ID   DJC16_HUMAN             Reviewed;         782 AA.
AC   Q9Y2G8; Q68D57; Q86X32; Q8N5P4;
DT   30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2006, sequence version 3.
DT   28-JAN-2026, entry version 168.
DE   RecName: Full=DnaJ homolog subfamily C member 16;
DE   AltName: Full=Endoplasmic reticulum DNA J domain-containing protein 8 {ECO:0000303|PubMed:32492081};
DE            Short=ER-resident protein ERdj8;
DE            Short=ERdj8;
DE   Flags: Precursor;
GN   Name=DNAJC16; Synonyms=ERDJ8, KIAA0962;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Brain;
RX   PubMed=10231032; DOI=10.1093/dnares/6.1.63;
RA   Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N.,
RA   Tanaka A., Kotani H., Nomura N., Ohara O.;
RT   "Prediction of the coding sequences of unidentified human genes. XIII. The
RT   complete sequences of 100 new cDNA clones from brain which code for large
RT   proteins in vitro.";
RL   DNA Res. 6:63-70(1999).
RN   [2]
RP   SEQUENCE REVISION.
RX   PubMed=12168954; DOI=10.1093/dnares/9.3.99;
RA   Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.;
RT   "Construction of expression-ready cDNA clones for KIAA genes: manual
RT   curation of 330 KIAA cDNA clones.";
RL   DNA Res. 9:99-106(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   TISSUE=Retina;
RX   PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA   Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA   Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA   Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA   Wiemann S., Schupp I.;
RT   "The full-ORF clone resource of the German cDNA consortium.";
RL   BMC Genomics 8:399-399(2007).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16710414; DOI=10.1038/nature04727;
RA   Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA   Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA   Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA   Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA   Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA   Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA   Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA   Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA   Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA   Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA   Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA   Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA   Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA   Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA   Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA   Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA   Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA   Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA   Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA   McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA   Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA   Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA   Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA   Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA   Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA   White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA   Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA   Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA   Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT   "The DNA sequence and biological annotation of human chromosome 1.";
RL   Nature 441:315-321(2006).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Skin, and Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF HIS-57; CYS-171 AND
RP   CYS-174.
RX   PubMed=32492081; DOI=10.1083/jcb.201903127;
RA   Yamamoto Y.H., Kasai A., Omori H., Takino T., Sugihara M., Umemoto T.,
RA   Hamasaki M., Hatta T., Natsume T., Morimoto R.I., Arai R., Waguri S.,
RA   Sato M., Sato K., Bar-Nun S., Yoshimori T., Noda T., Nagata K.;
RT   "ERdj8 governs the size of autophagosomes during the formation process.";
RL   J. Cell Biol. 219:0-0(2020).
CC   -!- FUNCTION: Plays an important role in regulating the size of
CC       autophagosomes during the formation process.
CC       {ECO:0000269|PubMed:32492081}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000269|PubMed:32492081}; Single-pass type IV membrane protein
CC       {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q9Y2G8-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9Y2G8-2; Sequence=VSP_018583;
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA76806.2; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AB023179; BAA76806.2; ALT_INIT; mRNA.
DR   EMBL; CR749259; CAH18115.1; -; mRNA.
DR   EMBL; CR749567; CAH18363.1; -; mRNA.
DR   EMBL; AL512883; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AL121992; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC031991; AAH31991.1; -; mRNA.
DR   EMBL; BC047363; AAH47363.1; -; mRNA.
DR   CCDS; CCDS30606.1; -. [Q9Y2G8-1]
DR   CCDS; CCDS72710.1; -. [Q9Y2G8-2]
DR   RefSeq; NP_001274740.1; NM_001287811.2. [Q9Y2G8-2]
DR   RefSeq; NP_056106.1; NM_015291.4. [Q9Y2G8-1]
DR   AlphaFoldDB; Q9Y2G8; -.
DR   SMR; Q9Y2G8; -.
DR   BioGRID; 116926; 288.
DR   FunCoup; Q9Y2G8; 1374.
DR   MINT; Q9Y2G8; -.
DR   STRING; 9606.ENSP00000365007; -.
DR   GlyCosmos; Q9Y2G8; 1 site, No reported glycans.
DR   GlyGen; Q9Y2G8; 1 site.
DR   iPTMnet; Q9Y2G8; -.
DR   PhosphoSitePlus; Q9Y2G8; -.
DR   SwissPalm; Q9Y2G8; -.
DR   BioMuta; DNAJC16; -.
DR   DMDM; 108936027; -.
DR   jPOST; Q9Y2G8; -.
DR   MassIVE; Q9Y2G8; -.
DR   PaxDb; 9606-ENSP00000365007; -.
DR   PeptideAtlas; Q9Y2G8; -.
DR   ProteomicsDB; 85773; -. [Q9Y2G8-1]
DR   ProteomicsDB; 85774; -. [Q9Y2G8-2]
DR   Pumba; Q9Y2G8; -.
DR   Antibodypedia; 46653; 28 antibodies from 14 providers.
DR   DNASU; 23341; -.
DR   Ensembl; ENST00000375847.8; ENSP00000365007.3; ENSG00000116138.14. [Q9Y2G8-1]
DR   Ensembl; ENST00000616884.4; ENSP00000480224.1; ENSG00000116138.14. [Q9Y2G8-2]
DR   GeneID; 23341; -.
DR   KEGG; hsa:23341; -.
DR   MANE-Select; ENST00000375847.8; ENSP00000365007.3; NM_015291.4; NP_056106.1.
DR   UCSC; uc001aws.4; human. [Q9Y2G8-1]
DR   AGR; HGNC:29157; -.
DR   ClinPGx; PA142671964; -.
DR   CTD; 23341; -.
DR   DisGeNET; 23341; -.
DR   GeneCards; DNAJC16; -.
DR   HGNC; HGNC:29157; DNAJC16.
DR   HPA; ENSG00000116138; Low tissue specificity.
DR   MIM; 619973; gene.
DR   OpenTargets; ENSG00000116138; -.
DR   VEuPathDB; HostDB:ENSG00000116138; -.
DR   eggNOG; KOG0715; Eukaryota.
DR   GeneTree; ENSGT00940000155851; -.
DR   HOGENOM; CLU_020140_0_1_1; -.
DR   InParanoid; Q9Y2G8; -.
DR   OMA; QPEFAST; -.
DR   OrthoDB; 10065037at2759; -.
DR   PAN-GO; Q9Y2G8; 0 GO annotations based on evolutionary models.
DR   PhylomeDB; Q9Y2G8; -.
DR   PathwayCommons; Q9Y2G8; -.
DR   SignaLink; Q9Y2G8; -.
DR   Agora; ENSG00000116138; -.
DR   BioGRID-ORCS; 23341; 19 hits in 1157 CRISPR screens.
DR   ChiTaRS; DNAJC16; human.
DR   GenomeRNAi; 23341; -.
DR   Pharos; Q9Y2G8; Tdark.
DR   PRO; PR:Q9Y2G8; -.
DR   Proteomes; UP000005640; Chromosome 1.
DR   RNAct; Q9Y2G8; protein.
DR   Bgee; ENSG00000116138; Expressed in right lobe of liver and 205 other cell types or tissues.
DR   ExpressionAtlas; Q9Y2G8; baseline and differential.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IDA:UniProtKB.
DR   GO; GO:0016243; P:regulation of autophagosome size; IMP:UniProtKB.
DR   CDD; cd06257; DnaJ; 1.
DR   CDD; cd02963; TRX_DnaJ; 1.
DR   Gene3D; 1.10.287.110; DnaJ domain; 1.
DR   Gene3D; 3.40.30.10; Glutaredoxin; 1.
DR   InterPro; IPR052448; DnaJ_C16_autophagy_reg.
DR   InterPro; IPR001623; DnaJ_domain.
DR   InterPro; IPR018253; DnaJ_domain_CS.
DR   InterPro; IPR043361; DNAJC16_TRX.
DR   InterPro; IPR036869; J_dom_sf.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   InterPro; IPR013766; Thioredoxin_domain.
DR   PANTHER; PTHR44303; DNAJ HOMOLOG SUBFAMILY C MEMBER 16; 1.
DR   PANTHER; PTHR44303:SF2; DNAJ HOMOLOG SUBFAMILY C MEMBER 16; 1.
DR   Pfam; PF00226; DnaJ; 1.
DR   Pfam; PF00085; Thioredoxin; 1.
DR   PRINTS; PR00625; JDOMAIN.
DR   SMART; SM00271; DnaJ; 1.
DR   SUPFAM; SSF46565; Chaperone J-domain; 1.
DR   SUPFAM; SSF52833; Thioredoxin-like; 1.
DR   PROSITE; PS00636; DNAJ_1; 1.
DR   PROSITE; PS50076; DNAJ_2; 1.
DR   PROSITE; PS51352; THIOREDOXIN_2; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Autophagy; Endoplasmic reticulum; Glycoprotein;
KW   Membrane; Proteomics identification; Reference proteome; Signal;
KW   Transmembrane; Transmembrane helix.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   CHAIN           26..782
FT                   /note="DnaJ homolog subfamily C member 16"
FT                   /id="PRO_0000236683"
FT   TOPO_DOM        26..535
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        536..556
FT                   /note="Helical; Anchor for type IV membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        557..782
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          29..93
FT                   /note="J"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00286"
FT   DOMAIN          119..247
FT                   /note="Thioredoxin"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00691"
FT   REGION          562..593
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        563..582
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        583..593
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        631
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         1..312
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:17974005"
FT                   /id="VSP_018583"
FT   MUTAGEN         57
FT                   /note="H->Q: Does not lead to enlargement of autophagosomes
FT                   when overexpressed."
FT                   /evidence="ECO:0000269|PubMed:32492081"
FT   MUTAGEN         171
FT                   /note="C->A: Does not lead to enlargement of autophagosomes
FT                   when overexpressed; when associated with A-174."
FT                   /evidence="ECO:0000269|PubMed:32492081"
FT   MUTAGEN         174
FT                   /note="C->A: Does not lead to enlargement of autophagosomes
FT                   when overexpressed; when associated with A-171."
FT                   /evidence="ECO:0000269|PubMed:32492081"
SQ   SEQUENCE   782 AA;  90591 MW;  42D564BBC6EED981 CRC64;
     MEVRKLSISW QFLIVLVLIL QILSALDFDP YRVLGVSRTA SQADIKKAYK KLAREWHPDK
     NKDPGAEDKF IQISKAYEIL SNEEKRSNYD QYGDAGENQG YQKQQQQREY RFRHFHENFY
     FDESFFHFPF NSERRDSIDE KYLLHFSHYV NEVVPDSFKK PYLIKITSDW CFSCIHIEPV
     WKEVIQELEE LGVGIGVVHA GYERRLAHHL GAHSTPSILG IINGKISFFH NAVVRENLRQ
     FVESLLPGNL VEKVTNKNYV RFLSGWQQEN KPHVLLFDQT PIVPLLYKLT AFAYKDYLSF
     GYVYVGLRGT EEMTRRYNIN IYAPTLLVFK EHINRPADVI QARGMKKQII DDFITRNKYL
     LAARLTSQKL FHELCPVKRS HRQRKYCVVL LTAETTKLSK PFEAFLSFAL ANTQDTVRFV
     HVYSNRQQEF ADTLLPDSEA FQGKSAVSIL ERRNTAGRVV YKTLEDPWIG SESDKFILLG
     YLDQLRKDPA LLSSEAVLPD LTDELAPVFL LRWFYSASDY ISDCWDSIFH NNWREMMPLL
     SLIFSALFIL FGTVIVQAFS DSNDERESSP PEKEEAQEKT GKTEPSFTKE NSSKIPKKGF
     VEVTELTDVT YTSNLVRLRP GHMNVVLILS NSTKTSLLQK FALEVYTFTG SSCLHFSFLS
     LDKHREWLEY LLEFAQDAAP IPNQYDKHFM ERDYTGYVLA LNGHKKYFCL FKPQKTVEEE
     EAIGSCSDVD SSLYLGESRG KPSCGLGSRP IKGKLSKLSL WMERLLEGSL QRFYIPSWPE
     LD
//