DYNC2I2 (WDR34) is a WD-repeat intermediate chain of cytoplasmic dynein-2, the motor that returns intraflagellar transport components and cargo toward the ciliary base. It supplies light-chain, intermediate-chain and heavy-chain contacts that organize the asymmetric motor complex. Its ciliary transport and assembly roles involve basal-body, ciliary-interior and tip-turnaround pools; the severity of ciliary defects varies with the disrupted allele or engineered fragment. Biallelic variants cause skeletal ciliopathies. In human HEK293 cells, WDR34 also associates with TAK1/TAB2/TRAF6 and attenuates IL-1/TLR signaling, a reported nonciliary activity whose relationship to dynein-2 remains unresolved.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0001673 male germ cell nucleus | NAS PMID:36973253 Distinct dynein complexes defined by DYNLRB1 and DYNLRB2 reg... | UNDECIDED | Summary: The positive Complex Portal assertion is preserved, but the human male-germ-cell nuclear location remains unresolved. Reason: The positive Complex Portal assertion is preserved, but the human male-germ-cell nuclear location remains unresolved. Current dynein-2 variants CPX-26363βCPX-26366 carry this location. The original mouse study does contain Wdr34: Supplementary Figure 2b reports 31 Wdr34 clones in a DYNLRB2 yeast two-hybrid screen of mouse testis cDNA. That corrects the prior claim that the paper does not mention WDR34. The main nuclear-envelope experiments use mouse DYNLRB2 and dynein-1 intermediate-chain reagents; the Wdr34 screen hit does not itself localize human DYNC2I2 inside germ-cell nuclei. Neither this assay nor uncertain nuclear staining in unrelated HPA cell lines resolves the imported compartment claim. Supporting Evidence: PMID:36973253 Dynein accumulates on the cytoplasmic side of the telomere-nuclear envelope attachment sites and drives the movement of the chromosomes file:human/DYNC2I2/DYNC2I2-source-guide.md |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | KEEP AS NON CORE | Summary: The human WDR34βcoilin pair is positively recorded by array, prey-pooling and validated two-hybrid assays (including IM-23318-40342). Reason: The human WDR34βcoilin pair is positively recorded by array, prey-pooling and validated two-hybrid assays (including IM-23318-40342). The array/pooling records assign residues 118β536 to WDR34, not to coilin. Both reference sequences and taxon identifiers are authenticated. Coilin is a Cajal-body component, but no informative binding subclass was established for this pair. Retain the supported association as non-core rather than reject it for lacking physiological context; it does not establish a nuclear-localization or RNA-processing function for DYNC2I2. The CLINGEN_MENDELIAN project explicitly retains supported generic binding as non-core when no informative replacement is established; its task-specific instruction applies to this re-review. Supporting Evidence: file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MODIFY | Summary: Three positive human WDR34βTRIM54 records include validated two-hybrid IM-23318-41698; array/pooling records specify WDR34 residues 118β536. Reason: Three positive human WDR34βTRIM54 records include validated two-hybrid IM-23318-41698; array/pooling records specify WDR34 residues 118β536. Reference-sequence ROGIDs authenticate both literal participants. Current human TRIM54 annotation classifies the partner as an E3 ubiquitin-protein ligase (GO:0061630, curated IBA), supporting the informative ubiquitin-protein-ligase-binding class. This refines the identity of the bound partner; it does not assert that WDR34 is an enzyme, a ligase substrate, or a regulator of ubiquitination. The partner-class evidence is propagated functional curation, not a new direct enzymatic assay in the interaction screen. GO:0031625 describes binding to an E3 protein. The positive WDR34 interaction supplies the binding evidence, and independent TRIM54 curation supplies the class of the bound object; neither step transfers ligase catalysis to WDR34. Proposed replacements: ubiquitin protein ligase binding Supporting Evidence: file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:27173435 An organelle-specific protein landscape identifies novel dis... | MODIFY | Summary: The literal pair is WDR34 and WDR60/DYNC2I1. Reason: The literal pair is WDR34 and WDR60/DYNC2I1. IntAct records IM-25054-190 and IM-25054-192 are reciprocal tandem-affinity-purification observations with spoke expansion in the ciliary interaction network. They establish complex association rather than purified binary affinity. WDR60 is an independently established dynein intermediate chain, so dynein intermediate chain binding is an informative refinement without changing the assay scope. Proposed replacements: dynein intermediate chain binding Supporting Evidence: PMID:31451806 dynein-2 heavy chain are contorted into different conformations by a WDR60-WDR34 heterodimer file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:30649997 Interactions of the dynein-2 intermediate chain WDR34 with t... | MODIFY | Summary: VIP and immunoblot assays map association of WDR34 with the LC8-type light chain DYNLL2 to residues 80β93. Reason: VIP and immunoblot assays map association of WDR34 with the LC8-type light chain DYNLL2 to residues 80β93. Deleting this site prevents rescue of retrograde-trafficking defects in WDR34-knockout RPE1 cells. Refine to dynein light chain binding. The binding measurements use fluorescent fusion proteins in HEK293T lysates; they are not purified affinity measurements, and the unavailable supplementary construct table prevents an independently sequence-authenticated assignment of every introduced insert. Proposed replacements: dynein light chain binding Supporting Evidence: PMID:30649997 By narrowing down the region of WDR34, we found that the region encompassing residues 80β93 is important for the interaction of WDR34 with DYNLL2 file:human/DYNC2I2/DYNC2I2-source-guide.md |
| GO:0005515 protein binding | IPI PMID:30649997 Interactions of the dynein-2 intermediate chain WDR34 with t... | MODIFY | Summary: The original mapped WDR34 association with the roadblock light chain DYNLRB1 to the 106β131 region, distinct from the DYNLL2 site. Reason: The original mapped WDR34 association with the roadblock light chain DYNLRB1 to the 106β131 region, distinct from the DYNLL2 site. The tested deletion of residues 116β131 failed to rescue ciliary IFT88/GPR161 retention. Refine this literal Q9NP97 partner to dynein light chain binding. Preserve the cellular extract assay and supplementary-construct access limits; no autonomous motor activity or natural isoform-specific loss is inferred. Proposed replacements: dynein light chain binding Supporting Evidence: PMID:30649997 In this study, we demonstrated that the WDR34 intermediate chain interacts with the two light chains, DYNLL1/DYNLL2 and DYNLRB1/DYNLRB2, via its distinct sites. file:human/DYNC2I2/DYNC2I2-source-guide.md |
| GO:0005515 protein binding | IPI PMID:31451806 Structure of the dynein-2 complex and its assembly with intr... | MODIFY | Summary: The recombinant human dynein-2 structure establishes the asymmetric WDR34βWDR60 intermediate-chain assembly. Reason: The recombinant human dynein-2 structure establishes the asymmetric WDR34βWDR60 intermediate-chain assembly. This directly supports the dynein intermediate chain binding refinement for the original Q8WVS4 partner. The motor experiments concern the assembled multisubunit complex and deletion of the intermediate/light-chain block, not isolated WDR34 ATPase activity or a WDR34-only knockout. Proposed replacements: dynein intermediate chain binding Supporting Evidence: PMID:31451806 dynein-2 heavy chain are contorted into different conformations by a WDR60-WDR34 heterodimer file:human/DYNC2I2/DYNC2I2-source-guide.md |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: The literal partner Q6FHY5 is a human MEOX2 record, not an unidentified protein. Reason: The literal partner Q6FHY5 is a human MEOX2 record, not an unidentified protein. Three positive HuRI two-hybrid records include IM-25472-117217 and assign the WDR34 binding region to residues 118β536. ROGIDs match the referenced proteins. Q6FHY5 is 303 aa; comparison with the 304-aa reviewed MEOX2 P50222 has one insertion outside the conserved homeobox; its complete DNA-binding region is identical. MEOX2 is a DNA-binding transcription factor, supporting this partner-class refinement without asserting that WDR34 regulates a MEOX2 target gene or that the assay demonstrated a native transcriptional complex. GO:0140297 describes binding to a DNA-binding transcription factor, not transcription-factor activity in the binding protein. The positive interaction and independently established MEOX2 class are the two supporting observations. Proposed replacements: DNA-binding transcription factor binding Supporting Evidence: file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | MODIFY | Summary: BioPlex records IM-29278-17087 and IM-29278-23459 identify tagged WDR60/DYNC2I1 as bait and WDR34 as prey; one record explicitly uses spoke expansion. Reason: BioPlex records IM-29278-17087 and IM-29278-23459 identify tagged WDR60/DYNC2I1 as bait and WDR34 as prey; one record explicitly uses spoke expansion. The positive co-purification and verified intermediate-chain identity support dynein intermediate chain binding. The screen does not independently establish a binary interface or a new molecular mechanism, and the literal source partner remains Q8WVS4. Proposed replacements: dynein intermediate chain binding Supporting Evidence: file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0005515 protein binding | IPI PMID:40205054 Multimodal cell maps as a foundation for structural and func... | MODIFY | Summary: The original AP-MS network contains the DYNC2I1-bait to DYNC2I2-prey edge 556070, with bait antibody HPA020607. Reason: The original AP-MS network contains the DYNC2I1-bait to DYNC2I2-prey edge 556070, with bait antibody HPA020607. This is the measured AP-MS graph, not a predicted embedding. The independently established intermediate-chain identity of the bait supports dynein intermediate chain binding. Association in an isolated complex is preserved as such; this record is not a purified direct-contact experiment. Proposed replacements: dynein intermediate chain binding Supporting Evidence: file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0005737 cytoplasm | EXP PMID:19521662 WDR34 is a novel TAK1-associated suppressor of the IL-1R/TLR... | KEEP AS NON CORE | Summary: The human HEK293 study reports cytoplasmic WDR34 and endogenous association with TAK1/TAB2/TRAF6, together with stimulus-dependent reporter and phosphorylation effects. Reason: The human HEK293 study reports cytoplasmic WDR34 and endogenous association with TAK1/TAB2/TRAF6, together with stimulus-dependent reporter and phosphorylation effects. Retain this positive nonciliary pool as contextual. The proposed sequestration mechanism is a model, and RNAi-3/RNAi-8 have the same published target sequence. Cytoplasm is not being rejected as incompatible with the ciliary motor role. Supporting Evidence: PMID:19521662 Immunofluorescence experiments showed that WDR34 was mainly localized in the cytoplasm file:human/DYNC2I2/DYNC2I2-source-guide.md |
| GO:0005737 cytoplasm | IDA PMID:25205765 Subunit composition of the human cytoplasmic dynein-2 comple... | KEEP AS NON CORE | Summary: The study distinguishes endogenous pericentrosomal staining from human mGFP-WDR34 in RPE1 cells, which also has a diffuse cytoplasmic pool. Reason: The study distinguishes endogenous pericentrosomal staining from human mGFP-WDR34 in RPE1 cells, which also has a diffuse cytoplasmic pool. The authors note that proteolytic release of GFP may contribute to some diffuse signal. Retain the broad positive cytoplasmic assertion without treating all fluorescence as soluble native WDR34 or restricting the gene exclusively to cilia. Supporting Evidence: PMID:25205765 mGFPβWDR34 localizes to centrosomes and primary cilia in serum-starved cells as well as showing a diffuse cytoplasmic distribution. file:human/DYNC2I2/DYNC2I2-source-guide.md |
| GO:0005737 cytoplasm | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: The UniProt subcellular-location mapping and current ARBA00026971 rule support a broad cytoplasmic pool. Reason: The UniProt subcellular-location mapping and current ARBA00026971 rule support a broad cytoplasmic pool. The target matches the two WDR34 FunFam conditions in the rule, and independent human studies support nonciliary cytoplasmic/pericentrosomal distribution. Retain this general contextual location; it does not specify a membrane, a ciliary subcompartment, or a distinct autonomous activity. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: ARBA:ARBA00026971 SUPPORTS TRANSFER UniProtKB-SubCell:SL-0086 SUPPORTS TRANSFER Supporting Evidence: file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0005813 centrosome | IDA GO_REF:0000052 | KEEP AS NON CORE | Summary: The actual HPA target page assigns supported reliability to centrosome for antibody HPA040764 in several human cell lines. Reason: The actual HPA target page assigns supported reliability to centrosome for antibody HPA040764 in several human cell lines. Basal-body and cytosolic observations are separately supported, whereas its nuclear locations are uncertain. Retain the centrosomal pool as contextual without reducing every centrosome to a basal body or transferring aggregate reliability to each location. Supporting Evidence: file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0005813 centrosome | IDA PMID:25205765 Subunit composition of the human cytoplasmic dynein-2 comple... | KEEP AS NON CORE | Summary: Endogenous WDR34 immunofluorescence in human RPE1 cells concentrates around centrosomes, including a pool outside detectable cilia. Reason: Endogenous WDR34 immunofluorescence in human RPE1 cells concentrates around centrosomes, including a pool outside detectable cilia. This source supports centrosome at its measured resolution; the functional basal-body/ciliary pools are independently represented. Retain the broader centrosomal observation without claiming exclusive localization. Supporting Evidence: PMID:25205765 mGFPβWDR34 localizes to centrosomes and primary cilia in serum-starved cells file:human/DYNC2I2/DYNC2I2-source-guide.md |
| GO:0005813 centrosome | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: SL-0048 is the UniProt centrosome-location mapping. Reason: SL-0048 is the UniProt centrosome-location mapping. It is concordant with endogenous human pericentrosomal staining and supported HPA centrosome observations. Retain this broader contextual pool alongside the separately supported basal-body location; centrosome is not synonymous with basal body. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: UniProtKB-SubCell:SL-0048 SUPPORTS TRANSFER Supporting Evidence: file:human/DYNC2I2/DYNC2I2-source-guide.md |
| GO:0005814 centriole | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: The literal Ensembl/UniProt donor is mouse Wdr34 Q5U4F6. Reason: The literal Ensembl/UniProt donor is mouse Wdr34 Q5U4F6. Its primary localization evidence includes centrioles and surrounding puncta in mouse cells, while human fibroblasts show a related basal-body pool. Retain the ortholog-supported centriole observation as contextual. It does not establish that only the cilium-bearing mother centriole is labeled, so replacing the complete claim with basal body would discard source scope. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: UniProtKB:Q5U4F6 SUPPORTS TRANSFER |
| GO:0005814 centriole | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Mouse Q5U4F6 is the verified Wdr34 donor, with direct centriole/localization evidence in PMID:24183451. Reason: Mouse Q5U4F6 is the verified Wdr34 donor, with direct centriole/localization evidence in PMID:24183451. The human gene is in the same WDR34 family branch. Retain the broader centriole assertion as contextual rather than equate all observed centrioles with basal bodies or treat ortholog evidence as direct human imaging. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: UniProtKB:Q5U4F6 SUPPORTS TRANSFER |
| GO:0005829 cytosol | IDA GO_REF:0000052 | KEEP AS NON CORE | Summary: HPA assigns supported reliability specifically to cytosol in its human DYNC2I2 records; this is not inferred from an overall reliability score. Reason: HPA assigns supported reliability specifically to cytosol in its human DYNC2I2 records; this is not inferred from an overall reliability score. Retain the nonciliary cytosolic pool. The observation does not itself define where motor assembly or the reported TAK1-related effects occur, and it is not evidence against the ciliary pool. Supporting Evidence: file:human/DYNC2I2/DYNC2I2-source-guide.md |
| GO:0005868 cytoplasmic dynein complex | IBA GO_REF:0000033 | ACCEPT | Summary: The target PTN002488185 lies below the WDR34-clade node PTN000278083 in the current PTHR12442 tree, and the literal IBD carries cytoplasmic dynein complex. Reason: The target PTN002488185 lies below the WDR34-clade node PTN000278083 in the current PTHR12442 tree, and the literal IBD carries cytoplasmic dynein complex. Its human self-donor is legitimate experimental grounding, not circularity; mouse Q5U4F6 provides ortholog support. GO:0005868 explicitly includes intraflagellar dyneins and is named phylogenetically, so its name does not restrict DYNC2I2 to nonciliary cytoplasm. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000278083 SUPPORTS TRANSFER Literal current IBD and target descendant path verified in the saved PTHR12442 tree; target alignment sequence exactly matches official Q96EX3. Donor current records and source-specific limitations are preserved separately. Supporting Evidence: PMID:25205765 We show that the proteins encoded by the ciliopathy genes WDR34 and WDR60 are bona fide dynein-2 intermediate chains and are both required for dynein-2 function. file:human/DYNC2I2/DYNC2I2-deep-research-falcon.md It helps assemble and organize the motor, anchors one DYNC2H1 heavy chain, recruits light chains, and supports transport of intraflagellar-transport (IFT) machinery within cilia. file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0005868 cytoplasmic dynein complex | IDA PMID:25205765 Subunit composition of the human cytoplasmic dynein-2 comple... | ACCEPT | Summary: Human WDR34 affinity purification and immunoblot validation identify the dynein-2 subunit assembly in RPE1 cells. Reason: Human WDR34 affinity purification and immunoblot validation identify the dynein-2 subunit assembly in RPE1 cells. This supports membership in GO:0005868, whose definition includes the intraflagellar dynein motor. The cellular purification is complex evidence, not a measurement of autonomous WDR34 motor activity. Supporting Evidence: PMID:25205765 The above data indicate that both WDR34 and WDR60 are intermediate chains of the dynein-2 complex. file:human/DYNC2I2/DYNC2I2-source-guide.md |
| GO:0005868 cytoplasmic dynein complex | IDA PMID:25830415 Tctex1d2 associates with short-rib polydactyly syndrome prot... | ACCEPT | Summary: The source shows endogenous human association among TCTEX1D2, WDR34 and WDR60 and complementary cell-free interaction assays. Reason: The source shows endogenous human association among TCTEX1D2, WDR34 and WDR60 and complementary cell-free interaction assays. These establish WDR34 as a dynein-2 component. The complex classification is appropriate even though the paper also studies patient variants and nonhuman model systems; those contexts are not substituted for the human biochemical evidence. Supporting Evidence: PMID:25830415 Tctex1d2 IPd Dync2h1, Wdr60, Wdr34, Dync2li1 and Dynll1 file:human/DYNC2I2/DYNC2I2-source-guide.md |
| GO:0005868 cytoplasmic dynein complex | IDA PMID:31451806 Structure of the dynein-2 complex and its assembly with intr... | ACCEPT | Summary: Reconstituted human dynein-2 contains WDR34 as one of its two asymmetric intermediate chains. Reason: Reconstituted human dynein-2 contains WDR34 as one of its two asymmetric intermediate chains. The structure and intact-complex assays support cytoplasmic dynein complex membership. The term explicitly accommodates ciliary dynein-2; it is neither an axonemal dynein arm assignment nor an autonomous ATPase annotation. Supporting Evidence: PMID:31451806 DHC2TAIL-A and -B, are bound by the Ξ²-propeller of WDR60 and WDR34 respectively, mainly via bundle 3 file:human/DYNC2I2/DYNC2I2-source-guide.md |
| GO:0005868 cytoplasmic dynein complex | IEA GO_REF:0000120 | ACCEPT | Summary: Current ARBA00088183 assigns GO:0005868 to the target-matched WDR34 FunFam pair within Eukaryota; the combined source also includes verified mouse Q5U4F6. Reason: Current ARBA00088183 assigns GO:0005868 to the target-matched WDR34 FunFam pair within Eukaryota; the combined source also includes verified mouse Q5U4F6. Independent human biochemical and structural evidence confirms the transferred complex membership. No target-specific loss or paralog mismatch is indicated. Propagation Review Root cause: NO FAILURE CORE Sources checked: ARBA:ARBA00088183 SUPPORTS TRANSFER UniProtKB:Q5U4F6 SUPPORTS TRANSFER Supporting Evidence: file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0005929 cilium | IDA PMID:25205765 Subunit composition of the human cytoplasmic dynein-2 comple... | MODIFY | Summary: Human mGFP-WDR34 is observed along RPE1 cilia, and the protein is a nonmembrane dynein-2 subunit. Reason: Human mGFP-WDR34 is observed along RPE1 cilia, and the protein is a nonmembrane dynein-2 subunit. Refine the broad cilium assertion to ciliary plasm, the ciliary contents excluding the surrounding membrane. This integrated refinement retains the observed ciliary location without claiming that the fluorescence resolves a precise microtubule-binding site; the basal-body pool is represented separately. Proposed replacements: ciliary plasm Supporting Evidence: PMID:25205765 (A) mGFPβWDR34 (green) localizes to a cytosolic pool and to primary cilia, colocalizing with acetylated tubulin (red). file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0005929 cilium | IEA GO_REF:0000120 | MODIFY | Summary: The verified mouse Wdr34 donor and UniProt cilium mapping support the ciliary pool, and human biochemical/localization evidence places WDR34 in the nonmembrane dynein-2 machinery. Reason: The verified mouse Wdr34 donor and UniProt cilium mapping support the ciliary pool, and human biochemical/localization evidence places WDR34 in the nonmembrane dynein-2 machinery. Refine to ciliary plasm, a current part_of descendant of cilium. The ortholog and location-map sources are retained, and the refinement does not turn the transported dynein-2 complex into a ciliary membrane protein. Propagation Review Root cause: TERM SCOPING PROBLEM Failure modes: GRANULARITY MISMATCH Sources checked: UniProtKB:Q5U4F6 SUPPORTS TRANSFER UniProtKB-SubCell:SL-0066 SUPPORTS TRANSFER Proposed replacements: ciliary plasm Supporting Evidence: file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0005929 cilium | NAS PMID:30065109 Emerging mechanisms of dynein transport in the cytoplasm ver... | MODIFY | Summary: The source review explicitly distinguishes dynein-2 from axonemal beating motors and identifies WDR34/FAP133 as an intermediate-chain subunit of the intraflagellar motor. Reason: The source review explicitly distinguishes dynein-2 from axonemal beating motors and identifies WDR34/FAP133 as an intermediate-chain subunit of the intraflagellar motor. Refine its broad cilium statement to ciliary plasm using the established nonmembrane complex identity; this remains a secondary-source statement, not a new imaging experiment. Proposed replacements: ciliary plasm Supporting Evidence: file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0005929 cilium | NAS PMID:32229580 Cytoplasmic dynein-2 at a glance. | MODIFY | Summary: The source describes cytoplasmic dynein-2 as the ciliary transport motor. Reason: The source describes cytoplasmic dynein-2 as the ciliary transport motor. WDR34 is a nonmembrane component of that assembly, so ciliary plasm is the informative refinement of cilium. The cached source is abstract-only; no unpublished spatial resolution or source-specific figure assay is inferred. Independent primary evidence supports the integrated location. Proposed replacements: ciliary plasm Supporting Evidence: file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0005929 cilium | TAS Reactome:R-HSA-5624949 | MODIFY | Summary: The saved Reactome anterograde-train assembly path reaches the literal human WDR34 entity 5624900 (Q96EX3, residues 1β536) through the dynein-2 complex. Reason: The saved Reactome anterograde-train assembly path reaches the literal human WDR34 entity 5624900 (Q96EX3, residues 1β536) through the dynein-2 complex. This supports the ciliary pool. Refine the broad cilium assignment to ciliary plasm using the nonmembrane motor-complex identity; the model compartment itself is cilium. Presence in an anterograde train means carriage of dynein-2 and does not imply that WDR34 powers anterograde motion. Proposed replacements: ciliary plasm Supporting Evidence: Reactome:R-HSA-5624949 file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0005929 cilium | TAS Reactome:R-HSA-5625416 | MODIFY | Summary: The saved Reactome anterograde IFT path reaches the literal human WDR34 entity 5624900 (Q96EX3, residues 1β536) through the dynein-2 complex. Reason: The saved Reactome anterograde IFT path reaches the literal human WDR34 entity 5624900 (Q96EX3, residues 1β536) through the dynein-2 complex. This supports the ciliary pool. Refine the broad cilium assignment to ciliary plasm using the nonmembrane motor-complex identity; the model compartment itself is cilium. Presence in an anterograde train means carriage of dynein-2 and does not imply that WDR34 powers anterograde motion. Proposed replacements: ciliary plasm Supporting Evidence: Reactome:R-HSA-5625416 file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0005929 cilium | TAS Reactome:R-HSA-5625424 | MODIFY | Summary: The saved Reactome retrograde-train dissociation path reaches the literal human WDR34 entity 5624900 (Q96EX3, residues 1β536) through the dynein-2 complex. Reason: The saved Reactome retrograde-train dissociation path reaches the literal human WDR34 entity 5624900 (Q96EX3, residues 1β536) through the dynein-2 complex. This supports the ciliary pool. Refine the broad cilium assignment to ciliary plasm using the nonmembrane motor-complex identity; the model compartment itself is cilium. Proposed replacements: ciliary plasm Supporting Evidence: Reactome:R-HSA-5625424 file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0005929 cilium | TAS Reactome:R-HSA-5625426 | MODIFY | Summary: The saved Reactome retrograde IFT path reaches the literal human WDR34 entity 5624900 (Q96EX3, residues 1β536) through the dynein-2 complex. Reason: The saved Reactome retrograde IFT path reaches the literal human WDR34 entity 5624900 (Q96EX3, residues 1β536) through the dynein-2 complex. This supports the ciliary pool. Refine the broad cilium assignment to ciliary plasm using the nonmembrane motor-complex identity; the model compartment itself is cilium. Proposed replacements: ciliary plasm Supporting Evidence: Reactome:R-HSA-5625426 file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0005930 axoneme | IEA GO_REF:0000120 | ACCEPT | Summary: The combined ortholog and UniProt axoneme mapping is supported by mouse Wdr34 localization and human ciliary WDR34 observations. Reason: The combined ortholog and UniProt axoneme mapping is supported by mouse Wdr34 localization and human ciliary WDR34 observations. Retain the axonemal association of the IFT motor. This does not assign WDR34 to an axonemal dynein arm, infer that it polymerizes microtubules, or imply permanently fixed attachment. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:Q5U4F6 SUPPORTS TRANSFER UniProtKB-SubCell:SL-0304 SUPPORTS TRANSFER Supporting Evidence: file:human/DYNC2I2/DYNC2I2-source-guide.md |
| GO:0005930 axoneme | ISS GO_REF:0000024 | ACCEPT | Summary: The verified mouse Wdr34 donor Q5U4F6 has direct axonemal/ciliary localization evidence. Reason: The verified mouse Wdr34 donor Q5U4F6 has direct axonemal/ciliary localization evidence. Human WDR34 also localizes along cilia and belongs to the IFT motor that travels on axonemal microtubules. Retain the ortholog-supported axonemal association while keeping it distinct from membership in a beating dynein arm. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:Q5U4F6 SUPPORTS TRANSFER |
| GO:0030175 filopodium | IEA GO_REF:0000120 | MARK AS OVER ANNOTATED | Summary: The recovered tagged-mouse observation is positive, but its transfer as a defined human filopodium location exceeds the resolved compartment evidence. Reason: PMID:28379358 reports sequence-checked mouse Wdr34-GFP in wild-type mouse fibroblasts, with cortical and filopodia-like fluorescence and a GFP-only control. This is a genuine positive observation. The reported GFP/acetylated-tubulin/DAPI imaging does not establish that the protrusions are the actin-based compartment defined by GO:0030175, and it does not establish endogenous human localization. Mark the electronic human transfer as over-annotated while retaining the experimental context. This does not declare the fluorescence artifactual or require a known filopodial function for a localization annotation. Propagation Review Root cause: SOURCE WEAK OR INFERRED Failure modes: CONTEXT OR TISSUE MISMATCH SOURCE EVIDENCE WEAK Sources checked: UniProtKB:Q5U4F6 SOURCE WEAK OR INFERRED The positive tagged-mouse observation is insufficient to establish a defined endogenous human filopodium pool. UniProtKB-SubCell:SL-0286 SOURCE WEAK OR INFERRED The location mapping does not add independent compartment-identification or human-localization evidence. |
| GO:0030286 dynein complex | NAS PMID:30065109 Emerging mechanisms of dynein transport in the cytoplasm ver... | MODIFY | Summary: This review identifies WDR34 as a dynein-2 intermediate chain. Reason: This review identifies WDR34 as a dynein-2 intermediate chain. Refine generic dynein complex to cytoplasmic dynein complex (GO:0005868), the current child that explicitly includes intraflagellar dynein-2. The refinement does not assert a nonciliary cytoplasmic location or axonemal arm membership. Proposed replacements: cytoplasmic dynein complex |
| GO:0030286 dynein complex | NAS PMID:32229580 Cytoplasmic dynein-2 at a glance. | MODIFY | Summary: The source specifically concerns cytoplasmic dynein-2 rather than an unspecified dynein family complex. Reason: The source specifically concerns cytoplasmic dynein-2 rather than an unspecified dynein family complex. Refine to GO:0005868, whose current definition includes IFT motors. The abstract-only source is treated as a named statement corroborated by human primary biochemical/structural evidence, not as a newly reconstructed experimental assay. Proposed replacements: cytoplasmic dynein complex Supporting Evidence: file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0035721 intraciliary retrograde transport | IEA GO_REF:0000120 | ACCEPT | Summary: The current ARBA00092633 rule matches the target WDR34 FunFam pair in the permitted eukaryotic lineage; mouse Q5U4F6 is also a genuine ortholog source. Reason: The current ARBA00092633 rule matches the target WDR34 FunFam pair in the permitted eukaryotic lineage; mouse Q5U4F6 is also a genuine ortholog source. Human WDR34 knockout/rescue and dynein-2 assembly experiments independently support contribution to retrograde ciliary transport. This is participation as an accessory motor subunit, not autonomous ATP hydrolysis. Propagation Review Root cause: NO FAILURE CORE Sources checked: ARBA:ARBA00092633 SUPPORTS TRANSFER UniProtKB:Q5U4F6 SUPPORTS TRANSFER Supporting Evidence: file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0035721 intraciliary retrograde transport | IMP PMID:29742051 Interaction of WDR60 intermediate chain with TCTEX1D2 light ... | ACCEPT | Summary: The original preserves WDR34 as part of the light-chain/intermediate-chain assembly linked to the heavy-chain subcomplex. Reason: The original preserves WDR34 as part of the light-chain/intermediate-chain assembly linked to the heavy-chain subcomplex. Its explicitly described knockout experiments concern WDR60 and TCTEX1D2; a WDR34-specific IMP experiment was not independently reconstructed from the recovered main text, and that limitation is retained. The assigned WDR34 process is nevertheless firmly supported by its structural participation and the direct WDR34 knockout/rescue study PMID:30649997, so the curator assertion is retained rather than rejected from incomplete source reconstruction. Supporting Evidence: PMID:30649997 These observations together indicate that interactions of WDR34 with both DYNLL1/DYNLL2 and DYNLRB1/DYNLRB2 are essential for dynein-2βmediated retrograde trafficking of ciliary proteins. file:human/DYNC2I2/DYNC2I2-source-guide.md |
| GO:0035721 intraciliary retrograde transport | IMP PMID:30649997 Interactions of the dynein-2 intermediate chain WDR34 with t... | ACCEPT | Summary: Human RPE1 WDR34 knockout cells accumulate IFT88 and GPR161 in cilia, and WT WDR34 rescues the defects whereas light-chain-site deletion constructs do not. Reason: Human RPE1 WDR34 knockout cells accumulate IFT88 and GPR161 in cilia, and WT WDR34 rescues the defects whereas light-chain-site deletion constructs do not. This supports a functional contribution to retrograde ciliary trafficking. The severity depends on clone/truncation position: early predicted nulls can retain elongated cilia, while later truncations produce vestigial cilia. No precise IFT velocity or autonomous WDR34 ATPase activity was measured. Supporting Evidence: PMID:30649997 Our analyses using WDR34-KO cell lines, and those exogenously expressing WDR34 constructs lacking light chainβbinding sites, showed the importance of these interactions in retrograde ciliary protein trafficking. file:human/DYNC2I2/DYNC2I2-source-guide.md |
| GO:0035721 intraciliary retrograde transport | NAS PMID:31451806 Structure of the dynein-2 complex and its assembly with intr... | ACCEPT | Summary: Human reconstituted dynein-2 contains WDR34 in the intermediate/light-chain assembly that organizes the motor tails and supports IFT-train engagement. Reason: Human reconstituted dynein-2 contains WDR34 in the intermediate/light-chain assembly that organizes the motor tails and supports IFT-train engagement. The complex-level mechanism supports this named retrograde-transport assertion. The block-deletion assay does not isolate the effect of WDR34 alone and is not interpreted as its autonomous motor activity. Supporting Evidence: file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0035721 intraciliary retrograde transport | NAS PMID:32229580 Cytoplasmic dynein-2 at a glance. | ACCEPT | Summary: The source identifies cytoplasmic dynein-2 as the retrograde ciliary transport motor. Reason: The source identifies cytoplasmic dynein-2 as the retrograde ciliary transport motor. WDR34 is an established component of that motor, with independent direct human knockout/rescue support. Retain the process as structural/molecular participation, while keeping the source as a secondary named statement and its cached access as abstract-only. |
| GO:0036064 ciliary basal body | IDA GO_REF:0000052 | ACCEPT | Summary: The actual HPA target page gives supported reliability specifically to basal body. Reason: The actual HPA target page gives supported reliability specifically to basal body. Human cell-line observations include serum-starved hTERT-RPE1 and other ciliated cultures. This corroborates the experimentally observed basal-body pool. The supported basal-body score is not extrapolated to uncertain nuclear locations. Supporting Evidence: file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0036064 ciliary basal body | IDA PMID:25205765 Subunit composition of the human cytoplasmic dynein-2 comple... | ACCEPT | Summary: Human WDR34 localization at the ciliary base and pericentrosomal region, together with the biochemical dynein-2 assembly, supports the basal-body-associated pool. Reason: Human WDR34 localization at the ciliary base and pericentrosomal region, together with the biochemical dynein-2 assembly, supports the basal-body-associated pool. The study distinguishes endogenous antibody and tagged-protein observations; the annotation does not require that all WDR34 reside there. Supporting Evidence: PMID:25205765 demonstrate the accumulation of mGFPβWDR34 at the base of the cilium with some labeling evident along its length file:human/DYNC2I2/DYNC2I2-source-guide.md |
| GO:0036064 ciliary basal body | IEA GO_REF:0000120 | ACCEPT | Summary: ARBA00044605 matches the target WDR34 FunFam pair in Chordata; the combined assertion also traces to mouse Wdr34 Q5U4F6. Reason: ARBA00044605 matches the target WDR34 FunFam pair in Chordata; the combined assertion also traces to mouse Wdr34 Q5U4F6. Direct human localization and supported HPA basal-body observations independently corroborate this location. The rule is a genuine current mapping, not sole evidence for the biology. Propagation Review Root cause: NO FAILURE CORE Sources checked: ARBA:ARBA00044605 SUPPORTS TRANSFER UniProtKB:Q5U4F6 SUPPORTS TRANSFER Supporting Evidence: file:human/DYNC2I2/DYNC2I2-source-guide.md |
| GO:0036064 ciliary basal body | ISS GO_REF:0000024 | ACCEPT | Summary: The donor is authentic mouse Wdr34 Q5U4F6, with direct centriole/basal-body-associated localization evidence in PMID:24183451. Reason: The donor is authentic mouse Wdr34 Q5U4F6, with direct centriole/basal-body-associated localization evidence in PMID:24183451. The same paper includes endogenous human fibroblast basal-body staining, and later human observations corroborate this pool. Retain the ortholog-supported assertion without calling every mouse assay a human assay. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:Q5U4F6 SUPPORTS TRANSFER |
| GO:0042073 intraciliary transport | IBA GO_REF:0000033 | MODIFY | Summary: The current tree places human WDR34 PTN002488185 below PTN000278083, and the literal IBD supports intraciliary transport with human, mouse, trypanosome and zebrafish descendant evidence. Reason: The current tree places human WDR34 PTN002488185 below PTN000278083, and the literal IBD supports intraciliary transport with human, mouse, trypanosome and zebrafish descendant evidence. The target self-donor is legitimate grounding. Refine the broad bidirectional transport term to intraciliary retrograde transport, the work performed by the dynein-2 motor to which WDR34 contributes. Carriage of dynein-2 on anterograde trains is not evidence that WDR34 itself powers that direction. This is a granularity refinement, not a rejection of the PAINT node. The inherited PMID:30320547 excerpt describes the effects of disrupting the motor on the coupled transport system; it does not assign anterograde force generation to WDR34. The WDR34 light-chain binding and rescue experiments in PMID:30649997 and the human dynein-2 structure in PMID:31451806 provide the direct mechanistic basis for the refinement. Propagation Review Root cause: TERM SCOPING PROBLEM Failure modes: GRANULARITY MISMATCH Sources checked: PANTHER:PTN000278083 SUPPORTS TRANSFER Literal current IBD and target descendant path verified in the saved PTHR12442 tree; target alignment sequence exactly matches official Q96EX3. Donor current records and source-specific limitations are preserved separately. Proposed replacements: intraciliary retrograde transport Supporting Evidence: PMID:30320547 Both proteins are required to maintain a functional transition zone and for efficient bidirectional intraflagellar transport. file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0045503 dynein light chain binding | IBA GO_REF:0000033 | ACCEPT | Summary: The literal root IBD at PTN000277823 propagates light-chain binding through the target lineage. Reason: The literal root IBD at PTN000277823 propagates light-chain binding through the target lineage. Current mouse and fly donor records provide experimental grounding, and human WDR34 itself has direct light-chain association and site-deletion evidence. The human self-donor is not circular. Retain the conserved binding function with the source-specific cellular extract versus structural assay distinctions. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000277823 SUPPORTS TRANSFER Literal current IBD and target descendant path verified in the saved PTHR12442 tree; target alignment sequence exactly matches official Q96EX3. Donor current records and source-specific limitations are preserved separately. Supporting Evidence: PMID:30649997 In this study, we demonstrated that the WDR34 intermediate chain interacts with the two light chains, DYNLL1/DYNLL2 and DYNLRB1/DYNLRB2, via its distinct sites. file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0045503 dynein light chain binding | IPI PMID:25830415 Tctex1d2 associates with short-rib polydactyly syndrome prot... | ACCEPT | Summary: The literal partner is the dynein light chain TCTEX1D2/Q8WW35. Reason: The literal partner is the dynein light chain TCTEX1D2/Q8WW35. The original includes endogenous human co-association and a cell-free radiolabeled TCTEX1D2 experiment with FLAG-WDR34; the C-terminal TCTEX1D2 fragment retained WDR34 association. Retain this positive light-chain-binding assertion rather than substitute a different LC8/roadblock pair. Later architectural preference for WDR60βTCTEX1D2 complexes does not erase this assay-specific result or prove a purified direct affinity. Supporting Evidence: PMID:25830415 Consistently, Tctex1d2 IPd Wdr60, Wdr34, and other Dync1 and Dync2 components file:human/DYNC2I2/DYNC2I2-source-guide.md |
| GO:0045504 dynein heavy chain binding | IBA GO_REF:0000033 | ACCEPT | Summary: The literal IBD at the family root PTN000277823 is experimentally grounded by Dictyostelium dicA/P54703 records and inherited by the human target branch. Reason: The literal IBD at the family root PTN000277823 is experimentally grounded by Dictyostelium dicA/P54703 records and inherited by the human target branch. Human recombinant dynein-2 structural evidence independently places the WDR34 beta-propeller against a heavy-chain tail, and cellular subcomplex assays support association. Retain heavy-chain binding; neither the distant donor nor a small donor count is a propagation defect. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000277823 SUPPORTS TRANSFER Literal current IBD and target descendant path verified in the saved PTHR12442 tree; target alignment sequence exactly matches official Q96EX3. Donor current records and source-specific limitations are preserved separately. Supporting Evidence: PMID:31451806 DHC2TAIL-A and -B, are bound by the Ξ²-propeller of WDR60 and WDR34 respectively, mainly via bundle 3 file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0060271 cilium assembly | IEA GO_REF:0000120 | ACCEPT | Summary: ARBA00027837 has a target-matched WDR34 FunFam condition set in Euarchontoglires, and the mouse donor has direct ciliogenesis evidence. Reason: ARBA00027837 has a target-matched WDR34 FunFam condition set in Euarchontoglires, and the mouse donor has direct ciliogenesis evidence. Human WDR34 is a structural component of the dynein-2 machinery needed for normal ciliary assembly and trafficking. Retain the existing assembly assertion without claiming that every knockout abolishes axoneme extension: early and late truncation models have different phenotypes. No new developmental process is inferred. Propagation Review Root cause: NO FAILURE CORE Sources checked: ARBA:ARBA00027837 SUPPORTS TRANSFER UniProtKB:Q5U4F6 SUPPORTS TRANSFER Supporting Evidence: PMID:30320547 we show that WDR34 KO cells can assemble a dynein-2 motor complex that binds IFT proteins yet fails to extend an axoneme, indicating complex function is stalled file:human/DYNC2I2/DYNC2I2-source-guide.md |
| GO:0060271 cilium assembly | IGI PMID:25205765 Subunit composition of the human cytoplasmic dynein-2 comple... | ACCEPT | Summary: The human RPE1 study shows WDR34/WDR60 interdependence and nonadditive depletion effects on ciliogenesis. Reason: The human RPE1 study shows WDR34/WDR60 interdependence and nonadditive depletion effects on ciliogenesis. As an assembled motor subunit, WDR34 contributes to machinery that supports ciliary construction and maintenance rather than serving merely as its substrate. Retain the existing IGI assertion and literal WDR60 partner. The assay does not show that WDR34 nucleates or polymerizes microtubules itself. Supporting Evidence: PMID:25205765 Of note, double depletion of both WDR34 and WDR60 did not further exacerbate the cilia phenotypes seen file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0097014 ciliary plasm | IBA GO_REF:0000033 | ACCEPT | Summary: The target lies below the actual WDR34 PAINT node PTN000278083. Reason: The target lies below the actual WDR34 PAINT node PTN000278083. Its mouse and algal FAP133 donor records support the ciliary interior, and the human protein is a nonmembrane dynein-2 subunit. GO:0097014 denotes the ciliary contents excluding the enclosing plasma membrane, not an assertion of soluble location outside cilia. Retain this appropriately scoped location. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000278083 SUPPORTS TRANSFER Literal current IBD and target descendant path verified in the saved PTHR12442 tree; target alignment sequence exactly matches official Q96EX3. Donor current records and source-specific limitations are preserved separately. Supporting Evidence: file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0097542 ciliary tip | TAS Reactome:R-HSA-5624952 | ACCEPT | Summary: The exact saved Reactome path for retrograde-train assembly reaches WDR34 entity 5625407 (Q96EX3, residues 1β536) in the ciliary-tip compartment. Reason: The exact saved Reactome path for retrograde-train assembly reaches WDR34 entity 5625407 (Q96EX3, residues 1β536) in the ciliary-tip compartment. This explicitly modeled tip state belongs to the principal dynein-2 transport cycle, including turnaround and assembly or departure of the retrograde motor train. Accept the location with its Reactome provenance; it does not constitute a new direct imaging or kinetic measurement of human WDR34 at the tip. Supporting Evidence: Reactome:R-HSA-5624952 file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0097542 ciliary tip | TAS Reactome:R-HSA-5625416 | ACCEPT | Summary: The exact saved Reactome path for arrival of the anterograde train reaches WDR34 entity 5625407 (Q96EX3, residues 1β536) in the ciliary-tip compartment. Reason: The exact saved Reactome path for arrival of the anterograde train reaches WDR34 entity 5625407 (Q96EX3, residues 1β536) in the ciliary-tip compartment. This explicitly modeled tip state belongs to the principal dynein-2 transport cycle, including turnaround and assembly or departure of the retrograde motor train. Accept the location with its Reactome provenance; it does not constitute a new direct imaging or kinetic measurement of human WDR34 at the tip. Carriage to the tip by the anterograde train does not imply that WDR34 powers anterograde motion. Supporting Evidence: Reactome:R-HSA-5625416 file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0097542 ciliary tip | TAS Reactome:R-HSA-5625421 | ACCEPT | Summary: The exact saved Reactome path for anterograde-train dissociation reaches WDR34 entity 5625407 (Q96EX3, residues 1β536) in the ciliary-tip compartment. Reason: The exact saved Reactome path for anterograde-train dissociation reaches WDR34 entity 5625407 (Q96EX3, residues 1β536) in the ciliary-tip compartment. This explicitly modeled tip state belongs to the principal dynein-2 transport cycle, including turnaround and assembly or departure of the retrograde motor train. Accept the location with its Reactome provenance; it does not constitute a new direct imaging or kinetic measurement of human WDR34 at the tip. Carriage to the tip by the anterograde train does not imply that WDR34 powers anterograde motion. Supporting Evidence: Reactome:R-HSA-5625421 file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
| GO:0097542 ciliary tip | TAS Reactome:R-HSA-5625426 | ACCEPT | Summary: The exact saved Reactome path for departure of the retrograde train reaches WDR34 entity 5625407 (Q96EX3, residues 1β536) in the ciliary-tip compartment. Reason: The exact saved Reactome path for departure of the retrograde train reaches WDR34 entity 5625407 (Q96EX3, residues 1β536) in the ciliary-tip compartment. This explicitly modeled tip state belongs to the principal dynein-2 transport cycle, including turnaround and assembly or departure of the retrograde motor train. Accept the location with its Reactome provenance; it does not constitute a new direct imaging or kinetic measurement of human WDR34 at the tip. Supporting Evidence: Reactome:R-HSA-5625426 file:human/DYNC2I2/DYNC2I2-bioinformatics/evidence-audit/RESULTS.md |
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Download this section (compressed HTML)Q: Does endogenous WDR34 regulate TAK1 signaling independently of dynein-2 and cilia, and can distinct sequence-verified rescue reagents resolve the proposed sequestration mechanism?
Q: Is the tagged mouse Wdr34 filopodial pool also present for endogenous human DYNC2I2, and what function does it serve?
Q: Which target-specific experimental evidence establishes the curated human male-germ-cell nuclear pool, beyond the positive mouse Wdr34 light-chain interaction in the same source?
Experiment: Compare NF-kappaB reporter activity and TAK1 phosphorylation after IL-1beta or LPS in WDR34-knockout versus WDR60-knockout and DYNC2H1-knockout cells. Rescue with WDR34 mutants that cannot bind dynein-2 light chains or heavy chain.
Hypothesis: WDR34 suppresses TLR/IL-1R NF-kappaB signalling independently of its dynein-2 role.
Type: knockout signalling assay
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