ELOB encodes Elongin B, a small ubiquitin-like, non-catalytic regulatory/adaptor subunit of the Elongin BC module. Together with ELOC, it supports Elongin transcription elongation complexes and acts as a reusable adaptor module in Cul2/Cul5 Cullin-RING E3 ubiquitin ligases, including VHL, SOCS-box, viral, and recent CRL5-SPSB3 nuclear cGAS turnover contexts.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0070449 elongin complex | IBA GO_REF:0000033 | ACCEPT | Summary: Correct. ELOB is the ubiquitin-like Elongin B subunit of the Elongin BC regulatory module and Elongin ABC complex. Supporting Evidence: file:human/ELOB/ELOB-deep-research-falcon.md for ELOB itself, the best-supported “primary function” in mechanistic terms is **non-enzymatic**—it serves as a **ubiquitin-like adaptor/regulatory subunit** within Elongin BC |
| GO:0030891 VCB complex | IBA GO_REF:0000033 | ACCEPT | Summary: Correct. ELOB/ELOC form the Elongin BC module in the VHL-containing CRL2/VCB ubiquitin ligase complex. Supporting Evidence: file:human/ELOB/ELOB-deep-research-falcon.md In the **CRL2^VHL** ligase, the substrate receptor **pVHL** binds Elongin BC and engages **CUL2–RBX1** to ubiquitinate substrates |
| GO:0000151 ubiquitin ligase complex | IEA GO_REF:0000117 | MARK AS OVER ANNOTATED | Summary: Correct but broad. ELOB is a non-catalytic adaptor/regulatory subunit in more specific Cul2/Cul5 ubiquitin ligase complexes. Reason: More specific CRL2/CRL5 and VCB complex annotations capture the supported complex membership. |
| GO:0005634 nucleus | IEA GO_REF:0000044 | ACCEPT | Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts. |
| GO:0005737 cytoplasm | IEA GO_REF:0000117 | MARK AS OVER ANNOTATED | Summary: Correct but less specific than cytosol/nucleoplasm annotations for ELOB-containing complexes. Reason: Use cytosol and nucleoplasm where available rather than generic cytoplasm. |
| GO:0006368 transcription elongation by RNA polymerase II | IEA GO_REF:0000002 | ACCEPT | Summary: Accepted. ELOB is a regulatory Elongin subunit in the Elongin ABC transcription elongation factor that promotes RNA polymerase II elongation. Supporting Evidence: file:human/ELOB/ELOB-deep-research-falcon.md **Elongin** was originally identified as a positive regulator of **RNA polymerase II** transcription elongation. |
| GO:0030891 VCB complex | IEA GO_REF:0000002 | ACCEPT | Summary: Correct. ELOB/ELOC form the Elongin BC module in the VHL-containing CRL2/VCB ubiquitin ligase complex. Supporting Evidence: file:human/ELOB/ELOB-deep-research-falcon.md In the **CRL2^VHL** ligase, the substrate receptor **pVHL** binds Elongin BC and engages **CUL2–RBX1** to ubiquitinate substrates |
| GO:0070449 elongin complex | IEA GO_REF:0000120 | ACCEPT | Summary: Correct. ELOB is the ubiquitin-like Elongin B subunit of the Elongin BC regulatory module and Elongin ABC complex. Supporting Evidence: file:human/ELOB/ELOB-deep-research-falcon.md for ELOB itself, the best-supported “primary function” in mechanistic terms is **non-enzymatic**—it serves as a **ubiquitin-like adaptor/regulatory subunit** within Elongin BC |
| GO:0005515 protein binding | IPI PMID:10205047 Structure of the VHL-ElonginC-ElonginB complex: implications... | REMOVE | Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations. |
| GO:0005515 protein binding | IPI PMID:12004076 Structure of an HIF-1alpha -pVHL complex: hydroxyproline rec... | REMOVE | Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations. |
| GO:0005515 protein binding | IPI PMID:12050673 Structural basis for the recognition of hydroxyproline in HI... | REMOVE | Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations. |
| GO:0005515 protein binding | IPI PMID:12149480 Mammalian mediator subunit mMED8 is an Elongin BC-interactin... | REMOVE | Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations. |
| GO:0005515 protein binding | IPI PMID:15601820 VHL-box and SOCS-box domains determine binding specificity f... | REMOVE | Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations. |
| GO:0005515 protein binding | IPI PMID:16189514 Towards a proteome-scale map of the human protein-protein in... | REMOVE | Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations. |
| GO:0005515 protein binding | IPI PMID:18323857 NEDD8 acts as a 'molecular switch' defining the functional s... | REMOVE | Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations. |
| GO:0005515 protein binding | IPI PMID:20211142 An atlas of combinatorial transcriptional regulation in mous... | REMOVE | Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations. |
| GO:0005515 protein binding | IPI PMID:20871634 Regulation of receptor for activated C kinase 1 protein by t... | REMOVE | Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations. |
| GO:0005515 protein binding | IPI PMID:21516116 Next-generation sequencing to generate interactome datasets. | REMOVE | Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations. |
| GO:0005515 protein binding | IPI PMID:22190034 Global landscape of HIV-human protein complexes. | REMOVE | Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations. |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations. |
| GO:0005515 protein binding | IPI PMID:27517496 USP9X destabilizes pVHL and promotes cell proliferation. | REMOVE | Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations. |
| GO:0005515 protein binding | IPI PMID:30026228 VHL substrate transcription factor ZHX2 as an oncogenic driv... | REMOVE | Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations. |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | REMOVE | Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | REMOVE | Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations. |
| GO:0005515 protein binding | IPI PMID:40205054 Multimodal cell maps as a foundation for structural and func... | REMOVE | Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations. |
| GO:0016567 protein ubiquitination | IEA GO_REF:0000041 | ACCEPT | Summary: Supported as a broad process enabled by ELOB-containing CRL2/CRL5 ligases, though ELOB itself is a non-catalytic Elongin BC adaptor/regulatory subunit, not a substrate receptor or ubiquitin-transfer enzyme. Reason: Keep the process annotation because ELOB is a direct required module in CRL ubiquitination complexes. Do not add GO:1990756 for ELOB itself, because substrate recognition is supplied by SOCS-box, VHL-box, or related substrate receptor proteins rather than by ELOB. Supporting Evidence: file:human/ELOB/ELOB-deep-research-falcon.md Elongin BC acts as an **adaptor** that couples substrate-recognition proteins bearing a **VHL box** or **SOCS box** to a Cullin scaffold |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IEA GO_REF:0000107 | ACCEPT | Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:40963025 Covariation MS uncovers a protein that controls cysteine cat... | ACCEPT | Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors. |
| GO:0000122 negative regulation of transcription by RNA polymerase II | NAS PMID:28591624 Crystal Structure of the Cul2-Rbx1-EloBC-VHL Ubiquitin Ligas... | KEEP AS NON CORE | Summary: Context-specific transcriptional regulation annotation. ELOB core biology is Elongin/CRL adaptor function rather than a dedicated transcriptional repressor. Reason: Non-core transcription regulatory consequence of Elongin-containing complexes. |
| GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process | NAS PMID:31273098 A glycine-specific N-degron pathway mediates the quality con... | KEEP AS NON CORE | Summary: Supported as a broad consequence of ELOB-containing Cullin-RING ligase activity, but less specific than complex/adaptor annotations. Reason: ELOB is a non-catalytic adaptor/regulatory subunit within the ubiquitin ligase machinery. |
| GO:0140627 ubiquitin-dependent protein catabolic process via the C-end degron rule pathway | NAS PMID:15601820 VHL-box and SOCS-box domains determine binding specificity f... | KEEP AS NON CORE | Summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin ligase complexes, but not the general core molecular role of ELOB. Reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex function. |
| GO:0140627 ubiquitin-dependent protein catabolic process via the C-end degron rule pathway | NAS PMID:21822215 The tumour antigen PRAME is a subunit of a Cul2 ubiquitin li... | KEEP AS NON CORE | Summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin ligase complexes, but not the general core molecular role of ELOB. Reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex function. |
| GO:0140627 ubiquitin-dependent protein catabolic process via the C-end degron rule pathway | NAS PMID:22649776 The Role of Elongin BC-Containing Ubiquitin Ligases. | KEEP AS NON CORE | Summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin ligase complexes, but not the general core molecular role of ELOB. Reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex function. |
| GO:0140627 ubiquitin-dependent protein catabolic process via the C-end degron rule pathway | NAS PMID:29779948 The Eukaryotic Proteome Is Shaped by E3 Ubiquitin Ligases Ta... | KEEP AS NON CORE | Summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin ligase complexes, but not the general core molecular role of ELOB. Reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex function. |
| GO:0140627 ubiquitin-dependent protein catabolic process via the C-end degron rule pathway | NAS PMID:33398168 Molecular basis for arginine C-terminal degron recognition b... | KEEP AS NON CORE | Summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin ligase complexes, but not the general core molecular role of ELOB. Reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex function. |
| GO:0140627 ubiquitin-dependent protein catabolic process via the C-end degron rule pathway | NAS PMID:34743205 CRL2-KLHDC3 E3 ubiquitin ligase complex suppresses ferroptos... | KEEP AS NON CORE | Summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin ligase complexes, but not the general core molecular role of ELOB. Reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex function. |
| GO:1990116 ribosome-associated ubiquitin-dependent protein catabolic process | NAS PMID:33909987 Convergence of mammalian RQC and C-end rule proteolytic path... | KEEP AS NON CORE | Summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin ligase complexes, but not the general core molecular role of ELOB. Reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex function. |
| GO:2000104 negative regulation of DNA-templated DNA replication | NAS PMID:22649776 The Role of Elongin BC-Containing Ubiquitin Ligases. | KEEP AS NON CORE | Summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin ligase complexes, but not the general core molecular role of ELOB. Reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex function. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:39039081 Molecular insights into degron recognition by CRL5(ASB7) ubi... | ACCEPT | Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:40440427 ASB7 is a negative regulator of H3K9me3 homeostasis. | ACCEPT | Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors. |
| GO:0031462 Cul2-RING ubiquitin ligase complex | IDA PMID:35978186 Post-translational control of beige fat biogenesis by PRDM16... | ACCEPT | Summary: Correct. ELOB participates in Cul2-RING ubiquitin ligase assemblies such as CRL2-VHL through the Elongin BC adaptor module. Supporting Evidence: file:human/ELOB/ELOB-deep-research-falcon.md In the **CRL2^VHL** ligase, the substrate receptor **pVHL** binds Elongin BC and engages **CUL2–RBX1** to ubiquitinate substrates |
| GO:0031462 Cul2-RING ubiquitin ligase complex | IDA PMID:37844242 Molecular basis for C-degron recognition by CRL2(APPBP2) ubi... | ACCEPT | Summary: Correct. ELOB participates in Cul2-RING ubiquitin ligase assemblies such as CRL2-VHL through the Elongin BC adaptor module. Supporting Evidence: file:human/ELOB/ELOB-deep-research-falcon.md In the **CRL2^VHL** ligase, the substrate receptor **pVHL** binds Elongin BC and engages **CUL2–RBX1** to ubiquitinate substrates |
| GO:0140627 ubiquitin-dependent protein catabolic process via the C-end degron rule pathway | IDA PMID:37844242 Molecular basis for C-degron recognition by CRL2(APPBP2) ubi... | KEEP AS NON CORE | Summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin ligase complexes, but not the general core molecular role of ELOB. Reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex function. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:35512830 Rab40c regulates focal adhesions and PP6 activity by control... | ACCEPT | Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:37816714 Structure-based design of a phosphotyrosine-masked covalent ... | ACCEPT | Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:34857742 Discovery of an exosite on the SOCS2-SH2 domain that enhance... | ACCEPT | Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:21980433 The SOCS2 ubiquitin ligase complex regulates growth hormone ... | ACCEPT | Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:25505247 Biophysical studies on interactions and assembly of full-siz... | ACCEPT | Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:38418882 The CRL5-SPSB3 ubiquitin ligase targets nuclear cGAS for deg... | ACCEPT | Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:31387940 BIK ubiquitination by the E3 ligase Cul5-ASB11 determines ce... | ACCEPT | Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:32513959 Structure and dynamics of the ASB9 CUL-RING E3 Ligase. | ACCEPT | Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:23837592 Multimeric complexes among ankyrin-repeat and SOCS-box prote... | ACCEPT | Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:33268465 The Mechanism of NEDD8 Activation of CUL5 Ubiquitin E3 Ligas... | ACCEPT | Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors. |
| GO:0140958 target-directed miRNA degradation | IMP PMID:33184237 The ZSWIM8 ubiquitin ligase mediates target-directed microRN... | KEEP AS NON CORE | Summary: Supported as a substrate/pathway-specific output of ELOB-containing ubiquitin ligase complexes, but not the general core molecular role of ELOB. Reason: Specific downstream substrate pathway rather than ELOB's core adaptor/complex function. |
| GO:0005515 protein binding | IPI PMID:22510880 Nuclear receptor binding protein 1 regulates intestinal prog... | REMOVE | Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations. |
| GO:0001222 transcription corepressor binding | IPI PMID:7660122 Inhibition of transcription elongation by the VHL tumor supp... | KEEP AS NON CORE | Summary: Supported as a binding/regulatory-context annotation but secondary to ELOB's broader Elongin BC adaptor role. Reason: Non-core interaction-specific molecular function. |
| GO:0006367 transcription initiation at RNA polymerase II promoter | IDA PMID:7660122 Inhibition of transcription elongation by the VHL tumor supp... | REMOVE | Summary: Remove. The cited PMID:7660122 paper concerns VHL inhibition of Elongin-dependent RNA polymerase II transcription elongation, not transcription initiation. Reason: Evidence-term mismatch: PMID:7660122 supports transcription elongation biology, not GO:0006367 transcription initiation at RNA polymerase II promoter. |
| GO:0070449 elongin complex | IDA PMID:7660122 Inhibition of transcription elongation by the VHL tumor supp... | ACCEPT | Summary: Correct. ELOB is the ubiquitin-like Elongin B subunit of the Elongin BC regulatory module and Elongin ABC complex. Supporting Evidence: file:human/ELOB/ELOB-deep-research-falcon.md for ELOB itself, the best-supported “primary function” in mechanistic terms is **non-enzymatic**—it serves as a **ubiquitin-like adaptor/regulatory subunit** within Elongin BC |
| GO:0005515 protein binding | IPI PMID:21119685 Notch-induced Asb2 expression promotes protein ubiquitinatio... | REMOVE | Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations. |
| GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Supported as a broad consequence of ELOB-containing Cullin-RING ligase activity, but less specific than complex/adaptor annotations. Reason: ELOB is a non-catalytic adaptor/regulatory subunit within the ubiquitin ligase machinery. |
| GO:0005515 protein binding | IPI PMID:23401859 DCNL1 functions as a substrate sensor and activator of culli... | REMOVE | Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations. |
| GO:0005515 protein binding | IPI PMID:26906416 Characterization of the mammalian family of DCN-type NEDD8 E... | REMOVE | Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-1234159 | ACCEPT | Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-1234163 | ACCEPT | Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-1234173 | ACCEPT | Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-1234175 | ACCEPT | Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-1234177 | ACCEPT | Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-1234183 | ACCEPT | Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-180540 | ACCEPT | Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-180555 | ACCEPT | Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-180603 | ACCEPT | Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8952039 | ACCEPT | Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8952044 | ACCEPT | Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8952625 | ACCEPT | Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8952626 | ACCEPT | Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8955241 | ACCEPT | Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8955289 | ACCEPT | Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8956040 | ACCEPT | Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8956099 | ACCEPT | Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8956103 | ACCEPT | Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8956106 | ACCEPT | Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9705738 | ACCEPT | Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9755303 | ACCEPT | Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9833107 | ACCEPT | Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9833155 | ACCEPT | Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9954721 | ACCEPT | Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9954723 | ACCEPT | Summary: Supported broad localization for cytosolic CRL/adaptor contexts, including VHL/CRL and viral/SOCS-box ligase assemblies. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-112379 | ACCEPT | Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-112385 | ACCEPT | Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-112392 | ACCEPT | Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-112395 | ACCEPT | Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-112396 | ACCEPT | Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-112435 | ACCEPT | Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-112436 | ACCEPT | Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-113411 | ACCEPT | Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-113412 | ACCEPT | Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-113413 | ACCEPT | Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-113414 | ACCEPT | Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-113429 | ACCEPT | Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-1234169 | ACCEPT | Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-1234172 | ACCEPT | Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-1234175 | ACCEPT | Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6797606 | ACCEPT | Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6797616 | ACCEPT | Summary: Supported localization for nuclear Elongin/CRL functions, including RNA polymerase II elongation and nuclear cGAS turnover contexts. |
| GO:0031625 ubiquitin protein ligase binding | IDA PMID:17636018 ASB4 is a hydroxylation substrate of FIH and promotes vascul... | ACCEPT | Summary: Accepted. ELOB binds and stabilizes ubiquitin ligase assemblies as part of the Elongin BC adaptor module. Supporting Evidence: file:human/ELOB/ELOB-deep-research-falcon.md Elongin BC acts as an **adaptor** that couples substrate-recognition proteins bearing a **VHL box** or **SOCS box** to a Cullin scaffold |
| GO:0031462 Cul2-RING ubiquitin ligase complex | IDA PMID:17636018 ASB4 is a hydroxylation substrate of FIH and promotes vascul... | ACCEPT | Summary: Correct. ELOB participates in Cul2-RING ubiquitin ligase assemblies such as CRL2-VHL through the Elongin BC adaptor module. Supporting Evidence: file:human/ELOB/ELOB-deep-research-falcon.md In the **CRL2^VHL** ligase, the substrate receptor **pVHL** binds Elongin BC and engages **CUL2–RBX1** to ubiquitinate substrates |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:17636018 ASB4 is a hydroxylation substrate of FIH and promotes vascul... | ACCEPT | Summary: Correct. ELOB participates in Cul5-RING ubiquitin ligase assemblies through the Elongin BC adaptor module used by SOCS-box substrate receptors. |
| GO:0005515 protein binding | IPI PMID:15590694 Suppressors of cytokine signaling 4 and 5 regulate epidermal... | REMOVE | Summary: Protein binding is too generic for ELOB. The informative annotation is Elongin BC adaptor/regulatory subunit activity in Elongin and CRL complexes. Reason: Replace generic binding with specific complex/adaptor and ubiquitin-ligase-context annotations. |
| GO:0006368 transcription elongation by RNA polymerase II | TAS PMID:7660129 Elongin (SIII): a multisubunit regulator of elongation by RN... | ACCEPT | Summary: Accepted. ELOB is a regulatory Elongin subunit in the Elongin ABC transcription elongation factor that promotes RNA polymerase II elongation. Supporting Evidence: file:human/ELOB/ELOB-deep-research-falcon.md **Elongin** was originally identified as a positive regulator of **RNA polymerase II** transcription elongation. |
| GO:0065003 protein-containing complex assembly | TAS PMID:7660129 Elongin (SIII): a multisubunit regulator of elongation by RN... | ACCEPT | Summary: Accepted. ELOB helps assemble/stabilize Elongin BC-containing CRL complexes and transcription elongation complexes. Supporting Evidence: file:human/ELOB/ELOB-deep-research-falcon.md for ELOB itself, the best-supported “primary function” in mechanistic terms is **non-enzymatic**—it serves as a **ubiquitin-like adaptor/regulatory subunit** within Elongin BC file:human/ELOB/ELOB-deep-research-falcon.md Elongin BC acts as an **adaptor** that couples substrate-recognition proteins bearing a **VHL box** or **SOCS box** to a Cullin scaffold |
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Download this section (compressed HTML)Q: Which ELOB/ELOC-containing CRL assemblies depend most strongly on ELOB-specific contacts rather than the shared ELOBC module?
Q: How separable are ELOB roles in classical Elongin transcription elongation from its CRL2/CRL5 ubiquitin ligase adaptor roles in human cells?
Experiment: Rescue ELOB-depleted cells with interface mutants and compare RNA polymerase II elongation readouts, VHL-HIF turnover, and CRL5-SPSB3-dependent nuclear cGAS degradation.
Hypothesis: ELOB ubiquitin-like-domain surfaces differentially stabilize Elongin ABC versus CRL2/CRL5 assemblies.
Experiment: Reconstitute ELOBC-containing CRL2/CRL5 complexes with ELOB mutants and measure complex assembly, substrate binding, and ubiquitination kinetics.
Hypothesis: ELOB is required as a structural adaptor for substrate-receptor coupling rather than ubiquitin transfer catalysis.
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