DPYSL2

# yaml-language-server: $schema=../../../src/ai_gene_review/schema/gene_review.yaml
id: Q16555
gene_symbol: DPYSL2
product_type: PROTEIN
status: DRAFT
taxon:
  id: NCBITaxon:9606
  label: Homo sapiens
description: 'Dihydropyrimidinase-related protein 2 (DPYSL2/CRMP2), a brain-enriched cytoplasmic protein
  of the collapsin-response-mediator (CRMP) / dihydropyrimidinase family. Although it belongs to the metallo-dependent
  hydrolase superfamily, it lacks the conserved metal-cofactor-binding residues and has no dihydropyrimidinase
  activity - it is a catalytically dead family member. CRMP2 is a key cytoskeletal regulator in semaphorin-3A
  signaling: it binds tubulin heterodimers to promote microtubule assembly and axon specification/growth,
  mediates growth-cone collapse and neuronal polarity, and is inactivated by GSK3B/CDK5/ROCK phosphorylation.
  It forms homo- and hetero-tetramers with other CRMPs and also participates in vesicle/endocytic trafficking.'
alternative_products:
- name: '1'
  id: Q16555-1
- name: '2'
  id: Q16555-2
  sequence_note: VSP_044941
existing_annotations:
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: is_active_in
  review:
    summary: 'cytosol: a core subcellular location for DPYSL2 (cytoplasmic/cytoskeletal CRMP).'
    action: ACCEPT
    reason: Correct core localization for a cytoskeleton-associated cytoplasmic protein.
- term:
    id: GO:0006208
    label: pyrimidine nucleobase catabolic process
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: involved_in
  negated: true
  review:
    summary: 'NOT: DPYSL2 is not involved in pyrimidine nucleobase catabolism (the process counterpart
      of the absent dihydropyrimidinase activity).'
    action: ACCEPT
    reason: Correct negation, consistent with loss of catalytic activity. Retain.
    supported_by:
    - reference_id: file:human/DPYSL2/DPYSL2-uniprot.txt
      supporting_text: Lacks most of the conserved residues that are essential for
- term:
    id: GO:0016812
    label: hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: enables
  review:
    summary: Positive 'hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic
      amides' propagated from the metallo-hydrolase fold signature (IBA). DPYSL2 lacks the catalytic metal
      site, and the curated dihydropyrimidinase activity is itself NOT-ed.
    action: REMOVE
    reason: 'Domain/phylogenetic over-propagation refutable on biological grounds: the metal-cofactor-binding
      residues are absent (UniProt CAUTION), so no metallo-hydrolase activity is supported; the real function
      is a non-catalytic cytoskeletal regulator. Same basis as the DPYSL5 review.'
    supported_by:
    - reference_id: file:human/DPYSL2/DPYSL2-uniprot.txt
      supporting_text: Lacks most of the conserved residues that are essential for
    - reference_id: file:human/DPYSL2/DPYSL2-uniprot.txt
      supporting_text: Belongs to the metallo-dependent hydrolases superfamily.
- term:
    id: GO:0004157
    label: dihydropyrimidinase activity
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: enables
  negated: true
  review:
    summary: 'NOT: DPYSL2 does not have dihydropyrimidinase activity. It belongs to the metallo-dependent
      hydrolase superfamily but lacks the conserved metal-cofactor-binding residues required for catalysis
      (UniProt CAUTION).'
    action: ACCEPT
    reason: 'Correct, important negation: a catalytically dead family member. Retain.'
    supported_by:
    - reference_id: file:human/DPYSL2/DPYSL2-uniprot.txt
      supporting_text: Lacks most of the conserved residues that are essential for
- term:
    id: GO:0005737
    label: cytoplasm
  evidence_type: IEA
  original_reference_id: GO_REF:0000002
  qualifier: located_in
  review:
    summary: 'cytoplasm: a core subcellular location for DPYSL2 (cytoplasmic/cytoskeletal CRMP).'
    action: ACCEPT
    reason: Correct core localization for a cytoskeleton-associated cytoplasmic protein.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  qualifier: located_in
  review:
    summary: 'cytosol: a core subcellular location for DPYSL2 (cytoplasmic/cytoskeletal CRMP).'
    action: ACCEPT
    reason: Correct core localization for a cytoskeleton-associated cytoplasmic protein.
- term:
    id: GO:0005856
    label: cytoskeleton
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  qualifier: located_in
  review:
    summary: 'cytoskeleton: a core subcellular location for DPYSL2 (cytoplasmic/cytoskeletal CRMP).'
    action: ACCEPT
    reason: Correct core localization for a cytoskeleton-associated cytoplasmic protein.
- term:
    id: GO:0016020
    label: membrane
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  qualifier: located_in
  review:
    summary: 'membrane: a secondary/broad or context-specific localization for DPYSL2.'
    action: KEEP_AS_NON_CORE
    reason: Plausible but non-core (broad term, division-/synapse-specific, or high-throughput proteomics).
- term:
    id: GO:0016787
    label: hydrolase activity
  evidence_type: IEA
  original_reference_id: GO_REF:0000002
  qualifier: enables
  review:
    summary: Positive 'hydrolase activity' propagated from the metallo-hydrolase fold signature (IEA).
      DPYSL2 lacks the catalytic metal site, and the curated dihydropyrimidinase activity is itself NOT-ed.
    action: REMOVE
    reason: 'Domain/phylogenetic over-propagation refutable on biological grounds: the metal-cofactor-binding
      residues are absent (UniProt CAUTION), so no metallo-hydrolase activity is supported; the real function
      is a non-catalytic cytoskeletal regulator. Same basis as the DPYSL5 review.'
    supported_by:
    - reference_id: file:human/DPYSL2/DPYSL2-uniprot.txt
      supporting_text: Lacks most of the conserved residues that are essential for
    - reference_id: file:human/DPYSL2/DPYSL2-uniprot.txt
      supporting_text: Belongs to the metallo-dependent hydrolases superfamily.
- term:
    id: GO:0016810
    label: hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
  evidence_type: IEA
  original_reference_id: GO_REF:0000002
  qualifier: enables
  review:
    summary: Positive 'hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds' propagated
      from the metallo-hydrolase fold signature (IEA). DPYSL2 lacks the catalytic metal site, and the
      curated dihydropyrimidinase activity is itself NOT-ed.
    action: REMOVE
    reason: 'Domain/phylogenetic over-propagation refutable on biological grounds: the metal-cofactor-binding
      residues are absent (UniProt CAUTION), so no metallo-hydrolase activity is supported; the real function
      is a non-catalytic cytoskeletal regulator. Same basis as the DPYSL5 review.'
    supported_by:
    - reference_id: file:human/DPYSL2/DPYSL2-uniprot.txt
      supporting_text: Lacks most of the conserved residues that are essential for
    - reference_id: file:human/DPYSL2/DPYSL2-uniprot.txt
      supporting_text: Belongs to the metallo-dependent hydrolases superfamily.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:21900206
  qualifier: enables
  review:
    summary: Specific protein interaction (PMID:21900206); supports DPYSL2's scaffold/adapter role but
      the generic 'protein binding' term is uninformative.
    action: KEEP_AS_NON_CORE
    reason: Real interaction kept as non-core supporting evidence; the informative function is captured
      in core_functions.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:24722188
  qualifier: enables
  review:
    summary: Specific protein interaction (PMID:24722188); supports DPYSL2's scaffold/adapter role but
      the generic 'protein binding' term is uninformative.
    action: KEEP_AS_NON_CORE
    reason: Real interaction kept as non-core supporting evidence; the informative function is captured
      in core_functions.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:25416956
  qualifier: enables
  review:
    summary: Generic 'protein binding' from a high-throughput interactome screen (PMID:25416956).
    action: MARK_AS_OVER_ANNOTATED
    reason: High-throughput protein binding is uninformative about molecular function (curation guideline).
      Over-annotation.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:28514442
  qualifier: enables
  review:
    summary: Generic 'protein binding' from a high-throughput interactome screen (PMID:28514442).
    action: MARK_AS_OVER_ANNOTATED
    reason: High-throughput protein binding is uninformative about molecular function (curation guideline).
      Over-annotation.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:29892012
  qualifier: enables
  review:
    summary: Generic 'protein binding' from a high-throughput interactome screen (PMID:29892012).
    action: MARK_AS_OVER_ANNOTATED
    reason: High-throughput protein binding is uninformative about molecular function (curation guideline).
      Over-annotation.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:31515488
  qualifier: enables
  review:
    summary: Generic 'protein binding' from a high-throughput interactome screen (PMID:31515488).
    action: MARK_AS_OVER_ANNOTATED
    reason: High-throughput protein binding is uninformative about molecular function (curation guideline).
      Over-annotation.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:32296183
  qualifier: enables
  review:
    summary: Generic 'protein binding' from a high-throughput interactome screen (PMID:32296183).
    action: MARK_AS_OVER_ANNOTATED
    reason: High-throughput protein binding is uninformative about molecular function (curation guideline).
      Over-annotation.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:32814053
  qualifier: enables
  review:
    summary: Generic 'protein binding' from a high-throughput interactome screen (PMID:32814053).
    action: MARK_AS_OVER_ANNOTATED
    reason: High-throughput protein binding is uninformative about molecular function (curation guideline).
      Over-annotation.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:33961781
  qualifier: enables
  review:
    summary: Generic 'protein binding' from a high-throughput interactome screen (PMID:33961781).
    action: MARK_AS_OVER_ANNOTATED
    reason: High-throughput protein binding is uninformative about molecular function (curation guideline).
      Over-annotation.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:36950384
  qualifier: enables
  review:
    summary: Specific protein interaction (PMID:36950384); supports DPYSL2's scaffold/adapter role but
      the generic 'protein binding' term is uninformative.
    action: KEEP_AS_NON_CORE
    reason: Real interaction kept as non-core supporting evidence; the informative function is captured
      in core_functions.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:40205054
  qualifier: enables
  review:
    summary: Specific protein interaction (PMID:40205054); supports DPYSL2's scaffold/adapter role but
      the generic 'protein binding' term is uninformative.
    action: KEEP_AS_NON_CORE
    reason: Real interaction kept as non-core supporting evidence; the informative function is captured
      in core_functions.
- term:
    id: GO:0042802
    label: identical protein binding
  evidence_type: IPI
  original_reference_id: PMID:21516116
  qualifier: enables
  review:
    summary: 'Identical protein binding: DPYSL2 forms homo- and hetero-tetramers with other CRMP-family
      members.'
    action: KEEP_AS_NON_CORE
    reason: Real oligomerization but a generic term; non-core.
    supported_by:
    - reference_id: file:human/DPYSL2/DPYSL2-uniprot.txt
      supporting_text: Homotetramer
- term:
    id: GO:0042802
    label: identical protein binding
  evidence_type: IPI
  original_reference_id: PMID:25416956
  qualifier: enables
  review:
    summary: 'Identical protein binding: DPYSL2 forms homo- and hetero-tetramers with other CRMP-family
      members.'
    action: KEEP_AS_NON_CORE
    reason: Real oligomerization but a generic term; non-core.
    supported_by:
    - reference_id: file:human/DPYSL2/DPYSL2-uniprot.txt
      supporting_text: Homotetramer
- term:
    id: GO:0042802
    label: identical protein binding
  evidence_type: IPI
  original_reference_id: PMID:29892012
  qualifier: enables
  review:
    summary: 'Identical protein binding: DPYSL2 forms homo- and hetero-tetramers with other CRMP-family
      members.'
    action: KEEP_AS_NON_CORE
    reason: Real oligomerization but a generic term; non-core.
    supported_by:
    - reference_id: file:human/DPYSL2/DPYSL2-uniprot.txt
      supporting_text: Homotetramer
- term:
    id: GO:0042802
    label: identical protein binding
  evidence_type: IPI
  original_reference_id: PMID:31515488
  qualifier: enables
  review:
    summary: 'Identical protein binding: DPYSL2 forms homo- and hetero-tetramers with other CRMP-family
      members.'
    action: KEEP_AS_NON_CORE
    reason: Real oligomerization but a generic term; non-core.
    supported_by:
    - reference_id: file:human/DPYSL2/DPYSL2-uniprot.txt
      supporting_text: Homotetramer
- term:
    id: GO:0042802
    label: identical protein binding
  evidence_type: IPI
  original_reference_id: PMID:32296183
  qualifier: enables
  review:
    summary: 'Identical protein binding: DPYSL2 forms homo- and hetero-tetramers with other CRMP-family
      members.'
    action: KEEP_AS_NON_CORE
    reason: Real oligomerization but a generic term; non-core.
    supported_by:
    - reference_id: file:human/DPYSL2/DPYSL2-uniprot.txt
      supporting_text: Homotetramer
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: IDA
  original_reference_id: GO_REF:0000052
  qualifier: located_in
  review:
    summary: 'cytosol: a core subcellular location for DPYSL2 (cytoplasmic/cytoskeletal CRMP).'
    action: ACCEPT
    reason: Correct core localization for a cytoskeleton-associated cytoplasmic protein.
- term:
    id: GO:0015630
    label: microtubule cytoskeleton
  evidence_type: IDA
  original_reference_id: GO_REF:0000052
  qualifier: located_in
  review:
    summary: 'microtubule cytoskeleton: a core subcellular location for DPYSL2 (cytoplasmic/cytoskeletal
      CRMP).'
    action: ACCEPT
    reason: Correct core localization for a cytoskeleton-associated cytoplasmic protein.
- term:
    id: GO:0045171
    label: intercellular bridge
  evidence_type: IDA
  original_reference_id: GO_REF:0000052
  qualifier: located_in
  review:
    summary: 'intercellular bridge: a secondary/broad or context-specific localization for DPYSL2.'
    action: KEEP_AS_NON_CORE
    reason: Plausible but non-core (broad term, division-/synapse-specific, or high-throughput proteomics).
- term:
    id: GO:0072686
    label: mitotic spindle
  evidence_type: IDA
  original_reference_id: GO_REF:0000052
  qualifier: located_in
  review:
    summary: 'mitotic spindle: a secondary/broad or context-specific localization for DPYSL2.'
    action: KEEP_AS_NON_CORE
    reason: Plausible but non-core (broad term, division-/synapse-specific, or high-throughput proteomics).
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: IDA
  original_reference_id: PMID:20801876
  qualifier: located_in
  review:
    summary: 'cytosol: a core subcellular location for DPYSL2 (cytoplasmic/cytoskeletal CRMP).'
    action: ACCEPT
    reason: Correct core localization for a cytoskeleton-associated cytoplasmic protein.
- term:
    id: GO:0005874
    label: microtubule
  evidence_type: IDA
  original_reference_id: PMID:20801876
  qualifier: colocalizes_with
  review:
    summary: 'microtubule: a core subcellular location for DPYSL2 (cytoplasmic/cytoskeletal CRMP).'
    action: ACCEPT
    reason: Correct core localization for a cytoskeleton-associated cytoplasmic protein.
- term:
    id: GO:0006897
    label: endocytosis
  evidence_type: IMP
  original_reference_id: PMID:20801876
  qualifier: involved_in
  review:
    summary: 'endocytosis: a broader/secondary process for DPYSL2.'
    action: KEEP_AS_NON_CORE
    reason: Valid but non-core relative to the cytoskeletal-regulation function.
- term:
    id: GO:0016020
    label: membrane
  evidence_type: IDA
  original_reference_id: PMID:20801876
  qualifier: colocalizes_with
  review:
    summary: 'membrane: a secondary/broad or context-specific localization for DPYSL2.'
    action: KEEP_AS_NON_CORE
    reason: Plausible but non-core (broad term, division-/synapse-specific, or high-throughput proteomics).
- term:
    id: GO:0070062
    label: extracellular exosome
  evidence_type: HDA
  original_reference_id: PMID:20458337
  qualifier: located_in
  review:
    summary: 'extracellular exosome: a secondary/broad or context-specific localization for DPYSL2.'
    action: KEEP_AS_NON_CORE
    reason: Plausible but non-core (broad term, division-/synapse-specific, or high-throughput proteomics).
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-399951
  qualifier: located_in
  review:
    summary: 'cytosol: a core subcellular location for DPYSL2 (cytoplasmic/cytoskeletal CRMP).'
    action: ACCEPT
    reason: Correct core localization for a cytoskeleton-associated cytoplasmic protein.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-399944
  qualifier: located_in
  review:
    summary: 'cytosol: a core subcellular location for DPYSL2 (cytoplasmic/cytoskeletal CRMP).'
    action: ACCEPT
    reason: Correct core localization for a cytoskeleton-associated cytoplasmic protein.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-399947
  qualifier: located_in
  review:
    summary: 'cytosol: a core subcellular location for DPYSL2 (cytoplasmic/cytoskeletal CRMP).'
    action: ACCEPT
    reason: Correct core localization for a cytoskeleton-associated cytoplasmic protein.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-443783
  qualifier: located_in
  review:
    summary: 'cytosol: a core subcellular location for DPYSL2 (cytoplasmic/cytoskeletal CRMP).'
    action: ACCEPT
    reason: Correct core localization for a cytoskeleton-associated cytoplasmic protein.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:19235893
  qualifier: enables
  review:
    summary: Specific protein interaction (PMID:19235893); supports DPYSL2's scaffold/adapter role but
      the generic 'protein binding' term is uninformative.
    action: KEEP_AS_NON_CORE
    reason: Real interaction kept as non-core supporting evidence; the informative function is captured
      in core_functions.
- term:
    id: GO:0007010
    label: cytoskeleton organization
  evidence_type: ISS
  original_reference_id: GO_REF:0000024
  qualifier: involved_in
  review:
    summary: 'cytoskeleton organization: a core neuronal/cytoskeletal process for the CRMP family (DPYSL2
      acts in semaphorin-driven cytoskeleton remodeling and neurite/axon development).'
    action: ACCEPT
    reason: Core biological process for a CRMP-family cytoskeletal regulator.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:16260607
  qualifier: enables
  review:
    summary: Specific protein interaction (PMID:16260607); supports DPYSL2's scaffold/adapter role but
      the generic 'protein binding' term is uninformative.
    action: KEEP_AS_NON_CORE
    reason: Real interaction kept as non-core supporting evidence; the informative function is captured
      in core_functions.
- term:
    id: GO:0004157
    label: dihydropyrimidinase activity
  evidence_type: TAS
  original_reference_id: PMID:8973361
  qualifier: enables
  review:
    summary: Legacy positive dihydropyrimidinase activity (TAS, PMID:8973361) from an early report, superseded
      by the finding that DPYSL2 lacks the catalytic metal-binding residues.
    action: REMOVE
    reason: Outdated enzymatic claim, contradicted by the UniProt CAUTION and the curated NOT|dihydropyrimidinase.
      Remove (legacy mis-annotation).
    supported_by:
    - reference_id: file:human/DPYSL2/DPYSL2-uniprot.txt
      supporting_text: Lacks most of the conserved residues that are essential for
- term:
    id: GO:0006139
    label: nucleobase-containing compound metabolic process
  evidence_type: TAS
  original_reference_id: PMID:8973361
  qualifier: involved_in
  review:
    summary: Nucleobase-containing compound metabolic process (TAS, PMID:8973361) tied to the now-defunct
      dihydropyrimidinase activity claim.
    action: REMOVE
    reason: Legacy process annotation dependent on the superseded enzymatic activity; remove, consistent
      with the NOT|pyrimidine catabolism.
    supported_by:
    - reference_id: file:human/DPYSL2/DPYSL2-uniprot.txt
      supporting_text: Lacks most of the conserved residues that are essential for
- term:
    id: GO:0007165
    label: signal transduction
  evidence_type: TAS
  original_reference_id: PMID:8973361
  qualifier: involved_in
  review:
    summary: 'signal transduction: a broader/secondary process for DPYSL2.'
    action: KEEP_AS_NON_CORE
    reason: Valid but non-core relative to the cytoskeletal-regulation function.
- term:
    id: GO:0007399
    label: nervous system development
  evidence_type: TAS
  original_reference_id: PMID:8973361
  qualifier: involved_in
  review:
    summary: 'nervous system development: a core neuronal/cytoskeletal process for the CRMP family (DPYSL2
      acts in semaphorin-driven cytoskeleton remodeling and neurite/axon development).'
    action: ACCEPT
    reason: Core biological process for a CRMP-family cytoskeletal regulator.
references:
- id: GO_REF:0000002
  title: GO annotation through association of InterPro records with GO terms
  findings: []
- id: GO_REF:0000024
  title: Manual transfer of experimentally-verified annotations to orthologs by curator judgment
  findings: []
- id: GO_REF:0000033
  title: Annotation inferences using phylogenetic trees
  findings: []
- id: GO_REF:0000044
  title: GO annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
  findings: []
- id: GO_REF:0000052
  title: GO annotation based on curation of immunofluorescence data (HPA)
  findings: []
- id: GO_REF:0000107
  title: Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl
    Compara
  findings: []
- id: GO_REF:0000120
  title: Combined Automated Annotation using Multiple IEA Methods
  findings: []
- id: file:human/DPYSL2/DPYSL2-uniprot.txt
  title: UniProt entry for DPYSL2
  findings:
  - statement: DPYSL2 lacks the metal-cofactor-binding residues required for dihydropyrimidinase activity.
    supporting_text: Lacks most of the conserved residues that are essential for
  - statement: DPYSL2 belongs to the metallo-dependent hydrolase superfamily.
    supporting_text: Belongs to the metallo-dependent hydrolases superfamily.
- id: PMID:16260607
  title: CRMP-2 is involved in kinesin-1-dependent transport of the Sra-1/WAVE1 complex and axon formation.
  findings: []
- id: PMID:19235893
  title: Protein product of CLN6 gene responsible for variant late-onset infantile neuronal ceroid lipofuscinosis
    interacts with CRMP-2.
  findings: []
- id: PMID:20458337
  title: MHC class II-associated proteins in B-cell exosomes and potential functional implications for
    exosome biogenesis.
  findings: []
- id: PMID:20801876
  title: Collapsin response mediator protein-2 (Crmp2) regulates trafficking by linking endocytic regulatory
    proteins to dynein motors.
  findings: []
- id: PMID:21516116
  title: Next-generation sequencing to generate interactome datasets.
  findings: []
- id: PMID:21900206
  title: A directed protein interaction network for investigating intracellular signal transduction.
  findings: []
- id: PMID:24722188
  title: Protein interaction network of alternatively spliced isoforms from brain links genetic risk factors
    for autism.
  findings: []
- id: PMID:25416956
  title: A proteome-scale map of the human interactome network.
  findings: []
- id: PMID:28514442
  title: Architecture of the human interactome defines protein communities and disease networks.
  findings: []
- id: PMID:29892012
  title: An interactome perturbation framework prioritizes damaging missense mutations for developmental
    disorders.
  findings: []
- id: PMID:31515488
  title: Extensive disruption of protein interactions by genetic variants across the allele frequency
    spectrum in human populations.
  findings: []
- id: PMID:32296183
  title: A reference map of the human binary protein interactome.
  findings: []
- id: PMID:32814053
  title: Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread
    Protein Aggregation in Affected Brains.
  findings: []
- id: PMID:33961781
  title: Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
  findings: []
- id: PMID:36950384
  title: Protein interaction studies in human induced neurons indicate convergent biology underlying autism
    spectrum disorders.
  findings: []
- id: PMID:40205054
  title: Multimodal cell maps as a foundation for structural and functional genomics.
  findings: []
- id: PMID:8973361
  title: A novel gene family defined by human dihydropyrimidinase and three related proteins with differential
    tissue distribution.
  findings: []
- id: Reactome:R-HSA-399944
  title: Reactome pathway (CRMP/semaphorin signalling)
  findings: []
- id: Reactome:R-HSA-399947
  title: Reactome pathway (CRMP/semaphorin signalling)
  findings: []
- id: Reactome:R-HSA-399951
  title: Reactome pathway (CRMP/semaphorin signalling)
  findings: []
- id: Reactome:R-HSA-443783
  title: Reactome pathway (CRMP/semaphorin signalling)
  findings: []
aliases:
- CRMP2
- CRMP-2
- DRP-2
- ULIP-2
- TOAD-64
- Collapsin response mediator protein 2
core_functions:
- description: Catalytically inactive CRMP-family cytoskeletal regulator that binds tubulin and promotes
    microtubule assembly to drive axon growth, neuronal polarity and semaphorin-3A-induced growth-cone
    dynamics; phosphoregulated by GSK3B/CDK5/ROCK.
  directly_involved_in:
  - id: GO:0007399
    label: nervous system development
  - id: GO:0007010
    label: cytoskeleton organization
  locations:
  - id: GO:0005829
    label: cytosol
  - id: GO:0015630
    label: microtubule cytoskeleton
  supported_by:
  - reference_id: file:human/DPYSL2/DPYSL2-uniprot.txt
    supporting_text: Lacks most of the conserved residues that are essential for
suggested_questions:
- question: How does CRMP2 (DPYSL2) select tubulin heterodimers to promote microtubule assembly, and how
    do sequential GSK3B/CDK5/ROCK phosphorylations switch this binding off during semaphorin-3A-induced
    growth-cone collapse?
suggested_experiments:
- hypothesis: Map the CRMP2 phospho-states that toggle tubulin binding.
  description: Reconstitute microtubule assembly with purified CRMP2 phospho-mimetic/phospho-dead variants
    and measure tubulin-heterodimer binding and axon outgrowth in neurons.