DNAAF3

Summary: Cytoplasmic assembly factor with enrichment in DynAPs.

Reason: The official SL-0086 assertion maps exactly to cytoplasm. DNAAF3 acts before axonemal deployment of dynein motors, and the Xenopus DNAAF3 fusion in PMID:30561330 Figure 2C occupies cytoplasmic foci with a surrounding cytoplasmic pool. Retain the general cytoplasmic location alongside the specifically supported DynAP enrichment; neither assertion makes DNAAF3 a mature axonemal motor.

Summary: Inherited participation in motile-cilium assembly through maturation of its dynein-arm components.

Reason: The human leaf PTN002502984 descends from PTN001858482 in PTHR22118, and the literal IBD grounding includes genuine human experimental evidence. DNAAF3 promotes maturation of the outer and inner dynein arms that form part of a functional motile cilium. Human patient cilia lack these arms, while ortholog rescue and biochemical experiments identify a cytoplasmic assembly defect: abnormal heavy/intermediate-chain complexes form but fail to become assembly competent. Persistent ciliary projections therefore do not negate participation in their constituent assembly. This is a core role. GO:0036158 and GO:0036159 are not current is_a/part_of descendants of GO:0044458, so those separately represented arm-assembly processes do not replace this inherited claim.

Summary: DNAAF3 contributes to assembly of functional motile cilia by maturing their axonemal dynein arms.

Reason: PMID:22387996 directly shows loss of both dynein-arm classes in human patient respiratory cilia; zebrafish knockdown leaves ciliary morphology and number largely intact while disrupting arms and motility. In Chlamydomonas, rescue and cytoplasmic heavy/intermediate-chain assays show that PF22/DNAAF3 supports maturation into assembly-competent motors. The performed work is dynein-component assembly, not merely an effect on beating or a claim that DNAAF3 builds the ciliary membrane. GO:0044458 encompasses arrangement of components into a motile cilium, making this core participation despite persistence of projections. The specific arm terms are retained separately because neither is an is_a/part_of descendant of GO:0044458.

Summary: Inherited axonemal dynein assembly includes both outer and inner arms.

Reason: PTHR22118 places the human target beneath the stated PTN001858482 ancestral node, whose GO:0070286 IBD is experimentally grounded. Refine the broader assembly term to its verified outer-arm and inner-arm assembly children using the target-specific evidence in PMID:22387996. This preserves the positive phylogenetic judgment and does not infer catalytic motor activity.

Proposed replacements: outer dynein arm assembly inner dynein arm assembly

Summary: Correct family mapping can be refined to outer and inner dynein-arm assembly.

Reason: IPR039304 is the DNAAF3 family, with an actual human target match over canonical residues 1–470 and an explicit GO:0070286 mapping. The InterPro entry cites PMID:22387996 and describes preassembly of both inner and outer arms. Refine the correct broad mapping to those two specific assembly processes without assigning an independent chaperone or motor activity.

Proposed replacements: outer dynein arm assembly inner dynein arm assembly

Summary: Experimental assembly phenotype resolved to outer and inner dynein arms.

Reason: The human and ortholog findings in PMID:22387996 support DNAAF3-dependent dynein-arm preassembly. Outer dynein arm assembly and inner dynein arm assembly are verified is_a children of the existing term and express the two supported target-level components. Preserve the original experimental assertion while making its biological scope more specific.

Proposed replacements: outer dynein arm assembly inner dynein arm assembly

Summary: DynAP localization from a correctly mapped, experimentally grounded ortholog inference.

Reason: SL-0542 maps exactly to dynein axonemal particle. The human UniProt location traces to Xenopus laevis DNAAF3 Q32NQ7, whose direct IDA evidence is PMID:30561330. The original Figure 2C shows tagged Xenopus DNAAF3 co-localizing with Ktu in cytoplasmic DynAPs. Retain the conserved location, while distinguishing it from direct imaging of endogenous human DNAAF3 or demonstrated localization of every predicted human isoform.

Summary: Experimentally localized Xenopus ortholog supports the human DynAP transfer.

Reason: The literal donor Q32NQ7 is Xenopus laevis dnaaf3 and carries direct IDA to GO:0120293 from PMID:30561330. Methods identify DNAAF3 cloned from a Xenopus cDNA library, and Figure 2C shows the DNAAF3 fluorescent fusion in Ktu-positive DynAPs. This positive source evidence supports the curator's ortholog transfer. Human staining in the same paper assays LRRC6 and DNAI1, so it is not presented as direct human DNAAF3 imaging.