ID   FXL13_HUMAN             Reviewed;         735 AA.
AC   Q8NEE6; C9J565; C9J566; Q6UVW7; Q6UVW8; Q75MN5; Q86UJ5; Q8N7Y4; Q8TCL2;
AC   Q8WUF9; Q8WUG0;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2010, sequence version 3.
DT   10-JUN-2026, entry version 177.
DE   RecName: Full=F-box and leucine-rich repeat protein 13 {ECO:0000303|PubMed:29348145};
DE   AltName: Full=Dynein regulatory complex subunit 6;
DE   AltName: Full=F-box/LRR-repeat protein 13;
GN   Name=FBXL13; Synonyms=DRC6 {ECO:0000250|UniProtKB:Q8CDU4}, FBL13;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4).
RX   PubMed=15820312; DOI=10.1016/j.ygeno.2005.01.013;
RA   Curtiss N.P., Bonifas J.M., Lauchle J.O., Balkman J.D., Kratz C.P.,
RA   Emerling B.M., Green E.D., Le Beau M.M., Shannon K.M.;
RT   "Isolation and analysis of candidate myeloid tumor suppressor genes from a
RT   commonly deleted segment of 7q22.";
RL   Genomics 85:600-607(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT MET-74.
RC   TISSUE=Testis;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12853948; DOI=10.1038/nature01782;
RA   Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA   Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA   Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA   Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA   Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA   Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA   Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA   Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA   Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA   Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA   Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA   Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA   Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA   Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA   Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA   Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA   Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA   Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA   McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA   Wilson R.K.;
RT   "The DNA sequence of human chromosome 7.";
RL   Nature 424:157-164(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND VARIANTS MET-74 AND
RP   GLY-692.
RC   TISSUE=Kidney, Liver, and Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 285-735.
RC   TISSUE=Testis;
RX   PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA   Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA   Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA   Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA   Wiemann S., Schupp I.;
RT   "The full-ORF clone resource of the German cDNA consortium.";
RL   BMC Genomics 8:399-399(2007).
RN   [6]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=29348145; DOI=10.15252/embr.201744799;
RA   Fung E., Richter C., Yang H.B., Schaeffer I., Fischer R., Kessler B.M.,
RA   Bassermann F., D'Angiolella V.;
RT   "FBXL13 directs the proteolysis of CEP192 to regulate centrosome
RT   homeostasis and cell migration.";
RL   EMBO Rep. 19:0-0(2018).
CC   -!- FUNCTION: Substrate-recognition component of the SCF (SKP1-CUL1-F-box
CC       protein)-type E3 ubiquitin ligase complex. Component of the nexin-
CC       dynein regulatory complex (N-DRC), a key regulator of ciliary/flagellar
CC       motility which maintains the alignment and integrity of the distal
CC       axoneme and regulates microtubule sliding in motile axonemes.
CC       Specifically targets CEP192 isoform 3 for ubiquitin-mediated
CC       proteolysis and thereby acts as a regulator of microtubule nucleation
CC       activity (PubMed:29348145). {ECO:0000250|UniProtKB:A8JHD7,
CC       ECO:0000269|PubMed:29348145}.
CC   -!- SUBUNIT: Component of the nexin-dynein regulatory complex (N-DRC).
CC       Directly interacts with SKP1 and CUL1. Interacts with DRC5.
CC       {ECO:0000250, ECO:0000250|UniProtKB:A8JHD7,
CC       ECO:0000250|UniProtKB:Q8CDU4}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, flagellum axoneme
CC       {ECO:0000250|UniProtKB:A8JHD7}. Cytoplasm, cytoskeleton, microtubule
CC       organizing center, centrosome {ECO:0000269|PubMed:29348145}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=4;
CC       Name=1;
CC         IsoId=Q8NEE6-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q8NEE6-2; Sequence=VSP_013004;
CC       Name=3;
CC         IsoId=Q8NEE6-3; Sequence=VSP_013005;
CC       Name=4;
CC         IsoId=Q8NEE6-4; Sequence=VSP_013003;
CC   -!- SIMILARITY: Belongs to the DRC6 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAD28506.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AY359238; AAR13262.1; -; mRNA.
DR   EMBL; AY359239; AAR13263.1; -; mRNA.
DR   EMBL; AK097537; BAC05092.1; -; mRNA.
DR   EMBL; AC005250; AAP22333.1; -; Genomic_DNA.
DR   EMBL; AC006477; AAS00354.1; -; Genomic_DNA.
DR   EMBL; AC073127; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC020572; AAH20572.2; -; mRNA.
DR   EMBL; BC020575; AAH20575.2; -; mRNA.
DR   EMBL; BC031285; AAH31285.1; -; mRNA.
DR   EMBL; AL713709; CAD28506.1; ALT_INIT; mRNA.
DR   CCDS; CCDS5726.1; -. [Q8NEE6-1]
DR   RefSeq; NP_001104508.1; NM_001111038.2. [Q8NEE6-3]
DR   RefSeq; NP_001274079.1; NM_001287150.2. [Q8NEE6-2]
DR   RefSeq; NP_659469.3; NM_145032.3. [Q8NEE6-1]
DR   RefSeq; XP_011514232.1; XM_011515930.3. [Q8NEE6-1]
DR   RefSeq; XP_016867342.1; XM_017011853.2. [Q8NEE6-1]
DR   AlphaFoldDB; Q8NEE6; -.
DR   SMR; Q8NEE6; -.
DR   BioGRID; 128792; 42.
DR   ComplexPortal; CPX-2241; SCF E3 ubiquitin ligase complex, FBXL13 variant.
DR   ComplexPortal; CPX-8086; Nexin-dynein regulatory complex.
DR   FunCoup; Q8NEE6; 130.
DR   IntAct; Q8NEE6; 5.
DR   MINT; Q8NEE6; -.
DR   NDEx; IQUERY-CP-FBXL13; 1 NDEx IQuery Curated Pathway.
DR   STRING; 9606.ENSP00000321927; -.
DR   GlyGen; Q8NEE6; 1 site, 1 O-linked glycan (1 site).
DR   iPTMnet; Q8NEE6; -.
DR   PhosphoSitePlus; Q8NEE6; -.
DR   SwissPalm; Q8NEE6; -.
DR   BioMuta; FBXL13; -.
DR   DMDM; 311033450; -.
DR   jPOST; Q8NEE6; -.
DR   MassIVE; Q8NEE6; -.
DR   PaxDb; 9606-ENSP00000321927; -.
DR   PeptideAtlas; Q8NEE6; -.
DR   ProteomicsDB; 73152; -. [Q8NEE6-1]
DR   ProteomicsDB; 73153; -. [Q8NEE6-2]
DR   ProteomicsDB; 73154; -. [Q8NEE6-3]
DR   ProteomicsDB; 73155; -. [Q8NEE6-4]
DR   Antibodypedia; 31140; 105 antibodies from 21 providers.
DR   DNASU; 222235; -.
DR   Ensembl; ENST00000313221.8; ENSP00000321927.4; ENSG00000161040.20. [Q8NEE6-1]
DR   GeneID; 222235; -.
DR   KEGG; hsa:222235; -.
DR   UCSC; uc003vaq.3; human. [Q8NEE6-1]
DR   AGR; HGNC:21658; -.
DR   ClinPGx; PA134938720; -.
DR   CTD; 222235; -.
DR   DisGeNET; 222235; -.
DR   GeneCards; FBXL13; -.
DR   HGNC; HGNC:21658; FBXL13.
DR   HPA; ENSG00000161040; Tissue enhanced (stomach, testis).
DR   MIM; 609080; gene.
DR   OpenTargets; ENSG00000161040; -.
DR   VEuPathDB; HostDB:ENSG00000161040; -.
DR   eggNOG; KOG1947; Eukaryota.
DR   GeneTree; ENSGT00940000160224; -.
DR   HOGENOM; CLU_012282_0_0_1; -.
DR   InParanoid; Q8NEE6; -.
DR   OMA; ARCHYLH; -.
DR   OrthoDB; 61560at2759; -.
DR   PAN-GO; Q8NEE6; 2 GO annotations based on evolutionary models.
DR   PhylomeDB; Q8NEE6; -.
DR   PathwayCommons; Q8NEE6; -.
DR   Reactome; R-HSA-8951664; Neddylation.
DR   Reactome; R-HSA-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR   SignaLink; Q8NEE6; -.
DR   Agora; ENSG00000161040; -.
DR   BioGRID-ORCS; 222235; 18 hits in 1195 CRISPR screens.
DR   ChiTaRS; FBXL13; human.
DR   GenomeRNAi; 222235; -.
DR   Pharos; Q8NEE6; Tbio.
DR   PRO; PR:Q8NEE6; -.
DR   Proteomes; UP000005640; Chromosome 7.
DR   RNAct; Q8NEE6; protein.
DR   Bgee; ENSG00000161040; Expressed in sperm and 110 other cell types or tissues.
DR   ExpressionAtlas; Q8NEE6; baseline and differential.
DR   GO; GO:0005930; C:axoneme; NAS:ComplexPortal.
DR   GO; GO:0005813; C:centrosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0005829; C:cytosol; TAS:Reactome.
DR   GO; GO:0019005; C:SCF ubiquitin ligase complex; IBA:GO_Central.
DR   GO; GO:0003341; P:cilium movement; NAS:ComplexPortal.
DR   GO; GO:0030317; P:flagellated sperm motility; NAS:ComplexPortal.
DR   GO; GO:0010824; P:regulation of centrosome duplication; NAS:ComplexPortal.
DR   GO; GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR   CDD; cd22124; F-box_FBXL13; 1.
DR   FunFam; 3.80.10.10:FF:000134; F-box and leucine rich repeat protein 13; 1.
DR   FunFam; 3.80.10.10:FF:000266; F-box and leucine rich repeat protein 13; 1.
DR   FunFam; 3.80.10.10:FF:000291; F-box and leucine rich repeat protein 13; 1.
DR   Gene3D; 3.80.10.10; Ribonuclease Inhibitor; 3.
DR   InterPro; IPR036047; F-box-like_dom_sf.
DR   InterPro; IPR001810; F-box_dom.
DR   InterPro; IPR057207; FBXL15_LRR.
DR   InterPro; IPR006553; Leu-rich_rpt_Cys-con_subtyp.
DR   InterPro; IPR032675; LRR_dom_sf.
DR   PANTHER; PTHR13318:SF240; F-BOX AND LEUCINE-RICH REPEAT PROTEIN 13; 1.
DR   PANTHER; PTHR13318; PARTNER OF PAIRED, ISOFORM B-RELATED; 1.
DR   Pfam; PF12937; F-box-like; 1.
DR   Pfam; PF25372; LRR_FBXL15; 2.
DR   SMART; SM00367; LRR_CC; 14.
DR   SUPFAM; SSF81383; F-box domain; 1.
DR   SUPFAM; SSF52047; RNI-like; 2.
DR   PROSITE; PS50181; FBOX; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Cell projection; Cilium; Cytoplasm; Cytoskeleton;
KW   Flagellum; Leucine-rich repeat; Proteomics identification;
KW   Reference proteome; Repeat; Ubl conjugation pathway.
FT   CHAIN           1..735
FT                   /note="F-box and leucine-rich repeat protein 13"
FT                   /id="PRO_0000119859"
FT   DOMAIN          152..198
FT                   /note="F-box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT   REPEAT          230..254
FT                   /note="LRR 1"
FT   REPEAT          255..280
FT                   /note="LRR 2"
FT   REPEAT          281..305
FT                   /note="LRR 3"
FT   REPEAT          306..333
FT                   /note="LRR 4"
FT   REPEAT          334..359
FT                   /note="LRR 5"
FT   REPEAT          360..385
FT                   /note="LRR 6"
FT   REPEAT          386..406
FT                   /note="LRR 7"
FT   REPEAT          410..435
FT                   /note="LRR 8"
FT   REPEAT          436..460
FT                   /note="LRR 9"
FT   REPEAT          461..488
FT                   /note="LRR 10"
FT   REPEAT          489..514
FT                   /note="LRR 11"
FT   REPEAT          515..538
FT                   /note="LRR 12"
FT   REPEAT          539..563
FT                   /note="LRR 13"
FT   REPEAT          564..589
FT                   /note="LRR 14"
FT   REPEAT          590..615
FT                   /note="LRR 15"
FT   REPEAT          616..641
FT                   /note="LRR 16"
FT   REPEAT          642..667
FT                   /note="LRR 17"
FT   REGION          682..703
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        682..692
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         337..618
FT                   /note="Missing (in isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:15820312"
FT                   /id="VSP_013003"
FT   VAR_SEQ         546..573
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_013004"
FT   VAR_SEQ         574..618
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:15820312"
FT                   /id="VSP_013005"
FT   VARIANT         74
FT                   /note="I -> M (in dbSNP:rs7805950)"
FT                   /evidence="ECO:0000269|PubMed:14702039,
FT                   ECO:0000269|PubMed:15489334"
FT                   /id="VAR_021480"
FT   VARIANT         313
FT                   /note="G -> A (in dbSNP:rs17135923)"
FT                   /id="VAR_031426"
FT   VARIANT         535
FT                   /note="I -> V (in dbSNP:rs17135873)"
FT                   /id="VAR_031427"
FT   VARIANT         565
FT                   /note="Y -> C (in dbSNP:rs17136118)"
FT                   /id="VAR_031428"
FT   VARIANT         692
FT                   /note="D -> G (in dbSNP:rs17852944)"
FT                   /evidence="ECO:0000269|PubMed:15489334"
FT                   /id="VAR_031429"
FT   CONFLICT        110
FT                   /note="I -> V (in Ref. 1; AAR13262)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        337
FT                   /note="I -> V (in Ref. 1; AAR13263)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        496
FT                   /note="S -> F (in Ref. 5; CAD28506)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        674
FT                   /note="K -> E (in Ref. 1; AAR13263)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   735 AA;  83924 MW;  27F92DC4693C2581 CRC64;
     MTPELMIKAC SFYTGHLVKT HFCTWRDIAR TNENVVLAEK MNRAVTCYNF RLQKSVFHHW
     HSYMEDQKEK LKNILLRIQQ IIYCHKLTII LTKWRNTARH KSKKKEDELI LKHELQLKKW
     KNRLILKRAA AEESNFPERS SSEVFLVDET LKCDISLLPE RAILQIFFYL SLKDVIICGQ
     VNHAWMLMTQ LNSLWNAIDF SSVKNVIPDK YIVSTLQRWR LNVLRLNFRG CLLRPKTFRS
     VSHCRNLQEL NVSDCPTFTD ESMRHISEGC PGVLCLNLSN TTITNRTMRL LPRHFHNLQN
     LSLAYCRRFT DKGLQYLNLG NGCHKLIYLD LSGCTQISVQ GFRYIANSCT GIMHLTINDM
     PTLTDNCVKA LVEKCSRITS LVFTGAPHIS DCTFRALSAC KLRKIRFEGN KRVTDASFKF
     IDKNYPNLSH IYMADCKGIT DSSLRSLSPL KQLTVLNLAN CVRIGDMGLK QFLDGPASMR
     IRELNLSNCV RLSDASVMKL SERCPNLNYL SLRNCEHLTA QGIGYIVNIF SLVSIDLSGT
     DISNEGLNVL SRHKKLKELS VSECYRITDD GIQAFCKSSL ILEHLDVSYC SQLSDMIIKA
     LAIYCINLTS LSIAGCPKIT DSAMEMLSAK CHYLHILDIS GCVLLTDQIL EDLQIGCKQL
     RILKMQYCTN ISKKAAQRMS SKVQQQEYNT NDPPRWFGYD REGNPVTELD NITSSKGALE
     LTVKKSTYSS EDQAA
//