ID   FXL14_HUMAN             Reviewed;         418 AA.
AC   Q8N1E6;
DT   07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   10-JUN-2026, entry version 175.
DE   RecName: Full=F-box/LRR-repeat protein 14;
DE   AltName: Full=F-box and leucine-rich repeat protein 14;
GN   Name=FBXL14; Synonyms=FBL14;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Lung;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [2]
RP   FUNCTION, INTERACTION WITH SNAI1, SUBCELLULAR LOCATION, AND INDUCTION BY
RP   HYPOXIA.
RX   PubMed=19955572; DOI=10.1074/jbc.m109.065995;
RA   Vinas-Castells R., Beltran M., Valls G., Gomez I., Garcia J.M.,
RA   Montserrat-Sentis B., Baulida J., Bonilla F., de Herreros A.G., Diaz V.M.;
RT   "The hypoxia-controlled FBXL14 ubiquitin ligase targets SNAIL1 for
RT   proteasome degradation.";
RL   J. Biol. Chem. 285:3794-3805(2010).
CC   -!- FUNCTION: Substrate-recognition component of some SCF (SKP1-CUL1-F-box
CC       protein)-type E3 ubiquitin-protein ligase complexes. The SCF(FBXL14)
CC       complex acts by mediating ubiquitination and subsequent degradation of
CC       SNAI1. {ECO:0000269|PubMed:19955572}.
CC   -!- SUBUNIT: Part of a SCF (SKP1-cullin-F-box) ubiquitin-protein ligase
CC       complex. Interacts with SKP1 and CUL1 (By similarity). Interacts with
CC       SNAI1; the interaction requires the phosphorylation of the two serine
CC       residues in the substrate destruction motif D-S-G-X(2,3,4)-S.
CC       {ECO:0000250, ECO:0000269|PubMed:19955572}.
CC   -!- INTERACTION:
CC       Q8N1E6; P63208: SKP1; NbExp=7; IntAct=EBI-6425532, EBI-307486;
CC       Q8N1E6; O95863: SNAI1; NbExp=2; IntAct=EBI-6425532, EBI-1045459;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:19955572}.
CC   -!- INDUCTION: Down-regulated by hypoxia. {ECO:0000269|PubMed:19955572}.
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DR   EMBL; BC028132; AAH28132.1; -; mRNA.
DR   CCDS; CCDS8509.1; -.
DR   RefSeq; NP_689654.1; NM_152441.3.
DR   AlphaFoldDB; Q8N1E6; -.
DR   SMR; Q8N1E6; -.
DR   BioGRID; 126870; 40.
DR   ComplexPortal; CPX-2319; SCF E3 ubiquitin ligase complex, FBXL14 variant.
DR   FunCoup; Q8N1E6; 655.
DR   IntAct; Q8N1E6; 43.
DR   MINT; Q8N1E6; -.
DR   STRING; 9606.ENSP00000344855; -.
DR   GlyGen; Q8N1E6; 1 site, 1 O-linked glycan (1 site).
DR   iPTMnet; Q8N1E6; -.
DR   PhosphoSitePlus; Q8N1E6; -.
DR   BioMuta; FBXL14; -.
DR   DMDM; 48428083; -.
DR   jPOST; Q8N1E6; -.
DR   MassIVE; Q8N1E6; -.
DR   PaxDb; 9606-ENSP00000344855; -.
DR   PeptideAtlas; Q8N1E6; -.
DR   ProteomicsDB; 71590; -.
DR   Pumba; Q8N1E6; -.
DR   Antibodypedia; 41744; 107 antibodies from 23 providers.
DR   DNASU; 144699; -.
DR   Ensembl; ENST00000339235.4; ENSP00000344855.3; ENSG00000171823.8.
DR   GeneID; 144699; -.
DR   KEGG; hsa:144699; -.
DR   MANE-Select; ENST00000339235.4; ENSP00000344855.3; NM_152441.3; NP_689654.1.
DR   UCSC; uc001qjh.3; human.
DR   AGR; HGNC:28624; -.
DR   ClinPGx; PA134979350; -.
DR   CTD; 144699; -.
DR   DisGeNET; 144699; -.
DR   GeneCards; FBXL14; -.
DR   HGNC; HGNC:28624; FBXL14.
DR   HPA; ENSG00000171823; Low tissue specificity.
DR   MIM; 609081; gene.
DR   OpenTargets; ENSG00000171823; -.
DR   VEuPathDB; HostDB:ENSG00000171823; -.
DR   eggNOG; KOG1947; Eukaryota.
DR   GeneTree; ENSGT00940000159857; -.
DR   HOGENOM; CLU_016072_7_0_1; -.
DR   InParanoid; Q8N1E6; -.
DR   OMA; DQALVHI; -.
DR   OrthoDB; 2585512at2759; -.
DR   PAN-GO; Q8N1E6; 2 GO annotations based on evolutionary models.
DR   PhylomeDB; Q8N1E6; -.
DR   PathwayCommons; Q8N1E6; -.
DR   Reactome; R-HSA-8951664; Neddylation.
DR   Reactome; R-HSA-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR   SignaLink; Q8N1E6; -.
DR   SIGNOR; Q8N1E6; -.
DR   Agora; ENSG00000171823; -.
DR   BioGRID-ORCS; 144699; 15 hits in 1194 CRISPR screens.
DR   ChiTaRS; FBXL14; human.
DR   GenomeRNAi; 144699; -.
DR   Pharos; Q8N1E6; Tbio.
DR   PRO; PR:Q8N1E6; -.
DR   Proteomes; UP000005640; Chromosome 12.
DR   RNAct; Q8N1E6; protein.
DR   Bgee; ENSG00000171823; Expressed in left testis and 114 other cell types or tissues.
DR   GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR   GO; GO:0005829; C:cytosol; TAS:Reactome.
DR   GO; GO:0019005; C:SCF ubiquitin ligase complex; IBA:GO_Central.
DR   GO; GO:0004842; F:ubiquitin-protein transferase activity; IDA:UniProtKB.
DR   GO; GO:0006355; P:regulation of DNA-templated transcription; NAS:ComplexPortal.
DR   GO; GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR   GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IDA:UniProtKB.
DR   CDD; cd22125; F-box_FBXL14; 1.
DR   FunFam; 3.80.10.10:FF:000051; F-box and leucine-rich repeat protein 14; 1.
DR   FunFam; 3.80.10.10:FF:000075; F-box/LRR-repeat protein 14 isoform X1; 1.
DR   FunFam; 1.20.1280.50:FF:000059; Partner of Paired; 1.
DR   Gene3D; 3.80.10.10; Ribonuclease Inhibitor; 2.
DR   InterPro; IPR036047; F-box-like_dom_sf.
DR   InterPro; IPR001810; F-box_dom.
DR   InterPro; IPR047932; FBXL14_F-box.
DR   InterPro; IPR057207; FBXL15_LRR.
DR   InterPro; IPR006553; Leu-rich_rpt_Cys-con_subtyp.
DR   InterPro; IPR032675; LRR_dom_sf.
DR   PANTHER; PTHR13318:SF190; PARTNER OF PAIRED, ISOFORM B; 1.
DR   PANTHER; PTHR13318; PARTNER OF PAIRED, ISOFORM B-RELATED; 1.
DR   Pfam; PF12937; F-box-like; 1.
DR   Pfam; PF25372; LRR_FBXL15; 2.
DR   SMART; SM00367; LRR_CC; 11.
DR   SUPFAM; SSF81383; F-box domain; 1.
DR   SUPFAM; SSF52047; RNI-like; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Leucine-rich repeat; Proteomics identification;
KW   Reference proteome; Repeat; Ubl conjugation pathway.
FT   CHAIN           1..418
FT                   /note="F-box/LRR-repeat protein 14"
FT                   /id="PRO_0000119861"
FT   DOMAIN          2..48
FT                   /note="F-box"
FT   REPEAT          144..163
FT                   /note="LRR 1"
FT   REPEAT          170..191
FT                   /note="LRR 2"
FT   REPEAT          203..225
FT                   /note="LRR 3"
FT   REPEAT          229..250
FT                   /note="LRR 4"
FT   REPEAT          254..275
FT                   /note="LRR 5"
FT   REGION          2..48
FT                   /note="Required for down-regulation of SNAI1"
FT   VARIANT         220
FT                   /note="L -> V (in dbSNP:rs35571553)"
FT                   /id="VAR_049033"
SQ   SEQUENCE   418 AA;  45886 MW;  5779961C8177779F CRC64;
     METHISCLFP ELLAMIFGYL DVRDKGRAAQ VCTAWRDAAY HKSVWRGVEA KLHLRRANPS
     LFPSLQARGI RRVQILSLRR SLSYVIQGMA NIESLNLSGC YNLTDNGLGH AFVQEIGSLR
     ALNLSLCKQI TDSSLGRIAQ YLKGLEVLEL GGCSNITNTG LLLIAWGLQR LKSLNLRSCR
     HLSDVGIGHL AGMTRSAAEG CLGLEQLTLQ DCQKLTDLSL KHISRGLTGL RLLNLSFCGG
     ISDAGLLHLS HMGSLRSLNL RSCDNISDTG IMHLAMGSLR LSGLDVSFCD KVGDQSLAYI
     AQGLDGLKSL SLCSCHISDD GINRMVRQMH GLRTLNIGQC VRITDKGLEL IAEHLSQLTG
     IDLYGCTRIT KRGLERITQL PCLKVLNLGL WQMTDSEKEA RGDFSPLFTV RTRGSSRR
//