DYNC1H1 encodes the force-generating heavy chain of cytoplasmic dynein-1. Its motor region couples ATP hydrolysis to movement toward microtubule minus ends, while its tail assembles with accessory subunits that connect the motor to cargo and regulatory proteins. Dynein-1 transports organelles and other cellular cargo, including cargo moving toward neuronal cell bodies, and supplies forces for nuclear movement and mitotic spindle organization. Recruitment to the cell cortex helps position the spindle. Long-range processive movement is regulated by dynactin and activating adaptors; the isolated motor is not constitutively highly processive.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0001673 male germ cell nucleus | NAS PMID:36973253 Distinct dynein complexes defined by DYNLRB1 and DYNLRB2 reg... | KEEP AS NON CORE | Summary: Mouse meiotic nuclear-envelope/telomere-associated dynein supports a contextual male-germ-cell nuclear location. Reason: PMID:36973253 Supplementary Figure 4A directly stains DYNC1H1 at telomere-associated sites in mouse pachytene spermatocytes; the original also discusses dynein on the cytoplasmic face of the nuclear envelope. Nuclear envelope is part of nucleus, so an outer-face pool does not by itself refute this broad anatomical assertion. Retain the NAS annotation with explicit mouse-ortholog and nuclear-envelope scope; neither human intranucleoplasmic localization nor a nuclear catalytic activity is established. Supporting Evidence: PMID:36973253 the localization of the dynein–dynactin subunits DYNC1H1, DYNLL1, and p150 at spindle poles were not affected in Dynlrb2−⁄− spermatocytes file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasm, cytoskeleton file:human/DYNC1H1/DYNC1H1-source-evidence.md |
| GO:0003723 RNA binding | HDA PMID:22658674 Insights into RNA biology from an atlas of mammalian mRNA-bi... | KEEP AS NON CORE | Summary: Human HeLa UV-crosslinking and oligo(dT) capture positively detect DYNC1H1 in the mRNA interactome. Reason: The original PMID:22658674 Table S1 contains ENSG00000197102/DYNC1H1 (physical row 809), with ion-count adjusted P=0.00162134388789347 and presence in crosslinked material but not its control. The original Methods use complementary UV protocols, stringent oligo(dT) purification and RNase/proteomic analysis. This is positive RNA-contact evidence despite the absence of a conventional RNA-binding domain; it does not identify the contacted transcript, binding site or physiological RNA-recognition mechanism. Retain as a contextual molecular activity rather than treating transport adaptors as disproof. Supporting Evidence: PMID:22658674 Employing two complementary protocols for covalent UV crosslinking of RBPs to RNA file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md file:human/DYNC1H1/DYNC1H1-source-evidence.md |
| GO:0003723 RNA binding | HDA PMID:22681889 The mRNA-bound proteome and its global occupancy profile on ... | KEEP AS NON CORE | Summary: DYNC1H1 is a candidate mRNA-associated protein in the human HEK293 PAR-CL/SILAC study. Reason: The genuine author thesis associated with PMID:22681889 preserves the study Methods and Table S1. Its DYNC1H1 entry is in candidate group III: the reported log2 ratios include -0.46, NA, -2.62, 1.54 and 4.92, so enrichment is not uniform across protocols. The group is a positive candidate class, not an exclusion list. Photo-crosslinking, oligo(dT) purification and non-crosslinked controls support retaining the reported RNA association with lower source-specific confidence; independent human HeLa capture in PMID:22658674 corroborates the broad activity. No sequence-specific RNA function is inferred. Supporting Evidence: PMID:22681889 We developed a photoreactive nucleotide-enhanced UV crosslinking and oligo(dT) purification approach to identify the mRNA-bound proteome file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md file:human/DYNC1H1/DYNC1H1-source-evidence.md |
| GO:0005515 protein binding | IPI PMID:15161933 Comprehensive proteomic analysis of interphase and mitotic 1... | MODIFY | Summary: The verified 14-3-3 zeta association supports 14-3-3 protein binding. Reason: PMID:15161933 uses GST–14-3-3 zeta affinity capture from human HeLa interphase and mitotic extracts, with GST preclearing, stringent washing and specific peptide elution. The author-deposited original and supplementary lists contain cytoplasmic dynein heavy chain with multiple peptides in both conditions, and the literal MINT pair is DYNC1H1–YWHAZ/P63104. GO:0071889 describes binding to this verified partner class. This affinity association does not establish a purified binary interface, a particular phosphosite or regulation of the partner by DYNC1H1. Proposed replacements: 14-3-3 protein binding Supporting Evidence: PMID:15161933 14-3-3-binding proteins were purified from extracts of interphase and mitotic HeLa cells using specific peptide elution from 14-3-3 zeta affinity columns. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:17043677 Disrupted in Schizophrenia 1 Interactome: evidence for the c... | KEEP AS NON CORE | Summary: A verified DISC1 yeast-two-hybrid interaction is retained as a contextual association. Reason: The genuine PMID:17043677 supplementary interaction table lists three DNCH1/XM_040948 clone contexts with DISC1 N-terminal baits in fetal/adult brain libraries, including truncated prey entries. These are positive source-specific interaction records. DISC1 scaffold identity alone does not identify an informative existing binding subclass or a new DYNC1H1 adaptor mechanism. Retain generic binding as non-core under the project policy, without asserting an endogenous full-length pair or transporting DISC1 in vivo. Supporting Evidence: PMID:17043677 we have generated a network of protein-protein interactions (PPIs) around DISC1. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md file:human/DYNC1H1/DYNC1H1-source-evidence.md |
| GO:0005515 protein binding | IPI PMID:17500595 Huntingtin interacting proteins are genetic modifiers of neu... | KEEP AS NON CORE | Summary: The HTT interaction is positive but does not establish a narrower DYNC1H1 binding activity. Reason: The literal PMID:17500595 records include yeast two-hybrid HTT regions 1–450 and 640–1100, alongside separate spoke-expanded pulldown records involving an N-terminal HTT/polyglutamine construct. The source is not three independent confirmations of a native full-length binary pair. Retain the positive association as non-core; HTT does not become an enzyme or cytoskeletal structural protein merely because it regulates transport. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:17548833 Huntingtin facilitates dynein/dynactin-mediated vesicle tran... | KEEP AS NON CORE | Summary: Huntingtin associates with dynein, with direct fragment mapping to the intermediate chain rather than the heavy chain. Reason: The separately recovered original PMID:17548833 shows dynein/huntingtin coassociation, mouse-brain cytosol immunoprecipitation and affinity work with purified bovine dynein. Its mapped HTT 536–698 interaction is with dynein intermediate chain. The DYNC1H1 source assertion can therefore be retained as a dynein-complex association without claiming a purified heavy-chain binding site. Human HeLa heavy-chain depletion also affects intermediate-chain abundance; that perturbation is not a binary-binding assay. Supporting Evidence: PMID:17548833 Htt binds to dynein and acts in a complex along with dynactin and Htt-associated protein-1 to facilitate vesicular transport. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:21163940 Interactome mapping suggests new mechanistic details underly... | UNDECIDED | Summary: The reported PSEN2 association is positive, but the identity of the detected DYNC1H1 species remains unresolved. Reason: PMID:21163940 reports a DYNC1H1–PSEN2 yeast-two-hybrid pair and reciprocal endogenous coimmunoprecipitation in SH-SY5Y cells with nonimmune-IgG controls. Figure 2C labels the DYNC1H1-reactive band at 140 kDa, which does not establish its relationship to the 4,646-residue heavy chain. The recovered Methods do not identify the DYNC1H1 antibody reagent, and the three literal IntAct records do not specify a DYNC1H1 insert or fragment sequence. Reference-sequence ROGIDs authenticate the database entities, not the unresolved experimental species. Preserve the positive source annotation without claiming a wrong-gene experiment; use UNDECIDED until the band or clone identity can be resolved, and do not raise its specificity to protease binding on the present evidence. Supporting Evidence: PMID:21163940 we report 200 high-confidence protein-protein interactions between eight confirmed AD-related genes and 66 candidates. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md file:human/DYNC1H1/DYNC1H1-source-evidence.md |
| GO:0005515 protein binding | IPI PMID:31092558 Rab46 integrates Ca(2+) and histamine signaling to regulate ... | MODIFY | Summary: The assayed Rab46/CRACR2A long isoform is a small-GTPase partner. Reason: PMID:31092558 studies the 731-residue Rab46/CRACR2A product Q9BSW2-2, including the GTP-locked Q604L mutant, in human endothelial cells. Tagged and endogenous reciprocal coimmunoprecipitations, with the stated fixation/lysis conditions, support dynein association during Weibel–Palade-body trafficking. The assayed product contains the Rab GTPase module and fits GO:0031267; shorter CRACR2A products must not be substituted by name. This is partner-class binding, not evidence that DYNC1H1 catalyzes GTP exchange or directly contacts the nucleotide site. Proposed replacements: small GTPase binding Supporting Evidence: PMID:31092558 GTP-bound Rab46 evokes dynein-dependent retrograde transport of a subset of WPBs along microtubules. file:human/DYNC1H1/DYNC1H1-uniprot.txt Interacts with isoform 2 of CRACR2A file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:31413325 HENA, heterogeneous network-based data set for Alzheimer's d... | KEEP AS NON CORE | Summary: The second DISC1 source independently supports a fragment-defined association. Reason: PMID:31413325 describes a sequence-checked human-brain-library yeast-two-hybrid interaction; the literal IntAct record includes DYNC1H1 binding-region coordinates 3451–3538. The original also examines dynein-associated neuronal material. Retain this source-specific association as non-core rather than requiring an unreported purified interface or transferring DISC1 scaffold functions to DYNC1H1. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified B2RAF7 yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. The retained unreviewed accession has no sufficiently established molecular partner class for an informative replacement; a sequence identifier is not an activity assignment. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Its pairwise Y2H evidence is assessed on the same basis as the refined pairs; the unresolved partner class is the distinguishing limitation. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | MODIFY | Summary: The verified WWP2 association supports ubiquitin protein ligase binding. Reason: PMID:32814053 and the curated records identify this positive human pair in pooling, pairwise-retested and array Y2H stages of the same study. WWP2/O00308 is a HECT-type ubiquitin E3 ligase; the verified partner class fits ubiquitin protein ligase binding. The refinement represents the observed partner-class association; it does not transfer the partner’s activity to DYNC1H1, establish a native cellular interaction, or assert a purified full-length interface. The sequence checksum and any separately annotated binding-region features are preserved distinctly. Proposed replacements: ubiquitin protein ligase binding Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | MODIFY | Summary: The verified TCAP association supports cytoskeletal protein binding. Reason: PMID:32814053 and the curated records identify this positive human pair in pooling, pairwise-retested and array Y2H stages of the same study. TCAP/O15273 is the titin-associated sarcomeric Z-disc structural protein telethonin, a cytoskeletal component. The refinement represents the observed partner-class association; it does not transfer the partner’s activity to DYNC1H1, establish a native cellular interaction, or assert a purified full-length interface. The sequence checksum and any separately annotated binding-region features are preserved distinctly. Proposed replacements: cytoskeletal protein binding Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified PPP1R12B isoform 2 yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. PPP1R12B is a noncatalytic myosin-phosphatase regulatory/targeting subunit. Binding that subunit does not by itself establish binding to the catalytic phosphatase enzyme or to myosin. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified SYNCRIP isoform 4 yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. SYNCRIP is an RNA-binding protein. No supported RNA-binding-protein binding term or RNA-recognition mechanism for the heavy chain was established in this bounded term review; its partner’s RNA binding is not transferred to DYNC1H1. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified TIMM17B yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. TIMM17B is a translocase component. The assay does not establish binding to an assembled transmembrane transporter, and a component name does not prove that TIMM17B itself is the whole transporter. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified BAG3 yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. BAG3 is an Hsp70 co-chaperone/scaffold and nucleotide-exchange regulator; that identity alone does not show that the captured partner is a protein-folding chaperone as required by GO:0051087. No folding-client role is imposed on DYNC1H1. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | MODIFY | Summary: The verified CTSH association supports protease binding. Reason: PMID:32814053 and the curated records identify this positive human pair in pooling, pairwise-retested and array Y2H stages of the same study. CTSH/P09668 is the cysteine peptidase cathepsin H. The class refinement does not require that its catalytic site be the interaction interface. The refinement represents the observed partner-class association; it does not transfer the partner’s activity to DYNC1H1, establish a native cellular interaction, or assert a purified full-length interface. The sequence checksum and any separately annotated binding-region features are preserved distinctly. Proposed replacements: protease binding Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified POLR2E yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. POLR2E is a noncatalytic RNA-polymerase subunit. The fragment/ORF interaction does not demonstrate binding to an assembled RNA-polymerase enzyme, so polymerase binding is not inferred solely from the subunit name. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | MODIFY | Summary: The verified NTF3 isoform 2 association supports growth factor binding. Reason: PMID:32814053 and the curated records identify this positive human pair in pooling, pairwise-retested and array Y2H stages of the same study. The sequence-checked NTF3/P20783-2 precursor retains the neurotrophin growth-factor domain. This identifies the partner class; secretion or physiological precursor processing in yeast is not inferred. The refinement represents the observed partner-class association; it does not transfer the partner’s activity to DYNC1H1, establish a native cellular interaction, or assert a purified full-length interface. The sequence checksum and any separately annotated binding-region features are preserved distinctly. Proposed replacements: growth factor binding Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified GADD45A yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. GADD45A is a stress-response regulatory protein; the association does not demonstrate that DYNC1H1 acquires its signaling or DNA-repair activities. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified CRYBB3 yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. The beta-crystallin partner is not identified as a protein-folding chaperone by this evidence. A crystallin-family name is not sufficient for chaperone-binding refinement. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Its pairwise Y2H evidence is assessed on the same basis as the refined pairs; the unresolved partner class is the distinguishing limitation. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | MODIFY | Summary: The verified OTX2 isoform 2 association supports DNA-binding transcription factor binding. Reason: PMID:32814053 and the curated records identify this positive human pair in pooling, pairwise-retested and array Y2H stages of the same study. OTX2/P32243-2 retains the homeobox DNA-binding domain and its established sequence-specific transcription-factor identity. The term describes the partner class, not DYNC1H1 transcriptional regulation. The refinement represents the observed partner-class association; it does not transfer the partner’s activity to DYNC1H1, establish a native cellular interaction, or assert a purified full-length interface. The sequence checksum and any separately annotated binding-region features are preserved distinctly. Proposed replacements: DNA-binding transcription factor binding Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified HTT yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. The interaction record annotates a sufficient HTT region 1–511 and an amino-acid substitution at position 18. The parent-sequence checksum is separate from these construct features. Retain the positive association without asserting an unmodified full-length HTT reagent or a new motor-regulatory activity. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | MODIFY | Summary: The verified RXRG association supports nuclear receptor binding. Reason: PMID:32814053 and the curated records identify this positive human pair in pooling, pairwise-retested and array Y2H stages of the same study. RXRG/P48443 is a retinoid X nuclear receptor, which fits the specific nuclear-receptor-binding definition. The refinement represents the observed partner-class association; it does not transfer the partner’s activity to DYNC1H1, establish a native cellular interaction, or assert a purified full-length interface. The sequence checksum and any separately annotated binding-region features are preserved distinctly. Proposed replacements: nuclear receptor binding Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | MODIFY | Summary: The verified CDK8 isoform 2 association supports protein kinase binding. Reason: PMID:32814053 and the curated records identify this positive human pair in pooling, pairwise-retested and array Y2H stages of the same study. The reconstructed CDK8/P49336-2 product retains the protein-kinase domain; its alternative residue near 370 is outside that domain. The refinement represents the observed partner-class association; it does not transfer the partner’s activity to DYNC1H1, establish a native cellular interaction, or assert a purified full-length interface. The sequence checksum and any separately annotated binding-region features are preserved distinctly. Proposed replacements: protein kinase binding Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified WDR5 yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. WDR5 is a noncatalytic chromatin-complex scaffold. Its membership in histone-methyltransferase complexes does not make the isolated partner a methyltransferase enzyme. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified ASPH isoform 6 yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. The verified short 270-residue product lacks the C-terminal hydroxylase catalytic module. ASPH gene-level enzyme identity therefore cannot justify enzyme binding for this literal partner product. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified EIF2B5 yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. The epsilon subunit participates in eIF2B nucleotide exchange. The verified association does not identify binding to an assembled exchange-factor complex, and the available enzyme-binding definition cannot be assumed to cover every regulator merely from the word catalytic in a complex description. Retain the positive partner association without inventing a GEF-binding term. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified IL16 isoform 2 yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. The record maps a sufficient N-terminal region 1–453, distinct from the processed C-terminal cytokine. Cytokine binding is therefore not assigned from the parent IL16 name. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | MODIFY | Summary: The verified MAPK11 association supports protein kinase binding. Reason: PMID:32814053 and the curated records identify this positive human pair in pooling, pairwise-retested and array Y2H stages of the same study. MAPK11/Q15759 is a protein kinase; the positive interaction and verified class fit protein kinase binding. The refinement represents the observed partner-class association; it does not transfer the partner’s activity to DYNC1H1, establish a native cellular interaction, or assert a purified full-length interface. The sequence checksum and any separately annotated binding-region features are preserved distinctly. Proposed replacements: protein kinase binding Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified SPATA46 yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. The partner’s developmental association does not establish an informative molecular binding class or a reproductive activity for the heavy chain. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified LGALS9C yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. Galectin identity supports a lectin partner, but the bounded ontology search did not establish a suitable more informative binding term. Retain the association without assigning carbohydrate recognition to DYNC1H1. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md file:human/DYNC1H1/DYNC1H1-source-evidence.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified PABIR3 isoform 2 yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. The official product/sequence identifies this partner; its name and poly(A)-binding-protein association do not establish a narrower heavy-chain molecular activity. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified BZW1 isoform 2 yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. BZW1 is a translation regulator, not a demonstrated DNA-binding transcription factor merely because its name contains basic leucine zipper. The association remains non-core. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | MODIFY | Summary: The verified FA2H association supports enzyme binding. Reason: PMID:32814053 and the curated records identify this positive human pair in pooling, pairwise-retested and array Y2H stages of the same study. FA2H/Q7L5A8 is an experimentally characterized fatty-acid hydroxylase. The record’s sufficient binding region 93–372 includes the hydroxylase module 219–361 but omits its N-terminal cytochrome-b5 region. This supports binding to an enzyme-derived catalytic-domain-containing region, not proof that the truncated reagent was enzymatically active or that dynein alters hydroxylation. The refinement represents the observed partner-class association; it does not transfer the partner’s activity to DYNC1H1, establish a native cellular interaction, or assert a purified full-length interface. The sequence checksum and any separately annotated binding-region features are preserved distinctly. Proposed replacements: enzyme binding Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified Q86WV2 yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. The literal 83-residue entry is a COX4I1-related fragment. Binding it does not establish recognition of an intact cytochrome-c oxidase enzyme or transfer respiratory catalysis to dynein. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified TMPRSS6 Q8IU80-2 yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. The independently reconstructed 461-residue product, officially named isoform 3, lacks the canonical S1 protease domain. Protease-binding refinement is not justified by the TMPRSS6 gene name for this assayed short product. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified TMTC1 isoform 4 yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. The literal 227-residue N-terminal product cannot be equated to the full-length glycosyltransferase. Retain the positive interaction without enzyme-binding refinement from the parent gene alone. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | MODIFY | Summary: The verified NEUROG1 association supports DNA-binding transcription factor binding. Reason: PMID:32814053 and the curated records identify this positive human pair in pooling, pairwise-retested and array Y2H stages of the same study. NEUROG1/Q92886 is an established basic helix-loop-helix DNA-binding transcription factor, providing the relevant molecular partner class. The refinement represents the observed partner-class association; it does not transfer the partner’s activity to DYNC1H1, establish a native cellular interaction, or assert a purified full-length interface. The sequence checksum and any separately annotated binding-region features are preserved distinctly. Proposed replacements: DNA-binding transcription factor binding Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified RTP4 yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. The receptor-trafficking partner does not itself establish a receptor or transporter molecular class in this assay. The association does not assign receptor transport to the heavy chain. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified ZHX1-C8orf76/Q96EF9 yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. The exact 292-residue readthrough product has no independently established DNA-binding transcription-factor function in the inspected official record. It must not be equated to the full-length ZHX1 product from a similar name. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified PLAC8/Q96EJ4 yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. The literal unreviewed record does not establish a sufficiently specific molecular partner class. Retain the positive screen interaction without assigning an activity from its label. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified PRAM1 yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. The partner is a signaling adaptor; association alone does not identify a specific DYNC1H1 adaptor reaction or a validated informative binding term. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | MODIFY | Summary: The verified MAP3K5 association supports protein kinase binding. Reason: PMID:32814053 and the curated records identify this positive human pair in pooling, pairwise-retested and array Y2H stages of the same study. MAP3K5/Q99683 is a protein kinase; the association does not need to map to its catalytic cleft to fit kinase binding. The refinement represents the observed partner-class association; it does not transfer the partner’s activity to DYNC1H1, establish a native cellular interaction, or assert a purified full-length interface. The sequence checksum and any separately annotated binding-region features are preserved distinctly. Proposed replacements: protein kinase binding Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified DCUN1D5 yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. DCUN1D5 is a NEDD8-ligation cofactor, not automatically a ubiquitin E3 ligase. The screen does not justify ubiquitin-ligase binding by transferring a whole-complex activity to its noncatalytic cofactor. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified TEX12 yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. Synaptonemal-complex membership is not equivalent to an actin, microtubule or intermediate-filament cytoskeletal component under GO:0008092. The assay remains positive without that subclass. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified BEX2 yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. The BEX2 association does not establish a specific enzyme, cytoskeletal component or transcription-factor class appropriate to the available binding terms. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified SCAPER isoform 3 yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. The record annotates a sufficient region 863–1154. The parent sequence and this region are distinct evidence fields; neither establishes a new catalytic, transcriptional or native full-length interaction mechanism for DYNC1H1. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | MODIFY | Summary: The verified ARFGAP3 association supports GTPase activating protein binding. Reason: PMID:32814053 and the curated records identify this positive human pair in pooling, pairwise-retested and array Y2H stages of the same study. ARFGAP3/Q9NP61 is an ARF GTPase-activating protein. The definition is binding to that regulator, not binding to ARF or catalyzing GTP hydrolysis. The refinement represents the observed partner-class association; it does not transfer the partner’s activity to DYNC1H1, establish a native cellular interaction, or assert a purified full-length interface. The sequence checksum and any separately annotated binding-region features are preserved distinctly. Proposed replacements: GTPase activating protein binding Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | KEEP AS NON CORE | Summary: The verified WBP1L yeast-two-hybrid association is retained as non-core. Reason: PMID:32814053 and the exact human interaction records support this pair. Three linked records represent the study’s screening stages, not three independent publications. The partner’s signaling-associated identity does not establish a supported narrower molecular binding class or an intrinsic heavy-chain signaling activity. A matching ROGID authenticates the annotated reference sequence, not necessarily every residue of the experimental ORF. The project policy retains supported generic binding where no evidence-backed refinement has been established. Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | MODIFY | Summary: The verified CFL2 association supports cytoskeletal protein binding. Reason: PMID:32814053 and the curated records identify this positive human pair in pooling, pairwise-retested and array Y2H stages of the same study. CFL2/Q9Y281 is the actin-associated cofilin-2 cytoskeletal protein. This association supports the broad cytoskeletal-protein class, not necessarily direct actin binding by the heavy chain. The refinement represents the observed partner-class association; it does not transfer the partner’s activity to DYNC1H1, establish a native cellular interaction, or assert a purified full-length interface. The sequence checksum and any separately annotated binding-region features are preserved distinctly. Proposed replacements: cytoskeletal protein binding Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | MODIFY | Summary: The verified ATP23 association supports protease binding. Reason: PMID:32814053 and the curated records identify this positive human pair in pooling, pairwise-retested and array Y2H stages of the same study. ATP23/Q9Y6H3 is classified as a peptidase M76 protein with the conserved active-site/metal-binding residues and a metalloendopeptidase annotation. That inferred partner classification supports protease binding; the source does not measure human ATP23 catalysis or DYNC1H1-dependent substrate processing. The refinement represents the observed partner-class association; it does not transfer the partner’s activity to DYNC1H1, establish a native cellular interaction, or assert a purified full-length interface. The sequence checksum and any separately annotated binding-region features are preserved distinctly. Proposed replacements: protease binding Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | MODIFY | Summary: The verified DUSP10 association supports protein phosphatase binding. Reason: PMID:32814053 and the curated records identify this positive human pair in pooling, pairwise-retested and array Y2H stages of the same study. DUSP10/Q9Y6W6 is a dual-specificity protein phosphatase and fits the protein-phosphatase-binding definition. The refinement represents the observed partner-class association; it does not transfer the partner’s activity to DYNC1H1, establish a native cellular interaction, or assert a purified full-length interface. The sequence checksum and any separately annotated binding-region features are preserved distinctly. Proposed replacements: protein phosphatase binding Supporting Evidence: PMID:32814053 generated by systematic yeast two-hybrid interaction screening of ∼500 ND-related proteins file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | MODIFY | Summary: The OpenCell YWHAZ association independently supports 14-3-3 protein binding. Reason: The genuine PMID:35271311 OpenCell interaction CSV contains YWHAZ as bait and a DYNC1H1-containing prey accession group at physical CSV line 29632 (zero-based data index 29630). The system uses endogenous split-fluorescent tagging in HEK293T cells. Preserve the full prey group and measured direction rather than silently converting it to an isoform-specific or reciprocal purified interaction. Together with the verified YWHAZ/P63104 class and independent affinity source PMID:15161933, this supports GO:0071889. Proposed replacements: 14-3-3 protein binding |
| GO:0005515 protein binding | IPI PMID:36692009 Structures of human dynein in complex with the lissencephaly... | KEEP AS NON CORE | Summary: Purified human dynein–LIS1 structural work verifies a specific positive interaction. Reason: PMID:36692009 reconstitutes human dynein motor and LIS1 and resolves LIS1 contact at motor-ring/stalk interfaces. This is stronger than an unverified screen hit, but LIS1/PAFAH1B1 is a noncatalytic regulatory subunit rather than a platelet-activating-factor acetylhydrolase enzyme solely by gene name. Retain generic binding as non-core while the dynein-regulatory mechanism informs the integrated motor description; do not invent an unsupported partner-class term. Supporting Evidence: PMID:36692009 The lissencephaly 1 protein, LIS1, is mutated in type-1 lissencephaly and is a key regulator of cytoplasmic dynein-1. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:36950384 Protein interaction studies in human induced neurons indicat... | KEEP AS NON CORE | Summary: GIGYF1 coassociation is verified in human induced-neuron interactomics. Reason: PMID:36950384 uses endogenous affinity proteomics in human iPSC-derived excitatory neurons, with IgG controls and repeated experiments. The exact GIGYF1/O75420 records support the pair but do not resolve direct from complex-mediated contact or a natural isoform. GIGYF1 is not an enzyme or a DNA-binding transcription factor by virtue of its translational-regulatory role. Retain the positive association as non-core. Supporting Evidence: PMID:36950384 including multiple interactions involving SMARCC2, ELAVL3, DPYSL2, and DYNC1H1. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:36950384 Protein interaction studies in human induced neurons indicat... | MODIFY | Summary: The verified ankyrin-B association supports cytoskeletal protein binding. Reason: The literal PMID:36950384 neuronal affinity-proteomics records join DYNC1H1 and ANK2/Q01484. ANK2 is the ankyrin-B membrane–spectrin cytoskeletal linker, fitting GO:0008092. The assay is endogenous human induced-neuron coassociation with controls, not a purified binary interface or an isoform-resolved measurement. The class refinement does not imply that dynein itself builds the spectrin cytoskeleton. Proposed replacements: cytoskeletal protein binding Supporting Evidence: PMID:36950384 including multiple interactions involving SMARCC2, ELAVL3, DPYSL2, and DYNC1H1. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:38547289 Molecular mechanism of dynein-dynactin complex assembly by L... | KEEP AS NON CORE | Summary: Full dynein/LIS1/adaptor reconstitution confirms a regulatory association without a suitable narrower binding class. Reason: PMID:38547289 combines human recombinant dynein and LIS1, dynactin/adaptor assemblies and cellular perturbations to resolve LIS1 contacts and activation states. Preserve these positive mechanistic findings, including distinctions among wild-type and open-state engineered motors. LIS1 is not assigned an enzyme class from its PAFAH1B1 name. Generic binding remains non-core under the project policy; its motor-regulatory context belongs in the integrated motor synthesis. Supporting Evidence: PMID:38547289 Unexpectedly, LIS1 binds dynactin's p150 subunit, tethering it along the length of dynein. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005524 ATP binding | IEA GO_REF:0000002 | ACCEPT | Summary: ATP binding is intrinsic to the dynein AAA+ motor. Reason: The IPR035699-based electronic assertion is consistent with the verified DYNC1H1 AAA+ motor architecture and human ATP-driven motility experiments. ATP binding supports the motor’s nucleotide cycle rather than a separate ATP-binding core. Propagation Review Root cause: NO FAILURE CORE Sources checked: InterPro:IPR035699 SUPPORTS TRANSFER The imported domain/family mapping is corroborated by the human motor’s ATP-dependent activity; no source-field change is made. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-6798751 | KEEP AS NON CORE | Summary: Extracellular detection is retained with separate model and proteomic evidence scopes. Reason: The exact Reactome degranulation chain contains DYNC1H1 granule-lumen entity 6799155 and extracellular output entity 6806223, each mapped to human Q14204 residues 1–4646. This is curated model membership, not an independently recovered neutrophil target assay. Genuine human parotid-exosome Table 2 in PMID:19199708 and the urinary-exosome database linked to PMID:19056867 independently detect DYNC1H1 in extracellular preparations. Retain the broad extracellular location as non-core without inferring classical secretion, vesicle biogenesis or resolving the narrower granule-lumen claim. Absence of a signal peptide is not a disproof. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasm, cytoskeleton file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md file:human/DYNC1H1/DYNC1H1-source-evidence.md |
| GO:0005737 cytoplasm | IEA GO_REF:0000107 | ACCEPT | Summary: The conserved cytoplasmic motor localization is retained. Reason: The imported orthology mapping names mouse Dync1h1/Q9JHU4. The genuine mouse annotation record retains a cytoplasmic IDA source, and independent human recombinant/cellular motor evidence and the exact human Reactome cytosolic entities support the broad cytoplasmic pool. This does not exclude membrane-associated, cortical or other nonexclusive subcellular pools. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:Q9JHU4 SUPPORTS TRANSFER Current mouse source record retains cytoplasmic localization; human target evidence independently supports transfer. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasm, cytoskeleton file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. |
| GO:0005813 centrosome | IDA PMID:21399614 Novel asymmetrically localizing components of human centroso... | ACCEPT | Summary: Centrosomal localization is supported at target level while the screen-specific table remains unresolved. Reason: PMID:21399614 describes human KE37 centrosome enrichment/protein-correlation profiling and cellular validation, but the target-specific supplementary record was not independently recovered. This is not grounds to reject the curator’s experimental assertion: independent human DYNC1H1 centrosome/cell imaging in PMID:28602352 supports the same location. Retain centrosome without claiming that this particular proteomic row or every validation image was inspected. Supporting Evidence: PMID:21399614 we distinguished 126 known and 40 candidate centrosomal proteins |
| GO:0005813 centrosome | IDA PMID:28602352 Cryo-EM Reveals How Human Cytoplasmic Dynein Is Auto-inhibit... | ACCEPT | Summary: Human DYNC1H1 centrosomal localization is supported by the original cellular experiments. Reason: PMID:28602352 includes human-cell localization of tagged dynein constructs and centrosomal/spindle observations. Keep the wild-type and engineered open-state experiments distinct and do not interpret all localization changes as the behavior of unmodified endogenous protein. These experiments support a functional centrosomal pool. Supporting Evidence: PMID:28602352 GFP-mtDyn accumulates more at the centrosomes than GFP-wtDyn. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-141409 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in Mad1 binds kinetochore. Reason: The actual Reactome entity chain is 141409 input 375305; 375305 hasComponent 2029145; 2029145 hasComponent 2029144; 2029144 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-141422 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in MAD2 converted to an inhibitory state via interaction with Mad1. Reason: The actual Reactome entity chain is 141422 input 141427; 141427 hasComponent 141441; 141441 hasComponent 375305; 375305 hasComponent 2029145; 2029145 hasComponent 2029144; 2029144 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-141431 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in MAD2 associates with the Mad1 kinetochore complex. Reason: The actual Reactome entity chain is 141431 input 141441; 141441 hasComponent 375305; 375305 hasComponent 2029145; 2029145 hasComponent 2029144; 2029144 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-141439 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in Release of activated MAD2 from kinetochores. Reason: The actual Reactome entity chain is 141439 output 141441; 141441 hasComponent 375305; 375305 hasComponent 2029145; 2029145 hasComponent 2029144; 2029144 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-1638803 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in Phosphorylation of cohesin by PLK1 at centromeres. Reason: The actual Reactome entity chain is 1638803 input 2468282; 2468282 hasComponent 375303; 375303 hasComponent 375305; 375305 hasComponent 2029145; 2029145 hasComponent 2029144; 2029144 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-1638821 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in PP2A-B56 dephosphorylates centromeric cohesin. Reason: The actual Reactome entity chain is 1638821 input 1638796; 1638796 hasComponent 375303; 375303 hasComponent 375305; 375305 hasComponent 2029145; 2029145 hasComponent 2029144; 2029144 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-2213248 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in Transport of antigen loaded MHC II molecules to surface. Reason: The actual Reactome entity chain is 2213248 input 2029135; 2029135 hasComponent 2029145; 2029145 hasComponent 2029144; 2029144 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-2467809 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in ESPL1 (Separase) cleaves centromeric cohesin. Reason: The actual Reactome entity chain is 2467809 output 2467806; 2467806 hasComponent 375305; 375305 hasComponent 2029145; 2029145 hasComponent 2029144; 2029144 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-2467811 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in Separation of sister chromatids. Reason: The actual Reactome entity chain is 2467811 input 2467806; 2467806 hasComponent 375305; 375305 hasComponent 2029145; 2029145 hasComponent 2029144; 2029144 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-2468287 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in CDK1 phosphorylates CDCA5 (Sororin) at centromeres. Reason: The actual Reactome entity chain is 2468287 input 2484819; 2484819 hasComponent 2484816; 2484816 hasComponent 375305; 375305 hasComponent 2029145; 2029145 hasComponent 2029144; 2029144 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-2484822 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in Kinetochore assembly. Reason: The actual Reactome entity chain is 2484822 input 375303; 375303 hasComponent 375305; 375305 hasComponent 2029145; 2029145 hasComponent 2029144; 2029144 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-2574840 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in AJUBA facilitates AURKA autophosphorylation. Reason: The actual Reactome entity chain is 2574840 output 3000302; 3000302 hasComponent 380268; 380268 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-2574845 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in AJUBA binds centrosome-associated AURKA. Reason: The actual Reactome entity chain is 2574845 input 2574827; 2574827 hasComponent 380268; 380268 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-3000310 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in AURKA phosphorylates PLK1. Reason: The actual Reactome entity chain is 3000310 output 3000302; 3000302 hasComponent 380268; 380268 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-3000319 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in BORA binds PLK1 and AURKA. Reason: The actual Reactome entity chain is 3000319 input 3000302; 3000302 hasComponent 380268; 380268 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-375302 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in Kinetochore capture of astral microtubules. Reason: The actual Reactome entity chain is 375302 input 375305; 375305 hasComponent 2029145; 2029145 hasComponent 2029144; 2029144 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-380272 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in Plk1-mediated phosphorylation of Nlp. Reason: The actual Reactome entity chain is 380272 input 380268; 380268 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-380283 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in Recruitment of additional gamma tubulin/ gamma TuRC to the centrosome. Reason: The actual Reactome entity chain is 380283 input 380268; 380268 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-380294 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in Loss of C-Nap-1 from centrosomes. Reason: The actual Reactome entity chain is 380294 input 380268; 380268 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-380303 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in Dissociation of Phospho-Nlp from the centrosome. Reason: The actual Reactome entity chain is 380303 input 380704; 380704 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-380311 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in Recruitment of Plk1 to centrosomes. Reason: The actual Reactome entity chain is 380311 input 380268; 380268 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-380316 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in Association of NuMA with microtubules. Reason: The actual Reactome entity chain is 380316 input 380503; 380503 hasComponent 380440; 380440 hasComponent 380268; 380268 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-380455 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in Recruitment of CDK11p58 to the centrosomes. Reason: The actual Reactome entity chain is 380455 input 380268; 380268 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-380508 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in Translocation of NuMA to the centrosomes. Reason: The actual Reactome entity chain is 380508 input 380440; 380440 hasComponent 380268; 380268 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5617816 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in RAB3IP stimulates nucleotide exchange on RAB8A . Reason: The actual Reactome entity chain is 5617816 catalystActivity 5617818; 5617818 physicalEntity 5637989; 5637989 hasComponent 5626673; 5626673 hasComponent 5626181; 5626181 hasComponent 5626173; 5626173 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5626220 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in C2CD3 binds the mother centriole. Reason: The actual Reactome entity chain is 5626220 input 5626171; 5626171 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5626223 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in C2CD3 and OFD1 recruit 5 distal appendage proteins to the centriole. Reason: The actual Reactome entity chain is 5626223 input 5626175; 5626175 hasComponent 5626171; 5626171 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5626227 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in CP110 and CEP97 dissociate from the centriole. Reason: The actual Reactome entity chain is 5626227 output 5626181; 5626181 hasComponent 5626173; 5626173 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5626228 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in The distal appendage proteins recruit TTBK2. Reason: The actual Reactome entity chain is 5626228 input 5626176; 5626176 hasComponent 5626175; 5626175 hasComponent 5626171; 5626171 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5626681 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in Recruitment of transition zone proteins. Reason: The actual Reactome entity chain is 5626681 input 5626181; 5626181 hasComponent 5626173; 5626173 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5626699 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in MARK4 binds ODF2 in the centriole. Reason: The actual Reactome entity chain is 5626699 input 5626178; 5626178 hasComponent 5626176; 5626176 hasComponent 5626175; 5626175 hasComponent 5626171; 5626171 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5638009 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in CEP164 recruits RAB3IP-carrying Golgi-derived vesicles to the basal body. Reason: The actual Reactome entity chain is 5638009 input 5626673; 5626673 hasComponent 5626181; 5626181 hasComponent 5626173; 5626173 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5666129 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in CDC42:GTP recruits DIAPH2-2 to kinetochores. Reason: The actual Reactome entity chain is 5666129 input 375305; 375305 hasComponent 2029145; 2029145 hasComponent 2029144; 2029144 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5666160 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in AURKB phosphorylates DIAPH2-2 at kinetochores. Reason: The actual Reactome entity chain is 5666160 input 5666131; 5666131 hasComponent 375305; 375305 hasComponent 2029145; 2029145 hasComponent 2029144; 2029144 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5666169 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in Kinetochore capture of astral microtubules is positively regulated by CDC42:GTP:p-S196-DIAPH2-2. Reason: The actual Reactome entity chain is 5666169 input 375305; 375305 hasComponent 2029145; 2029145 hasComponent 2029144; 2029144 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-6809003 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in ERGIC-to-Golgi vesicles bind dynein:dynactin . Reason: The actual Reactome entity chain is 6809003 input 2029135; 2029135 hasComponent 2029145; 2029145 hasComponent 2029144; 2029144 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-6809006 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in Vesicle is tethered through binding GOLGA2:GORASP1, GOLGB1 and the COG complex. Reason: The actual Reactome entity chain is 6809006 output 2029135; 2029135 hasComponent 2029145; 2029145 hasComponent 2029144; 2029144 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8849350 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in RAB6:GTP displaces PAFAH1B1 from dynein:dynactin complex. Reason: The actual Reactome entity chain is 8849350 input 8849246; 8849246 hasComponent 2029135; 2029135 hasComponent 2029145; 2029145 hasComponent 2029144; 2029144 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8849353 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in Dynein drives COPI-independent retrograde traffic from the Golgi to the ER. Reason: The actual Reactome entity chain is 8849353 output 2029135; 2029135 hasComponent 2029145; 2029145 hasComponent 2029144; 2029144 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8853405 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in TPX2 binds AURKA at centrosomes. Reason: The actual Reactome entity chain is 8853405 input 2574827; 2574827 hasComponent 380268; 380268 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8853419 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in TPX2 promotes AURKA autophosphorylation. Reason: The actual Reactome entity chain is 8853419 input 8853414; 8853414 hasComponent 2574827; 2574827 hasComponent 380268; 380268 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9614343 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in Viral UL47:UL48 Proteins Bind HCMV Tegumented Virion to Host Microtuble and Dynein complexs. Reason: The actual Reactome entity chain is 9614343 input 2029145; 2029145 hasComponent 2029144; 2029144 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9614367 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in HCMV Nuclear Pore Docking. Reason: The actual Reactome entity chain is 9614367 output 2029145; 2029145 hasComponent 2029144; 2029144 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9646348 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in PolyUb-Misfolded Proteins:HDAC6 bind dynein motor. Reason: The actual Reactome entity chain is 9646348 input 2029145; 2029145 hasComponent 2029144; 2029144 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9646679 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in PolyUb-Misfolded proteins bind vimentin to form aggresome. Reason: The actual Reactome entity chain is 9646679 input 9646389; 9646389 hasComponent 9646395; 9646395 hasComponent 2029145; 2029145 hasComponent 2029144; 2029144 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9646685 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in Aggresome dissociates from dynein and microtubule. Reason: The actual Reactome entity chain is 9646685 output 2029145; 2029145 hasComponent 2029144; 2029144 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9648114 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in EML4 recruits NUDC to mitotic spindle. Reason: The actual Reactome entity chain is 9648114 input 375303; 375303 hasComponent 375305; 375305 hasComponent 2029145; 2029145 hasComponent 2029144; 2029144 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005829 cytosol | TAS Reactome:R-NUL-9646387 | ACCEPT | Summary: Human cytosolic DYNC1H1 is explicitly represented in Dynein motors transport misfolded proteins. Reason: The actual Reactome entity chain is 9646387 input 9646384; 9646384 hasComponent 2029145; 2029145 hasComponent 2029144; 2029144 hasComponent 380285, ending at DYNC1H1 entity 380285 in cytosol, mapped to human Q14204 residues 1–4646. The heavy chain is represented in the dynein complex/dimer rather than inferred solely from a pathway title. Retain the cytosolic location without implying that each event independently assays free soluble protein. The R-NUL event is a chimeric model; the selected DYNC1H1 leaf itself remains human. The original source identifier is preserved. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005856 cytoskeleton | IEA GO_REF:0000044 | MODIFY | Summary: The broad cytoskeleton mapping can be refined to the established cytoplasmic microtubule pool. Reason: The UniProt subcellular-location mapping supports cytoskeletal localization. Independent human motor assays and spindle/cortical transport studies identify the functional cytoplasmic microtubule-associated pool more specifically. GO:0005881 is the target-level refinement; this does not claim that the electronic mapping itself measured that narrower location or exclude other associated pools. Propagation Review Root cause: TERM SCOPING PROBLEM Sources checked: UniProtKB-SubCell:SL-0090 SUPPORTS TRANSFER The broad source location is valid; human motor evidence supports a more specific cytoplasmic microtubule location. Proposed replacements: cytoplasmic microtubule Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasm, cytoskeleton |
| GO:0005868 cytoplasmic dynein complex | IBA GO_REF:0000033 | ACCEPT | Summary: Cytoplasmic dynein-complex membership is inherited and directly corroborated in human. Reason: The literal PAINT IBD at PTN000072732 and the recovered PTHR10676 target descent support cytoplasmic dynein membership. The target itself legitimately occurs among descendant evidences; that is not circularity. Human recombinant heavy-chain complexes in PMID:21723285 and PMID:24986880 independently corroborate the conserved assembly. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000072732 SUPPORTS TRANSFER The exact IBD row and human target descent were independently reconstructed from the current PTHR10676 tree/MSA and literal PAINT source. No target-specific functional loss was established; donor count and target self-grounding are not failure criteria. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt The ICs and LICs bind directly to the HC dimer and dynein LCs assemble on the IC dimer file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005868 cytoplasmic dynein complex | IDA PMID:21723285 Recombinant human cytoplasmic dynein heavy chain 1 and 2: ob... | ACCEPT | Summary: Human recombinant DYNC1H1 assembles into cytoplasmic dynein. Reason: PMID:21723285 analyzes human dynein complexes expressed in HEK293 cells. Associated chains and the heavy-chain motor together establish complex membership; the source’s ensemble microtubule-gliding result is not itself a single-complex processivity measurement. Supporting Evidence: PMID:21723285 In an in vitro MT gliding assay, both dynein-1 and dynein-2 showed minus-end-directed motor activities. file:human/DYNC1H1/DYNC1H1-uniprot.txt The ICs and LICs bind directly to the HC dimer and dynein LCs assemble on the IC dimer |
| GO:0005868 cytoplasmic dynein complex | IDA PMID:25272277 A Ras-like domain in the light intermediate chain bridges th... | ACCEPT | Summary: Human heavy-chain association with the LIC domain supports dynein-complex assembly. Reason: PMID:25272277 analyzes human DYNC1LI1 constructs: full-length LIC1 and its 1–389 G-domain region associate with heavy chain, whereas the 389–523 tail does not support the same assembly. Cellular coimmunoprecipitation and mutational mapping support DYNC1H1 as a dynein-complex subunit. They are not represented as a purified two-protein affinity measurement. Supporting Evidence: PMID:25272277 We show that the LIC G domain binds the dynein heavy chain using a conserved patch of aromatic residues file:human/DYNC1H1/DYNC1H1-uniprot.txt The ICs and LICs bind directly to the HC dimer and dynein LCs assemble on the IC dimer |
| GO:0005868 cytoplasmic dynein complex | IEA GO_REF:0000117 | ACCEPT | Summary: The electronic cytoplasmic dynein-complex assertion is independently supported. Reason: The imported ARBA mapping agrees with the defined human dynein complex and human recombinant assembly evidence. Retain the assertion without treating the electronic rule as an independent experiment. Propagation Review Root cause: NO FAILURE CORE Sources checked: ARBA:ARBA00088183 SUPPORTS TRANSFER The electronic complex-membership claim is corroborated by direct target assembly evidence. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt The ICs and LICs bind directly to the HC dimer and dynein LCs assemble on the IC dimer |
| GO:0005868 cytoplasmic dynein complex | IPI PMID:24986880 In vitro reconstitution of a highly processive recombinant h... | ACCEPT | Summary: Purified recombinant human dynein establishes complex membership. Reason: PMID:24986880 reconstitutes six human dynein subunits expressed in insect cells and analyzes their motility with mouse BICD2N and pig-brain dynactin. Host species must not be substituted for the dynein construct species. Complex membership is direct; sustained processivity requires the stated activating partners. Supporting Evidence: PMID:24986880 Cytoplasmic dynein is an approximately 1.4 MDa multi-protein complex that transports many cellular cargoes towards the minus ends of microtubules. file:human/DYNC1H1/DYNC1H1-uniprot.txt The ICs and LICs bind directly to the HC dimer and dynein LCs assemble on the IC dimer |
| GO:0005868 cytoplasmic dynein complex | NAS PMID:8666668 Mammalian cells express three distinct dynein heavy chains t... | ACCEPT | Summary: The historical dynein-complex statement is retained with its access limit. Reason: The PMID:8666668 cached abstract is available, but the recovered archival XML contains PDF links rather than an operative full body. Independent human recombinant assembly in PMID:21723285 and PMID:24986880 establishes this same complex membership, so the correct NAS assertion can be retained without claiming to have inspected the historical Methods. Supporting Evidence: PMID:8666668 We describe two dynein heavy chain (DHC)-like polypeptides (DHCs 2 and 3) that are distinct from the heavy chain of conventional cytoplasmic dynein (DHC1) file:human/DYNC1H1/DYNC1H1-uniprot.txt The ICs and LICs bind directly to the HC dimer and dynein LCs assemble on the IC dimer |
| GO:0005874 microtubule | IDA PMID:21525035 PEX14 is required for microtubule-based peroxisome motility ... | MODIFY | Summary: The established motor-associated location is cytoplasmic microtubule. Reason: The PMID:21525035 abstract foregrounds PEX14–tubulin binding and does not expose the curator’s target-specific DYNC1H1 assay. That is not evidence of misattribution. The motor’s human microtubule-binding/motility evidence independently establishes the cytoplasmic microtubule pool, allowing a gene-level refinement to GO:0005881 while explicitly preserving the source-specific full-text limit. Proposed replacements: cytoplasmic microtubule Supporting Evidence: PMID:21525035 peroxisomal remnants in PEX14-deficient cells have lost their ability to move along microtubules. file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. |
| GO:0005881 cytoplasmic microtubule | IBA GO_REF:0000033 | ACCEPT | Summary: The cytoplasmic microtubule location is a supported ancestral dynein-1 property. Reason: The literal PTN000072732 IBD for cytoplasmic microtubule is present in the current PAINT data and the human target descends from that node. Human motor and spindle experiments independently support the location. No loss or target-specific divergence was found; donor number is not used to grade the curator’s node placement. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000072732 SUPPORTS TRANSFER The exact IBD row and human target descent were independently reconstructed from the current PTHR10676 tree/MSA and literal PAINT source. No target-specific functional loss was established; donor count and target self-grounding are not failure criteria. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0005938 cell cortex | IBA GO_REF:0000033 | ACCEPT | Summary: Cortical dynein localization is conserved and directly supported in human cells. Reason: The literal cell-cortex IBD at PTN000072732 reaches the human target. PMID:23027904 localizes dynein through the G-alpha-i/LGN/NuMA cortical machinery in human HeLa cells and tests its effect on spindle positioning. This is a genuine functional cortical pool, not a claim that all dynein is membrane-bound. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000072732 SUPPORTS TRANSFER The exact IBD row and human target descent were independently reconstructed from the current PTHR10676 tree/MSA and literal PAINT source. No target-specific functional loss was established; donor count and target self-grounding are not failure criteria. Supporting Evidence: PMID:23027904 we demonstrated that the mere presence of dynein at that location is sufficient to direct spindle positioning in HeLa cells. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0007018 microtubule-based movement | IEA GO_REF:0000002 | ACCEPT | Summary: Microtubule-based movement is supported by the motor architecture and direct human activity. Reason: The imported InterPro mapping is consistent with ATP-dependent minus-end movement of human dynein in PMID:21723285 and activated processive transport in PMID:24986880. Preserve this broad biological-process assertion; activation by dynactin/adaptor and ensemble versus single-molecule assays remain distinct. Propagation Review Root cause: NO FAILURE CORE Sources checked: InterPro:IPR004273 SUPPORTS TRANSFER The imported motor-family mapping is independently corroborated by direct human movement assays. Supporting Evidence: PMID:21723285 In an in vitro MT gliding assay, both dynein-1 and dynein-2 showed minus-end-directed motor activities. file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. |
| GO:0007052 mitotic spindle organization | IBA GO_REF:0000033 | ACCEPT | Summary: Mitotic spindle organization is a conserved dynein-1 role. Reason: The literal PTN000072732 IBD and target lineage support the inherited process. Human DYNC1H1 depletion in PMID:27462074 alters spindle-pole organization and transport of pole proteins, while PMID:23027904 demonstrates cortical positioning work. These observations support direct motor participation, not merely an association with mitotic cells. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000072732 SUPPORTS TRANSFER The exact IBD row and human target descent were independently reconstructed from the current PTHR10676 tree/MSA and literal PAINT source. No target-specific functional loss was established; donor count and target self-grounding are not failure criteria. Supporting Evidence: PMID:8227145 Cells become arrested with unseparated centrosomes and form monopolar spindles. file:human/DYNC1H1/DYNC1H1-uniprot.txt Plays a role in mitotic spindle assembly and metaphase plate congression file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0007052 mitotic spindle organization | NAS PMID:8227145 Cytoplasmic dynein plays a role in mammalian mitotic spindle... | ACCEPT | Summary: The historical spindle-organization statement is consistent with direct human motor work. Reason: PMID:8227145 reports antibody perturbations of spindle formation in its accessible abstract; the recovered archival XML does not provide a readable full Methods body. Later human heavy-chain depletion and positioning experiments independently support the process. Retain the NAS assertion with this access boundary, without inventing the historical antibody epitope or species-specific construct. Supporting Evidence: PMID:8227145 Their injection into mitotic mammalian cells blocks the formation of spindles in prophase or during recovery from nocodazole treatment at later stages of mitosis. PMID:8227145 Cells become arrested with unseparated centrosomes and form monopolar spindles. |
| GO:0007097 nuclear migration | IBA GO_REF:0000033 | ACCEPT | Summary: Nuclear migration is retained as an ancestral dynein-1 transport role. Reason: The literal PTN000072732 nuclear-migration IBD is inherited by the human DYNC1H1 sequence in the reconstructed family tree. The assertion is a curator’s ancestral functional placement, not a pairwise inference from donor count. No specific loss or divergence is established for human dynein-1; the motor supplies force for nuclear movement rather than being the transported nucleus itself. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000072732 SUPPORTS TRANSFER The exact IBD row and human target descent were independently reconstructed from the current PTHR10676 tree/MSA and literal PAINT source. No target-specific functional loss was established; donor count and target self-grounding are not failure criteria. Supporting Evidence: PMID:15173193 Dynein inhibition resulted in similar defects in both nucleus-centrosome (N-C) coupling and neuronal migration. PMID:17618279 The nucleus is transported along the trailing microtubules by dynein assisted by myosin II. file:human/DYNC1H1/DYNC1H1-deep-research-falcon.md severe retinal-layer disorganization by postnatal day 6, defective nuclear positioning and membrane-protein trafficking file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0008090 retrograde axonal transport | IBA GO_REF:0000033 | ACCEPT | Summary: Retrograde axonal transport is inherited within the metazoan dynein-1 branch. Reason: The literal PTN000072735 IBD for GO:0008090 is present and the human target descends through that node in PTHR10676. The source node is distinct from the older root dynein assertion and is supported by experimentally annotated metazoan descendants. Preserve the conserved motor-transport role without claiming that a family sequence alone establishes every human cargo. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000072735 SUPPORTS TRANSFER The exact IBD row and human target descent were independently reconstructed from the current PTHR10676 tree/MSA and literal PAINT source. No target-specific functional loss was established; donor count and target self-grounding are not failure criteria. Supporting Evidence: PMID:21820100 Cytoplasmic dynein is a large multisubunit motor protein complex and has a key role in retrograde axonal transport in neurons. PMID:12730604 missense point mutations in the cytoplasmic dynein heavy chain result in progressive motor neuron degeneration in heterozygous mice file:human/DYNC1H1/DYNC1H1-deep-research-falcon.md In polarized neurons, it powers long-range retrograde axonal transport from distal processes toward the soma. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0008569 minus-end-directed microtubule motor activity | IDA PMID:21723285 Recombinant human cytoplasmic dynein heavy chain 1 and 2: ob... | ACCEPT | Summary: Human dynein has experimentally demonstrated minus-end-directed motor activity. Reason: The source explicitly carries contributes_to, which is retained. PMID:21723285 directly measures minus-end-directed microtubule gliding by recombinant human dynein complexes; the heavy chain contains the ATPase/force-producing motor. The ensemble assay is not proof that an isolated heavy-chain molecule is processive. PMID:24986880 distinguishes unactivated human dynein from processive dynein–dynactin–adaptor assemblies. Supporting Evidence: PMID:21723285 In an in vitro MT gliding assay, both dynein-1 and dynein-2 showed minus-end-directed motor activities. PMID:24986880 addition of dynactin together with the N-terminal region of the cargo adaptor BICD2 (BICD2N) gives rise to unidirectional dynein movement over remarkably long distances. file:human/DYNC1H1/DYNC1H1-uniprot.txt Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. |
| GO:0008569 minus-end-directed microtubule motor activity | IEA GO_REF:0000002 | ACCEPT | Summary: The electronic minus-end motor assertion agrees with direct human assays. Reason: The IPR004273 motor-domain mapping is consistent with the human ATP-driven, minus-end-directed dynein activity. The full complex and activating-partner requirements are retained in the biological interpretation; an electronic annotation is not a separate biochemical assay. Propagation Review Root cause: NO FAILURE CORE Sources checked: InterPro:IPR004273 SUPPORTS TRANSFER The motor-domain transfer agrees with directly measured human minus-end motion. Supporting Evidence: PMID:21723285 In an in vitro MT gliding assay, both dynein-1 and dynein-2 showed minus-end-directed motor activities. PMID:24986880 addition of dynactin together with the N-terminal region of the cargo adaptor BICD2 (BICD2N) gives rise to unidirectional dynein movement over remarkably long distances. file:human/DYNC1H1/DYNC1H1-uniprot.txt Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. |
| GO:0016020 membrane | HDA PMID:19946888 Defining the membrane proteome of NK cells. | KEEP AS NON CORE | Summary: Peripheral membrane association is biologically supported, with the proteomic target row still unresolved. Reason: PMID:19946888 is a membrane-fraction proteomics study; the genuine target-specific supplementary table was not recovered, so its precise DYNC1H1 peptides and fraction assignment remain uninspected. Independently, human cortical dynein recruitment by membrane-associated G-alpha-i/LGN/NuMA in PMID:23027904 supports a nonexclusive membrane-associated pool. The broad membrane definition does not require an integral membrane helix. Retain the experimentally curated broad location as contextual, without claiming direct lipid insertion, declaring contamination, or presenting the missing table as recovered. Supporting Evidence: PMID:19946888 The remaining species were largely involved in cellular processes and molecular functions that could be predicted to be transiently associated with membranes. PMID:25272277 We show that the LIC G domain binds the dynein heavy chain using a conserved patch of aromatic residues file:human/DYNC1H1/DYNC1H1-source-evidence.md |
| GO:0030175 filopodium | IEA GO_REF:0000107 | UNDECIDED | Summary: The literal mouse filopodium donor exists, but its specific localization assay remains unresolved. Reason: The current mouse Dync1h1/Q9JHU4 record carries an IDA filopodium annotation from PMID:16496424. The authentic abstract establishes murine Dnali1–Dync1h1 fragment interaction and colocalization, but not the particular filopodial imaging conditions. Publisher and author-thesis retrieval attempts did not yield an operative full text. Preserve the genuine source and its orthology mapping; use UNDECIDED for this narrow location rather than inferring it from general cytoplasmic distribution or declaring the donor wrong. Propagation Review Root cause: UNRESOLVED Sources checked: UniProtKB:Q9JHU4 UNRESOLVED The current mouse IDA/PMID:16496424 filopodium assertion is real, but its target-specific imaging evidence was not recovered. |
| GO:0030286 dynein complex | IEA GO_REF:0000002 | MODIFY | Summary: The broad dynein-complex mapping can be refined to cytoplasmic dynein complex. Reason: The imported motor-family mapping describes dynein membership. Human DYNC1H1 is independently verified in the cytoplasmic dynein complex by recombinant assembly and associated-chain experiments. GO:0005868 is a typed descendant of the imported broad complex term and is the appropriate target-level refinement; it does not turn the mapping into an axonemal-dynein assertion. Propagation Review Root cause: TERM SCOPING PROBLEM Sources checked: InterPro:IPR004273 SUPPORTS TRANSFER The broad family-level dynein membership is correct; direct human complex evidence supports the more specific cytoplasmic complex. Proposed replacements: cytoplasmic dynein complex Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt The ICs and LICs bind directly to the HC dimer and dynein LCs assemble on the IC dimer |
| GO:0031122 cytoplasmic microtubule organization | IBA GO_REF:0000033 | ACCEPT | Summary: Cytoplasmic microtubule organization is a supported conserved dynein-1 role. Reason: The literal PTN000072732 IBD for GO:0031122 reaches the human target. Dynein’s force-producing interactions participate in microtubule organization, and human spindle/cortical experiments are compatible with that conserved role. The inference is not rejected because a selected donor list is short or taxonomically distant. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000072732 SUPPORTS TRANSFER The exact IBD row and human target descent were independently reconstructed from the current PTHR10676 tree/MSA and literal PAINT source. No target-specific functional loss was established; donor count and target self-grounding are not failure criteria. Supporting Evidence: PMID:33957056 Cytoplasmic dynein is responsible for transporting intracellular cargos in interphase cells and mediating spindle assembly and chromosome positioning during cell division. PMID:8227145 Cells become arrested with unseparated centrosomes and form monopolar spindles. file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0032388 positive regulation of intracellular transport | IMP PMID:27462074 Nuclear Mitotic Apparatus (NuMA) Interacts with and Regulate... | MODIFY | Summary: The source identifies transport of spindle-pole proteins along microtubules. Reason: PMID:27462074 uses human HeLa DYNC1H1 depletion and measures reduced pole accumulation of NuMA and Astrin, together with impaired spindle organization. The motor supplies the transport work underlying this phenotype. GO:0098840 captures protein transport along microtubules more directly than the broad positive-regulation label; it does not assert transport of every intracellular cargo. Proposed replacements: protein transport along microtubule Supporting Evidence: PMID:27462074 we found that cytoplasmic dynein is required for the spindle pole accumulation of Astrin, and dynein-mediated transport is important for balanced distribution of Astrin between spindle poles and kinetochores. |
| GO:0032388 positive regulation of intracellular transport | NAS PMID:33957056 Structure and Mechanics of Dynein Motors. | MODIFY | Summary: The review-level transport assertion can be expressed as microtubule-based transport. Reason: PMID:33957056 is a review rather than a new target experiment. Its broad positive-transport assertion agrees with direct human dynein motor reconstitution and transport studies. GO:0099111 describes the motor’s own transport work, rather than an unspecified upstream positive regulator; the review is retained as the original NAS source. Proposed replacements: microtubule-based transport Supporting Evidence: PMID:33957056 Cytoplasmic dynein is responsible for transporting intracellular cargos in interphase cells and mediating spindle assembly and chromosome positioning during cell division. |
| GO:0033962 P-body assembly | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Dynein-dependent stress-induced P-body growth is a supported contextual assembly role. Reason: The literal mouse Q9JHU4 donor carries IMP evidence from PMID:19825938. Its full Methods and Results show mouse DHC1 siRNA in NIH3T3 cells, with independent siRNA sets: stress-induced P-body growth is impaired while resting-body number/size is largely unchanged, and opposing-kinesin depletion reverses the effect. This supports motor-driven assembly under stress, not universal P-body formation or direct regulation of global translation. Retain the conserved contextual process. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: UniProtKB:Q9JHU4 SUPPORTS TRANSFER The mouse IMP source and its actual stress-dependent P-body phenotype support the transferred contextual process. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. |
| GO:0033962 P-body assembly | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Dynein-dependent stress-induced P-body growth is a supported contextual assembly role. Reason: The literal mouse Q9JHU4 donor carries IMP evidence from PMID:19825938. Its full Methods and Results show mouse DHC1 siRNA in NIH3T3 cells, with independent siRNA sets: stress-induced P-body growth is impaired while resting-body number/size is largely unchanged, and opposing-kinesin depletion reverses the effect. This supports motor-driven assembly under stress, not universal P-body formation or direct regulation of global translation. Retain the conserved contextual process. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: UniProtKB:Q9JHU4 SUPPORTS TRANSFER The mouse IMP source and its actual stress-dependent P-body phenotype support the transferred contextual process. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. |
| GO:0034063 cytoplasmic stress granule assembly | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Dynein contributes transport work to stress-granule assembly in the tested mammalian context. Reason: PMID:19825938 directly tests mouse DHC1 with independent siRNA sets and finds reduced stress-granule number/size after arsenite or thapsigargin; DHC2 does not substitute. Dynein-subunit localization and opposing-kinesin perturbation support an assembly/transport role downstream of eIF2-alpha phosphorylation. The literal Q9JHU4 IMP donor is preserved. Retain as contextual rather than a universal stress-response or translation-control mechanism. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: UniProtKB:Q9JHU4 SUPPORTS TRANSFER Actual mouse DHC1 perturbation supports stress-dependent granule assembly; source and target species remain distinct. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. |
| GO:0034063 cytoplasmic stress granule assembly | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Dynein contributes transport work to stress-granule assembly in the tested mammalian context. Reason: PMID:19825938 directly tests mouse DHC1 with independent siRNA sets and finds reduced stress-granule number/size after arsenite or thapsigargin; DHC2 does not substitute. Dynein-subunit localization and opposing-kinesin perturbation support an assembly/transport role downstream of eIF2-alpha phosphorylation. The literal Q9JHU4 IMP donor is preserved. Retain as contextual rather than a universal stress-response or translation-control mechanism. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: UniProtKB:Q9JHU4 SUPPORTS TRANSFER Actual mouse DHC1 perturbation supports stress-dependent granule assembly; source and target species remain distinct. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. |
| GO:0035578 azurophil granule lumen | TAS Reactome:R-HSA-6798751 | UNDECIDED | Summary: The curated azurophil-granule-lumen model membership remains unresolved at the target-assay level. Reason: The exact Reactome chain places human Q14204 residues 1–4646 in granule-lumen entity 6799155 within event R-HSA-6798751. The chain is authentic, but no target-specific neutrophil proteomic or intraluminal-localization record was recovered. Independent extracellular-vesicle detections do not resolve this narrower neutrophil organelle topology. Use UNDECIDED without claiming contamination, a secretion impossibility or a negative experiment. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasm, cytoskeleton file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0042802 identical protein binding | IDA PMID:28602352 Cryo-EM Reveals How Human Cytoplasmic Dynein Is Auto-inhibit... | ACCEPT | Summary: Human dynein heavy chains form the homodimeric motor. Reason: PMID:28602352 directly examines human dynein dimer conformations and engineered mutations in the autoinhibitory interface. This supports heavy-chain self-association. Preserve the paper’s literal mutation-label discrepancies in the evidence record rather than inventing a harmonized sequence; dimerization does not become a separate mechanistic core from the motor assembly. Supporting Evidence: PMID:28602352 The heavy chain (HC) N-terminal dimerization domain (NDD) holds HC A and HC B together. file:human/DYNC1H1/DYNC1H1-uniprot.txt Homodimer. |
| GO:0045505 dynein intermediate chain binding | IBA GO_REF:0000033 | ACCEPT | Summary: Intermediate-chain binding is a conserved heavy-chain assembly activity. Reason: The literal PTN000743491 IBD for intermediate-chain binding is ancestral to the target. Its placement is compatible with the conserved heavy-chain tail architecture and human multicomponent dynein assembly. The source’s Dictyostelium descendant is a legitimate grounding of the ancestral placement, not weak evidence merely because only one donor is listed. No purified human two-protein affinity is claimed from that donor. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000743491 SUPPORTS TRANSFER The exact IBD row and human target descent were independently reconstructed from the current PTHR10676 tree/MSA and literal PAINT source. No target-specific functional loss was established; donor count and target self-grounding are not failure criteria. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt The ICs and LICs bind directly to the HC dimer and dynein LCs assemble on the IC dimer file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0045505 dynein intermediate chain binding | IEA GO_REF:0000002 | ACCEPT | Summary: The intermediate-chain binding mapping agrees with conserved dynein assembly. Reason: The IPR026983-based source is consistent with the DYNC1H1 tail and its intermediate-chain association in human dynein complexes. Keep binding as an assembly mechanism within the motor, without attributing every complex-level interaction to a separately purified heavy-chain interface. Propagation Review Root cause: NO FAILURE CORE Sources checked: InterPro:IPR026983 SUPPORTS TRANSFER The imported heavy-chain family mapping agrees with direct target complex-assembly evidence. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt The ICs and LICs bind directly to the HC dimer and dynein LCs assemble on the IC dimer |
| GO:0051293 establishment of spindle localization | IMP PMID:23027904 Cortical dynein is critical for proper spindle positioning i... | MODIFY | Summary: The directly demonstrated positioning process is establishment of mitotic spindle localization. Reason: PMID:23027904 uses DYNC1H1 siRNA in human HeLa cells and tests cortical G-alpha-i/LGN/NuMA-dependent dynein recruitment. Heavy-chain depletion randomizes spindle position, and cortical recruitment directs positioning. GO:0040001 specifies the mitotic process tested and is a typed descendant of broad establishment of spindle localization. The assay is not generalized to all meiotic or other spindle contexts. Proposed replacements: establishment of mitotic spindle localization Supporting Evidence: PMID:23027904 or of the dynein heavy chain DYNC1H1 results in the spindle adopting a significantly more random position PMID:23027904 we demonstrated that the mere presence of dynein at that location is sufficient to direct spindle positioning in HeLa cells. |
| GO:0051959 dynein light intermediate chain binding | IBA GO_REF:0000033 | ACCEPT | Summary: Light-intermediate-chain binding is a conserved assembly activity with human corroboration. Reason: The literal PTN000743491 IBD for GO:0051959 reaches the target and includes experimentally annotated descendants, including DYNC1H1 itself. That self-grounding is legitimate PAINT evidence, not circularity. Human LIC-domain coimmunoprecipitation/mutagenesis in PMID:25272277 independently supports the heavy-chain association. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000743491 SUPPORTS TRANSFER The exact IBD row and human target descent were independently reconstructed from the current PTHR10676 tree/MSA and literal PAINT source. No target-specific functional loss was established; donor count and target self-grounding are not failure criteria. Supporting Evidence: PMID:25272277 We show that the LIC G domain binds the dynein heavy chain using a conserved patch of aromatic residues file:human/DYNC1H1/DYNC1H1-uniprot.txt DYNC1LI1 and DYNC1LI2 bind mutually exclusive to DYNC1H1 file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0051959 dynein light intermediate chain binding | IEA GO_REF:0000120 | ACCEPT | Summary: The combined orthology/domain mapping supports light-intermediate-chain binding. Reason: Both the literal mouse Q9JHU4 source and IPR026983 are preserved. The current donor record carries the LIC-binding assertion, and PMID:25272277 directly maps human LIC G-domain association with heavy chain. Preserve the distinction between cellular domain mapping and purified binary binding; no loss or incompatible heavy-chain architecture is established. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:Q9JHU4 SUPPORTS TRANSFER The mouse source is retained together with the imported InterPro mapping; direct human LIC-domain association independently supports the activity. InterPro:IPR026983 SUPPORTS TRANSFER The heavy-chain family mapping is compatible with the experimentally supported human LIC interaction. Supporting Evidence: PMID:25272277 We show that the LIC G domain binds the dynein heavy chain using a conserved patch of aromatic residues file:human/DYNC1H1/DYNC1H1-uniprot.txt DYNC1LI1 and DYNC1LI2 bind mutually exclusive to DYNC1H1 |
| GO:0051959 dynein light intermediate chain binding | IPI PMID:25272277 A Ras-like domain in the light intermediate chain bridges th... | ACCEPT | Summary: Human LIC1 association with heavy chain is mapped experimentally. Reason: PMID:25272277 reports heavy-chain association of human DYNC1LI1/Q9Y6G9 and its 1–389 G-domain construct, with loss of the same association for the C-terminal tail alone and effects of conserved-surface mutations. This supports the retained LIC-binding activity; the cellular coimmunoprecipitation is not described as a purified affinity assay. Supporting Evidence: PMID:25272277 We show that the LIC G domain binds the dynein heavy chain using a conserved patch of aromatic residues file:human/DYNC1H1/DYNC1H1-uniprot.txt DYNC1LI1 and DYNC1LI2 bind mutually exclusive to DYNC1H1 |
| GO:0060236 regulation of mitotic spindle organization | IMP PMID:23027904 Cortical dynein is critical for proper spindle positioning i... | MODIFY | Summary: The broad spindle-regulation assertion is refined to the positioning work directly tested. Reason: The human HeLa perturbation/recruitment experiments in PMID:23027904 demonstrate directed mitotic spindle positioning by cortical dynein. GO:0040001 captures that work more directly than generic regulation of spindle organization. The source row remains distinct from the other annotation from this paper; neither is converted into a NEW assertion. Proposed replacements: establishment of mitotic spindle localization Supporting Evidence: PMID:23027904 or of the dynein heavy chain DYNC1H1 results in the spindle adopting a significantly more random position PMID:23027904 we demonstrated that the mere presence of dynein at that location is sufficient to direct spindle positioning in HeLa cells. |
| GO:0070062 extracellular exosome | HDA PMID:19056867 Large-scale proteomics and phosphoproteomics of urinary exos... | KEEP AS NON CORE | Summary: Human urinary-exosome proteomics positively detects DYNC1H1. Reason: The authors’ NHLBI database linked from PMID:19056867 explicitly lists DYNC1H1/NP_001367 with 14 peptides and reference 2, the Gonzales study. Original Methods describe a low-density urinary membrane fraction chiefly containing exosomes, target-decoy filtering and peptide-disambiguation checks. Retain contextual fraction detection without claiming a target-specific intravesicular topology image, vesicle-biogenesis function or a new secretion mechanism. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasm, cytoskeleton file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md |
| GO:0070062 extracellular exosome | HDA PMID:19199708 Proteomic analysis of human parotid gland exosomes by multid... | KEEP AS NON CORE | Summary: Human parotid-exosome proteomics positively detects DYNC1H1. Reason: The genuine PMID:19199708 supplementary Table 2 lists IPI00456969.1/DYNC1H1 in the Paratid Exosomes sheet, physical row 433, with 5 sequences, 9 spectra and 2.1% coverage of the 4646-residue protein. The original samples parotid-duct saliva from one healthy donor and uses a differential-centrifugation vesicle preparation with general microscopy/marker controls. This supports a contextual preparation-level location, not single-vesicle localization or a function in vesicle production. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasm, cytoskeleton file:human/DYNC1H1/DYNC1H1-evidence/RESULTS.md file:human/DYNC1H1/DYNC1H1-source-evidence.md |
| GO:0070062 extracellular exosome | HDA PMID:20458337 MHC class II-associated proteins in B-cell exosomes and pote... | KEEP AS NON CORE | Summary: The curated exosome assertion is retained with a source-specific target-table limit. Reason: PMID:20458337 is represented by its authentic abstract, but the target-specific full proteomic evidence was not recovered. Independent human urinary and parotid exosome-associated detections in PMID:19056867 and PMID:19199708 support this same broad contextual location. Retain the curator’s positive source assertion without claiming that its own DYNC1H1 peptides were inspected or that all extracellular preparations are equivalent. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasm, cytoskeleton |
| GO:0070062 extracellular exosome | HDA PMID:21362503 Protein profile of exosomes from trabecular meshwork cells. | KEEP AS NON CORE | Summary: The exosome-associated location is retained with the trabecular-meshwork assay scope explicit. Reason: The actual PMID:21362503 Methods describe vesicle preparations from cultured human trabecular-meshwork cells, with differential centrifugation/washing and proteomic filtering. Its exact target supplementary table was not recovered; do not claim a verified DYNC1H1 peptide row or substitute urinary samples for the cultured-cell source. Independent human exosome-associated detections support the broad location, which remains contextual rather than a vesicle function. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasm, cytoskeleton file:human/DYNC1H1/DYNC1H1-source-evidence.md |
| GO:0070062 extracellular exosome | HDA PMID:23533145 In-depth proteomic analyses of exosomes isolated from expres... | KEEP AS NON CORE | Summary: The exosome-associated location is retained without overstating the prostate-fluid source table. Reason: PMID:23533145 profiles pooled expressed-prostatic-secretion urine vesicle preparations with defined centrifugation, washing and proteomic thresholds. The exact DYNC1H1 supplementary entry remains uninspected. Retain the curated broad location in light of independently verified human exosome-associated detection, while preserving this source’s sample and access limits. A protein detected in a preparation is not thereby a vesicle-biogenesis factor. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Cytoplasm, cytoskeleton |
| GO:0090235 regulation of metaphase plate congression | IMP PMID:27462074 Nuclear Mitotic Apparatus (NuMA) Interacts with and Regulate... | KEEP AS NON CORE | Summary: The human spindle-pole transport phenotype supports contextual regulation of chromosome congression. Reason: PMID:27462074 shows that DYNC1H1 depletion impairs pole accumulation of NuMA/Astrin and is accompanied by chromosome-alignment/congression defects in human HeLa cells. This is a positive cell-division contribution through motor-dependent spindle organization, not merely a disease association. Retain the regulatory process as contextual; a direct heavy-chain–chromosome recognition mechanism is not inferred. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Plays a role in mitotic spindle assembly and metaphase plate congression PMID:27462074 we found that cytoplasmic dynein is required for the spindle pole accumulation of Astrin, and dynein-mediated transport is important for balanced distribution of Astrin between spindle poles and kinetochores. |
| GO:0090267 positive regulation of mitotic cell cycle spindle assembly checkpoint | NAS PMID:19229290 Dynein light intermediate chain 1 is required for progress t... | MODIFY | Summary: The cited checkpoint mechanism is deactivation, not positive maintenance of the checkpoint. Reason: The authentic PMID:19229290 abstract and publisher preview explicitly describe LIC1-dependent removal of Mad1/2 and Zw10 from kinetochores during checkpoint silencing. The original source is NAS for the heavy chain; full target-specific Methods were not recovered, and the LIC1 perturbation is not relabeled as a DYNC1H1-specific assay. GO:1902426 expresses the supported dynein-complex transport/deactivation mechanism more accurately than positive regulation of checkpoint activity. Proposed replacements: deactivation of mitotic spindle assembly checkpoint Supporting Evidence: PMID:19229290 Cytoplasmic dynein functions in the checkpoint, apparently by moving critical checkpoint components off kinetochores. PMID:19229290 We conclude that a Cdk1 phosphorylated form of LIC1 is required to remove Mad1/2 and Zw10 but not BubR1 from kinetochores during spindle assembly checkpoint silencing. |
| GO:0120162 positive regulation of cold-induced thermogenesis | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Mouse dynein perturbation supports a contextual positive role in cold-induced thermogenesis. Reason: The literal Q9JHU4 mouse source carries IMP evidence from PMID:20887786. The authentic abstract reports cold intolerance in dynein-mutant mice and links impaired norepinephrine-stimulated adipocyte responses to oxidative stress; it does not establish a separate human thermogenic enzyme. GO:0120162 includes increasing the rate or extent of cold-induced thermogenesis, so a supported regulatory effect is not contradicted merely because its mechanism is indirect. Retain the conserved contextual process while acknowledging that detailed full-text assay reconstruction remains unavailable. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: UniProtKB:Q9JHU4 SUPPORTS TRANSFER The current mouse IMP assertion and authentic cold-response phenotype support a contextual regulatory transfer, not direct human thermogenic chemistry. Supporting Evidence: PMID:20887786 our results provide genetic evidence that dynein plays a key role in lipid metabolism and thermogenesis through a modulation of oxidative stress elicited by norepinephrine. |
| GO:0120162 positive regulation of cold-induced thermogenesis | ISS PMID:20887786 Mutations in cytoplasmic dynein lead to a Huntington's disea... | KEEP AS NON CORE | Summary: Mouse dynein perturbation supports a contextual positive role in cold-induced thermogenesis. Reason: The literal Q9JHU4 mouse source carries IMP evidence from PMID:20887786. The authentic abstract reports cold intolerance in dynein-mutant mice and links impaired norepinephrine-stimulated adipocyte responses to oxidative stress; it does not establish a separate human thermogenic enzyme. GO:0120162 includes increasing the rate or extent of cold-induced thermogenesis, so a supported regulatory effect is not contradicted merely because its mechanism is indirect. Retain the conserved contextual process while acknowledging that detailed full-text assay reconstruction remains unavailable. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: UniProtKB:Q9JHU4 SUPPORTS TRANSFER The current mouse IMP assertion and authentic cold-response phenotype support a contextual regulatory transfer, not direct human thermogenic chemistry. Supporting Evidence: PMID:20887786 our results provide genetic evidence that dynein plays a key role in lipid metabolism and thermogenesis through a modulation of oxidative stress elicited by norepinephrine. |
| GO:1904115 axon cytoplasm | IEA GO_REF:0000108 | ACCEPT | Summary: Axonal cytoplasm is a functional compartment of retrograde dynein transport. Reason: The electronic logical inference derives the location from the target’s retrograde-axonal-transport annotation. The literal PAINT axonal-transport node and human motor architecture support this functional pool. This does not imply that DYNC1H1 is neuron-specific or excludes its mitotic/cytoplasmic pools. Propagation Review Root cause: NO FAILURE CORE Sources checked: GO:0008090 SUPPORTS TRANSFER The imported logical rule links retrograde axonal transport to its axonal compartment; the underlying transport assertion is retained. Supporting Evidence: PMID:21820100 Cytoplasmic dynein is a large multisubunit motor protein complex and has a key role in retrograde axonal transport in neurons. |
| GO:1905832 positive regulation of spindle assembly | IMP PMID:27462074 Nuclear Mitotic Apparatus (NuMA) Interacts with and Regulate... | KEEP AS NON CORE | Summary: The human dynein perturbation supports a contextual positive contribution to mitotic spindle assembly. Reason: PMID:27462074 directly depletes DYNC1H1 in human HeLa cells and measures disrupted spindle-pole cargo accumulation and spindle organization. The heavy chain supplies transport/force within assembly rather than being only an expression marker. Retain this regulatory process as contextual alongside the more direct motor/organization synthesis; no separate assembly catalyst is invented. Supporting Evidence: file:human/DYNC1H1/DYNC1H1-uniprot.txt Plays a role in mitotic spindle assembly and metaphase plate congression |
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Download this section (compressed HTML)Q: Which DYNC1H1 variants selectively alter motor activation, cargo-adaptor assembly and neuronal nuclear movement, rather than causing a uniform reduction of all dynein-dependent transport?
Q: Does the curated mouse filopodium observation reflect a reproducible human DYNC1H1 pool, and which target-specific assay supports the Reactome azurophil-granule lumen placement?
Q: What RNAs contact the heavy chain in UV-capture experiments, and do these contacts contribute to cargo transport or another regulated cellular role?
Experiment: Compare matched human DYNC1H1 variants in reconstituted dynein–dynactin–adaptor motility assays and human neuronal cargo/nuclear-movement imaging, with controls for motor-complex abundance, assembly and adaptor recruitment.
Experiment: Test the reported extracellular-vesicle and narrow compartment pools with target-specific imaging, quantitative purification controls and protease-protection assays, distinguishing particle association from lumenal cargo and unrelated co-isolation.
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