Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0070449 elongin complex	IBA GO_REF:0000033	ACCEPT	Summary: Correct. ELOB is the ubiquitin-like Elongin B subunit of the Elongin BC regulatory module and Elongin ABC complex. Supporting Evidence: file:human/ELOB/ELOB-deep-research-falcon.md for ELOB itself, the best-supported “primary function” in mechanistic terms is non-enzymatic—it serves as a ubiquitin-like adaptor/regulatory subunit within Elongin BC
GO:0030891 VCB complex	IBA GO_REF:0000033	ACCEPT	Summary: Correct. ELOB/ELOC form the Elongin BC module in the VHL-containing CRL2/VCB ubiquitin ligase complex. Supporting Evidence: file:human/ELOB/ELOB-deep-research-falcon.md In the CRL2^VHL ligase, the substrate receptor pVHL binds Elongin BC and engages CUL2–RBX1 to ubiquitinate substrates
GO:0000151 ubiquitin ligase complex	IEA GO_REF:0000117	MARK AS OVER ANNOTATED	Summary: Correct but broad. ELOB is a non-catalytic adaptor/regulatory subunit in more specific Cul2/Cul5 ubiquitin ligase complexes. Reason: More specific CRL2/CRL5 and VCB complex annotations capture the supported complex membership.
GO:0005634 nucleus	IEA GO_REF:0000044	ACCEPT	Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts.
GO:0005737 cytoplasm	IEA GO_REF:0000117	MARK AS OVER ANNOTATED	Summary: Correct but less specific than cytosol/nucleoplasm annotations for ELOB-containing complexes. Reason: Use cytosol and nucleoplasm where available rather than generic cytoplasm.
GO:0006368 transcription elongation by RNA polymerase II	IEA GO_REF:0000002	ACCEPT	Summary: Accepted. ELOB is a regulatory Elongin subunit in the Elongin ABC transcription elongation factor that promotes RNA polymerase II elongation. Supporting Evidence: file:human/ELOB/ELOB-deep-research-falcon.md Elongin was originally identified as a positive regulator of RNA polymerase II transcription elongation.
GO:0030891 VCB complex	IEA GO_REF:0000002	ACCEPT	Summary: Correct. ELOB/ELOC form the Elongin BC module in the VHL-containing CRL2/VCB ubiquitin ligase complex. Supporting Evidence: file:human/ELOB/ELOB-deep-research-falcon.md In the CRL2^VHL ligase, the substrate receptor pVHL binds Elongin BC and engages CUL2–RBX1 to ubiquitinate substrates
GO:0070449 elongin complex	IEA GO_REF:0000120	ACCEPT	Summary: Correct. ELOB is the ubiquitin-like Elongin B subunit of the Elongin BC regulatory module and Elongin ABC complex. Supporting Evidence: file:human/ELOB/ELOB-deep-research-falcon.md for ELOB itself, the best-supported “primary function” in mechanistic terms is non-enzymatic—it serves as a ubiquitin-like adaptor/regulatory subunit within Elongin BC
GO:0005515 protein binding	IPI PMID:10205047 Structure of the VHL-ElonginC-ElonginB complex: implications...	REMOVE	Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations.
GO:0005515 protein binding	IPI PMID:12004076 Structure of an HIF-1alpha -pVHL complex: hydroxyproline rec...	REMOVE	Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations.
GO:0005515 protein binding	IPI PMID:12050673 Structural basis for the recognition of hydroxyproline in HI...	REMOVE	Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations.
GO:0005515 protein binding	IPI PMID:12149480 Mammalian mediator subunit mMED8 is an Elongin BC-interactin...	REMOVE	Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations.
GO:0005515 protein binding	IPI PMID:15601820 VHL-box and SOCS-box domains determine binding specificity f...	REMOVE	Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations.
GO:0005515 protein binding	IPI PMID:16189514 Towards a proteome-scale map of the human protein-protein in...	REMOVE	Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations.
GO:0005515 protein binding	IPI PMID:18323857 NEDD8 acts as a 'molecular switch' defining the functional s...	REMOVE	Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations.
GO:0005515 protein binding	IPI PMID:20211142 An atlas of combinatorial transcriptional regulation in mous...	REMOVE	Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations.
GO:0005515 protein binding	IPI PMID:20871634 Regulation of receptor for activated C kinase 1 protein by t...	REMOVE	Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations.
GO:0005515 protein binding	IPI PMID:21516116 Next-generation sequencing to generate interactome datasets.	REMOVE	Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations.
GO:0005515 protein binding	IPI PMID:22190034 Global landscape of HIV-human protein complexes.	REMOVE	Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations.
GO:0005515 protein binding	IPI PMID:25416956 A proteome-scale map of the human interactome network.	REMOVE	Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations.
GO:0005515 protein binding	IPI PMID:27517496 USP9X destabilizes pVHL and promotes cell proliferation.	REMOVE	Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations.
GO:0005515 protein binding	IPI PMID:30026228 VHL substrate transcription factor ZHX2 as an oncogenic driv...	REMOVE	Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations.
GO:0005515 protein binding	IPI PMID:32296183 A reference map of the human binary protein interactome.	REMOVE	Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations.
GO:0005515 protein binding	IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling...	REMOVE	Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations.
GO:0005515 protein binding	IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce...	REMOVE	Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations.
GO:0005515 protein binding	IPI PMID:40205054 Multimodal cell maps as a foundation for structural and func...	REMOVE	Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations.
GO:0016567 protein ubiquitination	IEA GO_REF:0000041	ACCEPT	Summary: Supported as a broad process enabled by ELOB-containing CRL2/CRL5 ligases, though ELOB itself is a non-catalytic Elongin BC adaptor/regulatory subunit, not a substrate receptor or ubiquitin-transfer enzyme. Reason: Keep the process annotation because ELOB is a direct required module in CRL ubiquitination complexes. Do not add GO:1990756 for ELOB itself, because substrate recognition is supplied by SOCS-box, VHL-box, or related substrate receptor proteins rather than by ELOB. Supporting Evidence: file:human/ELOB/ELOB-deep-research-falcon.md Elongin BC acts as an adaptor that couples substrate-recognition proteins bearing a VHL box or SOCS box to a Cullin scaffold
GO:0031466 Cul5-RING ubiquitin ligase complex	IEA GO_REF:0000107	ACCEPT	Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors.
GO:0031466 Cul5-RING ubiquitin ligase complex	IDA PMID:40963025 Covariation MS uncovers a protein that controls cysteine cat...	ACCEPT	Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors.
GO:0000122 negative regulation of transcription by RNA polymerase II	NAS PMID:28591624 Crystal Structure of the Cul2-Rbx1-EloBC-VHL Ubiquitin Ligas...	KEEP AS NON CORE	Summary: Context-specific transcriptional regulation annotation. ELOB core biology is Elongin/CRL adaptor function rather than a dedicated transcriptional repressor. Reason: Non-core transcription regulatory consequence of Elongin-containing complexes.
GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process	NAS PMID:31273098 A glycine-specific N-degron pathway mediates the quality con...	KEEP AS NON CORE	Summary: Supported as a broad consequence of ELOB-containing Cullin-RING ligase activity, but less specific than complex/adaptor annotations. Reason: ELOB is a non-catalytic adaptor/regulatory subunit within the ubiquitin ligase machinery.
GO:0140627 ubiquitin-dependent protein catabolic process via the C-end degron rule pathway	NAS PMID:15601820 VHL-box and SOCS-box domains determine binding specificity f...	KEEP AS NON CORE	Summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin ligase complexes, but not the general core molecular role of ELOB. Reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex function.
GO:0140627 ubiquitin-dependent protein catabolic process via the C-end degron rule pathway	NAS PMID:21822215 The tumour antigen PRAME is a subunit of a Cul2 ubiquitin li...	KEEP AS NON CORE	Summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin ligase complexes, but not the general core molecular role of ELOB. Reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex function.
GO:0140627 ubiquitin-dependent protein catabolic process via the C-end degron rule pathway	NAS PMID:22649776 The Role of Elongin BC-Containing Ubiquitin Ligases.	KEEP AS NON CORE	Summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin ligase complexes, but not the general core molecular role of ELOB. Reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex function.
GO:0140627 ubiquitin-dependent protein catabolic process via the C-end degron rule pathway	NAS PMID:29779948 The Eukaryotic Proteome Is Shaped by E3 Ubiquitin Ligases Ta...	KEEP AS NON CORE	Summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin ligase complexes, but not the general core molecular role of ELOB. Reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex function.
GO:0140627 ubiquitin-dependent protein catabolic process via the C-end degron rule pathway	NAS PMID:33398168 Molecular basis for arginine C-terminal degron recognition b...	KEEP AS NON CORE	Summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin ligase complexes, but not the general core molecular role of ELOB. Reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex function.
GO:0140627 ubiquitin-dependent protein catabolic process via the C-end degron rule pathway	NAS PMID:34743205 CRL2-KLHDC3 E3 ubiquitin ligase complex suppresses ferroptos...	KEEP AS NON CORE	Summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin ligase complexes, but not the general core molecular role of ELOB. Reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex function.
GO:1990116 ribosome-associated ubiquitin-dependent protein catabolic process	NAS PMID:33909987 Convergence of mammalian RQC and C-end rule proteolytic path...	KEEP AS NON CORE	Summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin ligase complexes, but not the general core molecular role of ELOB. Reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex function.
GO:2000104 negative regulation of DNA-templated DNA replication	NAS PMID:22649776 The Role of Elongin BC-Containing Ubiquitin Ligases.	KEEP AS NON CORE	Summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin ligase complexes, but not the general core molecular role of ELOB. Reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex function.
GO:0031466 Cul5-RING ubiquitin ligase complex	IDA PMID:39039081 Molecular insights into degron recognition by CRL5(ASB7) ubi...	ACCEPT	Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors.
GO:0031466 Cul5-RING ubiquitin ligase complex	IDA PMID:40440427 ASB7 is a negative regulator of H3K9me3 homeostasis.	ACCEPT	Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors.
GO:0031462 Cul2-RING ubiquitin ligase complex	IDA PMID:35978186 Post-translational control of beige fat biogenesis by PRDM16...	ACCEPT	Summary: Correct. ELOB participates in Cul2-RING ubiquitin ligase assemblies such as CRL2-VHL through the Elongin BC adaptor module. Supporting Evidence: file:human/ELOB/ELOB-deep-research-falcon.md In the CRL2^VHL ligase, the substrate receptor pVHL binds Elongin BC and engages CUL2–RBX1 to ubiquitinate substrates
GO:0031462 Cul2-RING ubiquitin ligase complex	IDA PMID:37844242 Molecular basis for C-degron recognition by CRL2(APPBP2) ubi...	ACCEPT	Summary: Correct. ELOB participates in Cul2-RING ubiquitin ligase assemblies such as CRL2-VHL through the Elongin BC adaptor module. Supporting Evidence: file:human/ELOB/ELOB-deep-research-falcon.md In the CRL2^VHL ligase, the substrate receptor pVHL binds Elongin BC and engages CUL2–RBX1 to ubiquitinate substrates
GO:0140627 ubiquitin-dependent protein catabolic process via the C-end degron rule pathway	IDA PMID:37844242 Molecular basis for C-degron recognition by CRL2(APPBP2) ubi...	KEEP AS NON CORE	Summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin ligase complexes, but not the general core molecular role of ELOB. Reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex function.
GO:0031466 Cul5-RING ubiquitin ligase complex	IDA PMID:35512830 Rab40c regulates focal adhesions and PP6 activity by control...	ACCEPT	Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors.
GO:0031466 Cul5-RING ubiquitin ligase complex	IDA PMID:37816714 Structure-based design of a phosphotyrosine-masked covalent ...	ACCEPT	Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors.
GO:0031466 Cul5-RING ubiquitin ligase complex	IDA PMID:34857742 Discovery of an exosite on the SOCS2-SH2 domain that enhance...	ACCEPT	Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors.
GO:0031466 Cul5-RING ubiquitin ligase complex	IDA PMID:21980433 The SOCS2 ubiquitin ligase complex regulates growth hormone ...	ACCEPT	Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors.
GO:0031466 Cul5-RING ubiquitin ligase complex	IDA PMID:25505247 Biophysical studies on interactions and assembly of full-siz...	ACCEPT	Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors.
GO:0031466 Cul5-RING ubiquitin ligase complex	IDA PMID:38418882 The CRL5-SPSB3 ubiquitin ligase targets nuclear cGAS for deg...	ACCEPT	Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors.
GO:0031466 Cul5-RING ubiquitin ligase complex	IDA PMID:31387940 BIK ubiquitination by the E3 ligase Cul5-ASB11 determines ce...	ACCEPT	Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors.
GO:0031466 Cul5-RING ubiquitin ligase complex	IDA PMID:32513959 Structure and dynamics of the ASB9 CUL-RING E3 Ligase.	ACCEPT	Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors.
GO:0031466 Cul5-RING ubiquitin ligase complex	IDA PMID:23837592 Multimeric complexes among ankyrin-repeat and SOCS-box prote...	ACCEPT	Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors.
GO:0031466 Cul5-RING ubiquitin ligase complex	IDA PMID:33268465 The Mechanism of NEDD8 Activation of CUL5 Ubiquitin E3 Ligas...	ACCEPT	Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors.
GO:0140958 target-directed miRNA degradation	IMP PMID:33184237 The ZSWIM8 ubiquitin ligase mediates target-directed microRN...	KEEP AS NON CORE	Summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin ligase complexes, but not the general core molecular role of ELOB. Reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex function.
GO:0005515 protein binding	IPI PMID:22510880 Nuclear receptor binding protein 1 regulates intestinal prog...	REMOVE	Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations.
GO:0001222 transcription corepressor binding	IPI PMID:7660122 Inhibition of transcription elongation by the VHL tumor supp...	KEEP AS NON CORE	Summary: Supported as a binding/regulatory-context annotation but secondary to ELOB's broader Elongin BC adaptor role. Reason: Non-core interaction-specific molecular function.
GO:0006367 transcription initiation at RNA polymerase II promoter	IDA PMID:7660122 Inhibition of transcription elongation by the VHL tumor supp...	REMOVE	Summary: Remove. The cited PMID:7660122 paper concerns VHL inhibition of Elongin-dependent RNA polymerase II transcription elongation, not transcription initiation. Reason: Evidence-term mismatch: PMID:7660122 supports transcription elongation biology, not GO:0006367 transcription initiation at RNA polymerase II promoter.
GO:0070449 elongin complex	IDA PMID:7660122 Inhibition of transcription elongation by the VHL tumor supp...	ACCEPT	Summary: Correct. ELOB is the ubiquitin-like Elongin B subunit of the Elongin BC regulatory module and Elongin ABC complex. Supporting Evidence: file:human/ELOB/ELOB-deep-research-falcon.md for ELOB itself, the best-supported “primary function” in mechanistic terms is non-enzymatic—it serves as a ubiquitin-like adaptor/regulatory subunit within Elongin BC
GO:0005515 protein binding	IPI PMID:21119685 Notch-induced Asb2 expression promotes protein ubiquitinatio...	REMOVE	Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations.
GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process	ISS GO_REF:0000024	KEEP AS NON CORE	Summary: Supported as a broad consequence of ELOB-containing Cullin-RING ligase activity, but less specific than complex/adaptor annotations. Reason: ELOB is a non-catalytic adaptor/regulatory subunit within the ubiquitin ligase machinery.
GO:0005515 protein binding	IPI PMID:23401859 DCNL1 functions as a substrate sensor and activator of culli...	REMOVE	Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations.
GO:0005515 protein binding	IPI PMID:26906416 Characterization of the mammalian family of DCN-type NEDD8 E...	REMOVE	Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations.
GO:0005829 cytosol	TAS Reactome:R-HSA-1234159	ACCEPT	Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies.
GO:0005829 cytosol	TAS Reactome:R-HSA-1234163	ACCEPT	Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies.
GO:0005829 cytosol	TAS Reactome:R-HSA-1234173	ACCEPT	Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies.
GO:0005829 cytosol	TAS Reactome:R-HSA-1234175	ACCEPT	Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies.
GO:0005829 cytosol	TAS Reactome:R-HSA-1234177	ACCEPT	Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies.
GO:0005829 cytosol	TAS Reactome:R-HSA-1234183	ACCEPT	Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies.
GO:0005829 cytosol	TAS Reactome:R-HSA-180540	ACCEPT	Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies.
GO:0005829 cytosol	TAS Reactome:R-HSA-180555	ACCEPT	Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies.
GO:0005829 cytosol	TAS Reactome:R-HSA-180603	ACCEPT	Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies.
GO:0005829 cytosol	TAS Reactome:R-HSA-8952039	ACCEPT	Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies.
GO:0005829 cytosol	TAS Reactome:R-HSA-8952044	ACCEPT	Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies.
GO:0005829 cytosol	TAS Reactome:R-HSA-8952625	ACCEPT	Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies.
GO:0005829 cytosol	TAS Reactome:R-HSA-8952626	ACCEPT	Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies.
GO:0005829 cytosol	TAS Reactome:R-HSA-8955241	ACCEPT	Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies.
GO:0005829 cytosol	TAS Reactome:R-HSA-8955289	ACCEPT	Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies.
GO:0005829 cytosol	TAS Reactome:R-HSA-8956040	ACCEPT	Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies.
GO:0005829 cytosol	TAS Reactome:R-HSA-8956099	ACCEPT	Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies.
GO:0005829 cytosol	TAS Reactome:R-HSA-8956103	ACCEPT	Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies.
GO:0005829 cytosol	TAS Reactome:R-HSA-8956106	ACCEPT	Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies.
GO:0005829 cytosol	TAS Reactome:R-HSA-9705738	ACCEPT	Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies.
GO:0005829 cytosol	TAS Reactome:R-HSA-9755303	ACCEPT	Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies.
GO:0005829 cytosol	TAS Reactome:R-HSA-9833107	ACCEPT	Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies.
GO:0005829 cytosol	TAS Reactome:R-HSA-9833155	ACCEPT	Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies.
GO:0005829 cytosol	TAS Reactome:R-HSA-9954721	ACCEPT	Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies.
GO:0005829 cytosol	TAS Reactome:R-HSA-9954723	ACCEPT	Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-112379	ACCEPT	Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-112385	ACCEPT	Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-112392	ACCEPT	Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-112395	ACCEPT	Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-112396	ACCEPT	Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-112435	ACCEPT	Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-112436	ACCEPT	Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-113411	ACCEPT	Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-113412	ACCEPT	Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-113413	ACCEPT	Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-113414	ACCEPT	Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-113429	ACCEPT	Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-1234169	ACCEPT	Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-1234172	ACCEPT	Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-1234175	ACCEPT	Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-6797606	ACCEPT	Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-6797616	ACCEPT	Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts.
GO:0031625 ubiquitin protein ligase binding	IDA PMID:17636018 ASB4 is a hydroxylation substrate of FIH and promotes vascul...	ACCEPT	Summary: Accepted. ELOB binds and stabilizes ubiquitin ligase assemblies as part of the Elongin BC adaptor module. Supporting Evidence: file:human/ELOB/ELOB-deep-research-falcon.md Elongin BC acts as an adaptor that couples substrate-recognition proteins bearing a VHL box or SOCS box to a Cullin scaffold
GO:0031462 Cul2-RING ubiquitin ligase complex	IDA PMID:17636018 ASB4 is a hydroxylation substrate of FIH and promotes vascul...	ACCEPT	Summary: Correct. ELOB participates in Cul2-RING ubiquitin ligase assemblies such as CRL2-VHL through the Elongin BC adaptor module. Supporting Evidence: file:human/ELOB/ELOB-deep-research-falcon.md In the CRL2^VHL ligase, the substrate receptor pVHL binds Elongin BC and engages CUL2–RBX1 to ubiquitinate substrates
GO:0031466 Cul5-RING ubiquitin ligase complex	IDA PMID:17636018 ASB4 is a hydroxylation substrate of FIH and promotes vascul...	ACCEPT	Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors.
GO:0005515 protein binding	IPI PMID:15590694 Suppressors of cytokine signaling 4 and 5 regulate epidermal...	REMOVE	Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations.
GO:0006368 transcription elongation by RNA polymerase II	TAS PMID:7660129 Elongin (SIII): a multisubunit regulator of elongation by RN...	ACCEPT	Summary: Accepted. ELOB is a regulatory Elongin subunit in the Elongin ABC transcription elongation factor that promotes RNA polymerase II elongation. Supporting Evidence: file:human/ELOB/ELOB-deep-research-falcon.md Elongin was originally identified as a positive regulator of RNA polymerase II transcription elongation.
GO:0065003 protein-containing complex assembly	TAS PMID:7660129 Elongin (SIII): a multisubunit regulator of elongation by RN...	ACCEPT	Summary: Accepted. ELOB helps assemble/stabilize Elongin BC-containing CRL complexes and transcription elongation complexes. Supporting Evidence: file:human/ELOB/ELOB-deep-research-falcon.md for ELOB itself, the best-supported “primary function” in mechanistic terms is non-enzymatic—it serves as a ubiquitin-like adaptor/regulatory subunit within Elongin BC file:human/ELOB/ELOB-deep-research-falcon.md Elongin BC acts as an adaptor that couples substrate-recognition proteins bearing a VHL box or SOCS box to a Cullin scaffold

Core Functions

ELOB is a non-catalytic ubiquitin-ligase adaptor/regulatory subunit of Elongin BC that stabilizes transcription elongation and Cullin-RING ubiquitin ligase assemblies rather than acting as an enzyme or substrate receptor itself.

Molecular Function:

ubiquitin protein ligase binding

Directly Involved In:

transcription elongation by RNA polymerase II protein ubiquitination protein-containing complex assembly

Cellular Locations:

nucleoplasm cytosol

In Complex:

elongin complex

Supporting Evidence:

file:human/ELOB/ELOB-deep-research-falcon.md
for ELOB itself, the best-supported “primary function” in mechanistic terms is **non-enzymatic**—it serves as a **ubiquitin-like adaptor/regulatory subunit** within Elongin BC
file:human/ELOB/ELOB-deep-research-falcon.md
Elongin BC acts as an **adaptor** that couples substrate-recognition proteins bearing a **VHL box** or **SOCS box** to a Cullin scaffold
file:human/ELOB/ELOB-deep-research-falcon.md
**Elongin** was originally identified as a positive regulator of **RNA polymerase II** transcription elongation.

References

GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

GO_REF:0000024

Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000041

Gene Ontology annotation based on UniPathway vocabulary mapping

GO_REF:0000044

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000107

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:10205047

Structure of the VHL-ElonginC-ElonginB complex: implications for VHL tumor suppressor function.

PMID:12004076

Structure of an HIF-1alpha -pVHL complex: hydroxyproline recognition in signaling.

PMID:12050673

Structural basis for the recognition of hydroxyproline in HIF-1 alpha by pVHL.

PMID:12149480

Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase.

PMID:15590694

Suppressors of cytokine signaling 4 and 5 regulate epidermal growth factor receptor signaling.

PMID:15601820

VHL-box and SOCS-box domains determine binding specificity for Cul2-Rbx1 and Cul5-Rbx2 modules of ubiquitin ligases.

PMID:16189514

Towards a proteome-scale map of the human protein-protein interaction network.

PMID:17636018

ASB4 is a hydroxylation substrate of FIH and promotes vascular differentiation via an oxygen-dependent mechanism.

PMID:18323857

NEDD8 acts as a 'molecular switch' defining the functional selectivity of VHL.

PMID:20211142

An atlas of combinatorial transcriptional regulation in mouse and man.

PMID:20871634

Regulation of receptor for activated C kinase 1 protein by the von Hippel-Lindau tumor suppressor in IGF-I-induced renal carcinoma cell invasiveness.

PMID:21119685

Notch-induced Asb2 expression promotes protein ubiquitination by forming non-canonical E3 ligase complexes.

PMID:21516116

Next-generation sequencing to generate interactome datasets.

PMID:21822215

The tumour antigen PRAME is a subunit of a Cul2 ubiquitin ligase and associates with active NFY promoters.

PMID:21980433

The SOCS2 ubiquitin ligase complex regulates growth hormone receptor levels.

PMID:22190034

Global landscape of HIV-human protein complexes.

PMID:22510880

Nuclear receptor binding protein 1 regulates intestinal progenitor cell homeostasis and tumour formation.

PMID:22649776

The Role of Elongin BC-Containing Ubiquitin Ligases.

PMID:23401859

DCNL1 functions as a substrate sensor and activator of cullin 2-RING ligase.

PMID:23837592

Multimeric complexes among ankyrin-repeat and SOCS-box protein 9 (ASB9), ElonginBC, and Cullin 5: insights into the structure and assembly of ECS-type Cullin-RING E3 ubiquitin ligases.

PMID:25416956

A proteome-scale map of the human interactome network.

PMID:25505247

Biophysical studies on interactions and assembly of full-size E3 ubiquitin ligase: suppressor of cytokine signaling 2 (SOCS2)-elongin BC-cullin 5-ring box protein 2 (RBX2).

PMID:26906416

Characterization of the mammalian family of DCN-type NEDD8 E3 ligases.

PMID:27517496

USP9X destabilizes pVHL and promotes cell proliferation.

PMID:28591624

Crystal Structure of the Cul2-Rbx1-EloBC-VHL Ubiquitin Ligase Complex.

PMID:29779948

The Eukaryotic Proteome Is Shaped by E3 Ubiquitin Ligases Targeting C-Terminal Degrons.

PMID:30026228

VHL substrate transcription factor ZHX2 as an oncogenic driver in clear cell renal cell carcinoma.

PMID:31273098

A glycine-specific N-degron pathway mediates the quality control of protein N-myristoylation.

PMID:31387940

BIK ubiquitination by the E3 ligase Cul5-ASB11 determines cell fate during cellular stress.

PMID:32296183

A reference map of the human binary protein interactome.

PMID:32513959

Structure and dynamics of the ASB9 CUL-RING E3 Ligase.

PMID:33184237

The ZSWIM8 ubiquitin ligase mediates target-directed microRNA degradation.

PMID:33268465

The Mechanism of NEDD8 Activation of CUL5 Ubiquitin E3 Ligases.

PMID:33398168

Molecular basis for arginine C-terminal degron recognition by Cul2(FEM1) E3 ligase.

PMID:33909987

Convergence of mammalian RQC and C-end rule proteolytic pathways via alanine tailing.

PMID:33961781

Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.

PMID:34743205

CRL2-KLHDC3 E3 ubiquitin ligase complex suppresses ferroptosis through promoting p14(ARF) degradation.

PMID:34857742

Discovery of an exosite on the SOCS2-SH2 domain that enhances SH2 binding to phosphorylated ligands.

PMID:35271311

OpenCell: Endogenous tagging for the cartography of human cellular organization.

PMID:35512830

Rab40c regulates focal adhesions and PP6 activity by controlling ANKRD28 ubiquitylation.

PMID:35978186

Post-translational control of beige fat biogenesis by PRDM16 stabilization.

PMID:37816714

Structure-based design of a phosphotyrosine-masked covalent ligand targeting the E3 ligase SOCS2.

PMID:37844242

Molecular basis for C-degron recognition by CRL2(APPBP2) ubiquitin ligase.

PMID:38418882

The CRL5-SPSB3 ubiquitin ligase targets nuclear cGAS for degradation.

PMID:39039081

Molecular insights into degron recognition by CRL5(ASB7) ubiquitin ligase.

PMID:40205054

Multimodal cell maps as a foundation for structural and functional genomics.

PMID:40440427

ASB7 is a negative regulator of H3K9me3 homeostasis.

PMID:40963025

Covariation MS uncovers a protein that controls cysteine catabolism.

PMID:7660122

Inhibition of transcription elongation by the VHL tumor suppressor protein.

PMID:7660129

Elongin (SIII): a multisubunit regulator of elongation by RNA polymerase II.

Reactome:R-HSA-112379

Recruitment of elongation factors to form elongation complex

Reactome:R-HSA-112385

Addition of nucleotides leads to transcript elongation

Reactome:R-HSA-112392

Resumption of elongation after recovery from pausing

Reactome:R-HSA-112395

Abortive termination of elongation after arrest

Reactome:R-HSA-112396

Separation of elongating transcript from template

Reactome:R-HSA-112435

Formation of Elongin BC complex

Reactome:R-HSA-112436

Formation of Elongin complex

Reactome:R-HSA-113411

2-4 nt.backtracking of Pol II complex on the template leading to elongation pausing

Reactome:R-HSA-113412

Pol II elongation complex moves on the template as transcript elongates

Reactome:R-HSA-113413

TFIIS-mediated recovery of elongation from arrest

Reactome:R-HSA-113414

7-14 nt. Backtracking of Pol II complex on the template leading to elongation arrest

Reactome:R-HSA-113429

Elongating transcript encounters a lesion in the template

Reactome:R-HSA-1234159

Proteasome proteolyzes ub-HIF-alpha

Reactome:R-HSA-1234163

Cytosolic VBC complex ubiquitinylates hydroxyprolyl-HIF-alpha

Reactome:R-HSA-1234169

Nuclear VHL:EloB,C:CUL2:RBX1 binds hydroxyprolyl-HIF-alpha

Reactome:R-HSA-1234172

Nuclear VBC complex ubiquitinylates HIF-alpha

Reactome:R-HSA-1234173

Cytosolic PHD2,3 hydroxylates proline residues on HIF3A

Reactome:R-HSA-1234175

ub-hydroxyPro-HIF-alpha:VHL:EloB,C:CUL2:RBX1 translocates from the nucleus to the cytosol

Reactome:R-HSA-1234177

Cytosolic PHD2,3 hydroxylates proline residues on HIF1A

Reactome:R-HSA-1234183

Cytosolic VHL:EloB,C:CUL2:RBX1 binds hydroxyprolyl-HIF-alpha

Reactome:R-HSA-180540

Multi-ubiquitination of APOBEC3G

Reactome:R-HSA-180555

Association of APOBEC3G:Vif with the Cul5-SCF complex

Reactome:R-HSA-180603

Proteosome-mediated degradation of APOBEC3G

Reactome:R-HSA-6797606

CDK12 phosphorylates RNA Pol II CTD at DNA repair genes

Reactome:R-HSA-6797616

CCNK:CDK12 binds RNA Pol II at DNA repair genes

Reactome:R-HSA-8952039

NEDD8:AcM-UBE2F binds CRL5 E3 ubiquitin ligase complex

Reactome:R-HSA-8952044

AcM-UBE2F transfers NEDD8 to CRL5 E3 ubiquitin ligase complex

Reactome:R-HSA-8952625

NEDD8:AcM-UBE2M binds CRL2 E3 ubiquitin ligase complex

Reactome:R-HSA-8952626

AcM-UBE2M transfers NEDD8 to CRL2 E3 ubiquitin ligase complex

Reactome:R-HSA-8955241

CAND1 binds cytosolic CRL E3 ubiquitin ligases

Reactome:R-HSA-8955289

COMMDs displace CAND1 from cytosolic CRL E3 ubiquitin ligase complexes

Reactome:R-HSA-8956040

COP9 signalosome deneddylates cytosolic CRL E3 ubiquitin ligase complexes

Reactome:R-HSA-8956099

VHL:EloB,C:NEDD8-CUL2:RBX1 complex binds UBXN7

Reactome:R-HSA-8956103

VHL:EloB,C:NEDD8-CUL2:RBX1 complex binds hydroxyprolyl-HIF-alpha

Reactome:R-HSA-8956106

VHL:EloB,C:NEDD8-CUL2:RBX1 complex ubiquitinylates HIF-alpha

Reactome:R-HSA-9705738

SOCS1,3 ubiquitinates CSF3R in SOCS1,3:p-4Y-CSF3R:CSF3 dimer:LYN:p-Y-JAK1:p-JAK2:p-SYK:p-HCK:p-TYK2:CUL5:ELOB:ELOC:RNF7

Reactome:R-HSA-9755303

26S proteasome degrades HIFalpha

Reactome:R-HSA-9833107

Association of NS1 (1C) with Cul5 and SCF

Reactome:R-HSA-9833155

Ubiquitination of STAT2

Reactome:R-HSA-9954721

NEDD8-K699-CUL2:ELOB:ELOC:RBX1:KLHDC10 K48 polyubiquitinates alanine-tailed nascent peptide

Reactome:R-HSA-9954723

NEDD8-K699-CUL2:ELOB:ELOC:RBX1:KLHDC10 binds alanine-tailed nascent peptide

file:human/ELOB/ELOB-deep-research-falcon.md

Falcon deep research report for human ELOB

Suggested Experiments

Experiment: Rescue ELOB-depleted cells with interface mutants and compare RNA polymerase II elongation readouts, VHL-HIF turnover, and CRL5-SPSB3-dependent nuclear cGAS degradation.

Hypothesis: ELOB ubiquitin-like-domain surfaces differentially stabilize Elongin ABC versus CRL2/CRL5 assemblies.

Experiment: Reconstitute ELOBC-containing CRL2/CRL5 complexes with ELOB mutants and measure complex assembly, substrate binding, and ubiquitination kinetics.

Hypothesis: ELOB is required as a structural adaptor for substrate-receptor coupling rather than ubiquitin transfer catalysis.

Deep Research

Falcon

(ELOB-deep-research-falcon.md)

Research Report: Human **ELOB** (TCEB2; Elongin-B; UniProt **Q15370**) Falcon Edison Scientific Literature 22 citations 2026-05-11T14:05:45.093361

The research report should be a detailed narrative explaining the function, biological processes, and localization of the gene product. Citations should be given for all claims.

You should prioritize authoritative reviews and primary scientific literature when conducting research. You can supplement
this with annotations you find in gene/protein databases, but these can be outdated or inaccurate.

We are specifically interested in the primary function of the gene - for enzymes, what reaction is catalyzed, and what is the substrate specificity? For transporters, what is the substrate? For structural proteins or adapters, what is the broader structural role? For signaling molecules, what is the role in the pathway.

We are interested in where in or outside the cell the gene product carries out its function.

We are also interested in the signaling or biochemical pathways in which the gene functions. We are less interested in broad pleiotropic effects, except where these elucidate the precise role.

Include evidence where possible. We are interested in both experimental evidence as well as inference from structure, evolution, or bioinformatic analysis. Precise studies should be prioritized over high-throughput, where available.

Research Report: Human ELOB (TCEB2; Elongin-B; UniProt Q15370)

0. Target verification (critical identity check)

The UniProt accession Q15370 corresponds to human Elongin-B, encoded by ELOB (synonym TCEB2), also described historically as the RNA polymerase II transcription factor SIII subunit B (“SIII p18”) and “Elongin 18 kDa subunit”. The literature sources summarized below explicitly refer to Elongin B as TCEB2/ELOB and place it in the canonical Elongin BC heterodimer and in Cullin-RING ubiquitin ligase assemblies, consistent with the UniProt family/domain context (ubiquitin-like domain) and the expected human biology. (okumura2012theroleof pages 1-2, xu2024thecrl5–spsb3ubiquitin pages 2-3)

1. Key concepts and definitions (current understanding)

1.1 Elongin-B as a regulatory subunit of the Elongin transcription elongation factor

Elongin was originally identified as a positive regulator of RNA polymerase II transcription elongation. In this model, the complex includes a transcriptionally active subunit (Elongin A) and regulatory subunits Elongin B and Elongin C, which form the Elongin BC heterodimer. Elongin BC enhances Elongin A’s transcriptional activity and increases the elongation rate by suppressing transient pausing of RNA polymerase II. (okumura2012theroleof pages 1-2)

Functional definition: for ELOB itself, the best-supported “primary function” in mechanistic terms is non-enzymatic—it serves as a ubiquitin-like adaptor/regulatory subunit within Elongin BC, stabilizing/organizing complexes that modulate transcription elongation and, separately, assemble ubiquitin ligase machinery. (okumura2012theroleof pages 1-2, cai2016thestructureand pages 1-2)

1.2 Elongin BC as an adaptor module in Cullin-RING (CRL) ubiquitin ligases

A major modern view of ELOB biology is its role in ECS-family ligases (Elongin BC–Cullin–SOCS box/VHL box). Elongin BC acts as an adaptor that couples substrate-recognition proteins bearing a VHL box or SOCS box to a Cullin scaffold (typically CUL2 or CUL5) and a RING protein (RBX1/RBX2), yielding a functional Cullin-RING E3 ubiquitin ligase. (okumura2012theroleof pages 1-2, cai2016thestructureand pages 1-2)

Motif logic: VHL-box proteins contain a BC box that binds Elongin BC and a Cullin-specific module (e.g., a Cul2 box) that determines assembly with particular Cullins; this motif architecture explains how a conserved ELOB/ELOC adaptor can plug into multiple CRLs. (okumura2012theroleof pages 2-3, cai2016thestructureand pages 1-2)

1.3 Canonical pathway example: CRL2^VHL oxygen-sensing and HIF turnover

In the CRL2^VHL ligase, the substrate receptor pVHL binds Elongin BC and engages CUL2–RBX1 to ubiquitinate substrates—classically HIF-α—in an oxygen-dependent manner: prolyl hydroxylation of HIF-α enables pVHL recognition, leading to polyubiquitination and proteasomal degradation. This links ELOB (via Elongin BC) to the central oxygen-sensing/HIF pathway. (okumura2012theroleof pages 2-3, yan2004identificationofelongin pages 2-3)

2. Molecular functions, complexes, localization, and pathways

2.1 Major complexes containing ELOB

1) Elongin ABC (transcription elongation factor / SIII): ELOB + ELOC (Elongin BC) regulate Elongin A-dependent RNA polymerase II elongation by suppressing transient pausing. (Publication date: 2012-01; URL: https://doi.org/10.3389/fonc.2012.00010) (okumura2012theroleof pages 1-2)

2) CRL2^VHL (VCB-CR complex): pVHL + ELOB/ELOC + CUL2 + RBX1; key for ubiquitin-mediated control of HIF-α in oxygen sensing. (Publication date: 2004-10; URL: https://doi.org/10.1074/jbc.m408018200) (yan2004identificationofelongin pages 2-3)

3) CRL5/SOCS-box ligases: SOCS-box substrate receptors bind Elongin BC, which bridges them to CUL5–RBX2, enabling ubiquitination of diverse substrates in signaling and host-defense contexts. (Publication date: 2012-01; URL: https://doi.org/10.3389/fonc.2012.00010) (okumura2012theroleof pages 7-8, okumura2012theroleof pages 8-9)

4) CRL5–SPSB3 (innate immunity; nuclear cGAS turnover): a 2024 Nature study defines CRL5–SPSB3 where SPSB3 (substrate receptor) binds ELOBC, connecting to CUL5–RBX2 and promoting ubiquitylation/degradation of nuclear cGAS. (Publication date: 2024-02; URL: https://doi.org/10.1038/s41586-024-07112-w) (xu2024thecrl5–spsb3ubiquitin pages 2-3, xu2024thecrl5–spsb3ubiquitin pages 3-4)

2.2 Mechanistic notes and structural/biophysical constraints

Structural/biochemical analyses of CUL2-based ligases emphasize that Elongin BC is not a passive “tag-along” but a structural coupling module: in CRL2 assemblies, CUL2 binding involves defined hydrophobic/electrostatic contacts with the adaptor interface and can order loop residues in Elongin C, consistent with a tightly engineered assembly interface. (Publication date: 2016-03; URL: https://doi.org/10.1186/s13008-016-0020-7) (cai2016thestructureand pages 1-2)

3. Recent developments and latest research (prioritize 2023–2024)

3.1 2024: CRL5–SPSB3–ELOBC targets nuclear cGAS (high-confidence primary research)

Xu et al. (Nature, Feb 2024) performed an RNAi screen of 972 ubiquitin–proteasome system genes and found core CRL5 components—explicitly including TCEB2/ELOB—among top hits controlling nuclear cGAS abundance. (https://doi.org/10.1038/s41586-024-07112-w) (xu2024thecrl5–spsb3ubiquitin pages 2-3)

Mechanistically, the study shows that the SPSB3 substrate receptor binds Elongin BC through its SOCS box, linking SPSB3 to the CUL5–RBX2 catalytic core (thus requiring ELOB/ELOC as adaptor). (xu2024thecrl5–spsb3ubiquitin pages 2-3)

Quantitative/structural evidence:
- Bio-layer interferometry measured submicromolar affinity between cGAS and SPSB3–ELOBC (Kd = 352 nM for WT cGAS binding). (xu2024thecrl5–spsb3ubiquitin pages 5-6)
- A conserved NN degron in cGAS (Asn513/Asn514) is required for binding and in vitro ubiquitylation, demonstrating degron-encoded specificity at the adaptor-receptor interface. (xu2024thecrl5–spsb3ubiquitin pages 5-6)
- Mass spectrometry mapped ubiquitination sites and identified human cGAS Lys427/Lys428 as a dominant ubiquitinated pair; mutation of this pair largely abrogates ubiquitylation and nuclear degradation. (xu2024thecrl5–spsb3ubiquitin pages 3-4)

Visual support: the paper’s figures include a schematic of the CRL5–SPSB3 complex with ELOBC, RNAi screen hits listing TCEB2, and the BLI curve reporting Kd = 352 nM. (xu2024thecrl5–spsb3ubiquitin media d33342da, xu2024thecrl5–spsb3ubiquitin media abdf9a09, xu2024thecrl5–spsb3ubiquitin media 84ea615a)

3.2 2023: essentiality/fitness statistics for ELOB as a CRL2 subunit

Hanzl et al. (Nature Chemical Biology, Nov 2023) include ELOB among captured “CRL2 subunit” genes and report a median DepMap CRONOS score of −1.46 for ELOB (ELOC: −1.17; VHL: −0.96), providing a quantitative proxy for cellular fitness/essentiality trends across cancer cell lines. (https://doi.org/10.1038/s41589-022-01177-2) (hanzl2023functionale3ligase pages 1-4)

3.3 2024: reviews emphasizing CRL modularity and ELOB/ELOC adaptors in targeted protein degradation

A 2024 review on degrader discovery highlights CRL2^VHL as a commonly exploited ligase assembly (CUL2–RBX1–ElonginB–ElonginC–VHL) and emphasizes how adaptor modules (Elongin B/C) enable interchangeability of substrate receptors—an organizing principle in PROTAC design. (Publication date: 2024-03; URL: https://doi.org/10.3390/cells13070578) (bouvier2024breakingbadproteins—discovery pages 7-9)

A 2024 structural biology perspective on Cullin ligases discusses cryo-EM structures where the CUL2 N-terminus binds Elongins B and C, and it summarizes quantitative kinetic principles of CRL activation (e.g., neddylation accelerating CRL reactions and priming vs elongation rate differences) in the context of CRL2 systems used for PROTACs. (Publication date: 2024-10; URL: https://doi.org/10.1016/j.sbi.2024.102879) (lin2024diversityofstructure pages 3-5)

4. Current applications and real-world implementations

4.1 Targeted protein degradation (TPD): VHL/CRL2 systems as a widely used “E3 module”

Although ELOB is rarely the direct pharmacologic target, ELOB-containing assemblies are central enabling components in targeted protein degradation because many PROTACs recruit VHL, which operates in the CRL2^VHL complex containing Elongin B and C adaptor subunits. Reviews describe the canonical ligase composition (CUL2–RBX1–ElonginB–ElonginC–VHL) as the functional unit exploited in PROTACs. (bouvier2024breakingbadproteins—discovery pages 7-9)

4.2 Infection and viral immune evasion

Elongin BC (and therefore ELOB) is also used by viral proteins carrying SOCS-/BC-box motifs to assemble CRL-like ligases. For example, HIV-1 Vif recruits a CUL5-type ligase that polyubiquitinates APOBEC restriction factors for degradation, illustrating a real biological implementation of ELOB-containing CRL5 machinery in pathogen-host conflict. (okumura2012theroleof pages 7-8)

4.3 Oxygen-sensing and cancer biology via VHL/HIF axis

Mutations in VHL that disrupt the BC-box interaction (i.e., impairing Elongin BC binding) are strongly represented in VHL disease and sporadic clear-cell RCC contexts: one review reports that >70% of VHL disease and sporadic ccRCC VHL mutations involve BC-box defects affecting Elongin BC binding, and that ~57% of sporadic ccRCC have inactivating VHL mutations (with ~98% of those involving LOH). These statistics highlight the clinical importance of the ELOB/ELOC adaptor interface indirectly through VHL. (Publication date: 2012-01; URL: https://doi.org/10.3389/fonc.2012.00010) (okumura2012theroleof pages 2-3)

5. Expert opinions and analysis (authoritative synthesis)

5.1 Two-function model: transcription elongation factor vs ubiquitin-ligase adaptor

Authoritative reviews consolidate ELOB’s biology into two interconnected themes:
1) Transcription elongation regulation (as part of Elongin ABC), and
2) Adaptor-mediated ubiquitination (as part of Elongin BC within ECS/CRL2/CRL5 ligases).

A key interpretive point is that ELOB’s ubiquitin-like fold and stable heterodimerization with ELOC makes it suitable for repeated reuse as a plug-in adaptor: rather than conferring substrate specificity itself, ELOB supports assembly, stability, and coupling of specificity modules (VHL-box/SOCS-box proteins) to catalytic CRL cores (CUL2/CUL5 + RBX). (okumura2012theroleof pages 1-2, cai2016thestructureand pages 1-2)

5.2 “Modern” relevance: adaptor modules in immunity and precision protein control

The 2024 Nature work illustrates that ELOB’s adaptor role is not limited to classical hypoxia pathways. It can also be essential in nuclear innate immunity homeostasis, where a specific CRL5 substrate receptor (SPSB3) uses ELOBC to enforce degradation of nuclear cGAS. The integration of (i) screening, (ii) cryo-EM modelling, and (iii) quantitative biophysics provides a strong case that ELOBC-containing assemblies are “programmable” modules controlling the stability of nuclear sensors. (xu2024thecrl5–spsb3ubiquitin pages 2-3, xu2024thecrl5–spsb3ubiquitin pages 4-5, xu2024thecrl5–spsb3ubiquitin pages 5-6)

6. Relevant statistics and data highlights

cGAS–SPSB3–ELOBC binding affinity: Kd = 352 nM (BLI). (Xu et al., Nature, 2024-02; https://doi.org/10.1038/s41586-024-07112-w) (xu2024thecrl5–spsb3ubiquitin pages 5-6, xu2024thecrl5–spsb3ubiquitin media 84ea615a)
Ubiquitination-site mapping: dominant cGAS Lys427/Lys428 ubiquitination pair in human, supported by MS and mutational loss-of-function. (Xu et al., Nature, 2024-02; https://doi.org/10.1038/s41586-024-07112-w) (xu2024thecrl5–spsb3ubiquitin pages 3-4)
RNAi screen design/statistics: focused RNAi screen of 972 UPS-associated genes; CRL5 core components including TCEB2/ELOB were top hits (hits defined as >3 s.d. increase in cGAS–GFP). (Xu et al., Nature, 2024-02; https://doi.org/10.1038/s41586-024-07112-w) (xu2024thecrl5–spsb3ubiquitin pages 2-3)
Cancer-cell-line fitness proxy: median DepMap CRONOS score −1.46 reported for ELOB (CRL2 subunit) in a 2023 degrader-resistance/hotspot analysis dataset. (Hanzl et al., Nat Chem Biol, 2023-11; https://doi.org/10.1038/s41589-022-01177-2) (hanzl2023functionale3ligase pages 1-4)
VHL mutation statistics (clinical relevance of Elongin BC binding interface): review-reported >70% of VHL disease and sporadic ccRCC associated VHL mutations disrupt the BC-box interaction; ~57% of sporadic ccRCC show inactivating VHL mutations with ~98% LOH among those. (Okumura et al., Front Oncol, 2012-01; https://doi.org/10.3389/fonc.2012.00010) (okumura2012theroleof pages 2-3)

7. Consolidated evidence map (artifact)

The following table consolidates ELOB functions, complexes, pathways, and quantitative evidence with DOIs/URLs.

ELOB role/category	Major complex	Key partners	Biological process / pathway	Key evidence points (including quantitative details)	Year	DOI URL	Citation
Regulatory subunit of transcription elongation factor	Elongin ABC	ELOA (Elongin A), ELOC (Elongin C), RNA polymerase II	Positive regulation of RNA polymerase II transcript elongation	Elongin complex was originally identified as a positive regulator of RNA polymerase II; Elongin B and C form a regulatory heterodimer that enhances Elongin A activity and increases elongation by suppressing transient pausing. ELOB is the ubiquitin-like subunit within this BC module.	2012	https://doi.org/10.3389/fonc.2012.00010	(okumura2012theroleof pages 1-2, okumura2012theroleof pages 9-10)
Adaptor/core structural subunit in Cullin-RING ligases	VHL/CRL2 (VCB-CR; VHL–ELOB/ELOC–CUL2–RBX1)	VHL, ELOC, CUL2, RBX1, HIF-α, PHD1/2/3	Oxygen sensing; HIF-α ubiquitination and proteasomal degradation	ELOB functions as part of Elongin BC adaptor connecting VHL-box proteins to CUL2. pVHL recognizes prolyl-hydroxylated HIF-α and promotes polyubiquitination/degradation. Review notes that >70% of VHL disease and sporadic clear-cell RCC-associated VHL mutations disrupt the BC-box/Elongin BC interaction; ~57% of sporadic clear-cell RCC harbor inactivating VHL mutations, with ~98% of those showing LOH.	2012	https://doi.org/10.3389/fonc.2012.00010	(okumura2012theroleof pages 2-3)
Structural adaptor helping Cullin selection and ligase assembly	VHL/CRL2 and related ECS ligases	ELOC, VHL-box proteins, CUL2/CUL5, RBX1/RBX2	Assembly/specification of ECS-family E3 ligases	Elongin BC acts as the adaptor connecting Cullin and SOCS/VHL-box substrate receptors. Structural analyses place Elongin C centrally, with ELOB bound as heterodimer partner; Cul2 binding involves hydrophobic/electrostatic contacts and ordering of Elongin C residues 48–57, including insertion of Cul2 Leu3 into an Elongin C pocket.	2016	https://doi.org/10.1186/s13008-016-0020-7	(cai2016thestructureand pages 1-2)
Core component of VHL-type E3 ubiquitin ligase	VHL–Elongin BC E3	VHL, ELOC, CUL2, RBX1, Ubc5-family E2s, HIF transcription factors	Hypoxia-responsive gene control via HIF turnover	VHL-family E3 ligases comprise substrate receptor + Elongins B/C + Cullin + Rbx1. In vitro VHL-dependent HIF ubiquitylation uses Ubc5-family E2 enzymes. This supports ELOB as an essential non-catalytic scaffold/adaptor rather than substrate receptor or enzyme.	2004	https://doi.org/10.1074/jbc.m408018200	(yan2004identificationofelongin pages 2-3)
Adaptor in SOCS-box/CUL5 ligases	CRL5/SOCS-box assemblies	SOCS proteins, ASB proteins, CUL5, RBX2	Cytokine signaling and targeted proteolysis	Elongin BC is the common adaptor recruited by SOCS-box proteins to assemble CUL5-based CRLs. Reviews describe many ASB proteins forming CUL5–RBX2 ubiquitin ligases via Elongin BC, generalizing ELOB’s role across CRL5 family complexes.	2012	https://doi.org/10.3389/fonc.2012.00010	(okumura2012theroleof pages 7-8, okumura2012theroleof pages 8-9)
Adaptor exploited by viral proteins	Viral BC-box/CUL5 or CUL2 ligases	HIV-1 Vif, adenoviral E4orf6, HPV E7, CUL5/CUL2, RBX proteins	Host-pathogen interaction; degradation of host restriction factors	Viral proteins use Elongin BC to build active ubiquitin ligases: HIV-1 Vif recruits a CUL5 ligase that polyubiquitinates APOBEC3F/APOBEC3G; adenoviral E4orf6 forms a CUL5–Elongin BC–RBX1 complex; HPV E7 binds endogenous CUL2. These examples show ELOB’s practical role as a reusable ligase adaptor in infection biology.	2012	https://doi.org/10.3389/fonc.2012.00010	(okumura2012theroleof pages 9-10, okumura2012theroleof pages 7-8)
Component of recently defined innate-immunity E3 ligase	CRL5–SPSB3–ELOBC	SPSB3, ELOC, CUL5, RBX2, NEDD8, UBE2F, ARIH2, cGAS	Nuclear cGAS turnover; innate immune homeostasis	A 2024 Nature study identified all core CRL5 components, including TCEB2/ELOB, as top hits in an RNAi screen of 972 UPS genes regulating nuclear cGAS–GFP abundance; hits were defined as >3 s.d. increases in reporter signal. SPSB3 binds ELOBC through its SOCS box, linking substrate recognition to the CUL5–RBX2 catalytic core.	2024	https://doi.org/10.1038/s41586-024-07112-w	(xu2024thecrl5–spsb3ubiquitin pages 2-3, xu2024thecrl5–spsb3ubiquitin media d33342da)
Structural scaffold enabling substrate recognition in CRL5	CRL5–SPSB3–ELOBC	SPSB3, cGAS, nucleosome core particle, CUL5, ARIH2	Ubiquitylation of nucleosome-bound nuclear cGAS	Biochemical and structural work showed SPSB3 core is stabilized by ELOBC, and cryo-EM resolved cGAS–nucleosome–SPSB3–ELOBC assemblies; a 3.5 Å reconstruction supported modelling of neddylated ELOBC–CRL5–ARIH2 positioned to ubiquitinate cGAS Lys427/Lys428.	2024	https://doi.org/10.1038/s41586-024-07112-w	(xu2024thecrl5–spsb3ubiquitin pages 3-4, xu2024thecrl5–spsb3ubiquitin pages 4-5, xu2024thecrl5–spsb3ubiquitin media d33342da)
Quantitatively characterized adaptor-containing receptor complex	SPSB3–ELOBC subcomplex within CRL5	SPSB3, cGAS	Degron recognition preceding ubiquitination	Bio-layer interferometry showed WT cGAS binds SPSB3–ELOBC with submicromolar affinity (Kd = 352 nM). A conserved cGAS NN degron (Asn513/Asn514) was required: single substitutions abolished detectable binding and prevented in vitro ubiquitylation, demonstrating how ELOB-containing ELOBC supports selective substrate engagement.	2024	https://doi.org/10.1038/s41586-024-07112-w	(xu2024thecrl5–spsb3ubiquitin pages 5-6, xu2024thecrl5–spsb3ubiquitin media d33342da)
ELOB-containing ligase linked to site-specific ubiquitination outcome	CRL5–SPSB3–ELOBC	SPSB3, CUL5, ELOC, ARIH2, cGAS	Proteasomal degradation of nuclear cGAS	Mass spectrometry identified five ubiquitinated sites in the cGAS catalytic core, with human Lys427/Lys428 as the dominant pair; mutation of this pair largely abolished ubiquitylation and nuclear degradation. In cells, ubiquitin chains were Lys48-linked and loss of SPSB3/CUL5 or neddylation inhibition (MLN4924) stabilized nuclear cGAS.	2024	https://doi.org/10.1038/s41586-024-07112-w	(xu2024thecrl5–spsb3ubiquitin pages 3-4, xu2024thecrl5–spsb3ubiquitin pages 2-3)

Table: This table summarizes the validated functions of human ELOB/TCEB2 across transcription elongation and Cullin-RING ubiquitin ligase pathways, with emphasis on key complexes, partners, mechanisms, and quantitative findings. It is useful as a compact evidence map linking classical ELOB biology to recent 2024 mechanistic studies.

8. Limitations and evidence gaps

Direct, ELOB-specific subcellular localization statements (e.g., nucleus vs cytosol distributions of ELOB itself) were not explicitly extractable from the retrieved text snippets; localization inference is mainly through complex functions (Pol II elongation implies nuclear involvement; CRL5–SPSB3 is explicitly nuclear for cGAS turnover). (okumura2012theroleof pages 1-2, xu2024thecrl5–spsb3ubiquitin pages 2-3)
The report prioritizes mechanistically detailed and recent sources; however, some classical foundational structural work on Elongin BC/VHL complexes exists outside the 2023–2024 window and was not required to establish the key roles summarized here.

References

(okumura2012theroleof pages 1-2): Fumihiko Okumura, Mariko Matsuzaki, Kunio Nakatsukasa, and Takumi Kamura. The role of elongin bc-containing ubiquitin ligases. Frontiers in Oncology, Jan 2012. URL: https://doi.org/10.3389/fonc.2012.00010, doi:10.3389/fonc.2012.00010. This article has 114 citations.
(xu2024thecrl5–spsb3ubiquitin pages 2-3): Pengbiao Xu, Ying Liu, Chong Liu, Baptiste Guey, Lingyun Li, Pauline Melenec, Jonathan Ricci, and Andrea Ablasser. The crl5–spsb3 ubiquitin ligase targets nuclear cgas for degradation. Nature, 627:873-879, Feb 2024. URL: https://doi.org/10.1038/s41586-024-07112-w, doi:10.1038/s41586-024-07112-w. This article has 65 citations and is from a highest quality peer-reviewed journal.
(cai2016thestructureand pages 1-2): Weijia Cai and Haifeng Yang. The structure and regulation of cullin 2 based e3 ubiquitin ligases and their biological functions. Cell Division, Mar 2016. URL: https://doi.org/10.1186/s13008-016-0020-7, doi:10.1186/s13008-016-0020-7. This article has 107 citations and is from a peer-reviewed journal.
(okumura2012theroleof pages 2-3): Fumihiko Okumura, Mariko Matsuzaki, Kunio Nakatsukasa, and Takumi Kamura. The role of elongin bc-containing ubiquitin ligases. Frontiers in Oncology, Jan 2012. URL: https://doi.org/10.3389/fonc.2012.00010, doi:10.3389/fonc.2012.00010. This article has 114 citations.
(yan2004identificationofelongin pages 2-3): Qin Yan, Takumi Kamura, Yong Cai, Jingji Jin, Mircea Ivan, Arcady Mushegian, Ronald C. Conaway, and Joan Weliky Conaway. Identification of elongin c and skp1 sequences that determine cullin selection*. Journal of Biological Chemistry, 279:43019-43026, Oct 2004. URL: https://doi.org/10.1074/jbc.m408018200, doi:10.1074/jbc.m408018200. This article has 16 citations and is from a domain leading peer-reviewed journal.
(okumura2012theroleof pages 7-8): Fumihiko Okumura, Mariko Matsuzaki, Kunio Nakatsukasa, and Takumi Kamura. The role of elongin bc-containing ubiquitin ligases. Frontiers in Oncology, Jan 2012. URL: https://doi.org/10.3389/fonc.2012.00010, doi:10.3389/fonc.2012.00010. This article has 114 citations.
(okumura2012theroleof pages 8-9): Fumihiko Okumura, Mariko Matsuzaki, Kunio Nakatsukasa, and Takumi Kamura. The role of elongin bc-containing ubiquitin ligases. Frontiers in Oncology, Jan 2012. URL: https://doi.org/10.3389/fonc.2012.00010, doi:10.3389/fonc.2012.00010. This article has 114 citations.
(xu2024thecrl5–spsb3ubiquitin pages 3-4): Pengbiao Xu, Ying Liu, Chong Liu, Baptiste Guey, Lingyun Li, Pauline Melenec, Jonathan Ricci, and Andrea Ablasser. The crl5–spsb3 ubiquitin ligase targets nuclear cgas for degradation. Nature, 627:873-879, Feb 2024. URL: https://doi.org/10.1038/s41586-024-07112-w, doi:10.1038/s41586-024-07112-w. This article has 65 citations and is from a highest quality peer-reviewed journal.
(xu2024thecrl5–spsb3ubiquitin pages 5-6): Pengbiao Xu, Ying Liu, Chong Liu, Baptiste Guey, Lingyun Li, Pauline Melenec, Jonathan Ricci, and Andrea Ablasser. The crl5–spsb3 ubiquitin ligase targets nuclear cgas for degradation. Nature, 627:873-879, Feb 2024. URL: https://doi.org/10.1038/s41586-024-07112-w, doi:10.1038/s41586-024-07112-w. This article has 65 citations and is from a highest quality peer-reviewed journal.
(xu2024thecrl5–spsb3ubiquitin media d33342da): Pengbiao Xu, Ying Liu, Chong Liu, Baptiste Guey, Lingyun Li, Pauline Melenec, Jonathan Ricci, and Andrea Ablasser. The crl5–spsb3 ubiquitin ligase targets nuclear cgas for degradation. Nature, 627:873-879, Feb 2024. URL: https://doi.org/10.1038/s41586-024-07112-w, doi:10.1038/s41586-024-07112-w. This article has 65 citations and is from a highest quality peer-reviewed journal.
(xu2024thecrl5–spsb3ubiquitin media abdf9a09): Pengbiao Xu, Ying Liu, Chong Liu, Baptiste Guey, Lingyun Li, Pauline Melenec, Jonathan Ricci, and Andrea Ablasser. The crl5–spsb3 ubiquitin ligase targets nuclear cgas for degradation. Nature, 627:873-879, Feb 2024. URL: https://doi.org/10.1038/s41586-024-07112-w, doi:10.1038/s41586-024-07112-w. This article has 65 citations and is from a highest quality peer-reviewed journal.
(xu2024thecrl5–spsb3ubiquitin media 84ea615a): Pengbiao Xu, Ying Liu, Chong Liu, Baptiste Guey, Lingyun Li, Pauline Melenec, Jonathan Ricci, and Andrea Ablasser. The crl5–spsb3 ubiquitin ligase targets nuclear cgas for degradation. Nature, 627:873-879, Feb 2024. URL: https://doi.org/10.1038/s41586-024-07112-w, doi:10.1038/s41586-024-07112-w. This article has 65 citations and is from a highest quality peer-reviewed journal.
(hanzl2023functionale3ligase pages 1-4): Alexander Hanzl, Ryan Casement, Hana Imrichova, Scott J. Hughes, Eleonora Barone, Andrea Testa, Sophie Bauer, Jane Wright, Matthias Brand, Alessio Ciulli, and Georg E. Winter. Functional e3 ligase hotspots and resistance mechanisms to small-molecule degraders. Nature chemical biology, 19:323-333, Nov 2023. URL: https://doi.org/10.1038/s41589-022-01177-2, doi:10.1038/s41589-022-01177-2. This article has 124 citations and is from a highest quality peer-reviewed journal.
(bouvier2024breakingbadproteins—discovery pages 7-9): Corentin Bouvier, Rachel Lawrence, Francesca Cavallo, Wendy Xolalpa, Allan Jordan, Roland Hjerpe, and Manuel S. Rodriguez. Breaking bad proteins—discovery approaches and the road to clinic for degraders. Cells, 13:578, Mar 2024. URL: https://doi.org/10.3390/cells13070578, doi:10.3390/cells13070578. This article has 24 citations.
(lin2024diversityofstructure pages 3-5): Calvin P. Lin and Elizabeth A. Komives. Diversity of structure and function in cullin e3 ligases. Current Opinion in Structural Biology, 88:102879, Oct 2024. URL: https://doi.org/10.1016/j.sbi.2024.102879, doi:10.1016/j.sbi.2024.102879. This article has 14 citations and is from a peer-reviewed journal.
(xu2024thecrl5–spsb3ubiquitin pages 4-5): Pengbiao Xu, Ying Liu, Chong Liu, Baptiste Guey, Lingyun Li, Pauline Melenec, Jonathan Ricci, and Andrea Ablasser. The crl5–spsb3 ubiquitin ligase targets nuclear cgas for degradation. Nature, 627:873-879, Feb 2024. URL: https://doi.org/10.1038/s41586-024-07112-w, doi:10.1038/s41586-024-07112-w. This article has 65 citations and is from a highest quality peer-reviewed journal.
(okumura2012theroleof pages 9-10): Fumihiko Okumura, Mariko Matsuzaki, Kunio Nakatsukasa, and Takumi Kamura. The role of elongin bc-containing ubiquitin ligases. Frontiers in Oncology, Jan 2012. URL: https://doi.org/10.3389/fonc.2012.00010, doi:10.3389/fonc.2012.00010. This article has 114 citations.

Citations

okumura2012theroleof pages 1-2
yan2004identificationofelongin pages 2-3
cai2016thestructureand pages 1-2
lin2024diversityofstructure pages 3-5
okumura2012theroleof pages 7-8
okumura2012theroleof pages 2-3
okumura2012theroleof pages 8-9
okumura2012theroleof pages 9-10
https://doi.org/10.3389/fonc.2012.00010
https://doi.org/10.1074/jbc.m408018200
https://doi.org/10.1038/s41586-024-07112-w
https://doi.org/10.1186/s13008-016-0020-7
https://doi.org/10.1038/s41589-022-01177-2
https://doi.org/10.3390/cells13070578
https://doi.org/10.1016/j.sbi.2024.102879
https://doi.org/10.3389/fonc.2012.00010,
https://doi.org/10.1038/s41586-024-07112-w,
https://doi.org/10.1186/s13008-016-0020-7,
https://doi.org/10.1074/jbc.m408018200,
https://doi.org/10.1038/s41589-022-01177-2,
https://doi.org/10.3390/cells13070578,
https://doi.org/10.1016/j.sbi.2024.102879,

📄 View Raw YAML

id: Q15370
gene_symbol: ELOB
product_type: PROTEIN
status: COMPLETE
taxon:
  id: NCBITaxon:9606
  label: Homo sapiens
description: ELOB encodes Elongin B, a small ubiquitin-like, non-catalytic regulatory/adaptor
  subunit of the Elongin BC module. Together with ELOC, it supports Elongin transcription
  elongation complexes and acts as a reusable adaptor module in Cul2/Cul5 Cullin-RING E3 ubiquitin
  ligases, including VHL, SOCS-box, viral, and recent CRL5-SPSB3 nuclear cGAS turnover contexts.
alternative_products:
- name: '1'
  id: Q15370-1
- name: '2'
  id: Q15370-2
  sequence_note: VSP_045784
existing_annotations:
- term:
    id: GO:0070449
    label: elongin complex
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: Correct. ELOB is the ubiquitin-like Elongin B subunit of the Elongin BC regulatory
      module and Elongin ABC complex.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    supported_by:
    - reference_id: file:human/ELOB/ELOB-deep-research-falcon.md
      supporting_text: "for ELOB itself, the best-supported \u201Cprimary function\u201D in\
        \ mechanistic terms is **non-enzymatic**\u2014it serves as a **ubiquitin-like adaptor/regulatory\
        \ subunit** within Elongin BC"
- term:
    id: GO:0030891
    label: VCB complex
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: Correct. ELOB/ELOC form the Elongin BC module in the VHL-containing CRL2/VCB
      ubiquitin ligase complex.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    supported_by:
    - reference_id: file:human/ELOB/ELOB-deep-research-falcon.md
      supporting_text: "In the **CRL2^VHL** ligase, the substrate receptor **pVHL** binds\
        \ Elongin BC and engages **CUL2\u2013RBX1** to ubiquitinate substrates"
- term:
    id: GO:0000151
    label: ubiquitin ligase complex
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: Correct but broad. ELOB is a non-catalytic adaptor/regulatory subunit in more
      specific Cul2/Cul5 ubiquitin ligase complexes.
    action: MARK_AS_OVER_ANNOTATED
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: More specific CRL2/CRL5 and VCB complex annotations capture the supported complex
      membership.
- term:
    id: GO:0005634
    label: nucleus
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  review:
    summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase
      II elongation and nuclear cGAS turnover contexts.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005737
    label: cytoplasm
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: Correct but less specific than cytosol/nucleoplasm annotations for ELOB-containing
      complexes.
    action: MARK_AS_OVER_ANNOTATED
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Use cytosol and nucleoplasm where available rather than generic cytoplasm.
- term:
    id: GO:0006368
    label: transcription elongation by RNA polymerase II
  evidence_type: IEA
  original_reference_id: GO_REF:0000002
  review:
    summary: Accepted. ELOB is a regulatory Elongin subunit in the Elongin ABC transcription
      elongation factor that promotes RNA polymerase II elongation.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    supported_by:
    - reference_id: file:human/ELOB/ELOB-deep-research-falcon.md
      supporting_text: '**Elongin** was originally identified as a positive regulator of **RNA
        polymerase II** transcription elongation.'
- term:
    id: GO:0030891
    label: VCB complex
  evidence_type: IEA
  original_reference_id: GO_REF:0000002
  review:
    summary: Correct. ELOB/ELOC form the Elongin BC module in the VHL-containing CRL2/VCB
      ubiquitin ligase complex.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    supported_by:
    - reference_id: file:human/ELOB/ELOB-deep-research-falcon.md
      supporting_text: "In the **CRL2^VHL** ligase, the substrate receptor **pVHL** binds\
        \ Elongin BC and engages **CUL2\u2013RBX1** to ubiquitinate substrates"
- term:
    id: GO:0070449
    label: elongin complex
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  review:
    summary: Correct. ELOB is the ubiquitin-like Elongin B subunit of the Elongin BC regulatory
      module and Elongin ABC complex.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    supported_by:
    - reference_id: file:human/ELOB/ELOB-deep-research-falcon.md
      supporting_text: "for ELOB itself, the best-supported \u201Cprimary function\u201D in\
        \ mechanistic terms is **non-enzymatic**\u2014it serves as a **ubiquitin-like adaptor/regulatory\
        \ subunit** within Elongin BC"
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:10205047
  review:
    summary: Protein binding is too generic for ELOB. The informative annotation is Elongin
      BC adaptor/regulatory subunit activity in Elongin and CRL complexes.
    action: REMOVE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context
      annotations.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:12004076
  review:
    summary: Protein binding is too generic for ELOB. The informative annotation is Elongin
      BC adaptor/regulatory subunit activity in Elongin and CRL complexes.
    action: REMOVE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context
      annotations.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:12050673
  review:
    summary: Protein binding is too generic for ELOB. The informative annotation is Elongin
      BC adaptor/regulatory subunit activity in Elongin and CRL complexes.
    action: REMOVE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context
      annotations.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:12149480
  review:
    summary: Protein binding is too generic for ELOB. The informative annotation is Elongin
      BC adaptor/regulatory subunit activity in Elongin and CRL complexes.
    action: REMOVE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context
      annotations.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:15601820
  review:
    summary: Protein binding is too generic for ELOB. The informative annotation is Elongin
      BC adaptor/regulatory subunit activity in Elongin and CRL complexes.
    action: REMOVE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context
      annotations.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:16189514
  review:
    summary: Protein binding is too generic for ELOB. The informative annotation is Elongin
      BC adaptor/regulatory subunit activity in Elongin and CRL complexes.
    action: REMOVE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context
      annotations.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:18323857
  review:
    summary: Protein binding is too generic for ELOB. The informative annotation is Elongin
      BC adaptor/regulatory subunit activity in Elongin and CRL complexes.
    action: REMOVE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context
      annotations.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:20211142
  review:
    summary: Protein binding is too generic for ELOB. The informative annotation is Elongin
      BC adaptor/regulatory subunit activity in Elongin and CRL complexes.
    action: REMOVE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context
      annotations.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:20871634
  review:
    summary: Protein binding is too generic for ELOB. The informative annotation is Elongin
      BC adaptor/regulatory subunit activity in Elongin and CRL complexes.
    action: REMOVE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context
      annotations.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:21516116
  review:
    summary: Protein binding is too generic for ELOB. The informative annotation is Elongin
      BC adaptor/regulatory subunit activity in Elongin and CRL complexes.
    action: REMOVE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context
      annotations.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:22190034
  review:
    summary: Protein binding is too generic for ELOB. The informative annotation is Elongin
      BC adaptor/regulatory subunit activity in Elongin and CRL complexes.
    action: REMOVE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context
      annotations.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:25416956
  review:
    summary: Protein binding is too generic for ELOB. The informative annotation is Elongin
      BC adaptor/regulatory subunit activity in Elongin and CRL complexes.
    action: REMOVE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context
      annotations.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:27517496
  review:
    summary: Protein binding is too generic for ELOB. The informative annotation is Elongin
      BC adaptor/regulatory subunit activity in Elongin and CRL complexes.
    action: REMOVE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context
      annotations.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:30026228
  review:
    summary: Protein binding is too generic for ELOB. The informative annotation is Elongin
      BC adaptor/regulatory subunit activity in Elongin and CRL complexes.
    action: REMOVE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context
      annotations.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:32296183
  review:
    summary: Protein binding is too generic for ELOB. The informative annotation is Elongin
      BC adaptor/regulatory subunit activity in Elongin and CRL complexes.
    action: REMOVE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context
      annotations.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:33961781
  review:
    summary: Protein binding is too generic for ELOB. The informative annotation is Elongin
      BC adaptor/regulatory subunit activity in Elongin and CRL complexes.
    action: REMOVE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context
      annotations.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:35271311
  review:
    summary: Protein binding is too generic for ELOB. The informative annotation is Elongin
      BC adaptor/regulatory subunit activity in Elongin and CRL complexes.
    action: REMOVE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context
      annotations.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:40205054
  review:
    summary: Protein binding is too generic for ELOB. The informative annotation is Elongin
      BC adaptor/regulatory subunit activity in Elongin and CRL complexes.
    action: REMOVE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context
      annotations.
- term:
    id: GO:0016567
    label: protein ubiquitination
  evidence_type: IEA
  original_reference_id: GO_REF:0000041
  review:
    summary: Supported as a broad process enabled by ELOB-containing CRL2/CRL5 ligases,
      though ELOB itself is a non-catalytic Elongin BC adaptor/regulatory subunit,
      not a substrate receptor or ubiquitin-transfer enzyme.
    action: ACCEPT
    reason: Keep the process annotation because ELOB is a direct required module in
      CRL ubiquitination complexes. Do not add GO:1990756 for ELOB itself, because
      substrate recognition is supplied by SOCS-box, VHL-box, or related substrate
      receptor proteins rather than by ELOB.
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    supported_by:
    - reference_id: file:human/ELOB/ELOB-deep-research-falcon.md
      supporting_text: Elongin BC acts as an **adaptor** that couples substrate-recognition
        proteins bearing a **VHL box** or **SOCS box** to a Cullin scaffold
- term:
    id: GO:0031466
    label: Cul5-RING ubiquitin ligase complex
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the
      Elongin BC adaptor module used by SOCS-box substrate receptors.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md

- term:
    id: GO:0031466
    label: Cul5-RING ubiquitin ligase complex
  evidence_type: IDA
  original_reference_id: PMID:40963025
  review:
    summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the
      Elongin BC adaptor module used by SOCS-box substrate receptors.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md

- term:
    id: GO:0000122
    label: negative regulation of transcription by RNA polymerase II
  evidence_type: NAS
  original_reference_id: PMID:28591624
  review:
    summary: Context-specific transcriptional regulation annotation. ELOB core biology is
      Elongin/CRL adaptor function rather than a dedicated transcriptional repressor.
    action: KEEP_AS_NON_CORE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Non-core transcription regulatory consequence of Elongin-containing complexes.
- term:
    id: GO:0043161
    label: proteasome-mediated ubiquitin-dependent protein catabolic process
  evidence_type: NAS
  original_reference_id: PMID:31273098
  review:
    summary: Supported as a broad consequence of ELOB-containing Cullin-RING ligase activity,
      but less specific than complex/adaptor annotations.
    action: KEEP_AS_NON_CORE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: ELOB is a non-catalytic adaptor/regulatory subunit within the ubiquitin ligase
      machinery.
- term:
    id: GO:0140627
    label: ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
  evidence_type: NAS
  original_reference_id: PMID:15601820
  review:
    summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin
      ligase complexes, but not the general core molecular role of ELOB.
    action: KEEP_AS_NON_CORE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex
      function.
- term:
    id: GO:0140627
    label: ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
  evidence_type: NAS
  original_reference_id: PMID:21822215
  review:
    summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin
      ligase complexes, but not the general core molecular role of ELOB.
    action: KEEP_AS_NON_CORE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex
      function.
- term:
    id: GO:0140627
    label: ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
  evidence_type: NAS
  original_reference_id: PMID:22649776
  review:
    summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin
      ligase complexes, but not the general core molecular role of ELOB.
    action: KEEP_AS_NON_CORE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex
      function.
- term:
    id: GO:0140627
    label: ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
  evidence_type: NAS
  original_reference_id: PMID:29779948
  review:
    summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin
      ligase complexes, but not the general core molecular role of ELOB.
    action: KEEP_AS_NON_CORE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex
      function.
- term:
    id: GO:0140627
    label: ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
  evidence_type: NAS
  original_reference_id: PMID:33398168
  review:
    summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin
      ligase complexes, but not the general core molecular role of ELOB.
    action: KEEP_AS_NON_CORE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex
      function.
- term:
    id: GO:0140627
    label: ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
  evidence_type: NAS
  original_reference_id: PMID:34743205
  review:
    summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin
      ligase complexes, but not the general core molecular role of ELOB.
    action: KEEP_AS_NON_CORE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex
      function.
- term:
    id: GO:1990116
    label: ribosome-associated ubiquitin-dependent protein catabolic process
  evidence_type: NAS
  original_reference_id: PMID:33909987
  review:
    summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin
      ligase complexes, but not the general core molecular role of ELOB.
    action: KEEP_AS_NON_CORE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex
      function.
- term:
    id: GO:2000104
    label: negative regulation of DNA-templated DNA replication
  evidence_type: NAS
  original_reference_id: PMID:22649776
  review:
    summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin
      ligase complexes, but not the general core molecular role of ELOB.
    action: KEEP_AS_NON_CORE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex
      function.
- term:
    id: GO:0031466
    label: Cul5-RING ubiquitin ligase complex
  evidence_type: IDA
  original_reference_id: PMID:39039081
  review:
    summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the
      Elongin BC adaptor module used by SOCS-box substrate receptors.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md

- term:
    id: GO:0031466
    label: Cul5-RING ubiquitin ligase complex
  evidence_type: IDA
  original_reference_id: PMID:40440427
  review:
    summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the
      Elongin BC adaptor module used by SOCS-box substrate receptors.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md

- term:
    id: GO:0031462
    label: Cul2-RING ubiquitin ligase complex
  evidence_type: IDA
  original_reference_id: PMID:35978186
  review:
    summary: Correct. ELOB participates in Cul2-RING ubiquitin ligase assemblies such as CRL2-VHL
      through the Elongin BC adaptor module.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    supported_by:
    - reference_id: file:human/ELOB/ELOB-deep-research-falcon.md
      supporting_text: "In the **CRL2^VHL** ligase, the substrate receptor **pVHL** binds\
        \ Elongin BC and engages **CUL2\u2013RBX1** to ubiquitinate substrates"
- term:
    id: GO:0031462
    label: Cul2-RING ubiquitin ligase complex
  evidence_type: IDA
  original_reference_id: PMID:37844242
  review:
    summary: Correct. ELOB participates in Cul2-RING ubiquitin ligase assemblies such as CRL2-VHL
      through the Elongin BC adaptor module.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    supported_by:
    - reference_id: file:human/ELOB/ELOB-deep-research-falcon.md
      supporting_text: "In the **CRL2^VHL** ligase, the substrate receptor **pVHL** binds\
        \ Elongin BC and engages **CUL2\u2013RBX1** to ubiquitinate substrates"
- term:
    id: GO:0140627
    label: ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
  evidence_type: IDA
  original_reference_id: PMID:37844242
  review:
    summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin
      ligase complexes, but not the general core molecular role of ELOB.
    action: KEEP_AS_NON_CORE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex
      function.
- term:
    id: GO:0031466
    label: Cul5-RING ubiquitin ligase complex
  evidence_type: IDA
  original_reference_id: PMID:35512830
  review:
    summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the
      Elongin BC adaptor module used by SOCS-box substrate receptors.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md

- term:
    id: GO:0031466
    label: Cul5-RING ubiquitin ligase complex
  evidence_type: IDA
  original_reference_id: PMID:37816714
  review:
    summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the
      Elongin BC adaptor module used by SOCS-box substrate receptors.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md

- term:
    id: GO:0031466
    label: Cul5-RING ubiquitin ligase complex
  evidence_type: IDA
  original_reference_id: PMID:34857742
  review:
    summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the
      Elongin BC adaptor module used by SOCS-box substrate receptors.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md

- term:
    id: GO:0031466
    label: Cul5-RING ubiquitin ligase complex
  evidence_type: IDA
  original_reference_id: PMID:21980433
  review:
    summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the
      Elongin BC adaptor module used by SOCS-box substrate receptors.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md

- term:
    id: GO:0031466
    label: Cul5-RING ubiquitin ligase complex
  evidence_type: IDA
  original_reference_id: PMID:25505247
  review:
    summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the
      Elongin BC adaptor module used by SOCS-box substrate receptors.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md

- term:
    id: GO:0031466
    label: Cul5-RING ubiquitin ligase complex
  evidence_type: IDA
  original_reference_id: PMID:38418882
  review:
    summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the
      Elongin BC adaptor module used by SOCS-box substrate receptors.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md

- term:
    id: GO:0031466
    label: Cul5-RING ubiquitin ligase complex
  evidence_type: IDA
  original_reference_id: PMID:31387940
  review:
    summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the
      Elongin BC adaptor module used by SOCS-box substrate receptors.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md

- term:
    id: GO:0031466
    label: Cul5-RING ubiquitin ligase complex
  evidence_type: IDA
  original_reference_id: PMID:32513959
  review:
    summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the
      Elongin BC adaptor module used by SOCS-box substrate receptors.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md

- term:
    id: GO:0031466
    label: Cul5-RING ubiquitin ligase complex
  evidence_type: IDA
  original_reference_id: PMID:23837592
  review:
    summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the
      Elongin BC adaptor module used by SOCS-box substrate receptors.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md

- term:
    id: GO:0031466
    label: Cul5-RING ubiquitin ligase complex
  evidence_type: IDA
  original_reference_id: PMID:33268465
  review:
    summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the
      Elongin BC adaptor module used by SOCS-box substrate receptors.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md

- term:
    id: GO:0140958
    label: target-directed miRNA degradation
  evidence_type: IMP
  original_reference_id: PMID:33184237
  review:
    summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin
      ligase complexes, but not the general core molecular role of ELOB.
    action: KEEP_AS_NON_CORE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex
      function.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:22510880
  review:
    summary: Protein binding is too generic for ELOB. The informative annotation is Elongin
      BC adaptor/regulatory subunit activity in Elongin and CRL complexes.
    action: REMOVE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context
      annotations.
- term:
    id: GO:0001222
    label: transcription corepressor binding
  evidence_type: IPI
  original_reference_id: PMID:7660122
  review:
    summary: Supported as a binding/regulatory-context annotation but secondary to ELOB's
      broader Elongin BC adaptor role.
    action: KEEP_AS_NON_CORE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Non-core interaction-specific molecular function.
- term:
    id: GO:0006367
    label: transcription initiation at RNA polymerase II promoter
  evidence_type: IDA
  original_reference_id: PMID:7660122
  review:
    summary: Remove. The cited PMID:7660122 paper concerns VHL inhibition of Elongin-dependent
      RNA polymerase II transcription elongation, not transcription initiation.
    action: REMOVE
    reason: 'Evidence-term mismatch: PMID:7660122 supports transcription elongation biology,
      not GO:0006367 transcription initiation at RNA polymerase II promoter.'
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md

- term:
    id: GO:0070449
    label: elongin complex
  evidence_type: IDA
  original_reference_id: PMID:7660122
  review:
    summary: Correct. ELOB is the ubiquitin-like Elongin B subunit of the Elongin BC regulatory
      module and Elongin ABC complex.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    supported_by:
    - reference_id: file:human/ELOB/ELOB-deep-research-falcon.md
      supporting_text: "for ELOB itself, the best-supported \u201Cprimary function\u201D in\
        \ mechanistic terms is **non-enzymatic**\u2014it serves as a **ubiquitin-like adaptor/regulatory\
        \ subunit** within Elongin BC"
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:21119685
  review:
    summary: Protein binding is too generic for ELOB. The informative annotation is Elongin
      BC adaptor/regulatory subunit activity in Elongin and CRL complexes.
    action: REMOVE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context
      annotations.
- term:
    id: GO:0032436
    label: positive regulation of proteasomal ubiquitin-dependent protein catabolic process
  evidence_type: ISS
  original_reference_id: GO_REF:0000024
  review:
    summary: Supported as a broad consequence of ELOB-containing Cullin-RING ligase activity,
      but less specific than complex/adaptor annotations.
    action: KEEP_AS_NON_CORE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: ELOB is a non-catalytic adaptor/regulatory subunit within the ubiquitin ligase
      machinery.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:23401859
  review:
    summary: Protein binding is too generic for ELOB. The informative annotation is Elongin
      BC adaptor/regulatory subunit activity in Elongin and CRL complexes.
    action: REMOVE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context
      annotations.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:26906416
  review:
    summary: Protein binding is too generic for ELOB. The informative annotation is Elongin
      BC adaptor/regulatory subunit activity in Elongin and CRL complexes.
    action: REMOVE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context
      annotations.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-1234159
  review:
    summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL
      and viral/SOCS-box ligase assemblies.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-1234163
  review:
    summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL
      and viral/SOCS-box ligase assemblies.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-1234173
  review:
    summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL
      and viral/SOCS-box ligase assemblies.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-1234175
  review:
    summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL
      and viral/SOCS-box ligase assemblies.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-1234177
  review:
    summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL
      and viral/SOCS-box ligase assemblies.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-1234183
  review:
    summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL
      and viral/SOCS-box ligase assemblies.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-180540
  review:
    summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL
      and viral/SOCS-box ligase assemblies.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-180555
  review:
    summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL
      and viral/SOCS-box ligase assemblies.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-180603
  review:
    summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL
      and viral/SOCS-box ligase assemblies.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-8952039
  review:
    summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL
      and viral/SOCS-box ligase assemblies.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-8952044
  review:
    summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL
      and viral/SOCS-box ligase assemblies.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-8952625
  review:
    summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL
      and viral/SOCS-box ligase assemblies.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-8952626
  review:
    summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL
      and viral/SOCS-box ligase assemblies.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-8955241
  review:
    summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL
      and viral/SOCS-box ligase assemblies.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-8955289
  review:
    summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL
      and viral/SOCS-box ligase assemblies.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-8956040
  review:
    summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL
      and viral/SOCS-box ligase assemblies.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-8956099
  review:
    summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL
      and viral/SOCS-box ligase assemblies.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-8956103
  review:
    summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL
      and viral/SOCS-box ligase assemblies.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-8956106
  review:
    summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL
      and viral/SOCS-box ligase assemblies.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9705738
  review:
    summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL
      and viral/SOCS-box ligase assemblies.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9755303
  review:
    summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL
      and viral/SOCS-box ligase assemblies.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9833107
  review:
    summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL
      and viral/SOCS-box ligase assemblies.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9833155
  review:
    summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL
      and viral/SOCS-box ligase assemblies.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9954721
  review:
    summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL
      and viral/SOCS-box ligase assemblies.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9954723
  review:
    summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL
      and viral/SOCS-box ligase assemblies.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005654
    label: nucleoplasm
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-112379
  review:
    summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase
      II elongation and nuclear cGAS turnover contexts.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005654
    label: nucleoplasm
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-112385
  review:
    summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase
      II elongation and nuclear cGAS turnover contexts.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005654
    label: nucleoplasm
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-112392
  review:
    summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase
      II elongation and nuclear cGAS turnover contexts.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005654
    label: nucleoplasm
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-112395
  review:
    summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase
      II elongation and nuclear cGAS turnover contexts.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005654
    label: nucleoplasm
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-112396
  review:
    summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase
      II elongation and nuclear cGAS turnover contexts.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005654
    label: nucleoplasm
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-112435
  review:
    summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase
      II elongation and nuclear cGAS turnover contexts.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005654
    label: nucleoplasm
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-112436
  review:
    summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase
      II elongation and nuclear cGAS turnover contexts.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005654
    label: nucleoplasm
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-113411
  review:
    summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase
      II elongation and nuclear cGAS turnover contexts.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005654
    label: nucleoplasm
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-113412
  review:
    summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase
      II elongation and nuclear cGAS turnover contexts.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005654
    label: nucleoplasm
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-113413
  review:
    summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase
      II elongation and nuclear cGAS turnover contexts.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005654
    label: nucleoplasm
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-113414
  review:
    summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase
      II elongation and nuclear cGAS turnover contexts.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005654
    label: nucleoplasm
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-113429
  review:
    summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase
      II elongation and nuclear cGAS turnover contexts.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005654
    label: nucleoplasm
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-1234169
  review:
    summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase
      II elongation and nuclear cGAS turnover contexts.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005654
    label: nucleoplasm
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-1234172
  review:
    summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase
      II elongation and nuclear cGAS turnover contexts.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005654
    label: nucleoplasm
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-1234175
  review:
    summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase
      II elongation and nuclear cGAS turnover contexts.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005654
    label: nucleoplasm
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-6797606
  review:
    summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase
      II elongation and nuclear cGAS turnover contexts.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0005654
    label: nucleoplasm
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-6797616
  review:
    summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase
      II elongation and nuclear cGAS turnover contexts.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
- term:
    id: GO:0031625
    label: ubiquitin protein ligase binding
  evidence_type: IDA
  original_reference_id: PMID:17636018
  review:
    summary: Accepted. ELOB binds and stabilizes ubiquitin ligase assemblies as part of the
      Elongin BC adaptor module.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    supported_by:
    - reference_id: file:human/ELOB/ELOB-deep-research-falcon.md
      supporting_text: Elongin BC acts as an **adaptor** that couples substrate-recognition
        proteins bearing a **VHL box** or **SOCS box** to a Cullin scaffold
- term:
    id: GO:0031462
    label: Cul2-RING ubiquitin ligase complex
  evidence_type: IDA
  original_reference_id: PMID:17636018
  review:
    summary: Correct. ELOB participates in Cul2-RING ubiquitin ligase assemblies such as CRL2-VHL
      through the Elongin BC adaptor module.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    supported_by:
    - reference_id: file:human/ELOB/ELOB-deep-research-falcon.md
      supporting_text: "In the **CRL2^VHL** ligase, the substrate receptor **pVHL** binds\
        \ Elongin BC and engages **CUL2\u2013RBX1** to ubiquitinate substrates"
- term:
    id: GO:0031466
    label: Cul5-RING ubiquitin ligase complex
  evidence_type: IDA
  original_reference_id: PMID:17636018
  review:
    summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the
      Elongin BC adaptor module used by SOCS-box substrate receptors.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md

- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:15590694
  review:
    summary: Protein binding is too generic for ELOB. The informative annotation is Elongin
      BC adaptor/regulatory subunit activity in Elongin and CRL complexes.
    action: REMOVE
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context
      annotations.
- term:
    id: GO:0006368
    label: transcription elongation by RNA polymerase II
  evidence_type: TAS
  original_reference_id: PMID:7660129
  review:
    summary: Accepted. ELOB is a regulatory Elongin subunit in the Elongin ABC transcription
      elongation factor that promotes RNA polymerase II elongation.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    supported_by:
    - reference_id: file:human/ELOB/ELOB-deep-research-falcon.md
      supporting_text: '**Elongin** was originally identified as a positive regulator of **RNA
        polymerase II** transcription elongation.'
- term:
    id: GO:0065003
    label: protein-containing complex assembly
  evidence_type: TAS
  original_reference_id: PMID:7660129
  review:
    summary: Accepted. ELOB helps assemble/stabilize Elongin BC-containing CRL complexes and
      transcription elongation complexes.
    action: ACCEPT
    additional_reference_ids:
    - file:human/ELOB/ELOB-deep-research-falcon.md
    supported_by:
    - reference_id: file:human/ELOB/ELOB-deep-research-falcon.md
      supporting_text: "for ELOB itself, the best-supported \u201Cprimary function\u201D in\
        \ mechanistic terms is **non-enzymatic**\u2014it serves as a **ubiquitin-like adaptor/regulatory\
        \ subunit** within Elongin BC"
    - reference_id: file:human/ELOB/ELOB-deep-research-falcon.md
      supporting_text: Elongin BC acts as an **adaptor** that couples substrate-recognition
        proteins bearing a **VHL box** or **SOCS box** to a Cullin scaffold
references:
- id: GO_REF:0000002
  title: Gene Ontology annotation through association of InterPro records with GO terms
  findings: []
- id: GO_REF:0000024
  title: Manual transfer of experimentally-verified manual GO annotation data to orthologs
    by curator judgment of sequence similarity
  findings: []
- id: GO_REF:0000033
  title: Annotation inferences using phylogenetic trees
  findings: []
- id: GO_REF:0000041
  title: Gene Ontology annotation based on UniPathway vocabulary mapping
  findings: []
- id: GO_REF:0000044
  title: Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary
    mapping, accompanied by conservative changes to GO terms applied by UniProt
  findings: []
- id: GO_REF:0000107
  title: Automatic transfer of experimentally verified manual GO annotation data to orthologs
    using Ensembl Compara
  findings: []
- id: GO_REF:0000117
  title: Electronic Gene Ontology annotations created by ARBA machine learning models
  findings: []
- id: GO_REF:0000120
  title: Combined Automated Annotation using Multiple IEA Methods
  findings: []
- id: PMID:10205047
  title: 'Structure of the VHL-ElonginC-ElonginB complex: implications for VHL tumor suppressor
    function.'
  findings: []
- id: PMID:12004076
  title: 'Structure of an HIF-1alpha -pVHL complex: hydroxyproline recognition in signaling.'
  findings: []
- id: PMID:12050673
  title: Structural basis for the recognition of hydroxyproline in HIF-1 alpha by pVHL.
  findings: []
- id: PMID:12149480
  title: Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble
    with Cul2 and Rbx1 to reconstitute a ubiquitin ligase.
  findings: []
- id: PMID:15590694
  title: Suppressors of cytokine signaling 4 and 5 regulate epidermal growth factor receptor
    signaling.
  findings: []
- id: PMID:15601820
  title: VHL-box and SOCS-box domains determine binding specificity for Cul2-Rbx1 and Cul5-Rbx2
    modules of ubiquitin ligases.
  findings: []
- id: PMID:16189514
  title: Towards a proteome-scale map of the human protein-protein interaction network.
  findings: []
- id: PMID:17636018
  title: ASB4 is a hydroxylation substrate of FIH and promotes vascular differentiation via
    an oxygen-dependent mechanism.
  findings: []
- id: PMID:18323857
  title: NEDD8 acts as a 'molecular switch' defining the functional selectivity of VHL.
  findings: []
- id: PMID:20211142
  title: An atlas of combinatorial transcriptional regulation in mouse and man.
  findings: []
- id: PMID:20871634
  title: Regulation of receptor for activated C kinase 1 protein by the von Hippel-Lindau
    tumor suppressor in IGF-I-induced renal carcinoma cell invasiveness.
  findings: []
- id: PMID:21119685
  title: Notch-induced Asb2 expression promotes protein ubiquitination by forming non-canonical
    E3 ligase complexes.
  findings: []
- id: PMID:21516116
  title: Next-generation sequencing to generate interactome datasets.
  findings: []
- id: PMID:21822215
  title: The tumour antigen PRAME is a subunit of a Cul2 ubiquitin ligase and associates with
    active NFY promoters.
  findings: []
- id: PMID:21980433
  title: The SOCS2 ubiquitin ligase complex regulates growth hormone receptor levels.
  findings: []
- id: PMID:22190034
  title: Global landscape of HIV-human protein complexes.
  findings: []
- id: PMID:22510880
  title: Nuclear receptor binding protein 1 regulates intestinal progenitor cell homeostasis
    and tumour formation.
  findings: []
- id: PMID:22649776
  title: The Role of Elongin BC-Containing Ubiquitin Ligases.
  findings: []
- id: PMID:23401859
  title: DCNL1 functions as a substrate sensor and activator of cullin 2-RING ligase.
  findings: []
- id: PMID:23837592
  title: 'Multimeric complexes among ankyrin-repeat and SOCS-box protein 9 (ASB9), ElonginBC,
    and Cullin 5: insights into the structure and assembly of ECS-type Cullin-RING E3 ubiquitin
    ligases.'
  findings: []
- id: PMID:25416956
  title: A proteome-scale map of the human interactome network.
  findings: []
- id: PMID:25505247
  title: 'Biophysical studies on interactions and assembly of full-size E3 ubiquitin ligase:
    suppressor of cytokine signaling 2 (SOCS2)-elongin BC-cullin 5-ring box protein 2 (RBX2).'
  findings: []
- id: PMID:26906416
  title: Characterization of the mammalian family of DCN-type NEDD8 E3 ligases.
  findings: []
- id: PMID:27517496
  title: USP9X destabilizes pVHL and promotes cell proliferation.
  findings: []
- id: PMID:28591624
  title: Crystal Structure of the Cul2-Rbx1-EloBC-VHL Ubiquitin Ligase Complex.
  findings: []
- id: PMID:29779948
  title: "The Eukaryotic Proteome Is Shaped by E3\_Ubiquitin Ligases Targeting C-Terminal\
    \ Degrons."
  findings: []
- id: PMID:30026228
  title: VHL substrate transcription factor ZHX2 as an oncogenic driver in clear cell renal
    cell carcinoma.
  findings: []
- id: PMID:31273098
  title: A glycine-specific N-degron pathway mediates the quality control of protein N-myristoylation.
  findings: []
- id: PMID:31387940
  title: BIK ubiquitination by the E3 ligase Cul5-ASB11 determines cell fate during cellular
    stress.
  findings: []
- id: PMID:32296183
  title: A reference map of the human binary protein interactome.
  findings: []
- id: PMID:32513959
  title: Structure and dynamics of the ASB9 CUL-RING E3 Ligase.
  findings: []
- id: PMID:33184237
  title: The ZSWIM8 ubiquitin ligase mediates target-directed microRNA degradation.
  findings: []
- id: PMID:33268465
  title: The Mechanism of NEDD8 Activation of CUL5 Ubiquitin E3 Ligases.
  findings: []
- id: PMID:33398168
  title: Molecular basis for arginine C-terminal degron recognition by Cul2(FEM1) E3 ligase.
  findings: []
- id: PMID:33909987
  title: Convergence of mammalian RQC and C-end rule proteolytic pathways via alanine tailing.
  findings: []
- id: PMID:33961781
  title: Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
  findings: []
- id: PMID:34743205
  title: CRL2-KLHDC3 E3 ubiquitin ligase complex suppresses ferroptosis through promoting
    p14(ARF) degradation.
  findings: []
- id: PMID:34857742
  title: Discovery of an exosite on the SOCS2-SH2 domain that enhances SH2 binding to phosphorylated
    ligands.
  findings: []
- id: PMID:35271311
  title: 'OpenCell: Endogenous tagging for the cartography of human cellular organization.'
  findings: []
- id: PMID:35512830
  title: Rab40c regulates focal adhesions and PP6 activity by controlling ANKRD28 ubiquitylation.
  findings: []
- id: PMID:35978186
  title: Post-translational control of beige fat biogenesis by PRDM16 stabilization.
  findings: []
- id: PMID:37816714
  title: Structure-based design of a phosphotyrosine-masked covalent ligand targeting the
    E3 ligase SOCS2.
  findings: []
- id: PMID:37844242
  title: Molecular basis for C-degron recognition by CRL2(APPBP2) ubiquitin ligase.
  findings: []
- id: PMID:38418882
  title: The CRL5-SPSB3 ubiquitin ligase targets nuclear cGAS for degradation.
  findings: []
- id: PMID:39039081
  title: Molecular insights into degron recognition by CRL5(ASB7) ubiquitin ligase.
  findings: []
- id: PMID:40205054
  title: Multimodal cell maps as a foundation for structural and functional genomics.
  findings: []
- id: PMID:40440427
  title: ASB7 is a negative regulator of H3K9me3 homeostasis.
  findings: []
- id: PMID:40963025
  title: Covariation MS uncovers a protein that controls cysteine catabolism.
  findings: []
- id: PMID:7660122
  title: Inhibition of transcription elongation by the VHL tumor suppressor protein.
  findings: []
- id: PMID:7660129
  title: 'Elongin (SIII): a multisubunit regulator of elongation by RNA polymerase II.'
  findings: []
- id: Reactome:R-HSA-112379
  title: Recruitment of elongation factors to form elongation complex
  findings: []
- id: Reactome:R-HSA-112385
  title: Addition of nucleotides leads to transcript elongation
  findings: []
- id: Reactome:R-HSA-112392
  title: Resumption of elongation after recovery from pausing
  findings: []
- id: Reactome:R-HSA-112395
  title: Abortive termination of elongation after arrest
  findings: []
- id: Reactome:R-HSA-112396
  title: Separation of elongating transcript from template
  findings: []
- id: Reactome:R-HSA-112435
  title: Formation of Elongin BC complex
  findings: []
- id: Reactome:R-HSA-112436
  title: Formation of Elongin complex
  findings: []
- id: Reactome:R-HSA-113411
  title: 2-4 nt.backtracking of Pol II complex on the template leading to elongation pausing
  findings: []
- id: Reactome:R-HSA-113412
  title: Pol II elongation complex moves on the template as transcript elongates
  findings: []
- id: Reactome:R-HSA-113413
  title: TFIIS-mediated recovery of elongation from arrest
  findings: []
- id: Reactome:R-HSA-113414
  title: 7-14 nt. Backtracking of Pol II complex on the template leading to elongation arrest
  findings: []
- id: Reactome:R-HSA-113429
  title: Elongating transcript encounters a lesion in the template
  findings: []
- id: Reactome:R-HSA-1234159
  title: Proteasome proteolyzes ub-HIF-alpha
  findings: []
- id: Reactome:R-HSA-1234163
  title: Cytosolic VBC complex ubiquitinylates hydroxyprolyl-HIF-alpha
  findings: []
- id: Reactome:R-HSA-1234169
  title: Nuclear VHL:EloB,C:CUL2:RBX1 binds hydroxyprolyl-HIF-alpha
  findings: []
- id: Reactome:R-HSA-1234172
  title: Nuclear VBC complex ubiquitinylates HIF-alpha
  findings: []
- id: Reactome:R-HSA-1234173
  title: Cytosolic PHD2,3 hydroxylates proline residues on HIF3A
  findings: []
- id: Reactome:R-HSA-1234175
  title: ub-hydroxyPro-HIF-alpha:VHL:EloB,C:CUL2:RBX1 translocates from the nucleus to the
    cytosol
  findings: []
- id: Reactome:R-HSA-1234177
  title: Cytosolic PHD2,3 hydroxylates proline residues on HIF1A
  findings: []
- id: Reactome:R-HSA-1234183
  title: Cytosolic VHL:EloB,C:CUL2:RBX1 binds hydroxyprolyl-HIF-alpha
  findings: []
- id: Reactome:R-HSA-180540
  title: Multi-ubiquitination of APOBEC3G
  findings: []
- id: Reactome:R-HSA-180555
  title: Association of APOBEC3G:Vif with the Cul5-SCF complex
  findings: []
- id: Reactome:R-HSA-180603
  title: Proteosome-mediated degradation of APOBEC3G
  findings: []
- id: Reactome:R-HSA-6797606
  title: CDK12 phosphorylates RNA Pol II CTD at DNA repair genes
  findings: []
- id: Reactome:R-HSA-6797616
  title: CCNK:CDK12 binds RNA Pol II at DNA repair genes
  findings: []
- id: Reactome:R-HSA-8952039
  title: NEDD8:AcM-UBE2F binds CRL5 E3 ubiquitin ligase complex
  findings: []
- id: Reactome:R-HSA-8952044
  title: AcM-UBE2F transfers NEDD8 to CRL5 E3 ubiquitin ligase complex
  findings: []
- id: Reactome:R-HSA-8952625
  title: NEDD8:AcM-UBE2M binds CRL2 E3 ubiquitin ligase complex
  findings: []
- id: Reactome:R-HSA-8952626
  title: AcM-UBE2M transfers NEDD8 to CRL2 E3 ubiquitin ligase complex
  findings: []
- id: Reactome:R-HSA-8955241
  title: CAND1 binds cytosolic CRL E3 ubiquitin ligases
  findings: []
- id: Reactome:R-HSA-8955289
  title: COMMDs displace CAND1 from cytosolic CRL E3 ubiquitin ligase complexes
  findings: []
- id: Reactome:R-HSA-8956040
  title: COP9 signalosome deneddylates cytosolic CRL E3 ubiquitin ligase complexes
  findings: []
- id: Reactome:R-HSA-8956099
  title: VHL:EloB,C:NEDD8-CUL2:RBX1 complex binds UBXN7
  findings: []
- id: Reactome:R-HSA-8956103
  title: VHL:EloB,C:NEDD8-CUL2:RBX1 complex binds hydroxyprolyl-HIF-alpha
  findings: []
- id: Reactome:R-HSA-8956106
  title: VHL:EloB,C:NEDD8-CUL2:RBX1 complex ubiquitinylates HIF-alpha
  findings: []
- id: Reactome:R-HSA-9705738
  title: SOCS1,3 ubiquitinates CSF3R in SOCS1,3:p-4Y-CSF3R:CSF3 dimer:LYN:p-Y-JAK1:p-JAK2:p-SYK:p-HCK:p-TYK2:CUL5:ELOB:ELOC:RNF7
  findings: []
- id: Reactome:R-HSA-9755303
  title: 26S proteasome degrades HIFalpha
  findings: []
- id: Reactome:R-HSA-9833107
  title: Association of NS1 (1C) with Cul5 and SCF
  findings: []
- id: Reactome:R-HSA-9833155
  title: Ubiquitination of STAT2
  findings: []
- id: Reactome:R-HSA-9954721
  title: NEDD8-K699-CUL2:ELOB:ELOC:RBX1:KLHDC10 K48 polyubiquitinates alanine-tailed nascent
    peptide
  findings: []
- id: Reactome:R-HSA-9954723
  title: NEDD8-K699-CUL2:ELOB:ELOC:RBX1:KLHDC10 binds alanine-tailed nascent peptide
  findings: []
- id: file:human/ELOB/ELOB-deep-research-falcon.md
  title: Falcon deep research report for human ELOB
  findings: []
core_functions:
- description: ELOB is a non-catalytic ubiquitin-ligase adaptor/regulatory subunit of Elongin
    BC that stabilizes transcription elongation and Cullin-RING ubiquitin ligase assemblies
    rather than acting as an enzyme or substrate receptor itself.
  supported_by:
  - reference_id: file:human/ELOB/ELOB-deep-research-falcon.md
    supporting_text: "for ELOB itself, the best-supported \u201Cprimary function\u201D in\
      \ mechanistic terms is **non-enzymatic**\u2014it serves as a **ubiquitin-like adaptor/regulatory\
      \ subunit** within Elongin BC"
  - reference_id: file:human/ELOB/ELOB-deep-research-falcon.md
    supporting_text: Elongin BC acts as an **adaptor** that couples substrate-recognition
      proteins bearing a **VHL box** or **SOCS box** to a Cullin scaffold
  - reference_id: file:human/ELOB/ELOB-deep-research-falcon.md
    supporting_text: '**Elongin** was originally identified as a positive regulator of **RNA
      polymerase II** transcription elongation.'
  molecular_function:
    id: GO:0031625
    label: ubiquitin protein ligase binding
  directly_involved_in:
  - id: GO:0006368
    label: transcription elongation by RNA polymerase II
  - id: GO:0016567
    label: protein ubiquitination
  - id: GO:0065003
    label: protein-containing complex assembly
  locations:
  - id: GO:0005654
    label: nucleoplasm
  - id: GO:0005829
    label: cytosol
  in_complex:
    id: GO:0070449
    label: elongin complex
proposed_new_terms: []
suggested_questions:
- question: Which ELOB/ELOC-containing CRL assemblies depend most strongly on ELOB-specific
    contacts rather than the shared ELOBC module?
  experts: []
- question: How separable are ELOB roles in classical Elongin transcription elongation from
    its CRL2/CRL5 ubiquitin ligase adaptor roles in human cells?
  experts: []
suggested_experiments:
- hypothesis: ELOB ubiquitin-like-domain surfaces differentially stabilize Elongin ABC versus
    CRL2/CRL5 assemblies.
  description: Rescue ELOB-depleted cells with interface mutants and compare RNA polymerase
    II elongation readouts, VHL-HIF turnover, and CRL5-SPSB3-dependent nuclear cGAS degradation.
- hypothesis: ELOB is required as a structural adaptor for substrate-receptor coupling rather
    than ubiquitin transfer catalysis.
  description: Reconstitute ELOBC-containing CRL2/CRL5 complexes with ELOB mutants and measure
    complex assembly, substrate binding, and ubiquitination kinetics.

ELOB

Gene Description

Existing Annotations Review

Core Functions

ELOB is a non-catalytic ubiquitin-ligase adaptor/regulatory subunit of Elongin BC that stabilizes transcription elongation and Cullin-RING ubiquitin ligase assemblies rather than acting as an enzyme or substrate receptor itself.

References

Suggested Questions for Experts

Suggested Experiments

Deep Research

Falcon

Research Report: Human ELOB (TCEB2; Elongin-B; UniProt Q15370)

0. Target verification (critical identity check)

1. Key concepts and definitions (current understanding)

1.1 Elongin-B as a regulatory subunit of the Elongin transcription elongation factor

1.2 Elongin BC as an adaptor module in Cullin-RING (CRL) ubiquitin ligases

1.3 Canonical pathway example: CRL2^VHL oxygen-sensing and HIF turnover

2. Molecular functions, complexes, localization, and pathways

2.1 Major complexes containing ELOB

2.2 Mechanistic notes and structural/biophysical constraints

3. Recent developments and latest research (prioritize 2023–2024)

3.1 2024: CRL5–SPSB3–ELOBC targets nuclear cGAS (high-confidence primary research)

3.2 2023: essentiality/fitness statistics for ELOB as a CRL2 subunit

3.3 2024: reviews emphasizing CRL modularity and ELOB/ELOC adaptors in targeted protein degradation

4. Current applications and real-world implementations

4.1 Targeted protein degradation (TPD): VHL/CRL2 systems as a widely used “E3 module”

4.2 Infection and viral immune evasion

4.3 Oxygen-sensing and cancer biology via VHL/HIF axis

5. Expert opinions and analysis (authoritative synthesis)

5.1 Two-function model: transcription elongation factor vs ubiquitin-ligase adaptor

5.2 “Modern” relevance: adaptor modules in immunity and precision protein control

6. Relevant statistics and data highlights

7. Consolidated evidence map (artifact)

8. Limitations and evidence gaps

Citations

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