ELOC encodes Elongin C, the SKP1-like adaptor subunit of the Elongin BC module. Together with ELOB, ELOC bridges BC-box, VHL-box, and SOCS-box substrate receptors to Cul2 or Cul5 Cullin-RING ubiquitin ligase scaffolds, while also participating in Elongin transcription elongation complexes.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0006511 ubiquitin-dependent protein catabolic process | IBA GO_REF:0000033 | ACCEPT | Summary: Accepted as a pathway-level consequence. ELOC-containing CRL2/CRL5 complexes target substrates for ubiquitination and downstream proteasomal degradation. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md A central and well-established role of ELOC is within the **VHL–ELOB/ELOC–CUL2–RBX1** E3 ubiquitin ligase |
| GO:0070449 elongin complex | IBA GO_REF:0000033 | ACCEPT | Summary: Correct. ELOC is Elongin C, the SKP1-like subunit of the Elongin BC module and Elongin complex. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md **ELOC (Elongin C)** forms a heterodimer with **ELOB (Elongin B)**, commonly referred to as the **Elongin BC** module. |
| GO:0030674 protein-macromolecule adaptor activity | IBA GO_REF:0000033 | ACCEPT | Summary: Correct and informative. ELOC acts as a protein-macromolecule adaptor within Elongin BC, bridging BC-box/VHL-box/SOCS-box substrate receptors to Cul2/Cul5 CRL scaffolds. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md The literature retrieved here consistently uses **ELOC** as the human gene encoding **Elongin C** (also called **TCEB1**) and describes it as the **Elongin B/C adaptor subunit** that binds **BC-box/VHL-box/SOCS-box** motifs and bridges substrate receptors to **CUL2** or **CUL5** in Cullin-RING ubiquitin ligases (CRLs), matching the UniProt-provided identity and SKP1-like adaptor role. |
| GO:0000151 ubiquitin ligase complex | IEA GO_REF:0000117 | MARK AS OVER ANNOTATED | Summary: Correct but broad. ELOC is better captured as an adaptor subunit in specific Cul2/Cul5 ubiquitin ligase complexes rather than by the generic ubiquitin ligase complex term alone. Reason: More specific Cul2-RING, Cul5-RING, and Elongin complex annotations capture the supported complex membership. |
| GO:0005634 nucleus | IEA GO_REF:0000044 | ACCEPT | Summary: Supported. ELOC has nuclear functions in Elongin/CRL assemblies and has experimentally observed nuclear/nucleolar localization. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md In human **HuH-7** cells, endogenous Elongin C shows **nuclear/nucleolar-like speckles** with **1:1 overlap with nucleolin**. |
| GO:0005737 cytoplasm | IEA GO_REF:0000117 | MARK AS OVER ANNOTATED | Summary: Correct but less precise than the available cytosol and nuclear/nucleoplasm localization annotations. Reason: Use cytosol and nucleus/nucleoplasm annotations where available rather than generic cytoplasm. |
| GO:0006511 ubiquitin-dependent protein catabolic process | IEA GO_REF:0000002 | ACCEPT | Summary: Accepted as a pathway-level consequence. ELOC-containing CRL2/CRL5 complexes target substrates for ubiquitination and downstream proteasomal degradation. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md A central and well-established role of ELOC is within the **VHL–ELOB/ELOC–CUL2–RBX1** E3 ubiquitin ligase |
| GO:0005515 protein binding | IPI PMID:10205047 Structure of the VHL-ElonginC-ElonginB complex: implications... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:12004076 Structure of an HIF-1alpha -pVHL complex: hydroxyproline rec... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:12050673 Structural basis for the recognition of hydroxyproline in HI... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:12149480 Mammalian mediator subunit mMED8 is an Elongin BC-interactin... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:15601820 VHL-box and SOCS-box domains determine binding specificity f... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:16189514 Towards a proteome-scale map of the human protein-protein in... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:16643902 The E3 ubiquitin ligase HOIL-1 induces the polyubiquitinatio... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:17183367 COMMD1 promotes the ubiquitination of NF-kappaB subunits thr... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:19159283 Array MAPPIT: high-throughput interactome analysis in mammal... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:19322197 Termination of NF-kappaB activity through a gammaherpesvirus... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:19327355 Requirement for microtubule integrity in the SOCS1-mediated ... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:20211142 An atlas of combinatorial transcriptional regulation in mous... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:21145461 Dynamics of cullin-RING ubiquitin ligase network revealed by... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:21516116 Next-generation sequencing to generate interactome datasets. | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:21822215 The tumour antigen PRAME is a subunit of a Cul2 ubiquitin li... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:21988832 Toward an understanding of the protein interaction network o... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:22190034 Global landscape of HIV-human protein complexes. | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:23460923 PRAME is a golgi-targeted protein that associates with the E... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:23563313 CCDC22 deficiency in humans blunts activation of proinflamma... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:28514442 Architecture of the human interactome defines protein commun... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:31515488 Extensive disruption of protein interactions by genetic vari... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:31980649 Extensive rewiring of the EGFR network in colorectal cancer ... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:32353859 A SARS-CoV-2 protein interaction map reveals targets for dru... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:33060197 Comparative host-coronavirus protein interaction networks re... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:33827988 ORF10-Cullin-2-ZYG11B complex is not required for SARS-CoV-2... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:36217030 A comprehensive SARS-CoV-2-human protein-protein interactome... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:40205054 Multimodal cell maps as a foundation for structural and func... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0016567 protein ubiquitination | IEA GO_REF:0000041 | ACCEPT | Summary: Accepted. ELOC is not the catalytic ubiquitin-transfer subunit, but it is required as an Elongin BC adaptor component of CRL2/CRL5 ligases that mediate substrate ubiquitination. Reason: Keep the process annotation because ELOC is a direct required module in CRL ubiquitination complexes. Do not add GO:1990756 for ELOC itself, because substrate recognition is supplied by SOCS-box, VHL-box, or related substrate receptor proteins rather than by ELOC. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md ELOC’s primary biochemical function in these contexts is **protein–protein interaction and complex assembly**, not ubiquitin transfer catalysis per se. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IEA GO_REF:0000107 | ACCEPT | Summary: Correct. ELOC is the adaptor subunit of Elongin BC in Cul5-RING ligase assemblies recruited by SOCS-box proteins. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md **CRL5** uses **CUL5** and **RBX2/SAG**, and commonly uses **SOCS-box** substrate receptors that recruit **ELOB/ELOC** as adaptors. |
| GO:0070449 elongin complex | IEA GO_REF:0000107 | ACCEPT | Summary: Correct. ELOC is Elongin C, the SKP1-like subunit of the Elongin BC module and Elongin complex. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md **ELOC (Elongin C)** forms a heterodimer with **ELOB (Elongin B)**, commonly referred to as the **Elongin BC** module. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:40963025 Covariation MS uncovers a protein that controls cysteine cat... | ACCEPT | Summary: Correct. ELOC is the adaptor subunit of Elongin BC in Cul5-RING ligase assemblies recruited by SOCS-box proteins. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md **CRL5** uses **CUL5** and **RBX2/SAG**, and commonly uses **SOCS-box** substrate receptors that recruit **ELOB/ELOC** as adaptors. |
| GO:0000122 negative regulation of transcription by RNA polymerase II | NAS PMID:28591624 Crystal Structure of the Cul2-Rbx1-EloBC-VHL Ubiquitin Ligas... | KEEP AS NON CORE | Summary: Kept as non-core. Negative transcriptional regulation can arise in specific Elongin-associated contexts, but it is not the central ELOC molecular function. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md The literature retrieved here consistently uses **ELOC** as the human gene encoding **Elongin C** (also called **TCEB1**) and describes it as the **Elongin B/C adaptor subunit** that binds **BC-box/VHL-box/SOCS-box** motifs and bridges substrate receptors to **CUL2** or **CUL5** in Cullin-RING ubiquitin ligases (CRLs), matching the UniProt-provided identity and SKP1-like adaptor role. |
| GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process | NAS PMID:31273098 A glycine-specific N-degron pathway mediates the quality con... | KEEP AS NON CORE | Summary: Supported as a non-core consequence of ELOC-containing CRL activity. ELOC is an adaptor/regulatory subunit rather than the proteasome or catalytic ubiquitin-transfer component. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md A central and well-established role of ELOC is within the **VHL–ELOB/ELOC–CUL2–RBX1** E3 ubiquitin ligase |
| GO:0140627 ubiquitin-dependent protein catabolic process via the C-end degron rule pathway | NAS PMID:15601820 VHL-box and SOCS-box domains determine binding specificity f... | KEEP AS NON CORE | Summary: Supported as a non-core specialized CRL2 pathway context. ELOC participates through the shared Elongin BC adaptor module, not as the substrate receptor itself. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md **CRL2** uses **CUL2** as scaffold and **RBX1** as the RING component, and uniquely uses **ELOB/ELOC** as adaptor proteins for substrate receptors like VHL. |
| GO:0140627 ubiquitin-dependent protein catabolic process via the C-end degron rule pathway | NAS PMID:21822215 The tumour antigen PRAME is a subunit of a Cul2 ubiquitin li... | KEEP AS NON CORE | Summary: Supported as a non-core specialized CRL2 pathway context. ELOC participates through the shared Elongin BC adaptor module, not as the substrate receptor itself. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md **CRL2** uses **CUL2** as scaffold and **RBX1** as the RING component, and uniquely uses **ELOB/ELOC** as adaptor proteins for substrate receptors like VHL. |
| GO:0140627 ubiquitin-dependent protein catabolic process via the C-end degron rule pathway | NAS PMID:22649776 The Role of Elongin BC-Containing Ubiquitin Ligases. | KEEP AS NON CORE | Summary: Supported as a non-core specialized CRL2 pathway context. ELOC participates through the shared Elongin BC adaptor module, not as the substrate receptor itself. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md **CRL2** uses **CUL2** as scaffold and **RBX1** as the RING component, and uniquely uses **ELOB/ELOC** as adaptor proteins for substrate receptors like VHL. |
| GO:0140627 ubiquitin-dependent protein catabolic process via the C-end degron rule pathway | NAS PMID:29779948 The Eukaryotic Proteome Is Shaped by E3 Ubiquitin Ligases Ta... | KEEP AS NON CORE | Summary: Supported as a non-core specialized CRL2 pathway context. ELOC participates through the shared Elongin BC adaptor module, not as the substrate receptor itself. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md **CRL2** uses **CUL2** as scaffold and **RBX1** as the RING component, and uniquely uses **ELOB/ELOC** as adaptor proteins for substrate receptors like VHL. |
| GO:0140627 ubiquitin-dependent protein catabolic process via the C-end degron rule pathway | NAS PMID:33398168 Molecular basis for arginine C-terminal degron recognition b... | KEEP AS NON CORE | Summary: Supported as a non-core specialized CRL2 pathway context. ELOC participates through the shared Elongin BC adaptor module, not as the substrate receptor itself. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md **CRL2** uses **CUL2** as scaffold and **RBX1** as the RING component, and uniquely uses **ELOB/ELOC** as adaptor proteins for substrate receptors like VHL. |
| GO:0140627 ubiquitin-dependent protein catabolic process via the C-end degron rule pathway | NAS PMID:34743205 CRL2-KLHDC3 E3 ubiquitin ligase complex suppresses ferroptos... | KEEP AS NON CORE | Summary: Supported as a non-core specialized CRL2 pathway context. ELOC participates through the shared Elongin BC adaptor module, not as the substrate receptor itself. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md **CRL2** uses **CUL2** as scaffold and **RBX1** as the RING component, and uniquely uses **ELOB/ELOC** as adaptor proteins for substrate receptors like VHL. |
| GO:1990116 ribosome-associated ubiquitin-dependent protein catabolic process | NAS PMID:33909987 Convergence of mammalian RQC and C-end rule proteolytic path... | KEEP AS NON CORE | Summary: Supported as a non-core specialized CRL pathway context. ELOC contributes through Elongin BC adaptor function in substrate-specific ligase assemblies. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md The literature retrieved here consistently uses **ELOC** as the human gene encoding **Elongin C** (also called **TCEB1**) and describes it as the **Elongin B/C adaptor subunit** that binds **BC-box/VHL-box/SOCS-box** motifs and bridges substrate receptors to **CUL2** or **CUL5** in Cullin-RING ubiquitin ligases (CRLs), matching the UniProt-provided identity and SKP1-like adaptor role. |
| GO:2000104 negative regulation of DNA-templated DNA replication | NAS PMID:22649776 The Role of Elongin BC-Containing Ubiquitin Ligases. | KEEP AS NON CORE | Summary: Kept as non-core. Negative regulation of DNA-templated DNA replication is likely a downstream substrate-specific consequence of ELOC-containing ligase activity rather than ELOC's primary molecular role. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md ELOC’s primary biochemical function in these contexts is **protein–protein interaction and complex assembly**, not ubiquitin transfer catalysis per se. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:39039081 Molecular insights into degron recognition by CRL5(ASB7) ubi... | ACCEPT | Summary: Correct. ELOC is the adaptor subunit of Elongin BC in Cul5-RING ligase assemblies recruited by SOCS-box proteins. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md **CRL5** uses **CUL5** and **RBX2/SAG**, and commonly uses **SOCS-box** substrate receptors that recruit **ELOB/ELOC** as adaptors. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:40440427 ASB7 is a negative regulator of H3K9me3 homeostasis. | ACCEPT | Summary: Correct. ELOC is the adaptor subunit of Elongin BC in Cul5-RING ligase assemblies recruited by SOCS-box proteins. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md **CRL5** uses **CUL5** and **RBX2/SAG**, and commonly uses **SOCS-box** substrate receptors that recruit **ELOB/ELOC** as adaptors. |
| GO:0031462 Cul2-RING ubiquitin ligase complex | IDA PMID:37844242 Molecular basis for C-degron recognition by CRL2(APPBP2) ubi... | ACCEPT | Summary: Correct. ELOC is the adaptor subunit of Elongin BC in Cul2-RING ligase assemblies such as CRL2-VHL. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md **CRL2** uses **CUL2** as scaffold and **RBX1** as the RING component, and uniquely uses **ELOB/ELOC** as adaptor proteins for substrate receptors like VHL. |
| GO:0140627 ubiquitin-dependent protein catabolic process via the C-end degron rule pathway | IDA PMID:37844242 Molecular basis for C-degron recognition by CRL2(APPBP2) ubi... | KEEP AS NON CORE | Summary: Supported as a non-core specialized CRL2 pathway context. ELOC participates through the shared Elongin BC adaptor module, not as the substrate receptor itself. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md **CRL2** uses **CUL2** as scaffold and **RBX1** as the RING component, and uniquely uses **ELOB/ELOC** as adaptor proteins for substrate receptors like VHL. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:35512830 Rab40c regulates focal adhesions and PP6 activity by control... | ACCEPT | Summary: Correct. ELOC is the adaptor subunit of Elongin BC in Cul5-RING ligase assemblies recruited by SOCS-box proteins. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md **CRL5** uses **CUL5** and **RBX2/SAG**, and commonly uses **SOCS-box** substrate receptors that recruit **ELOB/ELOC** as adaptors. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:37816714 Structure-based design of a phosphotyrosine-masked covalent ... | ACCEPT | Summary: Correct. ELOC is the adaptor subunit of Elongin BC in Cul5-RING ligase assemblies recruited by SOCS-box proteins. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md **CRL5** uses **CUL5** and **RBX2/SAG**, and commonly uses **SOCS-box** substrate receptors that recruit **ELOB/ELOC** as adaptors. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:34857742 Discovery of an exosite on the SOCS2-SH2 domain that enhance... | ACCEPT | Summary: Correct. ELOC is the adaptor subunit of Elongin BC in Cul5-RING ligase assemblies recruited by SOCS-box proteins. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md **CRL5** uses **CUL5** and **RBX2/SAG**, and commonly uses **SOCS-box** substrate receptors that recruit **ELOB/ELOC** as adaptors. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:21980433 The SOCS2 ubiquitin ligase complex regulates growth hormone ... | ACCEPT | Summary: Correct. ELOC is the adaptor subunit of Elongin BC in Cul5-RING ligase assemblies recruited by SOCS-box proteins. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md **CRL5** uses **CUL5** and **RBX2/SAG**, and commonly uses **SOCS-box** substrate receptors that recruit **ELOB/ELOC** as adaptors. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:25505247 Biophysical studies on interactions and assembly of full-siz... | ACCEPT | Summary: Correct. ELOC is the adaptor subunit of Elongin BC in Cul5-RING ligase assemblies recruited by SOCS-box proteins. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md **CRL5** uses **CUL5** and **RBX2/SAG**, and commonly uses **SOCS-box** substrate receptors that recruit **ELOB/ELOC** as adaptors. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:38418882 The CRL5-SPSB3 ubiquitin ligase targets nuclear cGAS for deg... | ACCEPT | Summary: Correct. ELOC is the adaptor subunit of Elongin BC in Cul5-RING ligase assemblies recruited by SOCS-box proteins. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md **CRL5** uses **CUL5** and **RBX2/SAG**, and commonly uses **SOCS-box** substrate receptors that recruit **ELOB/ELOC** as adaptors. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:31387940 BIK ubiquitination by the E3 ligase Cul5-ASB11 determines ce... | ACCEPT | Summary: Correct. ELOC is the adaptor subunit of Elongin BC in Cul5-RING ligase assemblies recruited by SOCS-box proteins. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md **CRL5** uses **CUL5** and **RBX2/SAG**, and commonly uses **SOCS-box** substrate receptors that recruit **ELOB/ELOC** as adaptors. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:32513959 Structure and dynamics of the ASB9 CUL-RING E3 Ligase. | ACCEPT | Summary: Correct. ELOC is the adaptor subunit of Elongin BC in Cul5-RING ligase assemblies recruited by SOCS-box proteins. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md **CRL5** uses **CUL5** and **RBX2/SAG**, and commonly uses **SOCS-box** substrate receptors that recruit **ELOB/ELOC** as adaptors. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:23837592 Multimeric complexes among ankyrin-repeat and SOCS-box prote... | ACCEPT | Summary: Correct. ELOC is the adaptor subunit of Elongin BC in Cul5-RING ligase assemblies recruited by SOCS-box proteins. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md **CRL5** uses **CUL5** and **RBX2/SAG**, and commonly uses **SOCS-box** substrate receptors that recruit **ELOB/ELOC** as adaptors. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:33268465 The Mechanism of NEDD8 Activation of CUL5 Ubiquitin E3 Ligas... | ACCEPT | Summary: Correct. ELOC is the adaptor subunit of Elongin BC in Cul5-RING ligase assemblies recruited by SOCS-box proteins. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md **CRL5** uses **CUL5** and **RBX2/SAG**, and commonly uses **SOCS-box** substrate receptors that recruit **ELOB/ELOC** as adaptors. |
| GO:0140958 target-directed miRNA degradation | IMP PMID:33184237 The ZSWIM8 ubiquitin ligase mediates target-directed microRN... | KEEP AS NON CORE | Summary: Kept as non-core. Target-directed miRNA degradation is a specialized pathway context for an ELOC-containing CRL assembly rather than the general function of ELOC itself. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md The literature retrieved here consistently uses **ELOC** as the human gene encoding **Elongin C** (also called **TCEB1**) and describes it as the **Elongin B/C adaptor subunit** that binds **BC-box/VHL-box/SOCS-box** motifs and bridges substrate receptors to **CUL2** or **CUL5** in Cullin-RING ubiquitin ligases (CRLs), matching the UniProt-provided identity and SKP1-like adaptor role. |
| GO:0005515 protein binding | IPI PMID:22510880 Nuclear receptor binding protein 1 regulates intestinal prog... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0001222 transcription corepressor binding | IPI PMID:7660122 Inhibition of transcription elongation by the VHL tumor supp... | KEEP AS NON CORE | Summary: Kept as non-core. ELOC can participate in transcriptional regulatory complexes, but its primary role is the Elongin BC adaptor/regulatory module. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md The literature retrieved here consistently uses **ELOC** as the human gene encoding **Elongin C** (also called **TCEB1**) and describes it as the **Elongin B/C adaptor subunit** that binds **BC-box/VHL-box/SOCS-box** motifs and bridges substrate receptors to **CUL2** or **CUL5** in Cullin-RING ubiquitin ligases (CRLs), matching the UniProt-provided identity and SKP1-like adaptor role. |
| GO:0006367 transcription initiation at RNA polymerase II promoter | IDA PMID:7660122 Inhibition of transcription elongation by the VHL tumor supp... | REMOVE | Summary: Remove. The cited PMID:7660122 paper concerns VHL inhibition of Elongin-dependent RNA polymerase II transcription elongation, not transcription initiation. Reason: Evidence-term mismatch: PMID:7660122 supports transcription elongation biology, not GO:0006367 transcription initiation at RNA polymerase II promoter. |
| GO:0070449 elongin complex | IDA PMID:7660122 Inhibition of transcription elongation by the VHL tumor supp... | ACCEPT | Summary: Correct. ELOC is Elongin C, the SKP1-like subunit of the Elongin BC module and Elongin complex. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md **ELOC (Elongin C)** forms a heterodimer with **ELOB (Elongin B)**, commonly referred to as the **Elongin BC** module. |
| GO:0016567 protein ubiquitination | IDA PMID:30166453 CRL4(AMBRA1) targets Elongin C for ubiquitination and degrad... | ACCEPT | Summary: Accepted. ELOC is not the catalytic ubiquitin-transfer subunit, but it is required as an Elongin BC adaptor component of CRL2/CRL5 ligases that mediate substrate ubiquitination. Reason: Keep the process annotation because ELOC is a direct required module in CRL ubiquitination complexes. Do not add GO:1990756 for ELOC itself, because substrate recognition is supplied by SOCS-box, VHL-box, or related substrate receptor proteins rather than by ELOC. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md ELOC’s primary biochemical function in these contexts is **protein–protein interaction and complex assembly**, not ubiquitin transfer catalysis per se. |
| GO:0031466 Cul5-RING ubiquitin ligase complex | IDA PMID:30166453 CRL4(AMBRA1) targets Elongin C for ubiquitination and degrad... | ACCEPT | Summary: Correct. ELOC is the adaptor subunit of Elongin BC in Cul5-RING ligase assemblies recruited by SOCS-box proteins. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md **CRL5** uses **CUL5** and **RBX2/SAG**, and commonly uses **SOCS-box** substrate receptors that recruit **ELOB/ELOC** as adaptors. |
| GO:0005515 protein binding | IPI PMID:21119685 Notch-induced Asb2 expression promotes protein ubiquitinatio... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0031462 Cul2-RING ubiquitin ligase complex | ISS GO_REF:0000024 | ACCEPT | Summary: Correct. ELOC is the adaptor subunit of Elongin BC in Cul2-RING ligase assemblies such as CRL2-VHL. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md **CRL2** uses **CUL2** as scaffold and **RBX1** as the RING component, and uniquely uses **ELOB/ELOC** as adaptor proteins for substrate receptors like VHL. |
| GO:0005515 protein binding | IPI PMID:21199876 Regulation of inducible nitric-oxide synthase by the SPRY do... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-1234159 | ACCEPT | Summary: Supported. ELOC-containing CRL2/CRL5 complexes and their substrate receptor assemblies operate in cytosolic as well as nuclear contexts. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-1234163 | ACCEPT | Summary: Supported. ELOC-containing CRL2/CRL5 complexes and their substrate receptor assemblies operate in cytosolic as well as nuclear contexts. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-1234173 | ACCEPT | Summary: Supported. ELOC-containing CRL2/CRL5 complexes and their substrate receptor assemblies operate in cytosolic as well as nuclear contexts. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-1234175 | ACCEPT | Summary: Supported. ELOC-containing CRL2/CRL5 complexes and their substrate receptor assemblies operate in cytosolic as well as nuclear contexts. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-1234177 | ACCEPT | Summary: Supported. ELOC-containing CRL2/CRL5 complexes and their substrate receptor assemblies operate in cytosolic as well as nuclear contexts. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-1234183 | ACCEPT | Summary: Supported. ELOC-containing CRL2/CRL5 complexes and their substrate receptor assemblies operate in cytosolic as well as nuclear contexts. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-180540 | ACCEPT | Summary: Supported. ELOC-containing CRL2/CRL5 complexes and their substrate receptor assemblies operate in cytosolic as well as nuclear contexts. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-180555 | ACCEPT | Summary: Supported. ELOC-containing CRL2/CRL5 complexes and their substrate receptor assemblies operate in cytosolic as well as nuclear contexts. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-180603 | ACCEPT | Summary: Supported. ELOC-containing CRL2/CRL5 complexes and their substrate receptor assemblies operate in cytosolic as well as nuclear contexts. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8952039 | ACCEPT | Summary: Supported. ELOC-containing CRL2/CRL5 complexes and their substrate receptor assemblies operate in cytosolic as well as nuclear contexts. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8952044 | ACCEPT | Summary: Supported. ELOC-containing CRL2/CRL5 complexes and their substrate receptor assemblies operate in cytosolic as well as nuclear contexts. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8952625 | ACCEPT | Summary: Supported. ELOC-containing CRL2/CRL5 complexes and their substrate receptor assemblies operate in cytosolic as well as nuclear contexts. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8952626 | ACCEPT | Summary: Supported. ELOC-containing CRL2/CRL5 complexes and their substrate receptor assemblies operate in cytosolic as well as nuclear contexts. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8955241 | ACCEPT | Summary: Supported. ELOC-containing CRL2/CRL5 complexes and their substrate receptor assemblies operate in cytosolic as well as nuclear contexts. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8955289 | ACCEPT | Summary: Supported. ELOC-containing CRL2/CRL5 complexes and their substrate receptor assemblies operate in cytosolic as well as nuclear contexts. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8956040 | ACCEPT | Summary: Supported. ELOC-containing CRL2/CRL5 complexes and their substrate receptor assemblies operate in cytosolic as well as nuclear contexts. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8956099 | ACCEPT | Summary: Supported. ELOC-containing CRL2/CRL5 complexes and their substrate receptor assemblies operate in cytosolic as well as nuclear contexts. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8956103 | ACCEPT | Summary: Supported. ELOC-containing CRL2/CRL5 complexes and their substrate receptor assemblies operate in cytosolic as well as nuclear contexts. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8956106 | ACCEPT | Summary: Supported. ELOC-containing CRL2/CRL5 complexes and their substrate receptor assemblies operate in cytosolic as well as nuclear contexts. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9705738 | ACCEPT | Summary: Supported. ELOC-containing CRL2/CRL5 complexes and their substrate receptor assemblies operate in cytosolic as well as nuclear contexts. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9755303 | ACCEPT | Summary: Supported. ELOC-containing CRL2/CRL5 complexes and their substrate receptor assemblies operate in cytosolic as well as nuclear contexts. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9833107 | ACCEPT | Summary: Supported. ELOC-containing CRL2/CRL5 complexes and their substrate receptor assemblies operate in cytosolic as well as nuclear contexts. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9833155 | ACCEPT | Summary: Supported. ELOC-containing CRL2/CRL5 complexes and their substrate receptor assemblies operate in cytosolic as well as nuclear contexts. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9954721 | ACCEPT | Summary: Supported. ELOC-containing CRL2/CRL5 complexes and their substrate receptor assemblies operate in cytosolic as well as nuclear contexts. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9954723 | ACCEPT | Summary: Supported. ELOC-containing CRL2/CRL5 complexes and their substrate receptor assemblies operate in cytosolic as well as nuclear contexts. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-112379 | ACCEPT | Summary: Supported. ELOC has a prominent nuclear/nucleoplasmic pool consistent with Elongin transcriptional and nuclear CRL functions. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md In human **HuH-7** cells, endogenous Elongin C shows **nuclear/nucleolar-like speckles** with **1:1 overlap with nucleolin**. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-112385 | ACCEPT | Summary: Supported. ELOC has a prominent nuclear/nucleoplasmic pool consistent with Elongin transcriptional and nuclear CRL functions. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md In human **HuH-7** cells, endogenous Elongin C shows **nuclear/nucleolar-like speckles** with **1:1 overlap with nucleolin**. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-112392 | ACCEPT | Summary: Supported. ELOC has a prominent nuclear/nucleoplasmic pool consistent with Elongin transcriptional and nuclear CRL functions. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md In human **HuH-7** cells, endogenous Elongin C shows **nuclear/nucleolar-like speckles** with **1:1 overlap with nucleolin**. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-112395 | ACCEPT | Summary: Supported. ELOC has a prominent nuclear/nucleoplasmic pool consistent with Elongin transcriptional and nuclear CRL functions. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md In human **HuH-7** cells, endogenous Elongin C shows **nuclear/nucleolar-like speckles** with **1:1 overlap with nucleolin**. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-112396 | ACCEPT | Summary: Supported. ELOC has a prominent nuclear/nucleoplasmic pool consistent with Elongin transcriptional and nuclear CRL functions. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md In human **HuH-7** cells, endogenous Elongin C shows **nuclear/nucleolar-like speckles** with **1:1 overlap with nucleolin**. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-112435 | ACCEPT | Summary: Supported. ELOC has a prominent nuclear/nucleoplasmic pool consistent with Elongin transcriptional and nuclear CRL functions. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md In human **HuH-7** cells, endogenous Elongin C shows **nuclear/nucleolar-like speckles** with **1:1 overlap with nucleolin**. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-112436 | ACCEPT | Summary: Supported. ELOC has a prominent nuclear/nucleoplasmic pool consistent with Elongin transcriptional and nuclear CRL functions. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md In human **HuH-7** cells, endogenous Elongin C shows **nuclear/nucleolar-like speckles** with **1:1 overlap with nucleolin**. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-113411 | ACCEPT | Summary: Supported. ELOC has a prominent nuclear/nucleoplasmic pool consistent with Elongin transcriptional and nuclear CRL functions. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md In human **HuH-7** cells, endogenous Elongin C shows **nuclear/nucleolar-like speckles** with **1:1 overlap with nucleolin**. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-113412 | ACCEPT | Summary: Supported. ELOC has a prominent nuclear/nucleoplasmic pool consistent with Elongin transcriptional and nuclear CRL functions. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md In human **HuH-7** cells, endogenous Elongin C shows **nuclear/nucleolar-like speckles** with **1:1 overlap with nucleolin**. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-113413 | ACCEPT | Summary: Supported. ELOC has a prominent nuclear/nucleoplasmic pool consistent with Elongin transcriptional and nuclear CRL functions. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md In human **HuH-7** cells, endogenous Elongin C shows **nuclear/nucleolar-like speckles** with **1:1 overlap with nucleolin**. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-113414 | ACCEPT | Summary: Supported. ELOC has a prominent nuclear/nucleoplasmic pool consistent with Elongin transcriptional and nuclear CRL functions. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md In human **HuH-7** cells, endogenous Elongin C shows **nuclear/nucleolar-like speckles** with **1:1 overlap with nucleolin**. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-113429 | ACCEPT | Summary: Supported. ELOC has a prominent nuclear/nucleoplasmic pool consistent with Elongin transcriptional and nuclear CRL functions. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md In human **HuH-7** cells, endogenous Elongin C shows **nuclear/nucleolar-like speckles** with **1:1 overlap with nucleolin**. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-1234169 | ACCEPT | Summary: Supported. ELOC has a prominent nuclear/nucleoplasmic pool consistent with Elongin transcriptional and nuclear CRL functions. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md In human **HuH-7** cells, endogenous Elongin C shows **nuclear/nucleolar-like speckles** with **1:1 overlap with nucleolin**. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-1234172 | ACCEPT | Summary: Supported. ELOC has a prominent nuclear/nucleoplasmic pool consistent with Elongin transcriptional and nuclear CRL functions. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md In human **HuH-7** cells, endogenous Elongin C shows **nuclear/nucleolar-like speckles** with **1:1 overlap with nucleolin**. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-1234175 | ACCEPT | Summary: Supported. ELOC has a prominent nuclear/nucleoplasmic pool consistent with Elongin transcriptional and nuclear CRL functions. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md In human **HuH-7** cells, endogenous Elongin C shows **nuclear/nucleolar-like speckles** with **1:1 overlap with nucleolin**. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6797606 | ACCEPT | Summary: Supported. ELOC has a prominent nuclear/nucleoplasmic pool consistent with Elongin transcriptional and nuclear CRL functions. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md In human **HuH-7** cells, endogenous Elongin C shows **nuclear/nucleolar-like speckles** with **1:1 overlap with nucleolin**. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6797616 | ACCEPT | Summary: Supported. ELOC has a prominent nuclear/nucleoplasmic pool consistent with Elongin transcriptional and nuclear CRL functions. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md In human **HuH-7** cells, endogenous Elongin C shows **nuclear/nucleolar-like speckles** with **1:1 overlap with nucleolin**. |
| GO:0005515 protein binding | IPI PMID:15590694 Suppressors of cytokine signaling 4 and 5 regulate epidermal... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0005515 protein binding | IPI PMID:10851083 Tracheal development and the von Hippel-Lindau tumor suppres... | REMOVE | Summary: Protein binding is too generic for ELOC. The informative annotation is Elongin BC adaptor activity and specific Cul2/Cul5 ligase complex membership. Reason: Replace generic binding with specific adaptor and CRL complex annotations. |
| GO:0006357 regulation of transcription by RNA polymerase II | TAS PMID:7660129 Elongin (SIII): a multisubunit regulator of elongation by RN... | KEEP AS NON CORE | Summary: Kept as non-core. Regulation of RNA polymerase II transcription is a supported broader context, but ELOC is best captured by Elongin BC adaptor and CRL complex roles. Supporting Evidence: file:human/ELOC/ELOC-deep-research-falcon.md The literature retrieved here consistently uses **ELOC** as the human gene encoding **Elongin C** (also called **TCEB1**) and describes it as the **Elongin B/C adaptor subunit** that binds **BC-box/VHL-box/SOCS-box** motifs and bridges substrate receptors to **CUL2** or **CUL5** in Cullin-RING ubiquitin ligases (CRLs), matching the UniProt-provided identity and SKP1-like adaptor role. |
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Download this section (compressed HTML)Q: Which ELOC-containing CRL assemblies are most sensitive to ELOC interface variants such as Y79C, and how do these differ from VHL loss?
Q: How separable are ELOC roles in classical Elongin transcription elongation from its dominant CRL2/CRL5 adaptor functions across human cell types?
Experiment: Rescue ELOC-depleted cells with interface mutants and compare VHL-HIF turnover, SOCS-box CRL5 substrate degradation, and RNA polymerase II elongation readouts.
Hypothesis: ELOC interface residues differentially support CRL2-VHL, CRL5-SOCS, and Elongin transcription elongation assemblies.
Experiment: Reconstitute ELOBC-containing CRL2 and CRL5 complexes with wild-type and mutant ELOC and measure complex formation, substrate receptor recruitment, and ubiquitination kinetics.
Hypothesis: ELOC promotes ubiquitin-dependent substrate degradation through adaptor-mediated complex assembly rather than catalytic ubiquitin transfer.
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