DRC4, also known as GAS8 or GAS11, is a coiled-coil structural protein of the nexin-dynein regulatory complex in motile cilia and flagella. It helps organize the axonemal scaffold linking neighboring microtubule doublets and coordinating ciliary bending. DRC4 also associates with microtubules and has reported accessory roles in Smoothened signaling and Golgi-associated interactions. Human DRC4 defects impair respiratory ciliary movement and can disrupt sperm flagellar structure.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0000226 microtubule cytoskeleton organization | IEA GO_REF:0000107 | ACCEPT | Summary: DRC4 participates in microtubule organization. Reason: The source maps to mouse Gas8/Q60779, for which PMID:17366626 provides experimental microtubule association and organization evidence. Human DRC4 is a conserved N-DRC scaffold, and human ciliary structural defects independently support organization of the axonemal microtubule apparatus. The term also accommodates the reported nonaxonemal microtubule context and is not restricted to a purified human assay. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:Q60779 SUPPORTS TRANSFER Mouse Gas8 is the literal donor. Its current experimental annotations and source publications were checked; organism and assay boundaries remain explicit in the annotation reason. ensembl:ENSMUSP00000090730 SUPPORTS TRANSFER The source snapshot maps this mouse translation to the same Q60779 donor. It is an orthology-mapping identifier, not a separate experimental assay. |
| GO:0000226 microtubule cytoskeleton organization | ISS GO_REF:0000024 | ACCEPT | Summary: DRC4 participates in microtubule organization. Reason: The source maps to mouse Gas8/Q60779, for which PMID:17366626 provides experimental microtubule association and organization evidence. Human DRC4 is a conserved N-DRC scaffold, and human ciliary structural defects independently support organization of the axonemal microtubule apparatus. The term also accommodates the reported nonaxonemal microtubule context and is not restricted to a purified human assay. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:Q60779 SUPPORTS TRANSFER Mouse Gas8 is the literal donor. Its current experimental annotations and source publications were checked; organism and assay boundaries remain explicit in the annotation reason. |
| GO:0003341 cilium movement | NAS PMID:37258679 Axonemal structures reveal mechanoregulatory and disease mec... | ACCEPT | Summary: The N-DRC scaffold participates in ciliary movement. Reason: The human respiratory structural inventory identifies O95995/478 in the N-DRC. Detailed scaffold geometry is resolved in algae and human homologous subcomplexes are placed in density. Human DRC4 loss reduces ciliary beating amplitude while preserving several dynein-arm markers, consistent with structural participation in movement rather than motor ATPase catalysis. |
| GO:0005515 protein binding | IPI PMID:21659505 Growth Arrest Specific 8 (Gas8) and G protein-coupled recept... | MODIFY | Summary: The human GAS8 fragments bind the Smoothened receptor. Reason: PMID:21659505 tests human GAS8 residues 357–478 and 386–478 against the human SMO C-terminal region by two-hybrid analysis. SMO is a G protein-coupled receptor, supporting this partner-class refinement. The accompanying Flag-Gas8 co-immunoprecipitation and cell perturbations use mouse Gas8, and the class term does not claim DRC4 is a receptor, agonist or kinase. Proposed replacements: G protein-coupled receptor binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive POTEB3 interaction in its screening context. Reason: The literal DRC4–POTEB3 (A0JP26) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 34, 86, 192). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed POTEB3 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive CYSRT1 interaction in its screening context. Reason: The literal DRC4–CYSRT1 (A8MQ03) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 13, 99, 219). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed CYSRT1 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified PLK4 binary interaction to protein kinase binding. Reason: The literal DRC4–PLK4 (O00444) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 11, 96, 217). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. PLK4 is a catalytically active protein kinase. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. Proposed replacements: protein kinase binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive TRAF5 interaction in its screening context. Reason: The literal DRC4–TRAF5 (O00463) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 95, 213, 333). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. TRAF5 is a signaling adaptor; its gene-family name does not establish an independently catalytic ubiquitin-ligase reagent. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified PRMT5 binary interaction to histone methyltransferase binding. Reason: The literal DRC4–PRMT5 (O14744) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 176, 236, 310). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. PRMT5 methylates histone arginine residues as well as nonhistone substrates. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. Proposed replacements: histone methyltransferase binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified ZBTB39 binary interaction to DNA-binding transcription factor binding. Reason: The literal DRC4–ZBTB39 (O15060) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 113, 226, 327). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The official partner record carries DNA-binding transcription repressor activity and cis-regulatory sequence-specific DNA binding by IBA. The binary pair and the independently established partner class support this binding refinement. It does not transfer transcriptional activity to DRC4 or establish a native regulatory interaction. Proposed replacements: DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive POLR1C interaction in its screening context. Reason: The literal DRC4–POLR1C (O15160) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 128, 168, 263). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. POLR1C is a shared polymerase subunit; binding this isolated subunit is not evidence of recognizing an assembled catalytically active RNA polymerase. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified ZBTB22 binary interaction to DNA-binding transcription factor binding. Reason: The literal DRC4–ZBTB22 (O15209) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 53, 224, 308). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The official partner record carries DNA-binding transcription factor activity by ISA, cis-regulatory binding by IBA and sequence-specific DNA binding by IDA. The binary pair and the independently established partner class support this binding refinement. It does not transfer transcriptional activity to DRC4 or establish a native regulatory interaction. Proposed replacements: DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified GSTA4 binary interaction to enzyme binding. Reason: The literal DRC4–GSTA4 (O15217) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 4, 105, 216). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. GSTA4 is a catalytic glutathione transferase. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. The captured complete enzyme-binding descendant set has no appropriate glutathione-transferase-binding child; glutathione binding would describe a different ligand. Proposed replacements: enzyme binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified KDM1A binary interaction to enzyme binding. Reason: The literal DRC4–KDM1A (O60341) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 46, 180, 285). The target and partner parent-sequence ROGIDs match the current official sequences. Separately, the assay feature records a sufficient-binding region of 123-852; the parent checksum is not a full-length reagent claim. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. KDM1A is a histone demethylase; the recorded 123–852 fragment retains the catalytic region. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. The captured complete enzyme-binding descendant set has no appropriate histone-demethylase-binding child. Histone methyltransferase binding and histone deacetylase binding describe different enzyme classes. The recorded KDM1A region 123–852 retains its catalytic region without establishing a DRC4 chromatin function. Proposed replacements: enzyme binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified SORBS3 binary interaction to cytoskeletal regulatory protein binding. Reason: The literal DRC4–SORBS3 (O60504) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 159, 242, 304). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. the displayed vinexin-alpha/SORBS3 product promotes actin stress-fiber organization. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. Proposed replacements: cytoskeletal regulatory protein binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive HSF2BP interaction in its screening context. Reason: The literal DRC4–HSF2BP (O75031) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 120, 137, 260). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. HSF2BP is a recombination-associated factor, and its name does not make it a protein-folding chaperone. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified BCAR3 binary interaction to cytoskeletal regulatory protein binding. Reason: The literal DRC4–BCAR3 (O75815) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 122, 173, 272). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. BCAR3 regulates actin redistribution and membrane ruffling; its disputed GEF activity is not used for this classification. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. Proposed replacements: cytoskeletal regulatory protein binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified KRT14 binary interaction to cytoskeletal protein binding. Reason: The literal DRC4–KRT14 (P02533) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 72, 79, 203). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. KRT14 is a keratin cytoskeletal protein. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. This does not assert binding to an assembled filament. Proposed replacements: cytoskeletal protein binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified YES1 binary interaction to protein kinase binding. Reason: The literal DRC4–YES1 (P07947) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 47, 181, 286). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. YES1 is a catalytic protein tyrosine kinase. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. Proposed replacements: protein kinase binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive ERCC1 interaction in its screening context. Reason: The literal DRC4–ERCC1 (P07992-3) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 36, 88, 194). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The exact ERCC1-3 reagent is retained; an ERCC1 interaction is not automatically binding to the catalytic ERCC4 nuclease. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified KRT16 binary interaction to cytoskeletal protein binding. Reason: The literal DRC4–KRT16 (P08779) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 114, 227, 328). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. KRT16 is a keratin cytoskeletal protein. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. This does not assert binding to an assembled filament. Proposed replacements: cytoskeletal protein binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified SKIL binary interaction to transcription coregulator binding. Reason: The literal DRC4–SKIL (P12757) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 24, 209, 324). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The authentic PMID:10531062 abstract reports SnoN association with Smad2/Smad4 and recruitment of N-CoR to repress Smad-mediated transcriptional activation. That specific corepressor mechanism supports the partner class; the paper does not assay DRC4, and its full Methods were not recovered. The binary pair and the independently established partner class support this binding refinement. It does not transfer transcriptional activity to DRC4 or establish a native regulatory interaction. Proposed replacements: transcription coregulator binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified ZNF250 binary interaction to DNA-binding transcription factor binding. Reason: The literal DRC4–ZNF250 (P15622-3) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 124, 139, 196). The target and partner parent-sequence ROGIDs match the current official sequences. Separately, the assay feature records a sufficient-binding region of 46-555; the parent checksum is not a full-length reagent claim. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The official partner record carries DNA-binding transcription factor activity by IBA and sequence-specific DNA binding by IDA. Product P15622-3 deletes residues 15–19; the separately recorded sufficient-binding region 46–555 retains the complete 13-zinc-finger array but truncates the N-terminal KRAB module. The class refinement does not require an intact KRAB repression module or assert a full-length reagent. The binary pair and the independently established partner class support this binding refinement. It does not transfer transcriptional activity to DRC4 or establish a native regulatory interaction. Proposed replacements: DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified KRT15 binary interaction to cytoskeletal protein binding. Reason: The literal DRC4–KRT15 (P19012) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 15, 92, 234). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. KRT15 is a keratin cytoskeletal protein. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. This does not assert binding to an assembled filament. Proposed replacements: cytoskeletal protein binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified TNFAIP3 binary interaction to ubiquitin protein ligase binding. Reason: The literal DRC4–TNFAIP3 (P21580) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 10, 147, 238). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. TNFAIP3 has documented ubiquitin-protein ligase activity in addition to its deubiquitinating activity. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. Proposed replacements: ubiquitin protein ligase binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive IGFBP6 interaction in its screening context. Reason: The literal DRC4–IGFBP6 (P24592) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 35, 87, 193). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed IGFBP6 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified LMO2 binary interaction to transcription coregulator binding. Reason: The literal DRC4–LMO2 (P25791-3) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 21, 212, 332). The target and partner parent-sequence ROGIDs match the current official sequences. Separately, the assay feature records a sufficient-binding region of 70-227; the parent checksum is not a full-length reagent claim. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. LMO2 is a non-DNA-binding transcriptional cofactor associated with TAL1 and LDB1; the tested P25791-3 region 70–227 retains both LIM domains. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. Proposed replacements: transcription coregulator binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified LMO1 binary interaction to transcription coregulator binding. Reason: The literal DRC4–LMO1 (P25800) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 26, 296, 338). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The official partner record carries transcription coactivator activity by IBA and two LIM domains. This supports a coregulator class without asserting direct DNA binding. The binary pair and the independently established partner class support this binding refinement. It does not transfer transcriptional activity to DRC4 or establish a native regulatory interaction. Proposed replacements: transcription coregulator binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive TXLNA interaction in its screening context. Reason: The literal DRC4–TXLNA (P40222) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 27, 66, 90). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed TXLNA record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive TSFM interaction in its screening context. Reason: The literal DRC4–TSFM (P43897) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 16, 93, 235). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. TSFM is an exchange factor for mitochondrial EF-Tu, not EF-Tu itself; no DRC4 GTPase or exchange activity follows. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified HOXA1 binary interaction to DNA-binding transcription factor binding. Reason: The literal DRC4–HOXA1 (P49639) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 45, 84, 275). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. HOXA1 is a sequence-specific DNA-binding transcription factor. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. Proposed replacements: DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive CCNG1 interaction in its screening context. Reason: The literal DRC4–CCNG1 (P51959) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 50, 126, 141). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. CCNG1 is a cyclin-associated regulator, not a catalytic protein kinase. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive CRYBB1 interaction in its screening context. Reason: The literal DRC4–CRYBB1 (P53674) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 41, 243, 298). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. CRYBB1 is a lens crystallin; structural status does not establish membership in the actin, microtubule or intermediate-filament cytoskeleton. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive GEM interaction in its screening context. Reason: The literal DRC4–GEM (P55040) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 144, 187, 199). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. GEM is a guanine-nucleotide-binding regulatory protein whose official record reports undetectable intrinsic GTPase activity; no catalytic-partner inference is required here. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive MFAP1 interaction in its screening context. Reason: The literal DRC4–MFAP1 (P55081) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 94, 228, 329). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed MFAP1 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive CKS1B interaction in its screening context. Reason: The literal DRC4–CKS1B (P61024) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 71, 78, 202). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. CKS1B is a kinase-regulatory subunit, not a catalytic kinase. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive CDR2 interaction in its screening context. Reason: The literal DRC4–CDR2 (Q01850) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 25, 210, 325). The target and partner parent-sequence ROGIDs match the current official sequences. Separately, the assay feature records a sufficient-binding region of 12-454; the parent checksum is not a full-length reagent claim. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed CDR2 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified GOLGA2 binary interaction to cytoskeletal regulatory protein binding. Reason: The literal DRC4–GOLGA2 (Q08379) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 8, 106, 221). The target and partner parent-sequence ROGIDs match the current official sequences. Separately, the assay feature records a sufficient-binding region of 13-1002; the parent checksum is not a full-length reagent claim. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. GOLGA2 regulates microtubule nucleation and capture during spindle assembly; the recorded partner region is 13–1002. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. Proposed replacements: cytoskeletal regulatory protein binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified EHHADH binary interaction to enzyme binding. Reason: The literal DRC4–EHHADH (Q08426) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 62, 80, 283). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. EHHADH catalyzes enoyl-CoA hydration and hydroxyacyl-CoA oxidation. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. The captured complete enzyme-binding descendant set has no appropriate narrower term for this enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase. Lipase binding would not describe its demonstrated catalytic class. Proposed replacements: enzyme binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified TRAF2 binary interaction to ubiquitin protein ligase binding. Reason: The literal DRC4–TRAF2 (Q12933) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 172, 257, 316). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. TRAF2 is an E3 ubiquitin-protein ligase. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. Proposed replacements: ubiquitin protein ligase binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive PPFIA1 interaction in its screening context. Reason: The literal DRC4–PPFIA1 (Q13136) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 132, 174, 267). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed PPFIA1 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive BYSL interaction in its screening context. Reason: The literal DRC4–BYSL (Q13895) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 40, 59, 291). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed BYSL record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified FHL2 binary interaction to transcription coregulator binding. Reason: The literal DRC4–FHL2 (Q14192) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 108, 215, 339). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. FHL2 modulates transcription through interactions with E4F1 and FOXO1. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. Proposed replacements: transcription coregulator binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive LASP1 interaction in its screening context. Reason: The literal DRC4–LASP1 (Q14847-2) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 133, 178, 295). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The tested LASP1-2 has a replaced C-terminal region; the general actin-regulatory description is not used to assert experimentally established activity of this particular splice product. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive ARL6IP1 interaction in its screening context. Reason: The literal DRC4–ARL6IP1 (Q15041) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 170, 270, 314). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed ARL6IP1 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive RALBP1 interaction in its screening context. Reason: The literal DRC4–RALBP1 (Q15311) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 110, 205, 320). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. RALBP1 is a signaling/GAP scaffold; the possible ATP-dependent transport description is not treated as a verified ATPase class for this pair. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive TCEA2 binary interaction without an unsupported transcription-coregulator refinement. Reason: The literal DRC4–TCEA2 (Q15560) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 44, 49, 183). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing a native interface or partner-specific orthogonal validation. TCEA2 assists RNA polymerase II elongation and recovery from transcription arrest. That activity does not by itself establish the DNA-bound transcription-factor/coregulator relationship specified by GO:0003712 and GO:0001221. Retain the measured pair as non-core; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive CCDC6 interaction in its screening context. Reason: The literal DRC4–CCDC6 (Q16204) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 162, 185, 269). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed CCDC6 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified CDC37 binary interaction to protein-folding chaperone binding. Reason: The literal DRC4–CDC37 (Q16543) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 150, 241, 303). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing a native interface or partner-specific orthogonal validation. CDC37 is a kinase-client-binding Hsp90 co-chaperone; the official functional record and the human Hsp90–CDC37–CDK4 structure in PMID:27339980 establish client contact and stabilization of an unfolded kinase intermediate. This partner fits the chaperone class in GO:0051087. The refinement describes binding to CDC37, not a DRC4 folding activity or evidence that DRC4 itself is a chaperone client. Proposed replacements: protein-folding chaperone binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive CWF19L2 interaction in its screening context. Reason: The literal DRC4–CWF19L2 (Q2TBE0) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 28, 55, 195). The target and partner parent-sequence ROGIDs match the current official sequences. Separately, the assay feature records a sufficient-binding region of 155-893; the parent checksum is not a full-length reagent claim. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed CWF19L2 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive FAM161A interaction in its screening context. Reason: The literal DRC4–FAM161A (Q3B820) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 121, 138, 261). The target and partner parent-sequence ROGIDs match the current official sequences. Separately, the assay feature records a sufficient-binding region of 110-660; the parent checksum is not a full-length reagent claim. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. FAM161A ciliogenesis association does not by itself define a specific catalytic or regulatory binding class for its recorded fragment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified ZNF792 binary interaction to DNA-binding transcription factor binding. Reason: The literal DRC4–ZNF792 (Q3KQV3) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 107, 214, 334). The target and partner parent-sequence ROGIDs match the current official sequences. Separately, the assay feature records a sufficient-binding region of 80-632; the parent checksum is not a full-length reagent claim. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The official partner record carries DNA-binding transcription factor activity and cis-regulatory binding by IBA. The recorded sufficient-binding region 80–632 retains the zinc-finger array while truncating most of the KRAB region; no intact repression module or full-length reagent is assumed. The binary pair and the independently established partner class support this binding refinement. It does not transfer transcriptional activity to DRC4 or establish a native regulatory interaction. Proposed replacements: DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive TSGA10IP interaction in its screening context. Reason: The literal DRC4–TSGA10IP (Q3SY00) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 31, 69, 290). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed TSGA10IP record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive GRIPAP1 interaction in its screening context. Reason: The literal DRC4–GRIPAP1 (Q4V328) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 18, 101, 240). The target and partner parent-sequence ROGIDs match the current official sequences. Separately, the assay feature records a sufficient-binding region of 358-841; the parent checksum is not a full-length reagent claim. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed GRIPAP1 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive CT45A1 interaction in its screening context. Reason: The literal DRC4–CT45A1 (Q5HYN5) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 143, 186, 198). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed CT45A1 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive RINT1 interaction in its screening context. Reason: The literal DRC4–RINT1 (Q6NUQ1) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 14, 98, 220). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed RINT1 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive GPRASP3 interaction in its screening context. Reason: The literal DRC4–GPRASP3 (Q6PI77) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 22, 207, 322). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed GPRASP3 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified ZNF620 binary interaction to DNA-binding transcription factor binding. Reason: The literal DRC4–ZNF620 (Q6ZNG0) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 37, 73, 91). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The official partner record carries DNA-binding transcription factor activity and regulatory-region sequence-specific DNA binding by IBA. The binary pair and the independently established partner class support this binding refinement. It does not transfer transcriptional activity to DRC4 or establish a native regulatory interaction. Proposed replacements: DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive NKAPD1 interaction in its screening context. Reason: The literal DRC4–NKAPD1 (Q6ZUT1) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 119, 136, 259). The target and partner parent-sequence ROGIDs match the current official sequences. Separately, the assay feature records a sufficient-binding region of 30-292; the parent checksum is not a full-length reagent claim. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed NKAPD1 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified ZNF572 binary interaction to DNA-binding transcription factor binding. Reason: The literal DRC4–ZNF572 (Q7Z3I7) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 19, 39, 58). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The official partner record carries DNA-binding transcription repressor activity and cis-regulatory sequence-specific DNA binding by IBA. The binary pair and the independently established partner class support this binding refinement. It does not transfer transcriptional activity to DRC4 or establish a native regulatory interaction. Proposed replacements: DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified KRT27 binary interaction to cytoskeletal protein binding. Reason: The literal DRC4–KRT27 (Q7Z3Y8) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 148, 278, 302). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. KRT27 is a keratin cytoskeletal protein. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. This does not assert binding to an assembled filament. Proposed replacements: cytoskeletal protein binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive CCDC125 interaction in its screening context. Reason: The literal DRC4–CCDC125 (Q86Z20) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 7, 154, 254). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed CCDC125 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive CCDC146 interaction in its screening context. Reason: The literal DRC4–CCDC146 (Q8IYE0) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 70, 77, 282). The target and partner parent-sequence ROGIDs match the current official sequences. Separately, the assay feature records a sufficient-binding region of 658-955; the parent checksum is not a full-length reagent claim. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The CCDC146 reagent is the 658–955 fragment; a role of the full gene in sperm formation is not a direct assay of this fragment as a structural scaffold. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive TXLNB interaction in its screening context. Reason: The literal DRC4–TXLNB (Q8N3L3) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 61, 189, 273). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed TXLNB record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive CPSF7 interaction in its screening context. Reason: The literal DRC4–CPSF7 (Q8N684-3) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 165, 256, 313). The target and partner parent-sequence ROGIDs match the current official sequences. Separately, the assay feature records a sufficient-binding region of 44-514; the parent checksum is not a full-length reagent claim. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. CPSF7-3 is a cleavage-factor subunit; the recorded 44–514 region is not evidence of binding an independently catalytic nuclease. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive USHBP1 interaction in its screening context. Reason: The literal DRC4–USHBP1 (Q8N6Y0) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 116, 135, 293). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed USHBP1 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive EIF1AD interaction in its screening context. Reason: The literal DRC4–EIF1AD (Q8N9N8) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 171, 271, 315). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed EIF1AD record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified CEP70 binary interaction to cytoskeletal regulatory protein binding. Reason: The literal DRC4–CEP70 (Q8NHQ1) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 43, 245, 300). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. CEP70 regulates interphase microtubules and mitotic spindle organization. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. Proposed replacements: cytoskeletal regulatory protein binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive GADD45GIP1 interaction in its screening context. Reason: The literal DRC4–GADD45GIP1 (Q8TAE8) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 51, 85, 274). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed GADD45GIP1 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive SPATA18 interaction in its screening context. Reason: The literal DRC4–SPATA18 (Q8TC71) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 63, 188, 281). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed SPATA18 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive CCHCR1 interaction in its screening context. Reason: The literal DRC4–CCHCR1 (Q8TD31-3) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 30, 68, 288). The target and partner parent-sequence ROGIDs match the current official sequences. Separately, the assay feature records a sufficient-binding region of 90-835; the parent checksum is not a full-length reagent claim. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed CCHCR1 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified SP7 binary interaction to DNA-binding transcription factor binding. Reason: The literal DRC4–SP7 (Q8TDD2) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 65, 75, 201). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. SP7 is a sequence-specific DNA-binding transcription factor. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. Proposed replacements: DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive TRIM41 interaction in its screening context. Reason: The literal DRC4–TRIM41 (Q8WV44) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 5, 158, 276). The target and partner parent-sequence ROGIDs match the current official sequences. Separately, the assay feature records a sufficient-binding region of 86-630; the parent checksum is not a full-length reagent claim. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The TRIM41 parent sequence is authenticated, but the recorded sufficient-binding region 86–630 excludes its annotated RING region 20–61. The result is not described as an assay of an intact active E3 ligase. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive RUBCN interaction in its screening context. Reason: The literal DRC4–RUBCN (Q92622) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 117, 142, 197). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. RUBCN regulates an enzyme-containing autophagy complex but is not itself identified here as that catalytic enzyme. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified DDX17 binary interaction to ATPase binding. Reason: The literal DRC4–DDX17 (Q92841) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 42, 244, 299). The target and partner parent-sequence ROGIDs match the current official sequences. Separately, the assay feature records a sufficient-binding region of 80-729; the parent checksum is not a full-length reagent claim. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. DDX17 is an ATP-hydrolyzing RNA helicase; the recorded 80–729 region retains the helicase catalytic domains. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. Proposed replacements: ATPase binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive L3MBTL2 interaction in its screening context. Reason: The literal DRC4–L3MBTL2 (Q969R5) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 130, 164, 265). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The official L3MBTL2 record gives chromatin-reader activity and a putative regulatory role; no definite transcription-coregulator subclass is imposed on this pair. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive EVI5L interaction in its screening context. Reason: The literal DRC4–EVI5L (Q96CN4) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 9, 177, 237). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. EVI5L stimulates another protein’s GTPase activity as a GAP; that is not an assay of DRC4 catalysis or a catalytic GTPase partner. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive GCC1 interaction in its screening context. Reason: The literal DRC4–GCC1 (Q96CN9) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 111, 206, 321). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed GCC1 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive BORCS6 interaction in its screening context. Reason: The literal DRC4–BORCS6 (Q96GS4) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 52, 223, 309). The target and partner parent-sequence ROGIDs match the current official sequences. Separately, the assay feature records a sufficient-binding region of 135-357; the parent checksum is not a full-length reagent claim. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. BORCS6 is a lysosomal trafficking complex subunit, and the recorded 135–357 fragment does not establish binding to a complete motor. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive MRFAP1L1 interaction in its screening context. Reason: The literal DRC4–MRFAP1L1 (Q96HT8) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 12, 97, 218). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed MRFAP1L1 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive EAF1 binary interaction without an unsupported transcription-coregulator refinement. Reason: The literal DRC4–EAF1 (Q96JC9) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 23, 208, 323). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing a native interface or partner-specific orthogonal validation. EAF1 stimulates ELL/ELL2 elongation activity. An elongation cofactor is not automatically a transcription coregulator under GO:0003712 and GO:0001221, which describe regulation through DNA-bound transcription factors and the basal machinery. Retain the measured pair as non-core; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive L3MBTL3 interaction in its screening context. Reason: The literal DRC4–L3MBTL3 (Q96JM7-2) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 153, 248, 306). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The exact L3MBTL3-2 parent sequence and its 72–96 deletion are retained; the general transcriptional function is not promoted to an experimentally established function of this splice reagent. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified CFAP53 binary interaction to cytoskeletal protein binding. Reason: The literal DRC4–CFAP53 (Q96M91) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 57, 83, 191). The target and partner parent-sequence ROGIDs match the current official sequences. Separately, the assay feature records a sufficient-binding region of 116-514; the parent checksum is not a full-length reagent claim. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. CFAP53 is a microtubule inner protein in axonemal doublets; the assay records its 116–514 region. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. This does not assert binding to an assembled filament. Proposed replacements: cytoskeletal protein binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive STAC3 interaction in its screening context. Reason: The literal DRC4–STAC3 (Q96MF2) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 125, 140, 294). The target and partner parent-sequence ROGIDs match the current official sequences. Separately, the assay feature records a sufficient-binding region of 40-364; the parent checksum is not a full-length reagent claim. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The STAC3 40–364 region comes from a calcium-channel regulatory protein, not a calcium channel itself. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive HEXIM2 binary interaction without an unsupported transcription-coregulator refinement. Reason: The literal DRC4–HEXIM2 (Q96MH2) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 146, 231, 336). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing a native interface or partner-specific orthogonal validation. HEXIM2 sequesters and inhibits P-TEFb in the 7SK ribonucleoprotein complex. This elongation-control role does not establish the DNA-bound transcription-factor/coregulator relationship required for GO:0001221; an inhibitor is also not the kinase it inhibits. Retain the measured pair as non-core; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive FAM161B interaction in its screening context. Reason: The literal DRC4–FAM161B (Q96MY7) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 33, 76, 89). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed FAM161B record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive PNMA5 interaction in its screening context. Reason: The literal DRC4–PNMA5 (Q96PV4) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 123, 166, 258). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed PNMA5 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified PNKP interaction to kinase and phosphatase binding. Reason: The literal DRC4–PNKP (Q96T60) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 48, 182, 287). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. PNKP is a catalytic DNA-end kinase and phosphatase, not a protein kinase. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. Current GO:0019900 and GO:0019902 describe the two demonstrated catalytic partner classes. The protein-kinase and protein-phosphatase children would be incorrect for PNKP DNA-end chemistry; no domain-specific interface or modulation by DRC4 is inferred. Proposed replacements: kinase binding phosphatase binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive PRPF18 interaction in its screening context. Reason: The literal DRC4–PRPF18 (Q99633) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 160, 246, 311). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed PRPF18 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive MMTAG2 interaction in its screening context. Reason: The literal DRC4–MMTAG2 (Q9BU76) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 102, 232, 337). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed MMTAG2 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive CINP interaction in its screening context. Reason: The literal DRC4–CINP (Q9BW66) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 64, 179, 292). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. CINP is a component of the 55LCC ATPase complex; complex-level ATPase activity is not automatically activity of the isolated CINP partner. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive YJU2 interaction in its screening context. Reason: The literal DRC4–YJU2 (Q9BW85) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 118, 169, 264). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. YJU2 stabilizes spliceosome architecture; it is not itself the catalytic RNA component of the spliceosome. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive SCNM1 interaction in its screening context. Reason: The literal DRC4–SCNM1 (Q9BWG6) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 67, 81, 284). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed SCNM1 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive CARD10 interaction in its screening context. Reason: The literal DRC4–CARD10 (Q9BWT7) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 161, 247, 312). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed CARD10 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive TRIM54 interaction in its screening context. Reason: The literal DRC4–TRIM54 (Q9BYV2) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 109, 204, 319). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The official TRIM54 function is a qualified microtubule-stabilization hypothesis; no catalytic class is assigned solely from the TRIM family name. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified PRDM14 binary interaction to DNA-binding transcription factor binding. Reason: The literal DRC4–PRDM14 (Q9GZV8) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 60, 74, 279). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. PRDM14 binds a defined DNA consensus sequence and regulates transcription. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. Proposed replacements: DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive CARD9 interaction in its screening context. Reason: The literal DRC4–CARD9 (Q9H257-2) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 54, 253, 297). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The exact CARD9-2 signaling-adaptor reagent is retained without assigning DRC4 an immune-signaling activity. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified ZBTB26 binary interaction to DNA-binding transcription factor binding. Reason: The literal DRC4–ZBTB26 (Q9HCK0) pair is present in 5 IntAct records from PMID:32296183 (saved physical lines 29, 115, 134, 222, 289). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The official partner record carries DNA-binding transcription repressor activity by IBA and sequence-specific DNA binding by IDA. Five literal pair records, rather than three, support the interaction identity. The binary pair and the independently established partner class support this binding refinement. It does not transfer transcriptional activity to DRC4 or establish a native regulatory interaction. Proposed replacements: DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified DMAP1 binary interaction to transcription coregulator binding. Reason: The literal DRC4–DMAP1 (Q9NPF5) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 56, 82, 190). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. DMAP1 is a transcriptional coregulator associated with transcription-repression and NuA4 complexes, not an independently catalytic acetyltransferase. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. Proposed replacements: transcription coregulator binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive DISC1 interaction in its screening context. Reason: The literal DRC4–DISC1 (Q9NRI5-2) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 156, 249, 307). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. DISC1-2 has a distinct splice deletion and broad scaffold functions; no untested equivalence of its cellular activities is inferred. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive HOMER3 interaction in its screening context. Reason: The literal DRC4–HOMER3 (Q9NSC5) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 20, 211, 331). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. HOMER3 is a postsynaptic scaffold rather than one of the receptors or enzymes that it recruits. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified THAP1 binary interaction to DNA-binding transcription factor binding. Reason: The literal DRC4–THAP1 (Q9NVV9) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 3, 103, 233). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. THAP1 binds a defined DNA motif and regulates target transcription. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. Proposed replacements: DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified RBAK binary interaction to DNA-binding transcription factor binding. Reason: The literal DRC4–RBAK (Q9NYW8) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 112, 225, 326). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The official partner record carries DNA-binding transcription factor activity and cis-regulatory binding by IBA, with reviewed transcriptional regulation. The binary pair and the independently established partner class support this binding refinement. It does not transfer transcriptional activity to DRC4 or establish a native regulatory interaction. Proposed replacements: DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive SPG21 interaction in its screening context. Reason: The literal DRC4–SPG21 (Q9NZD8) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 32, 38, 200). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed SPG21 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified LHX3 binary interaction to DNA-binding transcription factor binding. Reason: The literal DRC4–LHX3 (Q9UBR4-2) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 131, 175, 268). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. LHX3 is a sequence-specific DNA-binding transcription factor; Q9UBR4-2 changes only its N-terminal segment and retains the homeodomain. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. Proposed replacements: DNA-binding transcription factor binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive ZNF212 interaction in its screening context. Reason: The literal DRC4–ZNF212 (Q9UDV6) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 155, 251, 317). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed ZNF212 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive ZNF629 interaction in its screening context. Reason: The literal DRC4–ZNF629 (Q9UEG4) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 149, 277, 301). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. Current official QuickGO records include cis-regulatory-region binding (GO:0000976, IBA) but no specific DNA-binding transcription-factor molecular function. The broader transcription-regulation inference and C2H2 domains do not resolve the stricter partner class. Retain the positive pair as non-core; this uncertainty is not evidence of biological inactivity. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified DAXX binary interaction to transcription coregulator binding. Reason: The literal DRC4–DAXX (Q9UER7) pair is present in 5 IntAct records from PMID:32296183 (saved physical lines 151, 184, 252, 280, 318). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. DAXX is a transcription corepressor that contacts transcription factors; its histone-deposition activity is not equated with protein-folding chaperone activity. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. Proposed replacements: transcription coregulator binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified NDOR1 binary interaction to enzyme binding. Reason: The literal DRC4–NDOR1 (Q9UHB4) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 152, 255, 305). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. NDOR1 is an NADPH-dependent reductase with an experimentally supported electron-transfer reaction. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. The captured complete enzyme-binding descendant set has no appropriate narrower term for this diflavin oxidoreductase; nitric-oxide-synthase binding would assert a different enzyme class. Proposed replacements: enzyme binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: Refine the verified MID2 binary interaction to ubiquitin protein ligase binding. Reason: The literal DRC4–MID2 (Q9UJV3-2) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 145, 230, 335). The target and partner parent-sequence ROGIDs match the current official sequences. Separately, the assay feature records a sufficient-binding region of 21-705; the parent checksum is not a full-length reagent claim. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. MID2 is an E3 ubiquitin-protein ligase; the Q9UJV3-2 region 21–705 retains the complete annotated RING domain at 30–80 despite the separate downstream splice deletion. The proposed term describes the verified partner class only; it does not transfer that partner’s activity or process to DRC4. Proposed replacements: ubiquitin protein ligase binding |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive LZTS1 interaction in its screening context. Reason: The literal DRC4–LZTS1 (Q9Y250) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 17, 100, 239). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. The reviewed LZTS1 record does not establish a more informative, sufficiently resolved binding subclass for this experiment. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive COG6 interaction in its screening context. Reason: The literal DRC4–COG6 (Q9Y2V7) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 104, 229, 330). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. COG6 is a Golgi tethering-complex subunit; binding it alone does not demonstrate a DRC4 Golgi-tether mechanism. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: Retain the positive MAD1L1 interaction in its screening context. Reason: The literal DRC4–MAD1L1 (Q9Y6D9) pair is present in 3 IntAct records from PMID:32296183 (saved physical lines 129, 163, 266). The target and partner parent-sequence ROGIDs match the current official sequences. No sufficient-binding-region truncation is recorded for this pair in the selected records. HuRI pairwise retesting and sequence confirmation support a binary interaction, without establishing endogenous co-localization, a physiological interface or partner-specific orthogonal validation. MAD1L1 is a spindle-checkpoint scaffold rather than a motor or catalytic kinase. Retain the positive pair as non-core under the project binding policy; this is not a false-positive or biological noninteraction assertion. |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | MODIFY | Summary: Refine the KRT27 association using independent binary-pair support. Reason: The exact BioPlex record is a spoke-expanded anti-tag co-immunoprecipitation association, not purified direct contact. Independently, the same DRC4–KRT27 pair has verified HuRI binary records with authenticated parent sequences. KRT27 is a keratin cytoskeletal protein, so gene-level cytoskeletal protein binding is supported without claiming that BioPlex itself proves direct contact or that either experiment tests an assembled intermediate filament. Proposed replacements: cytoskeletal protein binding |
| GO:0005515 protein binding | IPI PMID:34169321 Loss of DRC1 function leads to multiple morphological abnorm... | KEEP AS NON CORE | Summary: Retain the positive DRC1–DRC4 association within the structural complex. Reason: The recovered PMID:34169321 Figure 4A and Methods show HA-DRC1 association with Flag-DRC4 in HEK293T cells. The introduced ORF species and exact sequences were not independently authenticated, and the host does not establish them. N-DRC structural evidence independently supports coassembly, but the co-immunoprecipitation is not a purified binding-interface assay. Retain the positive association while representing the established scaffold activity in the integrated core. |
| GO:0005737 cytoplasm | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: Retain the conserved cytoplasmic pool as non-core. Reason: The literal mouse Gas8/Q60779 donor has positive cytoplasm EXP/IDA annotations, including the source records for PMID:11751847 and PMID:21659505. The conserved cytoplasmic pool is therefore retained at the original resolution. The human HPA record independently reports supported, nonexclusive cytosolic ICC/IF staining with antibody HPA041311 in A-431, U-251MG and U2OS, with additional ASC52telo staining. That human observation does not change the compartment measured by the historical donor or establish fractionation, isoform specificity or absence of Golgi and ciliary pools. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: UniProtKB:Q60779 SUPPORTS TRANSFER Mouse Gas8 is the literal donor. Its current experimental annotations and source publications were checked; organism and assay boundaries remain explicit in the annotation reason. ensembl:ENSMUSP00000090730 SUPPORTS TRANSFER The source snapshot maps this mouse translation to the same Q60779 donor. It is an orthology-mapping identifier, not a separate experimental assay. UniProtKB-SubCell:SL-0086 SUPPORTS TRANSFER The original UniProt cytoplasm mapping is preserved at its own resolution. The separate human HPA cytosolic pool is nonexclusive corroboration, not the source of this mapping. Supporting Evidence: UniProtKB:Q60779 https://www.proteinatlas.org/ENSG00000141013-GAS8/subcellular |
| GO:0005737 cytoplasm | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Retain the conserved cytoplasmic pool as non-core. Reason: The literal mouse Gas8/Q60779 donor has positive cytoplasm EXP/IDA annotations, including the source records for PMID:11751847 and PMID:21659505. The conserved cytoplasmic pool is therefore retained at the original resolution. The human HPA record independently reports supported, nonexclusive cytosolic ICC/IF staining with antibody HPA041311 in A-431, U-251MG and U2OS, with additional ASC52telo staining. That human observation does not change the compartment measured by the historical donor or establish fractionation, isoform specificity or absence of Golgi and ciliary pools. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: UniProtKB:Q60779 SUPPORTS TRANSFER Mouse Gas8 is the literal donor. Its current experimental annotations and source publications were checked; organism and assay boundaries remain explicit in the annotation reason. Supporting Evidence: UniProtKB:Q60779 https://www.proteinatlas.org/ENSG00000141013-GAS8/subcellular |
| GO:0005794 Golgi apparatus | EXP PMID:16643277 Expanding the role of the dynein regulatory complex to non-a... | KEEP AS NON CORE | Summary: Retain the positive accessory Golgi pool. Reason: The original PMID:16643277 reports Golgi-associated staining in COS7 cells and human-cell/airway protein detection; its purified membrane-binding reagent is mouse Gas8 and the isolated Golgi membranes are rodent. Human HPA ICC/IF independently supports Golgi staining in ciliated cell lines. The mouse Rab3B study supplies an additional Golgi-associated context. These are positive localization observations, without a demonstrated DRC4 Golgi-tether or transport reaction. Supporting Evidence: GO_REF:0000052 https://www.proteinatlas.org/ENSG00000141013-GAS8/subcellular |
| GO:0005794 Golgi apparatus | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: Retain the positive accessory Golgi pool. Reason: The original PMID:16643277 reports Golgi-associated staining in COS7 cells and human-cell/airway protein detection; its purified membrane-binding reagent is mouse Gas8 and the isolated Golgi membranes are rodent. Human HPA ICC/IF independently supports Golgi staining in ciliated cell lines. The mouse Rab3B study supplies an additional Golgi-associated context. These are positive localization observations, without a demonstrated DRC4 Golgi-tether or transport reaction. The literal PAINT IBD at PTN000774884 includes target experimental grounding and mouse evidence; the target descends from that node with no recorded IRD on the path. Target self-evidence is not circular. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: PANTHER:PTN000774884 SUPPORTS TRANSFER The literal IBD is present for this term and node in the preserved PAINT source, and the target descends from that node. Experimental descendant contexts are recorded in the portable source extract. The target among descendants is not circular evidence. Supporting Evidence: GO_REF:0000052 https://www.proteinatlas.org/ENSG00000141013-GAS8/subcellular |
| GO:0005794 Golgi apparatus | IDA GO_REF:0000052 | KEEP AS NON CORE | Summary: Retain the positive accessory Golgi pool. Reason: The original PMID:16643277 reports Golgi-associated staining in COS7 cells and human-cell/airway protein detection; its purified membrane-binding reagent is mouse Gas8 and the isolated Golgi membranes are rodent. Human HPA ICC/IF independently supports Golgi staining in ciliated cell lines. The mouse Rab3B study supplies an additional Golgi-associated context. These are positive localization observations, without a demonstrated DRC4 Golgi-tether or transport reaction. Supporting Evidence: GO_REF:0000052 https://www.proteinatlas.org/ENSG00000141013-GAS8/subcellular |
| GO:0005794 Golgi apparatus | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: Retain the positive accessory Golgi pool. Reason: The original PMID:16643277 reports Golgi-associated staining in COS7 cells and human-cell/airway protein detection; its purified membrane-binding reagent is mouse Gas8 and the isolated Golgi membranes are rodent. Human HPA ICC/IF independently supports Golgi staining in ciliated cell lines. The mouse Rab3B study supplies an additional Golgi-associated context. These are positive localization observations, without a demonstrated DRC4 Golgi-tether or transport reaction. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: UniProtKB:Q60779 SUPPORTS TRANSFER Mouse Gas8 is the literal donor. Its current experimental annotations and source publications were checked; organism and assay boundaries remain explicit in the annotation reason. ensembl:ENSMUSP00000090730 SUPPORTS TRANSFER The source snapshot maps this mouse translation to the same Q60779 donor. It is an orthology-mapping identifier, not a separate experimental assay. UniProtKB-SubCell:SL-0132 SUPPORTS TRANSFER The original UniProt subcellular mapping is preserved. The named location and any narrower human refinement are evaluated separately in the annotation reason; nonexclusive pools remain distinct. Supporting Evidence: GO_REF:0000052 https://www.proteinatlas.org/ENSG00000141013-GAS8/subcellular |
| GO:0005856 cytoskeleton | IEA GO_REF:0000044 | MODIFY | Summary: Specify the microtubule-associated cytoskeleton. Reason: The subcellular-location mapping is broadly correct, while human microtubule-associated fragments and the conserved axonemal scaffold give the more informative microtubule cytoskeleton term. This does not imply actin or intermediate-filament structural membership. Propagation Review Root cause: TERM SCOPING PROBLEM Failure modes: GRANULARITY MISMATCH Sources checked: UniProtKB-SubCell:SL-0090 SUPPORTS TRANSFER The original UniProt subcellular mapping is preserved. The named location and any narrower human refinement are evaluated separately in the annotation reason; nonexclusive pools remain distinct. Proposed replacements: microtubule cytoskeleton |
| GO:0005874 microtubule | IBA GO_REF:0000033 | ACCEPT | Summary: Retain microtubule association. Reason: The PMID:10969087 abstract explicitly reports that a 144-residue human GAS11 domain fused to GFP associates with microtubules in mammalian cells, despite the title foregrounding a trypanosome protein. The later original PMID:16643277 independently supports microtubule-associated localization. Full Methods for the 2000 fragment were not recovered, so no exact natural isoform or native full-length contact is asserted. The literal PTN000774884 IBD uses the human experimental annotation; descent is verified and target self-grounding is expected. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000774884 SUPPORTS TRANSFER The literal IBD is present for this term and node in the preserved PAINT source, and the target descends from that node. Experimental descendant contexts are recorded in the portable source extract. The target among descendants is not circular evidence. |
| GO:0005874 microtubule | IDA PMID:10969087 T lymphocyte-triggering factor of african trypanosomes is as... | ACCEPT | Summary: Retain microtubule association. Reason: The PMID:10969087 abstract explicitly reports that a 144-residue human GAS11 domain fused to GFP associates with microtubules in mammalian cells, despite the title foregrounding a trypanosome protein. The later original PMID:16643277 independently supports microtubule-associated localization. Full Methods for the 2000 fragment were not recovered, so no exact natural isoform or native full-length contact is asserted. |
| GO:0005886 plasma membrane | IDA GO_REF:0000052 | KEEP AS NON CORE | Summary: Retain the supported human plasma-membrane-associated pool. Reason: HPA041311 ICC/IF assigns supported plasma-membrane staining in A-431, U-251MG and U2OS. This is positive nonexclusive localization evidence; lack of a transmembrane segment does not refute peripheral association. It does not establish an integral membrane topology or a separate membrane receptor function. Supporting Evidence: GO_REF:0000052 https://www.proteinatlas.org/ENSG00000141013-GAS8/subcellular |
| GO:0005929 cilium | IDA GO_REF:0000052 | KEEP AS NON CORE | Summary: Retain nonexclusive ciliary localization. Reason: DRC4 has both a core motile-axoneme pool and an accessory primary-cilium/basal-body context. Human HPA detects primary-cilium staining in RPTEC/TERT1; PMID:16643277 localizes Gas8 to the base of primary cilia in mouse mIMCD3 cells, and PMID:21659505 supplies a separate mouse signaling context. Retain the broad cilium assertion as non-core rather than narrowing every source to a motile cilium and losing the nonmotile pool. Supporting Evidence: GO_REF:0000052 https://www.proteinatlas.org/ENSG00000141013-GAS8/subcellular |
| GO:0005929 cilium | IDA PMID:16643277 Expanding the role of the dynein regulatory complex to non-a... | KEEP AS NON CORE | Summary: Retain nonexclusive ciliary localization. Reason: DRC4 has both a core motile-axoneme pool and an accessory primary-cilium/basal-body context. Human HPA detects primary-cilium staining in RPTEC/TERT1; PMID:16643277 localizes Gas8 to the base of primary cilia in mouse mIMCD3 cells, and PMID:21659505 supplies a separate mouse signaling context. Retain the broad cilium assertion as non-core rather than narrowing every source to a motile cilium and losing the nonmotile pool. Supporting Evidence: GO_REF:0000052 https://www.proteinatlas.org/ENSG00000141013-GAS8/subcellular |
| GO:0005929 cilium | IDA PMID:27120127 Mutations in GAS8, a Gene Encoding a Nexin-Dynein Regulatory... | KEEP AS NON CORE | Summary: Retain nonexclusive ciliary localization. Reason: DRC4 has both a core motile-axoneme pool and an accessory primary-cilium/basal-body context. Human HPA detects primary-cilium staining in RPTEC/TERT1; PMID:16643277 localizes Gas8 to the base of primary cilia in mouse mIMCD3 cells, and PMID:21659505 supplies a separate mouse signaling context. Retain the broad cilium assertion as non-core rather than narrowing every source to a motile cilium and losing the nonmotile pool. PMID:27120127 remains abstract-only after bounded recovery attempts; its curated positive localization is retained with independent gene-level corroboration, without inventing its imaging Methods. Supporting Evidence: GO_REF:0000052 https://www.proteinatlas.org/ENSG00000141013-GAS8/subcellular |
| GO:0005929 cilium | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: Retain nonexclusive ciliary localization. Reason: DRC4 has both a core motile-axoneme pool and an accessory primary-cilium/basal-body context. Human HPA detects primary-cilium staining in RPTEC/TERT1; PMID:16643277 localizes Gas8 to the base of primary cilia in mouse mIMCD3 cells, and PMID:21659505 supplies a separate mouse signaling context. Retain the broad cilium assertion as non-core rather than narrowing every source to a motile cilium and losing the nonmotile pool. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: UniProtKB-SubCell:SL-0066 SUPPORTS TRANSFER The original UniProt subcellular mapping is preserved. The named location and any narrower human refinement are evaluated separately in the annotation reason; nonexclusive pools remain distinct. Supporting Evidence: GO_REF:0000052 https://www.proteinatlas.org/ENSG00000141013-GAS8/subcellular |
| GO:0005930 axoneme | IDA PMID:16643277 Expanding the role of the dynein regulatory complex to non-a... | ACCEPT | Summary: Retain axonemal localization or activity as an anatomical assertion. Reason: The original source establishes an axonemal pool, and human respiratory localization, loss-of-function evidence and the structural inventory independently corroborate it. Preserve the original located_in or is_active_in qualifier. Anatomical localization is distinct from part_of membership in the N-DRC complex; the separately retained GO:0005931 source row represents that complex membership. None of these location assertions implies motor ATPase activity or universal loss of dynein arms. |
| GO:0005930 axoneme | IDA PMID:26387594 Loss-of-Function GAS8 Mutations Cause Primary Ciliary Dyskin... | ACCEPT | Summary: Retain axonemal localization or activity as an anatomical assertion. Reason: The original source establishes an axonemal pool, and human respiratory localization, loss-of-function evidence and the structural inventory independently corroborate it. Preserve the original located_in or is_active_in qualifier. Anatomical localization is distinct from part_of membership in the N-DRC complex; the separately retained GO:0005931 source row represents that complex membership. None of these location assertions implies motor ATPase activity or universal loss of dynein arms. |
| GO:0005930 axoneme | IEA GO_REF:0000044 | ACCEPT | Summary: Retain axonemal localization or activity as an anatomical assertion. Reason: The original source establishes an axonemal pool, and human respiratory localization, loss-of-function evidence and the structural inventory independently corroborate it. Preserve the original located_in or is_active_in qualifier. Anatomical localization is distinct from part_of membership in the N-DRC complex; the separately retained GO:0005931 source row represents that complex membership. None of these location assertions implies motor ATPase activity or universal loss of dynein arms. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB-SubCell:SL-0304 SUPPORTS TRANSFER The original UniProt subcellular mapping is preserved. The named location and any narrower human refinement are evaluated separately in the annotation reason; nonexclusive pools remain distinct. |
| GO:0005930 axoneme | IEP PMID:26387594 Loss-of-Function GAS8 Mutations Cause Primary Ciliary Dyskin... | ACCEPT | Summary: Retain axonemal localization or activity as an anatomical assertion. Reason: The original source establishes an axonemal pool, and human respiratory localization, loss-of-function evidence and the structural inventory independently corroborate it. Preserve the original located_in or is_active_in qualifier. Anatomical localization is distinct from part_of membership in the N-DRC complex; the separately retained GO:0005931 source row represents that complex membership. None of these location assertions implies motor ATPase activity or universal loss of dynein arms. |
| GO:0005930 axoneme | IMP PMID:26387594 Loss-of-Function GAS8 Mutations Cause Primary Ciliary Dyskin... | ACCEPT | Summary: Retain axonemal localization or activity as an anatomical assertion. Reason: The original source establishes an axonemal pool, and human respiratory localization, loss-of-function evidence and the structural inventory independently corroborate it. Preserve the original located_in or is_active_in qualifier. Anatomical localization is distinct from part_of membership in the N-DRC complex; the separately retained GO:0005931 source row represents that complex membership. None of these location assertions implies motor ATPase activity or universal loss of dynein arms. |
| GO:0005930 axoneme | NAS PMID:37258679 Axonemal structures reveal mechanoregulatory and disease mec... | ACCEPT | Summary: Retain axonemal localization or activity as an anatomical assertion. Reason: The original source establishes an axonemal pool, and human respiratory localization, loss-of-function evidence and the structural inventory independently corroborate it. Preserve the original located_in or is_active_in qualifier. Anatomical localization is distinct from part_of membership in the N-DRC complex; the separately retained GO:0005931 source row represents that complex membership. None of these location assertions implies motor ATPase activity or universal loss of dynein arms. |
| GO:0005931 axonemal nexin link | NAS PMID:37258679 Axonemal structures reveal mechanoregulatory and disease mec... | ACCEPT | Summary: DRC4 is an N-DRC structural subunit. Reason: Human Supplementary Table 3 of PMID:37258679 identifies O95995/478 as DRC4 in the nexin-dynein regulatory complex. Detailed coiled-coil geometry is algal, whereas the human reconstruction places homologous predicted subcomplexes in density. GO:0005931 describes interdoublet protein links and is distinct from the motor-containing axonemal dynein complex. |
| GO:0008017 microtubule binding | IEA GO_REF:0000120 | ACCEPT | Summary: Retain conserved microtubule-binding activity. Reason: The literal mouse donor Q60779 has IDA microtubule binding from PMID:17366626, whose authentic abstract describes single-particle binding and GMAD/IMAD effects. Human GAS11 fragment localization and the conserved N-DRC scaffold independently corroborate microtubule association. The 2007 full construct Methods remain unavailable, so this is not presented as a purified assay of a specified current human splice product. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:Q60779 SUPPORTS TRANSFER Mouse Gas8 is the literal donor. Its current experimental annotations and source publications were checked; organism and assay boundaries remain explicit in the annotation reason. ensembl:ENSMUSP00000090730 SUPPORTS TRANSFER The source snapshot maps this mouse translation to the same Q60779 donor. It is an orthology-mapping identifier, not a separate experimental assay. InterPro:IPR039308 SUPPORTS TRANSFER This domain/family mapping is a computational source. Transfer is corroborated by the independently examined mouse and human evidence described in the reason; the mapping is not presented as direct chemistry. |
| GO:0008285 negative regulation of cell population proliferation | TAS PMID:9790751 Characterization and screening for mutations of the growth a... | UNDECIDED | Summary: The historical proliferation assertion cannot be fully resolved from the accessible source. Reason: PMID:9790751 is the correct human GAS11 characterization paper. Its accessible abstract describes growth-arrest-associated expression of the mouse homolog and a tumor-suppressor candidate investigation, but does not expose a direct proliferation-regulation assay. Bounded publisher and institutional routes did not recover the full original. This does not show that the curator’s statement is false or that no such assay exists; retain the source and mark the claim unresolved. Supporting Evidence: |
| GO:0015630 microtubule cytoskeleton | IEA GO_REF:0000107 | ACCEPT | Summary: Retain microtubule-cytoskeleton localization. Reason: The mouse source and human GAS11 fragment evidence support a microtubule-associated pool beyond only the axonemal N-DRC. This appropriately specific term retains that positive broader localization without assigning actin or intermediate-filament function. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:Q60779 SUPPORTS TRANSFER Mouse Gas8 is the literal donor. Its current experimental annotations and source publications were checked; organism and assay boundaries remain explicit in the annotation reason. ensembl:ENSMUSP00000090730 SUPPORTS TRANSFER The source snapshot maps this mouse translation to the same Q60779 donor. It is an orthology-mapping identifier, not a separate experimental assay. |
| GO:0030317 flagellated sperm motility | IBA GO_REF:0000033 | ACCEPT | Summary: Retain structural participation in flagellated sperm motility. Reason: The conserved N-DRC scaffold performs structural work required for flagellar bending. The literal mouse donor includes sperm localization and expression evidence; the PAINT assertion at PTN002834877 is an inherited phylogenetic judgment, not a donor-count argument. Independently, PMID:37950557 reports a human biallelic DRC4 splice-site variant with immotile, structurally abnormal sperm. Splicing is predicted rather than RNA-confirmed, and C-terminal antibody loss allows absence or truncation. These limits do not turn DRC4 into a passive substrate of motility. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN002834877 SUPPORTS TRANSFER The literal IBD is present for this term and node in the preserved PAINT source, and the target descends from that node. Experimental descendant contexts are recorded in the portable source extract. The target among descendants is not circular evidence. |
| GO:0030317 flagellated sperm motility | IEA GO_REF:0000107 | ACCEPT | Summary: Retain structural participation in flagellated sperm motility. Reason: The conserved N-DRC scaffold performs structural work required for flagellar bending. The literal mouse donor includes sperm localization and expression evidence; the PAINT assertion at PTN002834877 is an inherited phylogenetic judgment, not a donor-count argument. Independently, PMID:37950557 reports a human biallelic DRC4 splice-site variant with immotile, structurally abnormal sperm. Splicing is predicted rather than RNA-confirmed, and C-terminal antibody loss allows absence or truncation. These limits do not turn DRC4 into a passive substrate of motility. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:Q60779 SUPPORTS TRANSFER Mouse Gas8 is the literal donor. Its current experimental annotations and source publications were checked; organism and assay boundaries remain explicit in the annotation reason. ensembl:ENSMUSP00000090730 SUPPORTS TRANSFER The source snapshot maps this mouse translation to the same Q60779 donor. It is an orthology-mapping identifier, not a separate experimental assay. |
| GO:0030317 flagellated sperm motility | ISS GO_REF:0000024 | ACCEPT | Summary: Retain structural participation in flagellated sperm motility. Reason: The conserved N-DRC scaffold performs structural work required for flagellar bending. The literal mouse donor includes sperm localization and expression evidence; the PAINT assertion at PTN002834877 is an inherited phylogenetic judgment, not a donor-count argument. Independently, PMID:37950557 reports a human biallelic DRC4 splice-site variant with immotile, structurally abnormal sperm. Splicing is predicted rather than RNA-confirmed, and C-terminal antibody loss allows absence or truncation. These limits do not turn DRC4 into a passive substrate of motility. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:Q60779 SUPPORTS TRANSFER Mouse Gas8 is the literal donor. Its current experimental annotations and source publications were checked; organism and assay boundaries remain explicit in the annotation reason. |
| GO:0030317 flagellated sperm motility | NAS PMID:37258679 Axonemal structures reveal mechanoregulatory and disease mec... | ACCEPT | Summary: Retain structural participation in flagellated sperm motility. Reason: The conserved N-DRC scaffold performs structural work required for flagellar bending. The literal mouse donor includes sperm localization and expression evidence; the PAINT assertion at PTN002834877 is an inherited phylogenetic judgment, not a donor-count argument. Independently, PMID:37950557 reports a human biallelic DRC4 splice-site variant with immotile, structurally abnormal sperm. Splicing is predicted rather than RNA-confirmed, and C-terminal antibody loss allows absence or truncation. These limits do not turn DRC4 into a passive substrate of motility. The 2023 source supplies a human respiratory structural inventory, not a sperm preparation; sperm evidence comes from the separate clinical study. |
| GO:0031267 small GTPase binding | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: Retain the accessory small-GTPase association. Reason: The exact mouse donor Q60779 has IDA small-GTPase binding from PMID:18396146. Its authentic abstract reports Rab3B association, preferential recognition of the GTP-bound state and Golgi context in NIH3T3 cells. Full construct and purification Methods were not recovered, and no human DRC4 GEF, GAP or exocytic mechanism is inferred. This positive ortholog association is retained outside the structural core. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: UniProtKB:Q60779 SUPPORTS TRANSFER Mouse Gas8 is the literal donor. Its current experimental annotations and source publications were checked; organism and assay boundaries remain explicit in the annotation reason. ensembl:ENSMUSP00000090730 SUPPORTS TRANSFER The source snapshot maps this mouse translation to the same Q60779 donor. It is an orthology-mapping identifier, not a separate experimental assay. InterPro:IPR039308 SUPPORTS TRANSFER This domain/family mapping is a computational source. Transfer is corroborated by the independently examined mouse and human evidence described in the reason; the mapping is not presented as direct chemistry. Supporting Evidence: |
| GO:0031514 motile cilium | IDA PMID:26387594 Loss-of-Function GAS8 Mutations Cause Primary Ciliary Dyskin... | ACCEPT | Summary: Retain motile-cilium localization. Reason: Human respiratory biopsy immunofluorescence and loss-of-function observations establish the motile-ciliary pool; the literal mouse source is concordant. This names a functioning anatomical compartment, while N-DRC membership is separately represented. It does not exclude the independently observed primary-cilium and nonciliary pools. |
| GO:0031514 motile cilium | IDA PMID:26387594 Loss-of-Function GAS8 Mutations Cause Primary Ciliary Dyskin... | ACCEPT | Summary: Retain motile-cilium localization. Reason: Human respiratory biopsy immunofluorescence and loss-of-function observations establish the motile-ciliary pool; the literal mouse source is concordant. This names a functioning anatomical compartment, while N-DRC membership is separately represented. It does not exclude the independently observed primary-cilium and nonciliary pools. |
| GO:0031514 motile cilium | IEA GO_REF:0000120 | ACCEPT | Summary: Retain motile-cilium localization. Reason: Human respiratory biopsy immunofluorescence and loss-of-function observations establish the motile-ciliary pool; the literal mouse source is concordant. This names a functioning anatomical compartment, while N-DRC membership is separately represented. It does not exclude the independently observed primary-cilium and nonciliary pools. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:Q60779 SUPPORTS TRANSFER Mouse Gas8 is the literal donor. Its current experimental annotations and source publications were checked; organism and assay boundaries remain explicit in the annotation reason. ensembl:ENSMUSP00000090730 SUPPORTS TRANSFER The source snapshot maps this mouse translation to the same Q60779 donor. It is an orthology-mapping identifier, not a separate experimental assay. InterPro:IPR025593 SUPPORTS TRANSFER This domain/family mapping is a computational source. Transfer is corroborated by the independently examined mouse and human evidence described in the reason; the mapping is not presented as direct chemistry. UniProtKB-SubCell:SL-0117 SUPPORTS TRANSFER The original UniProt subcellular mapping is preserved. The named location and any narrower human refinement are evaluated separately in the annotation reason; nonexclusive pools remain distinct. |
| GO:0031514 motile cilium | ISS GO_REF:0000024 | ACCEPT | Summary: Retain motile-cilium localization. Reason: Human respiratory biopsy immunofluorescence and loss-of-function observations establish the motile-ciliary pool; the literal mouse source is concordant. This names a functioning anatomical compartment, while N-DRC membership is separately represented. It does not exclude the independently observed primary-cilium and nonciliary pools. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:Q60779 SUPPORTS TRANSFER Mouse Gas8 is the literal donor. Its current experimental annotations and source publications were checked; organism and assay boundaries remain explicit in the annotation reason. |
| GO:0035082 axoneme assembly | IBA GO_REF:0000033 | ACCEPT | Summary: Retain structural participation in axoneme assembly. Reason: DRC4 is an integral N-DRC scaffold whose loss destabilizes DRC3 localization and axonemal organization. The human and mouse sources therefore support structural participation in axoneme assembly, not a claim of catalytic assembly machinery or universal dynein-arm loss. The exact PTN000774884 IBD and target descent are verified, with human self-grounding and mouse experimental evidence. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000774884 SUPPORTS TRANSFER The literal IBD is present for this term and node in the preserved PAINT source, and the target descends from that node. Experimental descendant contexts are recorded in the portable source extract. The target among descendants is not circular evidence. |
| GO:0035082 axoneme assembly | IMP PMID:27120127 Mutations in GAS8, a Gene Encoding a Nexin-Dynein Regulatory... | ACCEPT | Summary: Retain structural participation in axoneme assembly. Reason: DRC4 is an integral N-DRC scaffold whose loss destabilizes DRC3 localization and axonemal organization. The human and mouse sources therefore support structural participation in axoneme assembly, not a claim of catalytic assembly machinery or universal dynein-arm loss. PMID:27120127 remains abstract-only, but reports human axonemal disorganization and DRC3 mislocalization; retain this curated assertion with independently recovered human evidence rather than rejecting it from incomplete access. |
| GO:0036064 ciliary basal body | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: Retain the conserved accessory basal-body pool. Reason: Mouse mIMCD3 Gas8 staining overlaps the gamma-tubulin-positive base of primary cilia in the recovered PMID:16643277 original, with an additional basal-body context in PMID:21659505. The PAINT PTN000774884 assertion includes the mouse and worm experimental branches, and human target descent is verified. This positive ortholog localization supports the accessory basal-body pool; it is not inferred from a bulk cytoplasmic fraction or asserted to be exclusive. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: PANTHER:PTN000774884 SUPPORTS TRANSFER The literal IBD is present for this term and node in the preserved PAINT source, and the target descends from that node. Experimental descendant contexts are recorded in the portable source extract. The target among descendants is not circular evidence. |
| GO:0036064 ciliary basal body | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Retain the conserved accessory basal-body pool. Reason: Mouse mIMCD3 Gas8 staining overlaps the gamma-tubulin-positive base of primary cilia in the recovered PMID:16643277 original, with an additional basal-body context in PMID:21659505. The PAINT PTN000774884 assertion includes the mouse and worm experimental branches, and human target descent is verified. This positive ortholog localization supports the accessory basal-body pool; it is not inferred from a bulk cytoplasmic fraction or asserted to be exclusive. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: UniProtKB:Q60779 SUPPORTS TRANSFER Mouse Gas8 is the literal donor. Its current experimental annotations and source publications were checked; organism and assay boundaries remain explicit in the annotation reason. ensembl:ENSMUSP00000090730 SUPPORTS TRANSFER The source snapshot maps this mouse translation to the same Q60779 donor. It is an orthology-mapping identifier, not a separate experimental assay. |
| GO:0036064 ciliary basal body | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Retain the conserved accessory basal-body pool. Reason: Mouse mIMCD3 Gas8 staining overlaps the gamma-tubulin-positive base of primary cilia in the recovered PMID:16643277 original, with an additional basal-body context in PMID:21659505. The PAINT PTN000774884 assertion includes the mouse and worm experimental branches, and human target descent is verified. This positive ortholog localization supports the accessory basal-body pool; it is not inferred from a bulk cytoplasmic fraction or asserted to be exclusive. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: UniProtKB:Q60779 SUPPORTS TRANSFER Mouse Gas8 is the literal donor. Its current experimental annotations and source publications were checked; organism and assay boundaries remain explicit in the annotation reason. |
| GO:0036126 sperm flagellum | IBA GO_REF:0000033 | ACCEPT | Summary: Retain the sperm-flagellum structural pool. Reason: The literal PAINT/mouse source supports sperm-flagellum localization, and current human HPA staining assigns supported midpiece, principal-piece and end-piece locations. The human infertility study independently detects loss of C-terminal flagellar staining in the affected patient. Retain this structural pool without treating antibody patterns as isoform-specific functional proof. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN002834877 SUPPORTS TRANSFER The literal IBD is present for this term and node in the preserved PAINT source, and the target descends from that node. Experimental descendant contexts are recorded in the portable source extract. The target among descendants is not circular evidence. Supporting Evidence: GO_REF:0000052 UniProtKB:Q60779 https://www.proteinatlas.org/ENSG00000141013-GAS8/subcellular |
| GO:0036126 sperm flagellum | IEA GO_REF:0000107 | ACCEPT | Summary: Retain the sperm-flagellum structural pool. Reason: The literal PAINT/mouse source supports sperm-flagellum localization, and current human HPA staining assigns supported midpiece, principal-piece and end-piece locations. The human infertility study independently detects loss of C-terminal flagellar staining in the affected patient. Retain this structural pool without treating antibody patterns as isoform-specific functional proof. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:Q60779 SUPPORTS TRANSFER Mouse Gas8 is the literal donor. Its current experimental annotations and source publications were checked; organism and assay boundaries remain explicit in the annotation reason. ensembl:ENSMUSP00000090730 SUPPORTS TRANSFER The source snapshot maps this mouse translation to the same Q60779 donor. It is an orthology-mapping identifier, not a separate experimental assay. Supporting Evidence: GO_REF:0000052 UniProtKB:Q60779 https://www.proteinatlas.org/ENSG00000141013-GAS8/subcellular |
| GO:0036126 sperm flagellum | ISS GO_REF:0000024 | ACCEPT | Summary: Retain the sperm-flagellum structural pool. Reason: The literal PAINT/mouse source supports sperm-flagellum localization, and current human HPA staining assigns supported midpiece, principal-piece and end-piece locations. The human infertility study independently detects loss of C-terminal flagellar staining in the affected patient. Retain this structural pool without treating antibody patterns as isoform-specific functional proof. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:Q60779 SUPPORTS TRANSFER Mouse Gas8 is the literal donor. Its current experimental annotations and source publications were checked; organism and assay boundaries remain explicit in the annotation reason. Supporting Evidence: GO_REF:0000052 UniProtKB:Q60779 https://www.proteinatlas.org/ENSG00000141013-GAS8/subcellular |
| GO:0044782 cilium organization | IC PMID:26387594 Loss-of-Function GAS8 Mutations Cause Primary Ciliary Dyskin... | MODIFY | Summary: Specify axoneme assembly within cilium organization. Reason: The original IC assertion is grounded in DRC3 localization dependence on DRC4. The established structural role is assembly and integrity of the axonemal N-DRC, and GO:0035082 is a typed descendant of cilium organization. This refinement does not claim DRC4 assembles every ciliary compartment. Propagation Review Root cause: TERM SCOPING PROBLEM Failure modes: GRANULARITY MISMATCH Sources checked: GO:0061512 SUPPORTS TRANSFER The curator inference uses the preserved protein-localization assertion. Human DRC3 loss from axonemes and the structural evidence support the stated assembly context. Proposed replacements: axoneme assembly |
| GO:0045880 positive regulation of smoothened signaling pathway | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: Retain the context-dependent positive Smoothened-regulatory role. Reason: The actual PAINT node and mouse/zebrafish source branches are verified. PMID:21659505 reports human GAS8–SMO two-hybrid fragments, mouse cellular signaling and trafficking perturbations, and zebrafish morpholino/rescue evidence. Mouse full-length Gas8 enhancement was variable and nonsignificant, whereas the engineered GMAD–CT construct had significant GRK2-dependent effects. The later mouse gene-trap study found normal SAG-dependent Smo accumulation in MEFs and normal tested patterning, while retaining an N-terminal fusion. Preserve both context-specific findings without assuming compensation, a universal signaling role, or universal absence. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: PANTHER:PTN000774884 SUPPORTS TRANSFER The literal IBD is present for this term and node in the preserved PAINT source, and the target descends from that node. Experimental descendant contexts are recorded in the portable source extract. The target among descendants is not circular evidence. |
| GO:0045880 positive regulation of smoothened signaling pathway | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Retain the context-dependent positive Smoothened-regulatory role. Reason: The actual PAINT node and mouse/zebrafish source branches are verified. PMID:21659505 reports human GAS8–SMO two-hybrid fragments, mouse cellular signaling and trafficking perturbations, and zebrafish morpholino/rescue evidence. Mouse full-length Gas8 enhancement was variable and nonsignificant, whereas the engineered GMAD–CT construct had significant GRK2-dependent effects. The later mouse gene-trap study found normal SAG-dependent Smo accumulation in MEFs and normal tested patterning, while retaining an N-terminal fusion. Preserve both context-specific findings without assuming compensation, a universal signaling role, or universal absence. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: UniProtKB:Q60779 SUPPORTS TRANSFER Mouse Gas8 is the literal donor. Its current experimental annotations and source publications were checked; organism and assay boundaries remain explicit in the annotation reason. ensembl:ENSMUSP00000090730 SUPPORTS TRANSFER The source snapshot maps this mouse translation to the same Q60779 donor. It is an orthology-mapping identifier, not a separate experimental assay. |
| GO:0045880 positive regulation of smoothened signaling pathway | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Retain the context-dependent positive Smoothened-regulatory role. Reason: The actual PAINT node and mouse/zebrafish source branches are verified. PMID:21659505 reports human GAS8–SMO two-hybrid fragments, mouse cellular signaling and trafficking perturbations, and zebrafish morpholino/rescue evidence. Mouse full-length Gas8 enhancement was variable and nonsignificant, whereas the engineered GMAD–CT construct had significant GRK2-dependent effects. The later mouse gene-trap study found normal SAG-dependent Smo accumulation in MEFs and normal tested patterning, while retaining an N-terminal fusion. Preserve both context-specific findings without assuming compensation, a universal signaling role, or universal absence. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: UniProtKB:Q60779 SUPPORTS TRANSFER Mouse Gas8 is the literal donor. Its current experimental annotations and source publications were checked; organism and assay boundaries remain explicit in the annotation reason. |
| GO:0048870 cell motility | IEA GO_REF:0000002 | MODIFY | Summary: Specify the established flagellated-sperm motility context. Reason: Independent human sperm evidence and the N-DRC structural role support the specific flagellated-sperm motility process, a typed descendant of both cell motility and cilium movement involved in cell motility. The imported source identity is preserved. Propagation Review Root cause: TERM SCOPING PROBLEM Failure modes: GRANULARITY MISMATCH Sources checked: InterPro:IPR025593 SUPPORTS TRANSFER This domain/family mapping is a computational source. Transfer is corroborated by the independently examined mouse and human evidence described in the reason; the mapping is not presented as direct chemistry. InterPro:IPR039308 SUPPORTS TRANSFER This domain/family mapping is a computational source. Transfer is corroborated by the independently examined mouse and human evidence described in the reason; the mapping is not presented as direct chemistry. Proposed replacements: flagellated sperm motility |
| GO:0060294 cilium movement involved in cell motility | IMP PMID:27472056 Mutation of Growth Arrest Specific 8 Reveals a Role in Motil... | MODIFY | Summary: Retain the measured epithelial ciliary-movement process. Reason: PMID:27472056 measures ciliary waveform, beating and fluid flow in mouse tracheal and ependymal contexts using an intron-7 gene-trap model that retains an N-terminal fusion. GO:0060294 requires ciliary movement that displaces a cell; movement of fluid by stationary epithelium does not establish that additional claim. GO:0003341 cilium movement preserves the positive source endpoint. Independent human sperm evidence remains in its own assertions and is not substituted for this mouse epithelial experiment. Proposed replacements: cilium movement Supporting Evidence: |
| GO:0061512 protein localization to cilium | IMP PMID:26387594 Loss-of-Function GAS8 Mutations Cause Primary Ciliary Dyskin... | ACCEPT | Summary: DRC4 supports ciliary localization of another N-DRC component. Reason: The human loss-of-function study shows LRRC48/DRC3 missing from DRC4-deficient axonemes while several dynein-arm markers remain. As an integral scaffold DRC4 supplies structural work for localization/retention of the associated component. This is not an assertion that DRC4 transports all ciliary cargo or that every protein is lost. Supporting Evidence: |
| GO:0065003 protein-containing complex assembly | NAS PMID:26387594 Loss-of-Function GAS8 Mutations Cause Primary Ciliary Dyskin... | ACCEPT | Summary: Retain the N-DRC complex-assembly role. Reason: PMID:26387594 shows loss of axonemal DRC3/LRRC48 in human GAS8-deficient respiratory cilia and explicitly infers defective N-DRC assembly from that observation. The existing NAS assertion is retained with this distinction between observed partner localization/retention and inferred complex assembly. Conserved DRC1/DRC2/DRC4 scaffold architecture and homologous human DRC4 placement in PMID:37258679 support structural participation. There is no purified human reassembly assay or claim that all motor dynein arms disappear. Complex assembly and axoneme assembly are distinct processes in the captured typed ontology closure. |
| GO:0097225 sperm midpiece | IDA GO_REF:0000052 | ACCEPT | Summary: Retain supported human sperm midpiece localization. Reason: The current HPA041311 sperm ICC/IF record explicitly assigns supported midpiece localization. This is compatible with DRC4’s flagellar scaffold role and human sperm structural pathology. It does not establish differential function of the two natural splice products or require every axonemal subcomplex to be resolved in the immunofluorescence image. Supporting Evidence: GO_REF:0000052 https://www.proteinatlas.org/ENSG00000141013-GAS8/subcellular |
| GO:0097228 sperm principal piece | IDA GO_REF:0000052 | ACCEPT | Summary: Retain supported human sperm principal piece localization. Reason: The current HPA041311 sperm ICC/IF record explicitly assigns supported principal piece localization. This is compatible with DRC4’s flagellar scaffold role and human sperm structural pathology. It does not establish differential function of the two natural splice products or require every axonemal subcomplex to be resolved in the immunofluorescence image. Supporting Evidence: GO_REF:0000052 https://www.proteinatlas.org/ENSG00000141013-GAS8/subcellular |
| GO:0097229 sperm end piece | IDA GO_REF:0000052 | ACCEPT | Summary: Retain supported human sperm end piece localization. Reason: The current HPA041311 sperm ICC/IF record explicitly assigns supported end piece localization. This is compatible with DRC4’s flagellar scaffold role and human sperm structural pathology. It does not establish differential function of the two natural splice products or require every axonemal subcomplex to be resolved in the immunofluorescence image. Supporting Evidence: GO_REF:0000052 https://www.proteinatlas.org/ENSG00000141013-GAS8/subcellular |
| GO:0097729 9+2 motile cilium | IEA GO_REF:0000107 | ACCEPT | Summary: Retain the 9+2 motile-cilium pool. Reason: The mouse-derived annotation is concordant with human respiratory and sperm axonemal evidence. DRC4 functions as an N-DRC structural subunit in these 9+2 motile organelles; this does not exclude nonmotile or cytoplasmic contexts. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:Q60779 SUPPORTS TRANSFER Mouse Gas8 is the literal donor. Its current experimental annotations and source publications were checked; organism and assay boundaries remain explicit in the annotation reason. ensembl:ENSMUSP00000090730 SUPPORTS TRANSFER The source snapshot maps this mouse translation to the same Q60779 donor. It is an orthology-mapping identifier, not a separate experimental assay. |
| GO:1903566 positive regulation of protein localization to cilium | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Retain bounded regulation of ciliary protein localization. Reason: The mouse Smoothened experiments support a positive effect on receptor ciliary accumulation in a defined signaling context, while human N-DRC loss affects DRC3 localization. These are positive but mechanistically distinct observations. Retain this regulatory process as accessory, with structural assembly/localization represented separately in the core; do not infer a general cargo-transport reaction. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: UniProtKB:Q60779 SUPPORTS TRANSFER Mouse Gas8 is the literal donor. Its current experimental annotations and source publications were checked; organism and assay boundaries remain explicit in the annotation reason. ensembl:ENSMUSP00000090730 SUPPORTS TRANSFER The source snapshot maps this mouse translation to the same Q60779 donor. It is an orthology-mapping identifier, not a separate experimental assay. |
| GO:1903566 positive regulation of protein localization to cilium | IMP PMID:27120127 Mutations in GAS8, a Gene Encoding a Nexin-Dynein Regulatory... | KEEP AS NON CORE | Summary: Retain bounded regulation of ciliary protein localization. Reason: The mouse Smoothened experiments support a positive effect on receptor ciliary accumulation in a defined signaling context, while human N-DRC loss affects DRC3 localization. These are positive but mechanistically distinct observations. Retain this regulatory process as accessory, with structural assembly/localization represented separately in the core; do not infer a general cargo-transport reaction. The PMID:27120127 abstract supports DRC3 mislocalization, but its complete Methods remain unavailable, so no unobserved trafficking assay is asserted. |
| GO:0140378 protein complex scaffold activity | NAS PMID:37258679 Axonemal structures reveal mechanoregulatory and disease mec... | NEW | Summary: Propose the structural N-DRC scaffold activity. Reason: DRC4 supplies part of the coiled-coil framework holding the N-DRC together. PMID:37258679 resolves detailed DRC1/DRC2/DRC4 geometry in algae and places homologous human DRC4 models in respiratory-axoneme density, with O95995 in the human structural inventory. Human GAS8 deficiency in PMID:26387594 removes axonemal DRC3, corroborating structural integrity and partner retention. This is structural work by DRC4, not merely necessity for another protein’s catalytic reaction. The human model and conserved geometry are not a purified human reassembly assay. No existing source or replacement term is an is_a/part_of ancestor or descendant of this specific MF. |
| GO:0003352 regulation of cilium movement | IMP PMID:26387594 Loss-of-Function GAS8 Mutations Cause Primary Ciliary Dyskin... | NEW | Summary: Propose regulation of ciliary movement by the N-DRC scaffold. Reason: Human GAS8-deficient respiratory cilia in PMID:26387594 have reduced bending amplitude despite reported normal 5–8 Hz frequency. Mouse experiments in PMID:27472056 independently show altered waveform, beating and fluid flow. Structural evidence in PMID:37258679 places DRC4 in the N-DRC framework linking and constraining neighboring doublets: DRC4 contributes the structure coordinating movement rather than acting only as a required substrate. Detailed geometry is algal and homologous human placement is model-based; motor ATPase activity is not assigned. The same-role comparator audit includes human DRC2/Q8IXS2, which carries this term by IBA and IMP, and algal DRC1/P0DL09, which has other assembly annotations. This is not an absence-only gap claim. The term has no existing is_a/part_of ancestor or descendant among this gene’s source/replacement assertions; its regulates relation to cilium movement is distinct. |
Loading supporting content…
Download this section (compressed HTML)Q: Which native human cell contexts require DRC4 for Smoothened regulation, and how do they differ from the mouse gene-trap MEF and developmental assays?
Q: Does the natural 1–25 deletion alter N-DRC incorporation or accessory localization, without assuming equivalence from antibody-recognized bands?
Experiment: Compare sequence-verified O95995-1 and O95995-2 at matched near-endogenous expression in DRC4-deficient human ciliated cells. Measure N-DRC incorporation, DRC3 localization, beat amplitude and frequency separately, then assess Smoothened accumulation and signaling under defined stimulation. Include native-expression controls and quantify residual mutant fragments rather than assuming a complete null.
Hypothesis: The two natural DRC4 products differ in their incorporation or accessory localization without necessarily differing in the core scaffold role.
Type: Isoform-resolved complementation and localization
Loading supporting content…
Download this section (compressed HTML)Loading supporting content…
Download this section (compressed HTML)Loading supporting content…
Download this section (compressed HTML)