DYNC1H1

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0001673 male germ cell nucleus	NAS PMID:36973253 Distinct dynein complexes defined by DYNLRB1 and DYNLRB2 reg...	MARK AS OVER ANNOTATED	Summary: Male germ cell nucleus (NAS) from a mouse DYNLRB2 meiosis paper; the paper places DYNC1H1 at spindle poles of spermatocytes, not in the nucleus. Reason: Cytoplasmic dynein 1 is a cytoplasmic motor. In spermatocytes it acts at spindle poles and on the cytoplasmic face of the nuclear envelope; no intranuclear pool is described. The NAS statement does not support a nuclear location as a meaningful annotation. Supporting Evidence: PMID:36973253 the localization of the dynein–dynactin subunits DYNC1H1, DYNLL1, and p150 at spindle poles were not affected in Dynlrb2−⁄− spermatocytes file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasm, cytoskeleton
GO:0003723 RNA binding	HDA PMID:22658674 Insights into RNA biology from an atlas of mammalian mRNA-bi...	MARK AS OVER ANNOTATED	Summary: RNA binding from high-throughput mRNA interactome capture. Reason: DYNC1H1 was recovered in a UV-crosslinking mRNA-interactome screen. The heavy chain has no known RNA-binding domain; RNA transport by dynein occurs through adaptors (e.g. BICD2/Egalitarian-like complexes) rather than direct RNA binding by the motor. The HDA hit is not evidence of an RNA-binding function. Supporting Evidence: PMID:22658674 Employing two complementary protocols for covalent UV crosslinking of RBPs to RNA
GO:0003723 RNA binding	HDA PMID:22681889 The mRNA-bound proteome and its global occupancy profile on ...	MARK AS OVER ANNOTATED	Summary: RNA binding from high-throughput mRNA interactome capture. Reason: DYNC1H1 was recovered in a UV-crosslinking mRNA-interactome screen. The heavy chain has no known RNA-binding domain; RNA transport by dynein occurs through adaptors (e.g. BICD2/Egalitarian-like complexes) rather than direct RNA binding by the motor. The HDA hit is not evidence of an RNA-binding function. Supporting Evidence: PMID:22681889 We developed a photoreactive nucleotide-enhanced UV crosslinking and oligo(dT) purification approach to identify the mRNA-bound proteome
GO:0005515 protein binding	IPI PMID:15161933 Comprehensive proteomic analysis of interphase and mitotic 1...	REMOVE	Summary: Protein binding (IPI). 14-3-3 zeta (YWHAZ) affinity proteomics hit from HeLa extracts (abstract-only cache). Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:15161933 14-3-3-binding proteins were purified from extracts of interphase and mitotic HeLa cells using specific peptide elution from 14-3-3 zeta affinity columns.
GO:0005515 protein binding	IPI PMID:17043677 Disrupted in Schizophrenia 1 Interactome: evidence for the c...	REMOVE	Summary: Protein binding (IPI). DISC1 yeast two-hybrid interactome hit. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:17043677 we have generated a network of protein-protein interactions (PPIs) around DISC1.
GO:0005515 protein binding	IPI PMID:17500595 Huntingtin interacting proteins are genetic modifiers of neu...	REMOVE	Summary: Protein binding (IPI). Huntingtin (HTT) interactor screen hit. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms.
GO:0005515 protein binding	IPI PMID:17548833 Huntingtin facilitates dynein/dynactin-mediated vesicle tran...	REMOVE	Summary: Protein binding (IPI). Huntingtin-dynein association; the directly mapped HTT contact is on the dynein intermediate chain, not the heavy chain. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:17548833 Htt binds to dynein and acts in a complex along with dynactin and Htt-associated protein-1 to facilitate vesicular transport.
GO:0005515 protein binding	IPI PMID:21163940 Interactome mapping suggests new mechanistic details underly...	REMOVE	Summary: Protein binding (IPI). PSEN2 yeast two-hybrid hit from an Alzheimer disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:21163940 we report 200 high-confidence protein-protein interactions between eight confirmed AD-related genes and 66 candidates.
GO:0005515 protein binding	IPI PMID:31092558 Rab46 integrates Ca(2+) and histamine signaling to regulate ...	REMOVE	Summary: Protein binding (IPI). Interaction with the Rab46 (CRACR2A isoform 2) cargo adaptor that drives dynein-dependent Weibel-Palade body transport. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:31092558 GTP-bound Rab46 evokes dynein-dependent retrograde transport of a subset of WPBs along microtubules. file:human/DYNC1H1/DYNC1H1-uniprot.txt Interacts with isoform 2 of CRACR2A
GO:0005515 protein binding	IPI PMID:31413325 HENA, heterogeneous network-based data set for Alzheimer's d...	REMOVE	Summary: Protein binding (IPI). DISC1 interaction compiled in the HENA Alzheimer disease network dataset. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms.
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	REMOVE	Summary: Protein binding (IPI). One of many systematic yeast two-hybrid hits from a neurodegenerative-disease interactome screen. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins
GO:0005515 protein binding	IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce...	REMOVE	Summary: Protein binding (IPI). OpenCell endogenous-tag interactome hit (YWHAZ). Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms.
GO:0005515 protein binding	IPI PMID:36692009 Structures of human dynein in complex with the lissencephaly...	REMOVE	Summary: Protein binding (IPI). Structural characterization of the human dynein-LIS1 complex; the LIS1 regulatory interaction is real but there is no specific MF term for binding a dynein regulator on the heavy chain. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:36692009 The lissencephaly 1 protein, LIS1, is mutated in type-1 lissencephaly and is a key regulator of cytoplasmic dynein-1.
GO:0005515 protein binding	IPI PMID:36950384 Protein interaction studies in human induced neurons indicat...	REMOVE	Summary: Protein binding (IPI). Interactions from induced-neuron IP-MS of autism-associated proteins (ANK2, GIGYF1). Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:36950384 including multiple interactions involving SMARCC2, ELAVL3, DPYSL2, and DYNC1H1.
GO:0005515 protein binding	IPI PMID:36950384 Protein interaction studies in human induced neurons indicat...	REMOVE	Summary: Protein binding (IPI). Interactions from induced-neuron IP-MS of autism-associated proteins (ANK2, GIGYF1). Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:36950384 including multiple interactions involving SMARCC2, ELAVL3, DPYSL2, and DYNC1H1.
GO:0005515 protein binding	IPI PMID:38547289 Molecular mechanism of dynein-dynactin complex assembly by L...	REMOVE	Summary: Protein binding (IPI). Cryo-EM of dynein-dynactin-JIP3 with LIS1; LIS1 contacts on dynein are structurally defined. Reason: GO:0005515 protein binding is uninformative about DYNC1H1 function. The reported interaction may well be real, but it does not identify a molecular activity of the heavy chain; the functionally meaningful dynein interactions (intermediate chain, light intermediate chain, dynactin/adaptor assembly) are captured by more specific terms. Supporting Evidence: PMID:38547289 Unexpectedly, LIS1 binds dynactin's p150 subunit, tethering it along the length of dynein.
GO:0005524 ATP binding	IEA GO_REF:0000002	ACCEPT	Summary: ATP binding from InterPro; DYNC1H1 has P-loop ATP-binding sites in its AAA+ ring and hydrolyses ATP to power movement. Reason: The motor domain contains six AAA+ modules with ATP-binding motifs, the primary hydrolytic site being AAA1. ATP binding is intrinsic to the motor mechanism. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP.
GO:0005576 extracellular region	TAS Reactome:R-HSA-6798751	REMOVE	Summary: Extracellular/azurophil granule lumen location from the Reactome neutrophil degranulation proteomic event. Reason: Cytoplasmic dynein 1 heavy chain is a cytosolic, microtubule-associated motor with no signal peptide and no known secretion route. Its inclusion in the azurophil granule proteome reflects proteomic co-purification and is not a biologically meaningful location. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasm, cytoskeleton
GO:0005737 cytoplasm	IEA GO_REF:0000107	ACCEPT	Summary: Cytoplasm (IEA, Ensembl Compara). Reason: DYNC1H1 is a cytoplasmic motor. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasm, cytoskeleton file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005813 centrosome	IDA PMID:21399614 Novel asymmetrically localizing components of human centroso...	ACCEPT	Summary: Centrosome localization. Reason: Dynein accumulates at centrosomes/microtubule-organizing centers as the minus-end destination of its transport and during spindle assembly; supported by centrosome proteomics and GFP-DYNC1H1 imaging. Supporting Evidence: PMID:21399614 we distinguished 126 known and 40 candidate centrosomal proteins
GO:0005813 centrosome	IDA PMID:28602352 Cryo-EM Reveals How Human Cytoplasmic Dynein Is Auto-inhibit...	ACCEPT	Summary: Centrosome localization. Reason: Dynein accumulates at centrosomes/microtubule-organizing centers as the minus-end destination of its transport and during spindle assembly; supported by centrosome proteomics and GFP-DYNC1H1 imaging. Supporting Evidence: PMID:28602352 GFP-mtDyn accumulates more at the centrosomes than GFP-wtDyn.
GO:0005829 cytosol	TAS Reactome:R-HSA-141409	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-141422	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-141431	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-141439	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-1638803	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-1638821	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-2213248	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-2467809	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-2467811	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-2468287	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-2484822	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-2574840	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-2574845	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-3000310	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-3000319	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-375302	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-380272	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-380283	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-380294	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-380303	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-380311	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-380316	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-380455	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-380508	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-5617816	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-5626220	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-5626223	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-5626227	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-5626228	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-5626681	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-5626699	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-5638009	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-5666129	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-5666160	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-5666169	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-6809003	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-6809006	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-8849350	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-8849353	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-8853405	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-8853419	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-9614343	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-9614367	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-9646348	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-9646679	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-9646685	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-HSA-9648114	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005829 cytosol	TAS Reactome:R-NUL-9646387	ACCEPT	Summary: Cytosol (TAS, Reactome). Reason: The soluble pool of cytoplasmic dynein 1 is cytosolic; Reactome places dynein-dynactin in the cytosol for its transport, kinetochore, centrosome and Golgi-traffic events. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005856 cytoskeleton	IEA GO_REF:0000044	ACCEPT	Summary: Cytoskeleton (IEA from UniProt subcellular location). Reason: Dynein binds and moves along microtubules. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasm, cytoskeleton
GO:0005868 cytoplasmic dynein complex	IBA GO_REF:0000033	ACCEPT	Summary: Part of cytoplasmic dynein complex. Reason: DYNC1H1 is the catalytic heavy chain of cytoplasmic dynein 1; its homodimer is the scaffold on which intermediate, light intermediate and light chains assemble. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt The ICs and LICs bind directly to the HC dimer and dynein LCs assemble on the IC dimer
GO:0005868 cytoplasmic dynein complex	IDA PMID:21723285 Recombinant human cytoplasmic dynein heavy chain 1 and 2: ob...	ACCEPT	Summary: Part of cytoplasmic dynein complex. Reason: DYNC1H1 is the catalytic heavy chain of cytoplasmic dynein 1; its homodimer is the scaffold on which intermediate, light intermediate and light chains assemble. Supporting Evidence: PMID:21723285 In an in vitro MT gliding assay, both dynein-1 and dynein-2 showed minus-end-directed motor activities. file:human/DYNC1H1/DYNC1H1-uniprot.txt The ICs and LICs bind directly to the HC dimer and dynein LCs assemble on the IC dimer
GO:0005868 cytoplasmic dynein complex	IDA PMID:25272277 A Ras-like domain in the light intermediate chain bridges th...	ACCEPT	Summary: Part of cytoplasmic dynein complex. Reason: DYNC1H1 is the catalytic heavy chain of cytoplasmic dynein 1; its homodimer is the scaffold on which intermediate, light intermediate and light chains assemble. Supporting Evidence: PMID:25272277 We show that the LIC G domain binds the dynein heavy chain using a conserved patch of aromatic residues file:human/DYNC1H1/DYNC1H1-uniprot.txt The ICs and LICs bind directly to the HC dimer and dynein LCs assemble on the IC dimer
GO:0005868 cytoplasmic dynein complex	IEA GO_REF:0000117	ACCEPT	Summary: Part of cytoplasmic dynein complex. Reason: DYNC1H1 is the catalytic heavy chain of cytoplasmic dynein 1; its homodimer is the scaffold on which intermediate, light intermediate and light chains assemble. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt The ICs and LICs bind directly to the HC dimer and dynein LCs assemble on the IC dimer
GO:0005868 cytoplasmic dynein complex	IPI PMID:24986880 In vitro reconstitution of a highly processive recombinant h...	ACCEPT	Summary: Part of cytoplasmic dynein complex. Reason: DYNC1H1 is the catalytic heavy chain of cytoplasmic dynein 1; its homodimer is the scaffold on which intermediate, light intermediate and light chains assemble. Supporting Evidence: PMID:24986880 Cytoplasmic dynein is an approximately 1.4 MDa multi-protein complex that transports many cellular cargoes towards the minus ends of microtubules. file:human/DYNC1H1/DYNC1H1-uniprot.txt The ICs and LICs bind directly to the HC dimer and dynein LCs assemble on the IC dimer
GO:0005868 cytoplasmic dynein complex	NAS PMID:8666668 Mammalian cells express three distinct dynein heavy chains t...	ACCEPT	Summary: Part of cytoplasmic dynein complex. Reason: DYNC1H1 is the catalytic heavy chain of cytoplasmic dynein 1; its homodimer is the scaffold on which intermediate, light intermediate and light chains assemble. Supporting Evidence: PMID:8666668 We describe two dynein heavy chain (DHC)-like polypeptides (DHCs 2 and 3) that are distinct from the heavy chain of conventional cytoplasmic dynein (DHC1) file:human/DYNC1H1/DYNC1H1-uniprot.txt The ICs and LICs bind directly to the HC dimer and dynein LCs assemble on the IC dimer
GO:0005874 microtubule	IDA PMID:21525035 PEX14 is required for microtubule-based peroxisome motility ...	ACCEPT	Summary: Microtubule (IDA) from a PEX14 peroxisome-motility study (abstract-only cache). Reason: Dynein heavy chain binds microtubules through its stalk microtubule-binding domain, so association with microtubules is expected. The cached abstract does not describe the DYNC1H1 localization experiment, but the location is well established and the curator had the full text. Supporting Evidence: PMID:21525035 peroxisomal remnants in PEX14-deficient cells have lost their ability to move along microtubules. file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005881 cytoplasmic microtubule	IBA GO_REF:0000033	ACCEPT	Summary: Cytoplasmic microtubule (IBA). Reason: Cytoplasmic dynein 1 walks on cytoplasmic microtubules; the PAINT placement is consistent with the conserved motor role. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0005938 cell cortex	IBA GO_REF:0000033	ACCEPT	Summary: Cell cortex (IBA). Reason: A cortical pool of dynein anchored by NuMA-LGN-Galpha pulls on astral microtubules to position the spindle in human cells and C. elegans; cortical dynein is conserved in yeast nuclear/spindle positioning. Supporting Evidence: PMID:23027904 we demonstrated that the mere presence of dynein at that location is sufficient to direct spindle positioning in HeLa cells.
GO:0007018 microtubule-based movement	IEA GO_REF:0000002	ACCEPT	Summary: Microtubule-based movement (IEA, InterPro). Reason: Generic but correct description of the motor function; DYNC1H1 generates ATP-driven minus-end movement along microtubules. Supporting Evidence: PMID:21723285 In an in vitro MT gliding assay, both dynein-1 and dynein-2 showed minus-end-directed motor activities. file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0007052 mitotic spindle organization	IBA GO_REF:0000033	ACCEPT	Summary: Mitotic spindle organization. Reason: Dynein focuses spindle poles, separates centrosomes and contributes to bipolar spindle assembly; antibody inhibition of the heavy chain produces monopolar spindles. Supporting Evidence: PMID:8227145 Cells become arrested with unseparated centrosomes and form monopolar spindles. file:human/DYNC1H1/DYNC1H1-uniprot.txt Plays a role in mitotic spindle assembly and metaphase plate congression
GO:0007052 mitotic spindle organization	NAS PMID:8227145 Cytoplasmic dynein plays a role in mammalian mitotic spindle...	ACCEPT	Summary: Mitotic spindle organization. Reason: Dynein focuses spindle poles, separates centrosomes and contributes to bipolar spindle assembly; antibody inhibition of the heavy chain produces monopolar spindles. Supporting Evidence: PMID:8227145 Their injection into mitotic mammalian cells blocks the formation of spindles in prophase or during recovery from nocodazole treatment at later stages of mitosis. PMID:8227145 Cells become arrested with unseparated centrosomes and form monopolar spindles.
GO:0007097 nuclear migration	IBA GO_REF:0000033	ACCEPT	Summary: Nuclear migration (IBA). Reason: Dynein-driven nuclear movement is conserved from fungi (NudA/Dyn1-dependent nuclear migration) to vertebrate neuronal nucleokinesis and interkinetic nuclear migration, where dynein pulls the nucleus toward the centrosome. The IBA is well placed; the motor directly generates the force. Supporting Evidence: PMID:15173193 Dynein inhibition resulted in similar defects in both nucleus-centrosome (N-C) coupling and neuronal migration. PMID:17618279 The nucleus is transported along the trailing microtubules by dynein assisted by myosin II. file:human/DYNC1H1/DYNC1H1-deep-research-falcon.md severe retinal-layer disorganization by postnatal day 6, defective nuclear positioning and membrane-protein trafficking
GO:0008090 retrograde axonal transport	IBA GO_REF:0000033	ACCEPT	Summary: Retrograde axonal transport (IBA). Reason: Cytoplasmic dynein 1 is the principal retrograde motor in axons; heavy-chain mutations in mouse (Loa, Cra1) and human (CMT2O, SMALED1) impair retrograde transport and cause motor/sensory neuron disease. Supporting Evidence: PMID:21820100 Cytoplasmic dynein is a large multisubunit motor protein complex and has a key role in retrograde axonal transport in neurons. PMID:12730604 missense point mutations in the cytoplasmic dynein heavy chain result in progressive motor neuron degeneration in heterozygous mice file:human/DYNC1H1/DYNC1H1-deep-research-falcon.md In polarized neurons, it powers long-range retrograde axonal transport from distal processes toward the soma.
GO:0008569 minus-end-directed microtubule motor activity	IDA PMID:21723285 Recombinant human cytoplasmic dynein heavy chain 1 and 2: ob...	ACCEPT	Summary: Minus-end-directed microtubule motor activity. Reason: This is the core molecular function. Recombinant human DYNC1H1-based dynein-1 shows minus-end-directed gliding in vitro; the heavy chain carries the AAA+ ring, linker, and stalk microtubule-binding domain that constitute the motor. The contributes_to qualifier on the IDA row reflects that the functional motor is a heavy-chain dimer in complex with accessory chains and, for processive movement, dynactin plus an activating adaptor. Supporting Evidence: PMID:21723285 In an in vitro MT gliding assay, both dynein-1 and dynein-2 showed minus-end-directed motor activities. PMID:24986880 addition of dynactin together with the N-terminal region of the cargo adaptor BICD2 (BICD2N) gives rise to unidirectional dynein movement over remarkably long distances. file:human/DYNC1H1/DYNC1H1-uniprot.txt Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP.
GO:0008569 minus-end-directed microtubule motor activity	IEA GO_REF:0000002	ACCEPT	Summary: Minus-end-directed microtubule motor activity. Reason: This is the core molecular function. Recombinant human DYNC1H1-based dynein-1 shows minus-end-directed gliding in vitro; the heavy chain carries the AAA+ ring, linker, and stalk microtubule-binding domain that constitute the motor. The contributes_to qualifier on the IDA row reflects that the functional motor is a heavy-chain dimer in complex with accessory chains and, for processive movement, dynactin plus an activating adaptor. Supporting Evidence: PMID:21723285 In an in vitro MT gliding assay, both dynein-1 and dynein-2 showed minus-end-directed motor activities. PMID:24986880 addition of dynactin together with the N-terminal region of the cargo adaptor BICD2 (BICD2N) gives rise to unidirectional dynein movement over remarkably long distances. file:human/DYNC1H1/DYNC1H1-uniprot.txt Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP.
GO:0016020 membrane	HDA PMID:19946888 Defining the membrane proteome of NK cells.	KEEP AS NON CORE	Summary: Membrane (HDA) from NK-cell membrane proteomics. Reason: Dynein associates peripherally with vesicle and organelle membranes via cargo adaptors (e.g. Rab effectors bound to the light intermediate chain), so recovery in membrane fractions is plausible, but it is not an integral membrane protein and this is a low-specificity location. Supporting Evidence: PMID:19946888 The remaining species were largely involved in cellular processes and molecular functions that could be predicted to be transiently associated with membranes. PMID:25272277 We show that the LIC G domain binds the dynein heavy chain using a conserved patch of aromatic residues
GO:0030175 filopodium	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: Filopodium (IEA, projected from mouse). Reason: Dynein is found throughout the cytoplasm including neuronal growth cones and protrusions; filopodial localization is peripheral to its function. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasm, cytoskeleton
GO:0030286 dynein complex	IEA GO_REF:0000002	ACCEPT	Summary: Dynein complex (IEA). Reason: Correct but less specific than cytoplasmic dynein complex. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt The ICs and LICs bind directly to the HC dimer and dynein LCs assemble on the IC dimer
GO:0031122 cytoplasmic microtubule organization	IBA GO_REF:0000033	ACCEPT	Summary: Cytoplasmic microtubule organization (IBA). Reason: Dynein organizes cytoplasmic microtubule arrays by transporting and focusing microtubule minus ends and by cortical pulling; this is a direct motor-dependent activity. Supporting Evidence: PMID:33957056 Cytoplasmic dynein is responsible for transporting intracellular cargos in interphase cells and mediating spindle assembly and chromosome positioning during cell division. PMID:8227145 Cells become arrested with unseparated centrosomes and form monopolar spindles.
GO:0032388 positive regulation of intracellular transport	IMP PMID:27462074 Nuclear Mitotic Apparatus (NuMA) Interacts with and Regulate...	MODIFY	Summary: Positive regulation of intracellular transport (IMP): dynein is required for transport of Astrin to spindle poles. Reason: Dynein here is the transporting motor itself, not a regulator of transport. The observation (dynein-dependent accumulation of Astrin at spindle poles) is better captured as protein transport along microtubule. Proposed replacements: protein transport along microtubule Supporting Evidence: PMID:27462074 we found that cytoplasmic dynein is required for the spindle pole accumulation of Astrin, and dynein-mediated transport is important for balanced distribution of Astrin between spindle poles and kinetochores.
GO:0032388 positive regulation of intracellular transport	NAS PMID:33957056 Structure and Mechanics of Dynein Motors.	MODIFY	Summary: Positive regulation of intracellular transport (NAS) from a dynein structure/mechanics review. Reason: The review describes dynein as the motor that transports cargos, i.e. a direct executor of microtubule-based transport rather than a regulator of it. Proposed replacements: microtubule-based transport Supporting Evidence: PMID:33957056 Cytoplasmic dynein is responsible for transporting intracellular cargos in interphase cells and mediating spindle assembly and chromosome positioning during cell division.
GO:0033962 P-body assembly	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: P-body assembly (projected from mouse Dync1h1). Reason: Dynein-mediated transport has been implicated in the coalescence of RNA granules in mammalian cells; the motor contributes transport work to granule assembly, but this is a peripheral, context-dependent role. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0033962 P-body assembly	ISS GO_REF:0000024	KEEP AS NON CORE	Summary: P-body assembly (projected from mouse Dync1h1). Reason: Dynein-mediated transport has been implicated in the coalescence of RNA granules in mammalian cells; the motor contributes transport work to granule assembly, but this is a peripheral, context-dependent role. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0034063 cytoplasmic stress granule assembly	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: Cytoplasmic stress granule assembly (projected from mouse Dync1h1). Reason: Microtubule- and dynein-dependent transport promotes stress granule coalescence in mammalian cells; this is a peripheral consequence of the general transport function. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0034063 cytoplasmic stress granule assembly	ISS GO_REF:0000024	KEEP AS NON CORE	Summary: Cytoplasmic stress granule assembly (projected from mouse Dync1h1). Reason: Microtubule- and dynein-dependent transport promotes stress granule coalescence in mammalian cells; this is a peripheral consequence of the general transport function. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
GO:0035578 azurophil granule lumen	TAS Reactome:R-HSA-6798751	REMOVE	Summary: Extracellular/azurophil granule lumen location from the Reactome neutrophil degranulation proteomic event. Reason: Cytoplasmic dynein 1 heavy chain is a cytosolic, microtubule-associated motor with no signal peptide and no known secretion route. Its inclusion in the azurophil granule proteome reflects proteomic co-purification and is not a biologically meaningful location. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasm, cytoskeleton
GO:0042802 identical protein binding	IDA PMID:28602352 Cryo-EM Reveals How Human Cytoplasmic Dynein Is Auto-inhibit...	ACCEPT	Summary: Identical protein binding (IDA): the heavy chain homodimerizes through its N-terminal dimerization domain. Reason: Heavy-chain homodimerization via the NDD is structurally defined in cryo-EM and is essential for the two-headed motor; the term is informative here (self-association). Supporting Evidence: PMID:28602352 The heavy chain (HC) N-terminal dimerization domain (NDD) holds HC A and HC B together. file:human/DYNC1H1/DYNC1H1-uniprot.txt Homodimer.
GO:0045505 dynein intermediate chain binding	IBA GO_REF:0000033	ACCEPT	Summary: Dynein intermediate chain binding. Reason: The heavy chain tail binds the intermediate chains directly (UniProt maps DYNC1I2 interaction to residues 448-703). Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt The ICs and LICs bind directly to the HC dimer and dynein LCs assemble on the IC dimer
GO:0045505 dynein intermediate chain binding	IEA GO_REF:0000002	ACCEPT	Summary: Dynein intermediate chain binding. Reason: The heavy chain tail binds the intermediate chains directly (UniProt maps DYNC1I2 interaction to residues 448-703). Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt The ICs and LICs bind directly to the HC dimer and dynein LCs assemble on the IC dimer
GO:0051293 establishment of spindle localization	IMP PMID:23027904 Cortical dynein is critical for proper spindle positioning i...	ACCEPT	Summary: Establishment of spindle localization (IMP, HeLa DYNC1H1 siRNA). Reason: Depletion of DYNC1H1 randomizes spindle position in HeLa cells, and membrane-targeted dynein is sufficient to direct spindle positioning; cortical dynein performs the pulling work. Supporting Evidence: PMID:23027904 or of the dynein heavy chain DYNC1H1 results in the spindle adopting a significantly more random position PMID:23027904 we demonstrated that the mere presence of dynein at that location is sufficient to direct spindle positioning in HeLa cells.
GO:0051959 dynein light intermediate chain binding	IBA GO_REF:0000033	ACCEPT	Summary: Dynein light intermediate chain binding. Reason: The LIC G-domain binds the heavy chain directly via a conserved aromatic patch; DYNC1LI1 and DYNC1LI2 bind mutually exclusively. Supporting Evidence: PMID:25272277 We show that the LIC G domain binds the dynein heavy chain using a conserved patch of aromatic residues file:human/DYNC1H1/DYNC1H1-uniprot.txt DYNC1LI1 and DYNC1LI2 bind mutually exclusive to DYNC1H1
GO:0051959 dynein light intermediate chain binding	IEA GO_REF:0000120	ACCEPT	Summary: Dynein light intermediate chain binding. Reason: The LIC G-domain binds the heavy chain directly via a conserved aromatic patch; DYNC1LI1 and DYNC1LI2 bind mutually exclusively. Supporting Evidence: PMID:25272277 We show that the LIC G domain binds the dynein heavy chain using a conserved patch of aromatic residues file:human/DYNC1H1/DYNC1H1-uniprot.txt DYNC1LI1 and DYNC1LI2 bind mutually exclusive to DYNC1H1
GO:0051959 dynein light intermediate chain binding	IPI PMID:25272277 A Ras-like domain in the light intermediate chain bridges th...	ACCEPT	Summary: Dynein light intermediate chain binding. Reason: The LIC G-domain binds the heavy chain directly via a conserved aromatic patch; DYNC1LI1 and DYNC1LI2 bind mutually exclusively. Supporting Evidence: PMID:25272277 We show that the LIC G domain binds the dynein heavy chain using a conserved patch of aromatic residues file:human/DYNC1H1/DYNC1H1-uniprot.txt DYNC1LI1 and DYNC1LI2 bind mutually exclusive to DYNC1H1
GO:0060236 regulation of mitotic spindle organization	IMP PMID:23027904 Cortical dynein is critical for proper spindle positioning i...	MODIFY	Summary: Regulation of mitotic spindle organization (IMP) from a spindle-positioning study. Reason: The paper measures spindle position/oscillation after DYNC1H1 depletion and cortical dynein targeting, not the organization (assembly/structure) of the spindle. The phenotype is spindle positioning, better captured by establishment of mitotic spindle localization. Proposed replacements: establishment of mitotic spindle localization Supporting Evidence: PMID:23027904 or of the dynein heavy chain DYNC1H1 results in the spindle adopting a significantly more random position PMID:23027904 we demonstrated that the mere presence of dynein at that location is sufficient to direct spindle positioning in HeLa cells.
GO:0070062 extracellular exosome	HDA PMID:19056867 Large-scale proteomics and phosphoproteomics of urinary exos...	MARK AS OVER ANNOTATED	Summary: Extracellular exosome (HDA) from exosome proteomics. Reason: Abundant cytoplasmic proteins, including dynein heavy chain, are routinely recovered in exosome preparations. There is no evidence that exosomal packaging is part of DYNC1H1 function. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasm, cytoskeleton
GO:0070062 extracellular exosome	HDA PMID:19199708 Proteomic analysis of human parotid gland exosomes by multid...	MARK AS OVER ANNOTATED	Summary: Extracellular exosome (HDA) from exosome proteomics. Reason: Abundant cytoplasmic proteins, including dynein heavy chain, are routinely recovered in exosome preparations. There is no evidence that exosomal packaging is part of DYNC1H1 function. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasm, cytoskeleton
GO:0070062 extracellular exosome	HDA PMID:20458337 MHC class II-associated proteins in B-cell exosomes and pote...	MARK AS OVER ANNOTATED	Summary: Extracellular exosome (HDA) from exosome proteomics. Reason: Abundant cytoplasmic proteins, including dynein heavy chain, are routinely recovered in exosome preparations. There is no evidence that exosomal packaging is part of DYNC1H1 function. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasm, cytoskeleton
GO:0070062 extracellular exosome	HDA PMID:21362503 Protein profile of exosomes from trabecular meshwork cells.	MARK AS OVER ANNOTATED	Summary: Extracellular exosome (HDA) from exosome proteomics. Reason: Abundant cytoplasmic proteins, including dynein heavy chain, are routinely recovered in exosome preparations. There is no evidence that exosomal packaging is part of DYNC1H1 function. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasm, cytoskeleton
GO:0070062 extracellular exosome	HDA PMID:23533145 In-depth proteomic analyses of exosomes isolated from expres...	MARK AS OVER ANNOTATED	Summary: Extracellular exosome (HDA) from exosome proteomics. Reason: Abundant cytoplasmic proteins, including dynein heavy chain, are routinely recovered in exosome preparations. There is no evidence that exosomal packaging is part of DYNC1H1 function. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasm, cytoskeleton
GO:0090235 regulation of metaphase plate congression	IMP PMID:27462074 Nuclear Mitotic Apparatus (NuMA) Interacts with and Regulate...	KEEP AS NON CORE	Summary: Regulation of metaphase plate congression (IMP, abstract-only). Reason: UniProt records a role in metaphase plate congression from this paper; dynein at kinetochores and poles contributes to chromosome alignment. This is one of many mitotic roles of the motor. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Plays a role in mitotic spindle assembly and metaphase plate congression PMID:27462074 we found that cytoplasmic dynein is required for the spindle pole accumulation of Astrin, and dynein-mediated transport is important for balanced distribution of Astrin between spindle poles and kinetochores.
GO:0090267 positive regulation of mitotic cell cycle spindle assembly checkpoint	NAS PMID:19229290 Dynein light intermediate chain 1 is required for progress t...	MODIFY	Summary: Positive regulation of mitotic spindle assembly checkpoint (NAS) from a LIC1 study. Reason: The cited work states that dynein removes checkpoint proteins (Mad1/2, Zw10) from kinetochores to silence the spindle assembly checkpoint. That is a role in checkpoint deactivation, the opposite direction to positive regulation. Proposed replacements: deactivation of mitotic spindle assembly checkpoint Supporting Evidence: PMID:19229290 Cytoplasmic dynein functions in the checkpoint, apparently by moving critical checkpoint components off kinetochores. PMID:19229290 We conclude that a Cdk1 phosphorylated form of LIC1 is required to remove Mad1/2 and Zw10 but not BubR1 from kinetochores during spindle assembly checkpoint silencing.
GO:0120162 positive regulation of cold-induced thermogenesis	IEA GO_REF:0000107	MARK AS OVER ANNOTATED	Summary: Positive regulation of cold-induced thermogenesis, from mouse Loa/Cra1 dynein mutant adipose phenotypes. Reason: Dynein mutant mice show cold intolerance and impaired norepinephrine-stimulated lipolysis, attributed to oxidative stress. This is an indirect, pleiotropic physiological phenotype of a ubiquitous motor, not a process the dynein heavy chain executes. Supporting Evidence: PMID:20887786 our results provide genetic evidence that dynein plays a key role in lipid metabolism and thermogenesis through a modulation of oxidative stress elicited by norepinephrine.
GO:0120162 positive regulation of cold-induced thermogenesis	ISS PMID:20887786 Mutations in cytoplasmic dynein lead to a Huntington's disea...	MARK AS OVER ANNOTATED	Summary: Positive regulation of cold-induced thermogenesis, from mouse Loa/Cra1 dynein mutant adipose phenotypes. Reason: Dynein mutant mice show cold intolerance and impaired norepinephrine-stimulated lipolysis, attributed to oxidative stress. This is an indirect, pleiotropic physiological phenotype of a ubiquitous motor, not a process the dynein heavy chain executes. Supporting Evidence: PMID:20887786 our results provide genetic evidence that dynein plays a key role in lipid metabolism and thermogenesis through a modulation of oxidative stress elicited by norepinephrine.
GO:1904115 axon cytoplasm	IEA GO_REF:0000108	ACCEPT	Summary: Axon cytoplasm (IEA inferred from retrograde axonal transport). Reason: Dynein operates in the axoplasm as the retrograde motor. Supporting Evidence: PMID:21820100 Cytoplasmic dynein is a large multisubunit motor protein complex and has a key role in retrograde axonal transport in neurons.
GO:1905832 positive regulation of spindle assembly	IMP PMID:27462074 Nuclear Mitotic Apparatus (NuMA) Interacts with and Regulate...	KEEP AS NON CORE	Summary: Positive regulation of spindle assembly (IMP, abstract-only). Reason: Dynein contributes directly to spindle assembly (see mitotic spindle organization); UniProt records a role in spindle assembly from this paper. Retained as non-core mitotic role. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Plays a role in mitotic spindle assembly and metaphase plate congression

Gene Description

Existing Annotations Review

Core Functions

Catalytic heavy chain of cytoplasmic dynein 1: ATP-driven minus-end-directed movement along microtubules, activated for processive transport by dynactin and cargo adaptors; carries vesicles, organelles and other cargo toward the cell centre, including retrograde axonal transport.

Dynein-driven nuclear movement toward the centrosome (nucleokinesis) in migrating neurons and nuclear positioning; the motor pulls the nucleus along perinuclear microtubules.

Mitotic functions of the motor: bipolar spindle assembly and pole focusing, cortical pulling on astral microtubules for spindle positioning, and removal of checkpoint proteins from kinetochores.

References

Suggested Questions for Experts

Suggested Experiments

Deep Research

Falcon

📚 Additional Documentation

Notes

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