |
| GEN-1 (GEN1 Holliday junction resolvase) | Evolutionarily conserved WDR23 substrate; precise human binding motif not specified in the cited context | Likely proteolytic or inhibitory turnover-associated ubiquitination in the nucleus; isoform-dependent regulation described | DNA damage repair/Holliday junction resolution and regulation of double-strand break repair capacity | (pqac-00000014) |
| IDE (Insulin-degrading enzyme) | No evidence in the cited study for direct physical binding to DCAF11/WDR23; regulation is indirect via NRF2, a direct WDR23 target | Indirect effect rather than demonstrated direct substrate ubiquitination; WDR23 loss increases IDE expression through NRF2-dependent transcription | Insulin homeostasis and hepatic insulin clearance; altered IDE levels change circulating insulin and insulin signaling | (pqac-00000005) |
| Influenza A PB2 protein | Unconventional bimodal recruitment involving both DDB1 adaptor and DCAF substrate receptors including DCAF11; precise PB2 degron not defined | Non-proteolytic K29-linked ubiquitination | Proviral regulation of influenza replication; supports optimal viral cycle progression and virion production | (pqac-00000011) |


*Table: This table summarizes the main proteins reported to be regulated by the human DCAF11/WDR23-containing CRL4 ubiquitin ligase system, including recognition features, ubiquitination outcome, and biological role. It is useful for distinguishing direct proteolytic substrates from indirect or non-proteolytic regulatory effects.*