ERCC2 encodes XPD, an iron–sulfur DNA helicase and structural subunit of the general transcription factor TFIIH. The canonical 760-residue protein uses ATP to unwind DNA in the 5′–3′ direction during nucleotide-excision repair. Its repair activity is coordinated with XPB, XPA and XPG, while the nuclease reactions are performed by other repair proteins. In RNA-polymerase-II transcription initiation, XPD helps organize TFIIH and connect its core to the CDK-activating kinase module through protein contacts; XPD itself is not the associated protein kinase. XPD is predominantly nuclear, with a cytoplasmic pool that interacts with the CIA iron–sulfur maturation machinery as a client. A reported 405-residue splice isoform lacks much of the canonical C-terminal region, and its biochemical activity and cellular roles remain unestablished.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0000439 transcription factor TFIIH core complex | IDA PMID:8692841 Isolation and characterization of two human transcription fa... | ACCEPT | Summary: XPD is a constituent of the human TFIIH core. Reason: PMID:8692841 Methods and Figures 2–5 characterize human TFIIH-related fractions. Its historical TFIIH* preparation lacks XPD and is not identical to the current seven-subunit GO core definition. Independent recombinant human TFIIH and structural evidence in PMID:31253769 establish XPD within the seven-subunit core; retain the current complex annotation with this nomenclature distinction. |
| GO:0000439 transcription factor TFIIH core complex | IDA PMID:8692842 Human cyclin-dependent kinase-activating kinase exists in th... | ACCEPT | Summary: XPD is present in purified human TFIIH and belongs to its seven-subunit core. Reason: PMID:8692842 Methods and Figure 1 identify ERCC2 in human HeLa nuclear TFIIH. The current core composition is independently explicit in PMID:31253769 Methods and Figure 2. CAK kinase activity must not be assigned to the XPD polypeptide. |
| GO:0000439 transcription factor TFIIH core complex | IEA GO_REF:0000107 | ACCEPT | Summary: ERCC2 is the XPD subunit of the TFIIH core. Reason: The rat donor A0A8I6AH26/ENSRNOP00000092445 carries an IDA complex annotation from PMID:8855220; only that original abstract and scanned-page landing record were accessible here. Independently read human experiments establish the assertion: recombinant human seven-subunit core TFIIH contains XPD (PMID:31253769, Results/Figure 1 and Methods constructs, expression and purification), and human XPD-GFP co-immunoprecipitates XPB while rescuing XP-D fibroblast UV sensitivity (PMID:16669699, Figure 1). No functional equivalence is inferred for the 405-aa natural product. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:A0A8I6AH26 SUPPORTS TRANSFER Official rat record and current GOA connect TFIIH-core IDA to PMID:8855220; independently read human TFIIH experiments resolve the target complex assignment. ensembl:ENSRNOP00000092445 SUPPORTS TRANSFER The official rat UniProt cross-reference verifies this imported ortholog identifier; no accession correction is needed. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0001666 response to hypoxia | IEA GO_REF:0000107 | UNDECIDED | Summary: The rat hypoxia-expression result is positive, but its transfer to human ERCC2 remains insufficiently adjudicated. Reason: Current rat donor GOA traces this annotation to IEP PMID:9763211. Its verified abstract reports ERCC2 mRNA induction in rat brain after perinatal asphyxia; the full original Methods/Results could not be recovered. GO:0001666 can include gene-expression responses, so expression is not categorically incompatible with the term. The accessible evidence does not settle the conserved human response or the experimental scope of this source; retain uncertainty rather than infer absence of a response. Propagation Review Root cause: UNRESOLVED Sources checked: UniProtKB:A0A8I6AH26 UNRESOLVED Rat donor IEP PMID:9763211 is positively supported by its abstract, but the original and target response scope remain unresolved. ensembl:ENSRNOP00000092445 SUPPORTS TRANSFER Official ortholog identity is verified; the uncertainty concerns response evidence rather than orthology. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0003676 nucleic acid binding | IEA GO_REF:0000002 | MODIFY | Summary: The broad nucleic-acid-binding domain annotation can be specified as DNA binding. Reason: IPR006555 is a helicase C-terminal domain with a broad nucleic-acid-binding mapping. Actual human core-TFIIH structures place XPD on single-stranded DNA in a repair-bubble scaffold (PMID:31253769, Results and Figures 2–4); human XPD-dependent DNA unwinding is independently measured (PMID:19934020, Figure 5 and Methods). These sources support DNA binding without inferring an RNA-substrate activity from the domain family. Proposed replacements: DNA binding Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0003677 DNA binding | IEA GO_REF:0000002 | ACCEPT | Summary: Human XPD directly engages DNA. Reason: Target membership in IPR001945 and IPR010614 is verified against the official UniProt record. Human TFIIH structures and DNA-unwinding experiments directly support DNA engagement (PMID:31253769, Results/Figures 2–4; PMID:19934020, Figure 5). The structural DNA scaffold is lesion-free, so this evidence is not presented as a direct assay of human damaged-DNA selectivity. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0003678 DNA helicase activity | IBA GO_REF:0000033 | MODIFY | Summary: Specify the established XPD helicase directionality. Reason: The PAINT IBD is placed at PTN000158192 (LUCA) in PTHR11472 and validly encompasses the target. Human recombinant XPD/TFIIH unwinds DNA in the 5′–3′ direction: PMID:19934020 Figure 5 and helicase Methods separate helicase activity from ATPase, while PMID:31253769 Results/Figure 1 and helicase Methods show XPD-dependent unwinding abolished by XPD K48R. The more specific GO:0043139 represents the target activity; this refinement does not reject the ancestral generic helicase assertion or imply RNA helicase activity. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000158192 SUPPORTS TRANSFER The saved IBD record asserts GO:0003678 at the ancestral node; direct human experiments refine the target activity to its established 5′–3′ direction. The target leaf PTN002479324 descends from this exact node in the official PTHR11472 tree; its aligned sequence matches canonical human P18074 (760 aa), and no NOT record lies on its saved IBD target path. Proposed replacements: 5'-3' DNA helicase activity Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0003678 DNA helicase activity | IEA GO_REF:0000002 | MODIFY | Summary: Specify the established XPD helicase directionality. Reason: The exact imported InterPro entries occur in the target UniProt cross-references and their official GO mappings support a helicase domain/family. Human recombinant XPD/TFIIH unwinds DNA in the 5′–3′ direction: PMID:19934020 Figure 5 and helicase Methods separate helicase activity from ATPase, while PMID:31253769 Results/Figure 1 and helicase Methods show XPD-dependent unwinding abolished by XPD K48R. The more specific GO:0043139 represents the target activity; this refinement does not reject the ancestral generic helicase assertion or imply RNA helicase activity. Proposed replacements: 5'-3' DNA helicase activity Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0003684 damaged DNA binding | IBA GO_REF:0000033 | ACCEPT | Summary: The damaged-DNA-binding IBA has a verified ancestral node and an experimental yeast descendant source. Reason: The exact GO:0003684 IBD at PANTHER:PTN000158239 encompasses human XPD and yeast RAD3. Current yeast GOA links RAD3/P06839 to IDA PMID:8132553. Its actual original Methods and Results/Figures 1, 3 and 4 measure purified yeast RAD3 binding to UV-damaged versus undamaged DNA, with ATP and DNA-topology controls. This experimentally grounded node supports the conserved inference. Human TFIIH repair and DNA-contact evidence is compatible (PMID:19934020, Figure 5; PMID:31253769, Figures 2–4), but neither is relabeled as a human damage-preference binding assay. One experimental donor is not weak phylogenetic evidence, and no target-specific loss is established. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000158239 SUPPORTS TRANSFER The IBD GO:0003684 record identifies SGD:S000000973; its yeast RAD3 IDA PMID:8132553 original directly measures damaged-DNA preference. The original is yeast, not a human binding assay. The target leaf PTN002479324 descends from this exact node in the official PTHR11472 tree; its aligned sequence matches canonical human P18074 (760 aa), and no NOT record lies on its saved IBD target path. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0004386 helicase activity | IEA GO_REF:0000002 | MODIFY | Summary: Specify the established XPD helicase directionality. Reason: The exact imported InterPro entries occur in the target UniProt cross-references and their official GO mappings support a helicase domain/family. Human recombinant XPD/TFIIH unwinds DNA in the 5′–3′ direction: PMID:19934020 Figure 5 and helicase Methods separate helicase activity from ATPase, while PMID:31253769 Results/Figure 1 and helicase Methods show XPD-dependent unwinding abolished by XPD K48R. The more specific GO:0043139 represents the target activity; this refinement does not reject the ancestral generic helicase assertion or imply RNA helicase activity. Proposed replacements: 5'-3' DNA helicase activity Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005515 protein binding | IPI PMID:10801852 Distinct regions of MAT1 regulate cdk7 kinase and TFIIH tran... | MODIFY | Summary: XPD–MAT1 association supports the scaffold connection between CAK and core TFIIH. Reason: The cited original reports MAT1–XPD interaction, but the complete publisher article was not recovered; for PMID:11445587 only indexed original Methods/initial Results were recovered. Independent human CAK–ERCC2 purification and transcription reconstitution in PMID:8692842 Figures 3–6, together with the XPD–MAT1 interface and module-dependent architecture in PMID:31253769 Figure 4, support the protein-complex scaffold role. This is complex organization, not XPD kinase activity. Current GO:0030674 explicitly distinguishes integral protein-complex scaffolds from generic adaptors. XPD is an integral TFIIH subunit whose human assembly/reconstitution and interface evidence supports holding the p44-associated core and CAK module together. Refine this structural role to GO:0140378 protein complex scaffold activity. This does not claim transient recruitment of a particular kinase substrate, kinase catalysis by XPD, or equivalent function of the 405-residue natural product. Proposed replacements: protein complex scaffold activity Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005515 protein binding | IPI PMID:11445587 A yeast four-hybrid system identifies Cdk-activating kinase ... | MODIFY | Summary: XPD–MAT1 association supports the scaffold connection between CAK and core TFIIH. Reason: The cited original reports MAT1–XPD interaction, but the complete publisher article was not recovered; for PMID:11445587 only indexed original Methods/initial Results were recovered. Independent human CAK–ERCC2 purification and transcription reconstitution in PMID:8692842 Figures 3–6, together with the XPD–MAT1 interface and module-dependent architecture in PMID:31253769 Figure 4, support the protein-complex scaffold role. This is complex organization, not XPD kinase activity. Current GO:0030674 explicitly distinguishes integral protein-complex scaffolds from generic adaptors. XPD is an integral TFIIH subunit whose human assembly/reconstitution and interface evidence supports holding the p44-associated core and CAK module together. Refine this structural role to GO:0140378 protein complex scaffold activity. This does not claim transient recruitment of a particular kinase substrate, kinase catalysis by XPD, or equivalent function of the 405-residue natural product. Proposed replacements: protein complex scaffold activity Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005515 protein binding | IPI PMID:11445587 A yeast four-hybrid system identifies Cdk-activating kinase ... | MODIFY | Summary: The XPD–p44 interface helps connect the CAK-associated XPD subunit to core TFIIH. Reason: The full PMID:11445587 article remains incompletely accessible. Independent human XPD/p44 coexpression and high-salt interaction assays in PMID:19934020 Figure 4D, combined with the TFIIH integrity and transcription assays in Figures 4–6, establish the functional anchoring role. Replace generic binding with the experimentally supported protein-complex scaffold activity, without implying that p44 is a kinase. Current GO:0030674 explicitly distinguishes integral protein-complex scaffolds from generic adaptors. XPD is an integral TFIIH subunit whose human assembly/reconstitution and interface evidence supports holding the p44-associated core and CAK module together. Refine this structural role to GO:0140378 protein complex scaffold activity. This does not claim transient recruitment of a particular kinase substrate, kinase catalysis by XPD, or equivalent function of the 405-residue natural product. Proposed replacements: protein complex scaffold activity Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005515 protein binding | IPI PMID:16669699 Dynamic interaction of TTDA with TFIIH is stabilized by nucl... | KEEP AS NON CORE | Summary: Full-length human XPD-GFP associates with XPB in TFIIH. Reason: PMID:16669699 Methods explicitly identify full-length human XPD-GFP in XP-D fibroblasts, and Figure 1D recovers XPB with the fusion protein. This is supported complex association; the assay does not isolate a separate molecular activity for the XPD–XPB contact. Retain generic binding as non-core. Supporting Evidence: |
| GO:0005515 protein binding | IPI PMID:19934020 Both XPD alleles contribute to the phenotype of compound het... | KEEP AS NON CORE | Summary: Human XPD and XPB coassociate in TFIIH. Reason: PMID:19934020 Figure 3C recovers expressed XPD species in XPB-directed chromatin immunoprecipitates, with other TFIIH subunits. The assay supports complex association rather than purified binary binding or a separately established activity for this pair. Retain the supported generic interaction as non-core. Supporting Evidence: |
| GO:0005515 protein binding | IPI PMID:19934020 Both XPD alleles contribute to the phenotype of compound het... | MODIFY | Summary: The human XPD–p44 interaction contributes to TFIIH assembly and the CAK-to-core connection. Reason: PMID:19934020 Figure 4D and the interaction Methods test XPD/p44 association with WT and patient-derived XPD variants; Figures 4–6 connect weakened association and complex integrity to repair/transcription defects. Together with native human CAK–ERCC2 reconstitution in PMID:8692842, this supports the protein-complex scaffold role. These are full-length or engineered disease-variant preparations, not evidence for equivalent activity of the natural 405-aa product. Current GO:0030674 explicitly distinguishes integral protein-complex scaffolds from generic adaptors. XPD is an integral TFIIH subunit whose human assembly/reconstitution and interface evidence supports holding the p44-associated core and CAK module together. Refine this structural role to GO:0140378 protein complex scaffold activity. This does not claim transient recruitment of a particular kinase substrate, kinase catalysis by XPD, or equivalent function of the 405-residue natural product. Proposed replacements: protein complex scaffold activity |
| GO:0005515 protein binding | IPI PMID:20797633 MMXD, a TFIIH-independent XPD-MMS19 protein complex involved... | KEEP AS NON CORE | Summary: XPD associates with CIAO2B/MIP18 as an Fe–S maturation client. Reason: The full PMID:20797633 original was not recovered. Independent recombinant human pairwise binding in PMID:23585563 Figure 4E and the WT-XPD coimmunoprecipitation in PMID:28178521 FigureS 2A directly support association with CIAO2B/MIP18. This client interaction does not establish XPD as the Fe–S transfer catalyst or scaffold. Retain supported generic binding as non-core. |
| GO:0005515 protein binding | IPI PMID:23585563 IOP1 protein is an external component of the human cytosolic... | KEEP AS NON CORE | Summary: XPD physically associates with CIAO1 in its Fe–S maturation context. Reason: PMID:23585563 Figures 2B/3E establish human-cell copurification; Figure 4E and the recombinant binding Methods establish XPD association with MMS19, MIP18 and CIAO1. The annotation is supported, but no more specific molecular function for XPD is established by this client-binding interaction. Preserve generic binding as non-core; do not assign the maturation machinery’s catalytic role to its client. Supporting Evidence: |
| GO:0005515 protein binding | IPI PMID:23585563 IOP1 protein is an external component of the human cytosolic... | KEEP AS NON CORE | Summary: XPD physically associates with MMS19 in its Fe–S maturation context. Reason: PMID:23585563 Figures 2B/3E establish human-cell copurification; Figure 4E and the recombinant binding Methods establish XPD association with MMS19, MIP18 and CIAO1. The annotation is supported, but no more specific molecular function for XPD is established by this client-binding interaction. Preserve generic binding as non-core; do not assign the maturation machinery’s catalytic role to its client. Supporting Evidence: |
| GO:0005515 protein binding | IPI PMID:23585563 IOP1 protein is an external component of the human cytosolic... | KEEP AS NON CORE | Summary: XPD physically associates with MMS19 and CIAO2B/MIP18 in its Fe–S maturation context. Reason: PMID:23585563 Figures 2B/3E establish human-cell copurification; Figure 4E and the recombinant binding Methods establish XPD association with MMS19, MIP18 and CIAO1. The annotation is supported, but no more specific molecular function for XPD is established by this client-binding interaction. Preserve generic binding as non-core; do not assign the maturation machinery’s catalytic role to its client. Supporting Evidence: |
| GO:0005515 protein binding | IPI PMID:23585563 IOP1 protein is an external component of the human cytosolic... | KEEP AS NON CORE | Summary: XPD physically associates with CIAO2B/MIP18 in its Fe–S maturation context. Reason: PMID:23585563 Figures 2B/3E establish human-cell copurification; Figure 4E and the recombinant binding Methods establish XPD association with MMS19, MIP18 and CIAO1. The annotation is supported, but no more specific molecular function for XPD is established by this client-binding interaction. Preserve generic binding as non-core; do not assign the maturation machinery’s catalytic role to its client. Supporting Evidence: |
| GO:0005515 protein binding | IPI PMID:23891004 Human CIA2A-FAM96A and CIA2B-FAM96B integrate iron homeostas... | KEEP AS NON CORE | Summary: Human XPD associates with CIAO1 within the CIA client-binding network. Reason: PMID:23891004 Figure 5D and the corresponding Results report human-cell affinity/MudPIT client interactions, but the exact supplementary table row was not recovered. Independently inspected recombinant binding in PMID:23585563 Figure 4E and WT-XPD co-IP in PMID:28178521 FigureS 2A establish this specific association. No client-independent Fe–S assembly activity is inferred. |
| GO:0005515 protein binding | IPI PMID:23891004 Human CIA2A-FAM96A and CIA2B-FAM96B integrate iron homeostas... | KEEP AS NON CORE | Summary: Human XPD associates with CIAO2B/MIP18 within the CIA client-binding network. Reason: PMID:23891004 Figure 5D and the corresponding Results report human-cell affinity/MudPIT client interactions, but the exact supplementary table row was not recovered. Independently inspected recombinant binding in PMID:23585563 Figure 4E and WT-XPD co-IP in PMID:28178521 FigureS 2A establish this specific association. No client-independent Fe–S assembly activity is inferred. |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | UNDECIDED | Summary: The specific ERCC2–GTF2H2C interaction from the proteome-wide screen remains unresolved. Reason: The main PMID:25416956 article was recovered, but the source-specific target-pair supplementary data were not recovered through the available routes. GTF2H2C (Q6P1K8) is distinct from p44/GTF2H2 (Q13888); evidence for the latter cannot silently resolve this pair. Retain uncertainty without declaring the screen result false or misattributed. Supporting Evidence: |
| GO:0005515 protein binding | IPI PMID:27193682 Near-atomic resolution visualization of human transcription ... | KEEP AS NON CORE | Summary: XPD and XPB are associated within the human TFIIH assembly. Reason: PMID:27193682 Figure 3f and Methods place both proteins in TFIIH immunopurified from HeLa nuclei. This supports complex association, and independent human XPD-GFP/XPB co-IP in PMID:16669699 Figure 1D corroborates it. The structural proximity/complex evidence is not used to invent a pair-specific adaptor activity. |
| GO:0005515 protein binding | IPI PMID:27193682 Near-atomic resolution visualization of human transcription ... | MODIFY | Summary: The XPD–p44 subcomplex contributes to the architectural connection of XPD with TFIIH. Reason: PMID:27193682 Figure 3f and the TFIIH modelling Results resolve the XPD–p44 side of the human TFIIH assembly, with local-resolution and homology-modelling limitations. Independent human interaction/complex-function experiments in PMID:19934020 Figures 4–6 and native CAK–ERCC2 reconstitution in PMID:8692842 support the protein-complex scaffold interpretation. Current GO:0030674 explicitly distinguishes integral protein-complex scaffolds from generic adaptors. XPD is an integral TFIIH subunit whose human assembly/reconstitution and interface evidence supports holding the p44-associated core and CAK module together. Refine this structural role to GO:0140378 protein complex scaffold activity. This does not claim transient recruitment of a particular kinase substrate, kinase catalysis by XPD, or equivalent function of the 405-residue natural product. Proposed replacements: protein complex scaffold activity Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005515 protein binding | IPI PMID:28178521 The CIA Targeting Complex Is Highly Regulated and Provides T... | KEEP AS NON CORE | Summary: XPD associates with CIAO1 as a client of the CIA targeting machinery. Reason: PMID:28178521 FigureS 2A directly shows WT XPD recovery with MMS19, CIAO1 and MIP18 in the coexpression/co-IP assay; Figures 4 and6A additionally characterize MMS19 association. The C190S comparison demonstrates retained association despite altered expression. This supports binding, not an XPD Fe–S assembly catalyst function. Retain as non-core. Supporting Evidence: |
| GO:0005515 protein binding | IPI PMID:28178521 The CIA Targeting Complex Is Highly Regulated and Provides T... | KEEP AS NON CORE | Summary: XPD associates with MMS19 as a client of the CIA targeting machinery. Reason: PMID:28178521 FigureS 2A directly shows WT XPD recovery with MMS19, CIAO1 and MIP18 in the coexpression/co-IP assay; Figures 4 and6A additionally characterize MMS19 association. The C190S comparison demonstrates retained association despite altered expression. This supports binding, not an XPD Fe–S assembly catalyst function. Retain as non-core. Supporting Evidence: |
| GO:0005515 protein binding | IPI PMID:28178521 The CIA Targeting Complex Is Highly Regulated and Provides T... | KEEP AS NON CORE | Summary: XPD associates with CIAO2B/MIP18 as a client of the CIA targeting machinery. Reason: PMID:28178521 FigureS 2A directly shows WT XPD recovery with MMS19, CIAO1 and MIP18 in the coexpression/co-IP assay; Figures 4 and6A additionally characterize MMS19 association. The C190S comparison demonstrates retained association despite altered expression. This supports binding, not an XPD Fe–S assembly catalyst function. Retain as non-core. Supporting Evidence: |
| GO:0005515 protein binding | IPI PMID:35512704 Systematic discovery of mutation-directed neo-protein-protei... | UNDECIDED | Summary: The exact ERCC2–BRAF interaction and WT-versus-mutant context remain unresolved. Reason: PMID:35512704 studies differential and mutation-directed protein interactions. Its main article was recovered, but the exact ERCC2–BRAF supplementary record and the relevant allele-specific assay were not accessible. Neither the generic WT interaction nor a mutation-exclusive result is assumed from the title or the paper’s overall design. Supporting Evidence: |
| GO:0005515 protein binding | IPI PMID:7663514 p53 modulation of TFIIH-associated nucleotide excision repai... | UNDECIDED | Summary: The reported XPD–p53 association could not be independently adjudicated at the original assay level. Reason: PMID:7663514 remains an abstract-level publisher preview. The inspected PMID:8675009 apoptosis experiments cite the earlier interaction but are not themselves a binary binding assay. Retain uncertainty about this source-specific interaction and its appropriate specificity, without inferring that the annotation is incorrect. |
| GO:0005515 protein binding | IPI PMID:8652557 Interactions involving the human RNA polymerase II transcrip... | KEEP AS NON CORE | Summary: XPD–XPB complex association is supported independently. Reason: The full PMID:8652557 original was not accessible; its abstract reports human TFIIH interaction assays. Independent full-length human XPD-GFP/XPB co-IP in PMID:16669699 Figure 1D and human XPD/TFIIH analyses in PMID:19934020 Figure 3C establish the interaction in a complex context. No unsupported pair-specific activity is added. |
| GO:0005515 protein binding | IPI PMID:8652557 Interactions involving the human RNA polymerase II transcrip... | KEEP AS NON CORE | Summary: XPD associates with XPG during nucleotide-excision-repair complex assembly. Reason: The full PMID:8652557 original remains inaccessible. Independent human recombinant repair-complex crosslinking in PMID:31253769 Results “XPG contacts TFIIH” places XPG contacts at the XPD FeS/Arch regions. XPG is absent from the final cryo-EM density, so this is crosslink-supported association rather than a visualized binary interface. Retain generic binding as non-core. |
| GO:0005515 protein binding | IPI PMID:8652557 Interactions involving the human RNA polymerase II transcrip... | KEEP AS NON CORE | Summary: XPD and p62/GTF2H1 coassociate in TFIIH. Reason: The full PMID:8652557 paper remains inaccessible. Independent human XPD/TFIIH immunoprecipitation in PMID:19934020 Figures 3C and4 establishes p62 coassociation. This does not by itself establish a unique molecular activity for that contact, so retain supported generic binding as non-core. |
| GO:0005515 protein binding | IPI PMID:8652557 Interactions involving the human RNA polymerase II transcrip... | MODIFY | Summary: XPD–p44 binding contributes to XPD anchoring within TFIIH. Reason: The complete PMID:8652557 interaction paper was not recovered. Independent human XPD/p44 experiments in PMID:19934020 Figure 4D and the associated integrity/transcription assays support a protein-complex scaffold role, reinforced by native CAK–ERCC2 reconstitution in PMID:8692842. Refine to the supported protein-complex scaffold activity without changing the imported partner identity. Current GO:0030674 explicitly distinguishes integral protein-complex scaffolds from generic adaptors. XPD is an integral TFIIH subunit whose human assembly/reconstitution and interface evidence supports holding the p44-associated core and CAK module together. Refine this structural role to GO:0140378 protein complex scaffold activity. This does not claim transient recruitment of a particular kinase substrate, kinase catalysis by XPD, or equivalent function of the 405-residue natural product. Proposed replacements: protein complex scaffold activity |
| GO:0005515 protein binding | IPI PMID:8652557 Interactions involving the human RNA polymerase II transcrip... | KEEP AS NON CORE | Summary: XPD associates with p34/GTF2H3 within the TFIIH complex. Reason: The full PMID:8652557 interaction paper remains inaccessible. The purified human seven-subunit TFIIH in PMID:31253769 Methods/Figure 2 and the human PIC/TFIIH structural organization in PMID:27193682 Figure 3f establish co-membership and association within the same complex. These do not establish a separate specific molecular activity for the p34–XPD contact; retain generic binding with the source-access and complex-association bounds. |
| GO:0005524 ATP binding | IEA GO_REF:0000002 | ACCEPT | Summary: XPD binds ATP as part of its nucleotide-driven motor cycle. Reason: The four imported InterPro memberships are present in canonical human XPD. Human XPD ATP hydrolysis is directly measured separately from helicase activity in PMID:19934020, Figure 5C and ATPase Methods, and the human XPD K48R motor-site perturbation abolishes unwinding in PMID:31253769. ATP binding is thus an experimentally grounded property, not merely inferred from an unspecified helicase-like fold. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005634 nucleus | EXP PMID:23352696 DNA quality control by a lesion sensor pocket of the xeroder... | MODIFY | Summary: Refine the supported nuclear location to nucleoplasm. Reason: The originally cited paper was not recovered in complete operative form, but full-length human XPD-GFP microscopy in PMID:16669699 Figure 2B/D and endogenous human XPD fractionation in PMID:23585563 Figure 2C independently establish nuclear localization. Preserve the original source and retain the supported localization evidence without claiming that its inaccessible Methods were read. The independently inspected official Human Protein Atlas ERCC2 record reports supported nucleoplasmic localization with antibody HPA069266 in SiHa, U-251MG and U2OS cells. Refine the broad nuclear term consistently with the other nuclear annotations to the already represented GO:0005654. Additional cytosolic signal is compatible; the observations are not isoform-resolved and the images were not independently inspected. Proposed replacements: nucleoplasm Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005634 nucleus | IBA GO_REF:0000033 | MODIFY | Summary: Refine the supported nuclear location to nucleoplasm. Reason: The official target Human Protein Atlas record ENSG00000104884 uses antibody HPA069266 and reports supported nucleoplasmic localization in SiHa, U-251MG and U2OS cells; U-251MG also shows cytosolic signal. Independent human full-length XPD-GFP imaging/fractionation establishes nuclear and cytoplasmic pools (PMID:16669699, Figure 2). The refinement identifies an established location without asserting exclusivity, disproving other nuclear compartments or assigning all natural products the same distribution. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000158239 SUPPORTS TRANSFER The saved GO:0005634 IBD is valid for the target; target human localization permits a finer compartment description. The target leaf PTN002479324 descends from this exact node in the official PTHR11472 tree; its aligned sequence matches canonical human P18074 (760 aa), and no NOT record lies on its saved IBD target path. Proposed replacements: nucleoplasm Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005634 nucleus | IDA PMID:20797633 MMXD, a TFIIH-independent XPD-MMS19 protein complex involved... | MODIFY | Summary: Refine the supported nuclear location to nucleoplasm. Reason: The originally cited paper was not recovered in complete operative form, but full-length human XPD-GFP microscopy in PMID:16669699 Figure 2B/D and endogenous human XPD fractionation in PMID:23585563 Figure 2C independently establish nuclear localization. Preserve the original source and retain the supported localization evidence without claiming that its inaccessible Methods were read. The independently inspected official Human Protein Atlas ERCC2 record reports supported nucleoplasmic localization with antibody HPA069266 in SiHa, U-251MG and U2OS cells. Refine the broad nuclear term consistently with the other nuclear annotations to the already represented GO:0005654. Additional cytosolic signal is compatible; the observations are not isoform-resolved and the images were not independently inspected. Proposed replacements: nucleoplasm Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005634 nucleus | IDA PMID:23585563 IOP1 protein is an external component of the human cytosolic... | MODIFY | Summary: Refine the supported nuclear location to nucleoplasm. Reason: PMID:23585563 Figure 2C and the subcellular-fractionation Methods directly establish predominantly nuclear XPD in WT and MMS19-depleted human cells. This is consistent with its TFIIH repair/transcription function; it does not exclude a cytoplasmic pool. The independently inspected official Human Protein Atlas ERCC2 record reports supported nucleoplasmic localization with antibody HPA069266 in SiHa, U-251MG and U2OS cells. Refine the broad nuclear term consistently with the other nuclear annotations to the already represented GO:0005654. Additional cytosolic signal is compatible; the observations are not isoform-resolved and the images were not independently inspected. Proposed replacements: nucleoplasm |
| GO:0005634 nucleus | IEA GO_REF:0000120 | MODIFY | Summary: Refine the supported nuclear location to nucleoplasm. Reason: The official target Human Protein Atlas record ENSG00000104884 uses antibody HPA069266 and reports supported nucleoplasmic localization in SiHa, U-251MG and U2OS cells; U-251MG also shows cytosolic signal. Independent human full-length XPD-GFP imaging/fractionation establishes nuclear and cytoplasmic pools (PMID:16669699, Figure 2). The refinement identifies an established location without asserting exclusivity, disproving other nuclear compartments or assigning all natural products the same distribution. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:O08811 SUPPORTS TRANSFER Mouse nuclear IDA is traceable to PMID:17952069; that original remains preview-only here. Independent target HPA and human XPD-GFP localization resolve the human compartment. ensembl:ENSMUSP00000054380 SUPPORTS TRANSFER Official mouse record identifies the imported ortholog; no target-versus-donor identity failure is present. InterPro:IPR001945 SUPPORTS TRANSFER Official target domain/family membership and nuclear GO mapping are present. UniProtKB-SubCell:SL-0191 SUPPORTS TRANSFER Official nuclear localization vocabulary supports the broad source claim; the target HPA record supplies nucleoplasmic specificity. Proposed replacements: nucleoplasm Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | IDA GO_REF:0000052 | ACCEPT | Summary: The target HPA assay supports ERCC2 in nucleoplasm. Reason: The actual official HPA ERCC2 subcellular page and XML identify antibody HPA069266, supported nucleoplasmic localization and assays in SiHa, U-251MG and U2OS human cells. This source was read as structured assay/localization data; the fluorescence images were not independently inspected. The record is not isoform-resolved. Human XPD-GFP localization independently supports a nuclear pool (PMID:16669699, Figure 2). Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-109639 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 109639 (Formation of the closed pre-initiation complex) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 109639 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-109639 SUPPORTS TRANSFER Official forward chain 109639 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-111264 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 111264 (Addition of nucleotides between position +11 and +30) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 111264 → 75902 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-111264 SUPPORTS TRANSFER Official forward chain 111264 → 75902 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-112379 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 112379 (Recruitment of elongation factors to form elongation complex) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 112379 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-112379 SUPPORTS TRANSFER Official forward chain 112379 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-112383 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 112383 (Hypophosphorylation of RNA Pol II CTD by FCP1P protein) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 112383 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-112383 SUPPORTS TRANSFER Official forward chain 112383 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-112385 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 112385 (Addition of nucleotides leads to transcript elongation) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 112385 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-112385 SUPPORTS TRANSFER Official forward chain 112385 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-113430 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 113430 (Extrusion of 5'-end of 30 nt long transcript through the pore in Pol II complex) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 113430 → 111261 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-113430 SUPPORTS TRANSFER Official forward chain 113430 → 111261 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-5689317 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 5689317 (Formation of the pre-incision complex in GG-NER) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 5689317 → 6790517 → 5689624 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-5689317 SUPPORTS TRANSFER Official forward chain 5689317 → 6790517 → 5689624 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-5689861 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 5689861 (Recruitment of XPA and release of CAK ) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 5689861 → 5689862 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-5689861 SUPPORTS TRANSFER Official forward chain 5689861 → 5689862 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-5690213 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 5690213 (DNA polymerases delta, epsilon or kappa bind the GG-NER site) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 5690213 → 5691059 → 5689624 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-5690213 SUPPORTS TRANSFER Official forward chain 5690213 → 5691059 → 5689624 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-5690988 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 5690988 (3'-incision of DNA by ERCC5 (XPG) in GG-NER) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 5690988 → 5689624 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-5690988 SUPPORTS TRANSFER Official forward chain 5690988 → 5689624 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-5690990 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 5690990 (5'- incision of DNA by ERCC1:ERCC4 in GG-NER) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 5690990 → 5691059 → 5689624 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-5690990 SUPPORTS TRANSFER Official forward chain 5690990 → 5691059 → 5689624 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-5690991 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 5690991 (Binding of ERCC1:ERCC4 (ERCC1:XPF) to pre-incision complex in GG-NER) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 5690991 → 5691043 → 6790517 → 5689624 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-5690991 SUPPORTS TRANSFER Official forward chain 5690991 → 5691043 → 6790517 → 5689624 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-5690996 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 5690996 (ERCC2 and ERCC3 DNA helicases form an open bubble structure in damaged DNA) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 5690996 → 5691018 → 5689624 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-5690996 SUPPORTS TRANSFER Official forward chain 5690996 → 5691018 → 5689624 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-5691000 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 5691000 (TFIIH binds GG-NER site to form a verification complex) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 5691000 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-5691000 SUPPORTS TRANSFER Official forward chain 5691000 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-5696670 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 5696670 (CHD1L is recruited to GG-NER site) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 5696670 → 5689300 → 5689624 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-5696670 SUPPORTS TRANSFER Official forward chain 5696670 → 5689300 → 5689624 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6781818 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 6781818 (RNA Pol II initiates transcription from damaged DNA template) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 6781818 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-6781818 SUPPORTS TRANSFER Official forward chain 6781818 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6781824 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 6781824 (Active RNA Pol II complex transcribes lesion-containing DNA template) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 6781824 → 6781821 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-6781824 SUPPORTS TRANSFER Official forward chain 6781824 → 6781821 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6781833 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 6781833 (ERCC8 (CSA) binds stalled RNA Pol II) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 6781833 → 6781837 → 6781822 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-6781833 SUPPORTS TRANSFER Official forward chain 6781833 → 6781837 → 6781822 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6781840 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 6781840 (ERCC6 binds stalled RNA Pol II) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 6781840 → 6781822 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-6781840 SUPPORTS TRANSFER Official forward chain 6781840 → 6781822 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6781867 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 6781867 (ERCC8:DDB1:CUL4:RBX1 ubiquitinates ERCC6 and RNA Pol II) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 6781867 → 6781839 → 6781837 → 6781822 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-6781867 SUPPORTS TRANSFER Official forward chain 6781867 → 6781839 → 6781837 → 6781822 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6782004 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 6782004 (Assembly of the pre-incision complex in TC-NER) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 6782004 → 6781866 → 6781848 → 6781861 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-6782004 SUPPORTS TRANSFER Official forward chain 6782004 → 6781866 → 6781848 → 6781861 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6782069 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 6782069 (UVSSA:USP7 deubiquitinates ERCC6) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 6782069 → 6782063 → 6781866 → 6781848 → 6781861 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-6782069 SUPPORTS TRANSFER Official forward chain 6782069 → 6782063 → 6781866 → 6781848 → 6781861 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6782131 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 6782131 (ERCC2-facilitated RNA Pol II backtracking in TC-NER) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 6782131 → 6782120 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-6782131 SUPPORTS TRANSFER Official forward chain 6782131 → 6782120 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6782138 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 6782138 (ERCC5 and RPA bind TC-NER site) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 6782138 → 6782111 → 6782122 → 6782112 → 6782116 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-6782138 SUPPORTS TRANSFER Official forward chain 6782138 → 6782111 → 6782122 → 6782112 → 6782116 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6782141 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 6782141 (Binding of ERCC1:ERCC4 (ERCC1:XPF) to pre-incision complex in TC-NER) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 6782141 → 6782134 → 6782111 → 6782122 → 6782112 → 6782116 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-6782141 SUPPORTS TRANSFER Official forward chain 6782141 → 6782134 → 6782111 → 6782122 → 6782112 → 6782116 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6782204 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 6782204 (5' incision of damaged DNA strand by ERCC1:ERCC4 in TC-NER) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 6782204 → 6782205 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-6782204 SUPPORTS TRANSFER Official forward chain 6782204 → 6782205 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6782208 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 6782208 (Repair DNA synthesis of ~27-30 bases long patch by POLD, POLE or POLK in TC-NER) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 6782208 → 6782222 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-6782208 SUPPORTS TRANSFER Official forward chain 6782208 → 6782222 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6782211 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 6782211 (DNA polymerases delta, epsilon or kappa bind the TC-NER site) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 6782211 → 6782205 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-6782211 SUPPORTS TRANSFER Official forward chain 6782211 → 6782205 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6782224 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 6782224 (3' incision by ERCC5 (XPG) in TC-NER) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 6782224 → 6782219 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-6782224 SUPPORTS TRANSFER Official forward chain 6782224 → 6782219 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6782227 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 6782227 (Ligation of newly synthesized repair patch to incised DNA in TC-NER) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 6782227 → 6782220 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-6782227 SUPPORTS TRANSFER Official forward chain 6782227 → 6782220 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6782234 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 6782234 (Recovery of RNA synthesis after TC-NER) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 6782234 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-6782234 SUPPORTS TRANSFER Official forward chain 6782234 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6790454 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 6790454 (SUMOylation of XPC) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 6790454 → 5691018 → 5689624 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-6790454 SUPPORTS TRANSFER Official forward chain 6790454 → 5691018 → 5689624 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6790487 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 6790487 (RNF111 ubiquitinates SUMOylated XPC) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 6790487 → 6790468 → 5689624 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-6790487 SUPPORTS TRANSFER Official forward chain 6790487 → 6790468 → 5689624 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6797606 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 6797606 (CDK12 phosphorylates RNA Pol II CTD at DNA repair genes) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 6797606 → 6797614 → 6797611 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-6797606 SUPPORTS TRANSFER Official forward chain 6797606 → 6797614 → 6797611 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6797616 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 6797616 (CCNK:CDK12 binds RNA Pol II at DNA repair genes) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 6797616 → 6797611 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-6797616 SUPPORTS TRANSFER Official forward chain 6797616 → 6797611 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-73758 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 73758 (Recruitment of RNA Polymerase I:RRN3 to the 45S pre-rRNA gene) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 73758 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-73758 SUPPORTS TRANSFER Official forward chain 73758 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-73769 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 73769 (Loss of RRN3 from RNA Polymerase I promoter escape complex) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 73769 → 73716 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-73769 SUPPORTS TRANSFER Official forward chain 73769 → 73716 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-73946 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 73946 (Abortive initiation after formation of the first phosphodiester bond) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 73946 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-73946 SUPPORTS TRANSFER Official forward chain 73946 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-74986 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 74986 (Elongation of pre-rRNA transcript) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 74986 → 73717 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-74986 SUPPORTS TRANSFER Official forward chain 74986 → 73717 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-74992 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 74992 (Dissociation of PTRF:RNA Polymerase I:pre-rRNA complex) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 74992 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-74992 SUPPORTS TRANSFER Official forward chain 74992 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-74993 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 74993 (PTRF binds the paused RNA Polymerase I complex) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 74993 → 74979 → 73717 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-74993 SUPPORTS TRANSFER Official forward chain 74993 → 74979 → 73717 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-74994 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 74994 (Elongating RNA Polymerase I Transcription Complex pauses at the TTF1:Sal Box) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 74994 → 9988366 → 73717 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-74994 SUPPORTS TRANSFER Official forward chain 74994 → 9988366 → 73717 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75850 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 75850 (Addition of the third nucleotide on the nascent transcript) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 75850 → 109878 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-75850 SUPPORTS TRANSFER Official forward chain 75850 → 109878 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75856 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 75856 (Abortive Initiation Before Second Transition) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 75856 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-75856 SUPPORTS TRANSFER Official forward chain 75856 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75861 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 75861 (NTP Binds Active Site of RNA Polymerase II) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 75861 → 109876 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-75861 SUPPORTS TRANSFER Official forward chain 75861 → 109876 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75862 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 75862 (Fall Back to Closed Pre-initiation Complex) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 75862 → 109876 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-75862 SUPPORTS TRANSFER Official forward chain 75862 → 109876 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75864 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 75864 (Newly Formed Phosphodiester Bond Stabilized and PPi Released) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 75864 → 83601 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-75864 SUPPORTS TRANSFER Official forward chain 75864 → 83601 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75866 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 75866 (Nucleophillic Attack by 3'-hydroxyl Oxygen of nascent transcript on the Alpha Phosphate of NTP) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 75866 → 83601 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-75866 SUPPORTS TRANSFER Official forward chain 75866 → 83601 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75869 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 75869 (Addition of the fourth nucleotide on the Nascent Transcript: Second Transition) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 75869 → 75878 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-75869 SUPPORTS TRANSFER Official forward chain 75869 → 75878 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75873 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 75873 (Addition of Nucleotides 5 through 9 on the growing Transcript) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 75873 → 75881 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-75873 SUPPORTS TRANSFER Official forward chain 75873 → 75881 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75891 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 75891 (Abortive Initiation After Second Transition) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 75891 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-75891 SUPPORTS TRANSFER Official forward chain 75891 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75949 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 75949 (RNA Polymerase II Promoter Opening: First Transition) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 75949 → 109635 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-75949 SUPPORTS TRANSFER Official forward chain 75949 → 109635 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-76576 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 76576 (Addition of nucleotides 10 and 11 on the growing transcript: Third Transition) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 76576 → 75882 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-76576 SUPPORTS TRANSFER Official forward chain 76576 → 75882 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77068 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 77068 (Activation of GT) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 77068 → 77053 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-77068 SUPPORTS TRANSFER Official forward chain 77068 → 77053 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77069 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 77069 (RNA Polymerase II CTD (phosphorylated) binds to CE) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 77069 → 157174 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-77069 SUPPORTS TRANSFER Official forward chain 77069 → 157174 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77071 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 77071 (Phosphorylation (Ser5) of RNA pol II CTD) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 77071 → 157171 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-77071 SUPPORTS TRANSFER Official forward chain 77071 → 157171 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77073 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 77073 (SPT5 subunit of Pol II binds the RNA triphosphatase (RTP)) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 77073 → 77056 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-77073 SUPPORTS TRANSFER Official forward chain 77073 → 77056 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77077 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 77077 (Capping complex formation) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 77077 → 77061 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-77077 SUPPORTS TRANSFER Official forward chain 77077 → 77061 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77078 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 77078 (Hydrolysis of the 5'-end of the nascent transcript by the capping enzyme) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 77078 → 77063 → 77061 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-77078 SUPPORTS TRANSFER Official forward chain 77078 → 77063 → 77061 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77081 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 77081 (Formation of the CE:GMP intermediate complex) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 77081 → 77064 → 77061 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-77081 SUPPORTS TRANSFER Official forward chain 77081 → 77064 → 77061 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77083 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 77083 (Transfer of GMP from the capping enzyme GT site to 5'-end of mRNA) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 77083 → 77066 → 77061 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-77083 SUPPORTS TRANSFER Official forward chain 77083 → 77066 → 77061 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77085 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 77085 (Dissociation of transcript with 5'-GMP from GT) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 77085 → 77066 → 77061 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-77085 SUPPORTS TRANSFER Official forward chain 77085 → 77066 → 77061 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77090 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 77090 (Methylation of GMP-cap by RNA Methyltransferase) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 77090 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-77090 SUPPORTS TRANSFER Official forward chain 77090 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9613494 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 9613494 (Unwinding of DNA for the Nascent Transcript: Second Transition) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 9613494 → 75878 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-9613494 SUPPORTS TRANSFER Official forward chain 9613494 → 75878 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9613497 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 9613497 (Unwinding DNA for the nascent transcript) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 9613497 → 109878 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-9613497 SUPPORTS TRANSFER Official forward chain 9613497 → 109878 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9684118 | ACCEPT | Summary: The Reactome source explicitly contains nucleoplasmic human ERCC2. Reason: The current official source 9684118 (ERCC3-facilitated RNA Pol II backtracking in TC-NER) reaches ERCC2 physical entity R-HSA-67443 through the saved forward participant/component chain 9684118 → 9684117 → 9684121 → 9684120 → 9684119 → 109634 → 67443. That entity has reference P18074, coordinates 1–760 and its own GO:0005654 compartment. Thus the location assertion is traceable to the target protein rather than only the event compartment. Independent HPA target localization and human XPD-GFP nuclear localization agree (PMID:16669699, Figure 2). Membership in this event does not make ERCC2 the polymerase, nuclease, capping enzyme, kinase or ubiquitin ligase named in the surrounding reaction, and does not prove activity of the 405-aa product. Propagation Review Root cause: NO FAILURE CORE Sources checked: Reactome:R-HSA-9684118 SUPPORTS TRANSFER Official forward chain 9684118 → 9684117 → 9684121 → 9684120 → 9684119 → 109634 → 67443 reaches P18074 entity67443 with its own nucleoplasmic compartment; no event-to-protein role transfer is inferred. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0005669 transcription factor TFIID complex | IDA PMID:27193682 Near-atomic resolution visualization of human transcription ... | MODIFY | Summary: The source places XPD in TFIIH, not in the TFIID complex. Reason: The actual PMID:27193682 Results explicitly use TBP in place of TFIID, while Methods immunopurify TFIIH from HeLa nuclei. Figure 3f/Extended Data 6 locate XPD within TFIIH. Current GO:0005669 specifically denotes the TBP–TAF complex, whereas GO:0000439 explicitly includes XPD in the seven-subunit TFIIH core. Replace this complex assignment using the source’s actual preparations and current definitions, not the article title. Proposed replacements: transcription factor TFIIH core complex Supporting Evidence: |
| GO:0005675 transcription factor TFIIH holo complex | IDA PMID:9852112 Immunoaffinity purification and functional characterization ... | ACCEPT | Summary: XPD is a component of the transcriptionally competent human TFIIH holo complex. Reason: The complete originally cited article was not recovered. Independent native human TFIIH purification in PMID:8692842 Figure 1 and recombinant human XPD/TFIIH interaction and transcription assays in PMID:19934020 Figures 3–6 directly support membership of the holo complex. The CDK7 kinase activity of that complex is not attributed to XPD itself. |
| GO:0005675 transcription factor TFIIH holo complex | IPI PMID:15220921 A new, tenth subunit of TFIIH is responsible for the DNA rep... | ACCEPT | Summary: XPD is a component of the transcriptionally competent human TFIIH holo complex. Reason: The complete originally cited article was not recovered. Independent native human TFIIH purification in PMID:8692842 Figure 1 and recombinant human XPD/TFIIH interaction and transcription assays in PMID:19934020 Figures 3–6 directly support membership of the holo complex. The CDK7 kinase activity of that complex is not attributed to XPD itself. |
| GO:0005675 transcription factor TFIIH holo complex | TAS PMID:7663514 p53 modulation of TFIIH-associated nucleotide excision repai... | ACCEPT | Summary: XPD is a component of the transcriptionally competent human TFIIH holo complex. Reason: The complete originally cited article was not recovered. Independent native human TFIIH purification in PMID:8692842 Figure 1 and recombinant human XPD/TFIIH interaction and transcription assays in PMID:19934020 Figures 3–6 directly support membership of the holo complex. The CDK7 kinase activity of that complex is not attributed to XPD itself. |
| GO:0005737 cytoplasm | IDA PMID:20797633 MMXD, a TFIIH-independent XPD-MMS19 protein complex involved... | KEEP AS NON CORE | Summary: A human XPD pool is present in the cytoplasm in addition to nuclear TFIIH. Reason: Full-length human XPD-GFP microscopy and intact-protein immunoblotting in PMID:16669699 Figure 2B/D establish a cytoplasmic pool. PMID:23585563 Figure 2C emphasizes predominantly nuclear endogenous XPD, so its wording is not treated as proof of exclusive localization. Retain the cytoplasmic location as a non-core pool associated with maturation/alternative assemblies, without assigning every molecule to MMXD. |
| GO:0005737 cytoplasm | IDA PMID:23585563 IOP1 protein is an external component of the human cytosolic... | KEEP AS NON CORE | Summary: A human XPD pool is present in the cytoplasm in addition to nuclear TFIIH. Reason: Full-length human XPD-GFP microscopy and intact-protein immunoblotting in PMID:16669699 Figure 2B/D establish a cytoplasmic pool. PMID:23585563 Figure 2C emphasizes predominantly nuclear endogenous XPD, so its wording is not treated as proof of exclusive localization. Retain the cytoplasmic location as a non-core pool associated with maturation/alternative assemblies, without assigning every molecule to MMXD. |
| GO:0005819 spindle | IDA PMID:20797633 MMXD, a TFIIH-independent XPD-MMS19 protein complex involved... | UNDECIDED | Summary: The reported mitotic-spindle localization of XPD remains source-specific uncertainty. Reason: PMID:20797633 explicitly reports spindle localization in its abstract, but the original microscopy, antibodies and cell/construct details were not recovered. Independently established nuclear/cytoplasmic localization does not resolve spindle enrichment. Preserve uncertainty rather than reject a curator-supported observation. Supporting Evidence: |
| GO:0005819 spindle | IEA GO_REF:0000044 | UNDECIDED | Summary: A mitotic spindle assignment is plausible but its source-specific localization evidence remains inaccessible. Reason: The imported UniProt spindle vocabulary SL-0251 is valid, and target UniProt traces the MMXD/spindle claim to PMID:20797633. The source abstract is positive, but the operative original localization assays and their timing/construct scope have not been recovered in this lane. This is not contradicted by the independently established nuclear and cytoplasmic pools. Retain uncertainty rather than infer a constitutive spindle function or reject the annotation from incomplete access. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0006283 transcription-coupled nucleotide-excision repair | IDA PMID:8663148 A 3' --> 5' XPB helicase defect in repair/transcription fact... | ACCEPT | Summary: XPD contributes its TFIIH repair activity to transcription-coupled nucleotide-excision repair. Reason: The complete PMID:8663148 original was not recovered, so its exact XPD experiment is not claimed as inspected. Independently verified current human Reactome pathway R-HSA-6781827 explicitly includes full-length XPD in the shared TFIIH pre-incision machinery and the XPD motor event. Human XPD/TFIIH repair and unwinding experiments in PMID:19934020 Figure 5 and PMID:31253769 Figure 1 independently establish the chemistry by which XPD contributes. Accept the established pathway participation on this combined basis, without inferring original-specific verification from an XPB-focused abstract or treating every Reactome RNA-polymerase-backtracking model as directly demonstrated. Supporting Evidence: Reactome:R-HSA-6781827 file:human/ERCC2/ERCC2-source-guide.md |
| GO:0006283 transcription-coupled nucleotide-excision repair | TAS Reactome:R-HSA-6781827 | ACCEPT | Summary: ERCC2 contributes its TFIIH repair activity to transcription-coupled nucleotide-excision repair. Reason: Reactome pathway R-HSA-6781827 contains the pre-incision and XPD motor events and traces to human P18074 entity67443. ERCC2 performs an actual DNA-opening step in the shared NER machinery; it is not simply a damaged substrate or a repair-response marker. Independently read human XPD/TFIIH reconstitution establishes the ATP-dependent helicase and repair contribution (PMID:19934020, Figure 5; PMID:31253769, Results/Figures 1–4). The lesion-free structure does not independently demonstrate every proposed RNA-polymerase-backtracking mechanism in this pathway. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0006289 nucleotide-excision repair | IBA GO_REF:0000033 | ACCEPT | Summary: XPD performs the DNA-opening work of nucleotide-excision repair. Reason: The exact PTN000158239 NER IBD and human target descent are verified. Human XPD mutant/core-TFIIH experiments directly measure lesion incision and DNA unwinding (PMID:19934020, Figure 5 and reconstituted NER Methods). Human core-TFIIH structures and motor assays explain its repair-bubble function (PMID:31253769, Figures 1–4). This is execution of a repair step, distinct from kinase activity and from the catalytic incision performed by other NER enzymes. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000158239 SUPPORTS TRANSFER The exact GO:0006289 IBD supports inherited NER participation; direct human repair reconstitution independently establishes target participation. The target leaf PTN002479324 descends from this exact node in the official PTHR11472 tree; its aligned sequence matches canonical human P18074 (760 aa), and no NOT record lies on its saved IBD target path. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0006289 nucleotide-excision repair | IEA GO_REF:0000002 | ACCEPT | Summary: XPD performs the DNA-opening work of nucleotide-excision repair. Reason: The IPR001945 target membership and its NER mapping are verified. Human XPD mutant/core-TFIIH experiments directly measure lesion incision and DNA unwinding (PMID:19934020, Figure 5 and reconstituted NER Methods). Human core-TFIIH structures and motor assays explain its repair-bubble function (PMID:31253769, Figures 1–4). This is execution of a repair step, distinct from kinase activity and from the catalytic incision performed by other NER enzymes. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0006289 nucleotide-excision repair | IMP PMID:8692841 Isolation and characterization of two human transcription fa... | ACCEPT | Summary: Human ERCC2/XPD supports nucleotide-excision repair. Reason: PMID:8692841 Figure 5 complements XPD-defective hamster-cell extracts with purified human ERCC2/CAK fractions on a cholesterol-lesion substrate. Independent human recombinant TFIIH assays in PMID:19934020 Figure 5 and PMID:31253769 Figure 1 establish the unwinding contribution. The excision nucleases are other components; XPD is not assigned incision catalysis. |
| GO:0006289 nucleotide-excision repair | NAS PMID:2184031 ERCC2: cDNA cloning and molecular characterization of a huma... | ACCEPT | Summary: Human ERCC2 contributes to nucleotide-excision repair and UV resistance. Reason: PMID:2184031 Results/Figure 3 and complementation Methods introduce human genomic/cDNA sequences into repair-defective hamster UV5 cells. Partial cDNA and genomic constructs differ in stability of rescue, so they are not equated with either natural isoform. Independent human biochemical evidence in PMID:19934020 Figure 5 resolves the core repair function. |
| GO:0006289 nucleotide-excision repair | NAS PMID:22572993 TFIIH: when transcription met DNA repair. | ACCEPT | Summary: XPD is an ATP-dependent DNA-unwinding component of nucleotide-excision repair. Reason: The complete PMID:22572993 review was not accessible. Independent original human reconstitution/repair assays in PMID:19934020 Figure 5 and PMID:31253769 Figure 1/Supplementary Figure 1 directly establish the function. Retain the source row while identifying the independent experimental basis. |
| GO:0006357 regulation of transcription by RNA polymerase II | IDA PMID:8692842 Human cyclin-dependent kinase-activating kinase exists in th... | MODIFY | Summary: The human CAK–ERCC2 assembly contributes specifically to RNA-polymerase-II transcription initiation. Reason: PMID:8692842 Figure 6 reconstitutes transcription with CAK–ERCC2 and depleted TFIIH, while Figure 5 separates kinase-module substrate effects. The demonstrated XPD role is organization of the initiation machinery, already represented by GO:0006367, rather than modulation of transcription as a separate regulatory process. Replace the regulation assertion with participation in transcription initiation. This corrects the biological role across distinct process branches; GO:0006367 is not a descendant refinement of GO:0006357. Proposed replacements: transcription initiation at RNA polymerase II promoter Supporting Evidence: |
| GO:0006366 transcription by RNA polymerase II | IBA GO_REF:0000033 | MODIFY | Summary: Refine the supported TFIIH contribution to RNA-polymerase-II transcription initiation. Reason: The GO:0006366 IBD at PTN000158239 includes target human XPD experimental grounding and valid target descent. Human recombinant TFIIH with XPD variants was assayed in a reconstituted adenovirus major-late-promoter transcription system (PMID:19934020, Figure 6A and transcription Methods). These experiments support a TFIIH structural/assembly contribution rather than XPD synthesis of RNA or a claim that XPD helicase catalysis drives every transcription reaction. Self-presence in the descendant evidence list is expected and is not circular propagation. Native human CAK–ERCC2 reconstitution (PMID:8692842, Figure 6) and the human initiation-complex structure (PMID:27193682, Figure 3) identify the initiation contribution more specifically. Refine to the already represented GO:0006367 without rejecting the valid ancestral broader assertion or assigning RNA synthesis to XPD. Propagation Review Root cause: TERM SCOPING PROBLEM Sources checked: PANTHER:PTN000158239 SUPPORTS TRANSFER The exact GO:0006366 IBD incorporates human experimental evidence; actual human reconstituted transcription supports the TFIIH contribution without assigning RNA polymerase catalysis to XPD. The target leaf PTN002479324 descends from this exact node in the official PTHR11472 tree; its aligned sequence matches canonical human P18074 (760 aa), and no NOT record lies on its saved IBD target path. Proposed replacements: transcription initiation at RNA polymerase II promoter Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0006366 transcription by RNA polymerase II | IDA PMID:9852112 Immunoaffinity purification and functional characterization ... | MODIFY | Summary: XPD supports RNA-polymerase-II transcription initiation through TFIIH architecture. Reason: The complete originally cited paper was not recovered. Independent human TFIIH/CAK–ERCC2 reconstitution in PMID:8692842 Figure 6, human XPD-dependent transcription assays in PMID:19934020 Figure 6, and the human initiation-complex structure in PMID:27193682 establish the more specific initiation role. Refine the broad transcription term to the already represented GO:0006367 without assigning RNA synthesis or XPB translocase activity to XPD. Proposed replacements: transcription initiation at RNA polymerase II promoter |
| GO:0006366 transcription by RNA polymerase II | TAS PMID:8663148 A 3' --> 5' XPB helicase defect in repair/transcription fact... | MODIFY | Summary: XPD supports RNA-polymerase-II transcription initiation through TFIIH architecture. Reason: The complete originally cited paper was not recovered. Independent human TFIIH/CAK–ERCC2 reconstitution in PMID:8692842 Figure 6, human XPD-dependent transcription assays in PMID:19934020 Figure 6, and the human initiation-complex structure in PMID:27193682 establish the more specific initiation role. Refine the broad transcription term to the already represented GO:0006367 without assigning RNA synthesis or XPB translocase activity to XPD. Proposed replacements: transcription initiation at RNA polymerase II promoter |
| GO:0006367 transcription initiation at RNA polymerase II promoter | IDA PMID:8692841 Isolation and characterization of two human transcription fa... | ACCEPT | Summary: Human XPD contributes to transcription initiation through TFIIH assembly. Reason: PMID:8692841 Figure 4 uses a reconstituted RNA-polymerase-II transcription assay: ERCC2/CAK stimulates the depleted TFIIH fraction but does not substitute for the entire complex. PMID:8692842 Figure 6 independently supports this architectural contribution. XPD need not synthesize RNA or hydrolyze ATP during this step to contribute the required structure. |
| GO:0006367 transcription initiation at RNA polymerase II promoter | NAS PMID:22572993 TFIIH: when transcription met DNA repair. | ACCEPT | Summary: XPD has a structural contribution to RNA-polymerase-II initiation. Reason: The complete PMID:22572993 review was not recovered; independent original human CAK–ERCC2/TFIIH reconstitution in PMID:8692842 Figure 6 and human XPD/TFIIH transcription assays in PMID:19934020 Figure 6 establish this role. Distinguish the structural contribution from XPD’s repair helicase activity. |
| GO:0006915 apoptotic process | IMP PMID:8675009 The XPB and XPD DNA helicases are components of the p53-medi... | KEEP AS NON CORE | Summary: XPD supports the p53-induced apoptotic response in the tested human fibroblast context. Reason: PMID:8675009 Results pages 1221–1224, Table 3/Figure 4B and Methods pages 1228–1229 show impaired p53-induced apoptosis in XP-D fibroblasts and rescue with wild-type XPD cDNA; p21 induction is retained. This supports a contextual contribution, while the exact downstream molecular mechanism is unresolved. It is not a universal death-execution function and is retained as non-core. Supporting Evidence: |
| GO:0006979 response to oxidative stress | IMP PMID:17614221 Genetic susceptibility of newborn daughters to oxidative str... | UNDECIDED | Summary: The XPD-specific oxidative-stress claim remains unresolved. Reason: PMID:17614221 is a human mother–newborn genotype/oxidative-damage study, but only the verified abstract was accessible. The original genotype-stratified data and XPD-specific result are needed to adjudicate this imported IMP assertion. Do not convert the study’s overall oxidative-stress setting into an established XPD response mechanism or reject the curator’s row from the abstract alone. Supporting Evidence: |
| GO:0007059 chromosome segregation | IMP PMID:20797633 MMXD, a TFIIH-independent XPD-MMS19 protein complex involved... | UNDECIDED | Summary: The reported contribution to chromosome segregation requires the original MMXD experiments. Reason: PMID:20797633 reports knockdown and patient-cell mitotic phenotypes, but the full operative original was not recovered. Other inspected CIA-client studies do not resolve the specific XPD segregation mechanism or controls. Preserve source-specific uncertainty without asserting that perturbation evidence is intrinsically invalid for a cellular process. Supporting Evidence: |
| GO:0016818 hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides | IEA GO_REF:0000002 | MODIFY | Summary: Refine the broad phosphorus-anhydride hydrolase annotation to ATP hydrolysis. Reason: The InterPro-mapped catalytic domains are consistent with ATP hydrolysis. Independently inspected human XPD preparations in PMID:19934020 Figure 5C and the ATPase Methods directly release phosphate from ATP after high-salt washing. Mutants can retain ATPase activity while losing productive DNA unwinding, so these are separable measured functions. GO:0016887 ATP hydrolysis activity is an is_a descendant of this broad hydrolase term and preserves the asserted chemistry. The separately supported 5′–3′ helicase activity remains represented by other annotations. Proposed replacements: ATP hydrolysis activity Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0016887 ATP hydrolysis activity | EXP PMID:8413672 Human xeroderma pigmentosum group D gene encodes a DNA helic... | ACCEPT | Summary: Human XPD directly hydrolyzes ATP; this activity is experimentally separable from productive DNA unwinding. Reason: The complete originally cited Nature article was not recovered. Independent original human XPD experiments in PMID:19934020 Figure 5C and the ATPase Methods directly measure phosphate release from radiolabeled ATP in high-salt-washed immunoprecipitated XPD preparations. The Results distinguish retained ATP hydrolysis in the tested variants from impaired helicase activity. This supports retaining the established ATP-hydrolysis annotation on independent evidence, without claiming full verification of the inaccessible original. The 5′–3′ DNA-helicase annotations describe the separately tested unwinding function; they do not replace this chemistry or transfer XPB translocation or CDK7 kinase activity to XPD. |
| GO:0016887 ATP hydrolysis activity | EXP PMID:9771713 Mutations in the XPD helicase gene result in XP and TTD phen... | ACCEPT | Summary: Human XPD directly hydrolyzes ATP; this activity is experimentally separable from productive DNA unwinding. Reason: The complete originally cited Nature article was not recovered. Independent original human XPD experiments in PMID:19934020 Figure 5C and the ATPase Methods directly measure phosphate release from radiolabeled ATP in high-salt-washed immunoprecipitated XPD preparations. The Results distinguish retained ATP hydrolysis in the tested variants from impaired helicase activity. This supports retaining the established ATP-hydrolysis annotation on independent evidence, without claiming full verification of the inaccessible original. The 5′–3′ DNA-helicase annotations describe the separately tested unwinding function; they do not replace this chemistry or transfer XPB translocation or CDK7 kinase activity to XPD. |
| GO:0016887 ATP hydrolysis activity | IEA GO_REF:0000116 | ACCEPT | Summary: Human XPD directly hydrolyzes ATP; this activity is experimentally separable from productive DNA unwinding. Reason: Official Rhea:13065 describes ATP + water → ADP + phosphate + proton, matching GO:0016887. Independently inspected human XPD experiments in PMID:19934020 Figure 5C and the ATPase Methods directly support ATP hydrolysis and distinguish it from productive DNA unwinding in the tested variants. Retain the correct reaction mapping alongside the directional DNA-helicase annotations. This is XPD ATPase chemistry, not kinase catalysis by the associated CDK7 subunit. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0030674 protein-macromolecule adaptor activity | IPI PMID:11445587 A yeast four-hybrid system identifies Cdk-activating kinase ... | MODIFY | Summary: XPD provides a protein-scaffold connection between CAK and the TFIIH core. Reason: The complete PMID:11445587 paper was not recovered, although original indexed Methods/initial Results and the verified abstract support the reported mapping. Independent native human CAK–ERCC2 isolation/reconstitution in PMID:8692842 Figures 3–6 and human XPD/p44 interaction/complex assays in PMID:19934020 Figure 4 substantiate the protein-complex scaffold role. Current GO:0030674 describes coordinated contact between macromolecules and does not imply XPD kinase activity. Current GO:0030674 explicitly distinguishes integral protein-complex scaffolds from generic adaptors. XPD is an integral TFIIH subunit whose human assembly/reconstitution and interface evidence supports holding the p44-associated core and CAK module together. Refine this structural role to GO:0140378 protein complex scaffold activity. This does not claim transient recruitment of a particular kinase substrate, kinase catalysis by XPD, or equivalent function of the 405-residue natural product. Proposed replacements: protein complex scaffold activity Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0035315 hair cell differentiation | IMP PMID:11335038 Trichothiodystrophy, a transcription syndrome. | UNDECIDED | Summary: The hair-cell differentiation annotation requires source and cell-type clarification. Reason: Only the verified abstract of PMID:11335038 was accessible. It describes trichothiodystrophy and hair dysplasia. Current GO:0035315 has both epidermal cell differentiation (GO:0009913) and neuron differentiation (GO:0030182) as direct is_a parents; its generic definition and specialized descendants do not make every use an inner-ear sensory-cell claim. Without the original discussion and primary evidence, the implicated cell type and a contribution to its differentiation remain unresolved. Preserve uncertainty rather than infer a terminology error from hair dysplasia alone. Supporting Evidence: |
| GO:0043139 5'-3' DNA helicase activity | EXP PMID:31253769 Structural basis of TFIIH activation for nucleotide excision... | ACCEPT | Summary: Human XPD catalyzes ATP-driven 5′–3′ DNA unwinding within TFIIH. Reason: PMID:31253769 Figure 1, Supplementary Figure 1b/c and the helicase Methods directly establish polarity and loss of unwinding with XPD K48R. Recombinant human full-length XPD is expressed with the other TFIIH core subunits in insect cells and purified after affinity-tag removal. Opposite-polarity controls and the separate XPB triplex-displacement assay prevent conflating the two ATPases. No equivalence is inferred for the 405-aa natural product. Supporting Evidence: |
| GO:0043139 5'-3' DNA helicase activity | EXP PMID:8413672 Human xeroderma pigmentosum group D gene encodes a DNA helic... | ACCEPT | Summary: XPD has experimentally established 5′–3′ DNA helicase activity. Reason: The complete originally cited article was not recovered. Independent human XPD-dependent unwinding in PMID:19934020 Figure 5B and directional/K48R controls in PMID:31253769 Supplementary Figure 1b establish this activity directly. Preserve the original source and evidence code; do not infer wrong-gene attribution from an XPB-focused title or transfer XPB translocase results to XPD. |
| GO:0043139 5'-3' DNA helicase activity | IDA PMID:11445587 A yeast four-hybrid system identifies Cdk-activating kinase ... | ACCEPT | Summary: XPD has experimentally established 5′–3′ DNA helicase activity. Reason: The complete originally cited article was not recovered. Independent human XPD-dependent unwinding in PMID:19934020 Figure 5B and directional/K48R controls in PMID:31253769 Supplementary Figure 1b establish this activity directly. Preserve the original source and evidence code; do not infer wrong-gene attribution from an XPB-focused title or transfer XPB translocase results to XPD. |
| GO:0043139 5'-3' DNA helicase activity | IDA PMID:8663148 A 3' --> 5' XPB helicase defect in repair/transcription fact... | ACCEPT | Summary: XPD has experimentally established 5′–3′ DNA helicase activity. Reason: The complete originally cited article was not recovered. Independent human XPD-dependent unwinding in PMID:19934020 Figure 5B and directional/K48R controls in PMID:31253769 Supplementary Figure 1b establish this activity directly. Preserve the original source and evidence code; do not infer wrong-gene attribution from an XPB-focused title or transfer XPB translocase results to XPD. |
| GO:0043139 5'-3' DNA helicase activity | IEA GO_REF:0000003 | ACCEPT | Summary: Human XPD has 5′–3′ DNA helicase activity. Reason: The official EC5.6.2.3 entry specifies ATP-coupled 5′–3′ DNA unwinding and lists human ERCC2; its generic replication-fork examples do not establish a replication-specific role for ERCC2. Direct human XPD/TFIIH unwinding assays in PMID:19934020 Figure 5 and PMID:31253769 Figure 1 independently establish the activity and distinguish XPD from XPB motor behavior. These full-length/recombinant-complex assays do not resolve the 405-aa natural product. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0043139 5'-3' DNA helicase activity | TAS Reactome:R-HSA-6782131 | ACCEPT | Summary: Human XPD has 5′–3′ DNA helicase activity. Reason: Official Reactome event6782131 has catalyst6782120 with activeUnit67443 (human P18074) and GO:0043139 activity. The event describes a proposed TC-NER backtracking mechanism; that detailed model is not required to accept the substrate/directionality annotation. Direct human XPD/TFIIH unwinding assays in PMID:19934020 Figure 5 and PMID:31253769 Figure 1 independently establish the activity and distinguish XPD from XPB motor behavior. These full-length/recombinant-complex assays do not resolve the 405-aa natural product. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0048568 embryonic organ development | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Developmental expression does not by itself establish embryonic organ-development participation. Reason: The rat donor A0A8I6AH26 carries IEP GO:0048568 from PMID:11425516. Its actual publisher-permitted original reprint in thesis Chapter 2 reports ERCC2/XPD RNA and protein in Sprague-Dawley embryo/yolk-sac samples at gestational days 10–12 (Methods pp62–68; Results pp69–76; Figures 2.2–2.6). The teratogen experiments measure embryo phenotypes and repair-gene expression, but do not perturb XPD specifically or assign it a developmental step. The source supports developmental expression and a plausible repair requirement; propagation to human organ-development participation exceeds the demonstrated source role. This does not claim ERCC2 cannot contribute to development or invalidate its established repair function. Propagation Review Root cause: SOURCE WEAK OR INFERRED Failure modes: ROLE CONFLATION Sources checked: UniProtKB:A0A8I6AH26 SOURCE WEAK OR INFERRED Actual rat IEP source measures developmental expression, not an XPD-specific contribution to organ development. ensembl:ENSRNOP00000092445 SUPPORTS TRANSFER Ortholog identity is verified; the limitation concerns the developmental assertion, not donor identity. Supporting Evidence: file:human/ERCC2/ERCC2-source-guide.md |
| GO:0070516 CAK-ERCC2 complex | IDA PMID:11445587 A yeast four-hybrid system identifies Cdk-activating kinase ... | ACCEPT | Summary: Human XPD can form a CAK–ERCC2 complex. Reason: Actual native human fractionation and coimmunoprecipitation in PMID:8692841 Figure 2B/C and PMID:8692842 Figure 3d establish CAK-associated XPD separately from the other TFIIH core components. These support the current GO complex definition; transcript-reconstitution experiments give the association functional relevance. Isolated-complex detection does not imply that every nuclear or cytoplasmic XPD molecule is in this state. |
| GO:0070516 CAK-ERCC2 complex | IDA PMID:8692841 Isolation and characterization of two human transcription fa... | ACCEPT | Summary: Human XPD can form a CAK–ERCC2 complex. Reason: Actual native human fractionation and coimmunoprecipitation in PMID:8692841 Figure 2B/C and PMID:8692842 Figure 3d establish CAK-associated XPD separately from the other TFIIH core components. These support the current GO complex definition; transcript-reconstitution experiments give the association functional relevance. Isolated-complex detection does not imply that every nuclear or cytoplasmic XPD molecule is in this state. |
| GO:0070516 CAK-ERCC2 complex | IDA PMID:8692842 Human cyclin-dependent kinase-activating kinase exists in th... | ACCEPT | Summary: Human XPD can form a CAK–ERCC2 complex. Reason: Actual native human fractionation and coimmunoprecipitation in PMID:8692841 Figure 2B/C and PMID:8692842 Figure 3d establish CAK-associated XPD separately from the other TFIIH core components. These support the current GO complex definition; transcript-reconstitution experiments give the association functional relevance. Isolated-complex detection does not imply that every nuclear or cytoplasmic XPD molecule is in this state. |
| GO:0071817 MMXD complex | IDA PMID:20797633 MMXD, a TFIIH-independent XPD-MMS19 protein complex involved... | UNDECIDED | Summary: The specific MMXD complex assignment remains incompletely resolved. Reason: PMID:20797633 explicitly reports an XPD–MMS19–MIP18–CIAO1–ANT2 complex, but its operative purification and stoichiometric evidence were not recovered. Independently inspected papers establish XPD client association with CIA proteins but do not establish equivalence to the complete named MMXD assembly. Retain uncertainty instead of denying the published complex from its abstract. |
| GO:1901990 regulation of mitotic cell cycle phase transition | IMP PMID:17088560 DNA nucleotide excision repair-dependent signaling to checkp... | KEEP AS NON CORE | Summary: XPD contributes to UV-induced checkpoint signaling in a cellular-context-dependent manner. Reason: PMID:17088560 Figure 4B/C and Figure 5 examine primary human XPD-mutant fibroblasts and show altered Chk1/p53 checkpoint phosphorylation, with distinct XP-D/CS behavior. This supports contextual regulation through NER lesion processing. The measured endpoint is checkpoint activation rather than phase-transition kinetics, and XPD is not the checkpoint kinase; retain the regulatory annotation as non-core with these limits. Supporting Evidence: |
Loading supporting content…
Download this section (compressed HTML)Q: Is the natural 405-residue P18074-2 product expressed as a stable protein, and can it assemble into TFIIH or perform any ATPase, helicase or scaffold function?
Q: Which original microscopy, purification and perturbation controls in PMID:20797633 establish XPD spindle localization and a complete MMXD assembly separately from transient CIA-client complexes?
Q: What exact ERCC2–GTF2H2C record in PMID:25416956 and ERCC2–BRAF WT-versus-mutant record in PMID:35512704 support the imported interaction assertions?
Q: Which cell type and primary differentiation experiment underlie the hair-cell annotation from PMID:11335038, given the current epidermal-cell and neuron-differentiation parents of GO:0035315?
Q: How does purified human XPD discriminate damaged DNA under controlled nucleotide and DNA-topology conditions, beyond the ancestral yeast RAD3 evidence and lesion-free human TFIIH structures?
Q: Which human transcription-coupled-repair experiments distinguish XPD DNA unwinding from XPB translocation and directly test the proposed RNA-polymerase-backtracking steps?
Loading supporting content…
Download this section (compressed HTML)Loading supporting content…
Download this section (compressed HTML)Loading supporting content…
Download this section (compressed HTML)Loading supporting content…
Download this section (compressed HTML)